International Journal of Reproductive Biomedicine最新文献

{"title":"Prevalence of psychological symptoms in low and high-risk pregnant women: A cross-sectional study.","authors":"Maryam Dalili, Ali Mehdizadeh, Maryam Nodinnejad, Fatemeh Karami Robati","doi":"10.18502/ijrm.v23i4.18781","DOIUrl":"10.18502/ijrm.v23i4.18781","url":null,"abstract":"<p><strong>Background: </strong>Common psychological disorders during pregnancy can have obvious harmful effects on both mother and fetus.</p><p><strong>Objective: </strong>This study aimed to investigate the prevalence of psychological symptoms in low and high-risk pregnant women.</p><p><strong>Materials and methods: </strong>This cross-sectional study was conducted on 400 low-risk and high-risk pregnant women in Afzalipour hospital, Kerman, Iran from December 2017-2018. Participants were selected by census method, and the data collection tool was a 90-item questionnaire named Symptom Checklist-90.</p><p><strong>Results: </strong>The mean age of pregnant women was 29.1 <math><mo>±</mo></math> 6.8 yr. 7.6% had gestational diabetes mellitus, 5.9% had pregnancy hypertension, 6.6% had a history of in vitro fertilization, and 17.5% had a history of one miscarriage. 61.2 and 65.5% of high-risk women had depression and anxiety, respectively. A significant difference was observed between low-risk and high-risk women in terms of depression (p = 0.019), anxiety (p = 0.049), and aggression (p = 0.013), and the frequency of these variables was higher in high-risk women than in low-risk women.</p><p><strong>Conclusion: </strong>According to age, education, and gestational period, the differences between 2 groups (low-risk and high-risk) were significant. Compared with low-risk women, high-risk pregnant women reported a higher prevalence of psychological symptoms in 10 factors. High-risk pregnant women had a significantly higher prevalence of somatization symptoms, obsessive-compulsive symptoms, depression symptoms, anxiety symptoms, hostility symptoms, and paranoid ideation than low-risk women. Therefore, educational programs during pregnancy for high-risk women can be useful.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 4","pages":"295-302"},"PeriodicalIF":1.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of adding autologous platelet-rich plasma to embryo transfer media on implantation outcomes: An RCT.","authors":"Marzie Sanuie Farimani, Ashraf Aleyasin, Ashraf Moini, Iraj Amiri, Jalal Poorolajal, Fahime Salari Shahrbabaki","doi":"10.18502/ijrm.v23i4.18785","DOIUrl":"10.18502/ijrm.v23i4.18785","url":null,"abstract":"<p><strong>Background: </strong>Several recent studies have shown the beneficial effect of platelet-rich plasma (PRP) in improving endometrial function in cases with repeated implantation failure and thin endometrium. However, the mechanism of this effect is unclear.</p><p><strong>Objective: </strong>To investigate the direct effect of PRP on the implantation process during embryo transfer (ET) in cases without an obvious history of abnormality on implantation.</p><p><strong>Materials and methods: </strong>In this randomized clinical trial study, 55 infertile women (20-40 yr) who were candidates for intracytoplasmic sperm injection referred to the Infertility Center of Fatemiyeh hospital in Hamadan, Iran between September 2022 and 2023were randomly divided into intervention and control groups. In the intervention group, PRP was added to the ET medium, while in the control group, a routine ET medium was used. After ET, chemical and clinical pregnancy were measured.</p><p><strong>Results: </strong>In the intervention and control groups beta human chorionic gonadotropin positivity 14 days after transfer was 33.3% and 39.3%, consecutively (p = 0.646). On day 21 post-transfer, a gestational sac was observed in 33.3% and 35.7% of cases (p = 0.646). By 28 days after transfer fetal heart activity was detected in 33.3% and 35.7% of cases (p = 0.631).</p><p><strong>Conclusion: </strong>The addition of autologous PRP at the time of ET does not have a direct positive effect on embryo implantation, and the observation of positive effects in previous studies may be indirectly on the uterine microenvironment, which requires time.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 4","pages":"333-342"},"PeriodicalIF":1.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effects of melatonin on testis, sperm parameters quality, and in-vitro fertilization in mice following treatment with aflatoxin B1: An experimental study.","authors":"Maryam Sabahi, Mojtaba Karimipour, Abass Ahmadi, Bagher Pourheydar, Gholamhossein Farjah","doi":"10.18502/ijrm.v23i2.18492","DOIUrl":"10.18502/ijrm.v23i2.18492","url":null,"abstract":"<p><strong>Background: </strong>Aflatoxin B1 (AFB1) contamination of foods and animal feeds is a public health issue. Exposure to AFB1 induces oxidative stress and can cause male reproductive toxicity. Melatonin (MLT) is a neuro-hormone produced by the pineal gland and the testis and is known as a potent antioxidant.</p><p><strong>Objective: </strong>This study aims to determine the protective effect of MLT on testicular tissue alterations, sperm parameter indexes, and in vitro fertility assays in mice treated with AFB1.</p><p><strong>Materials and methods: </strong>In this experimental study, 28 adult male NMRI mice (8-10 wk old, 25-27 gr) were divided randomly into 4 groups: control, MLT (20 mg/kg/day, intraperitoneally), AFB1 (50 <math><mi>μ</mi></math> g/kg/day, intraperitoneally) and MLT+AFB1. After 35 consecutive days, testis histological changes, sperm quality parameters, the rate of sperm with DNA damage, and in vitro fertilization outcomes up to the blastocyst stage were surveyed and compared between groups.</p><p><strong>Results: </strong>Our results showed that AFB1 administration induced histological alterations in the testis and significantly decreased all the sperm parameters and in vitro fertility (fertilization and blastocyst formation rates) compared to control. Additionally, the percentages of immature sperms and sperms with DNA damage significantly (p <math><mo><</mo></math> 0.001) increased in the AFB1-treated group. MLT treatment in the MLT+AFB1group significantly increased testis quality and sperm parameters and improved in vitro fertilizaton rate and in vitro embryonic development.</p><p><strong>Conclusion: </strong>These findings demonstrated that MLT can compensate for the adverse effects of AFB1 on the quality of testicular tissue, sperm parameters, sperm DNA, and in vitro fertilization outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"185-198"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic potential of cell-free fetal nucleic acids in predicting pregnancy complications: A systematic review and meta-analysis on trisomy, pre-eclampsia, and gestational diabetes.","authors":"Jiut Ram Keshari, Pritam Prakash, Seema Rani Sinha, Prem Prakash, Kirti Rani, Tarique Aziz, Shaily Shilpa","doi":"10.18502/ijrm.v23i2.18476","DOIUrl":"10.18502/ijrm.v23i2.18476","url":null,"abstract":"<p><strong>Background: </strong>Recent studies reveal an association between increased cell-free fetal (cff) nucleic acid in maternal blood and pregnancy challenges like loss, pre-eclampsia, growth restriction, and preterm labor.</p><p><strong>Objective: </strong>This article assesses the role of cff nucleic acids as potential diagnostic markers for the prediction and monitoring progression of severe pregnancy-related complications.</p><p><strong>Materials and methods: </strong>In this systematic review and meta-analysis, various databases were searched. Original articles reporting on the role of cff nucleic acids in predicting the complications of pregnancy were included. I square test and funnel plot were used to analyze heterogeneity and publication bias, respectively. The quality of studies was assessed using the critical appraisal checklists for studies created by the Joanna Briggs Institute.</p><p><strong>Results: </strong>70 publications were selected for the final qualitative analysis. Articles were published between 2010 and 2023, and most studies were conducted in the USA and China. The majority of studies were conducted on the quantity of cff-DNA (n = 40), and the remaining on microRNA (n = 18), messenger RNA (n = 11), and cell-free RNA (n = 1). The pooled sensitivity of cff nucleic acids for detecting trisomy was found to be 90.9 (95% CI: 80.9-100%). MicroRNA levels were significantly increased in participants with gestational diabetes mellitus, with a standardized mean difference of 1.22 (95% CI: -0.90-3.34).</p><p><strong>Conclusion: </strong>Fetal nucleic acids can serve as accurate noninvasive diagnostic tools for predicting serious complications during pregnancy.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"111-130"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometrial compaction can improve assisted reproductive technology outcomes in frozen-thawed embryo transfer cycles using hormone replacement therapy: A cross-sectional study.","authors":"Shahrzad Moeinaddini, Saeideh Dashti, Zahra Amini Majomerd, Nooshin Hatamizadeh","doi":"10.18502/ijrm.v23i2.18484","DOIUrl":"10.18502/ijrm.v23i2.18484","url":null,"abstract":"<p><strong>Background: </strong>Endometrial compaction (EC) is an ultrasound evaluation method that may predict assisted reproductive technology outcomes.</p><p><strong>Objective: </strong>This study aimed to assess the impact of EC on assisted reproductive technology outcomes in frozen embryo transfer cycles with hormone replacement therapy.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, 100 women who underwent first or second frozen embryo transfer cycle at Yazd Reproductive Sciences Institute, Yazd, Iran from June to October 2024 were included. Endometrial thickness was compared between the day of starting progesterone and embryo transfer day. Then participants were divided into 2 groups, no compaction and compaction group. Biochemical, clinical, and ongoing pregnancy rates (OPR) were assessed between the 2 groups.</p><p><strong>Results: </strong>Statistically significant differences were observed in biochemical, clinical, and OPR between the compaction and no compaction groups. Logistic regression analysis demonstrated significantly higher pregnancy rates in EC 10-15% and <math><mo>></mo></math> 15%. We found a significant influence of EC 10-15% (p = 0.02, p = 0.01, p = 0.01), and EC <math><mo>></mo></math> 15% (p = 0.002, p = 0.001, and p = 0.002) on biochemical, clinical, and OPR, respectively.</p><p><strong>Conclusion: </strong>EC after progesterone administration in hormone replacement therapy-frozen embryo transfer cycles can increase biochemical, clinical, and OPR. The percentage of EC changes also influence the outcomes of these cycles.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"141-152"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An investigation of the therapeutic potential of the testicular tissue encapsulated in amnion membrane in mouse model: An experimental study.","authors":"Keykavoos Gholami, Elahe Asheghmadine, Fateme Guitynavard, Leila Zareian Baghdadabad, Diana Taheri, Parisa Zahmatkesh, Leonardo Oliveira Reis, Seyed Mohammad Kazem Aghamir","doi":"10.18502/ijrm.v23i2.18489","DOIUrl":"10.18502/ijrm.v23i2.18489","url":null,"abstract":"<p><strong>Background: </strong>Restoring fertility in male cancer individuals through testicular tissue transplantation faces challenges due to hypoxia-induced loss of spermatogonial stem cells (SSCs). Hydrogel encapsulation was explored to minimize hypoxic damage in testicular tissue transplantation. For this purpose, human amnion membrane (hAM)-derived hydrogel could be an alternative.</p><p><strong>Objective: </strong>The potential of hAM-derived hydrogel to support testis tissue grafts was evaluated.</p><p><strong>Materials and methods: </strong>In this experimental study, testicular tissue samples (1-3 mm³) were obtained from 16 male NMRI mice (4-5 wk, 22 <math><mo>±</mo></math> 2 gr). These tissue fragments were either encapsulated within a hydrogel derived from a hAM or left unencapsulated (control) prior to being autologously transplanted beneath the dorsal skin of mice subjected to hemilateral or bilateral orchiectomy. The grafted testicular tissues were histologically evaluated for key parameters, including the integrity of seminiferous tubules, survival of SSCs, Sertoli cell functionality, as well as hypoxia and apoptosis on day 21.</p><p><strong>Results: </strong>No significant differences were observed between groups regarding ST integrity, number of SSCs, Sertoli cell functionality, or the rate of hypoxia-inducible factor 1-alpha and apoptosis (p <math><mo>≤</mo></math> 0.05).</p><p><strong>Conclusion: </strong>In conclusion, this study demonstrated no effect of hAM hydrogel encapsulation on the outcomes of testicular tissue transplantation.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"171-184"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of laparoscopic surgery effects on pain severity and quality of life in different subtypes of endometriosis: A follow-up study.","authors":"Mania Kaveh, Haniye Malakouti, Shahla Chaichian, Abolfazl Mehdizadeh Kashi, Mahdi Afshari, Kambiz Sadegi","doi":"10.18502/ijrm.v23i2.18494","DOIUrl":"10.18502/ijrm.v23i2.18494","url":null,"abstract":"<p><strong>Background: </strong>Studies have shown that endometriosis significantly has a negative impact on women's mental health and quality of life (QoL), resulting in these participants experiencing a diminished QoL.</p><p><strong>Objective: </strong>This study aimed to evaluate the effect of laparoscopic surgery on the severity of pain and the QoL of women with different types of endometriosis.</p><p><strong>Materials and methods: </strong>In this follow-up study 50 women with endometriosis, who underwent laparoscopic surgery, who met a high visual analog score ( <math><mo>></mo></math> 6) with impaired QoL, lack of response to analgesics, infertility with pain unresponsive to assisted reproductive therapy, and involvement of other organs such as the bowel or ureter at the Amir Al Momenin hospital, Zabol, Iran, from August 2022 to January 2023 were enrolled. The participants were categorized into 2 groups: those with stage IV endometriosis and those with lower stages (I-III). Groups according to the higher occurrence of grade 4 endometriosis, as 55.1% were affected by it. The pain score was measured using the visual analog score, and the participants' QoL score was measured using the endometriosis health profile questionnaire.</p><p><strong>Results: </strong>The mean age of participants was 32 yr, with a standard deviation of 8.6 yr. Participants were divided into 2 groups: those with grade 4 endometriosis (55.1%) and those with a grade lower than 4, as classified by the American Society for Reproductive Medicine. Over 12 months, both groups experienced a significant decrease in pain severity and QoL scores (p <math><mo><</mo></math> 0.0001). However, the changes in pain and QoL scores between the 2 groups were not statistically significant (p = 0.520 and p = 0.984, respectively).</p><p><strong>Conclusion: </strong>Laparoscopic treatment can reduce pain and QoL scores (increase the QoL indices) in women with endometriosis, regardless of the disease's severity.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"199-206"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ovarian hyperstimulation syndrome on intracytoplasmic sperm injection outcomes in poly-cystic ovarian syndrome women: A cross-sectional study.","authors":"Mufeda Ali Jwad, Maryam Hussein Khaleefah, Ramih Abd AlFattah Naser","doi":"10.18502/ijrm.v23i2.18496","DOIUrl":"10.18502/ijrm.v23i2.18496","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovarian syndrome (PCOS) is a complex disorder that affects the endocrine, metabolic, and reproductive systems. While controlled ovarian hyperstimulation can increase the number of oocytes retrieved and improve the number of good-quality embryos, it may also negatively affect oocyte maturity, embryo quality, endometrial receptivity, and pregnancy outcomes.</p><p><strong>Objective: </strong>This study aimed to find out if ovarian hyperstimulation syndrome (OHSS) has negative effects on intracytoplasmic sperm injection outcomes in a sample of PCOS women.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, data of 84 PCOS women who underwent controlled ovarian stimulation using a flexible antagonist protocol-treated intracytoplasmic sperm injection cycles referred to the Infertility Department of Higher Institute for the Diagnosis of Infertility and Assisted Reproduction Techniques, Baghdad, Iraq between January 2020 and December 2023 was extracted from their medical records. Participants were categorized into 2 groups after undergoing controlled ovarian hyperstimulation protocol: PCOS with OHSS (n = 54) and PCOS without OHSS (n = 30). The dosage was tailored based on age, body mass index, and response to previous stimulation cycles, if applicable. The trigger was administered using decapeptyl 0.2 mg alone or in combination with recombinant human chorionic gonadotrophin, depending on the estradiol levels at the time of the trigger.</p><p><strong>Results: </strong>Significant differences were observed in the duration of stimulation (p = 0.005), oocyte yield (p = 0.001), mature oocytes (p = 0.001), and fertilized oocytes (p = 0.036); however, no significant difference was observed, neither in number of good quality embryos (p = 0.52) nor in pregnancy rate (p = 0.54) after fresh and frozen embryo transfer between the 2 groups.</p><p><strong>Conclusion: </strong>OHSS does not affect the embryo quality or pregnancy rate in PCOS women.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"207-216"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetic pregnancy: A literature review of maternal and neonatal adverse outcomes.","authors":"Sara Mohamed, Waverly Kundysek, Niraj Vora, Vinayak Govande, Raza Bajwa, Mohammad Nasir Uddin","doi":"10.18502/ijrm.v23i2.18482","DOIUrl":"10.18502/ijrm.v23i2.18482","url":null,"abstract":"<p><p>Of all pregnant women in the United States an average of 1.5% reported to have type 1 or type 2 diabetes mellitus. Our review article will discuss and explore the relationship between pre-pregnancy diabetes and its adverse outcomes in mothers and neonates. Diabetes in pregnancy can cause a myriad of complications, many of which are related to microvascular changes, including diabetic nephropathy and retinopathy associated with preterm delivery, cesarean sections, and intrauterine growth restriction. Pregnant patients with diabetes also have an increased risk of pre-eclampsia, likely due to complications related to abnormal structure and function of the placenta. In addition, cardiovascular complications are more common and may present antepartum, intrapartum, or postpartum. Adverse neonatal outcomes that have been observed in diabetic pregnancies include fetal stillbirth and perinatal death, macrosomia, congenital malformations, respiratory distress, and neurological impairments. These complications explain the increased morbidity and mortality rate of infants of diabetic mothers, and the increased frequency of neonatal intensive care unit hospitalizations after birth. Diabetes in pregnancy causes a spectrum of changes in the maternal-fetal interface. This review addresses the placental changes during pregnancy and its adverse maternal and neonatal outcomes. We strongly believe the material discussed in this article can help in understanding the effects of diabetes during pregnancy which will ultimately aid in designing interventions to prevent these adverse outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"131-140"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of addiction behavior and spermatogenesis in glial cell line-derived neurotrophic factor-treated cannabis-addicted rats: An experimental study.","authors":"Rozhina Laleh, Mitra Heydari Nasrabadi, Parvin Khodarahmi, Jamshid Soltani","doi":"10.18502/ijrm.v23i2.18487","DOIUrl":"10.18502/ijrm.v23i2.18487","url":null,"abstract":"<p><strong>Background: </strong>Cannabis addiction poses risks to male fertility by lowering levels of glial cell line-derived neurotrophic factor (GDNF), which is vital for spermatogenesis.</p><p><strong>Objective: </strong>This research aimed to determine if the injection of exogenous GDNF into the brain of cannabis-addicted rats has a positive impact on their behavior and spermatogenesis.</p><p><strong>Materials and methods: </strong>This study involved 15 male Wistar rats divided into 3 equal groups: control, model, and experimental. Cannabis addiction was induced in the model and experimental groups using a smoking machine with a 0.25 gr dose per 5 rats, and the experimental group received a 0.5 mg GDNF treatment via stereotaxic injection. Behavioral changes were assessed through plus maze, open field, and sucrose preference tests before and after treatments. Sperm parameters were evaluated with H&E staining, sperm morphology with Diff-Quik staining, DNA damage and viability with acridine orange and trypan blue staining.</p><p><strong>Results: </strong>Addicted rats displayed increased anxiety, which was improved by GDNF treatment (p <math><mo><</mo></math> 0.05). Although cannabis significantly reduced germ cells and the size of the testis and epididymis compared to controls (p = 0.0006, p = 0.003), GDNF had a limited effect on these aspects. Cannabis significantly altered sperm morphology (p = 0.0016), but GDNF reversed abnormal sperms. GDNF improves sperm quality, reverses cannabis-induced sperm grading alterations (grade C, p = 0.0295), reduces DNA damage significantly (p = 0.0242), and enhances sperm viability, highlighting its potential to counteract some of cannabis's harmful effects on male reproductive health.</p><p><strong>Conclusion: </strong>The findings of this experiment suggest that exogenous GDNF could be a potential therapeutic agent for cannabis addiction and sperm parameters.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 2","pages":"153-170"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}