Insect Science最新文献_第6页

Let-7 microRNA targets BmCentrin to modulate the development and functionality of the middle silk gland in the silkworm, Bombyx mori. Let-7 microRNA 以 BmCentrin 为靶标，调节家蚕中丝腺的发育和功能。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-29 DOI: 10.1111/1744-7917.13380

Yankun Xu, Na Du, Lili Xu, Lu Zhao, Ting Fan, Tianqi Wei, Qian Pu, Shiping Liu

{"title":"Let-7 microRNA targets BmCentrin to modulate the development and functionality of the middle silk gland in the silkworm, Bombyx mori.","authors":"Yankun Xu, Na Du, Lili Xu, Lu Zhao, Ting Fan, Tianqi Wei, Qian Pu, Shiping Liu","doi":"10.1111/1744-7917.13380","DOIUrl":"10.1111/1744-7917.13380","url":null,"abstract":"The silk gland of the silkworm Bombyx mori serves as a valuable model for investigating the morphological structure and physiological functions of organs. Previous studies have demonstrated the notable regulatory role of let-7 microRNA in the silk gland, but its specific molecular mechanism remains to be elucidated across different segments of this organ. In this study, we further investigated the functional mechanism of let-7 in the middle silk gland (MSG). The MSG of a let-7 knockout strain was analyzed using a combined proteomic and metabolomic technique, revealing the enrichment of differential proteins and metabolites in the DNA synthesis and energy metabolism pathways. BmCentrin was identified as a novel target gene of let-7 in the MSG, and its downregulation inhibited the proliferation of BmN4-SID1 cells, which is exactly opposite to the role of let-7 in these cells. CRISPR/Cas9 genome editing and transgenic technologies were employed to manipulate BmCentrin in the MSG. Knockout of BmCentrin led to severe MSG atrophy, whereas the overexpression of BmCentrin resulted in beaded MSG. Further measurements of these knockout or overexpression strains revealed significant changes in the expression levels of sericin protein genes, the weight of the cocoon and the mechanical properties of the silk. Investigating the biological role of BmCentrin in the silk gland offers valuable insights for elucidating the molecular mechanisms by which let-7 controls silk gland development and silk protein synthesis in the silkworm.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"95-114"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer, Ostrinia furnacalis. 一种具有双重碳水化合物识别结构域的 C 型凝集素在亚洲玉米螟的先天免疫反应中发挥作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-21 DOI: 10.1111/1744-7917.13382

Er-Tao Li, Jia-Yue Ji, Wei-Jie Kong, Dong-Xu Shen, Cai Li, Chun-Ju An

{"title":"A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer, Ostrinia furnacalis.","authors":"Er-Tao Li, Jia-Yue Ji, Wei-Jie Kong, Dong-Xu Shen, Cai Li, Chun-Ju An","doi":"10.1111/1744-7917.13382","DOIUrl":"10.1111/1744-7917.13382","url":null,"abstract":"C-type lectins (CTLs) act as pattern recognition receptors (PRRs) to initiate the innate immune response in insects. A CTL with dual carbohydrate recognition domains (CRDs) (named immulectin-4 [IML-4]) was selected from the Ostrinia furnacalis transcriptome dataset for functional studies. We cloned the full-length complementary DNA of O. furnacalis IML-4 (OfIML-4). It encodes a 328-residue protein with a Glu-Pro-Asn (EPN) and Gln-Pro-Asp (QPD) motifs in 2 CRDs, respectively. OfIML-4 messenger RNA levels increased significantly upon the bacterial and fungal infection. Recombinant OfIML-4 (rIML-4) and its individual CRDs (rCRD1 and rCRD2) exhibited the binding ability to various microorganisms including Escherichia coli, Micrococcus luteus, Pichia pastoris, and Beauveria bassiana, and the cell wall components including lipopolysaccharide from E. coli, peptidoglycan from M. luteus or Bacillus subtilis, and curdlan from Alcaligenes faecalis. The binding further induced the agglutination of E. coli, M. luteus, and B. bassiana in the presence of calcium, the phagocytosis of Staphylococcus aureus by the hemocytes, in vitro encapsulation and melanization of nickel-nitrilotriacetic acid beads, and a significant increase in phenoloxidase activity of plasma. In addition, rIML-4 significantly enhanced the phagocytosis, nodulation, and resistance of O. furnacalis to B. bassiana. Taken together, our results suggest that OfIML-4 potentially works as a PRR to recognize the invading microorganisms, and functions in the innate immune response in O. furnacalis.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"172-192"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escort: a system for rapid and long-term tracking of multiple insect objects. 护航：快速、长期跟踪多个昆虫目标的系统。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-06-17 DOI: 10.1111/1744-7917.13407

Chengshi Wu, Jin Ge, Bin Han, Hengjing Lan, Xian Zhou, Zhuxi Ge, Weichan Cui, Xiaofeng Liu, Xianhui Wang

引用次数: 0

miR-2940-1 is involved in the circadian regulation of oviposition in Aedes albopictus. miR-2940-1 参与了白纹伊蚊排卵的昼夜节律调节。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-03-31 DOI: 10.1111/1744-7917.13362

Xiaolin Xiao, Ling Kong, Zhensheng Xie, Hongkai Liu, Lijun Cai, Siyu Zhao, Jiayong Zhou, Shuang Liu, Jing Wu, Yiming Wu, Peilin Wu, Anthony A James, Xiao-Guang Chen

{"title":"miR-2940-1 is involved in the circadian regulation of oviposition in Aedes albopictus.","authors":"Xiaolin Xiao, Ling Kong, Zhensheng Xie, Hongkai Liu, Lijun Cai, Siyu Zhao, Jiayong Zhou, Shuang Liu, Jing Wu, Yiming Wu, Peilin Wu, Anthony A James, Xiao-Guang Chen","doi":"10.1111/1744-7917.13362","DOIUrl":"10.1111/1744-7917.13362","url":null,"abstract":"The vast majority of all global species have circadian rhythm cycles that allow them to adapt to natural environments. These regular rhythms are regulated by core clock genes and recent studies have also implicated roles for microRNAs in this regulation. Oviposition is an important circadian behavior in the reproductive cycle of insect vectors of diseases, and little is known about the rhythm or its regulation in mosquitoes. Aedes albopictus is a diurnal mosquito that transmits arboviruses and is the major cause of outbreaks of dengue fever in China. We analyzed the oviposition rhythm patterns of A. albopictus under different light/dark conditions and show that the mosquitoes have an oviposition peak between zeitgeber time 9 (ZT 9) and ZT 12. Furthermore, the antagomir-mediated knockdown of expression of the microRNA miR-2940-1 affected the oviposition rhythm of A. albopictus. These data support the conclusion that miR-2940-1 is involved in the regulation of oviposition rhythm in A. albopictus and provide a foundation for using oviposition rhythms as a new target for vector mosquito control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"69-79"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological immunology: do sexual attraction and immunity trade-off through a desaturase? 生态免疫学：性吸引力和免疫力是否通过去饱和酶进行权衡？

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-20 DOI: 10.1111/1744-7917.13379

Ke Gao, Wout van der Heide, Daphne Muijderman, Sarah Nichols, Carmen Karwal, Peter Kuperus, Astrid T Groot

{"title":"Ecological immunology: do sexual attraction and immunity trade-off through a desaturase?","authors":"Ke Gao, Wout van der Heide, Daphne Muijderman, Sarah Nichols, Carmen Karwal, Peter Kuperus, Astrid T Groot","doi":"10.1111/1744-7917.13379","DOIUrl":"10.1111/1744-7917.13379","url":null,"abstract":"Given the limited availability of resources in nature, sexual attractiveness may trade off with immunocompetence, as the immunocompetence handicap hypothesis (ICHH) posits. In invertebrates, a direct link between trade-offs through hormonal/molecular effectors in sexual signals and immunity has not been found so far. Here, we assessed how variation in sexual signals affected parasite infection in two sex pheromone selected lines of the moth Chloridea virescens: an attractive line with a low ratio of 16:Ald/Z11-16:Ald and an unattractive line with a high ratio. When infecting these lines with an apicomplexan parasite, we found that the attractive Low line was significantly more susceptible to the parasite infection than the unattractive High line. Since the ratio difference between these two lines is determined by a delta-11-desturase, we hypothesized that this desaturase may have a dual role, i.e., in the quality of the sexual signal as well as an involvement in immune response, comparable to testosterone in vertebrates. However, when we used CRISPR/cas9 to knockout delta-11-desturase in the attractive Low line, we found that the pheromonal phenotype did change to that of the High line, but the infection susceptibility did not. Notably, when checking the genomic location of delta-11-desaturase in the C. virescens, we found that mucin is adjacent to delta-11-desaturase. When comparing the mucin sequences in both lines, we found four nonsynonymous SNPs in the coding sequence, as well as intronic variation between the two lines. These differences suggest that genetic hitchhiking may explain the variation in susceptibility to parasitic infection.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"290-300"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P450 gene CYP321A8 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda. P450 基因 CYP6a13 是田间蛙类对杀虫剂产生交叉抗药性的原因。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-21 DOI: 10.1111/1744-7917.13376

Yumei Chen, Yongjie Cen, Yu Liu, Yanan Peng, Yiguang Lin, Qili Feng, Yong Xiao, Sichun Zheng

{"title":"P450 gene CYP321A8 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda.","authors":"Yumei Chen, Yongjie Cen, Yu Liu, Yanan Peng, Yiguang Lin, Qili Feng, Yong Xiao, Sichun Zheng","doi":"10.1111/1744-7917.13376","DOIUrl":"10.1111/1744-7917.13376","url":null,"abstract":"Continuous and long-term use of traditional and new pesticides can result in cross-resistance among pest populations in different fields. Study on the mechanism of cross-resistance and related genes will help resistance management and field pest control. In this study, the pesticide-resistance mechanism in Spodoptera frugiperda (FAW) was studied with field populations in 3 locations of South China. Field FAW populations were highly resistant to traditional insecticides, chlorpyrifos (organophosphate) and deltamethrin (pyrethroid), and had higher levels of cytochrome P450 activity than a non-resistant laboratory strain. Inhibition of P450 activity by piperonyl butoxide significantly increased the sensitivity of resistant FAW in 3 locations to chlorpyrifos, deltamethrin and chlorantraniliprole (amide), a new type of insecticide, suggesting that P450 detoxification is a critical factor for insecticide resistance in field FAW populations. Transcriptomic analysis indicated that 18 P450 genes were upregulated in the field FAW populations collected in 3 regions and in 2 consecutive years, with CYP321A8, the most significantly upregulated one. Knockdown of CYP321A8 messenger RNA by RNA interference resulted in an increased sensitivity to the 3 tested insecticides in the field FAW. Enzyme activity and molecular docking analyses indicated that CYP321A8 enzyme was able to metabolize the 3 tested insecticides and interact with 8 other types of insecticides, confirming that CYP321A8 is a key cross-resistance gene with a wide range of substrates in the field FAW populations across the different regions and can be used as a biomarker and target for management of FAW insecticide resistance in fields.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"227-242"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnaporthe oryzae infection triggers rice resistance to brown planthopper through the influence of jasmonic acid on the flavonoid biosynthesis pathway. 通过茉莉酸对类黄酮生物合成途径的影响，Magnaporthe oryzae感染引发了水稻对褐飞虱的抗性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-15 DOI: 10.1111/1744-7917.13378

Su Chen, Zhihuan Tao, Yanjie Shen, Rui Yang, Siyuan Yan, Zixu Chen, Bo Sun, Xiaofang Yang

{"title":"Magnaporthe oryzae infection triggers rice resistance to brown planthopper through the influence of jasmonic acid on the flavonoid biosynthesis pathway.","authors":"Su Chen, Zhihuan Tao, Yanjie Shen, Rui Yang, Siyuan Yan, Zixu Chen, Bo Sun, Xiaofang Yang","doi":"10.1111/1744-7917.13378","DOIUrl":"10.1111/1744-7917.13378","url":null,"abstract":"In agroecosystems, plants are constantly exposed to attack from diverse herbivorous insects and microbes, and infestation with one species may change the plant defense response to other species. In our investigation of the relationships among rice plants, the brown planthopper Nilaparvata lugens (Stål) and the rice blast fungus Magnaporthe oryzae, we observed a significant increase in the resistance of rice treated with rice blast to N. lugens, as evidenced by improved plant survival rates in a small population resistance study. Subsequent transcriptome data analysis revealed that the rice blast fungus can induce the expression of genes in the jasmonic acid (JA) and flavonoid pathways. Similar to the flavonoid pathway, the JA pathway also contains 2 types of genes that exhibit similar and opposite trends in response to N. lugens and rice blast. Among these genes, the osjaz1 mutant and the osmyc2 mutant were phenotypically confirmed to positively and negatively regulate rice resistance to N. lugens and rice blast, respectively. Subsequent mass spectrometry and quantification experiments showed that the exogenous application of methyl jasmonate (MeJA) can induce the accumulation of eriodictyol, naringenin and quercetin, as well as the expression of OsF3H, Os4CL5 and OsCHI in the flavonoid pathway. This suggests a close connection between the JA pathway and the flavonoid pathway. However, OsF3'H, which negatively regulates rice resistance to N. lugens and rice blast, did not show increased expression. Phenotypic and molecular experiments confirmed that OsMYC2 can bind to and inhibit the expression of OsF3'H, thus revealing the mechanism of rice resistance to N. lugens after treatment with rice blast. These findings will deepen our understanding of the interactions among rice, N. lugens and rice blast.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"243-259"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bimodal alarm signals modulate responses to monomodal alarm signals in Camponotus modoc carpenter ants. 双模报警信号会调节 Camponotus modoc 木匠蚁对单模报警信号的反应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-15 DOI: 10.1111/1744-7917.13375

Asim Renyard, Gerhard Gries

{"title":"Bimodal alarm signals modulate responses to monomodal alarm signals in Camponotus modoc carpenter ants.","authors":"Asim Renyard, Gerhard Gries","doi":"10.1111/1744-7917.13375","DOIUrl":"10.1111/1744-7917.13375","url":null,"abstract":"Distressed western carpenter ants, Camponotus modoc, produce alarm pheromone and substrate-borne vibrations. The alarm pheromone attracts nestmates but the effects of vibratory signals, or of bimodal pheromonal and vibratory signals, are not known. Worker ants of two Camponotus congeners reportedly stand still (\"freeze\") or run fast in response to engineered drumming vibrations inputted on plastic, but many responses to ant-produced vibratory signals on wood have not yet been investigated. Generally, orientating toward signalers under vertebrate predator attack seems maladaptive and not beneficial to ant colonies. We tested the hypotheses (1) that vibratory alarm signals cause freezing, rapid running but not attraction of nestmates, and (2) that bimodal alarm signals modulate responses to monomodal alarm signals, thereby possibly reducing predation risk. Laser Doppler vibrometry recordings revealed that the ants' vibratory signals readily propagate through ant nest lamellae, and thus quickly inform nest mates of perceived threats. With a speaker modified to record and deliver vibratory signals, we obtained drumming signals of distressed ants on a Douglas fir veneer, and bioassayed signal effects on ants in an arena with a suspended veneer floor. In response playback of vibratory signals, ants ran rapidly, or froze, but did not approach the vibratory signals. Exposed to alarm pheromone, ants frequently visited the pheromone source. However, concurrently exposed to both alarm pheromone and vibratory signals, ants visited the pheromone source less often but spent more time \"frozen.\" The ants' modulated responses to bimodal signals seem adaptive but the reproductive fitness benefits are still to be quantified.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"343-355"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long non-coding RNAs as promising targets for controlling disease vector mosquitoes. 长非编码核糖核酸是控制病媒蚊子的有效目标。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-23 DOI: 10.1111/1744-7917.13383

Yujiao Han, Qian Pu, Ting Fan, Tianqi Wei, Yankun Xu, Lu Zhao, Shiping Liu

{"title":"Long non-coding RNAs as promising targets for controlling disease vector mosquitoes.","authors":"Yujiao Han, Qian Pu, Ting Fan, Tianqi Wei, Yankun Xu, Lu Zhao, Shiping Liu","doi":"10.1111/1744-7917.13383","DOIUrl":"10.1111/1744-7917.13383","url":null,"abstract":"Hematophagous female mosquitoes are important vectors of numerous devastating human diseases, posing a major public health threat. Effective prevention and control of mosquito-borne diseases rely considerably on progress in understanding the molecular mechanisms of various life activities, and accordingly, the molecules that regulate the various life activities of mosquitoes are potential targets for implementing future vector control strategies. Many long non-coding RNAs (lncRNAs) have been identified in mosquitoes and significant progress has been made in determining their functions. Here, we present a comprehensive overview of the research advances on mosquito lncRNAs, including their molecular identification, function, and interaction with other non-coding RNAs, as well as their synergistic regulatory roles in mosquito life activities. We also highlight the potential roles of competitive endogenous RNAs in mosquito growth and development, as well as in insecticide resistance and virus-host interactions. Insights into the biological functions and mechanisms of lncRNAs in mosquito life activities, viral replication, pathogenesis, and transmission will contribute to the development of novel drugs and safe vaccines.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"24-41"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-role regulator of a novel miR-3040 in photoperiod-mediated wing dimorphism and wing development in green peach aphid. 新型 miR-3040 在光周期介导的绿桃蚜翅二态性和翅发育中的双重作用调节因子

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-10 DOI: 10.1111/1744-7917.13377

Yun-Hui Zhang, Xin Qian, Xin Zong, Shi-Heng An, Shuo Yan, Jie Shen

{"title":"Dual-role regulator of a novel miR-3040 in photoperiod-mediated wing dimorphism and wing development in green peach aphid.","authors":"Yun-Hui Zhang, Xin Qian, Xin Zong, Shi-Heng An, Shuo Yan, Jie Shen","doi":"10.1111/1744-7917.13377","DOIUrl":"10.1111/1744-7917.13377","url":null,"abstract":"Wing dimorphism is regarded as an important phenotypic plasticity involved in the migration and reproduction of aphids. However, the signal transduction and regulatory mechanism of wing dimorphism in aphids are still unclear. Herein, the optimal environmental conditions were first explored for inducing winged offspring of green peach aphid, and the short photoperiod was the most important environmental cue to regulate wing dimorphism. Compared to 16 L:8 D photoperiod, the proportion of winged offspring increased to 90% under 8 L:16 D photoperiod. Subsequently, 5 differentially expressed microRNAs (miRNAs) in aphids treated with long and short photoperiods were identified using small RNA sequencing, and a novel miR-3040 was identified as a vital miRNA involved in photoperiod-mediated wing dimorphism. More specifically, the inhibition of miR-3040 expression could reduce the proportion of winged offspring induced by short photoperiod, whereas its activation increased the proportion of winged offspring under long photoperiod. Meanwhile, the expression level of miR-3040 in winged aphids was about 2.5 times that of wingless aphids, and the activation or inhibition of miR-3040 expression could cause wing deformity, revealing the dual-role regulator of miR-3040 in wing dimorphism and wing development. In summary, the current study identified the key environmental cue for wing dimorphism in green peach aphid, and the first to demonstrate the dual-role regulator of miR-3040 in photoperiod-mediated wing dimorphism and wing development.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"80-94"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140904129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0