International Journal of Biomaterials最新文献

{"title":"The Impact of Polyethylene Glycol-Modified Chitosan Scaffolds on the Proliferation and Differentiation of Osteoblasts.","authors":"Wei-Bor Tsai,&nbsp;Ibrahim Nasser Ahmed","doi":"10.1155/2023/4864492","DOIUrl":"https://doi.org/10.1155/2023/4864492","url":null,"abstract":"<p><p>The objective of this study was to investigate the influence of polyethylene glycol (PEG) incorporated chitosan scaffolds on osteoblasts proliferation and differentiation. The chitosan polymer was initially modified by the predetermined concentration of the photoreactive azido group for UV-crosslinking and with RGD peptides (N-acetyl-GRGDSPGYG-amide). The PEG was mixed at different ratios (0, 10, and 20 wt%) with modified chitosan in 96-well tissue culture polystyrene plates to prepare CHI-100, CHI-90, and CHI-80 scaffolds. PEG-containing scaffolds exhibited bigger pore size and higher water content compared to unmodified chitosan scaffolds. After 10 days of incubation, the cell number of CHI-90 (1.1 × 106 cells/scaffold) surpasses that of CHI-100 (9.2 × 105 cells/scaffold) and the cell number of CHI-80 (7.6 × 105 cells/scaffold) were significantly lower. The ALP activity of CHI-90 was the highest on the fifth day indicating the favored osteoblasts' early-stage differentiation. Moreover, after 14 days of osteogenic culture, calcium deposition in the CHI-90 scaffolds (2.7 <i>μ</i>mol Ca/scaffold) was significantly higher than the control (2.2 <i>μ</i>mol Ca/scaffold) whereas on CHI-80 was 1.9 <i>μ</i>mol/scaffold. The results demonstrate that PEG-incorporated chitosan scaffolds favored osteoblasts proliferation and differentiation; however, mixing relatively excess PEG (≥20% wt.) had a negative impact on osteoblasts proliferation and differentiation.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"4864492"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10519052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium Coated with Graphene and Niobium Pentoxide for Biomaterial Applications.","authors":"Hazel Paloma Reis Corado, Francielly Moura de Souza Soraes, Dyanni Manhães Barbosa, Andreza Menezes Lima, Carlos Nelson Elias","doi":"10.1155/2022/2786101","DOIUrl":"10.1155/2022/2786101","url":null,"abstract":"<p><p>Graphene and niobium oxide are used in biomaterial coatings. In this work, commercially pure titanium (cp Ti) was coated with graphene oxide (GO), niobium pentoxide (Nb₂O₅), and a mixture of both materials (NbGO) by the electrochemical deposition method. The surface morphology, roughness, wettability, and degradation of coated and uncoated samples were analyzed by scanning electron microscopy, interferometry, and contact angle. The results showed that the specimens coated with NbGO (cp Ti-NbGO) showed the highest surface roughness (Ra = 0.64 <i>μ</i>m) and were hydrophobic. The contact (<i>θ</i>) angle between water and the surface of uncoated specimens (cp Ti), coated with GO (cp Ti-GO), coated with a mixture with GO and Nb₂O₅) (cp Ti-NbGO), and coated with Nb₂O₅ were 50.74°, 44.35°, 55.86°, and 100.35°, respectively. The electrochemical corrosion tests showed that coating with graphene oxide increased the corrosion resistance and coating with Nb₂O₅ decreased the corrosion resistance. The negative effect of the effect of Nb₂O₅ coating in corrosion resistance compensated for the release of Nb₂O₅, which helps osseointegration, increasing cell viability, and proliferation of osteoblasts. The NbGO coating may be a good way to combine the bactericidal effect of graphene oxide with the osseointegration effect of Nb₂O₅.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"2786101"},"PeriodicalIF":3.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10337284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical Properties and Color Stainability by Coffee and Red Wine of Opaque and High Translucency Zirconia Dental Ceramics after Hydrothermal Degradation","authors":"Ana Lúcia Nascimento Oliveira, C. Elias, Heraldo Elias Salomão Dos Santos, Claudinei dos Santos, R. de Biasi","doi":"10.1155/2022/1571729","DOIUrl":"https://doi.org/10.1155/2022/1571729","url":null,"abstract":"The objective was to evaluate the bending strength, phase transformation, roughness, and color stainability by coffee and red wine of opaque and high translucency yttria-stabilized zirconia before and after hydrothermal degradation in saline solution or oral mouthwash. Presintered zirconia blocks with medium opacity (ZrO2-3 mol. % of Y2O3) designed as ZrOp and high translucency zirconia (5.2 mol. % of Y2O3) designed as ZrTrans were used. Specimens (n = 80/group) were cut and sintered at 1500°C for 2 h. The specimens were hydrothermally degraded in an autoclave (134°C–1.8 kg/cm2) for 20 h in saline solution (0.5 g/L) and oral mouthwash solution (0.02% sodium fluoride, without alcohol and with 21.6% alcohol). After hydrothermal degradation, the samples were immersed in containers with coffee or red wine for 14 days to determine their color stainability. The results showed that the ZrOp had a higher bending strength than the ZrTrans before and after hydrothermal aging. In ZrOp and ZrTrans, the roughness increased after hydrothermal degradation. ZrOp samples had a higher Ra roughness than the ZrTrans samples. Roughness did not change after immersion in coffee or red wine. The X-ray diffraction (XRD) results showed that ZrOp samples underwent a tetragonal to monoclinic phase transformation, while ZrTrans samples were unchanged. Both ZrOp and ZrTrans samples changed color after immersion in coffee and red wine.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43886841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and Biochemical Comparative Study of Copper Oxide Nanoparticles and Copper Sulphate Toxicity in Male Albino Mice Reproductive System","authors":"Manal M. S. AL-Musawi, Hanady S Al-Shmgani, G. Al-Bairuty","doi":"10.1155/2022/4877637","DOIUrl":"https://doi.org/10.1155/2022/4877637","url":null,"abstract":"Copper (Cu) is an essential trace element for the efficient functioning of living organisms. Cu can enter the body in different ways, and when it surpasses the range of biological tolerance, it can have negative consequences. The use of different nanoparticles, especially metal oxide nanoparticles, is increasingly being expanded in the fields of industry and biomedical materials. However, the impact of these nanoparticles on human health is still not completely elucidated. This comparative study was conducted to evaluate the impacts of copper oxide nanoparticles (CuO NPs) and copper sulphate (CuSO4 0.5 (H2O)) on infertility and reproductive function in male albino mice BALB/c. Body weight, the weight of male reproductive organs, malondialdehyde (MDA) level, caspase-3 level, and the presence of Ki67 and CD68, as detected using the amino-histochemistry technique, were investigated. Animals were treated with 25 and 35 mg/kg of CuO NPs and CuSO4 0.5 (H2O) by oral gavage for 14 days. The control group was given distilled water by oral gavage. Body weight significantly decreased at the end of experiments in both treated groups in a concentration- and time-dependent manner compared with the control group. Weights of testes and epididymis (head and tail), as well as the weight of the seminal vesicle, showed a significant decrease compared with the control. However, the average weights of the seminal vesicle and prostate significantly increased. Caspase-3 and MDA levels increased in the CuO NP and CuSO4 0.5 (H2O) groups compared with the control group, and there was a significant difference between the two concentrations used. Immunohistochemical results detected a significant decrease in Ki67 protein in the treatment groups compared with the control. However, increase in CD68 protein was found in groups treated with CuO NPs and CuSO4 0.5 (H2O) compared with the control group. Overall, this in vivo comparative study of CuO NPs and CuSO4 0.5 (H2O) showed that oral intake of copper NPs at 25 and 23 mg/kg was safer to the mice reproductive system than CuSO4 0.5 (H2O) at the same dose. CuSO4 0.5 (H2O) significantly influenced the histopathological and toxicological alteration responses.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42545806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Biomechanical and Chemical Properties of Gamma-Irradiated Polycaprolactone Microfilaments for Musculoskeletal Tissue Engineering Applications","authors":"Laura Rojas-Rojas, Andrea Ulloa-Fernández, Silvia Castro-Piedra, Walter Vargas-Segura, T. Guillén-Girón","doi":"10.1155/2022/5266349","DOIUrl":"https://doi.org/10.1155/2022/5266349","url":null,"abstract":"An appropriate and reliable sterilization technique is crucial for tissue engineering scaffolds. Skeletal muscle scaffolds are often fabricated using microfilaments of a wide variety of polymers. One method for sterilization is 25 kGy of gamma irradiation. In addition, sterilization through irradiation should administer a dose within a specific range. Radiation directly affects the chemical and mechanical properties of scaffolds. The accuracy and effects of irradiation are often not considered during sterilization procedures; however, these are important since they provide insight on whether the sterilization procedure is reliable and reproducible. This study focused on the chemical and mechanical characterization of 25 kGy gamma-irradiated scaffold. The accuracy and uncertainty of the irradiation procedure were also obtained. X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses were performed to determine whether the crystallinity of the polymer changed after irradiation and whether gamma rays influenced its thermal properties. The tensile parameters of the microfilaments were analyzed by comparing irradiated and nonirradiated scaffolds to determine whether gamma radiation changed their elastic behavior. Dose distribution and uncertainty were recorded with several dosimeters. The results showed that the irradiation process slightly affected the mechanical parameters of the scaffold; however, it did not modify its crystallinity or thermal properties. The irradiation was uniform, since the measured uncertainty was low. The scaffold was pathogen-free after 7 days; this meant sterilization was achieved. These results indicated that gamma-sterilized scaffolds were a promising material for use as a skeletal muscle analog material for tissue-engineering applications because they can be sterilized with gamma rays without changing their chemical structure and mechanical properties. This study provided the dose distribution measurement and uncertainty calculations for the sterilization procedure.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42802188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Effect of Incorporation of ZrO2, TiO2, and SiO2 Nanoparticles on the Strength and Surface Properties of PMMA Denture Base Material: An In Vitro Study","authors":"Emad Azmy, M. R. Al-kholy, Ahmad M. Al-Thobity, M. Gad, M. A. Helal","doi":"10.1155/2022/5856545","DOIUrl":"https://doi.org/10.1155/2022/5856545","url":null,"abstract":"Objective This study aimed to investigate the effects of nanoparticles (zirconium dioxide (ZrO2), titanium dioxide (TiO2), and silicon dioxide (SiO2)) on the flexural strength, impact strength, hardness, and wear resistance of the acrylic resin denture base material. Materials and Methods Acrylic resin specimens were fabricated in dimensions according to American Dental Association (ADA) specifications per test. Specimens were divided according to nanofiller into four groups; unmodified as control, ZrO2 (Z), TiO2, (T), and SiO2 (S) groups. Each one was subdivided into two subgroups according to nanoparticle concentrations; 3% and 7% (Z3, Z7, T3, T7, S3, and S7). A 3-point bending test, Charpy impact test, and Vickers hardness test were used for flexural strength, impact strength, and hardness measurements, respectively. Wear resistance was measured by the differences in surface roughness of tested specimens before and after the wear test. A scanning electron microscope was used to assess nanoparticle specifications and distributions and for fracture surfaces analysis. ANOVA, Bonferroni's post hoc test, and the Kruskal–Wallis test were applied for data analysis (α = 0.05). Results Regarding the flexural and impact strength, there was a statistically remarkable increase for all tested groups compared with the control group, except for the T7 and S7 groups (P value <0.001, effect size = 0.893) and (P value <0.001, effect size = 0.759), respectively. There was a statistically significant improvement in the hardness of all tested groups compared with the control group (P value <0.001, effect size = 0.67) except T3 and S3. Regarding wear, a statistically significant enhancement was noticed in the wear resistance of all tested groups (P value <0.001, effect size = 0.685), except for the T7 and S7 groups. Conclusion The flexural strength, impact strength, and wear resistance improved with both concentrations of ZrO2 and low TiO2 and SiO2 concentrations. The hardness increased with both concentrations of ZrO2 and high TiO2 and SiO2 concentrations.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46601263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of CO2 Extract of Portulaca oleracea for Antioxidant Activity from Raw Material Cultivated in Kazakhstan","authors":"Meruyert I Tleubayeva, R. Abdullabekova, U. Datkhayev, M. Ishmuratova, M. Alimzhanova, K. Kozhanova, Aida M. Seitaliyeva, K. Zhakipbekov, Zhanar Iskakova, E. Serikbayeva, E. Flisyuk","doi":"10.1155/2022/6478977","DOIUrl":"https://doi.org/10.1155/2022/6478977","url":null,"abstract":"Medicinal plants remain as an important resource in the fight against many diseases, especially in developing countries. Antioxidants are substances capable of delaying, retarding, and preventing the oxidation of lipids or substances that delay or prevent free radical reactions during lipid oxidation. Natural antioxidants such as ascorbic acid, tocopherol, phenolic compounds, and flavonoids are a safe alternative to chemical antioxidants. In present work, results of antioxidant activity of raw materials from the cultivated plant Portulaca oleracea are presented. The extraction time was optimized to 780 minutes; the yield of extractive substances was 1.25% in the production of CO2 extract under subcritical conditions. For the first time, the antioxidant activity of Portulaca oleracea CO2 extract was determined by the amperometric method. Gas chromatography-mass spectrometry (GC-MS) chemical analysis of Portulaca oleracea CO2 extract dissolved in hexane revealed 37 components, including a complex mixture of aldehydes, alkanes, alkenes, esters, diterpenes, steroids, vitamin E, and carbohydrates. The investigation results showed that the Portulaca oleracea CO2 extract was promising for pharmaceutical, cosmetic, and food industries and had great potential for the prevention and treatment of diseases caused by oxidative stress.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41533629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Novel Antimicrobial Dental Composite Resin with Nano Cerium Oxide Fillers","authors":"E. Varghese, Dhanasekaran Sihivahanan, K. Venkatesh","doi":"10.1155/2022/3912290","DOIUrl":"https://doi.org/10.1155/2022/3912290","url":null,"abstract":"Objectives To assess the antibacterial efficacy of experimental dental composite resin with cerium oxide nanoparticles as fillers. Methods The cerium oxide nanoparticles were prepared by the coprecipitation procedure. Synthesized 3wt% CeO2 nanoparticles were added to the composite resin as antibacterial filler. Experimental composite resin was manually prepared by adding ingredients. The resin matrix consisted of two mixed monomers, bisphenol A-glycidyl methacrylate and triethylene glycol dimethacrylate, diketone as the photo initiator, and N, N-dimethylaminoethyl methacrylate as a coinitiator. The antibacterial efficacy against Streptococcus mutans, Streptococcus mitis, Streptococcus aureus, and Lactobacillus spp. bacterial strains was tested using the microdilution method keeping commercially available 3M Filtek Z250 restorative composite as control. Results The experimental dental composite demonstrated 99.503% efficacy against Streptococcus mutans, 99.441% efficacy against Streptococcus mitis, 99.416% efficacy against Streptococcus aureus, and 99.233% efficacy against Lactobacillus spp. Conclusion Integrating cerium oxide nanoparticles as fillers into dental composite resin can be promising in terms of antibacterial activity, provided furthermore study has to be conducted to examine other properties. Clinical Significance. Previous studies attempted adding CeO2 nanoparticles into acrylic resins that showed improvement in mechanical properties, but literature is nil on the dental composite resin and cerium oxide nanoparticles. This study demonstrates the development of an experimental antibacterial dental composite resin that can resolve most of the problems related to secondary caries around dental composite restorations.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44692572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of Keratin Wastes Using Keratinolytic Microorganisms to Generate Value-Added Products.","authors":"Muhammed Seid Anbesaw","doi":"10.1155/2022/2048031","DOIUrl":"10.1155/2022/2048031","url":null,"abstract":"<p><p>The management of keratinous wastes generated from different industries is becoming a major concern across the world. In each year, more than a billion tons of keratin waste is released into the environment. Despite some trials that have been performed and utilize this waste into valuable products, still a huge amount of keratin waste from different sources is a less explored biomaterial for making valuable products. This indicates that the huge amount of keratin waste is neither disposed properly nor converted into usable products rather thrown away to the environment that causes environmental pollution. Due to the introduction of this waste associated with different pathogenic organisms into soil and water bodies, human beings and other small and large animals are affected by different diseases. Therefore, there is a need for modern and ecofriendly approaches to dispose and convert this waste into usable products. Hence, the objective of this review is to give a concise overview regarding the degradation of keratin waste by biological approaches using keratinase producing microorganisms. The review also focuses on the practical use of keratinases and the economical importance of bioconverted products of keratinous wastes for different applications. Various researches have been studied about the source, disposal mechanisms, techniques of hydrolysis, potential use, and physical and chemical properties of keratin wastes. However, there is negligible information with regard to the use of keratin wastes as media supplements for the growth of keratinolytic microorganisms and silver retrieval from photographic and used X-ray films. Hence, this review differs from other similar reviews in the literature in that it discusses these neglected concerns.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"2048031"},"PeriodicalIF":3.0,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9599591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Combination of Chitosan Gel and Hydroxyapatite from Crabs Shells (Portunus pelagicus) Waste as Bonegraft on Periodontal Network Regeneration through IL-1 and BMP-2 Analysis","authors":"Asdar Gani, R. Yulianty, Supiaty Supiaty, Machirah Rusdy, Gustivanny Dwipa Asri, Dian Eka Satya, Ayu Rahayu Feblina, H. Achmad","doi":"10.1155/2022/1817236","DOIUrl":"https://doi.org/10.1155/2022/1817236","url":null,"abstract":"Background Periodontitis can be treated by regenerating periodontal tissue using a bone graft. Several natural materials such as chitosan and minerals such as hydroxyapatite can be developed to increase periodontal tissue regeneration. Chitosan has a high potential in healing wounds. Hydroxyapatite has excellent properties such as biocompatibility, osteoconductive, osteoinductive, and osteogenesis, making it an ideal material for soft and hard tissue regeneration. Chitosan and hydroxyapatite can be obtained from the shells of crustaceans, such as crabs shells (Portunus pelagicus). Objective To assess the effectiveness of the combination of chitosan gel and hydroxyapatite powder as a bone graft on periodontal tissue regeneration in experimental animals. Periodontal tissue regeneration was assessed by expressing inflammatory cytokine gene indicators IL-1 and BMP-2. Methods Experimental laboratory research and clinical trials with posttest only control group design. Twenty-seven Wistar rats were divided into three groups. Then the femoral bone defect was made, the positive control group was given placebo gel, the positive control group was given BATAN hydroxyapatite, and the test group was given a combination of chitosan gel and hydroxyapatite crab shells. Wistar rats were sacrificed on days 7, 14, and 21, and the femur bone was then taken for immunohistochemical analysis to determine the levels of IL-1 and BMP-2. The Kolmogorov–Smirnov test, Levene test, and one-way ANOVA analyzed the data. Results On days 7, 14, and 21, the expression levels of IL-1 and BMP2 were significantly different between the three groups. The group added with chitosan gel and crab shell HA showed a faster decrease in IL-1 expression than the control group. BMP-2 expression increased in the test group compared to the control group. Conclusion The combination of chitosan gel and hydroxyapatite inhibited the production of proinflammatory cytokines and increased the production of BMP-2.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45490720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}