International Journal of Reproductive Biomedicine最新文献

{"title":"Stereological and biochemical effects of thymoquinone on ovarian tissue toxicity induced by silver nanoparticles in NMRI mice: An experimental study.","authors":"Seyed Mohammad Ali Shariatzadeh, Fatemeh Salmani, Hossein Moghanlo, Monireh Mahmoodi","doi":"10.18502/ijrm.v22i7.16964","DOIUrl":"10.18502/ijrm.v22i7.16964","url":null,"abstract":"<p><strong>Background: </strong>The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of <i>Nigella sativa</i>.</p><p><strong>Objective: </strong>We evaluated the efficacy of TQ on ovarian tissue following toxicity induced by AgNPs in female mice.</p><p><strong>Materials and methods: </strong>24 female NMRI mice (5-6 wk, an average weight of 33 gr) were randomly divided into 4 groups (n = 6/each): control, AgNPs (500 mg/kg, gavage), TQ (2.5 mg/kg, intraperitoneal injection), and TQ+AgNPs. Mice were treated every day for 35 days. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), luteinizing hormone, and follicle-stimulating hormone were measured. The optical disector and stereological techniques were utilized to estimate the follicular count, their volume at different developmental stages, and the structure of ovarian tissue.</p><p><strong>Results: </strong>In the AgNPs group, the serum concentrations of TAC (p = 0.01), luteinizing hormone (p <math><mo><</mo></math> 0.001), follicle-stimulating hormone, the volume of corpus luteum (p <math><mo><</mo></math> 0.001), and the number of follicles decreased significantly compared to the control group. Nevertheless, AgNPs significantly increased the MDA level. In the TQ+AgNPs group compared to the AgNPs group, a significant decrease in MDA level (p <math><mo><</mo></math> 0.001) and a significant improvement in TAC (p = 0.03), and hormonal levels, the number of primary, preantral, and antral follicles (p = 0.04), and the volume of corpus luteum (p = 0.01) were observed.</p><p><strong>Conclusion: </strong>TQ improved the number of follicles by reducing oxidative stress and lipid peroxidation in AgNPs-damaged ovarian tissue.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"553-566"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive correlation between <i>Bax</i> and <i>Bcl-2</i> gene polymorphisms with the risk of endometriosis: A case-control study.","authors":"Arefe Edalatian Kharrazi, Forough Forghani, Danial Jahantigh, Saeedeh Ghazaey Zidanloo, Mahnaz Rezaei, Mohsen Taheri","doi":"10.18502/ijrm.v22i6.16796","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16796","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is a chronic, gynecological disorder, and the disease's pathogenesis is still debatable. Genes related to apoptosis have been revealed to be deregulated in endometriosis.</p><p><strong>Objective: </strong>This study investigates the relationship between polymorphic variants of <i>Bax -248G</i> <math><mo>></mo></math> <i>A</i> and <i>Bcl-2</i> -938C <math><mo>></mo></math> A promoter regions with endometriosis risk in an Iranian population.</p><p><strong>Materials and methods: </strong>In this case-control study, the polymorphisms of <i>Bax</i> -248G <math><mo>></mo></math> A and <i>Bcl</i>-2 -938C <math><mo>></mo></math> A promoter regions were analyzed in 127 Iranian cases and 125 controls who were referred to Ali-ibn-Abi Taleb Educational hospital, Zahedan, Iran between May 2022 and February 2023. The genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method.</p><p><strong>Results: </strong>The frequencies of mutant allele A carriers and the A allele of <i>Bax</i> -248G <math><mo>></mo></math> A polymorphism showed about 2-fold significant increase of endometriosis risk (p = 0.04; p = 0.01, respectively). The frequencies of the mutant genotype AA and A allele carriers of <i>Bcl-2</i> -938C <math><mo>></mo></math> A polymorphism were approximately 4 and 2.5-fold higher in endometriosis compared to the control women, which were highly significant (p <math><mo>></mo></math> 0.001). Moreover, the allele A frequency of <i>Bcl-2</i> -938C <math><mo>></mo></math> A was associated with a 2-fold higher risk of endometriosis (p <math><mo>></mo></math> 0.001). Furthermore, the combination effects of these 2 single nucleotide polymorphisms showed that women with <i>Bax -248G</i> <math><mo>></mo></math> <i>A</i> GGand <i>Bcl-2 -938C</i> <math><mo>></mo></math> <i>A</i> AA variant alleles were associated with about 5 times higher risk of endometriosis (p <math><mo>></mo></math> 0.001). Notably, a significant difference was observed in mutant allele distribution between minimal/mild (stage I and II) and moderate/severe (stage III and IV) women with endometriosis disease.</p><p><strong>Conclusion: </strong>The results of our study provide evidence that <i>Bcl-2</i> -938C <math><mo>></mo></math> A and <i>Bax</i> -248G <math><mo>></mo></math> A single nucleotide polymorphisms might be associated with the risk of endometriosis.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"451-462"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of genetic variations and protein expression of β-Microsemino protein in intrauterine insemination outcome of unexplained infertile men: A case-control study.","authors":"Elham Bagherian, Sahar Jokari, Parnaz Borjian Boroujeni, Kaveh Haratian, Marjan Sabbaghian, Anahita Mohseni Meybodi","doi":"10.18502/ijrm.v22i6.16799","DOIUrl":"10.18502/ijrm.v22i6.16799","url":null,"abstract":"<p><strong>Background: </strong>Intrauterine insemination (IUI) is often the first-line treatment for unexplained infertility. <math><mi>β</mi></math> -Microsemino protein (MSMB) is an abundant protein in seminal plasma that has an inhibitory effect on spontaneous acrosome reaction.</p><p><strong>Objective: </strong>The present study aimed to evaluate <i>MSMB</i> gene variations and protein expression on IUI success rate in unexplained infertile men.</p><p><strong>Materials and methods: </strong>A case-control study was performed on 100 unexplained infertile Iranian men referred to the Royan Institute, Tehran, Iran for IUI (50 men with IUI positive result [IUI+], and 50 men with IUI negative result [IUI-]). Couples with female infertility factors (such as hormonal disorders, infrequent menstrual period, abnormality in uterus, fallopian tubes, or ovaries) and men with infections of the male accessory glands, hypogonadotropic hypogonadism, clinical varicocele, retractile testis, genital trauma, drug use, or concurrent hormonal treatment Y chromosome microdeletions, and abnormal karyotype were excluded from the study. The polymerase chain reaction sequencing was performed for the promoter and the coding regions of <i>MSMB</i> functional domains. To study the protein expression, the total protein of sperm was extracted, and sandwich enzyme-linked immunosorbent assay was performed.</p><p><strong>Results: </strong>4 variations were detected (rs12770171, rs10993994, rs2075894, and rs4517463). None of them showed significant differences between the IUI+ and IUI- groups. The mean value of protein expression did not show any differences between the groups.</p><p><strong>Conclusion: </strong>In conclusion, there is no association between genetic variations of promoter and coding regions of <i>MSMB</i> functional domains as well as its expression with IUI success in unexplained infertile men.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"481-494"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of sperm <i>BAX</i> and <i>BCL-2</i> gene expression with reproductive outcome in Oligoasthenoteratozoospermia cases undergoing intracytoplasmic sperm injection: A case-control study.","authors":"Fatemeh Babaei, Maryam Khoshsokhan Muzaffar, Rahil Jannatifar","doi":"10.18502/ijrm.v22i6.16797","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16797","url":null,"abstract":"<p><strong>Background: </strong>The B-cell lymphoma 2 (<i>BCL-2</i>) protein is one of the members of the <i>BCL-2</i> associated X (<i>BAX</i>) protein family that acts as an inducer of apoptosis.</p><p><strong>Objective: </strong>The present study aims to investigate the association between <i>BAX</i> and <i>BCL-2</i> gene expression with reproductive outcome, in cases undergoing intracytoplasmic sperm injection.</p><p><strong>Materials and methods: </strong>In this case-control study, 50 men were divided into healthy fertile and oligoasthenoteratozoospermic infertile men (n = 25/each). They were subjected to history taking, clinical examination, and semen analysis. Expression of <i>BAX</i> and <i>BCL-2</i> genes were measured using real-time polymerase chain reaction. The DNA fragmentation index was measured using the sperm chromatin dispersion assay technique. Using World Health Organization criteria, sperm parameters were evaluated.</p><p><strong>Results: </strong>Evaluation of apoptosis-related genes showed that oligoasthenoteratozoospermic significantly increased mRNA expression of <i>BAX</i>, and significantly decreased mRNA expression of <i>BCL-2</i>, when compared with control. Moreover, the <i>BAX/BCL-2</i> ratio was significantly higher in oligoasthenoteratozoospermic compared to the normozoospermic group (p = 0.01). Also, this study showed that the <i>BAX</i> and <i>BCL-2</i> genes expression had a significant correlation with sperm quality, and DNA fragmentation in the oligoasthenoteratozoospermic group (p = 0.01). The oligoasthenoteratozoospermic men, had a considerably lower proportion of fertilization rate and good-quality embryos at the cleavage stage than the normozoospermic subjects (p = 0.01). A significant correlation was observed between the expression of <i>BAX</i> and <i>BCL-2</i> genes, fertilization, and embryo quality (p = 0.01).</p><p><strong>Conclusion: </strong>We concluded that the sperm <i>BAX/BCL-2</i> ratio demonstrates a significant correlation with fertilization rate and embryo quality.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"463-472"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of sequential cleavage and blastocyst embryo transfer compared to single cleavage stage embryo transfer on assisted reproductive technology outcome: An RCT.","authors":"Nahid Homayoon, Sahereh Arabian, Esmat Mangoli, Fatemeh Bayati, Maryam Eftekhar","doi":"10.18502/ijrm.v22i6.16793","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16793","url":null,"abstract":"<p><strong>Background: </strong>Assisted reproductive technology (ART), offers hope for many infertile couples by increasing the chance of successful pregnancy. The success of in vitro fertilization depends on various factors, in which embryo transfer (ET) is one of the critical steps influencing in vitro fertilization success rates. Extended embryo culture and blastocyst-stage ET have been considered in ART due to their potential benefits including improved implantation rates.</p><p><strong>Objective: </strong>This study aimed to compare the outcomes of sequential ET vs single cleavage-stage ET in women undergoing a fresh ET cycle with a limited number of embryos.</p><p><strong>Materials and methods: </strong>This randomized clinical trial was conducted on 140 women undergoing infertility treatments and candidates for fresh ET at the Research and Clinical Center for Infertility, Yazd, Iran from August 2023 to January 2024. Women with a number of embryos from 2-5 ( <math><mo>≥</mo></math> 2 and <math><mo>≤</mo></math> 5 available embryos) were randomly divided into 2 groups: One group underwent sequential ET (one cleavage-stage ET followed by one blastocyst ET) and the other group underwent single-step 2 cleavage-stage ET. The primary outcome was clinical pregnancy, and the secondary outcome included chemical pregnancy and early abortion rates.</p><p><strong>Results: </strong>Our findings showed significantly higher rates of clinical (33.5% vs 13.6%, p = 0.003) and chemical (41.3% vs 18.2%, p = 0.004) pregnancies in the sequential ET group compared to the single-step cleavage ET group. The early abortion rate was higher in single-step cleavage ET (13% vs 44.4%, p = 0.053).</p><p><strong>Conclusion: </strong>Sequential fresh ET is a useful choice in women who have a limited number of embryos and can improve ART outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"433-440"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-free in vitro activation and autologous transplantation in infertile women with diminished ovarian reserve: An experimental pilot study.","authors":"Saghar Salehpour, Mahsa Kazemi, Sedigheh Hosseini, Nazanin Hajizadeh, Bahareh Karimi, Hajar Abbasi, Mitra Nemati, Samaneh Esmaeili, Hasti Ziaee","doi":"10.18502/ijrm.v22i6.16792","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16792","url":null,"abstract":"<p><strong>Background: </strong>Poor ovarian response and diminished ovarian reserves (DOR) significantly contribute to female infertility. Previous attempts have been made to enhance follicular growth and improve pregnancy outcomes in these participants.</p><p><strong>Objective: </strong>This study aimed to assess the efficacy of the in vitro drug-free activation technique of the ovarian reservation and in vitro fertilization stimulation cycle outcomes in DOR participants.</p><p><strong>Materials and methods: </strong>This pilot phase study investigated the impact of in vitro activation (IVA) on ovarian reservation and in vitro fertilization outcome in 10 infertile women with DOR from May to December 2023 at Taleghani Infertility Center, Tehran, Iran. Participants underwent general surgery and laparoscopy, involving the removal of a portion of one ovary, immediate transfer to the laboratory, dissection into small cubes, and subsequent re-implantation into the cases's ovary. The primary outcomes, include the count of retrieved oocytes, the number of oocytes reaching metaphase, and the secondary outcomes were the quantity and the number of embryos transferred, implantation rate, and occurrence of clinical pregnancy.</p><p><strong>Results: </strong>The study revealed a significant increase in the antral follicle count before and after IVA (p = 0.033). Before IVA, the median estradiol level was 93.5 (57.0), which reduced to 79.0 (35.0) after IVA, indicating a statistically significant difference. On average, 2.3 (0.8) oocytes were retrieved, among which 1.5 (0.7) were metaphase II oocytes. The observed pregnancy rate among the 2 cases was 22.2%.</p><p><strong>Conclusion: </strong>The current study suggests that IVA may positively impact follicular growth and pregnancy outcomes among women with DOR.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"425-432"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulatory roles of Smad2/3 protein and <i>SMURF2</i> gene expression in granulosa cells of germinal vesicle and metaphase II oocytes in polycystic ovarian syndrome: A case-control study.","authors":"Marzieh Ghorbani, Marzieh Sanoee Farimani, Iraj Khodadadi, Sina Mohagheghi, Iraj Amiri, Heidar Tayebinia","doi":"10.18502/ijrm.v22i6.16794","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16794","url":null,"abstract":"<p><strong>Background: </strong>The impaired functions of granulosa cells (GCs) in the delayed development and immaturity of oocytes have been reported in polycystic ovary syndrome (PCOs). Even with ovarian stimulation, a large number of oocytes in these patients are still in the stage germinal vesicle (GV).</p><p><strong>Objective: </strong>The levels of Smad2/3, phosphorylated Smad2/3 (P-Smad2/3), the expression of <i>SARA</i>, <i>Smad4</i>, and <i>SMURF2</i> genes in the GCs surrounding metaphase II (MII) or GV oocytes in PCOs women were investigated.</p><p><strong>Materials and methods: </strong>GCs of MII and GV oocytes were isolated from 38 women with PCOs and the expression levels of <i>SARA, Smad4,</i> and <i>SMURF2</i> in surrounding GCs of MII and GV oocytes were determined using reverse-transcription polymerase chain reaction<i>.</i> Also, Smad2/3 and P-Smad2/3 proteins were determined using western blotting.</p><p><strong>Results: </strong>The expression level of <i>SMURF2</i> was significantly higher in GCs surrounding GV oocytes compared with that of GCs encompassing MII oocytes (p <math><mo><</mo></math> 0.001). At the same time, no significant differences were observed in <i>SARA</i> and <i>Smad4</i> expression levels in GCs surrounding GV and MII oocytes. A lower level of P-Smad2/3 was also found in GCs GV oocytes compared with GCs of MII oocytes (p <math><mo><</mo></math> 0.001).</p><p><strong>Conclusion: </strong>It seems that P-Smad2/3 plays a role in oocyte development, and the downregulation of this protein is associated with a defect in the maturation of GV oocytes. On the other hand, the upregulation of the <i>SMURF2</i> gene also affects the growth process of GCs and the maturation of GV oocytes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"441-450"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometriosis and adverse pregnancy outcomes: A case-control study.","authors":"Fatemeh Shahmoradi, Ladan Haghighi, Marziyeh Noori, Roya Derakhshan, Neda Hashemi, Samaneh Rokhgireh","doi":"10.18502/ijrm.v22i6.16798","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16798","url":null,"abstract":"<p><strong>Background: </strong>The association between endometriosis and the outcome of pregnancy is one of the interesting topics. Endometriosis-related pain is alleviated with pregnancy; however, it is known to cause adverse outcomes in pregnancy. The main cause is systemic chronic inflammation caused by higher levels of cytokines, growth factors, and angiogenesis factors.</p><p><strong>Objective: </strong>This study aimed to clarify the relationship between endometriosis, deep endometriosis, adenomyosis, surgical treatment, and poor maternal consequences.</p><p><strong>Materials and methods: </strong>In this case-control study, data from 250 women who gave birth in Hazrat Rasoul Akram hospital, Tehran, Iran from February 2015 to December 2019 was extracted from the hospital information system in January 2020. Participants were divided into 2 groups: 125 women with endometriosis and 125 women without endometriosis. We looked at how endometriosis affected mothers and newborn babies. Data on pregnancy, delivery, and newborns of both groups was extracted.</p><p><strong>Results: </strong>The mean age of participants was 32.74 <math><mo>±</mo></math> 4.10 and 31.7 <math><mo>±</mo></math> 5.53 yr in endometriosis and control group, respectively. In terms of pregnancy complications, placenta previa, placenta accreta, placenta abruption, pre-eclampsia, gestational diabetes mellitus, and postpartum hemorrhage remarkably increased in the endometriosis group compared to the control group. Small for gestational age was significantly higher in rectal endometriosis than women without rectal endometriosis (p = 0.03). The neonatal intensive care unit admission rate was notably higher in infants of the endometriosis group compared to controls (40.7% vs. 24.8%, p = 0.009).</p><p><strong>Conclusion: </strong>Our findings showed women with endometriosis are at a higher risk for important adverse maternal outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"473-480"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the gene expression profiles of endometrial and trophoblastic cells in women with recurrent miscarriage: A bioinformatics approach.","authors":"Kambiz Ahmadi, Somayeh Reiisi, Zahra Habibi","doi":"10.18502/ijrm.v22i6.16800","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16800","url":null,"abstract":"<p><strong>Background: </strong>Recurrent miscarriage (RM) remains unsolved in <math><mo>></mo></math> 50% of patients and causes physical and psychological problems in women without specific risk factors for miscarriage. For a successful pregnancy, acceptance of the endometrium and invasion of trophoblast cells into the endometrium is necessary.</p><p><strong>Objective: </strong>This study aimed to use computational analysis to identify key genes and related pathways in endometrial and trophoblast cells derived from RM samples.</p><p><strong>Materials and methods: </strong>In this bioinformatics study, we explored the differential expression of genes in endometrial and trophoblast cells by analyzing the GSE165004 and GSE76862 datasets, respectively with the limma package in R software. Subsequently, overlapped genes between 2 datasets were selected, gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed. The overlapped genes were integrated to construct a protein-protein interaction network and hub genes selection.</p><p><strong>Results: </strong>We observed 41 overlapped genes between endometrial and trophoblast cells, and future analysis was accomplished in overlapped and nonoverlapped genes. Kyoto Encyclopedia of Genes and Genomes analysis indicated that overlapped genes were significantly enriched in the complement and coagulation cascades, pluripotency of stem cells, and synthesis and degradation of ketone bodies. Gene ontology analysis suggested that the genes were enriched in the cell cycle, apoptosis, and cell division. The top 10 genes included: <i>IRS1, FGF2, MAPK6, MAPK1, MAPK3, MAPK8, MAPK9, PLK1, PRKACA</i>, and <i>PRKCA</i> were identified from the PPI network.</p><p><strong>Conclusion: </strong>This study identified the key genes and potential molecular pathways underlying the development of RM. This could provide novel insights to determine the possible mechanisms and interventional strategies associated with miscarriage.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"495-506"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental accelerating testicular tissue recovery post-methotrexate treatment in rats: A promising role of Sertoli cell-conditioned medium: An experimental study.","authors":"Aref Delkhosh, Fatemeh Shabani, Masoud Delashoub","doi":"10.18502/ijrm.v22i4.16390","DOIUrl":"10.18502/ijrm.v22i4.16390","url":null,"abstract":"<p><strong>Background: </strong>Methotrexate (MET) is one of the most important chemotherapy agents used against various tumors and cancer diseases. One of the critical side effects of MET is inducing male infertility.</p><p><strong>Objective: </strong>The current study aimed to investigate Sertoli cell culture-conditioned medium (SCM) recovery effects on MET-induced conditions in rats.</p><p><strong>Materials and methods: </strong>30 mature male Wistar rats were randomly divided into 3 groups (n = 10). In the first group, rats received normal saline intraperitoneally. In the second group, animals received MET (10 mg/kg; intraperitoneally) once a week for 2 wk. The rats in the third group (MET+SCM) received MET and a single injection of SCM for 56 days post-MET administration. 56 days later, serum, epididymis, and testicular tissue samples were collected, and the animals were euthanized. Sperm parameters, serum levels of luteinizing hormone, follicle-stimulating hormone, and testosterone were examined. The testicular tissues were stained using hematoxylin and eosin solution, and histopathological changes were analyzed.</p><p><strong>Results: </strong>The MET-induced condition resulted in significant pathological changes in the testis, decreased hormone levels, and downregulated sperm parameters. However, SCM injection improved hormonal levels, testicular changes, and sperm parameters.</p><p><strong>Conclusion: </strong>It can be concluded that a single intra-testicular SCM injection accelerates male reproductive system recovery post-MET treatment.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 4","pages":"295-304"},"PeriodicalIF":1.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11255466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}