Variation of the encoding hyaluronic receptors Hyaluronan-mediated motility receptor (rs299295) and Stabilin-2 (rs2271637) genes with prostate neoplasms risk: A case-control and in silico study.
{"title":"Variation of the encoding hyaluronic receptors Hyaluronan-mediated motility receptor (rs299295) and Stabilin-2 (rs2271637) genes with prostate neoplasms risk: A case-control and in silico study.","authors":"Hayder Abdulhadi Saleh Albdairi, Abasalt Hosseinzadeh Colagar","doi":"10.18502/ijrm.v23i3.18776","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hyaluronan-mediated motility receptor (HMMR) and Stabilin-2 (STAB2), known as extracellular matrix cell surface protein's receptors, bind to hyaluronic acid and lead to various cell functions.</p><p><strong>Objective: </strong>The study aims to investigate the relationship between the <i>HMMR</i>-rs299295 (C <math><mo>></mo></math> T/ A485V) and <i>STAB2</i>-rs2271637 (C <math><mo>></mo></math> G/ L2401V) gene variants and the risk of prostate neoplasms in the Mazandaran population, North of Iran.</p><p><strong>Materials and methods: </strong>This study was conducted based on a case-control and <i>in silico</i> approach. Genomic DNA was extracted from 598 intravenous blood samples, collected from 250 benign prostatic hyperplasia (case group I) and 250 malignant prostate (case group II) neoplasms as cases, and 98 healthy men as control. The <i>HMMR</i>-rs299295 and <i>STAB2</i>-rs2271637 genotypes were identified using the polymerase chain reaction-restriction fragment length polymorphism method. Bioinformatics analyses were conducted using PolyPhen-2, GOR IV, and GeneMANIA free web tools.</p><p><strong>Results: </strong>The study found that the mutant T allele in <i>HMMR</i>-rs299295 and the G allele in <i>STAB2</i>-rs2271637 are associated with an increased risk of prostate neoplasm, including benign prostatic hyperplasia and prostate cancer (p <math><mo><</mo></math> 0.001). Bioinformatic analyses revealed structural changes and potential damage from these variants. The <i>HMMR</i>-A485V variant might impair interaction with family with sequence similarity 83 member D, and the <i>STAB2</i>-L2401V variant could disrupt domain 7 of FAS1, together they may affect the protein's physical interactions, especially with mitogen-activated protein kinase 1.</p><p><strong>Conclusion: </strong>The mutant alleles of T in <i>HMMR</i>-rs299295 and the G in <i>STAB2</i>-rs2271637 may disrupt protein structures and probably contribute to prostate neoplasm progression.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"23 3","pages":"251-264"},"PeriodicalIF":1.6000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186173/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Reproductive Biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijrm.v23i3.18776","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Hyaluronan-mediated motility receptor (HMMR) and Stabilin-2 (STAB2), known as extracellular matrix cell surface protein's receptors, bind to hyaluronic acid and lead to various cell functions.
Objective: The study aims to investigate the relationship between the HMMR-rs299295 (C T/ A485V) and STAB2-rs2271637 (C G/ L2401V) gene variants and the risk of prostate neoplasms in the Mazandaran population, North of Iran.
Materials and methods: This study was conducted based on a case-control and in silico approach. Genomic DNA was extracted from 598 intravenous blood samples, collected from 250 benign prostatic hyperplasia (case group I) and 250 malignant prostate (case group II) neoplasms as cases, and 98 healthy men as control. The HMMR-rs299295 and STAB2-rs2271637 genotypes were identified using the polymerase chain reaction-restriction fragment length polymorphism method. Bioinformatics analyses were conducted using PolyPhen-2, GOR IV, and GeneMANIA free web tools.
Results: The study found that the mutant T allele in HMMR-rs299295 and the G allele in STAB2-rs2271637 are associated with an increased risk of prostate neoplasm, including benign prostatic hyperplasia and prostate cancer (p 0.001). Bioinformatic analyses revealed structural changes and potential damage from these variants. The HMMR-A485V variant might impair interaction with family with sequence similarity 83 member D, and the STAB2-L2401V variant could disrupt domain 7 of FAS1, together they may affect the protein's physical interactions, especially with mitogen-activated protein kinase 1.
Conclusion: The mutant alleles of T in HMMR-rs299295 and the G in STAB2-rs2271637 may disrupt protein structures and probably contribute to prostate neoplasm progression.
期刊介绍:
The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.