Insect Science最新文献_第6页

Impact of long-term mass-rearing on the genetic structure of tsetse fly Glossina palpalis gambiensis colonies. 长期大规模饲养对冈比亚舌蝇种群遗传结构的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-11 DOI: 10.1111/1744-7917.13479

Kiswend-Sida M Dera, Soumaïla Pagabeleguem, Tito Tresor Melachio Tanekou, Ange Irénée Toé, Gisèle Marie Sophie Ouedraogo/Sanou, Adrien Marie Gaston Belem, Sophie Ravel, Robert L Mach, Marc J B Vreysen, Adly M M Abd-Alla

{"title":"Impact of long-term mass-rearing on the genetic structure of tsetse fly Glossina palpalis gambiensis colonies.","authors":"Kiswend-Sida M Dera, Soumaïla Pagabeleguem, Tito Tresor Melachio Tanekou, Ange Irénée Toé, Gisèle Marie Sophie Ouedraogo/Sanou, Adrien Marie Gaston Belem, Sophie Ravel, Robert L Mach, Marc J B Vreysen, Adly M M Abd-Alla","doi":"10.1111/1744-7917.13479","DOIUrl":"https://doi.org/10.1111/1744-7917.13479","url":null,"abstract":"Tsetse flies are the sole cyclic vectors of African trypanosomes, which cause human and animal African trypanosomiases in Africa. Tsetse fly control remains a promising option for disease management. The sterile insect technique (SIT) stands as an environmentally friendly tool to control tsetse populations. SIT requires the mass-rearing of competent sterile males to mate with wild females. However, long-term colonization might affect the genetic structure of the reared flies. This study investigated the genetic structure of four Glossina palpalis gambiensis colonies of different ages: two originating from Senegal (SEN and ICIRSEN) and two from Burkina Faso (CIR and IBD). Samples from these colonies were genotyped at ten microsatellite loci, followed by downstream population genetic analyses. The results show that the two colonies from Burkina Faso collected from close sites (∼20 km apart) over 45-year interval retained the same genetic background (FST_CIR∼IBD ≈ 0, P-value = 0.47). These flies were however, genetically different from those from the Senegal colonies (FST_CIR∼SEN ≈ 0.047; FST_IBD∼SEN ≈ 0.058, P-value = 10-4). Moreover, no significant difference was detected in the gene diversity of the CIR and IBD colonies, with HS values of 0.650 and 0.665, respectively. The inbreeding coefficient showed that all four colonies where under Hardy-Weinberg equilibrium, with FIS values of 0.026, 0.012, -0.064, and 0.001, for CIR, IBD, ICIRSEN, and SEN, respectively. Furthermore, no sign of a recent bottleneck was identified in tsetse samples from any of the four colonies. The results suggest that long-term mass-rearing of tsetse flies has no significant impact on their genetic background and diversity.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterization of chemosensory genes in olfactory and taste organs of Spodoptera litura (Lepidoptera: Noctuidae). 鳞翅目：夜蛾科）嗅觉和味觉器官中化学感知基因的鉴定和功能表征。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-14 DOI: 10.1111/1744-7917.13350

Jun Yang, Bao-Tong Mo, Guo-Cheng Li, Ling-Qiao Huang, Hao Guo, Chen-Zhu Wang

{"title":"Identification and functional characterization of chemosensory genes in olfactory and taste organs of Spodoptera litura (Lepidoptera: Noctuidae).","authors":"Jun Yang, Bao-Tong Mo, Guo-Cheng Li, Ling-Qiao Huang, Hao Guo, Chen-Zhu Wang","doi":"10.1111/1744-7917.13350","DOIUrl":"10.1111/1744-7917.13350","url":null,"abstract":"The tobacco cutworm Spodoptera litura is one of the most destructive polyphagous crop pests. Olfaction and taste play a crucial role in its host plant selection and sexual communication, but the expression profile of chemosensory genes remains unclear. In this study, we identified 185 chemosensory genes from 7 organs in S. litura by transcriptome sequencing, of which 72 genes were published for the first time, including 27 odorant receptors (ORs), 26 gustatory receptors (GRs), 1 ionotropic receptor (IR), 16 odorant-binding proteins (OBPs), and 2 chemosensory proteins (CSPs). Phylogenetic analyses revealed that ORs, IRs, OBPs, and sensory neuron membrane proteins (SNMPs) were mainly expressed in antennae and sequence-conserved among Noctuidae species. The most differentially expressed genes (DEGs) between sexes were ORs and OBPs, and no DEGs were found in GRs. GR transcripts were enriched in proboscis, and the expression of sugar receptors was the highest. Carbon dioxide receptors, sugar receptor-SliuGR6, and bitter GRs-SlituGR43 and SlituGR66 had higher sequence identities between Noctuidae species. CSPs were broadly expressed in various organs, and SlituCSP13 was a DEG in adult antennae. The functional analysis in the Drosophila OR67d expression system found that SlituOR50, a receptor highly expressed in female antennae, is selectively tuned to farnesyl acetate. The results provide a solid foundation for understanding the molecular mechanisms by which chemosensory genes operate to elicit behavioral responses in polyphagous insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1721-1742"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of two Bursicon genes and their association with wing development in the brown citrus aphid, Aphis citricidus. 两种 Bursicon 基因的特征及其与褐柑橘蚜（Aphis citricidus）翅膀发育的关系。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-09 DOI: 10.1111/1744-7917.13337

Jin-Ming Lu, Feng Shang, Bi-Yue Ding, Lin Wang, Qing-Chun Li, Jin-Jun Wang, Wei Dou

{"title":"Characterization of two Bursicon genes and their association with wing development in the brown citrus aphid, Aphis citricidus.","authors":"Jin-Ming Lu, Feng Shang, Bi-Yue Ding, Lin Wang, Qing-Chun Li, Jin-Jun Wang, Wei Dou","doi":"10.1111/1744-7917.13337","DOIUrl":"10.1111/1744-7917.13337","url":null,"abstract":"The tanning hormone, Bursicon, is a neuropeptide secreted by the insect nervous system that functions as a heterodimer composed of Burs-α and Burs-β subunits. It plays a critical role in the processes of cuticle tanning and wing expansion in insects. In this study, we successfully identified the AcBurs-α and AcBurs-β genes in Aphis citricidus. The open reading frames of AcBurs-α and AcBurs-β were 480 and 417 bp in length, respectively. Both AcBurs-α and AcBurs-β exhibited 11 conserved cysteine residues. AcBurs-α and AcBurs-β were expressed during all developmental stages of A. citricidus and showed high expression levels in the winged aphids. To investigate the potential role of AcBurs-α and AcBurs-β in wing development, we employed RNA interference (RNAi) techniques. With the efficient silencing of AcBurs-α (44.90%) and AcBurs-β (52.31%), malformed wings were induced in aphids. The proportions of malformed wings were 22.50%, 25.84%, and 38.34% in dsAcBurs-α-, dsAcBur-β-, and dsAcBurs-α + dsAcBur-β-treated groups, respectively. Moreover, feeding protein kinase A inhibitors (H-89) also increased the proportion of malformed wings to 30.00%. Feeding both double-stranded RNA and inhibitors (H-89) significantly downregulated the wing development-related genes nubbin, vestigial, notch and spalt major. Silence of vestigial through RNAi also led to malformed wings. Meanwhile, the exogenous application of 3 hormones that influence wing development did not affect the expression level of AcBursicon genes. These findings indicate that AcBursicon genes plays a crucial role in wing development in A. citricidus; therefore, it represents a potential molecular target for the control of this pest through RNAi-based approaches.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1684-1696"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial changes and associated metabolic responses modify host plant adaptation in Stephanitis nashi. 微生物变化和相关代谢反应改变了斯蒂芬妮丝（Stephanitis nashi）寄主植物的适应性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-18 DOI: 10.1111/1744-7917.13340

Tong-Pu Li, Chen-Hao Wang, Jia-Chu Xie, Meng-Ke Wang, Jie Chen, Yu-Xi Zhu, De-Jun Hao, Xiao-Yue Hong

{"title":"Microbial changes and associated metabolic responses modify host plant adaptation in Stephanitis nashi.","authors":"Tong-Pu Li, Chen-Hao Wang, Jia-Chu Xie, Meng-Ke Wang, Jie Chen, Yu-Xi Zhu, De-Jun Hao, Xiao-Yue Hong","doi":"10.1111/1744-7917.13340","DOIUrl":"10.1111/1744-7917.13340","url":null,"abstract":"Symbiotic microorganisms are essential for the physiological processes of herbivorous pests, including the pear lace bug Stephanitis nashi, which is known for causing extensive damage to garden plants and fruit trees due to its exceptional adaptability to diverse host plants. However, the specific functional effects of the microbiome on the adaptation of S. nashi to its host plants remains unclear. Here, we identified significant microbial changes in S. nashi on 2 different host plants, crabapple and cherry blossom, characterized by the differences in fungal diversity as well as bacterial and fungal community structures, with abundant correlations between bacteria or fungi. Consistent with the microbiome changes, S. nashi that fed on cherry blossom demonstrated decreased metabolites and downregulated key metabolic pathways, such as the arginine and mitogen-activated protein kinase signaling pathway, which were crucial for host plant adaptation. Furthermore, correlation analysis unveiled numerous correlations between differential microorganisms and differential metabolites, which were influenced by the interactions between bacteria or fungi. These differential bacteria, fungi, and associated metabolites may modify the key metabolic pathways in S. nashi, aiding its adaptation to different host plants. These results provide valuable insights into the alteration in microbiome and function of S. nashi adapted to different host plants, contributing to a better understanding of pest invasion and dispersal from a microbial perspective.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1789-1809"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rainfall during the night can trigger non-migratory take-off behavior of the white-backed planthopper, Sogatella furcifera. 夜间降雨会引发白背飞虱（Sogatella furcifera）的非迁徙性起飞行为。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-27 DOI: 10.1111/1744-7917.13347

Haibo Yang, Yunlong Feng, Pinhong Zhu, Dingxu Li, Gao Hu

{"title":"Rainfall during the night can trigger non-migratory take-off behavior of the white-backed planthopper, Sogatella furcifera.","authors":"Haibo Yang, Yunlong Feng, Pinhong Zhu, Dingxu Li, Gao Hu","doi":"10.1111/1744-7917.13347","DOIUrl":"10.1111/1744-7917.13347","url":null,"abstract":"Take-off behavior is crucial to the overall success of insect migration. Although most high-altitude migratory flights commence with mass take-offs around dusk and dawn, little is known about nighttime take-off behavior. The take-off behavior of migratory Sogatella furcifera was investigated in field cages from 2017 to 2019. The species showed a bimodal take-off pattern at dusk and dawn on rainless nights, with mass flight at dusk more intense than dawn flight. However, a higher frequency of take-offs during the nighttime was observed on rainy nights, resulting in the absence of dawn take-offs. Most migratory take-off individuals at dusk and dawn landed on the cage top or the walls above 150 cm, while non-migratory individuals that took off during the nighttime due to rainfall mainly landed on the cage walls below 150 cm. Furthermore, it has been observed that migratory take-off individuals possess stronger sustained flight capabilities and exhibit more immature ovaries compared with non-migratory take-offs. These findings advance our understanding of the take-off behavior of S. furcifera and thus provide a basis for the accurate prediction and management of the migratory dynamics of this pest.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1908-1917"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into paternity skew in a polyandrous social wasp. 多雄性社会黄蜂亲子关系偏斜的新见解。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-28 DOI: 10.1111/1744-7917.13343

Sarah E Orr, Nicole A Hedrick, Kayla A Murray, Abhinav K Pasupuleti, Michael A D Goodisman

{"title":"Novel insights into paternity skew in a polyandrous social wasp.","authors":"Sarah E Orr, Nicole A Hedrick, Kayla A Murray, Abhinav K Pasupuleti, Michael A D Goodisman","doi":"10.1111/1744-7917.13343","DOIUrl":"10.1111/1744-7917.13343","url":null,"abstract":"Females of many species are polyandrous. However, polyandry can give rise to conflict among individuals within families. We examined the level of polyandry and paternity skew in the common eastern yellowjacket wasp, Vespula maculifrons, in order to gain a greater understanding of conflict in social insects. We collected 10 colonies of V. maculifrons and genotyped workers and prereproductive queens at highly variable microsatellite markers to assign each to a patriline. Genotypic data revealed evidence of significant paternity skew among patrilines. In addition, we found that patrilines contributed differentially to caste production (worker vs. queen), suggesting an important role for reproductive conflict not previously discovered. We also investigated if patterns of paternity skew and mate number varied over time. However, we found no evidence of changes in levels of polyandry when compared to historical data dating back almost 40 years. Finally, we measured a suite of morphological traits in individuals from the most common and least common patrilines in each colony to test if males that showed highly skewed reproductive success also produced offspring that differed in phenotype. Our data revealed weak correlation between paternity skew and morphological phenotype of offspring sired by different males, suggesting no evidence of evolutionary tradeoffs at the level investigated. Overall, this study is the first to report significant paternity and caste-associated skew in V. maculifrons, and to investigate the phenotypic consequences of skew in a social wasp. Our results suggest that polyandry can have important consequences on the genetic and social structure of insect societies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1876-1888"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation in the triosephosphate isomerase gene of the fall armyworm and its distribution across China. 秋梢虫三糖磷酸异构酶基因的遗传变异及其在中国的分布。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-27 DOI: 10.1111/1744-7917.13348

Xin-Yue Liang, Lei Zhang, Hong-Ran Li, Xiao-Ping Niu, Yu-Tao Xiao

{"title":"Genetic variation in the triosephosphate isomerase gene of the fall armyworm and its distribution across China.","authors":"Xin-Yue Liang, Lei Zhang, Hong-Ran Li, Xiao-Ping Niu, Yu-Tao Xiao","doi":"10.1111/1744-7917.13348","DOIUrl":"10.1111/1744-7917.13348","url":null,"abstract":"The fall armyworm (FAW), Spodoptera frugiperda, has colonized and caused consistent damage in the Eastern hemisphere. The identification of various FAW strains is essential for developing precise prevention and control measures. The triosephosphate isomerase (Tpi) gene is recognized as an effective marker closely linked to FAW subpopulations. However, most current studies primarily focus on the comparison of variations in specific gene sites of this gene. In this study, we conducted full-length sequencing of the Tpi genes from 5 representative FAW groups. Our findings revealed that the Tpi genes varied in length from 1220 to 1420 bp, with the primary variation occurring within 4 introns. Notably, the exon lengths remained consistent, at 747 bp, with 37 observed base variations; however, no amino acid variations were detected. Through sequence alignment, we identified 8 stable variation sites that can be used to distinguish FAW strains in the Eastern hemisphere. Additionally, we performed strain identification on 1569 FAW samples collected from 19 provinces in China between 2020 and 2021. The extensive analysis indicated the absence of the rice strain in the samples. Instead, we only detected the presence of the corn strain and the Zambia strain, with the Zambia strain being distributed in a very low proportion (3.44%). Furthermore, the corn strain could be further categorized into 2 subgroups. This comprehensive study provides a valuable reference for enhancing our understanding of FAW population differentiation and for improving monitoring and early warning efforts.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1984-1997"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae). 群居繁殖的甲虫 Necrodes littoralis（Staphylinidae: Silphinae）在腐肉上形成的幼虫群中的竞争、合作和亲代效应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-13 DOI: 10.1111/1744-7917.13353

Natalia Lis, Anna Mądra-Bielewicz, Jędrzej Wydra, Szymon Matuszewski

{"title":"Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae).","authors":"Natalia Lis, Anna Mądra-Bielewicz, Jędrzej Wydra, Szymon Matuszewski","doi":"10.1111/1744-7917.13353","DOIUrl":"10.1111/1744-7917.13353","url":null,"abstract":"Aggregations of juveniles are dominant forms of social life in some insect groups. Larval societies are shaped by competitive and cooperative interactions of the larvae, in parallel with parental effects. Colonies of necrophagous larvae are excellent systems to study these relationships. Necrodes littoralis (Staphylinidae: Silphinae), a carrion beetle that colonizes cadavers of large vertebrates, forms massive juvenile aggregations. By spreading over carrion anal and oral exudates, the beetles form the feeding matrix, in which the heat is produced and by which adults presumably affect the fitness of the larvae. We predict that exploitative competition shapes the behavior of N. littoralis larvae in their aggregations. However, cooperative interactions may also operate in these systems due mainly to the benefits of collective exodigestion. Moreover, indirect parental effects (i.e., formation of the feeding matrix) probably modulate larval interactions within the aggregations. By manipulating parental effects (present/absent) and larval density (0.02-1.9 larvae/g of meat), we found a strong negative group-size effect on fitness components of N. littoralis, in colonies with parental effects over almost the entire density range, and in colonies without parental effects for densities larger than 0.5 larva/g. This was accompanied by positive group-size effects in terms of development time (it shortened with larval density) and thermogenesis (it increased with larval density). A pronounced positive group-size effect on juvenile fitness was found only in colonies without parental effects and only in the low-density range. These results support the hypothesis that larval societies of N. littoralis are shaped by exploitation competition.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1918-1929"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive toxicity assessment of nanodiamond on Blaps polychresta: implications and novel findings. 纳米金刚石对 Blaps polychresta 的综合毒性评估：影响和新发现。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-26 DOI: 10.1111/1744-7917.13357

Marwa Saad, Nabila Selim, Lamia M El-Samad

{"title":"Comprehensive toxicity assessment of nanodiamond on Blaps polychresta: implications and novel findings.","authors":"Marwa Saad, Nabila Selim, Lamia M El-Samad","doi":"10.1111/1744-7917.13357","DOIUrl":"10.1111/1744-7917.13357","url":null,"abstract":"With the increasing development of nanomaterials, the use of nanodiamonds (NDs) has been broadly manifested in many applications. However, their high penetration into the ecosystem indubitably poses remarkable toxicological risks. This paper investigates the toxic effects of NDs on the darkling beetle, Blaps polychresta Forskal, 1775 (Coleoptera: Tenebrionidae). Survival analysis was carried out by monitoring the beetles for 30 d after the injection of four different doses of NDs. A dose of 10.0 mg NDs/g body weight, causing less than 50% mortality effect, was assigned in the analysis of the different organs of studied beetles, including testis, ovary, and midgut. Structural and ultrastructural analyses were followed using light, TEM, and SEM microscopes. In addition, a variety of stress markers and enzyme activities were assessed using spectrophotometric methods. Furthermore, cell viability and DNA damage were evaluated using cytometry and comet assay, respectively. Compared to the control group, the NDs-treated group was exposed to various abnormalities within all the studied organs as follows. Significant disturbances in enzyme activities were accompanied by an apparent dysregulation in the antioxidant system. The flow cytometry results indicated a substantial decrease of viable cells along with a rise of apoptotic and necrotic cells. The comet assay demonstrated a highly increased level of DNA damage. Likewise, histological analyses accentuated the same findings showing remarkable deformities in the studied organs. Prominently, the research findings substantially contribute for the first time to evaluating the critical effects of NDs on B. polychresta, adopted as the bioindicator in this paper.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1838-1863"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fasting as a precursor to high-fat diet enhances mitochondrial resilience in Drosophila melanogaster. 禁食是高脂饮食的前奏，可增强黑腹果蝇线粒体的恢复能力。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-21 DOI: 10.1111/1744-7917.13355

Florence Hunter-Manseau, Simon B Cormier, Rebekah Strang, Nicolas Pichaud

{"title":"Fasting as a precursor to high-fat diet enhances mitochondrial resilience in Drosophila melanogaster.","authors":"Florence Hunter-Manseau, Simon B Cormier, Rebekah Strang, Nicolas Pichaud","doi":"10.1111/1744-7917.13355","DOIUrl":"10.1111/1744-7917.13355","url":null,"abstract":"Changes in diet type and nutrient availability can impose significant environmental stress on organisms, potentially compromising physiological functions and reproductive success. In nature, dramatic fluctuations in dietary resources are often observed and adjustments to restore cellular homeostasis are crucial to survive this type of stress. In this study, we exposed male Drosophila melanogaster to two modulated dietary treatments: one without a fasting period before exposure to a high-fat diet and the other with a 24-h fasting period. We then investigated mitochondrial metabolism and molecular responses to these treatments. Exposure to a high-fat diet without a preceding fasting period resulted in disrupted mitochondrial respiration, notably at the level of complex I. On the other hand, a short fasting period before the high-fat diet maintained mitochondrial respiration. Generally, transcript abundance of genes associated with mitophagy, heat-shock proteins, mitochondrial biogenesis, and nutrient sensing pathways increased either slightly or significantly following a fasting period and remained stable when flies were subsequently put on a high-fat diet, whereas a drastic decrease of almost all transcript abundances was observed for all these pathways when flies were exposed directly to a high-fat diet. Moreover, mitochondrial enzymatic activities showed less variation after the fasting period than the treatment without a fasting period. Overall, our study sheds light on the mechanistic protective effects of fasting prior to a high-fat diet and highlights the metabolic flexibility of Drosophila mitochondria in response to abrupt dietary changes and have implication for adaptation of species to their changing environment.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1770-1788"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0