Insect Science最新文献_第7页

Larval diet impacts black soldier fly (Diptera: Stratiomyidae) thermal tolerance and preference. 幼虫饮食对黑兵蝇（双翅目：Stratiomyidae）热耐受性和喜好性的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-05 DOI: 10.1111/1744-7917.13429

Chujun Li, Travis W Rusch, Amy J Dickerson, Aaron M Tarone, Jeffery K Tomberlin

{"title":"Larval diet impacts black soldier fly (Diptera: Stratiomyidae) thermal tolerance and preference.","authors":"Chujun Li, Travis W Rusch, Amy J Dickerson, Aaron M Tarone, Jeffery K Tomberlin","doi":"10.1111/1744-7917.13429","DOIUrl":"https://doi.org/10.1111/1744-7917.13429","url":null,"abstract":"Thermal tolerance and preference are key parameters impacting agricultural production systems. In this study, the impact of larval diet on black soldier fly thermal tolerance and preference across life-stages and sexes was examined. Larvae were fed either a low-protein high-carbohydrate synthetic diet (i.e., P7C35), a high-protein low-carbohydrate synthetic diet (i.e., P35C7), or the Gainesville diet (i.e., C) as a control and reference. Our results demonstrate that the impacts of larval diet on black soldier fly thermal tolerance and preference could be stage and sex specific. The mean heat knockdown temperatures (HKT) ranged between 46.6 and 47.9 °C. Synthetic diets resulted in greater HKT and the difference decreased form larvae (e.g., ∼1 °C) to adults (e.g., ∼0.2 °C). The mean chill-coma recovery time (CCRT) ranged between 8.3 and 21.6 min. Not much differences were detected between diets, but CCRT became longer from larvae to adults. The mean thermal preference ranged between 13.6 and 29.5 °C. Larvae fed synthetic diets preferred much lower temperatures than the control diet. A bimodal distribution was observed for adults regardless of sex. Differences on body mass, lipid, and protein contents were detected among diets; however, more research should be done before any conclusions can be linked to their thermal traits. These findings highlight the importance of considering the ingredients and nutritional makeup of larval diets when optimizing temperature management protocols for mass production of black soldier flies. Conversely, specific diets can be developed to promote survival under extreme rearing temperatures.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific and interspecific resource partitioning between bumblebee workers and males related to nectar quantity and quality. 熊蜂工蜂和雄蜂之间的种内和种间资源分配与花蜜的数量和质量有关。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-05 DOI: 10.1111/1744-7917.13425

Xiao-Fang Jin, Zhong-Ming Ye, Yong-Deng He, Chun-Feng Yang, Michael Orr, Arong Luo, Paul Williams, Chao-Dong Zhu

{"title":"Intraspecific and interspecific resource partitioning between bumblebee workers and males related to nectar quantity and quality.","authors":"Xiao-Fang Jin, Zhong-Ming Ye, Yong-Deng He, Chun-Feng Yang, Michael Orr, Arong Luo, Paul Williams, Chao-Dong Zhu","doi":"10.1111/1744-7917.13425","DOIUrl":"https://doi.org/10.1111/1744-7917.13425","url":null,"abstract":"Bumblebees are important pollinators for many natural and agricultural systems in temperate regions. Interspecific and intraspecific variation in floral resource preferences have been proposed to influence bumblebee community structure. In particular, sexual dimorphism is a major source of intraspecific niche variation. Although interspecific resource partitioning is well studied, few studies have explored the intraspecific dynamics between workers and males. Here, we report a study on a total of 11 528 workers and 2220 males of 14 bumblebee species recorded over 5 years in the Hengduan Mountains of Southwest China. We first compared the potential for interspecific and intraspecific competition between workers and males using visitation records and resource partitioning indices (overlap index). We then evaluated the influence of nectar traits on flower preference, including nectar volume and the levels of hexose, sucrose and 10 essential amino acids (EAAs). We found that the niche overlap between intraspecific workers and males was higher than that between different species, and temporal overlap alone did not strongly determine diet overlap. Males of most species preferred flowers with high levels of EAAs and hexose, whereas workers of some species preferred flowers with high nectar volume and sucrose levels. This study suggests that there is floral resource partitioning among bumblebee species, and between workers and males, which may play a key role in alleviating interspecific and intraspecific competition. These findings also provide a useful guide for which kinds of plants might be most valuable for bumblebees, especially the understudied males, in this biodiversity hotspot.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feeding behavior and hormoligosis associated with imidacloprid resistance in Asian citrus psyllid, Diaphorina citri. 柑桔木虱对吡虫啉抗性的摄食行为和茧虫病。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-15 DOI: 10.1111/1744-7917.13293

Xue Dong Chen, Justin George, Lauren M Diepenbrock, Hunter Gossett, Guoping Liu, Jawwad A Qureshi, Lukasz L Stelinski

{"title":"Feeding behavior and hormoligosis associated with imidacloprid resistance in Asian citrus psyllid, Diaphorina citri.","authors":"Xue Dong Chen, Justin George, Lauren M Diepenbrock, Hunter Gossett, Guoping Liu, Jawwad A Qureshi, Lukasz L Stelinski","doi":"10.1111/1744-7917.13293","DOIUrl":"10.1111/1744-7917.13293","url":null,"abstract":"Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid, Diaphorina citri Kuwayama, which serves as vector of phytopathogens causing citrus greening. However, development of resistance to neonicotinoids among populations of D. citri has coincided with occasional control failures in the field. The objectives of this research were to (1) survey current levels of imidacloprid resistance in Florida citrus; (2) compare feeding behavior between imidacloprid-resistant and susceptible D. citri using electrical penetration graph recordings, and (3) investigate the possible amplification of insecticide hormoligosis associated with resistance. Field surveys confirmed that the susceptibility of D. citri populations to imidacloprid has decreased in commercial Florida citrus groves compared with a laboratory-susceptible population. Following 12 generations of selection, resistance to imidacloprid increased by 438 fold compared with the susceptible strain. Imidacloprid-susceptible D. citri feeding on citrus exhibited significantly more bouts associated with intercellular pathway (C), phloem penetration (D), phloem salivation (E1), and nonprobing (Np) activities than imidacloprid-resistant counterparts. However, there were no differences observed in the frequency or duration of phloem ingestion or xylem feeding between susceptible and resistant D. citri. There was no statistical difference in fecundity between resistant and susceptible strains. However, the fecundity of imidacloprid-susceptible female D. citri treated with a sublethal concentration of imidacloprid (LC25) increased significantly compared with controls, while such hormoligosis was less pronounced among imidacloprid-resistant psyllids. Our results suggest that imidacloprid-resistant psyllids may cease feeding sooner than susceptible counterparts following sublethal exposure to this insecticide, indicative of a behavioral resistance mechanism.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Object use in insects. 昆虫中的物体用途。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-13 DOI: 10.1111/1744-7917.13275

Chao Wen, Cai Wang, Xiaoli Guo, Hongyu Li, Haijun Xiao, Junbao Wen, Shikui Dong

引用次数: 0

Spirotetramat reduces fitness of the spotted-wing Drosophila, Drosophila suzukii. Spirotetramat降低斑点翼果蝇的适应性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-18 DOI: 10.1111/1744-7917.13283

Jing Yang, Justin Flaven-Pouchon, Yiwen Wang, Bernard Moussian

{"title":"Spirotetramat reduces fitness of the spotted-wing Drosophila, Drosophila suzukii.","authors":"Jing Yang, Justin Flaven-Pouchon, Yiwen Wang, Bernard Moussian","doi":"10.1111/1744-7917.13283","DOIUrl":"10.1111/1744-7917.13283","url":null,"abstract":"With climate change, the spotted-wing Drosophila (SWD, Drosophila suzukii) invades a great number of fruit production regions worldwide. A plethora of insecticides are being applied for management of this pest. As expected, SWD develops resistance against some potent insecticides or is rather insensitive to some others. Therefore, there is an urgent need to identify and characterize alternative insecticides to control SWD populations. Here, we have studied the effects of the orally applied inhibitor of the fatty acid synthesis pathway spirotetramat on SWD fitness with respect to fecundity and surface barrier function at different stages. In our experiments, we applied spirotetramat mixed with baker's yeast and showed that females and males were not repelled by effective concentrations of this insecticide. We found that spirotetramat, by trend, lowers egg numbers laid by fed females. These eggs rapidly desiccate, and only a few larvae hatch. Spirotetramat is lethal to larvae and reduces survival of adult flies under low-humidity conditions. Taken together, based on our data, we propose to use yeast supplemented with spirotetramat and additional SWD-specific attractants in traps in non-crop areas in order to eradicate SWD populations before they infest crop production sites.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excitation of the abdominal ganglion affects the electrophysiological activity of indirect flight muscles of the honeybee Apis mellifera. 腹神经节的兴奋影响蜜蜂间接飞行肌的电生理活动。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-31 DOI: 10.1111/1744-7917.13290

Haojia Ding, Shaoze Yan

{"title":"Excitation of the abdominal ganglion affects the electrophysiological activity of indirect flight muscles of the honeybee Apis mellifera.","authors":"Haojia Ding, Shaoze Yan","doi":"10.1111/1744-7917.13290","DOIUrl":"10.1111/1744-7917.13290","url":null,"abstract":"Our understanding of the nervous tissues that affect the wing flapping of insects mainly focuses on the brain, but wing flapping is a rhythmic movement related to the central pattern generator in the ventral nerve cord. To verify whether the neural activity of the abdominal ganglion of the honeybee (Apis mellifera) affects the flapping-wing flight, we profiled the response characteristics of indirect flight muscles to abdominal ganglion excitation. Strikingly, a change in the neural activity of ganglion 3 or ganglion 4 has a stronger effect on the electrophysiological activity of indirect flight muscles than that of ganglion 5. The electrophysiological activity of vertical indirect flight muscles is affected more by the change in neural activity of the abdominal ganglion than that of lateral indirect flight muscles. Moreover, the change in neural activity of the abdominal ganglion mainly causes the change in the muscular activity of indirect wing muscles, but the activity patterns change relatively little and there is little change in the complicated details. This work improves our understanding of the neuroregulatory mechanisms associated with the flapping-wing flight of honeybees.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71423411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egg-associated secretions from the brown planthopper (Nilaparvata lugens) activate rice immune responses. 褐飞虱(Nilaparvata lugens)的卵相关分泌物激活水稻免疫反应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-27 DOI: 10.1111/1744-7917.13303

Jing Li, Shuai Li, Jing Li, Xinyang Tan, Zhichang Zhao, Lei Jiang, Ary A Hoffmann, Jichao Fang, Rui Ji

{"title":"Egg-associated secretions from the brown planthopper (Nilaparvata lugens) activate rice immune responses.","authors":"Jing Li, Shuai Li, Jing Li, Xinyang Tan, Zhichang Zhao, Lei Jiang, Ary A Hoffmann, Jichao Fang, Rui Ji","doi":"10.1111/1744-7917.13303","DOIUrl":"10.1111/1744-7917.13303","url":null,"abstract":"The brown planthopper (BPH, Nilaparvata lugens) is a notorious sap-sucking insect pest that damages rice (Oryza sativa) plants throughout Asia. During BPH feeding, saliva enters rice plant tissues, whereas during oviposition egg-associated secretions (EAS) are deposited in damaged plant tissue. Dynamic changes in rice to planthopper salivary effectors have been widely reported. However, the effects of EAS from planthopper on rice immunity remains largely unexplored. In this study, we found that both infestation of rice by gravid BPH female adults and treatment with the EAS elicited a strong and rapid accumulation of jasmonic acid (JA), JA-isoleucine, and hydrogen peroxide in rice. EAS enhanced plant defenses not only in rice but also in tobacco, and these impaired the performance of BPH on rice, as well as the performance of aphids and whiteflies on tobacco. High-throughput proteome sequencing of EAS led to 110 proteins being identified and 53 proteins with 2 or more unique peptides being detected. Some proteins from BPH EAS were also found in the salivary proteome from herbivores, suggesting potential evolutionary conservation of effector functions across feeding and oviposition; however, others were only identified in EAS, and these are likely specifically related to oviposition. These findings point to novel proteins affecting interactions between planthoppers and rice during oviposition, providing an additional source of information for effector studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138444565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA let-7 targets BmCDK1 to regulate cell proliferation and endomitosis of silk gland in the silkworm, Bombyx mori. MicroRNA let-7 以 BmCDK1 为靶标，调控家蚕丝腺的细胞增殖和内吸。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-12-05 DOI: 10.1111/1744-7917.13302

Wei Wang, Linshengzhe Ji, Xinyuan Jing, Ping Zhao, Qingyou Xia

{"title":"MicroRNA let-7 targets BmCDK1 to regulate cell proliferation and endomitosis of silk gland in the silkworm, Bombyx mori.","authors":"Wei Wang, Linshengzhe Ji, Xinyuan Jing, Ping Zhao, Qingyou Xia","doi":"10.1111/1744-7917.13302","DOIUrl":"10.1111/1744-7917.13302","url":null,"abstract":"MicroRNAs play critical roles in multiple developmental processes in insects. Our previous study showed that CRISPR/Cas9-mediated knock down of the microRNA let-7 in silkworms increased the size of larvae and silk glands, thereby improving the silk production capacity. In this study, we elucidate the molecular mechanism underlying of let-7 regulates growth. Identification of differentially expressed genes in response to let-7 knock down revealed enrichment of pathways associated with cell proliferation and DNA replication. let-7 dysregulation affected the cell cycle and proliferation of the Bombyx mori cell line BmN. Dual-luciferase and target site mutation assays showed that BmCDK1 is a direct target gene of let-7, with only 1 binding site on its 3'-untranslated region. RNA interference of BmCDK1 inhibited cell proliferation, but this effect was counteracted by co-transfection with let-7 antagomir. Moreover, let-7 knock down induced BmCDK1 expression and promoted cell proliferation in multiple tissues, and further induced endomitosis in the silk gland in vivo. Knock down of BmCDK1 resulted in abnormal formation of a new epidermis, and larval development was arrested at the 2nd or 3rd molt stage. Taken together, our results demonstrated that BmCDK1 is a novel target of let-7 in cell fate determination, possessing potential for improving silk yield in silkworm.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel diagnostic gene region for distinguishing between two pest fruit flies: Bactrocera tryoni (Froggatt) and Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae). 一种区分两种害虫果蝇的新诊断基因区域:特氏小实蝇(Froggatt)和新肱骨小实蝇(Hardy)(双翅目:蝗科)。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-22 DOI: 10.1111/1744-7917.13299

Melissa L Starkie, Elizabeth V Fowler, Alexander M Piper, Xiaocheng Zhu, Pauline Wyatt, David Gopurenko, Matt N Krosch, Francesca Strutt, Karen F Armstrong, Hamish Patrick, Mark K Schutze, Mark J Blacket

{"title":"A novel diagnostic gene region for distinguishing between two pest fruit flies: Bactrocera tryoni (Froggatt) and Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae).","authors":"Melissa L Starkie, Elizabeth V Fowler, Alexander M Piper, Xiaocheng Zhu, Pauline Wyatt, David Gopurenko, Matt N Krosch, Francesca Strutt, Karen F Armstrong, Hamish Patrick, Mark K Schutze, Mark J Blacket","doi":"10.1111/1744-7917.13299","DOIUrl":"10.1111/1744-7917.13299","url":null,"abstract":"Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes, geographic distributions, and host ranges. The need to differentiate between the two species is critical for accurate pest status assessment, management, biosecurity, and maintenance of reference colonies. While morphologically similar, adults may be separated based on subtle characters; however, some characters exhibit intraspecific variability, creating overlap between the two species. Additionally, there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between B. neohumeralis and B. tryoni; therefore, ambiguous samples remain undiagnosed. Here we report the first molecular marker that can consistently distinguish between B. tryoni and B. neohumeralis. Our diagnostic region consists of two adjacent single nucleotide polymorphisms (SNPs) within the pangolin (pan) gene region. We confirmed the genotypes of each species are consistent across their distributional range, then developed a tetra-primer amplification refractory mutation system (ARMS) PCR assay for rapid diagnosis of the species. The assay utilizes four primers in multiplex, with two outer universal primers, and two internal primers: one designed to target two adjacent SNPs (AA) present in B. tryoni and the other targeting adjacent SNPs present in B. neohumeralis (GG). The assay accurately discriminates between the two species, but their SNP genotypes are shared with other nontarget tephritid fruit fly species. Therefore, this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches; maintaining pure colony lines; and in Sterile Insect Technique management responses.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138290871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The neglect of nonnative orthopterans as potential invaders: A call for awareness. 忽视非本地直翅目昆虫作为潜在入侵者：呼吁提高认识。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-12 DOI: 10.1111/1744-7917.13277

Anna K Kulessa, Paride Balzani, Ismael Soto, Antonín Kouba, David Renault, Ali Serhan Tarkan, Phillip J Haubrock

引用次数: 0