Insect Science最新文献_第5页

The role of pig manure-derived gut microbiota in accelerating the growth and development of Sarcophaga africa. 猪粪便来源的肠道微生物群在加速非洲石棺菌生长和发育中的作用。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-06-30 DOI: 10.1111/1744-7917.70104

Fengqin Yang, Xiangyan Zhang, Yanjie Shang, Jiao Xiao, Jian Zhao, Xingchun Zhao, Yadong Guo

{"title":"The role of pig manure-derived gut microbiota in accelerating the growth and development of Sarcophaga africa.","authors":"Fengqin Yang, Xiangyan Zhang, Yanjie Shang, Jiao Xiao, Jian Zhao, Xingchun Zhao, Yadong Guo","doi":"10.1111/1744-7917.70104","DOIUrl":"10.1111/1744-7917.70104","url":null,"abstract":"Exploring the impact of diet-derived gut microbiota on insect growth and development holds significant value not only for understanding the \"diet-gut microbes-insects\" interactions, but also for forensic entomology, as it lays the research foundation for clarifying the relationships among \"cadavers-microbes-necrophagous insects\". This study investigates the effects of pig manure-derived gut microbiota on the growth and development of Sarcophaga africa, a key necrophagous insect. Our findings demonstrate that, compared to pig lung, pig manure significantly increased larval amount without affecting adult eclosion rates, thereby facilitating laboratory population establishment and accelerating development. Through microbial sequencing, key target bacteria were identified and validated using quantitative detection and antibiotic interference experiments. The results highlight two critical aspects: first, dietary microbes play a central role in shaping the gut microbial community structure of larvae; second, gut microbes derived from pig manure, particularly Clostridium_sensu_stricto_1, may enhance the developmental process of S. africa by directly increasing protein levels in 3rd instar larvae and indirectly reducing glycogen levels in late pupae. Additionally, developmental data for S. africa at 30 °C were obtained to establish reference values for forensic applications using necrophagous insects in postmortem interval estimation. These findings highlight the need for forensic entomologists to consider how microbial-driven developmental variations may affect the reliability of developmental data in such studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2025-2039"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maltase 1 regulates DENV2 infection and life history in Aedes aegypti. 马耳他酶1调控埃及伊蚊DENV2感染和生活史。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-22 DOI: 10.1111/1744-7917.70122

Man-Jin Li, Jin Cheng, Ya-Ming Zou, Quan-Chao Liu, Ding Zhu, Ce-Jie Lan

引用次数: 0

Bumblebees prefer shorter over longer strings and connected over disconnected ones in string-pulling tasks but prioritize connectivity over spatial proximity when the two are in conflict. 在拉绳任务中，大黄蜂更喜欢短的绳子而不是长绳子，更喜欢连接的绳子而不是断开的绳子，但当两者冲突时，它们会优先考虑连通性而不是空间接近性。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-02 DOI: 10.1111/1744-7917.70113

Chao Wen, Shunping Dong, Yuhao Guo, Cai Wang, Weicheng Ding, Jin Ge, Junbao Wen, Lars Chittka

{"title":"Bumblebees prefer shorter over longer strings and connected over disconnected ones in string-pulling tasks but prioritize connectivity over spatial proximity when the two are in conflict.","authors":"Chao Wen, Shunping Dong, Yuhao Guo, Cai Wang, Weicheng Ding, Jin Ge, Junbao Wen, Lars Chittka","doi":"10.1111/1744-7917.70113","DOIUrl":"10.1111/1744-7917.70113","url":null,"abstract":"We aimed to investigate the trade-off strategies between efficiency and accessibility employed by bumblebees (Bombus terrestris) when solving string-pulling tasks. In these tasks, bees were required to choose between two strings, which varied in length, and whether or not they were connected to an artificial flower. Our results indicate that when presented with strings of varying lengths connected to artificial flowers containing rewards, the bees consistently preferred the shorter string. Instead of learning the string length experienced during training, the bees showed a preference for the shorter string, even after being trained with a longer one. Bumblebees were also capable of selecting connected over disconnected strings, even when proximity served as a confounding factor. These findings demonstrate that bumblebees flexibly deploy a diversity of strategies to solve string-pulling tasks, shedding light on their problem-solving capabilities when tackling non-natural spatial and object manipulation tasks.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2097-2108"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuticular hydrocarbon trafficking in insects: Transport, deposition, and barrier assembly. 昆虫表皮的碳氢化合物运输：运输、沉积和屏障组装。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-30 DOI: 10.1111/1744-7917.70374

Jian-Wen Qiao, Syed Husne Mobarak, Li Li, Bing-Jin Wu, Tong-Xian Liu

{"title":"Cuticular hydrocarbon trafficking in insects: Transport, deposition, and barrier assembly.","authors":"Jian-Wen Qiao, Syed Husne Mobarak, Li Li, Bing-Jin Wu, Tong-Xian Liu","doi":"10.1111/1744-7917.70374","DOIUrl":"https://doi.org/10.1111/1744-7917.70374","url":null,"abstract":"Cuticular hydrocarbons (CHCs) contribute to water retention, cuticle barrier integrity, protection against xenobiotic penetration, and chemical communication in insects. Classical physiological and biochemical studies established that hydrocarbons are synthesized mainly in oenocytes, transported through the hemolymph by lipophorin, and, in several insect systems, selectively accumulated at the cuticle and other target tissues. Building on this framework, recent genetic, cellular, and structural studies have begun to characterize molecular components associated with tissue accumulation, epidermal export, and pore-canal organization, including lipophorin receptors, ATP-binding cassette transporters, and extracellular pore-canal factors. However, the evidence remains limited, taxonomically concentrated, and derived largely from a small number of experimental systems, while many reported phenotypes do not distinguish direct hydrocarbon transport from broader effects on lipid allocation, membrane organization, or cuticle development. This review integrates classical physiological evidence with recent molecular studies of hydrocarbon production, systemic carriage, tissue accumulation, epidermal export, and surface deposition. It evaluates the comparative distribution and evidential strength of current findings and identifies unresolved questions concerning hydrocarbon loading and unloading, substrate specificity, extracellular passage through pore canals, and regional deposition. By separating established processes from functional associations and untested hypotheses, this synthesis clarifies the present limits of the field and defines the evidence required to determine how chemically distinct hydrocarbons reach specific functional destinations.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lmx1b is essential for molting and leg integrity in Henosepilachna vigintioctopunctata: Implications for RNA biopesticide development. Lmx1b对Henosepilachna viintioctopunctata的脱皮和腿部完整性至关重要：对RNA生物农药开发的影响。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-30 DOI: 10.1111/1744-7917.70372

Junna Liu, Jing He, Mujuan Guo, Ran Gao, June-Sun Yoon, Xuguo Zhou, Youjun Zhang, Huipeng Pan, Chunxiao Yang

{"title":"Lmx1b is essential for molting and leg integrity in Henosepilachna vigintioctopunctata: Implications for RNA biopesticide development.","authors":"Junna Liu, Jing He, Mujuan Guo, Ran Gao, June-Sun Yoon, Xuguo Zhou, Youjun Zhang, Huipeng Pan, Chunxiao Yang","doi":"10.1111/1744-7917.70372","DOIUrl":"https://doi.org/10.1111/1744-7917.70372","url":null,"abstract":"LIM homeodomain transcription factor 1 beta (Lmx1b), a member of the LIM-homeodomain family, plays essential roles in tissue differentiation and organ development in diverse eukaryotes, yet its biological functions in insects remain largely unknown. Here, we identify Lmx1b as a highly effective RNA interference (RNAi) target in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata, a serious solanaceous pest. RNAi-mediated silencing of HvLmx1b prevented larval molting and pupation, resulting in complete mortality. HvLmx1b knockdown markedly reduced endogenous 20-hydroxyecdysone (20E) titers, suppressed Halloween genes and the ecdysone signaling pathway, and downregulated multiple genes involved in chitin metabolism and cuticle tanning, resulting in defective cuticle formation. Ultrastructural analyses further revealed progressive degeneration of epidermal cells and leg muscles, indicating an essential role for HvLmx1b in maintaining tissue integrity during postembryonic development. In silico off-target analysis against representative non-target arthropods and beneficial organisms predicted a low risk of unintended gene silencing by dsHvLmx1b. Collectively, our findings establish HvLmx1b as an upstream developmental regulator that coordinates ecdysteroid signaling, cuticle formation and postembryonic tissue homeostasis, and highlight its potential as an RNAi target for management of H. vigintioctopunctata.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting DOPA decarboxylase disrupts immune-metabolic coordination and renders Spodoptera frugiperda susceptible to bacterial infection. 靶向多巴脱羧酶破坏免疫代谢协调，使夜蛾易受细菌感染。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-28 DOI: 10.1111/1744-7917.70370

Ze-Yuan Zhang, Shang-Huan Huang, Shen-Lei Li, Jin-Fang Tan, Zhao-Wei Wang, Yuan Zou, Guan-Heng Zhu

{"title":"Targeting DOPA decarboxylase disrupts immune-metabolic coordination and renders Spodoptera frugiperda susceptible to bacterial infection.","authors":"Ze-Yuan Zhang, Shang-Huan Huang, Shen-Lei Li, Jin-Fang Tan, Zhao-Wei Wang, Yuan Zou, Guan-Heng Zhu","doi":"10.1111/1744-7917.70370","DOIUrl":"https://doi.org/10.1111/1744-7917.70370","url":null,"abstract":"DOPA decarboxylase (DDC) is a key enzyme that connects melanin production and innate immunity in insects, yet its regulatory network in major lepidopteran pests remains poorly understood. We combined CRISPR/Cas9 gene editing with multi-omics analysis to explore DDC's role in immune responses in the invasive pest Spodoptera frugiperda. Knocking out DDC caused complete albinism, severe developmental delays, pronounced developmental defects, and increased susceptibility to bacterial challenge. Following the E. coli challenge, DDC-mutant larvae showed substantially lower final survival than infected wild-type larvae (6.7% vs. 66.7%) and failed to pupate, despite pronounced cuticular darkening. Transcriptomic and metabolomic analyses showed that DDC loss disrupted the tyrosine-dopamine pathway and was associated with altered expression of genes related to the prophenoloxidase cascade. Notably, laccase2 was transcriptionally upregulated after challenge; however, its contribution to infection-associated cuticular darkening remains to be determined. This response did not restore dopamine-dependent immune signaling or whole-body metabolism, as evidenced by disruptions in glycine/serine pathways and reduced immune gene activity. These results separate pigmentation from immune functions within the melanin pathway, highlighting DDC as a crucial immune-metabolic hub. Given its roles in development and immunity and the high conservation of the dopamine pathway in invertebrates, DDC is a promising target for species-specific, resistance-proof RNAi pest control methods.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

iORbase 2.0: Comprehensive analysis platform for insect odorant receptors from genome to structure and function. iORbase 2.0：昆虫气味受体从基因组到结构和功能的综合分析平台。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-28 DOI: 10.1111/1744-7917.70375

Yifeng Zhang, Chenxi Gao, Jiameng Xue, Yi Guo, Suyang Duan, Tianmin Zhang, Wei Xue, Xiang Yao, Yangming Yin, Qingbin Sun, Gang Li, Chang Xu, Huimeng Lu

引用次数: 0

Mechanisms of insect resistance to RNA-based biopesticides and management strategies. 昆虫对rna基生物农药的抗性机制及管理策略。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-27 DOI: 10.1111/1744-7917.70371

Xiang Li, Liu-Yi Fan, Kai-Bo Wang, Ruo-Bing Guan

{"title":"Mechanisms of insect resistance to RNA-based biopesticides and management strategies.","authors":"Xiang Li, Liu-Yi Fan, Kai-Bo Wang, Ruo-Bing Guan","doi":"10.1111/1744-7917.70371","DOIUrl":"https://doi.org/10.1111/1744-7917.70371","url":null,"abstract":"Growing global concern about the environmental risks posed by chemical insecticides has positioned RNA interference (RNAi)-based biopesticides at the forefront of research in green agricultural pest management, due to their precise target specificity and environmental safety. With ongoing technological advances and expanding use, the emergence of insect resistance to this technology has become a major constraint on their sustainable application. This paper provides a systematic review of delivery strategies and current applications of RNA-based biopesticides in pest control, and examines the mechanisms underlying insect resistance, including species-specific sensitivity variations, disrupted dsRNA uptake, and resistance influenced by viruses or symbionts. To address the risk of resistance development, the study outlines integrated management strategies such as pesticide rotation and mixtures, optimized dosage delivery, refuge implementation, and innovative delivery technologies. Looking ahead, continued technological innovation, strengthened integrated pest management frameworks, and the creation of a global resistance monitoring network will be essential to support the sustainable use of this technology, thereby advancing the green transformation of agriculture and ensuring global food security.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lepidopteran pests and phytopathogenic fungi: The overlooked interface of insect‒microbe interactions. 鳞翅目害虫和植物病原真菌：昆虫-微生物相互作用的被忽视的界面。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-25 DOI: 10.1111/1744-7917.70352

Wen-Xue Zhang, Hao-Ran Li, Xue Guo, Wei Zhang

{"title":"Lepidopteran pests and phytopathogenic fungi: The overlooked interface of insect‒microbe interactions.","authors":"Wen-Xue Zhang, Hao-Ran Li, Xue Guo, Wei Zhang","doi":"10.1111/1744-7917.70352","DOIUrl":"https://doi.org/10.1111/1744-7917.70352","url":null,"abstract":"Lepidopteran pests and phytopathogenic fungi are major biotic threats to crop health and productivity, yet their interactions remain poorly integrated into current frameworks of insect-microbe and plant disease ecology. Lepidopteran pests and fungal pathogens often share common ecological niches on host plants, particularly damaged tissues and microbe-rich interfaces. Chewing damage, frass deposition and movement by lepidopteran pests can create opportunities for fungal dispersal and invasion. This review synthesizes current knowledge on lepidopteran pest-phytopathogenic fungus-plant (LPP) tripartite interactions. It first outlines the biological features of lepidopteran pests, phytopathogenic fungi, and host plants that provide the ecological basis for their co-occurrence. Direct interactions are then examined, including competition for plant-derived resources, pest-mediated fungal facilitation, mutualistic associations, and antagonistic effects of fungi on lepidopteran survival or development. Plant-mediated indirect interactions are further discussed, focusing on how pest feeding or fungal infection reshapes host-plant physiology, defense responses, and ecological signaling. Finally, interaction-guided strategies are considered from three perspectives: breeding crop varieties with coordinated resistance, applying RNAi-based approaches to target key pest or fungal genes, and manipulating plant- or insect-associated microbial communities. Overall, understanding the pest-fungus interactions shifts the paradigm from isolated threat management toward holistic interventions, providing a mechanistic foundation for next-generation sustainable crop protection.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149060755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-action MINPP1 effectors enable Riptortus pedestris to overcome phytic acid antinutritional defense and suppress pattern-triggered immunity. 双作用MINPP1效应使Riptortus行人克服植酸抗营养防御和抑制模式触发的免疫。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-25 DOI: 10.1111/1744-7917.70369

Yuxia Yang, Xin Zhou, Lanping Ding, Yancong Zhang, Qingsong Zhang, Gan Ai, Zhiyuan Yin, Danyu Shen, Yumei Dong, Jixing Xia, Ai Xia

{"title":"Dual-action MINPP1 effectors enable Riptortus pedestris to overcome phytic acid antinutritional defense and suppress pattern-triggered immunity.","authors":"Yuxia Yang, Xin Zhou, Lanping Ding, Yancong Zhang, Qingsong Zhang, Gan Ai, Zhiyuan Yin, Danyu Shen, Yumei Dong, Jixing Xia, Ai Xia","doi":"10.1111/1744-7917.70369","DOIUrl":"https://doi.org/10.1111/1744-7917.70369","url":null,"abstract":"Phytic acid (IP6) serves as the primary phosphorus reservoir in plant seeds and functions as a potent antinutritional defense to inhibit herbivore growth. Despite this formidable barrier, the bean bug Riptortus pedestris preferentially feeds on IP6-rich leguminous seeds, yet the molecular mechanisms underlying this dietary adaptation have remained elusive. Here, we demonstrate that R. pedestris overcomes this nutritional defense by co-opting the ancient, highly conserved multiple inositol polyphosphate phosphatase 1 (MINPP1) family. We identified four RPMINPP1 paralogs abundantly expressed in both the salivary glands and the midgut. Using 3 1P NMR spectroscopy, we showed that these enzymes completely dephosphorylate IP6 to inorganic phosphate. Functional analyses revealed a sophisticated dual-action detoxification strategy: salivary RPMINPP1 is secreted into host tissues to degrade IP6 in planta, whereas midgut-localized RPMINPP1Ls mediate IP6 hydrolysis in vivo. RNA interference experiments confirmed that RPMINPP1Ls are essential for insect feeding and survival on soybean hosts. Remarkably, RPMINPP1 also functions as a broad-spectrum suppressor of pattern-triggered immunity (PTI) triggered by diverse elicitors, acting independently of its catalytic activity. Collectively, our findings uncover a dual-pronged strategy in which an herbivore repurposes MINPP1 family effectors to simultaneously neutralize phytic acid-based antinutritional defense and suppress host plant immunity.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149060688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0