International Journal of Reproductive Biomedicine最新文献

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Fixed versus flexible gonadotropin releasing hormone antagonist protocol in women with polycystic ovary syndrome undergoing in vitro fertilization: An RCT. 多囊卵巢综合征妇女体外受精中促性腺激素释放激素拮抗剂固定方案与灵活方案的比较:一项 RCT 研究。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-10-14 eCollection Date: 2024-08-01 DOI: 10.18502/ijrm.v22i8.17230
Hanieh Fatehi, Robab Davar, Elham Nikfarjam, Fatemeh Bayati
{"title":"Fixed versus flexible gonadotropin releasing hormone antagonist protocol in women with polycystic ovary syndrome undergoing in vitro fertilization: An RCT.","authors":"Hanieh Fatehi, Robab Davar, Elham Nikfarjam, Fatemeh Bayati","doi":"10.18502/ijrm.v22i8.17230","DOIUrl":"10.18502/ijrm.v22i8.17230","url":null,"abstract":"<p><strong>Background: </strong>Despite the extensive use of the gonadotropin-releasing hormone (GnRH) antagonist protocol in treating infertile women, particularly those with polycystic ovary syndrome (PCOS), there have not been sufficient evidence to compare the flexible and fixed variants in in vitro fertilization (IVF) cycles.</p><p><strong>Objective: </strong>This study aims to assess the treatment outcomes of flexible and fixed types of GnRH-antagonist protocol for IVF in women with PCOS.</p><p><strong>Materials and methods: </strong>In this randomized clinical trial, 150 infertile women with PCOS, who were candidates for IVF, and referred to the Yazd Research and Clinical Center for Infertility, Yazd, Iran between October 2023 and February 2024 were included. Participants were divided into 2 groups (n = 75/each) based on the type of antagonist protocol (fixed or flexible). GnRH antagonist administration started on the 5 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> day of gonadotropin treatment in the fixed group. In the flexible group when there was at least one follicle 12-14 mm, GnRH antagonist was started. Finally, the number of metaphase II oocyte, the quality of embryos, the duration of the stimulation cycle, the dose of gonadotropin, the number of GnRH-antagonist, and the rate of ovarian hyperstimulation syndrome were evaluated.</p><p><strong>Results: </strong>No statistically significant difference was observed in terms of cycle length and the total dose of gonadotropin between groups. Nevertheless, a notable distinction was observed in the total number of oocytes (17.84 vs. 15.5, p = 0.023) and mature oocytes (13.64 vs. 11.83, p = 0.019) in the flexible group compared to the fixed group.</p><p><strong>Conclusion: </strong>In conclusion, the IVF outcomes are more favorable in women with PCOS undergoing the flexible GnRH-antagonist protocol compared to the fixed protocol.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 8","pages":"617-626"},"PeriodicalIF":1.6,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aspirin before the 11 th week of pregnancy to prevent pre-eclampsia. 在怀孕 11 周前服用阿司匹林,以预防先兆子痫。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-10-14 eCollection Date: 2024-08-01 DOI: 10.18502/ijrm.v22i8.17245
Marie-Laurence Côté, Brielle Demuth, Louise Ghesquière, Emmanuel Bujold
{"title":"<ArticleTitle xmlns:ns0=\"http://www.w3.org/1998/Math/MathML\">Aspirin before the 11 <ns0:math> <ns0:msup><ns0:mrow><ns0:mi> </ns0:mi></ns0:mrow> <ns0:mtext>th</ns0:mtext></ns0:msup> </ns0:math> week of pregnancy to prevent pre-eclampsia.","authors":"Marie-Laurence Côté, Brielle Demuth, Louise Ghesquière, Emmanuel Bujold","doi":"10.18502/ijrm.v22i8.17245","DOIUrl":"10.18502/ijrm.v22i8.17245","url":null,"abstract":"","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 8","pages":"679-680"},"PeriodicalIF":1.6,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study. 在多囊卵巢综合征大鼠模型中,一氧化碳通过调节血红素加氧酶系统改善卵巢结构变化并减轻氧化应激:一项实验研究。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-10-14 eCollection Date: 2024-08-01 DOI: 10.18502/ijrm.v22i8.17231
Bahareh Asadi, Kamran Rakhshan, Mina Ranjbaran, Arash Abdi, Maryam Vaziripour, Behjat Seifi
{"title":"Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study.","authors":"Bahareh Asadi, Kamran Rakhshan, Mina Ranjbaran, Arash Abdi, Maryam Vaziripour, Behjat Seifi","doi":"10.18502/ijrm.v22i8.17231","DOIUrl":"10.18502/ijrm.v22i8.17231","url":null,"abstract":"<p><strong>Background: </strong>Carbon monoxide (CO), influences ovarian function, pregnancy, and placental health. Heme oxygenase (HO)-1 and its products, including CO, exhibit protective and anti-inflammatory properties.</p><p><strong>Objective: </strong>This study investigates the protective effects of CO released by the carbon dioxide-releasing molecule (CORM)-2 against oxidative stress, functional and structural changes of the ovaries, and HO-1 expressions in female rats suffering from polycystic ovary syndrome (PCOS).</p><p><strong>Materials and methods: </strong>In this experimental study, 24 <i>Rattus norvegicus var. Albinus</i> female rats (180-200 gr, 8 wk) were randomly divided into 4 groups (n = 6/each): control, CORM-2 (10 mg/kg), PCOS (induced by 4 mg/kg, intramuscular injection and a single dose of estradiol valerate), PCOS + CORM-2. Ovary histological changes were evaluated by crystal violet staining. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity of ovarian tissue were assessed using enzyme-linked immunosorbent assay. HO-1 expression was evaluated using Western blot.</p><p><strong>Results: </strong>Corpus luteal formation significantly decreased in the PCOS group and was significantly restored with CORM-2 administration compared to the control group (p <math><mo><</mo></math> 0.05). The expression of ovarian HO-1 protein was reduced in the PCOS group compared to controls (p <math><mo><</mo></math> 0.01), and administration of CORM in PCOS rats significantly increased its expression (p <math><mo><</mo></math> 0.0001). In addition, CORM administration markedly reduced ovarian MDA levels and restored SOD activity (p <math><mo><</mo></math> 0.0001).</p><p><strong>Conclusion: </strong>CORM-2 administration to PCOS rats created protective effects by reducing oxidative stress (reducing MDA level and restoring SOD activity) and increasing ovarian HO-1 protein.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 8","pages":"627-638"},"PeriodicalIF":1.6,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of combination of melatonin and L-carnitine on in vitro maturation in mouse oocytes: An experimental study. 褪黑素和左旋肉碱联合使用对小鼠卵母细胞体外成熟的影响:一项实验研究
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16961
Raziye Chegini, Morteza Sadeghi, Sadegh Shirian, Fatemeh Sabbaghziarani, Ehsan Aali, Pouriya Soleimani, Mohammad Reza Ashtari Majelan, Fariba Zafari, Shahram Darabi
{"title":"Effects of combination of melatonin and L-carnitine on in vitro maturation in mouse oocytes: An experimental study.","authors":"Raziye Chegini, Morteza Sadeghi, Sadegh Shirian, Fatemeh Sabbaghziarani, Ehsan Aali, Pouriya Soleimani, Mohammad Reza Ashtari Majelan, Fariba Zafari, Shahram Darabi","doi":"10.18502/ijrm.v22i7.16961","DOIUrl":"10.18502/ijrm.v22i7.16961","url":null,"abstract":"<p><strong>Background: </strong>Melatonin and L-carnitine are free radical scavengers with antiapoptotic and antioxidant properties that improve oocyte development.</p><p><strong>Objective: </strong>This study aimed to find the possible effect of combining 2 antioxidant agents of melatonin and L-carnitine on oocyte morphology, maturation, apoptosis, and expression of bone morphogenetic protein 15 (<i>BMP-15</i>) and growth differentiation factor 9 (<i>GDF-9</i>) genes in a mice model.</p><p><strong>Materials and methods: </strong>To overstimulation, 60 female NMRI mice were injected intraperitoneally using mare serum gonadotropin. On day 2 post-injection, 70 cumulus-oocyte complexes were collected from each mouse. The collected oocytes randomly were then divided into 4 groups including, the control, melatonin, L-carnitine, and melatonin + L-carnitine groups. The morphology and maturation rate of the oocytes was evaluated using a light microscope. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and the expression of <i>BMP-15</i> and growth and differentiation factor <i>GDF-9</i> genes was also evaluated by real-time polymerase chain reaction.</p><p><strong>Results: </strong>Oocyte diameter significantly was increased in combination treatment of L-carnitine and melatonin compared to other groups (p <math><mo><</mo></math> 0.05). L-carnitine group showed the highest mean percentage of oocyte cytoplasmic pattern. Results of the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling indicated that the lowest apoptosis rate belonged to the melatonin + L-carnitine group. Moreover, the combination groups showed the highest number of oocytes and maturation rate. The <i>BMP-15</i> and <i>GDF-9</i> genes were significantly upregulated in all treatment groups compared to the control group.</p><p><strong>Conclusion: </strong>Our results suggested a combination of melatonin + L-carnitine administration as a more effective choice for in vitro promotion of oocyte maturation.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"527-538"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comments on "Negative effect of varicocele on sperm mitochondrial dysfunction: A cross-sectional study". 关于 "精索静脉曲张对精子线粒体功能障碍的负面影响:一项横断面研究
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16976
Elaheh Sanjari, Hadi Raeisi Shahraki
{"title":"Comments on \"Negative effect of varicocele on sperm mitochondrial dysfunction: A cross-sectional study\".","authors":"Elaheh Sanjari, Hadi Raeisi Shahraki","doi":"10.18502/ijrm.v22i7.16976","DOIUrl":"10.18502/ijrm.v22i7.16976","url":null,"abstract":"","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"605-608"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study. FSHR和LHCGR基因多态性对采用全部冻结策略的促性腺激素释放激素拮抗剂方案临床结果的意义:病例对照研究。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16962
Jayesh Amin, Naga Sandhya Alle, Ami Patel, Bansi Prajapathi, Paresh Makwana, Jaya Prakash, Kota Murali Krishna
{"title":"Significance of <i>FSHR</i> and <i>LHCGR</i> gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study.","authors":"Jayesh Amin, Naga Sandhya Alle, Ami Patel, Bansi Prajapathi, Paresh Makwana, Jaya Prakash, Kota Murali Krishna","doi":"10.18502/ijrm.v22i7.16962","DOIUrl":"10.18502/ijrm.v22i7.16962","url":null,"abstract":"<p><strong>Background: </strong>Follicle-stimulating hormone receptor (<i>FSHR</i>) and luteinizing hormone/choriogonadotropin receptor (<i>LHCGR</i>) are integral to ovarian function, facilitating follicle development and maturation through their respective hormonal interactions. The influence of receptor polymorphisms on the outcomes of freeze-all cycles remains unclear.</p><p><strong>Objective: </strong>This study investigates the impact of <i>FSHR</i> <i>N680S</i> and <i>LHCGR</i> <i>N312S</i> polymorphisms on clinical outcomes in freeze-all cycles.</p><p><strong>Materials and methods: </strong>Women undergoing controlled ovarian stimulation for assisted reproductive technology participated in this study. They were administered a gonadotropin-releasing hormone antagonist protocol, with recombinant follicle-stimulating hormone (rFSH) dosages adjusted according to age, body mass index, antral follicle count, and individual hormonal responses. Additionally, human menopausal gonadotropin dosages were tailored based on the <i>LHCGR</i> <i>N312S</i> genetic variant.</p><p><strong>Results: </strong>Analysis revealed no significant differences in age, body mass index, antral follicle count, or marital status across the genotypes of <i>FSHR</i> <i>N680S</i> and <i>LHCGR</i> <i>N312S</i>. However, notable differences were observed in the rFSH dosage required daily and in total among the FSHR polymorphism genotypes. Genotypes of the <i>LHCGR</i> polymorphism correlated with fewer stimulation days. A significant interaction was observed between the 2 polymorphisms concerning total rFSH dosage.</p><p><strong>Conclusion: </strong>The presence of serine in the <i>FSHR</i> polymorphism was associated with higher rFSH dosage requirements. Both <i>FSHR</i> <i>N680S</i> and <i>LHCGR</i> <i>N312S</i> polymorphisms significantly influenced clinical pregnancy and live birth outcomes in freeze-all cycles, underscoring the potential of a pharmacogenomic approach to optimize hormone supplementation in controlled ovarian stimulation protocols during assisted reproductive technology treatments.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"539-552"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of chronic opioid on cognitive function and spermatogenesis in rat: An experimental study. 慢性阿片类药物对大鼠认知功能和精子发生的影响:一项实验研究
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16971
Hamid Norioun, Seyed Jamal Moshtaghian, Firoozeh Alavian, Maryam Khombi Shooshtari, Golnaz Alipour, Saeedeh Ghiasvand
{"title":"Impact of chronic opioid on cognitive function and spermatogenesis in rat: An experimental study.","authors":"Hamid Norioun, Seyed Jamal Moshtaghian, Firoozeh Alavian, Maryam Khombi Shooshtari, Golnaz Alipour, Saeedeh Ghiasvand","doi":"10.18502/ijrm.v22i7.16971","DOIUrl":"10.18502/ijrm.v22i7.16971","url":null,"abstract":"<p><strong>Background: </strong>Opioid analgesics like morphine and methadone are widely used for managing severe pain; however, concerns over their potential misuse and adverse effects on the brain and reproductive system are significant.</p><p><strong>Objective: </strong>We aimed to investigate their impacts on spermatogenesis and cognitive function in male Norway rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 36 male Norway rats (250-300 gr, 6 months old) were divided into 6 groups: low-dose morphine, high-dose morphine, low-dose methadone, high-dose methadone, positive control (received normal saline at 5 mg/kg), and negative control (received no treatment). Morphine and methadone were administered intraperitoneally over 30 days at doses of 3 mg/kg and 7 mg/kg, respectively. Behavioral assessments evaluated anxiety, stress, and short- and long-term memory. Sperm parameters (viability, motility, morphology), hormonal analysis (testosterone, luteinizing hormone, follicle-stimulating hormone, estradiol), and gene expressions (<i>Tp53, CatSper1</i>) were assessed.</p><p><strong>Results: </strong>A significant reduction in rat weight was observed in the high-dose morphine group (p = 0.0045), while testicular weights remained unchanged. Sperm abnormalities were observed with high doses of methadone and morphine. High-dose methadone significantly reduced offspring count (p = 0.0004). Levels of follicle-stimulating hormone, luteinizing hormone, testosterone, and estradiol varied significantly across treatment groups. Gene expression was altered in response to treatments (p <math><mo><</mo></math> 0.05).</p><p><strong>Conclusion: </strong>Prolonged exposure to methadone and morphine resulted in memory dysfunction, chronic stress, hormonal disturbances, altered gene expression, and fertility complications. These effects were more pronounced at higher doses, highlighting the importance of careful dosage management in opioid therapy.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"579-592"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of progesterone and estrogen receptors in treatment choice after endometriosis surgery: A cross-sectional study. 孕激素和雌激素受体在子宫内膜异位症术后治疗选择中的作用:一项横断面研究。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16970
Tahereh Poordast, Saeed Alborzi, Ziba Kiani, Navid Omidifar, Elham Askary, Kefayat Chamanara, Mansoureh Shokripour, Alimohammad Keshtvarz Hesam Abadi
{"title":"The role of progesterone and estrogen receptors in treatment choice after endometriosis surgery: A cross-sectional study.","authors":"Tahereh Poordast, Saeed Alborzi, Ziba Kiani, Navid Omidifar, Elham Askary, Kefayat Chamanara, Mansoureh Shokripour, Alimohammad Keshtvarz Hesam Abadi","doi":"10.18502/ijrm.v22i7.16970","DOIUrl":"10.18502/ijrm.v22i7.16970","url":null,"abstract":"<p><strong>Background: </strong>The lack of improvement in some endometriotic people's pain after surgery even while using hormone treatment may suggest an inappropriate response to routine hormonal therapies.</p><p><strong>Objective: </strong>This study aimed to determine a cut-off point for selecting the most appropriate treatment based on the hormone receptors of endometriotic lesions.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, by reviewing the medical records of participants and testing their archive samples and phone interviews (if needed), 86 symptomatic women after endometriosis surgery who were operated into governmental hospitals, Shahid Faghihi and Hazrate Zeinab Shiraz Iran were enrolled between March 2017 and March 2019. Women were divided into 2 groups: responsiveness (n = 73 for dysmenorrhea, n = 60 for dyspareunia) to medical treatment and surgery, and unresponsiveness (n = 13, n = 7). We examined the pathological slides of 86 women to determine the amount of hormone receptors and the relationship between the type of medical treatment and the level of hormone receptors on pain relief within 1 yr after surgery.</p><p><strong>Results: </strong>Based on the receiver operating characteristic curve, dysmenorrhea in the presence of tissue estrogen receptors <math><mo>></mo></math> 60% (p = 0.1065), and dyspareunia in the presence of tissue progesterone receptors <math><mo>></mo></math> 80% (p = 0.0001) responded well to medical treatment after surgery. In the presence of endometrioma-dysmenorrhea showed the best response to oral contraceptive pills (69.4%), while in deep infiltrative endometriosis-dyspareunia showed the best response to progesterone treatment (75%).</p><p><strong>Conclusion: </strong>Prescribing an appropriate hormone therapy based on a specific immunohistochemistry staining pattern can improve the life quality of postoperative endometriosis individuals.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"567-578"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Melatonin's protective effect against placental transfer of Methadone in mice: An experimental study. 褪黑素对小鼠胎盘转移美沙酮的保护作用:一项实验研究。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16957
Maryam Akbarzadeh, Ramin Ataee, Farkhondeh Nemati, Abbas Ali Dehpouri, Fatemeh Shaki
{"title":"Melatonin's protective effect against placental transfer of Methadone in mice: An experimental study.","authors":"Maryam Akbarzadeh, Ramin Ataee, Farkhondeh Nemati, Abbas Ali Dehpouri, Fatemeh Shaki","doi":"10.18502/ijrm.v22i7.16957","DOIUrl":"10.18502/ijrm.v22i7.16957","url":null,"abstract":"<p><strong>Background: </strong>Methadone is a substance widely used in the substitution treatment of opiate addiction in pregnancy. The placental transfer of methadone influences oxidative stress processes. Melatonin is a hormone with antioxidant activity.</p><p><strong>Objective: </strong>This study aimed to evaluate the protective effects of melatonin on oxidative stress induced by the transfer of transplacental methadone in mice.</p><p><strong>Materials and methods: </strong>In this experimental study, 36 female mice (2 months old, 20 <math><mo>±</mo></math> 2 gr) were divided into 6 groups (n = 6/each) of control, methadone (0.3 mg/kg intraperitoneal, single dose) and melatonin (2, 4, and 6 mg/kg/day gavage) were administered 30 min before methadone, and one group received melatonin alone (0.6 mg/kg with single injection). Administration for 10 consecutive days of the pregnancy period was done. After baby mice were born, all neonatal mice were killed by beheading or sacrificing after anesthesia. The liver tissues were extracted. The samples were then sent for studying oxidative stress markers such as lipid peroxidation, glutathione, and protein carbonyl contents. Also, we have used the immunohistochemistry method for apoptotic markers such as: BAX, Bcl2, and Caspase3 for assaying apoptosis.</p><p><strong>Results: </strong>This study has shown that methadone caused a significant decrease in glutathione concentration (p = 0.035). Also, we observed a significant increase in lipid peroxidation and protein carbonyl contents (p = 0.015, 0.025 respectively). However, melatonin treatment significantly inhibited oxidative stress markers (p = 0.025). Also, apoptosis assay has shown that melatonin could decrease BAX and Caspase 9 as apoptotic and increase Bcl2 as an antiapoptotic proteins (p = 0.015).</p><p><strong>Conclusion: </strong>Our findings have shown that melatonin has a protective effect against oxidative stress and apoptosis induced by the placental transfer of methadone via its antioxidant effects.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"515-526"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the natural killer cell subsets and their relationship with serum interferon gamma and vitamin D levels in women with stages III and IV endometriosis: A case-control study. 评估 III 期和 IV 期子宫内膜异位症妇女的自然杀伤细胞亚群及其与血清干扰素 γ 和维生素 D 水平的关系:病例对照研究。
IF 1.6
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI: 10.18502/ijrm.v22i7.16933
Samira Najafi Chamgordani, Nafiseh Esmaeil, Maryam Hashemi, Afshin Amari, Maryam Seyedtabib, Mehri Ghafourian
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