International Journal of Biomaterials最新文献

{"title":"Barriers Implementing Patient-Specific 3D-Printed Bone Scaffolds in Australia: A Scoping Review.","authors":"Anthony Vidler, Angus Hayes","doi":"10.1155/ijbm/5250806","DOIUrl":"10.1155/ijbm/5250806","url":null,"abstract":"<p><p>3D printing has emerged as an innovative technology over the last decade, with widespread uptake in several fields spanning science, technology and engineering; however, penetration into medical markets has been met with a variety of obstacles. At the 17^th Global Conference on Sustainable Manufacturing in 2018, medical technology stakeholders held a workshop to discuss the barriers that hinder the widespread adoption and diffusion of 3D printing technology in the Australian medical field. Patient-specific 3D-printed bone scaffolds were the central focus of this workshop, with five major barriers identified: material issues; manufacturing and postprocess approval; medical and professional endorsement and adoption; reimbursement; and staff training. To determine the progress made towards overcoming these barriers, we have reviewed the body of literature published from the 2018 conference until now. The most significant progress was observed in material issues with a significant increase in the number of materials and combination of materials that have been successfully printed and used in multiple animal trials and limited human cases. Manufacturing and postprocess approval issues that enable 3D-printed bone scaffold implantation have seen preliminary success with a handful of case trials documented with various levels of success, yet a distinct level of development is still required to satisfy this barrier before commercial production parameters and widespread adoption will be recognized. Medical and professional endorsement and adoption, reimbursement and staff training are yet to experience significant progression, with the possibility that the extent of these barriers will not be clearly understood until well after regulatory approval has been achieved.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"5250806"},"PeriodicalIF":3.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13051841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effect of Eugenol-Loaded Chitosan Nanoparticles Against <i>Helicobacter pylori</i> Infection: A Histologic and Molecular Study.","authors":"Mohammad Javad Tahmasebi, Mahmoud Osanloo, Alireaza Tavassoli, Abbas Abdollahi, Elham Zarenezhad, Fatemeh Norouzi, Behnoosh Miladpour","doi":"10.1155/ijbm/6699913","DOIUrl":"10.1155/ijbm/6699913","url":null,"abstract":"<p><strong>Background: </strong>Eugenol is a phenolic compound known for its antimicrobial, antifungal, and antioxidant properties. Its encapsulation in chitosan nanoparticles (EuChiNPs) enhances stability and therapeutic efficacy.</p><p><strong>Objectives: </strong>This study aimed to investigate the effects of EuChiNPs on <i>Helicobacter pylori (H. pylori</i>) infection in rats, focusing on its impact on inflammatory and apoptotic pathways.</p><p><strong>Methods: </strong>EuChiNPs were synthesized and characterized using DLS, TEM, and FTIR techniques. A total of 42 male Wistar rats were divided into six groups, including controls, <i>H. pylori</i>-infected, and treatment groups receiving EuChiNPs, standard antibiotics, or a combination. The effects of treatments were evaluated using RT-PCR, ELISA, Western blotting, and histopathology.</p><p><strong>Results: </strong>The combination of EuChiNPs and standard antibiotics significantly reduced the expression of inflammatory markers interleukin-1 beta (IL-1β) and interleukin-8 (IL-8). Serum levels of IL-1β, IL-8, and TNF-α were markedly decreased in the combination group compared with antibiotics alone (<i>p</i> < 0.0001). Western blot analysis revealed a reduction in caspase-3 and caspase-7 expression, indicating attenuation of apoptosis. Histopathological analysis showed improved mucosal integrity and reduced bacterial density in the combination treatment group.</p><p><strong>Conclusion: </strong>EuChiNPs demonstrated strong anti-inflammatory and antiapoptotic effects, especially when combined with standard antibiotics. This novel approach promises to improve <i>H. pylori</i> eradication while minimizing antibiotic resistance and side effects. Further clinical studies are recommended to validate these findings.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"6699913"},"PeriodicalIF":3.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13042343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Metallic Nanoparticles Applications in Neurological Disorders: A Review\".","authors":"","doi":"10.1155/ijbm/9871908","DOIUrl":"10.1155/ijbm/9871908","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/ijbm/4557622.].</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"9871908"},"PeriodicalIF":4.5,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147377184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selecting Hydrogel Films Composed of Carboxymethyl Cellulose and Microcrystalline Cellulose From OPEFB With Citric Acid as a Green Crosslinker for Fruit Wrapping.","authors":"Susi Susi, Makhmudun Ainuri, Wagiman Wagiman, Mohammad Affan Fajar Falah, Hisyam Musthafa Al Hakim","doi":"10.1155/ijbm/2806425","DOIUrl":"10.1155/ijbm/2806425","url":null,"abstract":"<p><p>Cellulose-based hydrogel films are promising alternatives to plastic for wrapping. Hydrogel films based on carboxymethyl cellulose (CMC) and microcrystalline cellulose (MCC) must exhibit high mechanical strength and be capable of absorbing moisture. Crosslinking increases the absorption capacity and mechanical strength of hydrogel films. As a filler, MCC can synergize with citric acid, which acts as an environmentally friendly crosslinker to enhance mechanical strength. This research aimed to develop a suitable CMC/MCC formulation and citric acid as a crosslinker, resulting in a hydrogel with high water absorption and mechanical strength. CMC/MCC formulations were combined with citric acid concentrations of 5%, 7.5%, and 10%. The results showed that the MCC and citric acid will affect absorption and mechanical strength. The addition of MCC up to 50% tends to produce a brittle hydrogel film, and this phenomenon is also correlated with increased citric acid. A 90:10 CMC/MCC formulation with 5% citric acid (w/v) resulted in a water uptake of 222.72 ± 9.32 at pH 7.0. In contrast, the 80:20 CMC/MCC formulation resulted in a water uptake of 603.02 ± 26.98. They both showed higher rehydration of the dry gel than the others. FTIR confirmed the sharpening of the peak wave number at 1705 cm^-1, which is identical to the protonated carbonyl group and correlates with the water absorption capacity. The morphology of hydrogel films containing a CMC/MCC ratio of 90:10 exhibits a smoother surface than that of those with a CMC/MCC ratio of 80:20, which feature bubbles on the surface cracks of the hydrogel film due to the presence of more water absorption channels. Hydrogel films with a CMC/MCC ratio of 90:10 and 5% citric acid (w/v) can be developed for wrapping by modifying their hygroscopic properties. In contrast, hydrogel films with an 80:20 ratio and 5% citric acid are suitable for use as absorbents.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"2806425"},"PeriodicalIF":3.5,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12911528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-Assisted Synthesis of Reduced Graphene Oxide Nanosheets From Graphene Oxide: Promising and Efficient Cytotoxic and Antidiabetic Potency in In Vitro, Kinetic, and In Silico Models.","authors":"Mansi Yadav, Divya Vashishth, Monika Bhardwaj, Jaya Parkash Yadav, Sudhir Kumar Kataria","doi":"10.1155/ijbm/5521416","DOIUrl":"10.1155/ijbm/5521416","url":null,"abstract":"<p><p>Green chemistry has recently made strides in the sustainable synthesis of next-generation nanomaterials using reducing agents sourced from plants. Biocompatible conversion of reduced graphene oxide from graphene oxide nanomaterials is of particular interest in medical applications. The leaf extract of <i>C. igneus</i> functions as a reducing agent to produce reduced graphene oxide nanosheets from its precursor graphene oxide. A variety of characterization techniques were employed to confirm the formation and stability of reduced graphene oxide nanosheets. The cytotoxicity and glucose uptake potential of nanosheets have been evaluated through in vitro cell-based assays. The MTT assay revealed a concentration-dependent cytotoxic effect on Chang liver and U87MG cells, with maximum 84.5% and 76.3% cell viability, respectively. Reduced graphene oxide exhibited a significant rise in glucose uptake, comparable to the metformin drug, with increasing concentration. Enhanced glucose transport mediated by the nanosheets resulted in increased glucose uptake. Effective IC₅₀ values of 84.46 μg·mL^-1 and 93.83 μg·mL^-1 were obtained in vitro enzyme inhibition studies against α-amylase and α-glucosidase, respectively. The enzyme kinetic investigation found noncompetitive inhibition for both enzymes. Molecular docking studies of <i>C. igneus</i> leaf derivatives were performed against α-amylase and α-glucosidase. Corosolic acid was selected based on favorable docking scores, binding interactions, and low root-mean-square deviation and was subjected to molecular dynamics simulations, which confirmed the stability of the resulting complex. This study concludes that reduced graphene oxide nanosheets derived from <i>C. igneus</i> leaves represent a novel antidiabetic agent that can reduce blood glucose levels and mitigate the complications associated with Type II diabetes.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"5521416"},"PeriodicalIF":3.5,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12911526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Genipin Crosslinking on the Cellular Delivery of Gelatin Nanocarriers Using Curcumin as a Payload.","authors":"Ram Pada Das, Minati Nayak, Beena Gobind Singh, Koushik Majee, Amit Kunwar","doi":"10.1155/ijbm/9512576","DOIUrl":"10.1155/ijbm/9512576","url":null,"abstract":"<p><p>Gelatin-based nanoformulations have received special attention for drug delivery applications because of their regulatory acceptability. A thorough understanding of the factors controlling the interaction of gelatin nanocarriers with cellular systems is crucial for their future biomedical applications. The present study addresses the effect of genipin crosslinking on the ability of gelatin nanoparticles (GNPs) to deliver curcumin, a pharmacologically active ingredient from turmeric into lung cancer (A549) cells. Briefly, the methodology was optimized to prepare GNP (15 mg/mL) crosslinked with 0.25, 0.5, and 1.0 mg/mL of genipin (GN-GNP1-3, respectively). The crosslinking of GN-GNP1-3 was established through UV-VIS, Fourier transform infrared spectroscopy, and circular dichroism measurements. Dynamic light scattering and transmission electron microscopy showed nearly identical hydrodynamic size (165 ± 15 nm) and shape (spherical) for GN-GNP1-3. Subsequently, these nanocarriers were loaded with curcumin and evaluated for drug delivery properties (loading efficiency and release kinetics), cellular uptake, cytotoxicity, and associated mechanisms. These studies together revealed that GN-GNP1-3 of increasing degree of crosslinking exhibited higher curcumin loading efficiency, facilitated slow and sustained release of curcumin over a prolonged period (80 h) by a non-Fickian mechanism, and ultimately increased the cellular uptake and the effectiveness (or cytotoxicity) of entrapped curcumin in A549 cells. The pharmacological abrogation investigations established that curcumin-loaded GN-GNP3 was internalized within A549 cells through caveolae-mediated endocytosis. In conclusion, genipin crosslinking of gelatin-based nanocarriers seemed to be a novel strategy to increase the cellular uptake cum effectiveness of a hydrophobic payload like curcumin.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"9512576"},"PeriodicalIF":3.5,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12905463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Moringa oleifera</i> Protein-Coated Gold Nanoparticles as Naturally Derived Disruptors of Biofilms.","authors":"Lakshmi Sudhir Menon, Narendranath Ghosh, Sumit Biswas","doi":"10.1155/ijbm/1381604","DOIUrl":"10.1155/ijbm/1381604","url":null,"abstract":"<p><strong>Background: </strong>Microbes having affinity to metallic surfaces develop a highly structured and sessile colony known as biofilms which poses significant challenges to the domain of implant surgery. Biofilm-mediated infections and the associated burden have prompted the search for multipronged approaches to tackle the problem. Gram-positive pathogens, most notably <i>Staphylococcus aureus</i>, has been known to colonize human tracts and form biofilms on implants and prosthetic devices. Antibiotic tolerance, drug efflux, and recalcitrance to the host immune response have added to the existing predicament of the biofilm infections.</p><p><strong>Objective: </strong>The search for inexpensive but effective avenues for combating biofilms has led to the use of metal nanoparticles conjugated with plant-derived proteins.</p><p><strong>Methods: </strong>In this study, a protein from <i>Moringa oleifera</i> leaf extract, p62, which has been previously identified to have antibiofilm properties, was conjugated with spherical gold nanoparticles (AuNPs) to target <i>S. aureus</i> biofilm formation.</p><p><strong>Results: </strong>The adsorption of p62 on the AuNPs was confirmed through microscopy, and the kinetics of binding was determined by plasmon resonance. The p62 coated AuNPs remained stable in solution and caused the successful disintegration of mature biofilms, more efficiently than the protein alone. The p62-AuNPs were also found to disrupt the morphology of the cocci and cause cell death as evidenced from the live/dead cell imaging through confocal microscopy. The protein and the nanoparticle were not cytotoxic to C2C12 human myoblast cell lines, affirming their suitability to be used on implants.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"1381604"},"PeriodicalIF":3.5,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12892876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146179376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocomposite Bone Scaffolds Based on Magnesium Alloy: A Detailed Investigation of Their In-Vitro Biodegradation Performance.","authors":"Adithya Garimella, Firoz Alam Faroque, Subrata Bandhu Ghosh, Sanchita Bandyopadhyay-Ghosh","doi":"10.1155/ijbm/9617232","DOIUrl":"10.1155/ijbm/9617232","url":null,"abstract":"<p><p>Polymeric biomaterials and their composites have been extensively explored for orthopaedic applications; however, their inadequate mechanical performance significantly restricts their use in load-bearing environments. Metallic biomaterials, by contrast, offer superior mechanical strength and structural stability. Among them, magnesium (Mg) has emerged as a particularly attractive candidate for temporary orthopaedic implants owing to its elastic modulus and density being close to those of natural bone, thereby minimising stress shielding. In addition, Mg inherently fulfils two critical requirements for orthopaedic implants-biocompatibility and biodegradability. In this study, a bioactive magnesium-alloy-based nanocomposite scaffold was engineered to overcome the limitations of conventional biomaterials while closely replicating the porous microarchitecture of human bone. A novel bioactive glass-ceramic, nano-fluorcanasite (n-FC), was incorporated into the Mg-alloy matrix to enhance osteogenic activity and accelerate bone tissue regeneration. The introduction of an interconnected porous structure was designed to promote efficient nutrient diffusion, facilitate metabolic waste removal and reduce implant density. Furthermore, the controlled addition of selected alloying elements in specific weight fractions effectively moderated the degradation kinetics of the Mg-based scaffold. The nanocomposite scaffolds were fabricated using a powder metallurgy route followed by sintering. Tailored porosity was achieved through the controlled incorporation of carbamide particles as a space-holding agent. The in vitro degradation behaviour of the scaffolds was systematically evaluated using a weight-change method after immersion in phosphate-buffered saline (PBS) for predetermined durations. The results demonstrate that, compared with unalloyed magnesium, the degradation rate of the nanocomposite scaffolds can be precisely and consistently regulated, highlighting their potential as mechanically competent, bioactive and biodegradable candidates for orthopaedic implant applications.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"9617232"},"PeriodicalIF":3.5,"publicationDate":"2026-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal Cocktail Hydrogel Film (<i>Garcinia mangostana</i>, <i>Centella asiatica</i>, and <i>Chromolaena odorata</i> Extracts): A Novel Wound Dressing Approach.","authors":"Tanikan Sangnim, Chonlada Panpipat, Suwisit Manmuan, Nontanat Leehueng, Wasutthanat Suphan, Chanapa Thuenaram, Kampanart Huanbutta","doi":"10.1155/ijbm/5576769","DOIUrl":"10.1155/ijbm/5576769","url":null,"abstract":"<p><p>Chronic and infected wounds remain a significant clinical challenge due to delayed healing and the risk of microbial contamination. Conventional wound dressings often fail to provide comprehensive therapeutic support, necessitating the development of advanced multifunctional materials. This study aimed to develop a multifunctional hydrogel wound dressing incorporating herbal extracts from <i>Garcinia mangostana</i>, <i>Centella asiatica</i>, and <i>Chromolaena odorata</i>, targeting key biological functions essential for wound healing. The novelty lies in the synergistic combination of these three extracts to address multistage healing needs and the systematic optimization of the polymer matrix via a design-of-experiments (DOE) approach. The extracts were obtained through optimized extraction techniques and quantified using HPLC, confirming the presence of bioactive markers. Pharmacological evaluations revealed distinct and synergistic activities: <i>Garcinia mangostana</i> extract showed potent antibacterial effects against <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> (MIC of 7.8 μg/mL) and strong antioxidant capacity (ABTS IC₅₀ of 26.5 μg/mL). In contrast, <i>Centella asiatica</i> extract demonstrated high biocompatibility on human keratinocytes (IC₅₀ of 736.11 μg/mL), while <i>Chromolaena odorata</i> provided significant anti-inflammatory and hemostatic benefits. A hydrogel base was developed using a polyvinyl alcohol (PVA)/sodium carboxymethyl cellulose (SCMC) polymer matrix, optimized via factorial design for texture, swelling ratio, and moisture retention. The extract-loaded hydrogel maintained skin-compatible pH, showed improved tensile strength and flexibility, and exhibited superior swelling capacity (188.7%) compared to the blank (120.3%). Overall, the formulated hydrogel demonstrates promise as an effective wound dressing with antibacterial, anti-inflammatory, and antioxidant properties, suitable for chronic or infected wound applications.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"5576769"},"PeriodicalIF":3.5,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12860223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Medicine Advancements: A Systematic Review on the Combinatory Effect of Platelet-Rich Plasma/Fibrin and Collagen.","authors":"Nunzia Gallo, Chiara Kodra, Domenico Rocco, Cosimo Saponaro, Alessandro Sannino, Luca Salvatore","doi":"10.1155/ijbm/1679626","DOIUrl":"10.1155/ijbm/1679626","url":null,"abstract":"<p><strong>Background: </strong>The regeneration of injured tissues remains a major clinical challenge. Among emerging biomaterials, collagen with platelet-rich plasma (PRP) or platelet-rich fibrin (PRF) showed promising outcomes, individually and in combination.</p><p><strong>Objective: </strong>To systematically review clinical evidence on the efficacy, applications, and safety of PRP/PRF and collagen for regenerative medicine applications.</p><p><strong>Methods: </strong>A systematic literature search was conducted in PubMed, Wiley Online Library, Google Scholar, and ClinicalTrials.gov (search date: September, 2025). Inclusion criteria: clinical studies evaluating PRP/PRF and collagen formulations. Exclusion: preclinical only or nonoriginal research. Data were synthesized narratively.</p><p><strong>Results: </strong>Twenty-six clinical studies were included. Applications included gingival recession, periodontitis, tendon injuries, bone regeneration, peripheral nerve repair, and chronic ulcers. Most studies reported positive outcomes, though many lacked control groups or had small sample sizes. No serious adverse events were reported.</p><p><strong>Conclusion: </strong>PRP/PRF and collagen show potential for various clinical applications in regenerative medicine. However, randomized clinical studies are necessary to demonstrate their superiority to standard treatment and to standardize protocols.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2026 ","pages":"1679626"},"PeriodicalIF":3.5,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12794683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}