Insect Science最新文献_第5页

Identification and function of microRNAs in hemipteran pests: A review 半翅目害虫体内微RNA的鉴定和功能：综述

IF 4 1区农林科学

Insect Science Pub Date : 2024-09-18 DOI: 10.1111/1744-7917.13449

Yipeng Ren, Wenhao Dong, Juhong Chen, Huaijun Xue, Wenjun Bu

{"title":"Identification and function of microRNAs in hemipteran pests: A review","authors":"Yipeng Ren, Wenhao Dong, Juhong Chen, Huaijun Xue, Wenjun Bu","doi":"10.1111/1744-7917.13449","DOIUrl":"https://doi.org/10.1111/1744-7917.13449","url":null,"abstract":"Hemiptera is one of the most significant orders of insect pests, including whiteflies, true bugs, aphids, planthoppers, psyllids, and so forth, which have led to substantial economic losses in agricultural industries and have significantly affected food yields through their ability to suck the phloem sap of crops and transmit numerous bacterial and viral pathogens. Therefore, explorations of pest‐specific, eco‐friendly and easy‐to‐adopt technologies for hemipteran pest control are urgently needed. To the best of our knowledge, microRNAs (miRNAs), which are endogenous non‐coding small RNAs approximately 22 nucleotides in length, are involved in regulating gene expression via the direct recognition and binding of the 3′‐untranslated region (3′‐UTR) of target messenger RNAs (mRNAs) or by acting as a center of a competitive endogenous RNA (ceRNA) network at the post‐transcriptional level. This review systematically outlines the characterization and functional investigation of the miRNA biogenesis pathway in hemipteran pests, such as whiteflies, true bugs, aphids and planthoppers. In addition, we explored the results of small RNA sequencing and functional observations of miRNAs in these pests, and the results suggest that the numerous miRNAs obtained and annotated via high‐throughput sequencing technology and bioinformatic analyses contribute to molting development, fitness, wing polyphenism, symbiont interactions and insecticide resistance in hemipteran pests. Finally, we summarize current advances and propose a framework for future research to extend the current data and address potential limitations in the investigation and application of hemipteran miRNAs.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori. BmEL-2通过调控BmAGPATγ和BmFAF2的表达来促进桑蚕体内甘油三酯的代谢。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-15 DOI: 10.1111/1744-7917.13446

Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun

{"title":"BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori.","authors":"Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun","doi":"10.1111/1744-7917.13446","DOIUrl":"https://doi.org/10.1111/1744-7917.13446","url":null,"abstract":"Bombyx mori ELAV-like-1 (BmEL-1) and B. mori ELAV-like-2 (BmEL-2) are 2 members of the ELAV-like family of RNA-binding proteins. Mutations in Bmel-1 and Bmel-2 resulted in 5.8% and 28.5% decreases in larval weight on the 3rd day of the 5th instar larva (L5D3), respectively. Triglycerides (TG) are the most important energy resource and are the main component of neutral fat (NF) in animals. To investigate the role of Bmelav-like genes in the synthesis and decomposition of TG, transcriptomic, and metabolic analyses were performed on the whole bodies on the 1st day of the 2nd instar larvae (L2D1) and on fat bodies on L5D3 of Bmel-1- and Bmel-2- mutants, respectively. As compared with the control silkworm, differentially expressed genes generated in both mutants were mainly enriched in lysine degradation, fatty acid (FA) metabolism, and unsaturated FAs biosynthesis. The diglyceride and phosphatide contents were significantly lower in Bmel-1- and Bmel-2- fat bodies than those of the control group. Consistently, the NF content of both mutants' fat bodies were reduced by 50% and 60%, respectively. BmEL-2 positively regulates BmAGPATγ (B. mori 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, LOC101741736) and BmFaF2 (B. mori fatty acid synthetase-associated factor 2, LOC101739090) expression by binding to the specific regions of their 3' untranslated regions in BmN cells. This study suggests that BmEL-2 may be an important regulator of BmAGPATγ and BmFAF2 expression and thereby participates in TG metabolism in the silkworm fat body.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytoplasma infection renders cranberries more susceptible to above- and belowground insect herbivores. 植原体感染使蔓越莓更容易受到地上和地下昆虫食草动物的侵害。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-15 DOI: 10.1111/1744-7917.13444

Cesar Rodriguez-Saona, Paolo Salazar-Mendoza, Robert Holdcraft, James Polashock

{"title":"Phytoplasma infection renders cranberries more susceptible to above- and belowground insect herbivores.","authors":"Cesar Rodriguez-Saona, Paolo Salazar-Mendoza, Robert Holdcraft, James Polashock","doi":"10.1111/1744-7917.13444","DOIUrl":"https://doi.org/10.1111/1744-7917.13444","url":null,"abstract":"While phytoplasma infections in plants are known to affect their interactions with aboveground herbivores, the impact of different genotypes on these infections and their effects on belowground herbivores remains largely unexplored. In cranberry (Vaccinium macrocarpon), infection by the phytoplasma Candidatus Phytoplasma sp. subgroup 16SrIII-Y leads to false blossom disease. This study investigates whether cranberry infection by this phytoplasma affects the performance and feeding behavior of a foliar feeder (spongy moth, Lymantria dispar) and a root feeder (oriental beetle, Anomala orientalis). Using phytoplasma-infected and uninfected cranberries of two genotypes (\"Ben Lear\" and \"Crimson Queen\"), the survival, growth and consumption of L. dispar and A. orientalis larvae were measured. To assess the effects on plant morphological and chemical traits, we also examined the impact of phytoplasma infection on shoot and root growth, carbon and nitrogen content, and the levels of defensive compounds such as proanthocyanidins (PACs). Results indicate that larvae of L. dispar and A. orientalis generally showed larger size and more efficient tissue consumption on infected plants, with these effects varying by cranberry genotype, possibly due to differences in phytoplasma titer. Phytoplasma infection was associated with stunted growth, elevated nitrogen content, and lower PAC levels in both shoots and roots of infected cranberry plants compared to uninfected ones. These findings indicate that phytoplasma infection potentially manipulates plant chemical composition by increasing nutrient levels and decreasing defensive compounds, enhancing herbivore performance both above and belowground. This study sheds light on the intricate interplay among plants, phytoplasma infection, and insect herbivore communities.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR‐927 regulates insect wing development by targeting the Hippo pathway miR-927 通过靶向 Hippo 通路调控昆虫翅膀的发育

IF 4 1区农林科学

Insect Science Pub Date : 2024-09-10 DOI: 10.1111/1744-7917.13445

Xuan Yu, Bing Sun, Xuequan Gao, Qingxin Liu, Zizhang Zhou, Yunhe Zhao

{"title":"miR‐927 regulates insect wing development by targeting the Hippo pathway","authors":"Xuan Yu, Bing Sun, Xuequan Gao, Qingxin Liu, Zizhang Zhou, Yunhe Zhao","doi":"10.1111/1744-7917.13445","DOIUrl":"https://doi.org/10.1111/1744-7917.13445","url":null,"abstract":"How organ size is determined is a fundamental question in life sciences. Recent studies have highlighted the importance of the Hippo pathway in regulating organ size. This pathway controls cell proliferation and cell death to maintain the proper number of cells. The activity of the Hippo pathway is tightly fine‐tuned through various post‐translational modifications, such as phosphorylation and ubiquitination. Here, we discover that miR‐927 is a novel regulator of wing size. Overexpression of miR‐927 decreases wing size, which can be rescued by co‐expressing miR‐927‐sponge. Next, we show that miR‐927 stimulates apoptosis and suppresses the expression of Drosophila inhibitor of apoptosis protein 1, a well‐known target gene of the Hippo pathway. Genetic epistatic analyses position miR‐927 upstream of Yorkie (Yki) to modulate the Hippo pathway. In addition, there is a matching miR‐927 seed site in the yki 3′ untranslated region (3′‐UTR), and we demonstrate that yki 3′‐UTR is the direct target of miR‐927. Ultimately, our study reveals that the targeting of yki by miR‐927 to regulate the Hippo pathway is conserved in Helicoverpa armigera. Administration of miR‐927 via star polycation (SPc) nanocarrier effectively inhibits wing development in H. armigera. Taken together, our findings uncover a novel mechanism by which Yki is silenced at the post‐transcriptional level by miR‐927, and provide a new perspective on pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The beetle's structural protein CPCFC making elytra tough and rigid. 甲虫的结构蛋白 CPCFC 使蜕皮坚硬无比。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-05 DOI: 10.1111/1744-7917.13443

Han Bao, Yuantao Liu, Yanwei Duan, Lei Chen, Qing Yang

{"title":"The beetle's structural protein CPCFC making elytra tough and rigid.","authors":"Han Bao, Yuantao Liu, Yanwei Duan, Lei Chen, Qing Yang","doi":"10.1111/1744-7917.13443","DOIUrl":"https://doi.org/10.1111/1744-7917.13443","url":null,"abstract":"The insect cuticle, which serves as both a protective barrier and an efficient lever system for locomotion, is an extracellular matrix primarily composed of chitin and protein. The cuticle protein CPCFC characterized by a \"CFC\" motif containing 2 Cys split by the insertion of 5 residues is distributed across most insect species and specifically localized in the hard part of the cuticle. However, their physiological function is not fully understood. Here, we report 2 CPCFC proteins, TcCPCFC1 and TcCPCFC2, derived from the Coleopteran insect Tribolium castaneum. We revealed that TcCPCFC1 and TcCPCFC2 were predominantly expressed during the larval and adult stages of T. castaneum, respectively. The transcription downregulation of TcCPCFC1 significantly decreased the modulus and toughness of the elytral cuticle. We found that TcCPCFC proteins have high binding affinity to chitin. We cloned and produced recombinant TcCPCFC proteins and demonstrated that the addition of TcCPCFC proteins to chitin hydrogel greatly enhanced the hydrogel's modulus and toughness by forming denser chitin fibrous networks. Our findings reveal the functional role of CPCFC proteins in enhancing mechanical properties of insect cuticle, and we validate this process in vitro, and offer a protein candidate for fabrication of advanced chitin-based materials.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application. 表达生物活性人类睫状神经营养因子的转基因家蚕在生物医学中的应用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-01 DOI: 10.1111/1744-7917.13442

Weiqun Lan, Wenjing Geng, Xuechun Jiang, Feng Chen, Mingyi Zhou, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li

{"title":"Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application.","authors":"Weiqun Lan, Wenjing Geng, Xuechun Jiang, Feng Chen, Mingyi Zhou, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li","doi":"10.1111/1744-7917.13442","DOIUrl":"https://doi.org/10.1111/1744-7917.13442","url":null,"abstract":"Ciliary neurotrophic factor (CNTF) acts as a potent neuroprotective agent in neuronal survival and regeneration, and can also induce the differentiation of several stem cells into neurons, which highlights the broad application of CNTF in biomedicine. However, large-scale production of bioactive recombinant human CNTF protein remains to be explored. Herein, this study aims to express a bioactive human CNTF protein on a large scale by genetically engineering a silk gland bioreactor of silkworm. Our results showed that CNTF protein was successfully expressed in the middle silk gland (MSG) of silkworm, which can be secreted into the silks with the amount of 3.2 mg/g cocoons. The fabrication of human CNTF-functionalized silk material was able to promote proliferation and migration of neural cells when compared to the natural silk protein. Importantly, this functional silk material could also facilitate neurite outgrowth of mouse retinal ganglion cell (RGC-5) cells. All these data demonstrated a high bioactivity of the recombinant human CNTF protein expressed in the MSG of silkworm. The further fabrication of different silk materials with CNTF bioactivity will give biomedical applications in tissue engineering and neuroregeneration.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142106999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis and functional study of phospholipase A₂ in Galleria mellonella larvae lipid metabolism in response to envenomation by an ectoparasitoid, Iseropus kuwanae. 转录组分析和磷脂酶 A2 在幼虫脂质代谢中的功能研究，以应对外寄生虫 Iseropus kuwanae 的螫伤。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-01 DOI: 10.1111/1744-7917.13440

Hanqi Zhu, Xinhao Liang, Jianhao Ding, Jinzheng Wang, Ping Li, Weihong Zhou, Jun Wang, Fu-An Wu, Sheng Sheng

{"title":"Transcriptome analysis and functional study of phospholipase A2 in Galleria mellonella larvae lipid metabolism in response to envenomation by an ectoparasitoid, Iseropus kuwanae.","authors":"Hanqi Zhu, Xinhao Liang, Jianhao Ding, Jinzheng Wang, Ping Li, Weihong Zhou, Jun Wang, Fu-An Wu, Sheng Sheng","doi":"10.1111/1744-7917.13440","DOIUrl":"https://doi.org/10.1111/1744-7917.13440","url":null,"abstract":"There is abundant evidence that parasitoids manipulate their hosts by envenomation to support the development and survival of their progeny before oviposition. However, the specific mechanism underlying host nutritional manipulation remains largely unclear. To gain a more comprehensive insight into the effects induced by the gregarious ectoparasitoid Iseropus kuwanae (Hymenoptera: Ichneumonidae) on the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) larvae, we sequenced the transcriptome of both non-envenomed and envenomed G. mellonella larvae, specifically targeting genes related to lipid metabolism. The present study revealed that 202 differentially expressed genes (DEGs) were identified and 9 DEGs were involved in lipid metabolism. The expression levels of these 9 DEGs relied on envenomation and the duration post-envenomation. Further, envenomation by I. kuwanae induced an increase in triglyceride (TG) level in the hemolymph of G. mellonella larvae. Furthermore, silencing GmPLA2 in G. mellonella larvae 24 h post-envenomation significantly decreased the content of 4 unsaturated fatty acids and TG levels in the hemolymph. The content of linoleic acid and α-linoleic acid were significantly decreased and the content of oleic acid was significantly increased by exogenous supplement of arachidonic acid. Meanwhile, the reduction in host lipid levels impairs the growth and development of wasp offspring. The present study provides valuable knowledge about the molecular mechanism of the nutritional interaction between parasitoids and their hosts and sheds light on the coevolution between parasitoids and host insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142106998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of the insect adult fat body relies on glycolysis, lipid synthesis, cell proliferation, and cell adhesion. 昆虫成虫脂肪体的发育依赖于糖酵解、脂质合成、细胞增殖和细胞粘附。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-26 DOI: 10.1111/1744-7917.13438

Ke-Yan Jin, Yu-Qin Di, Tian-Wen Liu, Xiao-Fan Zhao

{"title":"Development of the insect adult fat body relies on glycolysis, lipid synthesis, cell proliferation, and cell adhesion.","authors":"Ke-Yan Jin, Yu-Qin Di, Tian-Wen Liu, Xiao-Fan Zhao","doi":"10.1111/1744-7917.13438","DOIUrl":"https://doi.org/10.1111/1744-7917.13438","url":null,"abstract":"The fat body of the holometabolous insect is remodeled by the degradation of the larval fat body and the development of the adult fat body during metamorphosis. However, the mechanism of adult fat body development is quite unclear. Using the agricultural pest Helicoverpa armigera, the cotton bollworm, as a model, we revealed that the development of adult fat body was regulated by glycolysis, triglyceride (triacylglycerol [TAG]) synthesis, cell proliferation, and cell adhesion. RNA sequencing detected a set of genes that were upregulated in the 8-d late pupal fat body at a late metamorphic stage compared with the 2-d pupal fat body at an earlier metamorphic stage. The pathways for glycolysis, TAG synthesis, cell proliferation, and cell adhesion were enriched by the differentially expressed genes, and the key genes linked with these pathways showed increased expression in the 8-d pupal fat body. Knockdown of phosphofructokinase (Pfk), acetyl-CoA carboxylase (Acc1), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit (P110) and collagen alpha-1(IV) chain (Col4a1) by RNA interference resulted in abnormal eclosion and death at pupal stages, and repressed lipid droplets accumulation and adult fat body development. The expression of Acc1, P110, and Col4a1 was repressed by the insect steroid hormone 20-hydroxyecdysone (20E). The critical genes in the 20E pathway appeared to decrease at the late pupal stage. These data suggested that the development of the insect adult fat body is regulated by glycolysis, lipids synthesis, cell proliferation, and cell adhesion at the late pupal stage when the 20E signal decreases.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensing volatiles throughout the body: geographic- and tissue-specific olfactory receptor expression in the fig wasp. 感知全身的挥发性物质：无花果蜂嗅觉受体的地理和组织特异性表达。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-25 DOI: 10.1111/1744-7917.13441

Sushma Krishnan, Snehal Dilip Karpe, Hithesh Kumar, Lucy B Nongbri, Vignesh Venkateswaran, Ramanathan Sowdhamini, Ewald Grosse-Wilde, Bill S Hansson, Renee M Borges

{"title":"Sensing volatiles throughout the body: geographic- and tissue-specific olfactory receptor expression in the fig wasp.","authors":"Sushma Krishnan, Snehal Dilip Karpe, Hithesh Kumar, Lucy B Nongbri, Vignesh Venkateswaran, Ramanathan Sowdhamini, Ewald Grosse-Wilde, Bill S Hansson, Renee M Borges","doi":"10.1111/1744-7917.13441","DOIUrl":"https://doi.org/10.1111/1744-7917.13441","url":null,"abstract":"An essential adaptive strategy in insects is the evolution of olfactory receptors (ORs) to recognize important volatile environmental chemical cues. Our model species, Ceratosolen fusciceps, a specialist wasp pollinator of Ficus racemosa, likely possesses an OR repertoire that allows it to distinguish fig-specific volatiles in highly variable environments. Using a newly assembled genome-guided transcriptome, we annotated 63 ORs in the species and reconstructed the phylogeny of Ceratosolen ORs in conjunction with other hymenopteran species. Expression analysis showed that though ORs were mainly expressed in the female antennae, 20% were also expressed in nonantennal tissues such as the head, thorax, abdomen, legs, wings, and ovipositor. Specific upregulated expression was observed in OR30C in the head and OR60C in the wings. We identified OR expression from all major body parts of female C. fusciceps, suggesting novel roles of ORs throughout the body. Further examination of the OR expression of C. fusciceps in widely separated geographical locations, that is, South (urban) and Northeast (rural) India, revealed distinct OR expression levels in different locations. This discrepancy likely parallels the observed variation in fig volatiles between these regions and provides new insights into the evolution of insect ORs and their expression across geographical locations and tissues.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entomopathogenic Bacillus cereus impairs the fitness of the spotted-wing drosophila, Drosophila suzukii. 昆虫病原蜡样芽孢杆菌损害斑翅果蝇的适应性

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-21 DOI: 10.1111/1744-7917.13439

Nana He, Shaojie Zhou, Chuanming Zhou, Weikang Yang, Sheng Zhang, Deqiang Yan, Xiaowen Ji, Wei Liu

{"title":"Entomopathogenic Bacillus cereus impairs the fitness of the spotted-wing drosophila, Drosophila suzukii.","authors":"Nana He, Shaojie Zhou, Chuanming Zhou, Weikang Yang, Sheng Zhang, Deqiang Yan, Xiaowen Ji, Wei Liu","doi":"10.1111/1744-7917.13439","DOIUrl":"https://doi.org/10.1111/1744-7917.13439","url":null,"abstract":"Drosophila suzukii is a notorious pest which causes devastating damage to thin-skinned fruits, and the larvae feed on the fruit, causing extensive agricultural economic loss. The current application of insecticides to manage this pest results in serious resistance and environmental hazards, so an alternative strategy for D. suzukii biocontrol is urgently needed. Here, we reported that entomopathogenic Bacillus cereus has the potential to biocontrol D. suzukii. We isolated and identified the bacterial strain, B. cereus H1, that was detrimental to the fitness of both D. suzukii progenies and parents. D. suzukii was robustly repelled to depositing eggs on the halves with metabolites of B. cereus H1. Both males and females of D. suzukii were susceptible to B. cereus H1. B. cereus H1 significantly arrested larval development with at least 40% lethal larvae. The median lethal time (LT50) of males and females of D. suzukii challenged with B. cereus H1 was 3 and 2 d, respectively. Moreover, B. cereus H1 disrupted the intestinal integrity and pH value of D. suzukii and resulted in an increase in bacterial load of guts and hemolymph. Mechanistically, infection of B. cereus H1 led to the activation of the dual oxidase (DUOX)-ROS-Jun N-terminal kinase (JNK) pathway. The findings showed that the entomopathogen B. cereus H1 could potentially act as a biological control agent against D. suzukii, advancing fundamental concepts of integrated pest management programs against D. suzukii.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0