{"title":"水凝胶透明质酸-壳聚糖-葱提取物的合成与表征。","authors":"Prihartini Widiyanti, Yolanda Citra Ayu Priskawati","doi":"10.1155/2023/5172391","DOIUrl":null,"url":null,"abstract":"<p><p>Peritoneal Adhesion is a severe case that frequently occurs in patients after laparotomy surgery. Adhesions are pathological attachment that usually appears between the omentum, intestine, and abdominal wall. Several barriers are made to prevent adhesions, including liquid barriers such as sodium hyaluronate and carboxymethyl cellulose (CMC) but are fast absorbed-time hydrogel. The solid barrier has weakness of difficulty in covering all parts of the wound surface. The study aims to synthesize degradable hydrogel from N,O-Carboxymethyl Chitosan (NOCC), Aldehyde-Hyaluronic Acid, and the addition of <i>Allium sativum</i> (garlic oil). The best sample with the concentration of A-HA/NOCC 30 : 10 g/ml was obtained. The composite hydrogel of NOCC/AHA/<i>Allium sativum</i> has susceptible antimicrobial properties. <i>In vitro</i> cytotoxicity assay showed that hydrogel is nontoxic. The degradation time is for two weeks. The in vivo evaluation in a mouse model with an abrasion defect side was done to identify the effectiveness of the NOCC/AHA/<i>A. sativum</i> as antiperitoneal adhesion. Seven days after surgery, the observation of adhesion was performed. Based on all assay results, it can be resumed that the NOCC/AHA/<i>A. sativum</i> hydrogel possibly acts as an innovation to prevent postoperative intraperitoneal adhesion.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005870/pdf/","citationCount":"2","resultStr":"{\"title\":\"Synthesis and Characterization of Hydrogel-Based Hyaluronic Acid-Chitosan-<i>Allium sativum</i> Extract for Intraperitoneal Antiadhesion Application.\",\"authors\":\"Prihartini Widiyanti, Yolanda Citra Ayu Priskawati\",\"doi\":\"10.1155/2023/5172391\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Peritoneal Adhesion is a severe case that frequently occurs in patients after laparotomy surgery. Adhesions are pathological attachment that usually appears between the omentum, intestine, and abdominal wall. Several barriers are made to prevent adhesions, including liquid barriers such as sodium hyaluronate and carboxymethyl cellulose (CMC) but are fast absorbed-time hydrogel. The solid barrier has weakness of difficulty in covering all parts of the wound surface. The study aims to synthesize degradable hydrogel from N,O-Carboxymethyl Chitosan (NOCC), Aldehyde-Hyaluronic Acid, and the addition of <i>Allium sativum</i> (garlic oil). The best sample with the concentration of A-HA/NOCC 30 : 10 g/ml was obtained. The composite hydrogel of NOCC/AHA/<i>Allium sativum</i> has susceptible antimicrobial properties. <i>In vitro</i> cytotoxicity assay showed that hydrogel is nontoxic. The degradation time is for two weeks. The in vivo evaluation in a mouse model with an abrasion defect side was done to identify the effectiveness of the NOCC/AHA/<i>A. sativum</i> as antiperitoneal adhesion. Seven days after surgery, the observation of adhesion was performed. Based on all assay results, it can be resumed that the NOCC/AHA/<i>A. sativum</i> hydrogel possibly acts as an innovation to prevent postoperative intraperitoneal adhesion.</p>\",\"PeriodicalId\":13704,\"journal\":{\"name\":\"International Journal of Biomaterials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005870/pdf/\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biomaterials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2023/5172391\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2023/5172391","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 2
摘要
腹膜粘连是剖腹手术后常见的严重并发症。粘连是通常出现在大网膜、肠和腹壁之间的病理性附着。有几种屏障可以防止粘连,包括液体屏障,如透明质酸钠和羧甲基纤维素(CMC),但它们是快速吸收的水凝胶。固体屏障的缺点是难以覆盖创面的所有部位。以N, o -羧甲基壳聚糖(NOCC)和醛透明质酸为原料,添加大蒜油合成可降解水凝胶。最佳样品为A-HA/NOCC浓度为30:10 g/ml。NOCC/AHA/Allium sativum复合水凝胶具有敏感的抗菌性能。体外细胞毒性试验表明,水凝胶无毒。降解时间为两周。在具有磨损缺陷侧的小鼠模型中进行了体内评价,以确定NOCC/AHA/ a的有效性。Sativum作为抗腹膜粘连。术后7 d观察粘连情况。根据所有检测结果,可以恢复NOCC/AHA/A。Sativum水凝胶可能是防止术后腹腔粘连的一种创新。
Synthesis and Characterization of Hydrogel-Based Hyaluronic Acid-Chitosan-Allium sativum Extract for Intraperitoneal Antiadhesion Application.
Peritoneal Adhesion is a severe case that frequently occurs in patients after laparotomy surgery. Adhesions are pathological attachment that usually appears between the omentum, intestine, and abdominal wall. Several barriers are made to prevent adhesions, including liquid barriers such as sodium hyaluronate and carboxymethyl cellulose (CMC) but are fast absorbed-time hydrogel. The solid barrier has weakness of difficulty in covering all parts of the wound surface. The study aims to synthesize degradable hydrogel from N,O-Carboxymethyl Chitosan (NOCC), Aldehyde-Hyaluronic Acid, and the addition of Allium sativum (garlic oil). The best sample with the concentration of A-HA/NOCC 30 : 10 g/ml was obtained. The composite hydrogel of NOCC/AHA/Allium sativum has susceptible antimicrobial properties. In vitro cytotoxicity assay showed that hydrogel is nontoxic. The degradation time is for two weeks. The in vivo evaluation in a mouse model with an abrasion defect side was done to identify the effectiveness of the NOCC/AHA/A. sativum as antiperitoneal adhesion. Seven days after surgery, the observation of adhesion was performed. Based on all assay results, it can be resumed that the NOCC/AHA/A. sativum hydrogel possibly acts as an innovation to prevent postoperative intraperitoneal adhesion.