Chrysin's protective effect on the expression of protamine, Tsga10, Dazl, and Akap4 genes against diazinon toxin in male rats: An experimental study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY

International Journal of Reproductive Biomedicine Pub Date : 2025-06-10 eCollection Date: 2024-04-01 DOI:10.18502/ijrm.v23i3.18778

Fatemeh Mojibi, Esmail Fattahi, Seyed Gholam Ali Jorsaraei, Sohrab Kazemi, Maryam Gholamitabar Tabari

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引用次数: 0

Abstract

Background: Environmental pollution due to pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D).

Objective: To investigate the protective effects of C on the expression of protamine, testis-specific gene antigen 10 (Tsga10), deleted azoospermia-like gene (Dazl), and A-Kinase anchoring protein 4 (Akap4) genes in the testicular tissue of rats receiving D.

Materials and methods: In this experimental study, 42 Wistar rats (7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, Amol Research Center, Amol, Iran. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1) control: without any injection. 2) Sham: injection of 10% tween 80 solution as D solvent. 3) D: injection at a concentration of 20 mg/kg/BW. 4 and 5) C with low dose (C10 mg/kg/BW) and high dose (C20 mg/kg/BW). 6 and 7) D with low dose of C (D20 mg/kg/BW/ C10 mg/kg/BW) and D with high dose: (D20 mg/kg/BW/ C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.

Results: Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of Dazl (p $<$ 0.0001), Tsga10 (p = 0.01), and protamine (p $<$ 0.0001) genes, but increased the expression of Akap4 gene (p $<$ 0.0001).

Conclusion: D has a negative effect on the expression of meiotic genes in rat testicular tissue, while C greatly reduces the adverse effects caused by D.

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菊花素对雄性大鼠抗重嗪农毒素蛋白蛋白、Tsga10、Dazl和Akap4基因表达的保护作用的实验研究

背景：农药引起的环境污染是世界性的主要健康问题。菊花素(C)是一种黄酮类化合物，可以减少由二嗪醌(D)引起的不良反应。目的：探讨C对d治疗大鼠睾丸组织中鱼精蛋白、睾丸特异性基因抗原10 （Tsga10）、缺失无精子样基因（Dazl）和a激酶锚定蛋白4 （Akap4）基因表达的保护作用。材料和方法：本实验选用42只Wistar大鼠（7-8周，180-250 gr）。该研究于2021年在伊朗阿莫尔研究中心伊朗巴斯德研究所进行。每周注射5天，连续4周。大鼠随机分组：1)对照组：不注射。2)假手术：注射10%的吐温80溶液作为D溶剂。3) D：注射浓度为20mg /kg/BW。4和5)C低剂量（C10 mg/kg/BW）和高剂量（C20 mg/kg/BW）。D低剂量组（D20 mg/kg/BW/ C10 mg/kg/BW）， D高剂量组（D20 mg/kg/BW/ C20 mg/kg/BW）。最后，对睾丸进行组织学和基因表达检查。结果：组织学结果显示，D通过减少生发细胞、直径和管腔面积，改善了C干预后的精子发生。SPSS结果显示，D降低了Dazl （p 0.0001）、Tsga10 （p = 0.01）和鱼精蛋白（p 0.0001）基因的表达，但增加了Akap4基因的表达（p 0.0001）。结论：D对大鼠睾丸组织减数分裂基因的表达有负面影响，而C则大大减轻了D的不良影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Reproductive Biomedicine OBSTETRICS & GYNECOLOGY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

16 weeks

期刊介绍： The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.