International Journal of Reproductive Biomedicine最新文献

{"title":"Closer look at circulating nitric oxide levels and their association with polycystic ovary syndrome: A meta-analytical exploration.","authors":"Seyed Sobhan Bahreiny, Akram Ahangarpour, Elnaz Harooni, Mahdi Amraei, Mojtaba Aghaei, Reza Mohammadpour Fard","doi":"10.18502/ijrm.v22i12.18061","DOIUrl":"10.18502/ijrm.v22i12.18061","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovary syndrome (PCOS) casts a wide shadow over the reproductive health of millions of women worldwide, emerging as one of the most complex and multifaceted endocrine disorders. In addition, nitric oxide (NO) stands out as a pivotal signaling molecule, orchestrating a symphony of physiological processes.</p><p><strong>Objective: </strong>This meta-analysis aims to elucidate the association between NO levels and PCOS, investigate the potential of NO as a biomarker for PCOS diagnosis, and evaluate its clinical significance.</p><p><strong>Materials and methods: </strong>A systematic review was conducted in several electronic databases, including PubMed, Web of Science, Cochrane Library, Scopus, EMBASE, and Google Scholar, to identify relevant studies published up to January 2024. Standardized mean difference and 95% CI were calculated using a random effects model to assess the overall effect size. Meta-regressions and subgroup analysis were performed to investigate sources of heterogeneity.</p><p><strong>Results: </strong>A meta-analysis of 14 studies with 1171 participants showed that NO levels were significantly lower in the PCOS group than in the control group. The pooled analysis yielded a standardized mean difference of -0.482; 95% CI: -0.908 to -0.056; p = 0.027. Subgroup analyses further demonstrated variations in NO levels between different PCOS phenotypes and in relation to metabolic parameters.</p><p><strong>Conclusion: </strong>This meta-analysis provides evidence for an association between PCOS and dysregulated NO levels and suggests a potential role of NO as a biomarker in the diagnosis and pathogenesis of PCOS.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"943-962"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased expression of <i>miR-200a</i> and <i>miR-223-3p</i> in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility.","authors":"Yasaman Nazari Hagh, Mohamadreza Ahmadifard, Sedigheh Esmaelzadeh, Soheila Abbaszadeh, Naser Shokrzadeh","doi":"10.18502/ijrm.v22i12.18066","DOIUrl":"10.18502/ijrm.v22i12.18066","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis (EM) is a condition that causes infertility with decreasing uterine receptivity. It is reported that it affects about 20-25% of all infertile women. Some genetic markers play a crucial role in pathogenesis and infertility.</p><p><strong>Objective: </strong>This study investigates the role of <i>miR-200a</i> and <i>miR-223-3p</i> in embryo implantation and their association with EM-related infertility.</p><p><strong>Materials and methods: </strong>In this case-control study, 36 women who referred to the Center for Research on Reproductive Health and Infertility of Babol University of Medical Sciences and Fatemeh Al-Zahra Infertility Specialized Treatment Center in Babol, Iran between June 2022 and July 2023 were evaluated. Participants were divided into 2 EM and control groups (n = 18/each). Endometrial samples were collected from participants between 17 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> and 24 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> days of their menstrual cycle. Histopathological examination (hematoxylin and eosin and periodic acid schiff) was performed to confirm the secretory stage, and <i>miR-200a</i> and <i>miR-223-3p</i> expressions were analyzed by quantitative reverse transcriptase-polymerase chain reaction.</p><p><strong>Results: </strong>Histological analysis confirmed that both groups were in the secretory stage. Additionally, miRNA expression results showed a significant decrease in the <i>miR-200a</i> and <i>miR-223-3p</i> expression levels in EM group compared to control group. The expression level of <i>miR-223-3p</i> and <i>miR-200a</i> in the eutopic endometrial tissue of women with EM was notably lower than those in the control group.</p><p><strong>Conclusion: </strong>Our results suggest that <i>miR-200a</i> and <i>miR-223-3p</i> are involved in the EM pathogenesis, while other genes and signaling pathways are probably involved in the implantation failure.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"1003-1014"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of salivary antioxidant level in polycystic ovary syndrome women under assisted reproductive technology treatment: A case-control study.","authors":"Narges Gholizadeh, Maryam Koopaie, Ashraf Aleyasin, Atousa Mortazavi Milani, Marziyeh Aghahosseini, Mohammad Javad Kharrazifard, Mohadeseh Bahmaee","doi":"10.18502/ijrm.v22i12.18063","DOIUrl":"10.18502/ijrm.v22i12.18063","url":null,"abstract":"<p><strong>Background: </strong>Infertility is defined as the inability to conceive after 12 months of regular unprotected intercourse. Approximately 85% of infertile couples have an identifiable cause, one of the most common causes of infertility is polycystic ovary syndrome (PCOS). The reduction of antioxidant levels in serum and follicular fluid in infertile women compared to healthy women shows the importance of further studying these markers.</p><p><strong>Objective: </strong>To study salivary and serum antioxidant levels in PCOS participants under assisted reproductive technology.</p><p><strong>Materials and methods: </strong>This case-control study was conducted on 80 women in 2 groups including normal participants as control and PCOS groups (n = 40/each). Serum and salivary antioxidant levels such as saliva superoxide dismutase (SOD), saliva anti-Müllerian hormone (AMH), serum SOD, serum total oxidant status, and serum AMH were measured.</p><p><strong>Results: </strong>The average age of participants was 31.6 <math><mo>±</mo></math> 5.4 yr. In both the saliva and serum, antioxidant levels differed significantly between the PCOS and control groups. Key findings showed that the PCOS group had different antioxidant levels and higher serum AMH levels compared to the control group, with all differences being statistically significant (p <math><mo><</mo></math> 0.05).</p><p><strong>Conclusion: </strong>Our finding underscored that saliva antioxidant levels, especially SOD, are a good marker for PCOS diagnosis. It is noninvasive, can easily be performed by the participants, and can be collected in various settings without specialized equipment.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"975-984"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of dienogest and gonadotropin-releasing hormone agonist on pelvic pain after laparoscopic surgery for endometriosis: An RCT.","authors":"Fatemeh Davari Tanha, Azam Rasti, Hamideh Pakniat, Shohreh Salimi Setudeh","doi":"10.18502/ijrm.v22i12.18065","DOIUrl":"10.18502/ijrm.v22i12.18065","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is a chronic inflammatory condition associated with debilitating chronic pelvic pain that affects women's quality of life. Several drugs have been used to reduce pain and psychological distress associated with this disease. Currently, gonadotropin-releasing hormone (GnRH) agonists and dienogest are the most widely used medical therapies for endometriosis.</p><p><strong>Objective: </strong>This study aimed to investigate the efficacy of dienogest and GnRH agonists in improving pelvic pain after laparoscopic surgery for endometriosis.</p><p><strong>Materials and methods: </strong>In this randomized clinical trial study, 104 women with endometriosis who were referred to the Department of Reproductive Medicine of Yas hospital, Tehran, Iran, between April 2022 and March 2023 were studied. After laparoscopic surgery, individuals were randomly assigned into 2 groups (n = 52/each): the dienogest-administered group and the GnRH agonist-administered group. Participants were followed up at 3 months and pelvic pain was measured using the visual analog scale. Pelvic pain and adverse effects of drugs were compared between the groups.</p><p><strong>Results: </strong>Pelvic pain significantly improved in both treatment groups (p <math><mo><</mo></math> 0.0001). No significant difference was observed in hot flashes and joint pain between the dienogest and GnRH agonist groups. However, a significant difference was found in vaginal dryness (p = 0.03) and decreased libido (p = 0.02). GnRH agonists and dienogest reduced irregular vaginal bleeding.</p><p><strong>Conclusion: </strong>Our results suggested that the effect of GnRH agonists and dienogest in improving pelvic pain for endometriosis is the same after a 3-month treatment period. However, these 2 drugs caused different adverse effects.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"995-1002"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Impact of chronic opioid on cognitive function and spermatogenesis in rat: An experimental study\" [Int J Reprod BioMed 2024; 22: 579-592].","authors":"Hamid Norioun, Seyed Jamal Moshtaghian, Firoozeh Alavian, Maryam Khombi Shooshtari, Golnaz Alipour, Saeedeh Ghiasvand","doi":"10.18502/ijrm.v22i10.17671","DOIUrl":"https://doi.org/10.18502/ijrm.v22i10.17671","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.18502/ijrm.v22i7.16971.].</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 10","pages":"793-800"},"PeriodicalIF":1.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pregnancy outcome after adding oral or intramuscular progesterone to vaginal progesterone in frozen embryo transfer: A cross-sectional study.","authors":"Sahereh Arabian, Maryam Eftekhar, Saeideh Dashti, Nahid Homayoon, Elham Nikfarjam","doi":"10.18502/ijrm.v22i10.17661","DOIUrl":"10.18502/ijrm.v22i10.17661","url":null,"abstract":"<p><strong>Background: </strong>Currently, frozen embryo transfers (FET) account for 41% of all embryo transfer cycles. Vaginal progesterone preparations have become the leading choice for luteal phase support due to their convenient application; however, using only vaginal progesterone during FET cycles results in a lower ongoing pregnancy rate.</p><p><strong>Objective: </strong>This study aimed to investigate whether replacing intramuscular (IM) progesterone with oral dydrogesterone in FET cycles affects pregnancy outcomes or not.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, pregnancy outcomes were analyzed in women who underwent cleavage stage FET during an endometrial preparation cycle using hormone replacement therapy at Yazd Reproductive Sciences Institute, Yazd, Iran, between April 2023 and November 2023. The study examined 2 groups based on a luteal phase support regimen: the dydrogesterone group, which received vaginal progesterone and oral dydrogesterone, and the IM progesterone group, which received vaginal progesterone and IM progesterone. Data were extracted from patient files to compare outcomes between the 2 groups.</p><p><strong>Results: </strong>A total of 960 cycles meeting the inclusion criteria were analyzed, with 292 women in the dydrogesterone group and 668 women in the IM progesterone group, and pregnancy outcomes were compared between the 2 groups. The chemical pregnancy rates (28.4% vs. 29.9%, p = 0.636), clinical pregnancy rates (25.3% vs. 26.9%, p = 0.604), and ongoing pregnancy rates (21.9% vs. 23.8%, p = 0.525) were lower and miscarriage rates (14.7% vs. 11.7%, p = 0.210) were higher in dydrogesterone group compared to IM progesterone group, although this difference was not statistically significant.</p><p><strong>Conclusion: </strong>Based on the ease of use and similar pregnancy outcomes of oral dydrogesterone, it can potentially replace the daily injections of IM progesterone.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 10","pages":"763-770"},"PeriodicalIF":1.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follicular fluid meiosis activating sterol supplementation enhances oocyte maturation and fertilization in a microfluidic system: A lab trial study.","authors":"Hossein Torkashvand, Ronak Shabani, Tayebe Artimani, Shamim Pilehvari, Mahdi Moghimi, Mehdi Mehdizadeh","doi":"10.18502/ijrm.v22i10.17663","DOIUrl":"10.18502/ijrm.v22i10.17663","url":null,"abstract":"<p><strong>Background: </strong>In vitro maturation (IVM) is a promising technique in assisted reproductive technologies, offering benefits such as reducing the risk of ovarian hyperstimulation syndrome.</p><p><strong>Objective: </strong>This study aimed to evaluate the effects of timed follicular fluid meiosis-activating sterol (FF-MAS) supplementation on the IVM of germinal vesicle oocytes using a dynamic microfluidic system.</p><p><strong>Materials and methods: </strong>In this lab trial study, 266 germinal vesicle oocytes were collected from the Infertility Center of Fatemieh hospital, Hamedan, Iran between June 2023 and January 2024. The oocytes were allocated into 3 groups for dynamic microfluidic culture. Each group received culture medium at a flow rate of 0.36 µL/min for 24 hr through inlet A and FF-MAS supplementation through inlet B for 1, 2, and 6 hr. The study evaluated maturation and fertilization rates, embryo development, and mitochondrial status, which was assessed using the JC-1 mitochondrial membrane potential assay.</p><p><strong>Results: </strong>Maturation rates were significantly higher in the medium-term FF-MAS exposure (MTG) and long-term FF-MAS exposure groups compared to the short-term FF-MAS group (STG) (p <math><mo><</mo></math> 0.05). Fertilization rates were also higher in the MTG and long-term FF-MAS group compared to the STG (p <math><mo><</mo></math> 0.05). Embryo formation rates and the proportion of good-quality embryos were higher in the MTG compared to the STG (100% vs. 75%; p = 0.03) and (83.3% vs. 33.3%; p = 0.01), respectively. Mitochondrial peripheral distribution was significantly higher in the MTG than in the STG (p = 0.04).</p><p><strong>Conclusion: </strong>Optimizing FF-MAS exposure duration enhances IVM efficiency, offering a promising strategy to increase oocyte utilization in in vitro fertilization programs.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 10","pages":"781-792"},"PeriodicalIF":1.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secretome of human amniotic membrane stem cells promote recovery and testicular functions through modulating <i>SIRT1/NRF2/TNF-α</i> pathway in mice testicular torsion: An experimental study.","authors":"Roghayeh Esfehani, Farnaz Khadivi, Jamal Valipour, Maryam Shabani, Mahya Ramesh, Parinaz Javanbakht, Davood Zarini, Sina Mojaverrostami, Masih Hoseini","doi":"10.18502/ijrm.v22i10.17670","DOIUrl":"10.18502/ijrm.v22i10.17670","url":null,"abstract":"<p><strong>Background: </strong>Testicular ischemia/reperfusion injury, a significant result of testicular torsion, can lead to the risk of male infertility.</p><p><strong>Objective: </strong>The current study aimed to evaluate the effect of human amniotic membrane-derived mesenchymal stem cells (hAMSCs) secretome on testicular torsion/detorsion (T/D) in mice.</p><p><strong>Materials and methods: </strong>All the experiments were performed in the Anatomy Department of Tehran University of Medical Sciences, Tehran, Iran, during the period of March 2023 to December 2023. 40 male NMRI mice (5-7 wk, 25-30 gr) were randomized into: 1) the sham group: mice received sham operations with no other interventions, 2) T/D group, 3) negative control group; torsion detorsion + intratesticular injection of Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12, and 4) the T/D group + hAMSCs secreted factors. Serum testosterone levels, hematoxylin and eosin staining, and sperm quality parameters were used to evaluate the therapeutic effects of hAMSCs secreted factors on the testicular structure and function. Tissue oxidative stress was measured by determining malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase-1. Nuclear factor erythroid 2-related factor 2, Kelch-like ECH-associated protein 1, NAD-dependent deacetylase sirtuin-1, tumor necrosis factor-alpha and tumor protein P53 mRNA expressions were assessed in testis via real-time polymerase chain reaction.</p><p><strong>Results: </strong>The results showed that hAMSCs secreted factors alleviated testicular T/D injury by attenuating oxidative stress, inflammatory response, and apoptosis via modulating the sirtuin-1/ nuclear factor erythroid 2-related factor 2/tumor necrosis factor-alpha signaling pathway.</p><p><strong>Conclusion: </strong>hAMSCs secreted factors increased antioxidative, anti-inflammatory, and antiapoptotic properties which consequently increased testosterone levels, spermatogenesis, and sperm quality parameters.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 10","pages":"821-836"},"PeriodicalIF":1.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal and neonatal outcomes in women with COVID-19: A retrospective cohort study.","authors":"Malihe Nourollahpour Shiadeh, Ommolbanin Zare, Mahmood Moosazadeh, Azadeh Kiapour, Sima Elyasi","doi":"10.18502/ijrm.v22i10.17667","DOIUrl":"10.18502/ijrm.v22i10.17667","url":null,"abstract":"<p><strong>Background: </strong>Considering the conflicting evidence that exists regarding the effect of the coronavirus disease 2019 (COVID-19) on pregnancy and newborn outcomes, it is necessary to conduct this research during the first year in the north of Iran, a highly visited and trafficked place, which increases the possibility of contracting the virus and severity of the infection.</p><p><strong>Objective: </strong>This study aimed to compare maternal and neonatal outcomes in pregnant women with and without COVID-19.</p><p><strong>Materials and methods: </strong>In this retrospective cohort study, data from 160 pregnant women referred to 3 hospitals in Mazandaran, Iran from April 2020 to March 2021 were extracted from their medical records using checklists. Participants were divided into 2 groups: group A) 80 pregnant women with COVID-19 and group B) 80 noninfected pregnant women. All information related to maternal and neonatal outcomes were extracted and compared from the files of the 2 groups. The results obtained from comparing 2 groups of pregnant women from the result of the reverse-transcription polymerase chain reaction test were analyzed.</p><p><strong>Results: </strong>The most common symptoms in the COVID-19 group were fever and cough. Pregnancy complications such as abortion, pre-eclampsia, and premature birth were not significant between groups (p = 0.4, 0.45, 0.45, respectively). No statistically significant difference was observed between groups regarding maternal and neonatal outcomes.</p><p><strong>Conclusion: </strong>Despite the peak and severity of the COVID-19 disease in the first year of the pandemic, it seems that it had no effect on maternal and neonatal outcomes in each trimester of pregnancy in affected women.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 10","pages":"793-800"},"PeriodicalIF":1.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing intra-uterine injection of mononuclear cells and platelet-rich plasma on the pregnancy rate of women with recurrent implantation failure: An RCT.","authors":"Hoda Fazaeli, Azar Sheikholeslami, Zahra Ebrahimi, Naser Kalhor, Leila Naserpour","doi":"10.18502/ijrm.v22i10.17668","DOIUrl":"10.18502/ijrm.v22i10.17668","url":null,"abstract":"<p><strong>Background: </strong>Recurrent implantation failure (RIF) can be explained mainly by improper crosstalk between the embryo and endometrium. The T-helper1/T-helper2 profile balance influences effective embryo implantation. Endometrial immunomodulation via intrauterine injection of activated peripheral blood mononuclear cells (PBMCs) or autologous platelet-rich plasma (PRP) is a potentially efficient treatment option.</p><p><strong>Objective: </strong>This study aims to examine the biochemical and clinical pregnancies resulting from the intrauterine administering of activated PBMCs and PRP in RIF women.</p><p><strong>Materials and methods: </strong>This randomized clinical trial study was done in the Rooya Infertility Treatment Center, Qom, Iran from November 2022 to April 2024. 96 women with at least 2 RIFs were randomized into control, PBMC, and PRP groups. Briefly, 3 ml of blood sample was collected and PBMCs were isolated using Ficoll separation solution, and cultured for 72 hr. PRP was separated from 10 ml of peripheral blood through centrifugation. 2 days before embryo transfer PBMCs or PRP were transferred into the endometrial cavity.</p><p><strong>Results: </strong>Except for the duration of infertility, which was higher in the PBMC group, all other baseline characteristics were not statistically different. Moreover, a significantly higher rate of biochemical pregnancy was observed in the PRP (10/32) and PBMC (12/32) groups compared to the control (3/32) (p = 0.027), while the rate of clinical pregnancy was only significantly higher in the PBMC group (10/32) than in the control group (2/32) (p = 0.038).</p><p><strong>Conclusion: </strong>Neither PBMC nor PRP interventions exhibited a substantial advantage over one another regarding biochemical and clinical pregnancy rates.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 10","pages":"801-810"},"PeriodicalIF":1.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}