Insect Science最新文献_第6页

AMPK regulates HIF-1α to induce pupal diapause in the cotton bollworm, Helicoverpa armigera. AMPK调控HIF-1α诱导棉铃虫蛹滞育。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2025-05-06 DOI: 10.1111/1744-7917.70068

Zhiren Su, Songshan Jiang, Wei-Hua Xu

{"title":"AMPK regulates HIF-1α to induce pupal diapause in the cotton bollworm, Helicoverpa armigera.","authors":"Zhiren Su, Songshan Jiang, Wei-Hua Xu","doi":"10.1111/1744-7917.70068","DOIUrl":"10.1111/1744-7917.70068","url":null,"abstract":"Diapause is an adaptive strategy employed by insects to endure adverse environmental conditions and is characterized by reduced metabolic activity, primarily due to a decreased respiratory rate. AMP-activated protein kinase (AMPK) serves as an intracellular energy regulator, modulating energy metabolism in response to metabolic fluctuations. However, its role in pupal diapause of the cotton bollworm, Helicoverpa armigera, remains unclear. In this study, we found that AMPK and its active form, P-AMPK, are highly expressed in diapause-destined pupae. Furthermore, activation of AMPK delayed the development of nondiapause-destined pupae, suggesting a critical role for AMPK in the regulation of pupal diapause in H. armigera. Manipulating AMPK activity in H. armigera epidermal (HaEpi) cells and pupae significantly influenced the expression of hypoxia-inducible factor-1α (HIF-1α), which our laboratory previously reported as a key inducer of pupal diapause through the reduction of mitochondrial activity in H. armigera. Histone deacetylase 4 (HDAC4), a shuttle protein phosphorylated by AMPK which translocates between the cytoplasm and the nucleus, was found to exhibit significantly higher expression in diapause-destined pupal brains compared to their nondiapause counterparts. AMPK in both HaEpi cells and pupae positively regulated the protein levels of P-HDAC4 by binding to the HDAC4 promoter. Additionally, HDAC4 was shown to enhance HIF-1α expression in diapause-destined individuals. HDAC4 binds to and deacetylates heat shock protein 70 (HSP70), and reduced acetylation of HSP70 was found to significantly elevate HIF-1α protein levels. The AMPK-HIF-1α signaling pathway appears to play a pivotal role in reducing mitochondrial activity and facilitating diapause induction in H. armigera pupae.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1283-1297"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foraging by predatory ants: A review. 食肉蚂蚁的觅食：综述。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-21 DOI: 10.1111/1744-7917.13461

Alain Dejean, Jérôme Orivel, Xim Cerdá, Frédéric Azémar, Bruno Corbara, Axel Touchard

{"title":"Foraging by predatory ants: A review.","authors":"Alain Dejean, Jérôme Orivel, Xim Cerdá, Frédéric Azémar, Bruno Corbara, Axel Touchard","doi":"10.1111/1744-7917.13461","DOIUrl":"10.1111/1744-7917.13461","url":null,"abstract":"In this review, we show that predatory ants have a wide range of foraging behavior, something expected given their phylogenetic distance and the great variation in their colony size, life histories, and nesting habitats as well as prey diversity. Most ants are central-place foragers that detect prey using vision and olfaction. Ground-dwelling species can forage solitarily, the ancestral form, but generally recruit nestmates to retrieve large prey or a group of prey. Typically, ants are omnivorous, but some species are strict predators preying on detritivorous invertebrates or arthropod eggs, while those specialized on termites or other ants often have scouts that localize their target and then trigger a raid. They can use compounds that ease this task, including chemical insignificance, mimicry, and venoms triggering submissive behavior. Army ants include 8 Dorylinae and some species from other subfamilies, all having wingless queens and forming raids. Dorylinae from the Old World migrate irregularly to new nesting sites. The foraging of most New World species that prey on the brood of other ants is regulated by their biological cycle that alternates between a \"nomadic phase\" when the colony relocates between different places and a \"stationary phase\" when the colony stays in a bivouac constituting a central place. Among arboreal ants, dominant species forage in groups, detecting prey visually, but can use vibrations, particularly when associated with myrmecophytes. Some species of the genera Allomerus and Azteca use fungi to build a gallery-shaped trap with small holes under which they hide to ambush prey.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1096-1118"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient CRISPR-mediated genome editing can be initiated by embryonic injection but not by ovarian delivery in the beetle Tribolium castaneum. 在甲虫Tribolium castaneum中，胚胎注射可以启动高效的CRISPR介导的基因组编辑，而卵巢注射则不行。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-20 DOI: 10.1111/1744-7917.13447

Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Xing-Ce Wang, Tong-Xian Liu, Hong-Gang Tian

{"title":"Efficient CRISPR-mediated genome editing can be initiated by embryonic injection but not by ovarian delivery in the beetle Tribolium castaneum.","authors":"Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Xing-Ce Wang, Tong-Xian Liu, Hong-Gang Tian","doi":"10.1111/1744-7917.13447","DOIUrl":"10.1111/1744-7917.13447","url":null,"abstract":"The clustered regularly interspaced small palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9)-mediated gene editing technology has revolutionized the study of fundamental biological questions in various insects. Diverse approaches have been developed to deliver the single-guide RNA (sgRNA) and Cas9 to the nucleus of insect embryos or oocytes to achieve gene editing, including the predominant embryonic injection methods and alternative protocols through parental ovary delivery. However, a systematic comparative study of these approaches is limited, especially within a given insect. Here, we focused on revealing the detailed differences in CRISPR/Cas9-mediated gene editing between the embryo and ovary delivery methods in the beetle Tribolium castaneum, using the cardinal and tyrosine hydroxylase (TH) as reporter genes. We demonstrated that both genes could be efficiently edited by delivering Cas9/sgRNA ribonucleoproteins to the embryos by microinjection, leading to the mutant phenotypes and indels in the target gene sites. Next, the Cas9/sgRNA complex, coupled with a nanocarrier called Branched Amphiphilic Peptide Capsules (BAPC), were delivered to the ovaries of parental females to examine the efficacy of BAPC-mediated gene editing. Although we observed that a small number of beetles' progeny targeting the cardinal exhibited the expected white-eye phenotype, unexpectedly, no target DNA indels were found following subsequent sequencing analysis. In addition, we adopted a novel approach termed \"direct parental\" CRISPR (DIPA-CRISPR). However, we still failed to find gene-editing events in the cardinal or TH gene-targeted insects. Our results indicate that the conventional embryonic injection of CRISPR is an effective method to initiate genome editing in T. castaneum. However, it is inefficient by the parental ovary delivery approach.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1185-1200"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and functional characterization of circulating hemocytes in Tribolium castaneum larvae. 蓖麻幼虫循环血细胞的形态和功能特征。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-03 DOI: 10.1111/1744-7917.13455

Suisui Wang, Shiyuan Miao, Yusi Li, Jianhui Wang, Chengjun Li, Yujie Lu, Bin Li

{"title":"Morphological and functional characterization of circulating hemocytes in Tribolium castaneum larvae.","authors":"Suisui Wang, Shiyuan Miao, Yusi Li, Jianhui Wang, Chengjun Li, Yujie Lu, Bin Li","doi":"10.1111/1744-7917.13455","DOIUrl":"10.1111/1744-7917.13455","url":null,"abstract":"Hemocytes are pivotal in the immune response of insects against invasive pathogens. However, our knowledge of hemocyte types and their specific function in Tribolium castaneum, an increasingly important Coleoptera model insect in various research fields, remains limited. Presently, a combination of morphological criteria and dye-staining properties were used to characterize hemocyte types from T. castaneum larvae, and 4 distinct types were identified: granulocytes, oenocytoids, plasmatocytes and prohemocytes. Following different immune challenges, the total hemocyte counts declined rapidly in the initial phase (at 2 h), then increased over time (at 4 and 6 h) and eventually returned to the naive state by 24 h post-injection. Notably, the morphology of granulocytes underwent dramatic changes, characterized by an expansion of the surface area and an increased production of pseudopods, and with the number of granulocytes rising significantly through mitotic division. Granulocytes and plasmatocytes, the main hemocyte types in T. castaneum larvae, can phagocytose bacteria or latex beads injected into the larval hemolymph in vivo. Furthermore, these hemocytes participate in the encapsulation and melanization processes in vitro, forming capsules to encapsulate and melanize nickel-nitrilotriacetic acid (Ni-NTA) beads. This study provides the first comprehensive characterization of circulating hemocytes in T. castaneum larvae, offering valuable insights into cell-mediated immunity in response to bacterial infection and the injection of latex beads. These results deepen our understanding of the cellular response mechanisms in T. castaneum larvae and lay a solid foundation for subsequent investigations of the involvement of T. castaneum hemocytes in combating pathogens.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1331-1350"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved fungal effector NLS1 suppresses Lepidoptera insect immunity by targeting the host defense protein Hdd11. 保守的真菌效应因子 NLS1 通过靶向宿主防御蛋白 Hdd11 抑制鳞翅目昆虫的免疫力。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-09 DOI: 10.1111/1744-7917.13454

Yulong Wang, Ying Dong, Kexin Liu, Gen Li, Jing Cheng, Yin Cao, Yang Yang, Li Qin, Bo Huang

{"title":"Conserved fungal effector NLS1 suppresses Lepidoptera insect immunity by targeting the host defense protein Hdd11.","authors":"Yulong Wang, Ying Dong, Kexin Liu, Gen Li, Jing Cheng, Yin Cao, Yang Yang, Li Qin, Bo Huang","doi":"10.1111/1744-7917.13454","DOIUrl":"10.1111/1744-7917.13454","url":null,"abstract":"Entomopathogenic fungi have been widely used as the main mycoinsecticide for controlling agricultural and forest pests. The effector molecules of these mycopathogens have evolved to adapt to their hosts. The role of fungal effectors in evading the host immune system in insects remains mainly unclear. We characterized the widely distributed fungal effector necrosis-inducing-like secreted protein 1 (NLS1) in the entomopathogenic fungus Metarhizium robertsii. Our findings revealed the presence of M. robertsii NLS1 (MrNLS1) in host hemocytes during the early stage of hemocoel infection. MrNLS1 knock down (ΔMrNLS1) reduced fungal pathogenicity during infection and altered the expression of host immune genes. The molecular docking results and the yeast 2-hybrid assay confirmed that MrNLS1 interacts with the host defense protein Hdd11. The phylogenetic analysis indicated that Hdd11 is conserved across a broad range of Lepidoptera species. Knock down of hdd11 in Helicoverpa armigera, Bombyx mori, and Galleria mellonella markedly suppressed their immune responses against M. robertsii. However, no significant difference was observed in the mean lethal time between hdd11-knockdown Lepidoptera species infected with ΔMrNLS1 and those infected with wild-type M. robertsii. Therefore, in Lepidoptera insects, Hdd11 is essential for fungal defense. In conclusion, M. robertsii infects Lepidoptera insects by targeting host Hdd11 through its protein MrNLS1, thereby suppressing the host immune response. Our findings clarify the molecular mechanisms underlying fungal infection pathogenesis.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1298-1310"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphis craccivora (Hemiptera: Aphididae) synthesizes juvenile hormone III via a pathway involving epoxidation followed by esterification, potentially providing an epoxidation active site for the synthesis of juvenile hormone SB3. Aphis craccivora（半翅目：蚜科）通过环氧化然后酯化的途径合成幼虫激素 III，这可能为幼虫激素 SB3 的合成提供了一个环氧化活性位点。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-04 DOI: 10.1111/1744-7917.13450

Haolin Li, Xue Kong, Yan Fang, Jiangan Hou, Wenjie Zhang, Yongheng Zhang, Jiguang Wei, Xuesheng Li

{"title":"Aphis craccivora (Hemiptera: Aphididae) synthesizes juvenile hormone III via a pathway involving epoxidation followed by esterification, potentially providing an epoxidation active site for the synthesis of juvenile hormone SB3.","authors":"Haolin Li, Xue Kong, Yan Fang, Jiangan Hou, Wenjie Zhang, Yongheng Zhang, Jiguang Wei, Xuesheng Li","doi":"10.1111/1744-7917.13450","DOIUrl":"10.1111/1744-7917.13450","url":null,"abstract":"Juvenile hormones (JHs) play a crucial role in regulating development and reproduction in insects. Most insects predominantly synthesize JH III, which typically involves esterification followed by epoxidation, lepidopteran insects use a pathway of epoxidation followed by esterification. Although hemipteran insects have JH III and JH skipped bisepoxide III (JH SB3), the synthesis pathway and key epoxidases remain unclear. This study was conducted on Aphis craccivora, and demonstrated that corpora allata, microsomes, Ac-CYP15C1, and Ac-JHAMT catalyze JH III production in vitro, establishing the pathway of epoxidation followed by esterification. These findings were further confirmed through RNA interference and molecular docking. The presence of JH III and JH SB3 in A. craccivora was identified, and their synthesis pathway was elucidated as follows: Ac-CYP15C1 oxidizes farnesic acid to JH A, followed by methylation to JH III by Ac-JHAMT, possibly providing an epoxidation site on the second carbon for JH SB3. This alteration may significantly contribute to the differentiation and functional diversification of JH types in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1311-1330"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrative strategy used by the aphid Uroleucon formosanum to counter host sesquiterpene lactone defense: Insights from combined genomic and transcriptomic analysis. 蚜虫 Uroleucon formosanum 对抗寄主倍半萜内酯防御的综合策略：基因组和转录组联合分析的启示。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-30 DOI: 10.1111/1744-7917.13452

Jing-Bo Li, Li-Yun Jiang, Ge-Xia Qiao, Jing Chen

{"title":"An integrative strategy used by the aphid Uroleucon formosanum to counter host sesquiterpene lactone defense: Insights from combined genomic and transcriptomic analysis.","authors":"Jing-Bo Li, Li-Yun Jiang, Ge-Xia Qiao, Jing Chen","doi":"10.1111/1744-7917.13452","DOIUrl":"10.1111/1744-7917.13452","url":null,"abstract":"Insect herbivores adapt and develop strategies to counteract plant chemical defenses. The aphid Uroleucon formosanum is a serious sap-sucking pest that infests lettuces containing toxic sesquiterpene lactones (STLs). Herein, we employed a combination of genome sequencing and RNA-seq transcriptome profiling to understand the mechanisms underlying phytotoxin tolerance in U. formosanum. We generated the first chromosome-level genome assembly for U. formosanum, with a total size of 453.26 Mb and a scaffold N50 of 33.22 Mb. Comparative genomic analyses revealed an enrichment of signals for positive selection and gene family expansion in immune-related pathways. Specifically, the expanded set of heat shock protein 70 (HSP70) genes showed upregulation after treatment with lactucin, suggesting that they may play a role in the immune response against STLs. The expression of takeout-like genes and cuticle-associated genes was also significantly increased in the lactucin-treated samples. Additionally, 53 cytochrome P450 monooxygenase, 30 carboxylesterase, 19 glutathione S-transferase, 32 uridine diphosphate glycosyltransferase and 63 ATP-binding cassette (ABC) transporter genes were identified in the U. formosanum genome. CYP4C1, CYP6A13 and 7 ABC genes were strongly upregulated in response to lactucin treatment, indicating the involvement of detoxifying enzymes in the tolerance of U. formosanum to STLs. Our findings suggest that the cuticle barrier, immune response and enzyme-mediated metabolic detoxification jointly enhance the tolerance of U. formosanum to phytotoxins and promote its adaptation to host plants. This study presents a valuable genomic resource and provides insights into insect adaptation to plant chemical challenges and future technological developments for pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1155-1173"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in within-plant oviposition preferences and immature survival between Orius predators and the importance of spatial availability of prey. Orius捕食者在植物内的产卵偏好和幼虫存活率的差异以及猎物空间可用性的重要性。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-31 DOI: 10.1111/1744-7917.13465

Angelos Mouratidis, Christiaan Bootsma, Marcel Dicke, Gerben J Messelink

{"title":"Differences in within-plant oviposition preferences and immature survival between Orius predators and the importance of spatial availability of prey.","authors":"Angelos Mouratidis, Christiaan Bootsma, Marcel Dicke, Gerben J Messelink","doi":"10.1111/1744-7917.13465","DOIUrl":"10.1111/1744-7917.13465","url":null,"abstract":"Oviposition preferences of plant-feeding predators remain a complex topic, as such omnivores choose oviposition sites by assessing both plant characteristics and the quality and quantity of nearby animal food sources. Orius predators are omnivores that oviposit endophytically, thus plant characteristics play an important role in their oviposition choices. In this study, we assessed the oviposition and foraging preferences of O. laevigatus and O. majusculus on vegetative and flowering chrysanthemum plants, and assessed the survival of their offspring on differently aged tissues. Our results show a preference of O. laevigatus for young and tender chrysanthemum tissues, where the survival of the nymphs was longer on a plant diet. In contrast, O. majusculus selected older plant parts when laying its eggs, and nymphs did not survive long on any of the plant tissues offered. The foraging activity of Orius females for animal prey (Ephestia kuehniella eggs) did not reveal any specific pattern for either of the two predators. Furthermore, we tested the plasticity of the within-plant oviposition preferences of O. laevigatus, by offering sentinel prey (E. kuehniella eggs) on distinct plant parts. We found that more eggs were laid in older plant tissue when animal prey was offered lower on the plant. Overall, our findings show that oviposition choices of Orius predators are based on a dynamic interplay between plant characteristics, presence of animal and/or floral food sources among other factors, and that differences may well occur between closely related species based on the importance of plant resources in their diet.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1415-1426"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NompC regulates locomotion and touch sensation in Bactrocera dorsalis. NompC 调节背脊乳杆菌的运动和触觉。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-11-03 DOI: 10.1111/1744-7917.13459

Hong-Ai Su, Miao-Miao Zhang, Hui Wei, Hai-Kuo Yu, Yong-Yue Lu, Yi-Xiang Qi

{"title":"NompC regulates locomotion and touch sensation in Bactrocera dorsalis.","authors":"Hong-Ai Su, Miao-Miao Zhang, Hui Wei, Hai-Kuo Yu, Yong-Yue Lu, Yi-Xiang Qi","doi":"10.1111/1744-7917.13459","DOIUrl":"10.1111/1744-7917.13459","url":null,"abstract":"No mechanoreceptor potential C (NompC) is a major mechanotransduction channel with an important role in sensing of external mechanical stimuli by insects, which help these organisms to avoid injury and adapt to environmental changes. To explore the biological functions of NompC in Bactrocera dorsalis, a notorious agricultural pest, we successfully generated NompC knockout strains using clustered regularly interspaced small palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9) technology. BdorNompC knockout led to an adult lethal phenotype, with approximately 100% mortality at 3 d after eclosion. Morphological observation revealed that the legs and wings of BdorNompC knockout insects were deformed, while behavioral assays showed that the locomotion was impaired in both adults and larvae, relative to that of the wild-type strain. Moreover, BdorNompC knockout reduced gentle-touch response in larvae. These results suggest that BdorNompC is critical for B. dorsalis survival, and that this mechanosensation channel represents a potential new target for pest control agents. Our findings also represent novel evidence indicating that insect NompC is involved in modulating adult wing and leg morphology.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1201-1212"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A newly identified Y chromosome gene obp-Y is required for sperm storage in female Zeugodacus tau (Diptera: Tephritidae). 新发现的 Y 染色体基因 obp-Y 是雌性 Zeugodacus tau（双翅目：Tephritidae）精子储存所必需的。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-30 DOI: 10.1111/1744-7917.13453

Peipei Liu, Ziniu Li, Imran Afzal, Qiuyuan Zhang, Jiao Qiao, Dong Wu, Hongyu Zhang

{"title":"A newly identified Y chromosome gene obp-Y is required for sperm storage in female Zeugodacus tau (Diptera: Tephritidae).","authors":"Peipei Liu, Ziniu Li, Imran Afzal, Qiuyuan Zhang, Jiao Qiao, Dong Wu, Hongyu Zhang","doi":"10.1111/1744-7917.13453","DOIUrl":"10.1111/1744-7917.13453","url":null,"abstract":"In the organisms with XX/XY sex chromosomes, Y chromosome is unique to males and plays an important role in male reproductive development. The study of Y chromosome genes will contribute to the development of pest genetic prevention and control technology. In this study, we identified 9 Y chromosome genes in Zeugodacus tau (Diptera: Tephritidae), including gene 16222. Protein structure analysis showed that 16222 was highly similar to odorant binding protein, and thus gene 16222 was named obp-Y. Obp-Y knockout (KO) significantly reduced hatching rate of offspring. Sperm detection results showed that obp-Y KO did not affect sperm number in the testes or sperm transfer during mating. We further examined the storage of sperms in females, and found that sperms in females mating with wild-type males began to transfer from spermathecal ducts to the spermathecae at hour 0 after the end of mating (AEM), and at 0-24 h AEM, the sperm count in the spermathecae gradually increased. However, no sperms were observed in spermathecae of females mating with mutant males at hours 0, 4, 8, 24 and 48 AEM. In summary, this study revealed that Y chromosome gene obp-Y was necessary for the storage of sperms in females. Our findings not only provide theoretical basis for elucidating the function of the Y chromosome, but also offer a molecular target for the genetic control over Z. tau.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1213-1226"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0