Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing.

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
Surya Prasad Adhikari, Astha Paudel, Anisha Sharma, Baruna Thapa, Neha Khanal, Nisha Shastri, Sourav Rai, Rameshwar Adhikari
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引用次数: 1

Abstract

In this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using Aloe vera leaf at different incubating times were characterized by using X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet-Visible Spectroscopy (UV-Vis spectroscopy). The different characterizations confirmed that the sizes of AgNPs prepared by incubating for 6 hours and 12 hours were 29.1 nm and 35.2 nm, respectively. After that, the different concentrations of the smallest AgNPs were used to dope the DFS scaffold to determine the cell viability. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to correlate the concentration of AgNPs with its bactericidal effect which was seen from 50 μg/ml. Then, the toxicity with human cells was investigated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay with no significant cell viability from the concentration of 50 μg/ml to 200 μg/ml compared to the cocultured and commercial treatments.

Abstract Image

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生物合成纳米银修饰脱细胞鱼皮支架加速烧伤创面愈合的研究。
本研究制备了纳米银修饰的脱细胞鱼皮(DFS)支架,用于促进烧伤创面愈合。利用不同孵育时间的芦荟叶为原料,采用绿色易溶法合成银纳米颗粒(AgNPs),采用x射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)和紫外可见光谱(UV-Vis)对其进行了表征。不同的表征结果证实,经培养6小时和12小时制备的AgNPs尺寸分别为29.1 nm和35.2 nm。然后,用不同浓度的最小AgNPs涂布DFS支架,测定细胞活力。此外,琼脂孔扩散法筛选抗菌活性。用最小抑菌浓度(MIC)和最小杀菌浓度(MBC)测定AgNPs的浓度与抑菌效果的关系,抑菌效果在50 μg/ml左右。然后,用3-(4,5 -二甲基噻唑-2-基)- 2,5 -二苯基溴化四唑(MTT)法研究其对人细胞的毒性,在浓度为50 μg/ml至200 μg/ml时,与共培养和商业处理相比,细胞活力不显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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