International Journal of Reproductive Biomedicine最新文献

{"title":"Comments on \"Negative effect of varicocele on sperm mitochondrial dysfunction: A cross-sectional study\".","authors":"Elaheh Sanjari, Hadi Raeisi Shahraki","doi":"10.18502/ijrm.v22i7.16976","DOIUrl":"10.18502/ijrm.v22i7.16976","url":null,"abstract":"","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"605-608"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of chronic opioid on cognitive function and spermatogenesis in rat: An experimental study.","authors":"Hamid Norioun, Seyed Jamal Moshtaghian, Firoozeh Alavian, Maryam Khombi Shooshtari, Golnaz Alipour, Saeedeh Ghiasvand","doi":"10.18502/ijrm.v22i7.16971","DOIUrl":"10.18502/ijrm.v22i7.16971","url":null,"abstract":"<p><strong>Background: </strong>Opioid analgesics like morphine and methadone are widely used for managing severe pain; however, concerns over their potential misuse and adverse effects on the brain and reproductive system are significant.</p><p><strong>Objective: </strong>We aimed to investigate their impacts on spermatogenesis and cognitive function in male Norway rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 36 male Norway rats (250-300 gr, 6 months old) were divided into 6 groups: low-dose morphine, high-dose morphine, low-dose methadone, high-dose methadone, positive control (received normal saline at 5 mg/kg), and negative control (received no treatment). Morphine and methadone were administered intraperitoneally over 30 days at doses of 3 mg/kg and 7 mg/kg, respectively. Behavioral assessments evaluated anxiety, stress, and short- and long-term memory. Sperm parameters (viability, motility, morphology), hormonal analysis (testosterone, luteinizing hormone, follicle-stimulating hormone, estradiol), and gene expressions (<i>Tp53, CatSper1</i>) were assessed.</p><p><strong>Results: </strong>A significant reduction in rat weight was observed in the high-dose morphine group (p = 0.0045), while testicular weights remained unchanged. Sperm abnormalities were observed with high doses of methadone and morphine. High-dose methadone significantly reduced offspring count (p = 0.0004). Levels of follicle-stimulating hormone, luteinizing hormone, testosterone, and estradiol varied significantly across treatment groups. Gene expression was altered in response to treatments (p <math><mo><</mo></math> 0.05).</p><p><strong>Conclusion: </strong>Prolonged exposure to methadone and morphine resulted in memory dysfunction, chronic stress, hormonal disturbances, altered gene expression, and fertility complications. These effects were more pronounced at higher doses, highlighting the importance of careful dosage management in opioid therapy.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"579-592"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of <i>FSHR</i> and <i>LHCGR</i> gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study.","authors":"Jayesh Amin, Naga Sandhya Alle, Ami Patel, Bansi Prajapathi, Paresh Makwana, Jaya Prakash, Kota Murali Krishna","doi":"10.18502/ijrm.v22i7.16962","DOIUrl":"10.18502/ijrm.v22i7.16962","url":null,"abstract":"<p><strong>Background: </strong>Follicle-stimulating hormone receptor (<i>FSHR</i>) and luteinizing hormone/choriogonadotropin receptor (<i>LHCGR</i>) are integral to ovarian function, facilitating follicle development and maturation through their respective hormonal interactions. The influence of receptor polymorphisms on the outcomes of freeze-all cycles remains unclear.</p><p><strong>Objective: </strong>This study investigates the impact of <i>FSHR</i> <i>N680S</i> and <i>LHCGR</i> <i>N312S</i> polymorphisms on clinical outcomes in freeze-all cycles.</p><p><strong>Materials and methods: </strong>Women undergoing controlled ovarian stimulation for assisted reproductive technology participated in this study. They were administered a gonadotropin-releasing hormone antagonist protocol, with recombinant follicle-stimulating hormone (rFSH) dosages adjusted according to age, body mass index, antral follicle count, and individual hormonal responses. Additionally, human menopausal gonadotropin dosages were tailored based on the <i>LHCGR</i> <i>N312S</i> genetic variant.</p><p><strong>Results: </strong>Analysis revealed no significant differences in age, body mass index, antral follicle count, or marital status across the genotypes of <i>FSHR</i> <i>N680S</i> and <i>LHCGR</i> <i>N312S</i>. However, notable differences were observed in the rFSH dosage required daily and in total among the FSHR polymorphism genotypes. Genotypes of the <i>LHCGR</i> polymorphism correlated with fewer stimulation days. A significant interaction was observed between the 2 polymorphisms concerning total rFSH dosage.</p><p><strong>Conclusion: </strong>The presence of serine in the <i>FSHR</i> polymorphism was associated with higher rFSH dosage requirements. Both <i>FSHR</i> <i>N680S</i> and <i>LHCGR</i> <i>N312S</i> polymorphisms significantly influenced clinical pregnancy and live birth outcomes in freeze-all cycles, underscoring the potential of a pharmacogenomic approach to optimize hormone supplementation in controlled ovarian stimulation protocols during assisted reproductive technology treatments.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"539-552"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of progesterone and estrogen receptors in treatment choice after endometriosis surgery: A cross-sectional study.","authors":"Tahereh Poordast, Saeed Alborzi, Ziba Kiani, Navid Omidifar, Elham Askary, Kefayat Chamanara, Mansoureh Shokripour, Alimohammad Keshtvarz Hesam Abadi","doi":"10.18502/ijrm.v22i7.16970","DOIUrl":"10.18502/ijrm.v22i7.16970","url":null,"abstract":"<p><strong>Background: </strong>The lack of improvement in some endometriotic people's pain after surgery even while using hormone treatment may suggest an inappropriate response to routine hormonal therapies.</p><p><strong>Objective: </strong>This study aimed to determine a cut-off point for selecting the most appropriate treatment based on the hormone receptors of endometriotic lesions.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, by reviewing the medical records of participants and testing their archive samples and phone interviews (if needed), 86 symptomatic women after endometriosis surgery who were operated into governmental hospitals, Shahid Faghihi and Hazrate Zeinab Shiraz Iran were enrolled between March 2017 and March 2019. Women were divided into 2 groups: responsiveness (n = 73 for dysmenorrhea, n = 60 for dyspareunia) to medical treatment and surgery, and unresponsiveness (n = 13, n = 7). We examined the pathological slides of 86 women to determine the amount of hormone receptors and the relationship between the type of medical treatment and the level of hormone receptors on pain relief within 1 yr after surgery.</p><p><strong>Results: </strong>Based on the receiver operating characteristic curve, dysmenorrhea in the presence of tissue estrogen receptors <math><mo>></mo></math> 60% (p = 0.1065), and dyspareunia in the presence of tissue progesterone receptors <math><mo>></mo></math> 80% (p = 0.0001) responded well to medical treatment after surgery. In the presence of endometrioma-dysmenorrhea showed the best response to oral contraceptive pills (69.4%), while in deep infiltrative endometriosis-dyspareunia showed the best response to progesterone treatment (75%).</p><p><strong>Conclusion: </strong>Prescribing an appropriate hormone therapy based on a specific immunohistochemistry staining pattern can improve the life quality of postoperative endometriosis individuals.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"567-578"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin's protective effect against placental transfer of Methadone in mice: An experimental study.","authors":"Maryam Akbarzadeh, Ramin Ataee, Farkhondeh Nemati, Abbas Ali Dehpouri, Fatemeh Shaki","doi":"10.18502/ijrm.v22i7.16957","DOIUrl":"10.18502/ijrm.v22i7.16957","url":null,"abstract":"<p><strong>Background: </strong>Methadone is a substance widely used in the substitution treatment of opiate addiction in pregnancy. The placental transfer of methadone influences oxidative stress processes. Melatonin is a hormone with antioxidant activity.</p><p><strong>Objective: </strong>This study aimed to evaluate the protective effects of melatonin on oxidative stress induced by the transfer of transplacental methadone in mice.</p><p><strong>Materials and methods: </strong>In this experimental study, 36 female mice (2 months old, 20 <math><mo>±</mo></math> 2 gr) were divided into 6 groups (n = 6/each) of control, methadone (0.3 mg/kg intraperitoneal, single dose) and melatonin (2, 4, and 6 mg/kg/day gavage) were administered 30 min before methadone, and one group received melatonin alone (0.6 mg/kg with single injection). Administration for 10 consecutive days of the pregnancy period was done. After baby mice were born, all neonatal mice were killed by beheading or sacrificing after anesthesia. The liver tissues were extracted. The samples were then sent for studying oxidative stress markers such as lipid peroxidation, glutathione, and protein carbonyl contents. Also, we have used the immunohistochemistry method for apoptotic markers such as: BAX, Bcl2, and Caspase3 for assaying apoptosis.</p><p><strong>Results: </strong>This study has shown that methadone caused a significant decrease in glutathione concentration (p = 0.035). Also, we observed a significant increase in lipid peroxidation and protein carbonyl contents (p = 0.015, 0.025 respectively). However, melatonin treatment significantly inhibited oxidative stress markers (p = 0.025). Also, apoptosis assay has shown that melatonin could decrease BAX and Caspase 9 as apoptotic and increase Bcl2 as an antiapoptotic proteins (p = 0.015).</p><p><strong>Conclusion: </strong>Our findings have shown that melatonin has a protective effect against oxidative stress and apoptosis induced by the placental transfer of methadone via its antioxidant effects.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"515-526"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the natural killer cell subsets and their relationship with serum interferon gamma and vitamin D levels in women with stages III and IV endometriosis: A case-control study.","authors":"Samira Najafi Chamgordani, Nafiseh Esmaeil, Maryam Hashemi, Afshin Amari, Maryam Seyedtabib, Mehri Ghafourian","doi":"10.18502/ijrm.v22i7.16933","DOIUrl":"10.18502/ijrm.v22i7.16933","url":null,"abstract":"<p><strong>Background: </strong>Natural killer (NK) cells play a critical role in the pathogenesis of endometriosis. Moreover, a normal vitamin D level is remarkably associated with an optimal immune response. So, there may be a probable relationship between these factors and the endometriotic women.</p><p><strong>Objective: </strong>This study aimed to evaluate the percentage of NK cells and their subsets and their relationship with serum levels of vitamin D and interferon-gamma (IFN-γ) in women with endometriosis.</p><p><strong>Materials and methods: </strong>In this case-control study, 29 women with stage III-IV endometriosis and 30 healthy controls were enrolled. The study was conducted in the Immunology Department of Isfahan University of Medical Sciences, Isfahan, Iran between November 2021 and June 2022. The percentage of NK cells and their subsets, including CD56 <math> <msup><mrow><mi> </mi></mrow> <mtext>dim</mtext></msup> </math> CD16 <math> <msup><mrow><mi> </mi></mrow> <mo>+</mo></msup> </math> , CD56 <math> <msup><mrow><mi> </mi></mrow> <mtext>bright</mtext></msup> </math> CD16 <math> <msup><mrow><mi> </mi></mrow> <mo>-</mo></msup> </math> and CD56 <math> <msup><mrow><mi> </mi></mrow> <mtext>bright</mtext></msup> </math> CD16 <math> <msup><mrow><mi> </mi></mrow> <mtext>bright</mtext></msup> </math> were measured in the peripheral blood samples using flow cytometry. Serum levels of vitamin D and IFN-γ were also measured using the enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>The mean percentage of NK cells in women with endometriosis increased significantly compared to the control group (p = 0.03). The percentage of CD56 <math> <msup><mrow><mi> </mi></mrow> <mtext>dim</mtext></msup> </math> CD16 <math> <msup><mrow><mi> </mi></mrow> <mo>+</mo></msup> </math> (p = 0.007) and CD56 <math> <msup><mrow><mi> </mi></mrow> <mtext>bright</mtext></msup> </math> CD16 <math> <msup><mrow><mi> </mi></mrow> <mtext>bright</mtext></msup> </math> (p = 0.043) increased significantly in women with endometriosis in comparison with the control group, but the percentage of CD56 <math> <msup><mrow><mi> </mi></mrow> <mtext>bright</mtext></msup> </math> CD16 <math> <msup><mrow><mi> </mi></mrow> <mo>-</mo></msup> </math> subset was not significantly different. No relationship was observed between NK cells and their subsets with vitamin D and IFN-γ in the studied groups.</p><p><strong>Conclusion: </strong>The study of NK cell subsets and their related factors can be useful in assessing and treating women suffering from endometriosis. However, more comprehensive studies are required to draw definitive conclusions about these observations.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"593-604"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereological and biochemical effects of thymoquinone on ovarian tissue toxicity induced by silver nanoparticles in NMRI mice: An experimental study.","authors":"Seyed Mohammad Ali Shariatzadeh, Fatemeh Salmani, Hossein Moghanlo, Monireh Mahmoodi","doi":"10.18502/ijrm.v22i7.16964","DOIUrl":"10.18502/ijrm.v22i7.16964","url":null,"abstract":"<p><strong>Background: </strong>The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of <i>Nigella sativa</i>.</p><p><strong>Objective: </strong>We evaluated the efficacy of TQ on ovarian tissue following toxicity induced by AgNPs in female mice.</p><p><strong>Materials and methods: </strong>24 female NMRI mice (5-6 wk, an average weight of 33 gr) were randomly divided into 4 groups (n = 6/each): control, AgNPs (500 mg/kg, gavage), TQ (2.5 mg/kg, intraperitoneal injection), and TQ+AgNPs. Mice were treated every day for 35 days. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), luteinizing hormone, and follicle-stimulating hormone were measured. The optical disector and stereological techniques were utilized to estimate the follicular count, their volume at different developmental stages, and the structure of ovarian tissue.</p><p><strong>Results: </strong>In the AgNPs group, the serum concentrations of TAC (p = 0.01), luteinizing hormone (p <math><mo><</mo></math> 0.001), follicle-stimulating hormone, the volume of corpus luteum (p <math><mo><</mo></math> 0.001), and the number of follicles decreased significantly compared to the control group. Nevertheless, AgNPs significantly increased the MDA level. In the TQ+AgNPs group compared to the AgNPs group, a significant decrease in MDA level (p <math><mo><</mo></math> 0.001) and a significant improvement in TAC (p = 0.03), and hormonal levels, the number of primary, preantral, and antral follicles (p = 0.04), and the volume of corpus luteum (p = 0.01) were observed.</p><p><strong>Conclusion: </strong>TQ improved the number of follicles by reducing oxidative stress and lipid peroxidation in AgNPs-damaged ovarian tissue.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 7","pages":"553-566"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive correlation between <i>Bax</i> and <i>Bcl-2</i> gene polymorphisms with the risk of endometriosis: A case-control study.","authors":"Arefe Edalatian Kharrazi, Forough Forghani, Danial Jahantigh, Saeedeh Ghazaey Zidanloo, Mahnaz Rezaei, Mohsen Taheri","doi":"10.18502/ijrm.v22i6.16796","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16796","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is a chronic, gynecological disorder, and the disease's pathogenesis is still debatable. Genes related to apoptosis have been revealed to be deregulated in endometriosis.</p><p><strong>Objective: </strong>This study investigates the relationship between polymorphic variants of <i>Bax -248G</i> <math><mo>></mo></math> <i>A</i> and <i>Bcl-2</i> -938C <math><mo>></mo></math> A promoter regions with endometriosis risk in an Iranian population.</p><p><strong>Materials and methods: </strong>In this case-control study, the polymorphisms of <i>Bax</i> -248G <math><mo>></mo></math> A and <i>Bcl</i>-2 -938C <math><mo>></mo></math> A promoter regions were analyzed in 127 Iranian cases and 125 controls who were referred to Ali-ibn-Abi Taleb Educational hospital, Zahedan, Iran between May 2022 and February 2023. The genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method.</p><p><strong>Results: </strong>The frequencies of mutant allele A carriers and the A allele of <i>Bax</i> -248G <math><mo>></mo></math> A polymorphism showed about 2-fold significant increase of endometriosis risk (p = 0.04; p = 0.01, respectively). The frequencies of the mutant genotype AA and A allele carriers of <i>Bcl-2</i> -938C <math><mo>></mo></math> A polymorphism were approximately 4 and 2.5-fold higher in endometriosis compared to the control women, which were highly significant (p <math><mo>></mo></math> 0.001). Moreover, the allele A frequency of <i>Bcl-2</i> -938C <math><mo>></mo></math> A was associated with a 2-fold higher risk of endometriosis (p <math><mo>></mo></math> 0.001). Furthermore, the combination effects of these 2 single nucleotide polymorphisms showed that women with <i>Bax -248G</i> <math><mo>></mo></math> <i>A</i> GGand <i>Bcl-2 -938C</i> <math><mo>></mo></math> <i>A</i> AA variant alleles were associated with about 5 times higher risk of endometriosis (p <math><mo>></mo></math> 0.001). Notably, a significant difference was observed in mutant allele distribution between minimal/mild (stage I and II) and moderate/severe (stage III and IV) women with endometriosis disease.</p><p><strong>Conclusion: </strong>The results of our study provide evidence that <i>Bcl-2</i> -938C <math><mo>></mo></math> A and <i>Bax</i> -248G <math><mo>></mo></math> A single nucleotide polymorphisms might be associated with the risk of endometriosis.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"451-462"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of genetic variations and protein expression of β-Microsemino protein in intrauterine insemination outcome of unexplained infertile men: A case-control study.","authors":"Elham Bagherian, Sahar Jokari, Parnaz Borjian Boroujeni, Kaveh Haratian, Marjan Sabbaghian, Anahita Mohseni Meybodi","doi":"10.18502/ijrm.v22i6.16799","DOIUrl":"10.18502/ijrm.v22i6.16799","url":null,"abstract":"<p><strong>Background: </strong>Intrauterine insemination (IUI) is often the first-line treatment for unexplained infertility. <math><mi>β</mi></math> -Microsemino protein (MSMB) is an abundant protein in seminal plasma that has an inhibitory effect on spontaneous acrosome reaction.</p><p><strong>Objective: </strong>The present study aimed to evaluate <i>MSMB</i> gene variations and protein expression on IUI success rate in unexplained infertile men.</p><p><strong>Materials and methods: </strong>A case-control study was performed on 100 unexplained infertile Iranian men referred to the Royan Institute, Tehran, Iran for IUI (50 men with IUI positive result [IUI+], and 50 men with IUI negative result [IUI-]). Couples with female infertility factors (such as hormonal disorders, infrequent menstrual period, abnormality in uterus, fallopian tubes, or ovaries) and men with infections of the male accessory glands, hypogonadotropic hypogonadism, clinical varicocele, retractile testis, genital trauma, drug use, or concurrent hormonal treatment Y chromosome microdeletions, and abnormal karyotype were excluded from the study. The polymerase chain reaction sequencing was performed for the promoter and the coding regions of <i>MSMB</i> functional domains. To study the protein expression, the total protein of sperm was extracted, and sandwich enzyme-linked immunosorbent assay was performed.</p><p><strong>Results: </strong>4 variations were detected (rs12770171, rs10993994, rs2075894, and rs4517463). None of them showed significant differences between the IUI+ and IUI- groups. The mean value of protein expression did not show any differences between the groups.</p><p><strong>Conclusion: </strong>In conclusion, there is no association between genetic variations of promoter and coding regions of <i>MSMB</i> functional domains as well as its expression with IUI success in unexplained infertile men.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"481-494"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of sperm <i>BAX</i> and <i>BCL-2</i> gene expression with reproductive outcome in Oligoasthenoteratozoospermia cases undergoing intracytoplasmic sperm injection: A case-control study.","authors":"Fatemeh Babaei, Maryam Khoshsokhan Muzaffar, Rahil Jannatifar","doi":"10.18502/ijrm.v22i6.16797","DOIUrl":"https://doi.org/10.18502/ijrm.v22i6.16797","url":null,"abstract":"<p><strong>Background: </strong>The B-cell lymphoma 2 (<i>BCL-2</i>) protein is one of the members of the <i>BCL-2</i> associated X (<i>BAX</i>) protein family that acts as an inducer of apoptosis.</p><p><strong>Objective: </strong>The present study aims to investigate the association between <i>BAX</i> and <i>BCL-2</i> gene expression with reproductive outcome, in cases undergoing intracytoplasmic sperm injection.</p><p><strong>Materials and methods: </strong>In this case-control study, 50 men were divided into healthy fertile and oligoasthenoteratozoospermic infertile men (n = 25/each). They were subjected to history taking, clinical examination, and semen analysis. Expression of <i>BAX</i> and <i>BCL-2</i> genes were measured using real-time polymerase chain reaction. The DNA fragmentation index was measured using the sperm chromatin dispersion assay technique. Using World Health Organization criteria, sperm parameters were evaluated.</p><p><strong>Results: </strong>Evaluation of apoptosis-related genes showed that oligoasthenoteratozoospermic significantly increased mRNA expression of <i>BAX</i>, and significantly decreased mRNA expression of <i>BCL-2</i>, when compared with control. Moreover, the <i>BAX/BCL-2</i> ratio was significantly higher in oligoasthenoteratozoospermic compared to the normozoospermic group (p = 0.01). Also, this study showed that the <i>BAX</i> and <i>BCL-2</i> genes expression had a significant correlation with sperm quality, and DNA fragmentation in the oligoasthenoteratozoospermic group (p = 0.01). The oligoasthenoteratozoospermic men, had a considerably lower proportion of fertilization rate and good-quality embryos at the cleavage stage than the normozoospermic subjects (p = 0.01). A significant correlation was observed between the expression of <i>BAX</i> and <i>BCL-2</i> genes, fertilization, and embryo quality (p = 0.01).</p><p><strong>Conclusion: </strong>We concluded that the sperm <i>BAX/BCL-2</i> ratio demonstrates a significant correlation with fertilization rate and embryo quality.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 6","pages":"463-472"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}