International Journal of Oral Science最新文献_第10页

Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing. 单细胞RNA测序技术在龋齿牙髓组织细胞外基质重构中的应用。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-08-02 DOI: 10.1038/s41368-023-00238-z

Anamaria Balic, Dilara Perver, Pierfrancesco Pagella, Hubert Rehrauer, Bernd Stadlinger, Andreas E Moor, Viola Vogel, Thimios A Mitsiadis

{"title":"Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing.","authors":"Anamaria Balic, Dilara Perver, Pierfrancesco Pagella, Hubert Rehrauer, Bernd Stadlinger, Andreas E Moor, Viola Vogel, Thimios A Mitsiadis","doi":"10.1038/s41368-023-00238-z","DOIUrl":"https://doi.org/10.1038/s41368-023-00238-z","url":null,"abstract":"Carious lesions are bacteria-caused destructions of the mineralised dental tissues, marked by the simultaneous activation of immune responses and regenerative events within the soft dental pulp tissue. While major molecular players in tooth decay have been uncovered during the past years, a detailed map of the molecular and cellular landscape of the diseased pulp is still missing. In this study we used single-cell RNA sequencing analysis, supplemented with immunostaining, to generate a comprehensive single-cell atlas of the pulp of carious human teeth. Our data demonstrated modifications in the various cell clusters within the pulp of carious teeth, such as immune cells, mesenchymal stem cells (MSC) and fibroblasts, when compared to the pulp of healthy human teeth. Active immune response in the carious pulp tissue is accompanied by specific changes in the fibroblast and MSC clusters. These changes include the upregulation of genes encoding extracellular matrix (ECM) components, including COL1A1 and Fibronectin (FN1), and the enrichment of the fibroblast cluster with myofibroblasts. The incremental changes in the ECM composition of carious pulp tissues were further confirmed by immunostaining analyses. Assessment of the Fibronectin fibres under mechanical strain conditions showed a significant tension reduction in carious pulp tissues, compared to the healthy ones. The present data demonstrate molecular, cellular and biomechanical alterations in the pulp of human carious teeth, indicative of extensive ECM remodelling, reminiscent of fibrosis observed in other organs. This comprehensive atlas of carious human teeth can facilitate future studies of dental pathologies and enable comparative analyses across diseased organs.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"30"},"PeriodicalIF":14.9,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10397277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9941374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of dendritic cells in MYD88-mediated immune recognition and osteoinduction initiated by the implantation of biomaterials. 树突状细胞在myd88介导的免疫识别和生物材料植入引发的骨诱导中的作用。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-08-02 DOI: 10.1038/s41368-023-00234-3

Zifan Zhao, Qin Zhao, Hu Chen, Fanfan Chen, Feifei Wang, Hua Tang, Haibin Xia, Yongsheng Zhou, Yuchun Sun

{"title":"Role of dendritic cells in MYD88-mediated immune recognition and osteoinduction initiated by the implantation of biomaterials.","authors":"Zifan Zhao, Qin Zhao, Hu Chen, Fanfan Chen, Feifei Wang, Hua Tang, Haibin Xia, Yongsheng Zhou, Yuchun Sun","doi":"10.1038/s41368-023-00234-3","DOIUrl":"https://doi.org/10.1038/s41368-023-00234-3","url":null,"abstract":"Bone substitute material implantation has become an important treatment strategy for the repair of oral and maxillofacial bone defects. Recent studies have shown that appropriate inflammatory and immune cells are essential factors in the process of osteoinduction of bone substitute materials. Previous studies have mainly focused on innate immune cells such as macrophages. In our previous work, we found that T lymphocytes, as adaptive immune cells, are also essential in the osteoinduction procedure. As the most important antigen-presenting cell, whether dendritic cells (DCs) can recognize non-antigen biomaterials and participate in osteoinduction was still unclear. In this study, we found that surgical trauma associated with materials implantation induces necrocytosis, and this causes the release of high mobility group protein-1 (HMGB1), which is adsorbed on the surface of bone substitute materials. Subsequently, HMGB1-adsorbed materials were recognized by the TLR4-MYD88-NFκB signal axis of dendritic cells, and the inflammatory response was activated. Finally, activated DCs release regeneration-related chemokines, recruit mesenchymal stem cells, and initiate the osteoinduction process. This study sheds light on the immune-regeneration process after bone substitute materials implantation, points out a potential direction for the development of bone substitute materials, and provides guidance for the development of clinical surgical methods.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"31"},"PeriodicalIF":14.9,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10397189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9941379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling. 癌相关成纤维细胞衍生的赖氨酸氧化酶丰富的细胞外囊泡介导胶原交联，并通过p-FAK/p-paxillin/YAP信号通路促进上皮-间质转化。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-08-02 DOI: 10.1038/s41368-023-00236-1

Xue Liu, Jiao Li, Xuesong Yang, Xiaojie Li, Jing Kong, Dongyuan Qi, Fuyin Zhang, Bo Sun, Yuehua Liu, Tingjiao Liu

{"title":"Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling.","authors":"Xue Liu, Jiao Li, Xuesong Yang, Xiaojie Li, Jing Kong, Dongyuan Qi, Fuyin Zhang, Bo Sun, Yuehua Liu, Tingjiao Liu","doi":"10.1038/s41368-023-00236-1","DOIUrl":"https://doi.org/10.1038/s41368-023-00236-1","url":null,"abstract":"Carcinoma-associated fibroblasts (CAFs) are the main cellular components of the tumor microenvironment and promote cancer progression by modifying the extracellular matrix (ECM). The tumor-associated ECM is characterized by collagen crosslinking catalyzed by lysyl oxidase (LOX). Small extracellular vesicles (sEVs) mediate cell-cell communication. However, the interactions between sEVs and the ECM remain unclear. Here, we demonstrated that sEVs released from oral squamous cell carcinoma (OSCC)-derived CAFs induce collagen crosslinking, thereby promoting epithelial-mesenchymal transition (EMT). CAF sEVs preferably bound to the ECM rather than being taken up by fibroblasts and induced collagen crosslinking, and a LOX inhibitor or blocking antibody suppressed this effect. Active LOX (αLOX), but not the LOX precursor, was enriched in CAF sEVs and interacted with periostin, fibronectin, and bone morphogenetic protein-1 on the surface of sEVs. CAF sEV-associated integrin α2β1 mediated the binding of CAF sEVs to collagen I, and blocking integrin α2β1 inhibited collagen crosslinking by interfering with CAF sEV binding to collagen I. CAF sEV-induced collagen crosslinking promoted the EMT of OSCC through FAK/paxillin/YAP pathway. Taken together, these findings reveal a novel role of CAF sEVs in tumor ECM remodeling, suggesting a critical mechanism for CAF-induced EMT of cancer cells.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"32"},"PeriodicalIF":14.9,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10397209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9935269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ChatGPT for shaping the future of dentistry: the potential of multi-modal large language model. 打造牙科未来的 ChatGPT：多模态大语言模型的潜力。

IF 10.8 1区医学

International Journal of Oral Science Pub Date : 2023-07-28 DOI: 10.1038/s41368-023-00239-y

Hanyao Huang, Ou Zheng, Dongdong Wang, Jiayi Yin, Zijin Wang, Shengxuan Ding, Heng Yin, Chuan Xu, Renjie Yang, Qian Zheng, Bing Shi

{"title":"ChatGPT for shaping the future of dentistry: the potential of multi-modal large language model.","authors":"Hanyao Huang, Ou Zheng, Dongdong Wang, Jiayi Yin, Zijin Wang, Shengxuan Ding, Heng Yin, Chuan Xu, Renjie Yang, Qian Zheng, Bing Shi","doi":"10.1038/s41368-023-00239-y","DOIUrl":"10.1038/s41368-023-00239-y","url":null,"abstract":"The ChatGPT, a lite and conversational variant of Generative Pretrained Transformer 4 (GPT-4) developed by OpenAI, is one of the milestone Large Language Models (LLMs) with billions of parameters. LLMs have stirred up much interest among researchers and practitioners in their impressive skills in natural language processing tasks, which profoundly impact various fields. This paper mainly discusses the future applications of LLMs in dentistry. We introduce two primary LLM deployment methods in dentistry, including automated dental diagnosis and cross-modal dental diagnosis, and examine their potential applications. Especially, equipped with a cross-modal encoder, a single LLM can manage multi-source data and conduct advanced natural language reasoning to perform complex clinical operations. We also present cases to demonstrate the potential of a fully automatic Multi-Modal LLM AI system for dentistry clinical application. While LLMs offer significant potential benefits, the challenges, such as data privacy, data quality, and model bias, need further study. Overall, LLMs have the potential to revolutionize dental diagnosis and treatment, which indicates a promising avenue for clinical application and research in dentistry.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"29"},"PeriodicalIF":10.8,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9927224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minimally invasive techniques for lateral maxillary sinus floor elevation: small lateral window and one-stage surgery-a 2-5-year retrospective study. 上颌外侧窦底抬高的微创技术:小外侧窗口和一期手术-一项2-5年回顾性研究。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-07-11 DOI: 10.1038/s41368-023-00233-4

Shaojingya Gao, Yao Jiang, Yangxue Yao, Songhang Li, Xiaoxiao Cai

{"title":"Minimally invasive techniques for lateral maxillary sinus floor elevation: small lateral window and one-stage surgery-a 2-5-year retrospective study.","authors":"Shaojingya Gao, Yao Jiang, Yangxue Yao, Songhang Li, Xiaoxiao Cai","doi":"10.1038/s41368-023-00233-4","DOIUrl":"https://doi.org/10.1038/s41368-023-00233-4","url":null,"abstract":"This study aimed to introduce a minimally invasive technique for maxillary sinus floor elevation using the lateral approach (lSFE) and to determine the factors that influence the stability of the grafted area in the sinus cavity. Thirty patients (30 implants) treated with lSFE using minimally invasive techniques from 2015 to 2019 were included in the study. Five aspects of the implant (central, mesial, distal, buccal, and palatal bone heights [BHs]) were measured using cone-beam computed tomography (CBCT) before implant surgery, immediately after surgery (T0), 6 months after surgery (T1), and at the last follow-up visit (T2). Patients' characteristics were collected. A small bone window (height, (4.40 ± 0.74) mm; length, (6.26 ± 1.03) mm) was prepared. No implant failed during the follow-up period (3.67 ± 1.75) years. Three of the 30 implants exhibited perforations. Changes in BH of the five aspects of implants showed strong correlations with each other and BH decreased dramatically before second-stage surgery. Residual bone height (RBH) did not significantly influence BH changes, whereas smoking status and type of bone graft materials were the potentially influential factors. During the approximate three-year observation period, lSFE with a minimally invasive technique demonstrated high implant survival rate and limited bone reduction in grafted area. In conclusion, lSFE using minimally invasive techniques was a viable treatment option. Patients who were nonsmokers and whose sinus cavity was filled with deproteinized bovine bone mineral (DBBM) had significantly limited bone resorption in grafted area.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"28"},"PeriodicalIF":14.9,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9826246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salivary biomarkers: novel noninvasive tools to diagnose chronic inflammation. 唾液生物标志物:诊断慢性炎症的新型无创工具。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-06-29 DOI: 10.1038/s41368-023-00231-6

Paola Dongiovanni, Marica Meroni, Sara Casati, Riccardo Goldoni, Douglas Vieira Thomaz, Nermin Seda Kehr, Daniela Galimberti, Massimo Del Fabbro, Gianluca M Tartaglia

{"title":"Salivary biomarkers: novel noninvasive tools to diagnose chronic inflammation.","authors":"Paola Dongiovanni, Marica Meroni, Sara Casati, Riccardo Goldoni, Douglas Vieira Thomaz, Nermin Seda Kehr, Daniela Galimberti, Massimo Del Fabbro, Gianluca M Tartaglia","doi":"10.1038/s41368-023-00231-6","DOIUrl":"https://doi.org/10.1038/s41368-023-00231-6","url":null,"abstract":"Several chronic disorders including type 2 diabetes (T2D), obesity, heart disease and cancer are preceded by a state of chronic low-grade inflammation. Biomarkers for the early assessment of chronic disorders encompass acute phase proteins (APP), cytokines and chemokines, pro-inflammatory enzymes, lipids and oxidative stress mediators. These substances enter saliva through the blood flow and, in some cases, there is a close relation between their salivary and serum concentration. Saliva can be easily collected and stored with non-invasive and cost-saving procedures, and it is emerging the concept to use it for the detection of inflammatory biomarkers. To this purpose, the present review aims to discuss the advantages and challenges of using standard and cutting-edge techniques to discover salivary biomarkers which may be used in diagnosis/therapy of several chronic diseases with inflammatory consequences with the pursuit to possibly replace conventional paths with detectable soluble mediators in saliva. Specifically, the review describes the procedures used for saliva collection, the standard approaches for the measurement of salivary biomarkers and the novel methodological strategies such as biosensors to improve the quality of care for chronically affected patients.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"27"},"PeriodicalIF":14.9,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9734869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Oral pathogen aggravates atherosclerosis by inducing smooth muscle cell apoptosis and repressing macrophage efferocytosis. 口腔病原体通过诱导平滑肌细胞凋亡和抑制巨噬细胞efferocysis而加重动脉粥样硬化。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-06-28 DOI: 10.1038/s41368-023-00232-5

Hanyu Xie, Ziyue Qin, Ziji Ling, Xiao Ge, Hang Zhang, Shuyu Guo, Laikui Liu, Kai Zheng, Hongbing Jiang, Rongyao Xu

{"title":"Oral pathogen aggravates atherosclerosis by inducing smooth muscle cell apoptosis and repressing macrophage efferocytosis.","authors":"Hanyu Xie, Ziyue Qin, Ziji Ling, Xiao Ge, Hang Zhang, Shuyu Guo, Laikui Liu, Kai Zheng, Hongbing Jiang, Rongyao Xu","doi":"10.1038/s41368-023-00232-5","DOIUrl":"https://doi.org/10.1038/s41368-023-00232-5","url":null,"abstract":"Periodontitis imparting the increased risk of atherosclerotic cardiovascular diseases is partially due to the immune subversion of the oral pathogen, particularly the Porphyromonas gingivalis (P. gingivalis), by inducing apoptosis. However, it remains obscure whether accumulated apoptotic cells in P. gingivalis-accelerated plaque formation are associated with impaired macrophage clearance. Here, we show that smooth muscle cells (SMCs) have a greater susceptibility to P. gingivalis-induced apoptosis than endothelial cells through TLR2 pathway activation. Meanwhile, large amounts of miR-143/145 in P.gingivalis-infected SMCs are extracellularly released and captured by macrophages. Then, these miR-143/145 are translocated into the nucleus to promote Siglec-G transcription, which represses macrophage efferocytosis. By constructing three genetic mouse models, we further confirm the in vivo roles of TLR2 and miR-143/145 in P. gingivalis-accelerated atherosclerosis. Therapeutically, we develop P.gingivalis-pretreated macrophage membranes to coat metronidazole and anti-Siglec-G antibodies for treating atherosclerosis and periodontitis simultaneously. Our findings extend the knowledge of the mechanism and therapeutic strategy in oral pathogen-associated systemic diseases.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"26"},"PeriodicalIF":14.9,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9742803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodontitis as a promoting factor of T2D: current evidence and mechanisms. 牙周炎作为T2D的促进因素:目前的证据和机制。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-06-15 DOI: 10.1038/s41368-023-00227-2

Yuan Su, Leilei Ye, Chen Hu, Yanli Zhang, Jia Liu, Longquan Shao

{"title":"Periodontitis as a promoting factor of T2D: current evidence and mechanisms.","authors":"Yuan Su, Leilei Ye, Chen Hu, Yanli Zhang, Jia Liu, Longquan Shao","doi":"10.1038/s41368-023-00227-2","DOIUrl":"https://doi.org/10.1038/s41368-023-00227-2","url":null,"abstract":"Periodontitis is an infectious disease caused by an imbalance between the local microbiota and host immune response. Epidemiologically, periodontitis is closely related to the occurrence, development, and poor prognosis of T2D and is recognized as a potential risk factor for T2D. In recent years, increasing attention has been given to the role of the virulence factors produced by disorders of the subgingival microbiota in the pathological mechanism of T2D, including islet β-cell dysfunction and insulin resistance (IR). However, the related mechanisms have not been well summarized. This review highlights periodontitis-derived virulence factors, reviews how these stimuli directly or indirectly regulate islet β-cell dysfunction. The mechanisms by which IR is induced in insulin-targeting tissues (the liver, visceral adipose tissue, and skeletal muscle) are explained, clarifying the influence of periodontitis on the occurrence and development of T2D. In addition, the positive effects of periodontal therapy on T2D are overviewed. Finally, the limitations and prospects of the current research are discussed. In summary, periodontitis is worthy of attention as a promoting factor of T2D. Understanding on the effect of disseminated periodontitis-derived virulence factors on the T2D-related tissues and cells may provide new treatment options for reducing the risk of T2D associated with periodontitis.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"25"},"PeriodicalIF":14.9,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9658461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma. 在头颈部鳞状细胞癌中，LIMP-2通过促进自噬诱导的GSK3β降解增强癌症干样细胞特性。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-06-08 DOI: 10.1038/s41368-023-00229-0

Yuantong Liu, Shujin Li, Shuo Wang, Qichao Yang, Zhizhong Wu, Mengjie Zhang, Lei Chen, Zhijun Sun

{"title":"LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma.","authors":"Yuantong Liu, Shujin Li, Shuo Wang, Qichao Yang, Zhizhong Wu, Mengjie Zhang, Lei Chen, Zhijun Sun","doi":"10.1038/s41368-023-00229-0","DOIUrl":"10.1038/s41368-023-00229-0","url":null,"abstract":"Cancer stem cell-like cells (CSCs) play an integral role in the heterogeneity, metastasis, and treatment resistance of head and neck squamous cell carcinoma (HNSCC) due to their high tumor initiation capacity and plasticity. Here, we identified a candidate gene named LIMP-2 as a novel therapeutic target regulating HNSCC progression and CSC properties. The high expression of LIMP-2 in HNSCC patients suggested a poor prognosis and potential immunotherapy resistance. Functionally, LIMP-2 can facilitate autolysosome formation to promote autophagic flux. LIMP-2 knockdown inhibits autophagic flux and reduces the tumorigenic ability of HNSCC. Further mechanistic studies suggest that enhanced autophagy helps HNSCC maintain stemness and promotes degradation of GSK3β, which in turn facilitates nuclear translocation of β-catenin and transcription of downstream target genes. In conclusion, this study reveals LIMP-2 as a novel prospective therapeutic target for HNSCC and provides evidence for a link between autophagy, CSC, and immunotherapy resistance.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"24"},"PeriodicalIF":14.9,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9671457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Promotion effect of TGF-β-Zfp423-ApoD pathway on lip sensory recovery after nerve sacrifice caused by nerve collateral compensation. TGF-β-Zfp423-ApoD通路对神经侧支代偿所致神经牺牲后唇部感觉恢复的促进作用。

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2023-06-08 DOI: 10.1038/s41368-023-00230-7

Pingchuan Ma, Gaowei Zhang, Su Chen, Cheng Miao, Yubin Cao, Meng Wang, Wenwen Liu, Jiefei Shen, Patrick Ming-Kuen Tang, Yi Men, Li Ye, Chunjie Li

{"title":"Promotion effect of TGF-β-Zfp423-ApoD pathway on lip sensory recovery after nerve sacrifice caused by nerve collateral compensation.","authors":"Pingchuan Ma, Gaowei Zhang, Su Chen, Cheng Miao, Yubin Cao, Meng Wang, Wenwen Liu, Jiefei Shen, Patrick Ming-Kuen Tang, Yi Men, Li Ye, Chunjie Li","doi":"10.1038/s41368-023-00230-7","DOIUrl":"https://doi.org/10.1038/s41368-023-00230-7","url":null,"abstract":"Resection of oral and maxillofacial tumors is often accompanied by the inferior alveolar nerve neurectomy, resulting in abnormal sensation in lower lip. It is generally believed that spontaneous sensory recovery in this nerve injury is difficult. However, during our follow-up, patients with inferior alveolar nerve sacrifice showed different degrees of lower lip sensory recovery. In this study, a prospective cohort study was conducted to demonstrate this phenomenon and analyze the factors influencing sensory recovery. A mental nerve transection model of Thy1-YFP mice and tissue clearing technique were used to explore possible mechanisms in this process. Gene silencing and overexpression experiments were then conducted to detect the changes in cell morphology and molecular markers. In our follow-up, 75% of patients with unilateral inferior alveolar nerve neurectomy had complete sensory recovery of the lower lip 12 months postoperatively. Patients with younger age, malignant tumors, and preservation of ipsilateral buccal and lingual nerves had a shorter recovery time. The buccal nerve collateral sprouting compensation was observed in the lower lip tissue of Thy1-YFP mice. ApoD was demonstrated to be involved in axon growth and peripheral nerve sensory recovery in the animal model. TGF-β inhibited the expression of STAT3 and the transcription of ApoD in Schwann cells through Zfp423. Overall, after sacrificing the inferior alveolar nerve, the collateral compensation of the ipsilateral buccal nerve could innervate the sensation. And this process was regulated by TGF-β-Zfp423-ApoD pathway.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"15 1","pages":"23"},"PeriodicalIF":14.9,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9954516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0