International Journal of Biomaterials最新文献

{"title":"Determination of Chemical Composition and Antimicrobial Activity of the CO₂ Extract of <i>Eryngium planum</i> L.","authors":"Aliya B Arykbayeva,&nbsp;Gulbaram O Ustenova,&nbsp;Kamalidin O Sharipov,&nbsp;Ulzhan T Beissebayeva,&nbsp;Irina E Kaukhova,&nbsp;Auyes Myrzabayeva,&nbsp;Nadezhda G Gemejiyeva","doi":"10.1155/2023/4702607","DOIUrl":"https://doi.org/10.1155/2023/4702607","url":null,"abstract":"<p><p>The article presents parameters for obtaining a carbon dioxide extract from the subterranean part of <i>Eryngium planum</i> that contains a valuable set of organic substances and has a certain antimicrobial effect. <i>Methods</i>. Raw materials were collected in the Almaty region (Republic of Kazakhstan). The CO₂ extract of <i>Eryngium planum</i> herbs was obtained under subcritical conditions. A gas chromatograph with a mass spectrometric detector was used to determine the compositional breakdown of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilutions and the disk-diffusion method. Three microbial test strains were used: <i>Staphylococcus aureus</i> ATCC 6538-P, <i>Escherichia coli</i> ATCC 8739, and <i>Candida albicans</i> ATCC 10231. <i>Results</i>. To extract biologically active substances from the subterranean part of <i>Eryngium planum</i> L., we have chosen carbon dioxide extraction technology, a technology for processing carbon dioxide (CO₂) raw materials, which allows us to extract various substances in high concentrations. Carbon dioxide extraction technology is an effective and environmentally safe way to isolate various biologically active substances contained in medicinal plant raw materials. In the composition of the CO₂ extract of <i>Eryngium planum</i> L. 43 components were identified, the main of which are <i>α</i>-linolenic acid, 8.30%; myristic acid, 6.40%; caryophyllene, 6.92%; spatulous, 6.62%; and other main identified compounds and their percentage. <i>Conclusions</i>. The study showed that the CO₂ extract of <i>Eryngium planum</i> L. contains biologically active compounds that have a pronounced antimicrobial effect against clinically significant microorganisms, such as <i>Escherichia coli, Staphylococcus aureus,</i> and <i>Candida albicans</i>.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"4702607"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9798463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing.","authors":"Surya Prasad Adhikari,&nbsp;Astha Paudel,&nbsp;Anisha Sharma,&nbsp;Baruna Thapa,&nbsp;Neha Khanal,&nbsp;Nisha Shastri,&nbsp;Sourav Rai,&nbsp;Rameshwar Adhikari","doi":"10.1155/2023/8541621","DOIUrl":"https://doi.org/10.1155/2023/8541621","url":null,"abstract":"<p><p>In this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using <i>Aloe vera</i> leaf at different incubating times were characterized by using X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet-Visible Spectroscopy (UV-Vis spectroscopy). The different characterizations confirmed that the sizes of AgNPs prepared by incubating for 6 hours and 12 hours were 29.1 nm and 35.2 nm, respectively. After that, the different concentrations of the smallest AgNPs were used to dope the DFS scaffold to determine the cell viability. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to correlate the concentration of AgNPs with its bactericidal effect which was seen from 50 <i>μ</i>g/ml. Then, the toxicity with human cells was investigated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay with no significant cell viability from the concentration of 50 <i>μ</i>g/ml to 200 <i>μ</i>g/ml compared to the cocultured and commercial treatments.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"8541621"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9904935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10687081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercially Available Textiles as a Scaffolding Platform for Large-Scale Cell Culture.","authors":"Tarun Agarwal,&nbsp;Sheri-Ann Tan,&nbsp;Shanmuga Sharan Rathnam Vuppaladadium,&nbsp;Tanmayi Sajja,&nbsp;Tapas Kumar Maiti","doi":"10.1155/2023/2227509","DOIUrl":"https://doi.org/10.1155/2023/2227509","url":null,"abstract":"<p><p>The present study outlines the evaluation of textile materials that are currently in the market for cell culture applications. By using normal LaserJet printing techniques, we created the substrates, which were then characterized physicochemically and biologically. In particular, (i) we found that the weave pattern and (ii) the chemical nature of the textiles significantly influenced the behaviour of the cells. Textiles with closely knitted fibers and cell adhesion motifs, exhibited better cell adhesion and proliferation over a period of 7 days. All the substrates supported good viability of cells (>80%). We believe that these aspects make commercially available textiles as a potential candidate for large-scale culture of adherent cells.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"2227509"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9454772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Year Multicenter Randomised Controlled Trial Comparing OT Equator® and Locator Attachments to Retain an Early Loaded Implant Overdenture on Two Implants.","authors":"Marco Tallarico,&nbsp;Luca Fiorillo,&nbsp;Marco Montanari,&nbsp;Roberto Scrascia,&nbsp;Corina Marilena Cristache,&nbsp;Emiliano Ferrari,&nbsp;Alessio Casucci,&nbsp;Erta Xhanari,&nbsp;Saturnino Marco Lupi,&nbsp;Irene Ieria,&nbsp;Edoardo Baldoni,&nbsp;Ruggero Rodriguez Y Baena,&nbsp;Gabriele Cervino","doi":"10.1155/2023/2745262","DOIUrl":"https://doi.org/10.1155/2023/2745262","url":null,"abstract":"<p><p>This investigation aimed to compare the effectiveness of the OT Equator® (Rhein, Bologna, Italy) and the Locator attachment systems used to retain early loaded implant-retained overdentures. This study was designed as a multicenter randomised controlled trial of parallel groups. After implant placement, the patients were randomised to receive OT Equator® attachments in the test group or Locator attachments in the control group. The outcome measures were implant and prosthetic success and survival rates, any biological and technical complication, marginal bone loss, patients' satisfaction, and periodontal parameters. Overall, 42 patients were consecutively enrolled and treated. One implant was lost in the control group, while no implants were lost in the test group. No prostheses failed in both groups. Only a few complications were experienced in both groups. The main was represented by loss of retention of the attachments (retentive caps). The OT Equator® attachment showed statistically lower periodontal parameters. In conclusion, both attachment systems were suitable for overdenture implant retention.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"2745262"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Polyethylene Glycol-Modified Chitosan Scaffolds on the Proliferation and Differentiation of Osteoblasts.","authors":"Wei-Bor Tsai,&nbsp;Ibrahim Nasser Ahmed","doi":"10.1155/2023/4864492","DOIUrl":"https://doi.org/10.1155/2023/4864492","url":null,"abstract":"<p><p>The objective of this study was to investigate the influence of polyethylene glycol (PEG) incorporated chitosan scaffolds on osteoblasts proliferation and differentiation. The chitosan polymer was initially modified by the predetermined concentration of the photoreactive azido group for UV-crosslinking and with RGD peptides (N-acetyl-GRGDSPGYG-amide). The PEG was mixed at different ratios (0, 10, and 20 wt%) with modified chitosan in 96-well tissue culture polystyrene plates to prepare CHI-100, CHI-90, and CHI-80 scaffolds. PEG-containing scaffolds exhibited bigger pore size and higher water content compared to unmodified chitosan scaffolds. After 10 days of incubation, the cell number of CHI-90 (1.1 × 106 cells/scaffold) surpasses that of CHI-100 (9.2 × 105 cells/scaffold) and the cell number of CHI-80 (7.6 × 105 cells/scaffold) were significantly lower. The ALP activity of CHI-90 was the highest on the fifth day indicating the favored osteoblasts' early-stage differentiation. Moreover, after 14 days of osteogenic culture, calcium deposition in the CHI-90 scaffolds (2.7 <i>μ</i>mol Ca/scaffold) was significantly higher than the control (2.2 <i>μ</i>mol Ca/scaffold) whereas on CHI-80 was 1.9 <i>μ</i>mol/scaffold. The results demonstrate that PEG-incorporated chitosan scaffolds favored osteoblasts proliferation and differentiation; however, mixing relatively excess PEG (≥20% wt.) had a negative impact on osteoblasts proliferation and differentiation.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"4864492"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10519052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium Coated with Graphene and Niobium Pentoxide for Biomaterial Applications.","authors":"Hazel Paloma Reis Corado, Francielly Moura de Souza Soraes, Dyanni Manhães Barbosa, Andreza Menezes Lima, Carlos Nelson Elias","doi":"10.1155/2022/2786101","DOIUrl":"10.1155/2022/2786101","url":null,"abstract":"<p><p>Graphene and niobium oxide are used in biomaterial coatings. In this work, commercially pure titanium (cp Ti) was coated with graphene oxide (GO), niobium pentoxide (Nb₂O₅), and a mixture of both materials (NbGO) by the electrochemical deposition method. The surface morphology, roughness, wettability, and degradation of coated and uncoated samples were analyzed by scanning electron microscopy, interferometry, and contact angle. The results showed that the specimens coated with NbGO (cp Ti-NbGO) showed the highest surface roughness (Ra = 0.64 <i>μ</i>m) and were hydrophobic. The contact (<i>θ</i>) angle between water and the surface of uncoated specimens (cp Ti), coated with GO (cp Ti-GO), coated with a mixture with GO and Nb₂O₅) (cp Ti-NbGO), and coated with Nb₂O₅ were 50.74°, 44.35°, 55.86°, and 100.35°, respectively. The electrochemical corrosion tests showed that coating with graphene oxide increased the corrosion resistance and coating with Nb₂O₅ decreased the corrosion resistance. The negative effect of the effect of Nb₂O₅ coating in corrosion resistance compensated for the release of Nb₂O₅, which helps osseointegration, increasing cell viability, and proliferation of osteoblasts. The NbGO coating may be a good way to combine the bactericidal effect of graphene oxide with the osseointegration effect of Nb₂O₅.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"2786101"},"PeriodicalIF":3.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10337284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in the Use of Hydrogels for Regenerative Medicine: Properties and Biomedical Applications.","authors":"Andrea Revete,&nbsp;Andrea Aparicio,&nbsp;Bruno A Cisterna,&nbsp;Javier Revete,&nbsp;Luis Luis,&nbsp;Ernesto Ibarra,&nbsp;Edwin A Segura González,&nbsp;Jay Molino,&nbsp;Diego Reginensi","doi":"10.1155/2022/3606765","DOIUrl":"https://doi.org/10.1155/2022/3606765","url":null,"abstract":"<p><p>Due to their particular water absorption capacity, hydrogels are the most widely used scaffolds in biomedical studies to regenerate damaged tissue. Hydrogels can be used in tissue engineering to design scaffolds for three-dimensional cell culture, providing a novel alternative to the traditional two-dimensional cell culture as hydrogels have a three-dimensional biomimetic structure. This material property is crucial in regenerative medicine, especially for the nervous system, since it is a highly complex and delicate structure. Hydrogels can move quickly within the human body without physically disturbing the environment and possess essential biocompatible properties, as well as the ability to form a mimetic scaffold <i>in situ</i>. Therefore, hydrogels are perfect candidates for biomedical applications. Hydrogels represent a potential alternative to regenerating tissue lost after removing a brain tumor and/or brain injuries. This reason presents them as an exciting alternative to highly complex human physiological problems, such as injuries to the central nervous system and neurodegenerative disease.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":"3606765"},"PeriodicalIF":3.1,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40469591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Oxide Nanoparticles as Diagnostic Tool for Cancer Cells.","authors":"Sumayah Ibraheem,&nbsp;Afraa Ali Kadhim,&nbsp;Kadhim Ali Kadhim,&nbsp;Ihssan A Kadhim,&nbsp;Majid Jabir","doi":"10.1155/2022/2807644","DOIUrl":"https://doi.org/10.1155/2022/2807644","url":null,"abstract":"<p><p>ZnO nanoparticles have various characteristics that make them attractive to be used in many medical applications like a cancer diagnosis. It can be used as a nanoprobe for targeting different types of cancer cells in vitro as a cancer cell recognition system. The present study aims to investigate the permeability of ZnO NPs through both normal and cancerous cell lines in humans. In vitro experiments for ZnO NPs inside the environment of living cells have been described, which would contribute to the visualization of nanoparticles as cancer diagnostic and scanning techniques. MCF7, AMJ13, and RD cancer cells, and also the normal breast cell line HBL, were used in in vitro imaging experiments. The findings revealed that ZnO NPs specifically incorporated within tumor cells while accumulating less inside normal cells. Our findings show that ZnO NPs may be identified inside cancer cells after 1 h of exposure and can endure up to 3 h, providing them appropriate for tumor cell imaging. The findings showed that ZnO NPs might be employed as an alternate fluorophore for diagnostic imaging in the early identification of solid cancers. Therefore, here we studied in vitro applications of ZnO NPs and their beneficial use as a diagnostic tool for cancer cell lines rather than normal cells. Taken together, ZnO NPs can be used as good targeting NPs for the development of imaging agents for early diagnosis of cancers.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":"2807644"},"PeriodicalIF":3.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9646305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40469588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Color Matching of Universal Shade Resin-Based Composite with Natural Teeth and Its Stability before and after In-Office Bleaching.","authors":"AlHanouf AlHabdan,&nbsp;Ahoud AlShamrani,&nbsp;Randa AlHumaidan,&nbsp;AlJohrah AlFehaid,&nbsp;Sara Eisa","doi":"10.1155/2022/8420890","DOIUrl":"https://doi.org/10.1155/2022/8420890","url":null,"abstract":"<p><strong>Objectives: </strong>Esthetics is an essential issue for the long-term success of composite resin restoration. Therefore, this study aimed to view the esthetics of universal shade composite resin restorations and to assess its color matching before and after bleaching.</p><p><strong>Materials and methods: </strong>Overall, 40 freshly extracted premolars were mounted in an acrylic resin mold, and Class V cavities were then prepared and restored by OMNICHROMA composite (Tokuyama Dental, Tokyo, Japan) and polished with 2-step polishing system. Baseline color analysis was performed using VITA Easyshade V digital spectrophotometer, and another color analysis was carried out 24 hours after storage in distilled water. In-office bleaching was carried out, and color measurements were taken after bleaching and 2 weeks postbleaching. The data were statistically analyzed using SPSS 26.0 Windows version statistical software. Changes were considered statistically significant at <i>P</i> = 0.05.</p><p><strong>Results: </strong>ΔE value of OMNICHROMA restoration before and after restoration was 6.474, 3.529 before and after bleaching, and 3.651 two-weeks postbleaching. In-office bleaching was effective in bleaching the OMNICHROMA specimens as the restoration showed positive ΔL ^<i>∗</i> values, which indicated that the specimens were lighter in color after bleaching; however, the bleaching effect was not maintained after 2 weeks.</p><p><strong>Conclusion: </strong>OMNICHROMA universal shade composite resin restoration showed different color matching values with the adjacent enamel of class Vs. The material appeared lighter in shade postbleaching, and the color change was not maintained 2 weeks postbleaching.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":"8420890"},"PeriodicalIF":3.1,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40670499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study of Biotin and Hydroxyapatite on Biological Properties of Composite Coating.","authors":"Qahtan A Hamad,&nbsp;Fatimah J Al-Hasani,&nbsp;Noor K Faheed","doi":"10.1155/2022/8802111","DOIUrl":"https://doi.org/10.1155/2022/8802111","url":null,"abstract":"<p><p>The ultimate goal of using biomaterials is to improve human health by restoring the function of natural living tissues and organs in the body. The present work aims to modify the composite coating layer properties by using two different types of bioactive reinforcing materials (biotin and hydroxyapatite) particles in different percentages (5% and 10%). Coatings were applied onto commercially pure Ti, SS 316 L, and SS 304 substrates by the dip-coating method. Characterization of samples includes microstructure observation by field emission scanning electron microscopy (FE-SEM), contact angle measurement (wettability), and MTT. The results show the addition of metallic particles (bioparticles) (hydroxyapatite particles, biotin) at 5 Vol. % improved the whole properties of composite materials. Using different particles' scale size aids to enhance the combinations in the alginate matrix producing a dual effect on composite film properties. In addition, the inclusion of metallic particles has to increase the wettability by reducing the contact angle. At the same time, MTT graphs revealed that after 3 days of exposure in MG-63 cells, 316 L SS alloys' surface following pack adhesion became more active.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":"8802111"},"PeriodicalIF":3.1,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33490512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}