International Journal of Oral Science最新文献

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Succinate modulates oral dysbiosis and inflammation through a succinate receptor 1 dependent mechanism in aged mice 琥珀酸通过琥珀酸受体1依赖机制调节老年小鼠口腔生态失调和炎症
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-06-10 DOI: 10.1038/s41368-025-00376-6
Fangxi Xu, Yuqi Guo, Scott C. Thomas, Anish Saxena, Samantha Hwang, Mridula Vardhan, Xin Li
{"title":"Succinate modulates oral dysbiosis and inflammation through a succinate receptor 1 dependent mechanism in aged mice","authors":"Fangxi Xu, Yuqi Guo, Scott C. Thomas, Anish Saxena, Samantha Hwang, Mridula Vardhan, Xin Li","doi":"10.1038/s41368-025-00376-6","DOIUrl":"https://doi.org/10.1038/s41368-025-00376-6","url":null,"abstract":"<p>Aging involves the accumulation of various forms of molecular and cellular damage over time. Key features of aging, such as mitochondrial dysfunction, dysbiosis, and oxidative stress, are closely linked and largely driven by inflammation. This study examines the role of succinate, a key metabolite produced and utilized by cells of both host and microbes, and its receptor, succinate receptor 1 (SUCNR1), in age-related oral dysbiosis and inflammation. We examined young and aged wild-type (WT) and SUCNR1 knockout (KO) mice for this analysis. Our findings revealed significant aging-associated alveolar bone loss and succinate elevation in aged WT mice, along with notable changes in the oral microbiome. Conversely, aged KO mice showed reduced bone loss, lower succinate levels, less inflammation, and better-maintained microbial function. These results suggest that SUCNR1 is crucial in influencing aging-related succinate elevation, oral dysbiosis, and inflammation. Analysis of gene families and pathways in the oral microbiome demonstrated distinct aging-related changes between WT and KO mice, with the functional potential being preserved in the KO-aged group. This study underscores the importance of succinate elevation and signaling through SUCNR1 in regulating inflammation, alveolar bone loss, and shifts in the oral microbiome, offering potential targets for therapeutic interventions in age-related oral health issues.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"1 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expert consensus on management of instrument separation in root canal therapy 专家对根管治疗中器械分离处理的共识
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-06-09 DOI: 10.1038/s41368-025-00372-w
Yi Fan, Yuan Gao, Xiangzhu Wang, Bing Fan, Zhi Chen, Qing Yu, Ming Xue, Xiaoyan Wang, Zhengwei Huang, Deqin Yang, Zhengmei Lin, Yihuai Pan, Jin Zhao, Jinhua Yu, Zhuo Chen, Sijing Xie, He Yuan, Kehua Que, Shuang Pan, Xiaojing Huang, Jun Luo, Xiuping Meng, Jin Zhang, Yi Du, Lei Zhang, Hong Li, Wenxia Chen, Jiayuan Wu, Xin Xu, Jing Zou, Jiyao Li, Dingming Huang, Lei Cheng, Tiemei Wang, Benxiang Hou, Xuedong Zhou
{"title":"Expert consensus on management of instrument separation in root canal therapy","authors":"Yi Fan, Yuan Gao, Xiangzhu Wang, Bing Fan, Zhi Chen, Qing Yu, Ming Xue, Xiaoyan Wang, Zhengwei Huang, Deqin Yang, Zhengmei Lin, Yihuai Pan, Jin Zhao, Jinhua Yu, Zhuo Chen, Sijing Xie, He Yuan, Kehua Que, Shuang Pan, Xiaojing Huang, Jun Luo, Xiuping Meng, Jin Zhang, Yi Du, Lei Zhang, Hong Li, Wenxia Chen, Jiayuan Wu, Xin Xu, Jing Zou, Jiyao Li, Dingming Huang, Lei Cheng, Tiemei Wang, Benxiang Hou, Xuedong Zhou","doi":"10.1038/s41368-025-00372-w","DOIUrl":"https://doi.org/10.1038/s41368-025-00372-w","url":null,"abstract":"<p>Instrument separation is a critical complication during root canal therapy, impacting treatment success and long-term tooth preservation. The etiology of instrument separation is multifactorial, involving the intricate anatomy of the root canal system, instrument-related factors, and instrumentation techniques. Instrument separation can hinder thorough cleaning, shaping, and obturation of the root canal, posing challenges to successful treatment outcomes. Although retrieval of separated instrument is often feasible, it carries risks including perforation, excessive removal of tooth structure and root fractures. Effective management of separated instruments requires a comprehensive understanding of the contributing factors, meticulous preoperative assessment, and precise evaluation of the retrieval difficulty. The application of appropriate retrieval techniques is essential to minimize complications and optimize clinical outcomes. The current manuscript provides a framework for understanding the causes, risk factors, and clinical management principles of instrument separation. By integrating effective strategies, endodontists can enhance decision-making, improve endodontic treatment success and ensure the preservation of natural dentition.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"171 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144238293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The research progress on periodontitis by the National Natural Science Foundation of China 国家自然科学基金对牙周炎的研究进展
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-06-03 DOI: 10.1038/s41368-025-00371-x
Liang Xie, Qian Chen, Hao Xu, Cui Li, Jiayu Lu, Yuangui Zhu
{"title":"The research progress on periodontitis by the National Natural Science Foundation of China","authors":"Liang Xie, Qian Chen, Hao Xu, Cui Li, Jiayu Lu, Yuangui Zhu","doi":"10.1038/s41368-025-00371-x","DOIUrl":"https://doi.org/10.1038/s41368-025-00371-x","url":null,"abstract":"<p>Periodontitis has emerged as one of the most critical oral diseases, and research on this condition holds great importance for the advancement of stomatology. As the most authoritative national scientific research funding institution in China, the National Natural Science Foundation of China (NSFC) has played a pivotal role in driving the progress of periodontal science by supporting research on periodontitis. This article provides a comprehensive review of the research and development progress related to periodontitis in China from 2014 to 2023, highlighting the significant contributions of the NSFC to this field. We have summarized the detailed funding information from the NSFC, including the number of applicant codes, funded programs and the distribution of funded scholars. These data illustrate the efforts of the NSFC in cultivating young scientists and building research groups to address key challenges in national scientific research. This study offers an overview of the current hot topics, recent breakthroughs and future research prospects related to periodontitis in China.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"169 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional and distinct roles of Piezo2-mediated mechanotransduction in dental primary afferent neurons piezo2介导的力学转导在牙原代传入神经元中的功能和独特作用
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-06-03 DOI: 10.1038/s41368-025-00374-8
Pa Reum Lee, Kihwan Lee, Ji Min Park, Shinae Kim, Seog Bae Oh
{"title":"Functional and distinct roles of Piezo2-mediated mechanotransduction in dental primary afferent neurons","authors":"Pa Reum Lee, Kihwan Lee, Ji Min Park, Shinae Kim, Seog Bae Oh","doi":"10.1038/s41368-025-00374-8","DOIUrl":"https://doi.org/10.1038/s41368-025-00374-8","url":null,"abstract":"<p>Piezo2, a mechanosensitive ion channel, serves as a crucial mechanotransducer in dental primary afferent (DPA) neurons and is potentially involved in hypersensitivity to mild mechanical irritations observed in dental patients. Given Piezo2’s widespread expression across diverse subpopulations of DPA neurons, this study aimed to characterize the mechanosensory properties of Piezo2-expressing DPA neurons with a focus on distinct features of voltage-gated sodium channels (VGSCs) and neuropeptide profiles. Using whole-cell patch-clamp recordings, we observed mechanically activated action potentials (APs) and classified AP waveforms based on the presence or absence of a hump during the repolarization phase. Single-cell reverse transcription polymerase chain reaction combined with patch-clamp recordings revealed specific associations between AP waveforms and molecular properties, including tetrodotoxin-resistant VGSCs (Na<sub>V</sub>1.8 and Na<sub>V</sub>1.9) and TRPV1 expression. Reanalysis of the transcriptomic dataset of DPA neurons identified correlations between neuropeptides—including two CGRP isoforms (α-CGRP and β-CGRP), Substance P, and Galanin—and the expression of Na<sub>V</sub>1.8 and Na<sub>V</sub>1.9, which were linked to defined AP subtypes. These molecular associations were further validated in Piezo2<sup>+</sup> DPA neurons using fluorescence in situ hybridization. Together, these findings highlight the electrophysiological and neurochemical heterogeneity of Piezo2-expressing DPA neurons and their specialized roles in distinct mechanosensory signal transmission.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"19 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physiologically relevant coculture model for oral microbial-host interactions 口腔微生物-宿主相互作用的生理相关共培养模型
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-05-27 DOI: 10.1038/s41368-025-00365-9
Zeyang Pang, Nicole M. Cady, Lujia Cen, Thomas M. Schmidt, Xuesong He, Jiahe Li
{"title":"Physiologically relevant coculture model for oral microbial-host interactions","authors":"Zeyang Pang, Nicole M. Cady, Lujia Cen, Thomas M. Schmidt, Xuesong He, Jiahe Li","doi":"10.1038/s41368-025-00365-9","DOIUrl":"https://doi.org/10.1038/s41368-025-00365-9","url":null,"abstract":"<p>Understanding microbial-host interactions in the oral cavity is essential for elucidating oral disease pathogenesis and its systemic implications. In vitro bacteria-host cell coculture models have enabled fundamental studies to characterize bacterial infection and host responses in a reductionist yet reproducible manner. However, existing in vitro coculture models fail to establish conditions that are suitable for the growth of both mammalian cells and anaerobes, thereby hindering a comprehensive understanding of their interactions. Here, we present an asymmetric gas coculture system that simulates the oral microenvironment by maintaining distinct normoxic and anaerobic conditions for gingival epithelial cells and anaerobic bacteria, respectively. Using a key oral pathobiont, <i>Fusobacterium nucleatum</i>, as the primary test bed, we demonstrate that the system preserves bacterial viability and supports the integrity of telomerase-immortalized gingival keratinocytes. Compared to conventional models, this system enhanced bacterial invasion, elevated intracellular bacterial loads, and elicited more robust host pro-inflammatory responses, including increased secretion of CXCL10, IL-6, and IL-8. In addition, the model enabled precise evaluation of antibiotic efficacy against intracellular pathogens. Finally, we validate the ability of the asymmetric system to support the proliferation of a more oxygen-sensitive oral pathobiont, <i>Porphyromonas gingivalis</i>. These results underscore the utility of this coculture platform for studying oral microbial pathogenesis and screening therapeutics, offering a physiologically relevant approach to advance oral and systemic health research.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"167 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author Correction: Organoids in the oral and maxillofacial region: present and future. 作者更正:口腔颌面区域的类器官:现在和未来。
IF 10.8 1区 医学
International Journal of Oral Science Pub Date : 2025-05-27 DOI: 10.1038/s41368-025-00377-5
Yufei Wu, Xiang Li, Hanzhe Liu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shang
{"title":"Author Correction: Organoids in the oral and maxillofacial region: present and future.","authors":"Yufei Wu, Xiang Li, Hanzhe Liu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shang","doi":"10.1038/s41368-025-00377-5","DOIUrl":"10.1038/s41368-025-00377-5","url":null,"abstract":"","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"17 1","pages":"43"},"PeriodicalIF":10.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Osteomodulin modulates the inflammatory responses via the interleukin-1 receptor 1/nuclear factor-κB signaling pathway in dental pulpitis. 骨调节素通过白细胞介素-1受体1/核因子-κB信号通路调节牙髓炎的炎症反应。
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-05-26 DOI: 10.1038/s41368-025-00369-5
Yueyi Yang,Xuchen Hu,Meiling Jing,Xiaohan Zhu,Xiaoyu Liu,Wenduo Tan,Zhanyi Chen,Chenguang Niu,Zhengwei Huang
{"title":"Osteomodulin modulates the inflammatory responses via the interleukin-1 receptor 1/nuclear factor-κB signaling pathway in dental pulpitis.","authors":"Yueyi Yang,Xuchen Hu,Meiling Jing,Xiaohan Zhu,Xiaoyu Liu,Wenduo Tan,Zhanyi Chen,Chenguang Niu,Zhengwei Huang","doi":"10.1038/s41368-025-00369-5","DOIUrl":"https://doi.org/10.1038/s41368-025-00369-5","url":null,"abstract":"Pulpitis is a common infective oral disease in clinical situations. The regulatory mechanisms of immune defense in pulpitis are still being investigated. Osteomodulin (OMD) is a small leucine-rich proteoglycan family member distributed in bones and teeth. It is a bioactive protein that promotes osteogenesis and suppresses the apoptosis of human dental pulp stem cells (hDPSCs). In this study, the role of OMD in pulpitis and the OMD-induced regulatory mechanism were investigated. The OMD expression in normal and inflamed human pulp tissues was detected via immunofluorescence staining. Intriguingly, the OMD expression decreased in the inflammatory infiltration area of pulpitis specimens. The cellular experiments demonstrated that recombined human OMD could resist the detrimental effects of lipopolysaccharide (LPS)-induced inflammation. A conditional Omd knockout mouse model with pulpal inflammation was established. LPS-induced inflammatory impairment significantly increased in conditional Omd knockout mice, whereas OMD administration exhibited a protective effect against pulpitis. Mechanistically, the transcriptome alterations of OMD overexpression showed significant enrichment in the nuclear factor-κB (NF-κB) signaling pathway. Interleukin-1 receptor 1 (IL1R1), a vital membrane receptor activating the NF-κB pathway, was significantly downregulated in OMD-overexpressing hDPSCs. Additionally, the interaction between OMD and IL1R1 was verified using co-immunoprecipitation and molecular docking. In vivo, excessive pulpal inflammation in Omd-deficient mice was rescued using an IL1R antagonist. Overall, OMD played a protective role in the inflammatory response via the IL1R1/NF-κB signaling pathway. OMD may optimize the immunomodulatory functions of hDPSCs and can be used for regenerative endodontics.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"10 1","pages":"41"},"PeriodicalIF":14.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell and spatial transcriptomics reveals an anti-tumor neutrophil subgroup in microwave thermochemotherapy-treated lip cancer 单细胞和空间转录组学揭示了微波热化疗治疗的唇癌中抗肿瘤中性粒细胞亚群
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-05-13 DOI: 10.1038/s41368-025-00366-8
Bingjun Chen, Huayang Fan, Xin Pang, Zeliang Shen, Rui Gao, Haofan Wang, Zhenwei Yu, Tianjiao Li, Mao Li, Yaling Tang, Xinhua Liang
{"title":"Single-cell and spatial transcriptomics reveals an anti-tumor neutrophil subgroup in microwave thermochemotherapy-treated lip cancer","authors":"Bingjun Chen, Huayang Fan, Xin Pang, Zeliang Shen, Rui Gao, Haofan Wang, Zhenwei Yu, Tianjiao Li, Mao Li, Yaling Tang, Xinhua Liang","doi":"10.1038/s41368-025-00366-8","DOIUrl":"https://doi.org/10.1038/s41368-025-00366-8","url":null,"abstract":"<p>Microwave thermochemotherapy (MTC) has been applied to treat lip squamous cell carcinoma (LSCC), but a deeper understanding of its therapeutic mechanisms and molecular biology is needed. To address this, we used single-cell transcriptomics (scRNA-seq) and spatial transcriptomics (ST) to highlight the pivotal role of tumor-associated neutrophils (TANs) among tumor-infiltrating immune cells and their therapeutic response to MTC. <i>MNDA</i><sup>+</sup> TANs with anti-tumor activity (N1-phenotype) are found to be abundantly infiltrated by MTC with benefit of increased blood perfusion, and these TANs are characterized by enhanced cytotoxicity, ameliorated hypoxia, and upregulated <i>IL1B</i>, activating T&amp;NK cells and fibroblasts via <i>IL1B</i>-<i>IL1R</i>. In this highly anti-tumor immunogenic and hypoxia-reversed microenvironment under MTC, fibroblasts accumulated in the tumor front (TF) can recruit N1-TANs via <i>CXCL2</i>-<i>CXCR2</i> and clear N2-TANs (pro-tumor phenotype) via <i>CXCL12</i>-<i>CXCR4</i>, which results in the aggregation of N1-TANs and extracellular matrix (ECM) deposition. In addition, we construct an N1-TANs marker, <i>MX2</i>, which positively correlates with better prognosis in LSCC patients, and employ deep learning techniques to predict expression of MX2 from hematoxylin-eosin (H&amp;E)-stained images so as to conveniently guide decision making in clinical practice. Collectively, our findings demonstrate that the N1-TANs/fibroblasts defense wall formed in response to MTC effectively combat LSCC.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"121 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels 用可逆热离子交联三维水凝胶扩增功能性人唾液腺泡细胞球体
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-05-09 DOI: 10.1038/s41368-025-00368-6
Jose G. Munguia-Lopez, Sangeeth Pillai, Yuli Zhang, Amatzia Gantz, Dimitria B. Camasao, Showan N. Nazhat, Joseph M. Kinsella, Simon D. Tran
{"title":"Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels","authors":"Jose G. Munguia-Lopez, Sangeeth Pillai, Yuli Zhang, Amatzia Gantz, Dimitria B. Camasao, Showan N. Nazhat, Joseph M. Kinsella, Simon D. Tran","doi":"10.1038/s41368-025-00368-6","DOIUrl":"https://doi.org/10.1038/s41368-025-00368-6","url":null,"abstract":"<p>Xerostomia (dry mouth) is frequently experienced by patients treated with radiotherapy for head and neck cancers or with Sjögren’s syndrome, with no permanent cure existing for this debilitating condition. To this end, in vitro platforms are needed to test therapies directed at salivary (fluid-secreting) cells. However, since these are highly differentiated secretory cells, the maintenance of their differentiated state while expanding in numbers is challenging. In this study, the efficiency of three reversible thermo-ionically crosslinked gels: (1) alginate–gelatin (AG), (2) collagen-containing AG (AGC), and (3) hyaluronic acid-containing AG (AGHA), to recapitulate a native-like environment for human salivary gland (SG) cell expansion and 3D spheroid formation was compared. Although all gels were of mechanical properties comparable to human SG tissue (~11 kPa) and promoted the formation of 3D spheroids, AGHA gels produced larger (&gt;100 cells/spheroid), viable (&gt;93%), proliferative, and well-organized 3D SG spheroids while spatially and temporally maintaining the high expression of key SG proteins (aquaporin-5, NKCC1, ZO-1, α-amylase) for 14 days in culture. Moreover, the spheroids responded to agonist-induced stimulation by increasing α-amylase secretory granules. Here, we propose alternative low-cost, reproducible, and reversible AG-based 3D hydrogels that allow the facile and rapid retrieval of intact, highly viable 3D-SG spheroids.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"72 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Profiling and functional characterization of long noncoding RNAs during human tooth development 长链非编码rna在人类牙齿发育过程中的分析和功能表征
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2025-05-09 DOI: 10.1038/s41368-025-00375-7
Xiuge Gu, Wei Wei, Chuan Wu, Jing Sun, Xiaoshan Wu, Zongshan Shen, Hanzhang Zhou, Chunmei Zhang, Jinsong Wang, Lei Hu, Suwen Chen, Yuanyuan Zhang, Songlin Wang, Ran Zhang
{"title":"Profiling and functional characterization of long noncoding RNAs during human tooth development","authors":"Xiuge Gu, Wei Wei, Chuan Wu, Jing Sun, Xiaoshan Wu, Zongshan Shen, Hanzhang Zhou, Chunmei Zhang, Jinsong Wang, Lei Hu, Suwen Chen, Yuanyuan Zhang, Songlin Wang, Ran Zhang","doi":"10.1038/s41368-025-00375-7","DOIUrl":"https://doi.org/10.1038/s41368-025-00375-7","url":null,"abstract":"<p>The regulatory processes in developmental biology research are significantly influenced by long non-coding RNAs (lncRNAs). However, the dynamics of lncRNA expression during human tooth development remain poorly understood. In this research, we examined the lncRNAs present in the dental epithelium (DE) and dental mesenchyme (DM) at the late bud, cap, and early bell stages of human fetal tooth development through bulk RNA sequencing. Developmental regulators co-expressed with neighboring lncRNAs were significantly enriched in odontogenesis. Specific lncRNAs expressed in the DE and DM, such as <i>PANCR</i>, <i>MIR205HG</i>, <i>DLX6-AS1</i>, and <i>DNM3OS</i>, were identified through a combination of bulk RNA sequencing and single-cell analysis. Further subcluster analysis revealed lncRNAs specifically expressed in important regions of the tooth germ, such as the inner enamel epithelium and coronal dental papilla (CDP). Functionally, we demonstrated that CDP-specific <i>DLX6-AS1</i> enhanced odontoblastic differentiation in human tooth germ mesenchymal cells and dental pulp stem cells. These findings suggest that lncRNAs could serve as valuable cell markers for tooth development and potential therapeutic targets for tooth regeneration.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"56 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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