International Journal of Oral Science最新文献_第4页

Expert consensus on imaging diagnosis and analysis of early correction of childhood malocclusion 儿童错牙合的影像学诊断及早期矫治分析专家共识

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-04-01 DOI: 10.1038/s41368-025-00351-1

Zitong Lin, Chenchen Zhou, Ziyang Hu, Zuyan Zhang, Yong Cheng, Bing Fang, Hong He, Hu Wang, Gang Li, Jun Guo, Weihua Guo, Xiaobing Li, Guangning Zheng, Zhimin Li, Donglin Zeng, Yan Liu, Yuehua Liu, Min Hu, Lunguo Xia, Jihong Zhao, Yaling Song, Huang Li, Jun Ji, Jinlin Song, Lili Chen, Tiemei Wang

引用次数: 0

Macrophage ATF6 accelerates corticotomy-assisted orthodontic tooth movement through promoting Tnfα transcription 巨噬细胞ATF6通过促进Tnfα转录加速皮质切开术辅助正畸牙齿运动

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-04-01 DOI: 10.1038/s41368-025-00359-7

Zhichun Jin, Hao Xu, Weiye Zhao, Kejia Zhang, Shengnan Wu, Chuanjun Shu, Linlin Zhu, Yan Wang, Lin Wang, Hanwen Zhang, Bin Yan

{"title":"Macrophage ATF6 accelerates corticotomy-assisted orthodontic tooth movement through promoting Tnfα transcription","authors":"Zhichun Jin, Hao Xu, Weiye Zhao, Kejia Zhang, Shengnan Wu, Chuanjun Shu, Linlin Zhu, Yan Wang, Lin Wang, Hanwen Zhang, Bin Yan","doi":"10.1038/s41368-025-00359-7","DOIUrl":"https://doi.org/10.1038/s41368-025-00359-7","url":null,"abstract":"Corticotomy is a clinical procedure to accelerate orthodontic tooth movement characterized by the regional acceleratory phenomenon (RAP). Despite its therapeutic effects, the surgical risk and unclear mechanism hamper the clinical application. Numerous evidences support macrophages as the key immune cells during bone remodeling. Our study discovered that the monocyte-derived macrophages primarily exhibited a pro-inflammatory phenotype that dominated bone remodeling in corticotomy by CX3CR1CreERT2; R26GFP lineage tracing system. Fluorescence staining, flow cytometry analysis, and western blot determined the significantly enhanced expression of binding immunoglobulin protein (BiP) and emphasized the activation of sensor activating transcription factor 6 (ATF6) in macrophages. Then, we verified that macrophage specific ATF6 deletion (ATF6f/f; CX3CR1CreERT2 mice) decreased the proportion of pro-inflammatory macrophages and therefore blocked the acceleration effect of corticotomy. In contrast, macrophage ATF6 overexpression exaggerated the acceleration of orthodontic tooth movement. In vitro experiments also proved that higher proportion of pro-inflammatory macrophages was positively correlated with higher expression of ATF6. At the mechanism level, RNA-seq and CUT&Tag analysis demonstrated that ATF6 modulated the macrophage-orchestrated inflammation through interacting with Tnfα promotor and augmenting its transcription. Additionally, molecular docking simulation and dual-luciferase reporter system indicated the possible binding sites outside of the traditional endoplasmic reticulum-stress response element (ERSE). Taken together, ATF6 may aggravate orthodontic bone remodeling by promoting Tnfα transcription in macrophages, suggesting that ATF6 may represent a promising therapeutic target for non-invasive accelerated orthodontics.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"58 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma 作者更正：LIMP-2通过促进头颈部鳞状细胞癌中自噬诱导的GSK3β降解来增强癌症干细胞样细胞的特性

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-28 DOI: 10.1038/s41368-025-00358-8

Yuantong Liu, Shujin Li, Shuo Wang, Qichao Yang, Zhizhong Wu, Mengjie Zhang, Lei Chen, Zhijun Sun

引用次数: 0

Abnormal collagen deposition mediated by cartilage oligomeric matrix protein in the pathogenesis of oral submucous fibrosis 软骨寡聚基质蛋白介导的异常胶原沉积在口腔黏膜下纤维化发病机制中的作用

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-27 DOI: 10.1038/s41368-025-00355-x

Yafei Xiong, Xuechun Li, Bincan Sun, Jie Zhang, Xiaoshan Wu, Feng Guo

{"title":"Abnormal collagen deposition mediated by cartilage oligomeric matrix protein in the pathogenesis of oral submucous fibrosis","authors":"Yafei Xiong, Xuechun Li, Bincan Sun, Jie Zhang, Xiaoshan Wu, Feng Guo","doi":"10.1038/s41368-025-00355-x","DOIUrl":"https://doi.org/10.1038/s41368-025-00355-x","url":null,"abstract":"Abnormal accumulation of collagen fibrils is a hallmark feature of oral submucous fibrosis (OSF). However, the precise characteristics and underlying mechanisms remain unclear, impeding the advancement of potential therapeutic approaches. Here, we observed that collagen I, the main component of the extracellular matrix, first accumulated in the lamina propria and subsequently in the submucosa of OSF specimens as the disease progressed. Using RNA-seq and Immunofluorescence in OSF specimens, we screened the cartilage oligomeric matrix protein (COMP) responsible for the abnormal collagen accumulation. Genetic COMP deficiency reduced arecoline-stimulated collagen I deposition significantly in vivo. In comparison, both COMP and collagen I were upregulated under arecoline stimulation in wild-type mice. Human oral buccal mucosal fibroblasts (hBMFs) also exhibited increased secretion of COMP and collagen I after stimulation in vitro. COMP knockdown in hBMFs downregulates arecoline-stimulated collagen I secretion. We further demonstrated that hBMFs present heterogeneous responses to arecoline stimulation, of which COMP-positive fibroblasts secrete more collagen I. Since COMP is a molecular bridge with Fibril-associated collagens with Interrupted Triple helices (FACIT) in the collagen network, we further screened and identified collagen XIV, a FACIT member, co-localizing with both COMP and collagen I. Collagen XIV expression increased under arecoline stimulation in wild-type mice, whereas it was hardly expressed in the Comp-/- mice, even with under stimulation. In summary, we found that COMP may mediates abnormal collagen I deposition by functions with collagen XIV during the progression of OSF, suggesting its potential to be targeted in treating OSF.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"88 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role and mechanisms of histone methylation in osteogenic/odontogenic differentiation of dental mesenchymal stem cells 组蛋白甲基化在牙间充质干细胞成骨/成牙分化中的作用和机制

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-26 DOI: 10.1038/s41368-025-00353-z

Meijun Hu, Zhipeng Fan

{"title":"Role and mechanisms of histone methylation in osteogenic/odontogenic differentiation of dental mesenchymal stem cells","authors":"Meijun Hu, Zhipeng Fan","doi":"10.1038/s41368-025-00353-z","DOIUrl":"https://doi.org/10.1038/s41368-025-00353-z","url":null,"abstract":"Dental mesenchymal stem cells (DMSCs) are pivotal for tooth development and periodontal tissue health and play an important role in tissue engineering and regenerative medicine because of their multidirectional differentiation potential and self-renewal ability. The cellular microenvironment regulates the fate of stem cells and can be modified using various optimization techniques. These methods can influence the cellular microenvironment, activate disparate signaling pathways, and induce different biological effects. “Epigenetic regulation” refers to the process of influencing gene expression and regulating cell fate without altering DNA sequences, such as histone methylation. Histone methylation modifications regulate pivotal transcription factors governing DMSCs differentiation into osteo-/odontogenic lineages. The most important sites of histone methylation in tooth organization were found to be H3K4, H3K9, and H3K27. Histone methylation affects gene expression and regulates stem cell differentiation by maintaining a delicate balance between major trimethylation sites, generating distinct chromatin structures associated with specific downstream transcriptional states. Several crucial signaling pathways associated with osteogenic differentiation are susceptible to modulation via histone methylation modifications. A deeper understanding of the regulatory mechanisms governing histone methylation modifications in osteo-/odontogenic differentiation and immune-inflammatory responses of DMSCs will facilitate further investigation of the epigenetic regulation of histone methylation in DMSC-mediated tissue regeneration and inflammation. Here is a concise overview of the pivotal functions of epigenetic histone methylation at H3K4, H3K9, and H3K27 in the regulation of osteo-/odontogenic differentiation and renewal of DMSCs in both non-inflammatory and inflammatory microenvironments. This review summarizes the current research on these processes in the context of tissue regeneration and therapeutic interventions.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"61 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of tricellular tight junction tricellulin leads to hyposalivation in Sjögren’s syndrome 三细胞紧密连接蛋白的缺失导致Sjögren综合征的低唾液分泌

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-19 DOI: 10.1038/s41368-025-00349-9

Xiangdi Mao, Haibing Li, Sainan Min, Jiazeng Su, Pan Wei, Yan Zhang, Qihua He, Liling Wu, Guangyan Yu, Xin Cong

{"title":"Loss of tricellular tight junction tricellulin leads to hyposalivation in Sjögren’s syndrome","authors":"Xiangdi Mao, Haibing Li, Sainan Min, Jiazeng Su, Pan Wei, Yan Zhang, Qihua He, Liling Wu, Guangyan Yu, Xin Cong","doi":"10.1038/s41368-025-00349-9","DOIUrl":"https://doi.org/10.1038/s41368-025-00349-9","url":null,"abstract":"Tricellulin, a key tricellular tight junction (TJ) protein, is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands. This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome (SS). Employing a multifaceted approach involving patient biopsies, non-obese diabetic (NOD) mice as a SS model, salivary gland acinar cell-specific tricellulin conditional knockout (TricCKO) mice, and IFN-γ-stimulated salivary gland epithelial cells, we investigated the role of tricellulin in SS-related hyposalivation. Our data revealed diminished levels of tricellulin in salivary glands of SS patients. Similarly, NOD mice displayed a reduction in tricellulin expression from the onset of the disease, concomitant with hyposecretion and an increase in salivary albumin content. Consistent with these findings, TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals. Mechanistically, the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin. Treatment with AT1001, a TJ sealer, ameliorated epithelial barrier dysfunction, restored tricellulin expression, and consequently alleviated hyposalivation in NOD mice. Importantly, treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage. Collectively, we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS. Our findings highlight tricellulin as a potential therapeutic target for hyposecretion, particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"17 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring 妊娠小鼠服用牙龈卟啉单胞菌可促进糖酵解和组蛋白乳酸化/ADAM17，导致后代腭裂

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-13 DOI: 10.1038/s41368-025-00347-x

Xige Zhao, Xiaoyu Zheng, Yijia Wang, Jing Chen, Xiaotong Wang, Xia Peng, Dong Yuan, Ying Liu, Zhiwei Wang, Juan Du

{"title":"Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring","authors":"Xige Zhao, Xiaoyu Zheng, Yijia Wang, Jing Chen, Xiaotong Wang, Xia Peng, Dong Yuan, Ying Liu, Zhiwei Wang, Juan Du","doi":"10.1038/s41368-025-00347-x","DOIUrl":"https://doi.org/10.1038/s41368-025-00347-x","url":null,"abstract":"Periodontal disease is a risk factor for many systemic diseases such as Alzheimer’s disease and adverse pregnancy outcomes. Cleft palate (CP), the most common congenital craniofacial defect, has a multifaceted etiology influenced by complex genetic and environmental risk factors such as maternal bacterial or virus infection. A prior case-control study revealed a surprisingly strong association between maternal periodontal disease and CP in offspring. However, the precise relationship remains unclear. In this study, the relationship between maternal oral pathogen and CP in offspring was studied by sonicated P. gingivalis injected intravenously and orally into pregnant mice. We investigated an obvious increasing CP (12.5%) in sonicated P. gingivalis group which had inhibited osteogenesis in mesenchyme and blocked efferocytosis in epithelium. Then glycolysis and H4K12 lactylation (H4K12la) were detected to elevate in both mouse embryonic palatal mesenchyme (MEPM) cells and macrophages under P. gingivalis exposure which further promoted the transcription of metallopeptidase domain17 (ADAM17), subsequently mediated the shedding of transforming growth factor-beta receptor 1 (TGFBR1) in MEPM cells and mer tyrosine kinase (MerTK) in macrophages and resulted in the suppression of efferocytosis and osteogenesis in palate, eventually caused abnormalities in palate fusion and ossification. The abnormal efferocytosis also led to a predominance of M1 macrophages, which indirectly inhibited palatal osteogenesis via extracellular vesicles. Furthermore, pharmacological ADAM17 inhibition could ameliorate the abnormality of P. gingivalis-induced abnormal palate development. Therefore, our study extends the knowledge of how maternal oral pathogen affects fetal palate development and provides a novel perspective to understand the pathogenesis of CP.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"49 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143608077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expert consensus on the clinical strategies for orthodontic treatment with clear aligners 专家对使用透明矫正器治疗正畸的临床策略的共识

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-13 DOI: 10.1038/s41368-025-00350-2

Yan Wang, Hu Long, Zhihe Zhao, Ding Bai, Xianglong Han, Jun Wang, Bing Fang, Zuolin Jin, Hong He, Yuxin Bai, Weiran Li, Min Hu, Yanheng Zhou, Hong Ai, Yuehua Liu, Yang Cao, Jun Lin, Huang Li, Jie Guo, Wenli Lai

引用次数: 0

Evolution of temporomandibular joint reconstruction: from autologous tissue transplantation to alloplastic joint replacement 颞下颌关节重建的发展：从自体组织移植到同种异体关节置换术

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-10 DOI: 10.1038/s41368-024-00339-3

Hanghang Liu, Liwei Huang, Shibo Liu, Linyi Liu, Bolun Li, Zizhuo Zheng, Yao Liu, Xian Liu, En Luo

{"title":"Evolution of temporomandibular joint reconstruction: from autologous tissue transplantation to alloplastic joint replacement","authors":"Hanghang Liu, Liwei Huang, Shibo Liu, Linyi Liu, Bolun Li, Zizhuo Zheng, Yao Liu, Xian Liu, En Luo","doi":"10.1038/s41368-024-00339-3","DOIUrl":"https://doi.org/10.1038/s41368-024-00339-3","url":null,"abstract":"The reconstruction of the temporomandibular joint presents a multifaceted clinical challenge in the realm of head and neck surgery, underscored by its relatively infrequent occurrence and the lack of comprehensive clinical guidelines. This review aims to elucidate the available approaches for TMJ reconstruction, with a particular emphasis on recent groundbreaking advancements. The current spectrum of TMJ reconstruction integrates diverse surgical techniques, such as costochondral grafting, coronoid process grafting, revascularized fibula transfer, transport distraction osteogenesis, and alloplastic TMJ replacement. Despite the available options, a singular, universally accepted ‘gold standard’ for reconstructive techniques or materials remains elusive in this field. Our review comprehensively summarizes the current available methods of TMJ reconstruction, focusing on both autologous and alloplastic prostheses. It delves into the differences of each surgical technique and outlines the implications of recent technological advances, such as 3D printing, which hold the promise of enhancing surgical precision and patient outcomes. This evolutionary progress aims not only to improve the immediate results of reconstruction but also to ensure the long-term health and functionality of the TMJ, thereby improving the quality of life for patients with end-stage TMJ disorders.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"39 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expert consensus on intentional tooth replantation 专家对有意再植牙齿的共识

IF 14.9 1区医学

International Journal of Oral Science Pub Date : 2025-03-03 DOI: 10.1038/s41368-024-00337-5

Zhengmei Lin, Dingming Huang, Shuheng Huang, Zhi Chen, Qing Yu, Benxiang Hou, Lihong Qiu, Wenxia Chen, Jiyao Li, Xiaoyan Wang, Zhengwei Huang, Jinhua Yu, Jin Zhao, Yihuai Pan, Shuang Pan, Deqin Yang, Weidong Niu, Qi Zhang, Shuli Deng, Jingzhi Ma, Xiuping Meng, Jian Yang, Jiayuan Wu, Lan Zhang, Jin Zhang, Xiaoli Xie, Jinpu Chu, Kehua Que, Xuejun Ge, Xiaojing Huang, Zhe Ma, Lin Yue, Xuedong Zhou, Junqi Ling

{"title":"Expert consensus on intentional tooth replantation","authors":"Zhengmei Lin, Dingming Huang, Shuheng Huang, Zhi Chen, Qing Yu, Benxiang Hou, Lihong Qiu, Wenxia Chen, Jiyao Li, Xiaoyan Wang, Zhengwei Huang, Jinhua Yu, Jin Zhao, Yihuai Pan, Shuang Pan, Deqin Yang, Weidong Niu, Qi Zhang, Shuli Deng, Jingzhi Ma, Xiuping Meng, Jian Yang, Jiayuan Wu, Lan Zhang, Jin Zhang, Xiaoli Xie, Jinpu Chu, Kehua Que, Xuejun Ge, Xiaojing Huang, Zhe Ma, Lin Yue, Xuedong Zhou, Junqi Ling","doi":"10.1038/s41368-024-00337-5","DOIUrl":"https://doi.org/10.1038/s41368-024-00337-5","url":null,"abstract":"Intentional tooth replantation (ITR) is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions. ITR is defined as the deliberate extraction of a tooth; evaluation of the root surface, endodontic manipulation, and repair; and placement of the tooth back into its original socket. Case reports, case series, cohort studies, and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage with root canal treatment or endodontic microsurgery. However, variations in clinical protocols for ITR exist due to the empirical nature of the original protocols and rapid advancements in the field of oral biology and dental materials. This heterogeneity in protocols may cause confusion among dental practitioners; therefore, guidelines and considerations for ITR should be explicated. This expert consensus discusses the biological foundation of ITR, the available clinical protocols and current status of ITR in treating teeth with refractory apical periodontitis or anatomical aberration, and the main complications of this treatment, aiming to refine the clinical management of ITR in accordance with the progress of basic research and clinical studies; the findings suggest that ITR may become a more consistent evidence-based option in dental treatment.","PeriodicalId":14191,"journal":{"name":"International Journal of Oral Science","volume":"45 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0