Variation of the encoding hyaluronic receptors Hyaluronan-mediated motility receptor (rs299295) and Stabilin-2 (rs2271637) genes with prostate neoplasms risk: A case-control and in silico study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
International Journal of Reproductive Biomedicine Pub Date : 2025-06-10 eCollection Date: 2024-04-01 DOI:10.18502/ijrm.v23i3.18776
Hayder Abdulhadi Saleh Albdairi, Abasalt Hosseinzadeh Colagar
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引用次数: 0

Abstract

Background: Hyaluronan-mediated motility receptor (HMMR) and Stabilin-2 (STAB2), known as extracellular matrix cell surface protein's receptors, bind to hyaluronic acid and lead to various cell functions.

Objective: The study aims to investigate the relationship between the HMMR-rs299295 (C > T/ A485V) and STAB2-rs2271637 (C > G/ L2401V) gene variants and the risk of prostate neoplasms in the Mazandaran population, North of Iran.

Materials and methods: This study was conducted based on a case-control and in silico approach. Genomic DNA was extracted from 598 intravenous blood samples, collected from 250 benign prostatic hyperplasia (case group I) and 250 malignant prostate (case group II) neoplasms as cases, and 98 healthy men as control. The HMMR-rs299295 and STAB2-rs2271637 genotypes were identified using the polymerase chain reaction-restriction fragment length polymorphism method. Bioinformatics analyses were conducted using PolyPhen-2, GOR IV, and GeneMANIA free web tools.

Results: The study found that the mutant T allele in HMMR-rs299295 and the G allele in STAB2-rs2271637 are associated with an increased risk of prostate neoplasm, including benign prostatic hyperplasia and prostate cancer (p < 0.001). Bioinformatic analyses revealed structural changes and potential damage from these variants. The HMMR-A485V variant might impair interaction with family with sequence similarity 83 member D, and the STAB2-L2401V variant could disrupt domain 7 of FAS1, together they may affect the protein's physical interactions, especially with mitogen-activated protein kinase 1.

Conclusion: The mutant alleles of T in HMMR-rs299295 and the G in STAB2-rs2271637 may disrupt protein structures and probably contribute to prostate neoplasm progression.

透明质酸介导的运动受体(rs299295)和稳定蛋白-2 (rs2271637)基因编码与前列腺肿瘤风险的变异:一项病例对照和计算机研究
背景:透明质酸介导的运动受体(HMMR)和稳定素-2 (STAB2)被称为细胞外基质细胞表面蛋白受体,与透明质酸结合并导致各种细胞功能。目的:研究伊朗北部Mazandaran人群HMMR-rs299295 (C > T/ A485V)和STAB2-rs2271637 (C > G/ L2401V)基因变异与前列腺肿瘤风险的关系。材料和方法:本研究采用病例对照和计算机方法。从598份静脉血样中提取基因组DNA,其中250份为良性前列腺增生(病例组I), 250份为恶性前列腺肿瘤(病例组II), 98名健康男性作为对照。采用聚合酶链反应-限制性片段长度多态性方法鉴定了HMMR-rs299295和STAB2-rs2271637基因型。使用polyphen2、GOR IV和GeneMANIA免费网络工具进行生物信息学分析。结果:研究发现HMMR-rs299295突变T等位基因和STAB2-rs2271637突变G等位基因与前列腺肿瘤风险增加相关,包括良性前列腺增生和前列腺癌(p 0.001)。生物信息学分析揭示了这些变异的结构变化和潜在损害。HMMR-A485V变异可能破坏与家族序列相似的83成员D的相互作用,STAB2-L2401V变异可能破坏FAS1的7结构域,它们可能共同影响蛋白质的物理相互作用,特别是与丝裂原活化蛋白激酶1的相互作用。结论:hmrr -rs299295中突变的T等位基因和STAB2-rs2271637中突变的G等位基因可能会破坏蛋白质结构,并可能参与前列腺肿瘤的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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