甲醛和姜黄素对Wistar大鼠组织形态学指标、卵泡发育相关基因表达及总抗氧化水平的影响。

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
Zahra Farshad, Abbas Shahedi, Farzaneh Fesahat, Azam Hassanpour, Morteza Anvari
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引用次数: 0

摘要

本实验研究探讨了甲醛(FA)和姜黄素(CUR)对FA暴露大鼠滤泡发育相关基因的组织形态学特征、抗氧化潜能和信使核糖核酸(mRNA)水平的影响。24只Wistar雌性大鼠分为4个研究组,分别腹腔注射FA (10 mg/kg) (N = 6)、FA (10 mg/kg) + CUR (100 mg/kg) (N = 6)、假药(N = 6)和对照组(N = 6),持续14 d。分别用卵巢组织和血清评估卵巢卵泡组织学、相关基因表达、血液因子和抗/氧化电位。与对照组和假药组相比,FA组的klotho明显过表达。相反,与对照组相比,FA和FA + CUR组的种系α因子显著下调。FA组卵巢卵泡数量显著下降,与发育阶段无关。至于FA + CUR组与其他组的比较,在继发卵泡、扁平卵泡和闭锁卵泡的数量上有显著变化。与对照组相比,FA组的血红蛋白、红细胞计数、红细胞压积和平均红细胞血红蛋白浓度显著降低。与对照组相比,FA组谷胱甘肽过氧化物酶活性显著升高。尽管FA对大鼠卵巢卵泡的组织学和分子方面有有害影响,但CUR似乎对这种化学物质的有害影响没有保护作用。然而,在某些情况下,CUR有积极的作用,如减少滤泡破坏和间质水肿。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats.

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats.

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats.

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats.

The present experimental study was undertaken to investigate the effect of formaldehyde (FA) and curcumin (CUR) on histomorphological features, antioxidant potential, and messenger ribonucleic acid (mRNA) levels of genes related to follicular development in FA-exposed rats. 24 Wistar female rats were divided into four study groups and given intraperitoneal injections of FA (10 mg/kg) (N = 6), FA (10 mg/kg) + CUR (100 mg/kg) (N = 6), sham (N = 6), and control (N = 6) for 14 days. Ovarian follicular histology, the related gene expression, blood factors, and anti/oxidation potentials were assessed using ovarian tissue and serum, respectively. The klotho was significantly overexpressed in the FA group compared with controls and shams. Contradictory, the factor in germ line alpha was significantly down-regulated in FA and FA + CUR groups compared to shams and controls. A significant decline was seen in the number of ovarian follicles in the FA group, independent of the developmental stage. Regarding the comparison of the FA + CUR group to other groups, a significant change was seen in the number of secondary, graafian, and atretic follicles. The FA group demonstrated significantly lower hemoglobin, red blood cell count, hematocrit, and mean corpuscular hemoglobin concentration than controls. The activity of glutathione peroxidase increased significantly in the FA group than in the controls. Despite the deleterious effects of FA on histological and molecular aspects of rat ovarian follicles, CUR does not appear to have a protective effect against the hazardous effects of this chemical. However, CUR in some cases has positive effects such as reducing follicular destruction and interstitial edema.

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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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