International Journal of Biomaterials最新文献

{"title":"The Influence of the Hyaluronic Acid Gel on the Postoperative Sequelae following Surgical Removal of the Impacted Mandibular Third Molar in Comparison with the A-PRF: A Randomized Controlled Trial.","authors":"Thoulfokar Shokor Al-Saadi,&nbsp;Ahmed Fadhel Al-Quisi","doi":"10.1155/2023/1883460","DOIUrl":"https://doi.org/10.1155/2023/1883460","url":null,"abstract":"<p><p>One of the most common procedures in oral surgery is the removal of impacted mandibular third molars, often followed by pain, swelling, alveolitis, and trismus. <i>Purpose</i>. To compare the outcomes of the intrasocket application of 1% hyaluronic acid oral gel (HA) and advanced platelet-rich fibrin (A-PRF) on the expected postoperative complications, pain, swelling, and trismus follow the surgical extraction of the impacted mandibular third molar. <i>Material and Methods</i>. A randomized controlled trial was conducted at the Oral and Maxillofacial Surgery Unit, Dental Teaching Hospital. Healthy patients who required surgical removal of the impacted mandibular third molar were divided randomly into three groups. The extraction site of the group (A) patients remained without the addition of any material, just suturing of the wound with simple interrupted sutures, while in group (B) patients, the extraction site was filled with 1 cc of 1% hyaluronic acid gel (periokin®), and in group C patients, the extraction site was filled with A-PRF. <i>Results</i>. Sixty-six eligible patients participated in this study; both hyaluronic acid gel 1% (periokin®) and advanced platelet-rich fibrin showed a significant reduction in pain, swelling, and trismus on the 1st, third, and seventh postoperative days when compared to the control group, while the comparison between HA and A-PRF showed no significant differences except for the pain on the third postoperative day. There was a significant pain decrease in the A-PRF group than HA group. <i>Conclusion</i>. Intrasocket application of 1% hyaluronic acid gel (periokin®) or advanced platelet-rich fibrin can be an effective primary way to significantly reduce postoperative pain, trismus, and edema compared to the control group following mandibular third molar surgery.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"1883460"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9448788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction and Characterization of Type I Collagen from Parrotfish (<i>Scarus sordidus</i> Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin.","authors":"Abdul Aziz Jaziri,&nbsp;Rossita Shapawi,&nbsp;Ruzaidi Azli Mohd Mokhtar,&nbsp;Wan Norhana Md Noordin,&nbsp;Nurul Huda","doi":"10.1155/2023/7312447","DOIUrl":"https://doi.org/10.1155/2023/7312447","url":null,"abstract":"<p><p>Waste from marine fish processing is an important source of valuable products. Fish collagen is considered a alternative biomaterial due to its excellent properties, and it is widely used for industrial purposes. Thus, this present study aimed to characterize acid and pepsin-soluble collagens from the waste of parrotfish (<i>Scarus sordidus</i> Forsskål, 1775) scales. The yields (<i>p</i> > 0.05) of acid-soluble collagen (ASC-PFS) and pepsin-soluble collagen (PSC-PFS) were 1.17 g/100 g and 1.00 g/100 g, respectively. Both collagen samples were categorized as type I owing to the presence of two alpha chain subunits (<i>α</i>1 and <i>α</i>1) after being confirmed by a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Under the fourier transform infrared (FTIR) test, the triple helical structure of type I collagens from the ASC-PFS and PSC-PFS was maintained. Moreover, the study of UV visible spectra and X-ray diffraction (XRD) showed the similarity of collagens derived from different fish species, and the thermostability (<i>T</i> _max) evaluation of all extracted collagens was in the range of 36.22-37.78°C, and their values were comparable to previous research on the fish scale collagens. The effect of various pH and sodium chloride (NaCl) treatments on solubility exhibited that the ASC-PFS and PSC-PFS were highly soluble in an acidic condition (pH < 5.0) and low concentration of sodium chloride (<30 g/L). Taken together, collagens extracted from parrotfish scale waste can be an alternative source for industries.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"7312447"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10156459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9429410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Chemical Composition and Antimicrobial Activity of the CO₂ Extract of <i>Eryngium planum</i> L.","authors":"Aliya B Arykbayeva,&nbsp;Gulbaram O Ustenova,&nbsp;Kamalidin O Sharipov,&nbsp;Ulzhan T Beissebayeva,&nbsp;Irina E Kaukhova,&nbsp;Auyes Myrzabayeva,&nbsp;Nadezhda G Gemejiyeva","doi":"10.1155/2023/4702607","DOIUrl":"https://doi.org/10.1155/2023/4702607","url":null,"abstract":"<p><p>The article presents parameters for obtaining a carbon dioxide extract from the subterranean part of <i>Eryngium planum</i> that contains a valuable set of organic substances and has a certain antimicrobial effect. <i>Methods</i>. Raw materials were collected in the Almaty region (Republic of Kazakhstan). The CO₂ extract of <i>Eryngium planum</i> herbs was obtained under subcritical conditions. A gas chromatograph with a mass spectrometric detector was used to determine the compositional breakdown of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilutions and the disk-diffusion method. Three microbial test strains were used: <i>Staphylococcus aureus</i> ATCC 6538-P, <i>Escherichia coli</i> ATCC 8739, and <i>Candida albicans</i> ATCC 10231. <i>Results</i>. To extract biologically active substances from the subterranean part of <i>Eryngium planum</i> L., we have chosen carbon dioxide extraction technology, a technology for processing carbon dioxide (CO₂) raw materials, which allows us to extract various substances in high concentrations. Carbon dioxide extraction technology is an effective and environmentally safe way to isolate various biologically active substances contained in medicinal plant raw materials. In the composition of the CO₂ extract of <i>Eryngium planum</i> L. 43 components were identified, the main of which are <i>α</i>-linolenic acid, 8.30%; myristic acid, 6.40%; caryophyllene, 6.92%; spatulous, 6.62%; and other main identified compounds and their percentage. <i>Conclusions</i>. The study showed that the CO₂ extract of <i>Eryngium planum</i> L. contains biologically active compounds that have a pronounced antimicrobial effect against clinically significant microorganisms, such as <i>Escherichia coli, Staphylococcus aureus,</i> and <i>Candida albicans</i>.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"4702607"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9798463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing.","authors":"Surya Prasad Adhikari,&nbsp;Astha Paudel,&nbsp;Anisha Sharma,&nbsp;Baruna Thapa,&nbsp;Neha Khanal,&nbsp;Nisha Shastri,&nbsp;Sourav Rai,&nbsp;Rameshwar Adhikari","doi":"10.1155/2023/8541621","DOIUrl":"https://doi.org/10.1155/2023/8541621","url":null,"abstract":"<p><p>In this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using <i>Aloe vera</i> leaf at different incubating times were characterized by using X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet-Visible Spectroscopy (UV-Vis spectroscopy). The different characterizations confirmed that the sizes of AgNPs prepared by incubating for 6 hours and 12 hours were 29.1 nm and 35.2 nm, respectively. After that, the different concentrations of the smallest AgNPs were used to dope the DFS scaffold to determine the cell viability. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to correlate the concentration of AgNPs with its bactericidal effect which was seen from 50 <i>μ</i>g/ml. Then, the toxicity with human cells was investigated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay with no significant cell viability from the concentration of 50 <i>μ</i>g/ml to 200 <i>μ</i>g/ml compared to the cocultured and commercial treatments.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"8541621"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9904935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10687081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercially Available Textiles as a Scaffolding Platform for Large-Scale Cell Culture.","authors":"Tarun Agarwal,&nbsp;Sheri-Ann Tan,&nbsp;Shanmuga Sharan Rathnam Vuppaladadium,&nbsp;Tanmayi Sajja,&nbsp;Tapas Kumar Maiti","doi":"10.1155/2023/2227509","DOIUrl":"https://doi.org/10.1155/2023/2227509","url":null,"abstract":"<p><p>The present study outlines the evaluation of textile materials that are currently in the market for cell culture applications. By using normal LaserJet printing techniques, we created the substrates, which were then characterized physicochemically and biologically. In particular, (i) we found that the weave pattern and (ii) the chemical nature of the textiles significantly influenced the behaviour of the cells. Textiles with closely knitted fibers and cell adhesion motifs, exhibited better cell adhesion and proliferation over a period of 7 days. All the substrates supported good viability of cells (>80%). We believe that these aspects make commercially available textiles as a potential candidate for large-scale culture of adherent cells.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"2227509"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9454772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Year Multicenter Randomised Controlled Trial Comparing OT Equator® and Locator Attachments to Retain an Early Loaded Implant Overdenture on Two Implants.","authors":"Marco Tallarico,&nbsp;Luca Fiorillo,&nbsp;Marco Montanari,&nbsp;Roberto Scrascia,&nbsp;Corina Marilena Cristache,&nbsp;Emiliano Ferrari,&nbsp;Alessio Casucci,&nbsp;Erta Xhanari,&nbsp;Saturnino Marco Lupi,&nbsp;Irene Ieria,&nbsp;Edoardo Baldoni,&nbsp;Ruggero Rodriguez Y Baena,&nbsp;Gabriele Cervino","doi":"10.1155/2023/2745262","DOIUrl":"https://doi.org/10.1155/2023/2745262","url":null,"abstract":"<p><p>This investigation aimed to compare the effectiveness of the OT Equator® (Rhein, Bologna, Italy) and the Locator attachment systems used to retain early loaded implant-retained overdentures. This study was designed as a multicenter randomised controlled trial of parallel groups. After implant placement, the patients were randomised to receive OT Equator® attachments in the test group or Locator attachments in the control group. The outcome measures were implant and prosthetic success and survival rates, any biological and technical complication, marginal bone loss, patients' satisfaction, and periodontal parameters. Overall, 42 patients were consecutively enrolled and treated. One implant was lost in the control group, while no implants were lost in the test group. No prostheses failed in both groups. Only a few complications were experienced in both groups. The main was represented by loss of retention of the attachments (retentive caps). The OT Equator® attachment showed statistically lower periodontal parameters. In conclusion, both attachment systems were suitable for overdenture implant retention.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"2745262"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Polyethylene Glycol-Modified Chitosan Scaffolds on the Proliferation and Differentiation of Osteoblasts.","authors":"Wei-Bor Tsai,&nbsp;Ibrahim Nasser Ahmed","doi":"10.1155/2023/4864492","DOIUrl":"https://doi.org/10.1155/2023/4864492","url":null,"abstract":"<p><p>The objective of this study was to investigate the influence of polyethylene glycol (PEG) incorporated chitosan scaffolds on osteoblasts proliferation and differentiation. The chitosan polymer was initially modified by the predetermined concentration of the photoreactive azido group for UV-crosslinking and with RGD peptides (N-acetyl-GRGDSPGYG-amide). The PEG was mixed at different ratios (0, 10, and 20 wt%) with modified chitosan in 96-well tissue culture polystyrene plates to prepare CHI-100, CHI-90, and CHI-80 scaffolds. PEG-containing scaffolds exhibited bigger pore size and higher water content compared to unmodified chitosan scaffolds. After 10 days of incubation, the cell number of CHI-90 (1.1 × 106 cells/scaffold) surpasses that of CHI-100 (9.2 × 105 cells/scaffold) and the cell number of CHI-80 (7.6 × 105 cells/scaffold) were significantly lower. The ALP activity of CHI-90 was the highest on the fifth day indicating the favored osteoblasts' early-stage differentiation. Moreover, after 14 days of osteogenic culture, calcium deposition in the CHI-90 scaffolds (2.7 <i>μ</i>mol Ca/scaffold) was significantly higher than the control (2.2 <i>μ</i>mol Ca/scaffold) whereas on CHI-80 was 1.9 <i>μ</i>mol/scaffold. The results demonstrate that PEG-incorporated chitosan scaffolds favored osteoblasts proliferation and differentiation; however, mixing relatively excess PEG (≥20% wt.) had a negative impact on osteoblasts proliferation and differentiation.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2023 ","pages":"4864492"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10519052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium Coated with Graphene and Niobium Pentoxide for Biomaterial Applications.","authors":"Hazel Paloma Reis Corado, Francielly Moura de Souza Soraes, Dyanni Manhães Barbosa, Andreza Menezes Lima, Carlos Nelson Elias","doi":"10.1155/2022/2786101","DOIUrl":"10.1155/2022/2786101","url":null,"abstract":"<p><p>Graphene and niobium oxide are used in biomaterial coatings. In this work, commercially pure titanium (cp Ti) was coated with graphene oxide (GO), niobium pentoxide (Nb₂O₅), and a mixture of both materials (NbGO) by the electrochemical deposition method. The surface morphology, roughness, wettability, and degradation of coated and uncoated samples were analyzed by scanning electron microscopy, interferometry, and contact angle. The results showed that the specimens coated with NbGO (cp Ti-NbGO) showed the highest surface roughness (Ra = 0.64 <i>μ</i>m) and were hydrophobic. The contact (<i>θ</i>) angle between water and the surface of uncoated specimens (cp Ti), coated with GO (cp Ti-GO), coated with a mixture with GO and Nb₂O₅) (cp Ti-NbGO), and coated with Nb₂O₅ were 50.74°, 44.35°, 55.86°, and 100.35°, respectively. The electrochemical corrosion tests showed that coating with graphene oxide increased the corrosion resistance and coating with Nb₂O₅ decreased the corrosion resistance. The negative effect of the effect of Nb₂O₅ coating in corrosion resistance compensated for the release of Nb₂O₅, which helps osseointegration, increasing cell viability, and proliferation of osteoblasts. The NbGO coating may be a good way to combine the bactericidal effect of graphene oxide with the osseointegration effect of Nb₂O₅.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"2786101"},"PeriodicalIF":3.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10337284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical Properties and Color Stainability by Coffee and Red Wine of Opaque and High Translucency Zirconia Dental Ceramics after Hydrothermal Degradation","authors":"Ana Lúcia Nascimento Oliveira, C. Elias, Heraldo Elias Salomão Dos Santos, Claudinei dos Santos, R. de Biasi","doi":"10.1155/2022/1571729","DOIUrl":"https://doi.org/10.1155/2022/1571729","url":null,"abstract":"The objective was to evaluate the bending strength, phase transformation, roughness, and color stainability by coffee and red wine of opaque and high translucency yttria-stabilized zirconia before and after hydrothermal degradation in saline solution or oral mouthwash. Presintered zirconia blocks with medium opacity (ZrO2-3 mol. % of Y2O3) designed as ZrOp and high translucency zirconia (5.2 mol. % of Y2O3) designed as ZrTrans were used. Specimens (n = 80/group) were cut and sintered at 1500°C for 2 h. The specimens were hydrothermally degraded in an autoclave (134°C–1.8 kg/cm2) for 20 h in saline solution (0.5 g/L) and oral mouthwash solution (0.02% sodium fluoride, without alcohol and with 21.6% alcohol). After hydrothermal degradation, the samples were immersed in containers with coffee or red wine for 14 days to determine their color stainability. The results showed that the ZrOp had a higher bending strength than the ZrTrans before and after hydrothermal aging. In ZrOp and ZrTrans, the roughness increased after hydrothermal degradation. ZrOp samples had a higher Ra roughness than the ZrTrans samples. Roughness did not change after immersion in coffee or red wine. The X-ray diffraction (XRD) results showed that ZrOp samples underwent a tetragonal to monoclinic phase transformation, while ZrTrans samples were unchanged. Both ZrOp and ZrTrans samples changed color after immersion in coffee and red wine.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43886841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and Biochemical Comparative Study of Copper Oxide Nanoparticles and Copper Sulphate Toxicity in Male Albino Mice Reproductive System","authors":"Manal M. S. AL-Musawi, Hanady S Al-Shmgani, G. Al-Bairuty","doi":"10.1155/2022/4877637","DOIUrl":"https://doi.org/10.1155/2022/4877637","url":null,"abstract":"Copper (Cu) is an essential trace element for the efficient functioning of living organisms. Cu can enter the body in different ways, and when it surpasses the range of biological tolerance, it can have negative consequences. The use of different nanoparticles, especially metal oxide nanoparticles, is increasingly being expanded in the fields of industry and biomedical materials. However, the impact of these nanoparticles on human health is still not completely elucidated. This comparative study was conducted to evaluate the impacts of copper oxide nanoparticles (CuO NPs) and copper sulphate (CuSO4 0.5 (H2O)) on infertility and reproductive function in male albino mice BALB/c. Body weight, the weight of male reproductive organs, malondialdehyde (MDA) level, caspase-3 level, and the presence of Ki67 and CD68, as detected using the amino-histochemistry technique, were investigated. Animals were treated with 25 and 35 mg/kg of CuO NPs and CuSO4 0.5 (H2O) by oral gavage for 14 days. The control group was given distilled water by oral gavage. Body weight significantly decreased at the end of experiments in both treated groups in a concentration- and time-dependent manner compared with the control group. Weights of testes and epididymis (head and tail), as well as the weight of the seminal vesicle, showed a significant decrease compared with the control. However, the average weights of the seminal vesicle and prostate significantly increased. Caspase-3 and MDA levels increased in the CuO NP and CuSO4 0.5 (H2O) groups compared with the control group, and there was a significant difference between the two concentrations used. Immunohistochemical results detected a significant decrease in Ki67 protein in the treatment groups compared with the control. However, increase in CD68 protein was found in groups treated with CuO NPs and CuSO4 0.5 (H2O) compared with the control group. Overall, this in vivo comparative study of CuO NPs and CuSO4 0.5 (H2O) showed that oral intake of copper NPs at 25 and 23 mg/kg was safer to the mice reproductive system than CuSO4 0.5 (H2O) at the same dose. CuSO4 0.5 (H2O) significantly influenced the histopathological and toxicological alteration responses.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42545806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}