International Journal of Reproductive Biomedicine最新文献

{"title":"Effect of sequential cleavage and blastocyst embryo transfer compared to single cleavage stage embryo transfer on assisted reproductive technology outcome: An RCT.","authors":"Nahid Homayoon, Sahereh Arabian, Esmat Mangoli, Fatemeh Bayati, Maryam Eftekhar","doi":"10.18502/ijrm.v22i6.16793","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16793","url":null,"abstract":"<p><strong>Background: </strong>Assisted reproductive technology (ART), offers hope for many infertile couples by increasing the chance of successful pregnancy. The success of in vitro fertilization depends on various factors, in which embryo transfer (ET) is one of the critical steps influencing in vitro fertilization success rates. Extended embryo culture and blastocyst-stage ET have been considered in ART due to their potential benefits including improved implantation rates.</p><p><strong>Objective: </strong>This study aimed to compare the outcomes of sequential ET vs single cleavage-stage ET in women undergoing a fresh ET cycle with a limited number of embryos.</p><p><strong>Materials and methods: </strong>This randomized clinical trial was conducted on 140 women undergoing infertility treatments and candidates for fresh ET at the Research and Clinical Center for Infertility, Yazd, Iran from August 2023 to January 2024. Women with a number of embryos from 2-5 ( <math><mo>≥</mo></math> 2 and <math><mo>≤</mo></math> 5 available embryos) were randomly divided into 2 groups: One group underwent sequential ET (one cleavage-stage ET followed by one blastocyst ET) and the other group underwent single-step 2 cleavage-stage ET. The primary outcome was clinical pregnancy, and the secondary outcome included chemical pregnancy and early abortion rates.</p><p><strong>Results: </strong>Our findings showed significantly higher rates of clinical (33.5% vs 13.6%, p = 0.003) and chemical (41.3% vs 18.2%, p = 0.004) pregnancies in the sequential ET group compared to the single-step cleavage ET group. The early abortion rate was higher in single-step cleavage ET (13% vs 44.4%, p = 0.053).</p><p><strong>Conclusion: </strong>Sequential fresh ET is a useful choice in women who have a limited number of embryos and can improve ART outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"433-440"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-free in vitro activation and autologous transplantation in infertile women with diminished ovarian reserve: An experimental pilot study.","authors":"Saghar Salehpour, Mahsa Kazemi, Sedigheh Hosseini, Nazanin Hajizadeh, Bahareh Karimi, Hajar Abbasi, Mitra Nemati, Samaneh Esmaeili, Hasti Ziaee","doi":"10.18502/ijrm.v22i6.16792","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16792","url":null,"abstract":"<p><strong>Background: </strong>Poor ovarian response and diminished ovarian reserves (DOR) significantly contribute to female infertility. Previous attempts have been made to enhance follicular growth and improve pregnancy outcomes in these participants.</p><p><strong>Objective: </strong>This study aimed to assess the efficacy of the in vitro drug-free activation technique of the ovarian reservation and in vitro fertilization stimulation cycle outcomes in DOR participants.</p><p><strong>Materials and methods: </strong>This pilot phase study investigated the impact of in vitro activation (IVA) on ovarian reservation and in vitro fertilization outcome in 10 infertile women with DOR from May to December 2023 at Taleghani Infertility Center, Tehran, Iran. Participants underwent general surgery and laparoscopy, involving the removal of a portion of one ovary, immediate transfer to the laboratory, dissection into small cubes, and subsequent re-implantation into the cases's ovary. The primary outcomes, include the count of retrieved oocytes, the number of oocytes reaching metaphase, and the secondary outcomes were the quantity and the number of embryos transferred, implantation rate, and occurrence of clinical pregnancy.</p><p><strong>Results: </strong>The study revealed a significant increase in the antral follicle count before and after IVA (p = 0.033). Before IVA, the median estradiol level was 93.5 (57.0), which reduced to 79.0 (35.0) after IVA, indicating a statistically significant difference. On average, 2.3 (0.8) oocytes were retrieved, among which 1.5 (0.7) were metaphase II oocytes. The observed pregnancy rate among the 2 cases was 22.2%.</p><p><strong>Conclusion: </strong>The current study suggests that IVA may positively impact follicular growth and pregnancy outcomes among women with DOR.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"425-432"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulatory roles of Smad2/3 protein and <i>SMURF2</i> gene expression in granulosa cells of germinal vesicle and metaphase II oocytes in polycystic ovarian syndrome: A case-control study.","authors":"Marzieh Ghorbani, Marzieh Sanoee Farimani, Iraj Khodadadi, Sina Mohagheghi, Iraj Amiri, Heidar Tayebinia","doi":"10.18502/ijrm.v22i6.16794","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16794","url":null,"abstract":"<p><strong>Background: </strong>The impaired functions of granulosa cells (GCs) in the delayed development and immaturity of oocytes have been reported in polycystic ovary syndrome (PCOs). Even with ovarian stimulation, a large number of oocytes in these patients are still in the stage germinal vesicle (GV).</p><p><strong>Objective: </strong>The levels of Smad2/3, phosphorylated Smad2/3 (P-Smad2/3), the expression of <i>SARA</i>, <i>Smad4</i>, and <i>SMURF2</i> genes in the GCs surrounding metaphase II (MII) or GV oocytes in PCOs women were investigated.</p><p><strong>Materials and methods: </strong>GCs of MII and GV oocytes were isolated from 38 women with PCOs and the expression levels of <i>SARA, Smad4,</i> and <i>SMURF2</i> in surrounding GCs of MII and GV oocytes were determined using reverse-transcription polymerase chain reaction<i>.</i> Also, Smad2/3 and P-Smad2/3 proteins were determined using western blotting.</p><p><strong>Results: </strong>The expression level of <i>SMURF2</i> was significantly higher in GCs surrounding GV oocytes compared with that of GCs encompassing MII oocytes (p <math><mo><</mo></math> 0.001). At the same time, no significant differences were observed in <i>SARA</i> and <i>Smad4</i> expression levels in GCs surrounding GV and MII oocytes. A lower level of P-Smad2/3 was also found in GCs GV oocytes compared with GCs of MII oocytes (p <math><mo><</mo></math> 0.001).</p><p><strong>Conclusion: </strong>It seems that P-Smad2/3 plays a role in oocyte development, and the downregulation of this protein is associated with a defect in the maturation of GV oocytes. On the other hand, the upregulation of the <i>SMURF2</i> gene also affects the growth process of GCs and the maturation of GV oocytes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"441-450"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometriosis and adverse pregnancy outcomes: A case-control study.","authors":"Fatemeh Shahmoradi, Ladan Haghighi, Marziyeh Noori, Roya Derakhshan, Neda Hashemi, Samaneh Rokhgireh","doi":"10.18502/ijrm.v22i6.16798","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16798","url":null,"abstract":"<p><strong>Background: </strong>The association between endometriosis and the outcome of pregnancy is one of the interesting topics. Endometriosis-related pain is alleviated with pregnancy; however, it is known to cause adverse outcomes in pregnancy. The main cause is systemic chronic inflammation caused by higher levels of cytokines, growth factors, and angiogenesis factors.</p><p><strong>Objective: </strong>This study aimed to clarify the relationship between endometriosis, deep endometriosis, adenomyosis, surgical treatment, and poor maternal consequences.</p><p><strong>Materials and methods: </strong>In this case-control study, data from 250 women who gave birth in Hazrat Rasoul Akram hospital, Tehran, Iran from February 2015 to December 2019 was extracted from the hospital information system in January 2020. Participants were divided into 2 groups: 125 women with endometriosis and 125 women without endometriosis. We looked at how endometriosis affected mothers and newborn babies. Data on pregnancy, delivery, and newborns of both groups was extracted.</p><p><strong>Results: </strong>The mean age of participants was 32.74 <math><mo>±</mo></math> 4.10 and 31.7 <math><mo>±</mo></math> 5.53 yr in endometriosis and control group, respectively. In terms of pregnancy complications, placenta previa, placenta accreta, placenta abruption, pre-eclampsia, gestational diabetes mellitus, and postpartum hemorrhage remarkably increased in the endometriosis group compared to the control group. Small for gestational age was significantly higher in rectal endometriosis than women without rectal endometriosis (p = 0.03). The neonatal intensive care unit admission rate was notably higher in infants of the endometriosis group compared to controls (40.7% vs. 24.8%, p = 0.009).</p><p><strong>Conclusion: </strong>Our findings showed women with endometriosis are at a higher risk for important adverse maternal outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"473-480"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the gene expression profiles of endometrial and trophoblastic cells in women with recurrent miscarriage: A bioinformatics approach.","authors":"Kambiz Ahmadi, Somayeh Reiisi, Zahra Habibi","doi":"10.18502/ijrm.v22i6.16800","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16800","url":null,"abstract":"<p><strong>Background: </strong>Recurrent miscarriage (RM) remains unsolved in <math><mo>></mo></math> 50% of patients and causes physical and psychological problems in women without specific risk factors for miscarriage. For a successful pregnancy, acceptance of the endometrium and invasion of trophoblast cells into the endometrium is necessary.</p><p><strong>Objective: </strong>This study aimed to use computational analysis to identify key genes and related pathways in endometrial and trophoblast cells derived from RM samples.</p><p><strong>Materials and methods: </strong>In this bioinformatics study, we explored the differential expression of genes in endometrial and trophoblast cells by analyzing the GSE165004 and GSE76862 datasets, respectively with the limma package in R software. Subsequently, overlapped genes between 2 datasets were selected, gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed. The overlapped genes were integrated to construct a protein-protein interaction network and hub genes selection.</p><p><strong>Results: </strong>We observed 41 overlapped genes between endometrial and trophoblast cells, and future analysis was accomplished in overlapped and nonoverlapped genes. Kyoto Encyclopedia of Genes and Genomes analysis indicated that overlapped genes were significantly enriched in the complement and coagulation cascades, pluripotency of stem cells, and synthesis and degradation of ketone bodies. Gene ontology analysis suggested that the genes were enriched in the cell cycle, apoptosis, and cell division. The top 10 genes included: <i>IRS1, FGF2, MAPK6, MAPK1, MAPK3, MAPK8, MAPK9, PLK1, PRKACA</i>, and <i>PRKCA</i> were identified from the PPI network.</p><p><strong>Conclusion: </strong>This study identified the key genes and potential molecular pathways underlying the development of RM. This could provide novel insights to determine the possible mechanisms and interventional strategies associated with miscarriage.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"495-506"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental accelerating testicular tissue recovery post-methotrexate treatment in rats: A promising role of Sertoli cell-conditioned medium: An experimental study.","authors":"Aref Delkhosh, Fatemeh Shabani, Masoud Delashoub","doi":"10.18502/ijrm.v22i4.16390","DOIUrl":"10.18502/ijrm.v22i4.16390","url":null,"abstract":"<p><strong>Background: </strong>Methotrexate (MET) is one of the most important chemotherapy agents used against various tumors and cancer diseases. One of the critical side effects of MET is inducing male infertility.</p><p><strong>Objective: </strong>The current study aimed to investigate Sertoli cell culture-conditioned medium (SCM) recovery effects on MET-induced conditions in rats.</p><p><strong>Materials and methods: </strong>30 mature male Wistar rats were randomly divided into 3 groups (n = 10). In the first group, rats received normal saline intraperitoneally. In the second group, animals received MET (10 mg/kg; intraperitoneally) once a week for 2 wk. The rats in the third group (MET+SCM) received MET and a single injection of SCM for 56 days post-MET administration. 56 days later, serum, epididymis, and testicular tissue samples were collected, and the animals were euthanized. Sperm parameters, serum levels of luteinizing hormone, follicle-stimulating hormone, and testosterone were examined. The testicular tissues were stained using hematoxylin and eosin solution, and histopathological changes were analyzed.</p><p><strong>Results: </strong>The MET-induced condition resulted in significant pathological changes in the testis, decreased hormone levels, and downregulated sperm parameters. However, SCM injection improved hormonal levels, testicular changes, and sperm parameters.</p><p><strong>Conclusion: </strong>It can be concluded that a single intra-testicular SCM injection accelerates male reproductive system recovery post-MET treatment.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 4","pages":"295-304"},"PeriodicalIF":1.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11255466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study.","authors":"Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen","doi":"10.18502/ijrm.v22i4.16391","DOIUrl":"10.18502/ijrm.v22i4.16391","url":null,"abstract":"<p><strong>Background: </strong>Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in vitrification on sperm motility and DNA integrity are still unclear.</p><p><strong>Objective: </strong>This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries.</p><p><strong>Materials and methods: </strong>Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups: vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested.</p><p><strong>Results: </strong>Sperm motility in vitrified media with CPAs (54.4 <math><mo>±</mo></math> 11%) was statistically higher than in media without CPAs (51.14 <math><mo>±</mo></math> 10.6%, p <math><mo><</mo></math> 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 <math><mo>±</mo></math> 8.47 vs. 70.38 <math><mo>±</mo></math> 8.11 and 50.84 <math><mo>±</mo></math> 18.92 vs. 51.98 <math><mo>±</mo></math> 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 <math><mo>±</mo></math> 8.47 vs. 12.60 <math><mo>±</mo></math> 9.03, p = 0.021).</p><p><strong>Conclusion: </strong>Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 4","pages":"305-316"},"PeriodicalIF":1.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11255465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulating the RFamide-related peptide-3/G protein-coupled receptor 147 signaling pathway with nourishing Yin-removing fire herbal mixture to alleviate precocious puberty in female rats: An experimental study.","authors":"Xiaoli Dai, Yuanyuan He, Suhuan Li, Yanyan Sun, Wen Sun, Zhanzhuang Tian, Ph D, Jian Yu, Ph D, Nurgul Ablakimova, Yonghong Wang","doi":"10.18502/ijrm.v22i1.15240","DOIUrl":"10.18502/ijrm.v22i1.15240","url":null,"abstract":"<p><strong>Background: </strong>Precocious puberty (PP) involves early activation of the hypothalamic gonadotropin-releasing hormone (GnRH) generator. The RFamide-related peptide/G protein-coupled receptor 147 (<i>RFRP3/GPR147</i>) signaling pathway is vital in inhibiting GnRH and delaying puberty onset. The nourishing Yin-removing fire (NYRF) herbal mixture has shown promising results in treating PP.</p><p><strong>Objective: </strong>This study aimed to assess the impact of the NYRF herbal mixture on the <i>RFRP3/GPR147</i> signaling pathway in the hypothalamus and its potential in alleviating PP in female rats.</p><p><strong>Materials and methods: </strong>In a controlled experiment, 24 female Sprague-Dawley rats (11.20 <math><mo>±</mo></math> 0.69 gr, postnatal day [PD5]) were divided into normal, model, normal saline, and NYRF groups (n = 6/each). PP was induced in the model, normal saline, and NYRF groups by subcutaneous injection of danazol at PD5. The NYRF herbal mixture or normal saline was administered from PD15. Serum sex hormone levels and hypothalamic samples were collected for mRNA and protein expression at PD30.</p><p><strong>Results: </strong>In the model group, hypothalamic GnRH and kisspeptin levels increased, while RFRP3 and GPR147 levels decreased, luteinizing hormone levels elevated, reproductive organ coefficients increased, and the vagina opened earlier compared to the normal group. Conversely, the NYRF group exhibited lower GnRH and kisspeptin levels but higher RFRP3 levels in the hypothalamus. Serum luteinizing hormone levels were reduced, reproductive organ coefficients were reduced, and the vaginal opening was delayed compared to the model and normal saline groups.</p><p><strong>Conclusion: </strong>The NYRF herbal mixture delayed sexual development in rats with PP by hypothalamic upregulating RFRP3 and downregulating GnRH and kisspeptin.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 1","pages":"31-42"},"PeriodicalIF":1.3,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic demyelination interferes with normal spermatogenesis in cuprizone-intoxicant C57/BL 6 mice: An experimental study.","authors":"Arezoo Dorikhani, Ameneh Omidi, Mansoureh Movahedin, Iman Halvaei","doi":"10.18502/ijrm.v22i1.15241","DOIUrl":"10.18502/ijrm.v22i1.15241","url":null,"abstract":"<p><strong>Background: </strong>Due to myelin and axonal insults in multiple sclerosis individuals, motor coordination problems and endocrine imbalance may develop.</p><p><strong>Objective: </strong>This study aims to evaluate the role of chronic demyelination on the hypothalamic-pituitary-gonadal axis in the mouse model of multiple sclerosis.</p><p><strong>Materials and methods: </strong>20 adult C57/BL6 male mice were divided into 2 groups (n = 10/each) as follows: the control group (CONT) received a regular diet for 17 wk; and the experimental group (cuprizone [CPZ]) was fed with 0.2% CPZ for 12 wk and, then CPZ was withdrawn for 5 wk. Serum testosterone, histopathology of the brain and testis, and sperm analysis were evaluated.</p><p><strong>Results: </strong>The hypothalamic myelin content was significantly decreased in the arcuate nucleus following the 12 wk of CPZ consumption compared to the CONT group, and the statistical difference remained until 17 wk. Testosterone levels declined significantly in the CPZ group compared to the CONT group in the 12<math><msup><mrow><mi> </mi></mrow><mtext>th</mtext></msup></math> and 17<math><msup><mrow><mi> </mi></mrow><mtext>th</mtext></msup></math> wk. A significant decrease was observed in the height of the seminiferous epithelium and the interstitial tissue area, and the number of seminiferous epithelial cells in the CPZ group compared to the CONT group in the 12<math><msup><mrow><mi> </mi></mrow><mtext>th</mtext></msup></math> and 17<math><msup><mrow><mi> </mi></mrow><mtext>th</mtext></msup></math> wk. The sperm count, motility, and viability in the CPZ group significantly decreased compared to the CONT group in the 12<math><msup><mrow><mi> </mi></mrow><mtext>th</mtext></msup></math> and 17<math><msup><mrow><mi> </mi></mrow><mtext>th</mtext></msup></math> wk of the study.</p><p><strong>Conclusion: </strong>Chronic demyelination induced by CPZ intoxication, maybe through damage to the hypothalamus arcuate nucleus, leads to the hypothalamic-pituitary-gonadal axis disturbance and damage to the testis and spermatogenesis subsequently.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 1","pages":"43-54"},"PeriodicalIF":1.3,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of diagnostic value of pelvic MRI in endometriosis in comparison with surgical findings: A cross-sectional study.","authors":"Reza Nafisi Moghadam, Fatemeh Tamizi, Seid Kazem Razavi Ratki, Amin Nafisi Moghadam, Atiyeh Javaheri, Nasim Namiranian","doi":"10.18502/ijrm.v22i1.15242","DOIUrl":"10.18502/ijrm.v22i1.15242","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is a multifocal gynecologic disorder during the fertility period in women. Magnetic resonance imaging (MRI) is an important diagnostic modality for this disease and can either be used alone or along with transvaginal ultrasonography.</p><p><strong>Objective: </strong>This study aims to compare the accuracy of pelvis MRI in pelvic deep endometriosis with laparoscopic findings in women referred to Shahid Sadoughi hospital in one year.</p><p><strong>Materials and methods: </strong>This cross-sectional study was conducted on 40 women suspicious of endometriosis who referred to Shahid Sadoughi hospital, Yazd, Iran from November 2020-2021. Based on clinical findings and history, participants were referred to the imaging center for pelvic MRI. Finally, the results of MRI and diagnostic laparoscopy were compared with pathologic findings.</p><p><strong>Results: </strong>The sensitivity and specificity of MRI for pelvic endometriosis were 94.8% and 20%, respectively. Also, the positive predictive value and negative predictive value of MRI were 90.2% and 33.3%, respectively.</p><p><strong>Conclusion: </strong>Laparoscopy is still the gold standard of endometriosis diagnosis, but MRI with susceptibility-weighted imaging sequence is the best noninvasive diagnostic method.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 1","pages":"55-60"},"PeriodicalIF":1.3,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}