Insect Science最新文献_第5页

Elasticity of trachea in the silkworm: A role of gene BmMuc91C. 家蚕气管弹性：BmMuc91C基因的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-01-16 DOI: 10.1111/1744-7917.13492

Yifei Chen, Haonan Dong, Chunxia Xiao, Qinglang Wang, Jing Gong, Yong Hou

{"title":"Elasticity of trachea in the silkworm: A role of gene BmMuc91C.","authors":"Yifei Chen, Haonan Dong, Chunxia Xiao, Qinglang Wang, Jing Gong, Yong Hou","doi":"10.1111/1744-7917.13492","DOIUrl":"https://doi.org/10.1111/1744-7917.13492","url":null,"abstract":"Respiration is a vital process essential for organism survival, with most terrestrial insects relying on a sophisticated tubular tracheal network. In the current study, a gene with repetitive sequence was identified within the silkworm genome. Designated as BmMuc91C, it contains a dozen repeated motifs \"PSSSYGAPX\" and \"GGYSSGGX\" in its sequence. BmMuc91C exhibits specific expression in the tracheal system of silkworm larvae, with significantly higher expression levels during the molting stage. Overexpression of BmMuc91C in individual silkworms resulted in a marked increase in tracheal diameter, particularly during the molting stage. Immunofluorescence staining using a BmMuc91C antibody revealed a noticeable thickening of the apical extracellular matrix in the trachea. Tensile testing confirmed a considerable enhancement in tracheal elasticity. Additionally, a BmMuc91C mutation strain of silkworms was generated using the clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 system. Although no significant differences were observed in the growth, development, and molting of BmMuc91C mutant silkworms, mechanical tests demonstrated a decrease in tracheal elasticity. Transcriptomic techniques revealed that a significant number of cuticular and chitin-binding proteins were among the differentially expressed genes between mutant and wild-type silkworms. Furthermore, the recombined BmMuc91C protein was successfully expressed using the Escherichia coli system. Cross-linking experiments with horseradish peroxidase demonstrated the formation of macromolecular complexes of BmMuc91C, which exhibited spontaneous luminescent properties under ultraviolet light. This research sheds light on the role of elastic proteins in insect tracheae and provides valuable insights for the development of elastic biomaterials.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Winter fruit contribution to the performance of the invasive fruit fly Drosophila suzukii under different thermal regimes. 不同温度条件下冬季果实对入侵果蝇铃木果蝇性能的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-01-16 DOI: 10.1111/1744-7917.13494

Jordy Larges, Gwenaëlle Deconninck, Romain Ulmer, Vincent Foray, Nathalie Le Bris, Marion Chorin, Hervé Colinet, Olivier Chabrerie, Patrice Eslin, Aude Couty

{"title":"Winter fruit contribution to the performance of the invasive fruit fly Drosophila suzukii under different thermal regimes.","authors":"Jordy Larges, Gwenaëlle Deconninck, Romain Ulmer, Vincent Foray, Nathalie Le Bris, Marion Chorin, Hervé Colinet, Olivier Chabrerie, Patrice Eslin, Aude Couty","doi":"10.1111/1744-7917.13494","DOIUrl":"https://doi.org/10.1111/1744-7917.13494","url":null,"abstract":"Polyphagous insect species develop using multiple host plants. Often considered beneficial, polyphagy can also be costly as host nutritional quality may vary. Drosophila suzukii (Matsumura) is an invasive species that can develop on numerous fruit species over the annual cycle. Here, we assessed the contribution of winter-available fruit to the development of seasonal populations of D. suzukii, under fluctuating late winter/early spring temperature regimes. We infested an artificial diet and three suitable fruit species available in winter/early spring (Aucuba japonica, Elaeagnus ×submacrophylla, Viscum album) with D. suzukii larvae under three temperature regimes: constant 20 °C, fluctuating controlled regime of 8-15 °C (12 h of light at 8 °C and 12 h of dark at 15 °C), and uncontrolled outdoor regime during spring. As expected, fly performance was impaired by early spring-like environmental conditions, whatever the development diet, and the winter fruit were suboptimal diets compared to the artificial diet, whatever the thermal regime. However, under cold fluctuating temperature regimes, the ranking of fruit supporting the best performance changed, highlighting the occurrence of physiological trade-offs. Winter-acclimated females preferentially oviposited in A. japonica and/or E. ×submacrophylla, whatever the thermal regime, which does not support the preference-performance hypothesis. This finding is also discussed in the context of D. suzukii management strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using artificial neural networks to explain the attraction of jewel beetles (Coleoptera: Buprestidae) to colored traps. 利用人工神经网络解释宝石甲虫（鞘翅目：金龟子科）对彩色陷阱的吸引力。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-01-16 DOI: 10.1111/1744-7917.13496

Roger D Santer, Otar Akanyeti

{"title":"Using artificial neural networks to explain the attraction of jewel beetles (Coleoptera: Buprestidae) to colored traps.","authors":"Roger D Santer, Otar Akanyeti","doi":"10.1111/1744-7917.13496","DOIUrl":"https://doi.org/10.1111/1744-7917.13496","url":null,"abstract":"Jewel beetles pose significant threats to forestry, and effective traps are needed to monitor and manage them. Green traps often catch more beetles, but purple traps catch a greater proportion of females. Understanding the function and mechanism of this behavior can provide a rationale for trap optimization. Jewel beetles possess UV-, blue-, green-, and red-sensitive photoreceptors, and perceive color differently from humans. Jewel beetle photoreceptor signals were calculated for tree leaf and tree bark stimuli, representing feeding and oviposition sites of adult jewel beetles respectively. Artificial neural networks (ANNs) were trained to discriminate those stimuli using beetle photoreceptor signals, providing in silico models of the neural processing that might have evolved to drive behavior. ANNs using blue-, green-, and red-sensitive photoreceptor inputs could classify these stimuli with very high accuracy (>99%). ANNs processed photoreceptor signals in an opponent fashion: increasing green-sensitive photoreceptor signals promoted leaf classifications, while increasing blue- and red-sensitive photoreceptor signals promoted bark classifications. Trained ANNs were fed photoreceptor signals calculated for traps, wherein they always classified green traps as leaves, but often classified purple traps as bark, indicating that these traps share salient features with different classes of tree stimuli from a beetle's eye view. A metric representing the photoreceptor opponent mechanism implicated by ANNs then explained catches of emerald ash borer, Agrilus planipennis, at differently colored traps from a previous field study. This analysis provides a hypothesized behavioral mechanism that can now guide the rational selection and improvement of jewel beetle traps.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinematics and directionality of body turning in water striders (Gerris argentatus) on the water surface. 水黾（Gerris argentatus）在水面上身体转动的运动学和方向性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-01-06 DOI: 10.1111/1744-7917.13486

Javad Meshkani, Hamed Rajabi, Alexander Kovalev, Stanislav N Gorb

{"title":"Kinematics and directionality of body turning in water striders (Gerris argentatus) on the water surface.","authors":"Javad Meshkani, Hamed Rajabi, Alexander Kovalev, Stanislav N Gorb","doi":"10.1111/1744-7917.13486","DOIUrl":"https://doi.org/10.1111/1744-7917.13486","url":null,"abstract":"Water striders inhabit the elastic surface tension film of water, sharing their environment with other aquatic organisms. Their survival relies heavily on swift maneuverability and navigation around floating obstacles, which aids in the exploration of their habitat and in escaping from potential threats. Their high agility is strongly based on the ability to execute precise turns, enabling effective directional control. This paper investigates the intricate coordination of leg movements essential for initiating and sustaining turning maneuvers in water striders. We elucidate the distinct roles of each leg in modulating posture and stability during turns, with a focus on the pivotal role of the midlegs in maintaining directional movement. Through analysis of leg accelerations, decelerations, and load distribution, we unveil the spatiotemporal dynamics governing successful turns. Our findings reveal refined turning strategies employed by water striders in varying situations, from narrow to wide turns, characterized by adaptations in their locomotor system, particularly in the widening of the sculling field. Additionally, we report the phenomenon of reverse sculling, a novel escape tactic of water striders. By shedding light on the maneuverability of water striders, this study contributes to a deeper understanding of animal locomotion strategies in aquatic environments.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of sulfakinin and its role in larval feeding and molting in Spodoptera frugiperda. 磺胺氰素的鉴定及其在果夜蛾幼虫取食和蜕皮中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-01-06 DOI: 10.1111/1744-7917.13480

Ming-Qing Yu, Jun-Hong Linghu, Hua-Yan Xie, Gang Li, Feng Zhu, Guy Smagghe, Shun-Hua Gui, Tong-Xian Liu

{"title":"Characterization of sulfakinin and its role in larval feeding and molting in Spodoptera frugiperda.","authors":"Ming-Qing Yu, Jun-Hong Linghu, Hua-Yan Xie, Gang Li, Feng Zhu, Guy Smagghe, Shun-Hua Gui, Tong-Xian Liu","doi":"10.1111/1744-7917.13480","DOIUrl":"https://doi.org/10.1111/1744-7917.13480","url":null,"abstract":"Feeding and molting are particularly important physiological processes for insects, and it has been reported that neuropeptides are involved in the nervous regulation of these 2 processes. Sulfakinin (SK) is an important neuropeptide that is widely distributed among insects and plays a pivotal role in regulating feeding, courtship, aggression, and locomotion. In this study, we investigated the involvement of SK in feeding and molting on a highly notorious pest insect, the fall armyworm, Spodoptera frugiperda. SK transcript levels were found in all larval stages and there was a predominant expression of SK in the brain of 5th instar larvae. By immunostaining, SK was detected in 2 pairs of cells in the median protocerebrum. But during prolonged periods of starvation, there was a significant reduction in SK messenger RNA levels; however, subsequent refeeding led to a notable increase. To investigate the role of SK in feeding and molting, SK was silenced in S. frugiperda larvae through RNA interference. This resulted in a significant increase in food intake, weight gain, and the molting process happened more rapidly in the double-stranded SK-treated larvae compared to the controls. Conversely, injection of sulfated SK peptide (sSK) caused opposite effects. Interestingly, SK-knockdown in larvae resulted in increased levels of 20-hydroxyecdysone and also of the expression of some of it signaling pathway genes. Altogether, this study highlights the important role played by SK in regulating feeding and molting in S. frugiperda.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elongator subunit Elp3 regulates reproduction in Tribolium castaneum by interacting with FOXO. 伸长亚基Elp3通过与FOXO相互作用调控三角藓的繁殖。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-01-02 DOI: 10.1111/1744-7917.13487

Huichen Ge, Jiaping Wei, Daojie Guan, Zhichao Wang, Hai Li, Hainan Zhang, Kun Qian, Jianjun Wang

{"title":"Elongator subunit Elp3 regulates reproduction in Tribolium castaneum by interacting with FOXO.","authors":"Huichen Ge, Jiaping Wei, Daojie Guan, Zhichao Wang, Hai Li, Hainan Zhang, Kun Qian, Jianjun Wang","doi":"10.1111/1744-7917.13487","DOIUrl":"https://doi.org/10.1111/1744-7917.13487","url":null,"abstract":"As the catalytic subunit of the Elongator complex, Elongator protein 3 (Elp3) plays a crucial role in multiple physiological processes, including growth, development and immune responses. Previous studies on Elp3 have focused on Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens (human) or Mus musculus (mouse), whereas there are few reports on Elp3 in agricultural pests. Here, the role of TcElp3 in reproduction in the red flour beetle, Tribolium castaneum, was investigated, and the underlying mechanisms were explored. The results showed that RNA interference (RNAi)-mediated knockdown of TcElp3 in female pupae led to decreased fecundity in female adults. Consistently, the injection of dsTcElp3 into female pupae decreased the mRNA levels of the vitellogenin (Vg)-encoding genes TcVg1 and TcVg2 in female adults. Notably, knockdown of TcElp3 upregulated the expression of forkhead box protein O (FOXO) at both the mRNA and the protein level in T. castaneum, and promoted the nuclear translocation of TcFOXO. Additionally, TcElp3 directly interacts with TcFOXO and the silencing of TcElp3 significantly decreased the acetylation level of TcFOXO. Overall, our studies reveal that Elp3 regulates beetle reproduction by interacting with FOXO and modulating its acetylation status.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in blowfly (Diptera: Calliphoridae) wing morphology during succession in rat carcasses across forest and grassland habitats in South Brazil. 巴西南部森林和草原大鼠尸体演替过程中苍蝇（双翅目：蛱蝶科）翅膀形态的变化。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-31 DOI: 10.1111/1744-7917.13485

Lucas Ferreira Colares, Anita da Silva Herdina, Mariana Bender, Cristian de Sales Dambros

{"title":"Changes in blowfly (Diptera: Calliphoridae) wing morphology during succession in rat carcasses across forest and grassland habitats in South Brazil.","authors":"Lucas Ferreira Colares, Anita da Silva Herdina, Mariana Bender, Cristian de Sales Dambros","doi":"10.1111/1744-7917.13485","DOIUrl":"https://doi.org/10.1111/1744-7917.13485","url":null,"abstract":"Succession is one of the most extensively studied ecological phenomena, yet debates persist about the importance of dispersal and external factors in driving this process. We aimed to quantify the influence of these factors by investigating how wing-related traits evolve across succession of blowfly (Diptera: Calliphoridae) communities in South Brazil. Rat carrion was placed in both forest and grassland habitats, and the associated blowfly communities were documented throughout the decomposition process. Using morphometric analysis, we measured wing and thorax traits and assessed trait changes over succession through mixed models. Our findings revealed that carrion succession follows distinct trajectories in forest and grassland environments. Specifically, we observed that Calliphora lopesi predominantly visited carcasses during the final phase of decomposition, resulting in significant differences in species composition and wing size between habitats. In forests, wing size increased toward the later stages of succession, whereas an opposite trend was observed in grasslands. Notably, these trait patterns were only evident at the species level, indicating that intraspecific trait variation is irrelevant. Stronger dispersers tend to arrive during the later stages of succession, suggesting that dispersal has a negligible role in shaping successional dynamics. Instead, environmental differences between habitats drive trait patterns throughout succession. Our results suggest that community composition in ephemeral resources is governed by deterministic processes and that successional stages can be predicted based on blowfly wing traits. Specifically, the presence of the large-winged C. lopesi indicates late decay, while the small-winged Chrysomia albiceps and Lucilia eximia are indicative of early decay.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two secretory T2 RNases from a fungal pathogen target distinct insect cell transmembrane proteins to cause cytotoxicity. 来自真菌病原体的两种分泌性T2 rnase针对不同的昆虫细胞跨膜蛋白引起细胞毒性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-22 DOI: 10.1111/1744-7917.13488

Yong Yue, Xin Zhao, Zhuoyue Lu, Wei Dou, Zhibing Luo, Kangmin Lei, Dan Xu, Yongjun Zhang

{"title":"Two secretory T2 RNases from a fungal pathogen target distinct insect cell transmembrane proteins to cause cytotoxicity.","authors":"Yong Yue, Xin Zhao, Zhuoyue Lu, Wei Dou, Zhibing Luo, Kangmin Lei, Dan Xu, Yongjun Zhang","doi":"10.1111/1744-7917.13488","DOIUrl":"https://doi.org/10.1111/1744-7917.13488","url":null,"abstract":"Fungal pathogens produce secretory ribonuclease (RNase) T2 proteins during infection, which contribute to fungal virulence via their enzyme functions in degradation of host cell RNA. However, the details of those proteins entering the host cells are unclear. Our previous study demonstrated that the two secretory RNase T2 members, BbRNT2 and BbTrv, produced by the insect fungal pathogen Beauveria bassiana, caused cytotoxic damage to insect cells and contributed to fungal virulence. Here, the Spodoptera frugiperda ovarian epithelial cells (sf9 cells) were used as models to investigate the interactions of the two fungus-produced RNase T2 proteins with the insect cells. Two transmembrane proteins, an ABC transporter (SfABCG) and an Innexin 7-like protein (Sfinx), were identified from the sf9 cells as interacting with BbRNT2 and BbTrv, respectively, through protein immunoprecipitation, yeast-two hybrid tests and protein pull-down assays. Although a slight decrease in the sf9 cell viability was examined by transfection of RNA interference of SfABCG or Sfinx, the transfected cells displayed a dramatically decreased sensitivity to BbRNT2 or BbTrv, suggesting the requirement of the two transmembrane proteins for BbRNT2 and BbTrv to enter the insect cells. These results reveal a mechanism of the cytotoxic molecules, T2 RNases, produced by the fungal pathogen, entering the insect cells via interaction with specific insect cell transmembrane proteins and causing cytotoxic damage.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NF-κB/Relish readjusts miR-100 expression and recovers immune homeostasis in Drosophila melanogaster. NF-κB/Relish 重新调整 miR-100 的表达，恢复黑腹果蝇的免疫平衡。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-17 DOI: 10.1111/1744-7917.13484

Xiaolong Yao, Lu Lin, Zifeng Ye, Miaomiao Huo, Ping Jin, Fei Ma

{"title":"NF-κB/Relish readjusts miR-100 expression and recovers immune homeostasis in Drosophila melanogaster.","authors":"Xiaolong Yao, Lu Lin, Zifeng Ye, Miaomiao Huo, Ping Jin, Fei Ma","doi":"10.1111/1744-7917.13484","DOIUrl":"https://doi.org/10.1111/1744-7917.13484","url":null,"abstract":"The regulation and maintenance of immune homeostasis are essential for animal survival, but the molecular mechanisms are not fully understood. Here, we used the model organism Drosophila melanogaster to uncover a potential mechanism by which the nuclear factor-κB transcription factor Relish and miR-100 cooperatively regulate innate immune homeostasis. We first demonstrated in vitro and in vivo that miR-100 can negatively regulate the immune responses of the Imd pathway by inhibiting the expression of TAK1-associated binding protein 2 (Tab2) gene. Second, we found that Relish, an important transcription factor in the Drosophila Imd pathway, could not only modulate the expressions of antimicrobial peptides (AMPs) to promote immune responses, but also bind to the promoter region of miR-100 and activate its transcription to inhibit immune responses. Third, the dynamic expression of genes profiling indicated that the Relish/miR-100/Tab2 regulatory axis could contribute to innate immune homeostasis in Drosophila. Together, our findings reveal the dual role of Relish in immune regulation, that is, Relish promotes the expression of AMPs to resist pathogen infection in the early immune response, while in the late immune stages, Relish readjusts the expression of miR-100 to negatively control immune responses to avoid excessive immunity thus maintaining immunohomeostasis. Meanwhile, our study provides a new perspective for further understanding the complex regulatory mechanism of immune homeostasis in animals.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142835617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Juvenile hormone induces phosphorylation of insulin/insulin-like growth factor signaling proteins in previtellogenic Aedes aegypti mosquitoes. 幼代激素诱导致卵前埃及伊蚊胰岛素/胰岛素样生长因子信号蛋白磷酸化

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-11 DOI: 10.1111/1744-7917.13482

Wenhao Zhao, Pengcheng Liu, Thomas R Saunders, Jinsong Zhu

{"title":"Juvenile hormone induces phosphorylation of insulin/insulin-like growth factor signaling proteins in previtellogenic Aedes aegypti mosquitoes.","authors":"Wenhao Zhao, Pengcheng Liu, Thomas R Saunders, Jinsong Zhu","doi":"10.1111/1744-7917.13482","DOIUrl":"https://doi.org/10.1111/1744-7917.13482","url":null,"abstract":"Juvenile hormone (JH) plays a pivotal role in regulating post-emergence development and metabolism in previtellogenic female Aedes aegypti mosquitoes. In contrast, yolk protein precursor production and egg maturation after a blood meal are regulated by the steroid hormone 20-hydroxyecdysone, the insulin-like growth factor (IGF)/insulin signaling (IIS) pathway, and the mammalian target of rapamycin (mTOR) pathway. The role of IIS/mTOR signaling in female adults prior to blood feeding has not been thoroughly investigated. In this study, we identified a significant increase in the phosphorylation of key effector proteins in the IIS/mTOR signaling pathway, including eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), ribosomal protein S6 kinase (S6K) and forkhead box protein O1 (FoxO1), in previtellogenic females. In vitro fat body culture experiments suggest that JH induces these phosphorylations through rapid nongenomic signaling mediated by the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/mTOR network. RNA interference experiments demonstrated that activation of IIS/mTOR signaling in previtellogenic females modulate metabolic gene expression, promoting the accumulation of energy reserves (glycogen and triglycerides), which influence mosquito fecundity. Additionally, depletion of either the insulin receptor (InR) or the JH receptor Methoprene-tolerant (Met) in adult mosquitoes abolished the phosphorylation of these proteins, indicating that both receptors are involved in JH-induced membrane-initiated signal transduction. Although the precise mechanisms remain unclear, this study uncovers a novel function of the IIS/mTOR pathway in adult mosquitoes before blood feeding, as well as a new mode of JH action through its crosstalk with the IIS pathway.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0