Determination of Chemical Composition and Antimicrobial Activity of the CO2 Extract of Eryngium planum L.

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
Aliya B Arykbayeva, Gulbaram O Ustenova, Kamalidin O Sharipov, Ulzhan T Beissebayeva, Irina E Kaukhova, Auyes Myrzabayeva, Nadezhda G Gemejiyeva
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Abstract

The article presents parameters for obtaining a carbon dioxide extract from the subterranean part of Eryngium planum that contains a valuable set of organic substances and has a certain antimicrobial effect. Methods. Raw materials were collected in the Almaty region (Republic of Kazakhstan). The CO2 extract of Eryngium planum herbs was obtained under subcritical conditions. A gas chromatograph with a mass spectrometric detector was used to determine the compositional breakdown of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilutions and the disk-diffusion method. Three microbial test strains were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231. Results. To extract biologically active substances from the subterranean part of Eryngium planum L., we have chosen carbon dioxide extraction technology, a technology for processing carbon dioxide (CO2) raw materials, which allows us to extract various substances in high concentrations. Carbon dioxide extraction technology is an effective and environmentally safe way to isolate various biologically active substances contained in medicinal plant raw materials. In the composition of the CO2 extract of Eryngium planum L. 43 components were identified, the main of which are α-linolenic acid, 8.30%; myristic acid, 6.40%; caryophyllene, 6.92%; spatulous, 6.62%; and other main identified compounds and their percentage. Conclusions. The study showed that the CO2 extract of Eryngium planum L. contains biologically active compounds that have a pronounced antimicrobial effect against clinically significant microorganisms, such as Escherichia coli, Staphylococcus aureus, and Candida albicans.

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平胸羊奶CO2提取物的化学成分及抑菌活性测定。
本文介绍了从平原鹰隼地下部分提取二氧化碳提取物的工艺参数,该提取物含有一组有价值的有机物,并具有一定的抗菌作用。方法。原材料在阿拉木图地区(哈萨克斯坦共和国)收集。在亚临界条件下,得到了平原鹰嘴草的CO2提取物。采用气相色谱仪和质谱检测器测定提取物的成分分解。采用连续稀释法和圆盘扩散法测定其抑菌活性。采用三种微生物试验菌株:金黄色葡萄球菌ATCC 6538-P、大肠杆菌ATCC 8739、白色念珠菌ATCC 10231。结果。为了从planum L.的地下部分提取生物活性物质,我们选择了二氧化碳提取技术,这是一种处理二氧化碳(CO2)原料的技术,可以高浓度提取各种物质。二氧化碳萃取技术是分离药用植物原料中多种生物活性物质的一种有效且环保安全的方法。在平榆CO2萃取物的成分中鉴定出43种成分,其中α-亚麻酸含量最高,占8.30%;肉豆蔻酸,6.40%;石竹烯,6.92%;spatulous, 6.62%;以及其他主要已识别的化合物及其百分比。结论。研究表明,平胸羊奶CO2提取物中含有生物活性化合物,对大肠杆菌、金黄色葡萄球菌、白色念珠菌等临床重要微生物具有明显的抗菌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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