Insect Science最新文献_第5页

Tissue-specific alternative splicing and the functional differentiation of LmLPMO15-1 in Locusta migratoria. 组织特异性替代剪接与蝗虫中 LmLPMO15-1 的功能分化。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-11-14 DOI: 10.1111/1744-7917.13469

Lin Kong, Huiying Hu, Pengfei Li, Mingbo Qu

{"title":"Tissue-specific alternative splicing and the functional differentiation of LmLPMO15-1 in Locusta migratoria.","authors":"Lin Kong, Huiying Hu, Pengfei Li, Mingbo Qu","doi":"10.1111/1744-7917.13469","DOIUrl":"10.1111/1744-7917.13469","url":null,"abstract":"Insect lytic polysaccharide monooxygenases (LPMO15s) are newly discovered copper-dependent enzymes that promote chitin degradation in insect through oxidative cleavage of glycosidic bonds. They are potential pesticide targets due to their critical role for chitin turnover in the integument, trachea, and peritrophic matrix of the midgut during insect molting. However, the knowledge about whether and how LPMO15s participate in chitin turnover in other tissues is still insufficient. Here, using the orthopteran pest Locusta migratoria as a model, a novel alternative splicing site of LmLPMO15-1 was discovered and it produces 2 variants, LmLPMO15-1a and LmLPMO15-1b. The transcripts of LmLPMO15-1a and LmLPMO15-1b were specifically expressed in the trachea and foregut, respectively. RNA interference targeting LmLPMO15-1 (a common fragment shared by both LmLPMO15-1a and LmLPMO15-1b), a specific region of LmLPMO15-1a or LmLPMO15-1b all significantly reduced survival rate of nymphs and induced lethal phenotypes with developmental stasis or molt failure. Ultrastructure analysis demonstrated that LmLPMO15-1b was specifically involved in foregut old cuticle degradation, while LmLPMO15-1a was exclusively responsible for the degradation of the tracheal old cuticle. This study revealed LmLPMO15-1 achieved tissue-specific functional differentiation through alternative splicing, and proved the significance of the spliced variants during insect growth and development. It provides new strategies for pest control targeting LPMO15-1.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1241-1255"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-targets cleavage of BmNPV genome through genome-wide repeat sequence using CRISPR/Cas9 antiviral system. 利用 CRISPR/Cas9 抗病毒系统通过全基因组重复序列对 BmNPV 基因组进行多靶点切割。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-20 DOI: 10.1111/1744-7917.13462

Yujia Liu, Xu Yang, Ping Wu, Xijie Guo, Zulian Liu, Yongping Huang, Xia Xu

{"title":"Multi-targets cleavage of BmNPV genome through genome-wide repeat sequence using CRISPR/Cas9 antiviral system.","authors":"Yujia Liu, Xu Yang, Ping Wu, Xijie Guo, Zulian Liu, Yongping Huang, Xia Xu","doi":"10.1111/1744-7917.13462","DOIUrl":"10.1111/1744-7917.13462","url":null,"abstract":"The escalating severity of Bombyx mori nuclear polyhedrosis virus (BmNPV) infections poses significant challenges to the silkworm industry, especially when massive production shifts occur from the eastern regions to western regions with lower labor costs. Education and experience levels are different and disease control is badly needed. To solve the problems, we have developed an innovative CRISPR/Cas9 system specifically targeting BmNPV to enhance viral resistance. For the system, we selected BmNPV genes linked to virus replication and proliferation as targets, designing 2 sites for each gene. Mutating the target sequence renders the system incapable of efficiently cleaving the virus genome, hence decreasing cleavage efficiency. We conducted a search for \"NGG\" or \"CCN\" target sequences in the BmNPV genome, excluding non-recurring and potential targets in the B. mori genome. We successfully identified 2 distinct target sequences in the BmNPV genome-one being repeated 12 times and the other three times. These sequences lead to fragmentation of virus genome into multiple large segments that are difficult to repair. Transgenic silkworms demonstrate robust resistance to viruses, significantly boosting their survival rates compared with wild-type silkworms under various virus infection concentrations. Our system efficiently targets dozens of viral genomes with just 2 sequences, minimizing transposable elements while ensuring cutting effectiveness. This marks a pioneering advancement by using repetitive elements within the virus genome for targeted CRISPR cleavage, aiming for antiviral effects through genome fragmentation rather than disrupting essential viral genes. Our research introduces innovative concepts to CRISPR antiviral investigations and shows promise for the practical application of gene editing in industrial silkworm strains.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1174-1184"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miRNA-mRNA modules enhance juvenile hormone biosynthesis for insect vitellogenesis and egg production. miRNA-mRNA 模块增强了昆虫卵黄发生和产卵过程中幼年激素的生物合成。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-30 DOI: 10.1111/1744-7917.13451

Wanwan Li, Mingzhi Liu, Zitong Zhuang, Lulu Gao, Jiasheng Song, Shutang Zhou

{"title":"The miRNA-mRNA modules enhance juvenile hormone biosynthesis for insect vitellogenesis and egg production.","authors":"Wanwan Li, Mingzhi Liu, Zitong Zhuang, Lulu Gao, Jiasheng Song, Shutang Zhou","doi":"10.1111/1744-7917.13451","DOIUrl":"10.1111/1744-7917.13451","url":null,"abstract":"In addition to preventing precocious larval metamorphosis, juvenile hormone (JH), synthesized in corpora allata (CA), is known to stimulate female reproduction of insects. JH titer is extremely low or absent during metamorphosis, but thereafter rapidly increases in the previtellogenic stage and rises to a peak in the vitellogenic phase. However, the mechanisms underlying the biosynthesis of high levels of JH in adults remain unclear. We found in this study that 12 genes involved in JH synthesis pathway were highly expressed in the CA of adult locusts. By transcriptome analysis and quantitative real-time - polymerase chain reaction validation, a total of 106 evolutionary conserved micro RNAs (miRNAs) and 163 species-specific miRNAs were identified in locust CA. Dual-luciferase assay revealed that 17 miRNAs bound to 10 JH synthesis genes (JHSGs) and downregulated their expression. These miRNAs were expressed in low levels during vitellogenic stage, which was oppositive from that of targeting JHSGs. Six miRNAs including miR-971-3p, miR-31a, miR-9-5p, miR-1-3p, miR-315, and miR-282 were selected for function study. Co-application of agomiRs resulted in significantly decreased levels of targeting JHSGs, accompanied by significantly reduced vitellogenin expression as well as arrested ovarian development. The data suggest that multiple miRNAs expressed synchronously at low levels in the vitellogenic phase, thereby ensuring the high levels of JHSG expression to facilitate JH biosynthesis required for JH-dependent female reproduction. The findings provide important information for deciphering miRNA-messenger RNA modules for JH biosynthesis as well as JH regulation of insect metamorphosis and reproduction.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1227-1240"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gallic acid enhances honeybee larvae resistance to Paenibacillus larvae infections: Insights from molecular docking, bacterial community modulation, and whole-genome sequencing. 没食子酸增强蜜蜂幼虫对芽孢杆菌幼虫感染的抵抗力：来自分子对接、细菌群落调节和全基因组测序的见解。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2025-02-25 DOI: 10.1111/1744-7917.70000

Man-Hong Ye, Yin-Hong Jiang, Xiao-Yuan Li, Qian-Nan Han, Chuang Meng, Feng Ji, Bin Zhou

{"title":"Gallic acid enhances honeybee larvae resistance to Paenibacillus larvae infections: Insights from molecular docking, bacterial community modulation, and whole-genome sequencing.","authors":"Man-Hong Ye, Yin-Hong Jiang, Xiao-Yuan Li, Qian-Nan Han, Chuang Meng, Feng Ji, Bin Zhou","doi":"10.1111/1744-7917.70000","DOIUrl":"10.1111/1744-7917.70000","url":null,"abstract":"American foulbrood (AFB) disease, caused by the bacterium Paenibacillus larvae, is a devastating disease affecting honeybee (Apis mellifera L.) populations worldwide. Commonly treated with antibiotics, which have negative impacts on both honeybees and the environment, there is an urgent need for alternatives in AFB control. This study aimed to investigate the effects of gallic acid (GA) on honeybee larvae challenged with P. larvae spores and explore its modulation of larval microbiota. Our results demonstrated that in the presence of P. larvae spores, coadministration of 125 µg/mL GA significantly increased the survival rate and body weight of honeybee larvae. Molecular docking analyses revealed that GA competitively binds to spore germination proteins YndE and GerM, with affinities comparable to L-tyrosine and stronger than uric acid, respectively, suggesting interference with P. larvae spore germination. 16S rRNA gene amplicon sequencing revealed that GA treatment augmented bacterial diversity and enriched lactic acid bacteria (LAB) in honeybee larvae. Whole-genome sequencing of 2 LAB strains, Apilactobacillus kunkeei GL-2 and Enterococcus faecium GL-6, isolated from GA-treated larvae, unveiled their potential to produce antimicrobial secondary metabolites and bacteriocins, which may contribute to their competitive advantages against P. larvae. Notably, the E. faecium GL-6 strain possessed genes encoding gallate decarboxylase, enabling GA utilization, and 2 putative bacteriocinogenic genetic clusters for enterolysin A and enterocin L50 a/b. These findings suggest that GA and the GL-6 strain hold potential as preventive measures against AFB disease in honeybees through modulation of gut microbiota and competitive inhibition of P. larvae.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1380-1402"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotonin suppresses intraspecific aggression in an agrobiont spider, Pardosa pseudoannulata, without affecting predation on insects. 羟色胺能抑制农蛛（Pardosa pseudoannulata）的种内攻击行为，但不影响对昆虫的捕食。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-09 DOI: 10.1111/1744-7917.13456

Shuchen Dong, Tao Chen, Yunru Chen, Yilin Wang, Yihao Yan, Xuerui Liu, Zewen Liu, Na Yu

{"title":"Serotonin suppresses intraspecific aggression in an agrobiont spider, Pardosa pseudoannulata, without affecting predation on insects.","authors":"Shuchen Dong, Tao Chen, Yunru Chen, Yilin Wang, Yihao Yan, Xuerui Liu, Zewen Liu, Na Yu","doi":"10.1111/1744-7917.13456","DOIUrl":"10.1111/1744-7917.13456","url":null,"abstract":"Spiders are an abundant group of natural enemies preying on insect pests in agroecosystem. But their potential in biological control has not been fully realized due to difficult mass production. One hindrance is the intense intraspecific aggression in spiders. Neurotransmitters such as serotonin play important roles in modulating aggression. Here, we investigated the regulatory function of serotonin (5-hydroxytryptamine [5-HT]) signaling in the intraspecific aggression in a wandering spider Pardosa pseudoannulata (Araneae, Lycosidae). The aggression was quantified with 5 escalated aggression behaviors as approach, chasing, lunging, boxing, and biting. Virgin (VG) females exhibited higher aggression levels but less 5-HT content than post-reproductive (PR) females. Systemic increase of 5-HT via 5-HT injection decreased aggression, while decrease of 5-HT via RNA interference (RNAi) of the tryptophan hydroxylase gene, increased aggression. The involvement of the four 5-HT receptors were determined via individual or combined RNAi. Co-RNAi of the three 5-HT1 genes increased overall aggression with decreased incidents of approach, chasing, lunging, and increased biting. RNAi of 5-HT1B decreased approach and increased biting, whereas RNAi of 5-HT1A or 5-HT1C did not affect aggression. RNAi of 5-HT7 decreased approach only. Therefore, different 5-HT receptor types contribute to different aspects of the inhibitory effects of 5-HT on aggression and provide several pharmacological targets for manipulating spider aggression. 5-HT injection did not affect spiders' predation on their insect prey, the brown planthopper Nilaparvata lugens. The findings reveal 1 neuronal mechanism regulating intraspecific aggression in spiders and provide an insight in developing aggression suppression strategies for spider mass rearing.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1427-1438"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori. BmEL-2通过调控BmAGPATγ和BmFAF2的表达来促进桑蚕体内甘油三酯的代谢。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-15 DOI: 10.1111/1744-7917.13446

Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun

{"title":"BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori.","authors":"Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun","doi":"10.1111/1744-7917.13446","DOIUrl":"10.1111/1744-7917.13446","url":null,"abstract":"Bombyx mori ELAV-like-1 (BmEL-1) and B. mori ELAV-like-2 (BmEL-2) are 2 members of the ELAV-like family of RNA-binding proteins. Mutations in Bmel-1 and Bmel-2 resulted in 5.8% and 28.5% decreases in larval weight on the 3rd day of the 5th instar larva (L5D3), respectively. Triglycerides (TG) are the most important energy resource and are the main component of neutral fat (NF) in animals. To investigate the role of Bmelav-like genes in the synthesis and decomposition of TG, transcriptomic, and metabolic analyses were performed on the whole bodies on the 1st day of the 2nd instar larvae (L2D1) and on fat bodies on L5D3 of Bmel-1- and Bmel-2- mutants, respectively. As compared with the control silkworm, differentially expressed genes generated in both mutants were mainly enriched in lysine degradation, fatty acid (FA) metabolism, and unsaturated FAs biosynthesis. The diglyceride and phosphatide contents were significantly lower in Bmel-1- and Bmel-2- fat bodies than those of the control group. Consistently, the NF content of both mutants' fat bodies were reduced by 50% and 60%, respectively. BmEL-2 positively regulates BmAGPATγ (B. mori 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, LOC101741736) and BmFaF2 (B. mori fatty acid synthetase-associated factor 2, LOC101739090) expression by binding to the specific regions of their 3' untranslated regions in BmN cells. This study suggests that BmEL-2 may be an important regulator of BmAGPATγ and BmFAF2 expression and thereby participates in TG metabolism in the silkworm fat body.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1256-1268"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diet acts on sexual behavior development in a male moth. 饮食对雄蛾性行为发展的影响

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-14 DOI: 10.1111/1744-7917.13457

Evan Force, Caroline Suray, Christelle Monsempes, Chloé Danis, Gabrielle Bonfils, Stéphane Debernard, Matthieu Dacher

{"title":"Diet acts on sexual behavior development in a male moth.","authors":"Evan Force, Caroline Suray, Christelle Monsempes, Chloé Danis, Gabrielle Bonfils, Stéphane Debernard, Matthieu Dacher","doi":"10.1111/1744-7917.13457","DOIUrl":"10.1111/1744-7917.13457","url":null,"abstract":"In many animals, drastic changes are observed during sexual maturation characterized by the reproductive system development concomitantly to the sexual behavior ontogenesis. These modifications are under the control of internal and external factors such as food. Sexual maturation requires considerable energetic investment, and diet has been shown to affect reproductive activities in many taxonomic groups, especially in insects and vertebrates. By contrast, diet effects on sexual behavior development remain largely unexplored. To elucidate this aspect, we used the male moth Agrotis ipsilon which undergoes sexual maturation occurring between the third and the fifth day postemergence. During this period, males are sensitive to female sex pheromones and a stereotypical sexual behavior characterized by female-oriented flight takes place. In our study, we compared (1) sex pheromone detection by electroantennography recordings and (2) behavioral response in wind tunnel assays between males fed with different diets found in nature. Compared to standard sucrose diet, males fed with sucrose, fructose, and glucose supplemented with sodium (a mineral element necessary for the locomotor activity in several moths) did not respond better to female sex pheromones but clearly exhibited an earlier behavioral response. Thus, such a diet accelerates the development of sex pheromone-mediated oriented flight, probably by facilitating the central processing of sex pheromone information in male A. ipsilon moths. Our results provide new information on the influence of nutritional intake on the ontogenesis of male sexual behavior in animals.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1439-1452"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deterrence and behavioral mode of coconut oil-derived free fatty acids on Zeugodacus cucurbitae oviposition. 椰子油游离脂肪酸对葫芦蝇产卵的威慑和行为模式

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-11-03 DOI: 10.1111/1744-7917.13460

Vijaya Movva, Junwei Zhu, Amy Roda, Paul Kendra, Xiangbing Yang, Kevin Cloonan, Jia-Wei Tay, Dong H Cha

{"title":"Deterrence and behavioral mode of coconut oil-derived free fatty acids on Zeugodacus cucurbitae oviposition.","authors":"Vijaya Movva, Junwei Zhu, Amy Roda, Paul Kendra, Xiangbing Yang, Kevin Cloonan, Jia-Wei Tay, Dong H Cha","doi":"10.1111/1744-7917.13460","DOIUrl":"10.1111/1744-7917.13460","url":null,"abstract":"Previous studies have shown oviposition deterring properties of 8 coconut free fatty acid (CFFA) compounds on fruit flies with different key deterrent components for different species. Here we evaluated oviposition deterrence of CFFA using laboratory 2-choice bioassays against Zeugodacus cucurbitae, determined key-bioactive deterrent compounds, and evaluated their behavioral mode. Unlike other reported fruit fly species, CFFA mixture increased Z. cucurbitae oviposition when directly applied on an oviposition substrate. When tested individually in subsequent tests, 4 compounds (caprylic, capric, oleic, and linoleic acids) significantly reduced the oviposition (\"negative-compounds\"), 1 compound (stearic acid) had no effect (\"neutral-compound\"), and 3 compounds (lauric, myristic, and palmitic acids) stimulated the oviposition (\"positive-compounds\"). The 4-component negative-compound blend was effective at reducing oviposition. However, adding stearic acid to the 4-component blend (5-component blend, 5c) further reduced oviposition. Adding any of the positive-compounds to the 5c resulted in loss of oviposition deterrence, suggesting the 5c as the key deterrent component blend. The blend was also effective in no-choice assays and when applied on cucumbers, a preferred host of Z. cucurbitae. When given a choice, Z. cucurbitae made 48.5% fewer visits, spent 39% less time, and oviposited 88.2% fewer eggs per min on 5c treated pumpkin agar than on control agar, suggesting that the 5c blend has both spatial repellency and contact deterrence. Given that all compounds are registered food additives and generally regarded as safe, this blend has potential application in behavioral control strategies, such as push-pull, to protect host fruit against Z. cucurbitae.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1403-1414"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific variation of thermal tolerance along elevational gradients: the case of a widespread diving beetle (Coleoptera: Dytiscidae). 热耐受性随海拔梯度的种内变异：一种广泛分布的潜水甲虫（鞘翅目：Dytiscidae）的案例。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-11-25 DOI: 10.1111/1744-7917.13466

Susana Pallarés, José Antonio Carbonell, Félix Picazo, David T Bilton, Andrés Millán, Pedro Abellán

{"title":"Intraspecific variation of thermal tolerance along elevational gradients: the case of a widespread diving beetle (Coleoptera: Dytiscidae).","authors":"Susana Pallarés, José Antonio Carbonell, Félix Picazo, David T Bilton, Andrés Millán, Pedro Abellán","doi":"10.1111/1744-7917.13466","DOIUrl":"10.1111/1744-7917.13466","url":null,"abstract":"Species distributed across wide elevational gradients are likely to experience local thermal adaptation and exhibit high thermal plasticity, as these gradients are characterised by steep environmental changes over short geographic distances (i.e., strong selection differentials). The prevalence of adaptive intraspecific variation in thermal tolerance with elevation remains unclear, however, particularly in freshwater taxa. We explored variation in upper and lower thermal limits and acclimation capacity among Iberian populations of adults of the widespread water beetle Agabus bipustulatus (Dytiscidae) across a 2000 m elevational gradient, from lowland to alpine areas. Since mean and extreme temperatures decline with elevation, we predicted that populations at higher elevations will show lower heat tolerance and higher cold tolerance than lowland ones. We also explored whether acclimation capacity is positively related with climatic variability across elevations. We found significant variation in thermal limits between populations of A. bipustulatus, but no evidence of local adaptation to different thermal conditions across the altitudinal gradient, as relationships between thermal limits and elevation or climatic variables were largely nonsignificant. Furthermore, plasticities of both upper and lower thermal limits were consistently low in all populations. These results suggest thermal niche conservatism in this species, likely due to gene flow counteracting the effects of divergent selection, or adaptations in other traits that buffer exposure to climate extremes. The limited adaptive potential and plasticity of thermal tolerance observed in A. bipustulatus suggest that even generalist species, distributed across wide environmental gradients, may have limited resilience to global warming.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1453-1465"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative techniques for insect behavior analysis using micro-CT and Blender. 利用微型 CT 和 Blender 进行昆虫行为分析的综合技术。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-16 DOI: 10.1111/1744-7917.13458

Lei Jiang, Wenjie Li, Xiaokun Liu, Congqiao Li, Zonghui Sun, Fengming Wu, Siqin Ge

引用次数: 0