Insect Science最新文献_第7页

A CRISPR/Cas9 gene-editing platform for the rice leaffolder, Cnaphalocrocis medinalis. 稻卷叶稻的CRISPR/Cas9基因编辑平台

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-10 DOI: 10.1111/1744-7917.70360

Juncheng Yan, Bingpeng Liu, Hui Lu, Shuai Zhan, Dongbin Chen, Jun Xu

引用次数: 0

Functional differentiation of ground-dwelling beetle communities as an adaptation to fragile alluvial fan habitats in arid regions. 干旱区地表甲虫群落的功能分化及其对脆弱冲积扇生境的适应

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-07 DOI: 10.1111/1744-7917.70353

Wenbin Yang, Shuyu Zhang, Min Zhao, Qiaoling Ma, Yuan Wang, Xue Ma, Guijun Yang

{"title":"Functional differentiation of ground-dwelling beetle communities as an adaptation to fragile alluvial fan habitats in arid regions.","authors":"Wenbin Yang, Shuyu Zhang, Min Zhao, Qiaoling Ma, Yuan Wang, Xue Ma, Guijun Yang","doi":"10.1111/1744-7917.70353","DOIUrl":"https://doi.org/10.1111/1744-7917.70353","url":null,"abstract":"Alluvial fans are typically fragile and unstable landforms in arid regions, and their bioenvironmental coupling mechanisms remain unclear. The aims of this study were to test the hypothesis that elevation gradients drive beetle functional group differentiation through environmental filtering, reveal the core strategies of functional group adaptation to fragile habitats, and provide a theoretical basis for arid ecological restoration. Five transects were established along the alluvial fan elevation gradient, and ground-dwelling beetles were collected using pitfall trapping. Species and functional diversity analyses were conducted, and functional groups were classified using hierarchical clustering. The associations between functional traits and environmental factors were analyzed using RLQ and fourth-corner analysis. A total of 8251 ground-dwelling beetles were collected, and community diversity varied significantly with elevation. The first two axes of the RLQ explained 91.28% of total co-inertia. Feeding habits, activity rhythms, hind legs, antennae, and head width were significantly correlated with environmental variables. The five functional groups showed a gradient distribution from the fan apex to the fan margin: \"broad-headed nocturnal saprophagous group → nocturnal carnivorous group → nocturnal phytophagous group → diurnal phytophagous group → long-legged and long-antennae diurnal phytophagous group.\" Combined with partial least squares structural equation modeling (PLS-SEM), this study confirmed the rule that alluvial fan instability regulates habitat heterogeneity through elevation gradients, which in turn drives beetle functional group differentiation. Functional groups can serve as indicators of habitat stability. This study deepens understanding of insect community assembly mechanisms in fragile landforms and provides scientific support for arid ecosystem protection and restoration.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148906058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphid and endosymbiont sensitivity to heatwaves is misaligned across life stages. 蚜虫和内共生体对热浪的敏感性在生命的各个阶段是不一致的。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-02 DOI: 10.1111/1744-7917.70349

Evatt Chirgwin, Qiong Yang, Paul A Umina, Xuan Cheng, Xinyue Gu, Alex Gill, Ary A Hoffmann

{"title":"Aphid and endosymbiont sensitivity to heatwaves is misaligned across life stages.","authors":"Evatt Chirgwin, Qiong Yang, Paul A Umina, Xuan Cheng, Xinyue Gu, Alex Gill, Ary A Hoffmann","doi":"10.1111/1744-7917.70349","DOIUrl":"https://doi.org/10.1111/1744-7917.70349","url":null,"abstract":"Heritable endosymbionts will shape how many insect pests respond to climate warming. Aphids are globally important pests that almost universally depend on the obligate symbiont Buchnera aphidicola, which can constrain host thermal tolerance, while additional secondary symbionts may further modify heat responses. Importantly, aphid-symbiont interactions are developmentally dynamic, with physiological functions and phenotypic effects changing as aphids develop. However, it remains unclear whether aphids and their endosymbionts are most vulnerable to heat stress at the same developmental stages, or whether mismatches in thermal sensitivity shape fitness outcomes. We addressed this gap by testing how heatwaves typical of southern Australia affect the invasive cereal pest, the Russian wheat aphid (Diuraphis noxia), and two endosymbionts (Buchnera aphidicola and Rickettsiella viridis) across developmental stages. Using a factorial design (endosymbiont status × life stage × temperature), we exposed early nymphs or adults to either moderate or extreme heatwave conditions-representative of their invaded range-and quantified survival, post-exposure longevity, fecundity, and symbiont density in treated individuals and their offspring. Heatwaves had a disproportionately strong effect on the nymph stage compared with adults, primarily through reduced fecundity. In contrast, endosymbiont responses were strongest following adult exposure, including Buchnera aphidicola suppression, in adults and/or their offspring. These results reveal a life-stage mismatch in thermal vulnerability between hosts and symbionts, with implications for physiological responses to climate extremes.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Honeybees achieve precise bilateral balance in pollen loads. 蜜蜂在花粉负荷上达到精确的双边平衡。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-09-01 DOI: 10.1111/1744-7917.70348

Shuang-Qin Hu, Fang-Yun Liao, Yu-Xiang Wang, Chen Yang, Xing-Chao Yan, Xian-Bing Xie, Xu-Jiang He

引用次数: 0

Sensory-evoked locomotion selectively enhances transient and sustained food odor representations in a mushroom body dopaminergic circuit in Drosophila. 感觉诱发运动选择性地增强了蘑菇体多巴胺能回路中短暂和持续的食物气味表征。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-28 DOI: 10.1111/1744-7917.70351

Qiantao Lv, Yunming Gao, Yaokai Yang, Jing Ning, Yi Sun

{"title":"Sensory-evoked locomotion selectively enhances transient and sustained food odor representations in a mushroom body dopaminergic circuit in Drosophila.","authors":"Qiantao Lv, Yunming Gao, Yaokai Yang, Jing Ning, Yi Sun","doi":"10.1111/1744-7917.70351","DOIUrl":"https://doi.org/10.1111/1744-7917.70351","url":null,"abstract":"Animals must continuously adapt their behavior to dynamic sensory environments and internal motor states. In Drosophila and other invertebrates, the mushroom body is key to this behavioral flexibility, with dopaminergic neurons (DANs) and mushroom body output neurons (MBONs) forming a core circuit for context-dependent action selection. Yet, how olfactory cues and locomotor states are jointly integrated and transformed across the DAN-MBON circuit is unclear. Using single- and dual-color two-photon calcium and dopamine imaging, we found distinct representations in the γ1 compartment. In response to prolonged food odor delivery, γ1 DANs exhibit transient responses tightly locked to odor onset and offset, whereas MBONs maintain a sustained response throughout odor delivery. Moreover, while spontaneous locomotion elicits faithful DAN calcium dynamics and dopamine release without clearly affecting MBONs, odor-evoked locomotion selectively amplifies the transient and sustained olfactory responses in DANs and MBONs, respectively, facilitating context-dependent olfactory-motor integration. Linear encoding models reveal that DANs broadly integrate diverse olfactory and locomotor variables, and exhibit higher variability than MBONs. We propose a model where dopaminergic transients serve as context-switch signals, integrating environmental and locomotor contexts to flexibly modulate sustained MBON output and guide behavior.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of specialized metabolites in plant‒insect interactions: A review. 特化代谢物在植物-昆虫相互作用中的作用：综述。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-28 DOI: 10.1111/1744-7917.70346

Xiaoli Liu, Jiancai Li

{"title":"The role of specialized metabolites in plant‒insect interactions: A review.","authors":"Xiaoli Liu, Jiancai Li","doi":"10.1111/1744-7917.70346","DOIUrl":"https://doi.org/10.1111/1744-7917.70346","url":null,"abstract":"Plants have evolved sophisticated defense mechanisms against phytophagous insects through long-term coevolution, while insects have developed diverse adaptive strategies to overcome plant defenses. Plant specialized metabolites (SMs), also known as secondary metabolites, comprise a diverse group of chemical compounds, many of which function as chemical defenses against insect herbivores. A better understanding of the roles of plant SMs in plant-insect interactions should facilitate development of novel biopesticides, the exploitation of pest-resistance traits for crops, and the development of sustainable strategies of insecticide resistance managements. In this review, we summarize the regulation mechanisms underlying the biosynthesis and accumulation of plant SMs in response to insect herbivory, discuss the multiple effects of plant SMs on insect behavior, physiology, and development, and describe the adaptive mechanisms that enable insects to cope with plant chemical defenses. Finally, we highlight future research directions and discuss the potential applications of plant SMs in sustainable pest management. This review provides a comprehensive overview of plant‒insect interactions mediated by plant SMs and offers perspectives for developing environmentally friendly pest management strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148839473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A horizontally acquired pantothenate gene drives vitellogenin's benefit to whitefly symbiosis persistence. 水平获得的泛酸基因驱动卵黄蛋白原对白蝇共生持久性的益处。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-20 DOI: 10.1111/1744-7917.70345

Xiang Sun, Bing-Qi Liu, Zhan-Bo Chen, Chu-Qiao Li, Xing-Ye Li, Jun-Bo Luan

{"title":"A horizontally acquired pantothenate gene drives vitellogenin's benefit to whitefly symbiosis persistence.","authors":"Xiang Sun, Bing-Qi Liu, Zhan-Bo Chen, Chu-Qiao Li, Xing-Ye Li, Jun-Bo Luan","doi":"10.1111/1744-7917.70345","DOIUrl":"10.1111/1744-7917.70345","url":null,"abstract":"Insects can survive in nutrient-poor environments owing to nutritional symbionts that produce vitamins and essential amino acids (EAAs). Nonetheless, how symbionts actively benefit from this nutritional symbiosis remains incompletely understood. Horizontally transferred genes (HTGs) expressed in bacteriocytes can function autonomously or cooperatively with symbionts to biosynthesize EAAs or B vitamins. We previously demonstrated that the horizontally transferred panBC and the symbiont Portiera cooperatively synthesize vitamin B5 (pantothenate), thereby enhancing whitefly fecundity. Beyond supporting host reproduction, this nutritional symbiosis also confers a fitness advantage on the symbionts. We further showed that induction of autophagy reduces symbiont abundance in bacteriocytes, whereas inhibition of autophagy increases it. Here, we found that silencing panBC reduced Portiera titer, impaired whitefly oogenesis, and disrupted vitellogenin (Vg) localization in ovarioles and bacteriocytes by modulating juvenile hormone biosynthesis. Pantothenate supplementation restored Vg localization in panBC RNAi whiteflies. Furthermore, silencing either Vg or panBC induced autophagy in bacteriocytes, and rapamycin-induced autophagy decreased symbiont titers. Taken together, our findings demonstrate that pantothenate, synthesized cooperatively by the horizontally transferred panBC and Portiera, regulates Vg localization in whiteflies and that Vg, in turn, protects symbionts from autophagic degradation. Our study suggests that horizontally transferred genes and symbionts jointly contribute to an important role of Vg in maintaining and shaping the evolution of insect-symbiont interactions. This study advances our understanding of how HTGs contribute to the persistence of nutritional symbiosis.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural TMEM145 GPCR inhibitors enhancing clothianidin susceptibility in Nilaparvata lugens. 天然TMEM145 GPCR抑制剂增强褐飞虱对噻虫胺的敏感性。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-20 DOI: 10.1111/1744-7917.70347

Guijian Zhang, Xiangqian Chang, Shun He, Liang Lv, Hu Wan

{"title":"Natural TMEM145 GPCR inhibitors enhancing clothianidin susceptibility in Nilaparvata lugens.","authors":"Guijian Zhang, Xiangqian Chang, Shun He, Liang Lv, Hu Wan","doi":"10.1111/1744-7917.70347","DOIUrl":"https://doi.org/10.1111/1744-7917.70347","url":null,"abstract":"While G protein-coupled receptors (GPCRs) are recognized as key upstream regulators of insecticide resistance in various agricultural pests, effective strategies to exploit these receptors for practical pest control remain largely undeveloped. In this study, we screened and identified TMEM145 as a novel GPCR significantly upregulated in clothianidin resistant Nilaparvata lugens. RNAi-mediated knockdown of TMEM145 suppressed the expression of the detoxification gene CYP6ER1 and the transcription factor AP-1, successfully restoring clothianidin susceptibility with a lethal dose ratio (LCR50) of 1.78. Through structure-based virtual screening of 4000 bioactive molecules targeting TMEM145, we mined pectolinarin as a potential inhibitor exhibiting a high binding affinity of -10.945 kcal/mol. Its structural scaffold, apigenin, was identified as an alternative with a binding affinity of -8.223 kcal/mol. Experimental validation confirmed that chemical inhibition by these flavonoids significantly downregulated detoxification gene expression. In vivo bioassays demonstrated that pectolinarin markedly increased clothianidin susceptibility, achieving a synergistic effect with an LCR50 of 2.87. These results demonstrate that targeting upstream GPCR-mediated regulatory nodes with natural synergists offers a promising and sustainable approach to managing metabolic resistance in agricultural pests.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tudor staphylococcal nuclease promotes Bombyx mori cytoplasmic polyhedrosis virus replication through lipid metabolic reprogramming in silkworms. 都铎葡萄球菌核酸酶通过家蚕脂质代谢重编程促进家蚕细胞质多角体病毒复制。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-20 DOI: 10.1111/1744-7917.70344

Mian Muhammad Awais, Nasir Mehmood, Wensheng Yu, Ayman Asif, Yinong Zhang, Jingchen Sun, Huichao Yan

{"title":"Tudor staphylococcal nuclease promotes Bombyx mori cytoplasmic polyhedrosis virus replication through lipid metabolic reprogramming in silkworms.","authors":"Mian Muhammad Awais, Nasir Mehmood, Wensheng Yu, Ayman Asif, Yinong Zhang, Jingchen Sun, Huichao Yan","doi":"10.1111/1744-7917.70344","DOIUrl":"https://doi.org/10.1111/1744-7917.70344","url":null,"abstract":"Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), a double-stranded RNA reovirus, causes significant losses in sericulture; however, the host factors that support its replication remain poorly understood. Here, we identify Tudor staphylococcal nuclease (TSN) as a critical pro-viral host factor in silkworms. TSN is a multifunctional, evolutionarily conserved protein involved in transcription and RNA processing. BmTSN expression was increased markedly in BmCPV-infected midgut tissues. RNAi-mediated knockdown in BmN cells suppresses viral replication, whereas overexpression enhances it. Mechanistically, BmTSN drives a metabolic shift by enhancing lipid accumulation; knockdown of BmTSN reduces the expression of BmSREBP1 and other lipid-regulatory genes. Disruption of either BmTSN or BmSREBP1 reduces BmCPV replication. In a reciprocal effect, RNAi-mediated knockdown of BmSREBP1 also reduces BmTSN expression. Furthermore, BmSREBP1 transcriptionally activates BmTSN expression by binding to the BmTSN promoter, suggesting a regulatory loop between these factors. Together, these findings reveal a virus-induced BmTSN-BmSREBP1 axis that links host lipid metabolism to BmCPV replication. This work identifies BmTSN as a key host-dependency factor and provides new insights into virus-host interactions in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ventral nerve cord enriched major allergen family gene maintains muscle function under heat stress in Bactrocera dorsalis. 腹侧神经索富集的主要过敏原家族基因维持背小实蝇在热应激下的肌肉功能。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-19 DOI: 10.1111/1744-7917.70342

Jiashuo Li, Ban Chen, Yong Zhang, Luyang Li, Chenfan Duan, Xiaocong Wang, Weiwei Zheng, Hongyu Zhang, Xiaoxue Li

{"title":"A ventral nerve cord enriched major allergen family gene maintains muscle function under heat stress in Bactrocera dorsalis.","authors":"Jiashuo Li, Ban Chen, Yong Zhang, Luyang Li, Chenfan Duan, Xiaocong Wang, Weiwei Zheng, Hongyu Zhang, Xiaoxue Li","doi":"10.1111/1744-7917.70342","DOIUrl":"https://doi.org/10.1111/1744-7917.70342","url":null,"abstract":"Heat stress threatens insect survival by disrupting tissue homeostasis and physiological performance, yet how insects preserve tissue function under high temperature remains poorly understood. Members of the major allergen gene family are widely distributed in insects, but whether and how they contribute to heat stress adaptation remains unclear. Here, using the tropical and subtropical horticultural pest Bactrocera dorsalis, a species capable of surviving temperatures above 40 °C, as a model, we identified a previously uncharacterized heat-responsive gene, matl2, which encodes a secreted lipid-binding protein and serves as a key regulator of muscle function during heat stress. Matl2 protein is secreted and targets the thoracic muscles. Under heat stress, the downregulation of matl2 maintains muscle protein homeostasis, potentially through autophagy and lysosome system, which might involve modulation of the PIP3-Akt signaling pathway. Moreover, Matl2 preserves locomotor performance under heat stress. Together, these findings uncover a lipid-mediated signaling axis that safeguards muscle proteostasis, providing insight into a strategy insects employ to cope with heat stress.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0