International Journal of Oral Science最新文献

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Organoids in the oral and maxillofacial region: present and future. 口腔颌面部的器官组织:现状与未来。
IF 10.8 1区 医学
International Journal of Oral Science Pub Date : 2024-11-01 DOI: 10.1038/s41368-024-00324-w
Yufei Wu, Xiang Li, Hanzhe Liu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shang
{"title":"Organoids in the oral and maxillofacial region: present and future.","authors":"Yufei Wu, Xiang Li, Hanzhe Liu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shang","doi":"10.1038/s41368-024-00324-w","DOIUrl":"10.1038/s41368-024-00324-w","url":null,"abstract":"<p><p>The oral and maxillofacial region comprises a variety of organs made up of multiple soft and hard tissue, which are anatomically vulnerable to the pathogenic factors of trauma, inflammation, and cancer. The studies of this intricate entity have been long-termly challenged by a lack of versatile preclinical models. Recently, the advancements in the organoid industry have provided novel strategies to break through this dilemma. Here, we summarize the existing biological and engineering approaches that were employed to generate oral and maxillofacial organoids. Then, we detail the use of modified co-culture methods, such as cell cluster co-inoculation and air-liquid interface culture technology to reconstitute the vascular network and immune microenvironment in assembled organoids. We further retrospect the existing oral and maxillofacial assembled organoids and their potential to recapitulate the homeostasis in parental tissues such as tooth, salivary gland, and mucosa. Finally, we discuss how the next-generation organoids may benefit to regenerative and precision medicine for treatment of oral-maxillofacial illness.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":10.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Personalized bioceramic grafts for craniomaxillofacial bone regeneration 用于颅颌面骨再生的个性化生物陶瓷移植物
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2024-10-31 DOI: 10.1038/s41368-024-00327-7
Ana Beatriz G. de Carvalho, Maedeh Rahimnejad, Rodrigo L. M. S. Oliveira, Prabaha Sikder, Guilherme S. F. A. Saavedra, Sarit B. Bhaduri, Debby Gawlitta, Jos Malda, Darnell Kaigler, Eliandra S. Trichês, Marco C. Bottino
{"title":"Personalized bioceramic grafts for craniomaxillofacial bone regeneration","authors":"Ana Beatriz G. de Carvalho, Maedeh Rahimnejad, Rodrigo L. M. S. Oliveira, Prabaha Sikder, Guilherme S. F. A. Saavedra, Sarit B. Bhaduri, Debby Gawlitta, Jos Malda, Darnell Kaigler, Eliandra S. Trichês, Marco C. Bottino","doi":"10.1038/s41368-024-00327-7","DOIUrl":"https://doi.org/10.1038/s41368-024-00327-7","url":null,"abstract":"<p>The reconstruction of craniomaxillofacial bone defects remains clinically challenging. To date, autogenous grafts are considered the gold standard but present critical drawbacks. These shortcomings have driven recent research on craniomaxillofacial bone reconstruction to focus on synthetic grafts with distinct materials and fabrication techniques. Among the various fabrication methods, additive manufacturing (AM) has shown significant clinical potential. AM technologies build three-dimensional (3D) objects with personalized geometry customizable from a computer-aided design. These layer-by-layer 3D biomaterial structures can support bone formation by guiding cell migration/proliferation, osteogenesis, and angiogenesis. Additionally, these structures can be engineered to degrade concomitantly with the new bone tissue formation, making them ideal as synthetic grafts. This review delves into the key advances of bioceramic grafts/scaffolds obtained by 3D printing for personalized craniomaxillofacial bone reconstruction. In this regard, clinically relevant topics such as ceramic-based biomaterials, graft/scaffold characteristics (macro/micro-features), material extrusion-based 3D printing, and the step-by-step workflow to engineer personalized bioceramic grafts are discussed. Importantly, in vitro models are highlighted in conjunction with a thorough examination of the signaling pathways reported when investigating these bioceramics and their effect on cellular response/behavior. Lastly, we summarize the clinical potential and translation opportunities of personalized bioceramics for craniomaxillofacial bone regeneration.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":14.9,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142555780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An unexpected role of neurite outgrowth inhibitor A as regulator of tooth enamel formation 神经细胞生长抑制剂 A 在牙釉质形成过程中发挥着意想不到的调节作用
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2024-10-20 DOI: 10.1038/s41368-024-00323-x
Pierfrancesco Pagella, Chai Foong Lai, Laurence Pirenne, Claudio Cantù, Martin E. Schwab, Thimios A. Mitsiadis
{"title":"An unexpected role of neurite outgrowth inhibitor A as regulator of tooth enamel formation","authors":"Pierfrancesco Pagella, Chai Foong Lai, Laurence Pirenne, Claudio Cantù, Martin E. Schwab, Thimios A. Mitsiadis","doi":"10.1038/s41368-024-00323-x","DOIUrl":"https://doi.org/10.1038/s41368-024-00323-x","url":null,"abstract":"<p>Neurite outgrowth inhibitor A (Nogo-A) is a major player in neural development and regeneration and the target of clinical trials aiming at promoting the regeneration of the central nervous system upon traumatic and ischemic injury. In this work, we investigated the functions of Nogo-A during tooth development to determine its role in dental physiology and pathology. Using immunohistochemistry and in situ hybridization techniques, we showed that Nogo-A is highly expressed in the developing mouse teeth and, most specifically, in the ameloblasts that are responsible for the formation of enamel. Using both <i>Nogo-A</i> knockout and <i>K14-Cre;Nogo-A fl/fl</i> transgenic mice, we showed that Nogo-A deletion in the dental epithelium leads to the formation of defective enamel. This phenotype is associated with overexpression of a set of specific genes involved in ameloblast differentiation and enamel matrix production, such as <i>amelogenin</i>, <i>ameloblastin</i> and <i>enamelin</i>. By characterising the interactome of Nogo-A in the dental epithelium of wild-type and mutant animals, we found that Nogo-A directly interacts with molecules important for regulating gene expression, and its deletion disturbs their cellular localisation. Furthermore, we demonstrated that inhibition of the intracellular, but not cell-surface, Nogo-A is responsible for gene expression modulation in ameloblasts. Taken together, these results reveal an unexpected function for Nogo-A in tooth enamel formation by regulating gene expression and cytodifferentiation events.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":14.9,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Periodontitis impacts on thrombotic diseases: from clinical aspect to future therapeutic approaches. 牙周炎对血栓性疾病的影响:从临床方面到未来的治疗方法。
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2024-10-15 DOI: 10.1038/s41368-024-00325-9
Jinting Ge,Xuanzhi Zhu,Chengxin Weng,Ding Yuan,Jichun Zhao,Lei Zhao,Tiehao Wang,Yafei Wu
{"title":"Periodontitis impacts on thrombotic diseases: from clinical aspect to future therapeutic approaches.","authors":"Jinting Ge,Xuanzhi Zhu,Chengxin Weng,Ding Yuan,Jichun Zhao,Lei Zhao,Tiehao Wang,Yafei Wu","doi":"10.1038/s41368-024-00325-9","DOIUrl":"https://doi.org/10.1038/s41368-024-00325-9","url":null,"abstract":"Periodontitis is a chronic inflammatory disease initiated by biofilm microorganisms and mediated by host immune imbalance. Uncontrolled periodontal infections are the leading cause of tooth loss in adults. Thrombotic diseases can lead to partial or complete obstruction of blood flow in the circulatory system, manifesting as organ or tissue ischemia and necrosis in patients with arterial thrombosis, and local edema, pain and circulatory instability in patients with venous thrombosis, which may lead to mortality or fatality in severe case. Recent studies found that periodontitis might enhance thrombosis through bacterial transmission or systemic inflammation by affecting platelet-immune cell interactions, as well as the coagulation, and periodontal therapy could have a prophylactic effect on patients with thrombotic diseases. In this review, we summarized clinical findings on the association between periodontitis and thrombotic diseases and discussed several novel prothrombotic periodontitis-related agents, and presented a perspective to emphasize the necessity of oral health management for people at high risk of thrombosis.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":14.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142439292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B. CREB3L1 缺乏会部分通过 TMEM30B 影响牙本质分化和磨牙牙本质沉积。
IF 10.8 1区 医学
International Journal of Oral Science Pub Date : 2024-10-10 DOI: 10.1038/s41368-024-00322-y
Yuanyuan Li, Yuxiu Lin, Jinqiang Guo, Delan Huang, Huanyan Zuo, Hanshu Zhang, Guohua Yuan, Huan Liu, Zhi Chen
{"title":"CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B.","authors":"Yuanyuan Li, Yuxiu Lin, Jinqiang Guo, Delan Huang, Huanyan Zuo, Hanshu Zhang, Guohua Yuan, Huan Liu, Zhi Chen","doi":"10.1038/s41368-024-00322-y","DOIUrl":"10.1038/s41368-024-00322-y","url":null,"abstract":"<p><p>Odontoblasts are primarily responsible for synthesizing and secreting extracellular matrix proteins, which are crucial for dentinogenesis. Our previous single-cell profile and RNAscope for odontoblast lineage revealed that cyclic adenosine monophosphate responsive element-binding protein 3 like 1 (Creb3l1) was specifically enriched in the terminal differentiated odontoblasts. In this study, deletion of Creb3l1 in the Wnt1+ lineage led to insufficient root elongation and dentin deposition. Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) and RNA sequencing were performed to revealed that in CREB3L1-deficient mouse dental papilla cells (mDPCs), the genes near the closed chromatin regions were mainly associated with mesenchymal development and the downregulated genes were primarily related to biological processes including cell differentiation, protein biosynthesis and transport, all of which were evidenced by a diminished ability of odontoblastic differentiation, a significant reduction in intracellular proteins, and an even greater decline in extracellular supernatant proteins. Dentin matrix protein 1 (Dmp1), dentin sialophosphoprotein (Dspp), and transmembrane protein 30B (Tmem30b) were identified as direct transcriptional regulatory targets. TMEM30B was intensively expressed in the differentiated odontoblasts, and exhibited a significant decline in both CREB3L1-deficient odontoblasts in vivo and in vitro. Deletion of Tmem30b impaired the ability of odontoblastic differentiation, protein synthesis, and protein secretion in mDPCs. Moreover, overexpressing TMEM30B in CREB3L1-deficient mDPCs partially rescued the extracellular proteins secretion. Collectively, our findings suggest that CREB3L1 participates in dentinogenesis and facilitates odontoblastic differentiation by directly enhancing the transcription of Dmp1, Dspp, and other differentiation-related genes and indirectly promoting protein secretion partially via TMEM30B.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":10.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experts consensus on management of tooth luxation and avulsion 专家就牙齿松动和脱落的处理达成共识
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2024-09-26 DOI: 10.1038/s41368-024-00321-z
Ruijie Huang, Chenchen Zhou, Ling Zhan, Yuan Liu, Xian Liu, Qin Du, Jun Wang, Wei Zhao, Guangtai Song, Li-an Wu, Beizhan Jiang, Yanhong Li, Hongmei Zhang, Jing Zou
{"title":"Experts consensus on management of tooth luxation and avulsion","authors":"Ruijie Huang, Chenchen Zhou, Ling Zhan, Yuan Liu, Xian Liu, Qin Du, Jun Wang, Wei Zhao, Guangtai Song, Li-an Wu, Beizhan Jiang, Yanhong Li, Hongmei Zhang, Jing Zou","doi":"10.1038/s41368-024-00321-z","DOIUrl":"https://doi.org/10.1038/s41368-024-00321-z","url":null,"abstract":"<p>Traumatic dental injuries (TDIs) of teeth occur frequently in children and adolescents. TDIs that impact the periodontal tissues and alveolar tissue can be classified into concussion, subluxation, extrusive luxation, intrusive luxation, lateral luxation, and avulsion. In these TDIs, management of injured soft tissue, mainly periodontal ligament, and dental pulp, is crucial in maintaining the function and longevity of the injured teeth. Factors that need to be considered for management in laxation injuries include the maturation stage of the traumatic teeth, mobility, direction of displacement, distance of displacement, and whether there are alveolar fractures. In avulsion, the maturation stage of the permanent tooth, the out-socket time, storage media/condition of the avulsed tooth, and management of the PDL should also be considered. Especially, in this review, we have subdivided the immature tooth into the adolescent tooth (Nolla stage 9) and the very young tooth (Nolla stage 8 and below). This consensus paper aimed to discuss the impacts of those factors on the trauma management and prognosis of TDI to provide a streamlined guide for clinicians from clinical evaluation, diagnostic process, management plan decision, follow-up, and orthodontic treatment for tooth luxation and avulsion injuries.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":14.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142321379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The circadian clock in enamel development 珐琅质发育过程中的昼夜节律时钟
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2024-09-06 DOI: 10.1038/s41368-024-00317-9
Ke Wu, Xiaochan Li, Yunyang Bai, Boon Chin Heng, Xuehui Zhang, Xuliang Deng
{"title":"The circadian clock in enamel development","authors":"Ke Wu, Xiaochan Li, Yunyang Bai, Boon Chin Heng, Xuehui Zhang, Xuliang Deng","doi":"10.1038/s41368-024-00317-9","DOIUrl":"https://doi.org/10.1038/s41368-024-00317-9","url":null,"abstract":"<p>Circadian rhythms are self-sustaining oscillations within biological systems that play key roles in a diverse multitude of physiological processes. The circadian clock mechanisms in brain and peripheral tissues can oscillate independently or be synchronized/disrupted by external stimuli. Dental enamel is a type of mineralized tissue that forms the exterior surface of the tooth crown. Incremental Retzius lines are readily observable microstructures of mature tooth enamel that indicate the regulation of amelogenesis by circadian rhythms. Teeth enamel is formed by enamel-forming cells known as ameloblasts, which are regulated and orchestrated by the circadian clock during amelogenesis. This review will first examine the key roles of the circadian clock in regulating ameloblasts and amelogenesis. Several physiological processes are involved, including gene expression, cell morphology, metabolic changes, matrix deposition, ion transportation, and mineralization. Next, the potential detrimental effects of circadian rhythm disruption on enamel formation are discussed. Circadian rhythm disruption can directly lead to Enamel Hypoplasia, which might also be a potential causative mechanism of amelogenesis imperfecta. Finally, future research trajectory in this field is extrapolated. It is hoped that this review will inspire more intensive research efforts and provide relevant cues in formulating novel therapeutic strategies for preventing tooth enamel developmental abnormalities.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":14.9,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expert consensus on the diagnosis and therapy of endo-periodontal lesions 牙周病内病变诊断与治疗专家共识
IF 14.9 1区 医学
International Journal of Oral Science Pub Date : 2024-09-01 DOI: 10.1038/s41368-024-00320-0
Bin Chen, Yanan Zhu, Minkui Lin, Yangheng Zhang, Yanfen Li, Xiangying Ouyang, Song Ge, Jiang Lin, Yaping Pan, Yan Xu, Yi Ding, Shaohua Ge, Faming Chen, Zhongchen Song, Shaoyun Jiang, Jiang Sun, Lijun Luo, Junqi Ling, Zhi Chen, Lin Yue, Xuedong Zhou, Fuhua Yan
{"title":"Expert consensus on the diagnosis and therapy of endo-periodontal lesions","authors":"Bin Chen, Yanan Zhu, Minkui Lin, Yangheng Zhang, Yanfen Li, Xiangying Ouyang, Song Ge, Jiang Lin, Yaping Pan, Yan Xu, Yi Ding, Shaohua Ge, Faming Chen, Zhongchen Song, Shaoyun Jiang, Jiang Sun, Lijun Luo, Junqi Ling, Zhi Chen, Lin Yue, Xuedong Zhou, Fuhua Yan","doi":"10.1038/s41368-024-00320-0","DOIUrl":"https://doi.org/10.1038/s41368-024-00320-0","url":null,"abstract":"<p>Endo-periodontal lesions (EPLs) involve both the periodontium and pulp tissue and have complicated etiologies and pathogenic mechanisms, including unique anatomical and microbiological characteristics and multiple contributing factors. This etiological complexity leads to difficulties in determining patient prognosis, posing great challenges in clinical practice. Furthermore, EPL-affected teeth require multidisciplinary therapy, including periodontal therapy, endodontic therapy and others, but there is still much debate about the appropriate timing of periodontal therapy and root canal therapy. By compiling the most recent findings on the etiology, pathogenesis, clinical characteristics, diagnosis, therapy, and prognosis of EPL-affected teeth, this consensus sought to support clinicians in making the best possible treatment decisions based on both biological and clinical evidence.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":14.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Caspase-11 mediated inflammasome activation in macrophages by systemic infection of A. actinomycetemcomitans exacerbates arthritis. 放线菌全身感染导致巨噬细胞中的 Caspase-11 介导的炎性体激活会加重关节炎。
IF 10.8 1区 医学
International Journal of Oral Science Pub Date : 2024-08-15 DOI: 10.1038/s41368-024-00315-x
Tokuju Okano, Hiroshi Ashida, Noriko Komatsu, Masayuki Tsukasaki, Tamako Iida, Marie Iwasawa, Yuto Takahashi, Yasuo Takeuchi, Takanori Iwata, Miwa Sasai, Masahiro Yamamoto, Hiroshi Takayanagi, Toshihiko Suzuki
{"title":"Caspase-11 mediated inflammasome activation in macrophages by systemic infection of A. actinomycetemcomitans exacerbates arthritis.","authors":"Tokuju Okano, Hiroshi Ashida, Noriko Komatsu, Masayuki Tsukasaki, Tamako Iida, Marie Iwasawa, Yuto Takahashi, Yasuo Takeuchi, Takanori Iwata, Miwa Sasai, Masahiro Yamamoto, Hiroshi Takayanagi, Toshihiko Suzuki","doi":"10.1038/s41368-024-00315-x","DOIUrl":"10.1038/s41368-024-00315-x","url":null,"abstract":"<p><p>Clinical studies have shown that Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is associated with aggressive periodontitis and can potentially trigger or exacerbate rheumatoid arthritis (RA). However, the mechanism is poorly understood. Here, we show that systemic infection with A. actinomycetemcomitans triggers the progression of arthritis in mice anti-collagen antibody-induced arthritis (CAIA) model following IL-1β secretion and cell infiltration in paws in a manner that is dependent on caspase-11-mediated inflammasome activation in macrophages. The administration of polymyxin B (PMB), chloroquine, and anti-CD11b antibody suppressed inflammasome activation in macrophages and arthritis in mice, suggesting that the recognition of lipopolysaccharide (LPS) in the cytosol after bacterial degradation by lysosomes and invasion via CD11b are needed to trigger arthritis following inflammasome activation in macrophages. These data reveal that the inhibition of caspase-11-mediated inflammasome activation potentiates aggravation of RA induced by infection with A. actinomycetemcomitans. This work highlights how RA can be progressed by inflammasome activation as a result of periodontitis-associated bacterial infection and discusses the mechanism of inflammasome activation in response to infection with A. actinomycetemcomitans.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":10.8,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11324795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gingipain from Porphyromonas gingivalis causes insulin resistance by degrading insulin receptors through direct proteolytic effects. 牙龈卟啉单胞菌中的 Gingipain 通过直接蛋白水解作用降解胰岛素受体,从而导致胰岛素抵抗。
IF 10.8 1区 医学
International Journal of Oral Science Pub Date : 2024-08-01 DOI: 10.1038/s41368-024-00313-z
Fen Liu, Bofeng Zhu, Ying An, Zhifei Zhou, Peiying Xiong, Xuan Li, Yang Mi, Tongqiang He, Faming Chen, Buling Wu
{"title":"Gingipain from Porphyromonas gingivalis causes insulin resistance by degrading insulin receptors through direct proteolytic effects.","authors":"Fen Liu, Bofeng Zhu, Ying An, Zhifei Zhou, Peiying Xiong, Xuan Li, Yang Mi, Tongqiang He, Faming Chen, Buling Wu","doi":"10.1038/s41368-024-00313-z","DOIUrl":"10.1038/s41368-024-00313-z","url":null,"abstract":"<p><p>Periodontitis is a critical risk factor for the occurrence and development of diabetes. Porphyromonas gingivalis may participate in insulin resistance (IR) caused by periodontal inflammation, but the functional role and specific mechanisms of P. gingivalis in IR remain unclear. In the present study, clinical samples were analysed to determine the statistical correlation between P. gingivalis and IR occurrence. Through culturing of hepatocytes, myocytes, and adipocytes, and feeding mice P. gingivalis orally, the functional correlation between P. gingivalis and IR occurrence was further studied both in vitro and in vivo. Clinical data suggested that the amount of P. gingivalis isolated was correlated with the Homeostatic Model Assessment for IR score. In vitro studies suggested that coculture with P. gingivalis decreased glucose uptake and insulin receptor (INSR) protein expression in hepatocytes, myocytes, and adipocytes. Mice fed P. gingivalis tended to undergo IR. P. gingivalis was detectable in the liver, skeletal muscle, and adipose tissue of experimental mice. The distribution sites of gingipain coincided with the downregulation of INSR. Gingipain proteolysed the functional insulin-binding region of INSR. Coculture with P. gingivalis significantly decreased the INSR-insulin binding ability. Knocking out gingipain from P. gingivalis alleviated the negative effects of P. gingivalis on IR in vivo. Taken together, these findings indicate that distantly migrated P. gingivalis may directly proteolytically degrade INSR through gingipain, thereby leading to IR. The results provide a new strategy for preventing diabetes by targeting periodontal pathogens and provide new ideas for exploring novel mechanisms by which periodontal inflammation affects the systemic metabolic state.</p>","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":null,"pages":null},"PeriodicalIF":10.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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