Insect Science最新文献_第8页

Transcription factor Nlci is essential for wing dimorphism in the brown planthopper, Nilaparvata lugens. 转录因子Nlci在褐飞虱翅膀二态性中起重要作用。

IF 3 1区农林科学

Insect Science Pub Date : 2025-07-27 DOI: 10.1111/1744-7917.70127

Dong Wen, He Jiang, Zhuangzhuang Qiao, Zewei Lu, Muhua Zhao, Miaomiao Tian, Shaoqin Li, Hongxia Hua

{"title":"Transcription factor Nlci is essential for wing dimorphism in the brown planthopper, Nilaparvata lugens.","authors":"Dong Wen, He Jiang, Zhuangzhuang Qiao, Zewei Lu, Muhua Zhao, Miaomiao Tian, Shaoqin Li, Hongxia Hua","doi":"10.1111/1744-7917.70127","DOIUrl":"https://doi.org/10.1111/1744-7917.70127","url":null,"abstract":"The brown planthopper (BPH) alternatively develops into either macropterous or brachypterous morphs in response to environmental stimuli, enhancing its fitness and dispersal. Despite several key upstream regulator genes involved in wing morphs switching having been identified, the downstream pathways governing wing plasticity remain unclear. Here, we harvested the 4th instar nymphal wing buds from macropterous strain (MS) and brachypterous strain (BS) for transcriptome sequencing. Individually, 3 025 and 3 273 differentially expressed genes (DEGs) were identified in forewing and hindwing buds, respectively, between 2 strains. Functional enrichment analysis revealed that these DEGs were significantly enriched in various categories related to signal transduction and metabolism. Among these, the Hedgehog (Hh) pathway was implicated in environmental information processing and wing development regulating. Knocking down the transcription factor Cubitus interruptus (Nlci) of the Hh pathway significantly reduced the wing size and resulted in malformed wings of both strain adults. Additionally, the expression levels of downstream genes (Nlptc, Nlen, and Nldpp) were inhibited to varying degrees. Furthermore, the expression levels of Nlci and its downstream genes were decreased when injected with double-stranded (ds)NlInR1, but increased in dsNlInR2 treatment. Silencing of Nlci partially antagonized the acceleration effect of dsNlInR2 treatment on wing development, leading to an intermediate wing morph. Our findings expand the regulatory role of the Hh pathway in the wing dimorphism of BPH, providing new insights into the mechanisms underlying wing development.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144730194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of hypomethylation on immune system of Galleria mellonella L. (Lepidoptera: Pyralidae). 低甲基化对黄颡鱼蛾免疫系统的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-22 DOI: 10.1111/1744-7917.70123

Fevzi Uçkan, Rabia Schaeffer, Zülbiye Demirtürk, Ekrem Ergin, Ali Sazci

{"title":"Effects of hypomethylation on immune system of Galleria mellonella L. (Lepidoptera: Pyralidae).","authors":"Fevzi Uçkan, Rabia Schaeffer, Zülbiye Demirtürk, Ekrem Ergin, Ali Sazci","doi":"10.1111/1744-7917.70123","DOIUrl":"https://doi.org/10.1111/1744-7917.70123","url":null,"abstract":"Galleria mellonella is becoming increasingly used as a model organism for studying human pathogen infection disease. Therefore, all factors affecting the level of immune response of G. mellonella should be evaluated. One of these factors is DNA methylation, among the most important epigenetic modifications. Firstly, we investigated the presence of CpG dinucleotides and their methylation status of eggs, last instars, pupae, and adults using HpaII, MspI, and ScrFI methylation-sensitive restriction endonucleases. The gel analysis of the restriction pattern of HpaII, MspI, ScrFI, and MspI+ScrFI on G. mellonella genomic DNA showed that all enzymes produced a wide range of smears. The digestion of DNA samples of egg, pupa, and adult resulted in a smear at higher molecular weight for MspI+ScrFI, and these differences were statistically significant. These results confirm the presence of CpG dinucleotides and methylation at the internal and outer cytosines in different developmental stages of G. mellonella. Presence of global DNA methylation, which was by 3%-5% was investigated in various developmental stages of G. mellonella. Then, different levels of DNA methylation in the last instars of G. mellonella were created with the hypomethylation agent, zebularine (0.25-32 mg/mL), and immunity responses were examined. The most effective doses of zebularine, to create a global hypomethylation status in G. mellonella larvae, were 0.25 and 1 mg/mL. The humoral/cellular immune response of G. mellonella larvae became weak at high hypomethylation. These results present the level of DNA methylation during all developmental stages of G. mellonella and how zebularine treatment affects physiological processes.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maltase 1 regulates DENV2 infection and life history in Aedes aegypti. 马耳他酶1调控埃及伊蚊DENV2感染和生活史。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-22 DOI: 10.1111/1744-7917.70122

Man-Jin Li, Jin Cheng, Ya-Ming Zou, Quan-Chao Liu, Ding Zhu, Ce-Jie Lan

引用次数: 0

Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes. 不可胜选扶桑的遗传结构、扩散途径和向北扩张预测：中国不同景观的20年之旅。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-21 DOI: 10.1111/1744-7917.70133

Jun Huang, Chen-Di Yu, Shi-Zhen Wang, Jian-Dong Bao, Zhi-Tao Jiang, Wan-Ying Dong, Hong-Li Su, Li-Ming Chen, Farman Ullah, Shu-Xing Zhou, Xiao-Wei Li, Jin-Ming Zhang, Zhi-Jun Zhang, Yao-Bin Lu, Juan Zhang

{"title":"Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes.","authors":"Jun Huang, Chen-Di Yu, Shi-Zhen Wang, Jian-Dong Bao, Zhi-Tao Jiang, Wan-Ying Dong, Hong-Li Su, Li-Ming Chen, Farman Ullah, Shu-Xing Zhou, Xiao-Wei Li, Jin-Ming Zhang, Zhi-Jun Zhang, Yao-Bin Lu, Juan Zhang","doi":"10.1111/1744-7917.70133","DOIUrl":"https://doi.org/10.1111/1744-7917.70133","url":null,"abstract":"The red imported fire ant, Solenopsis invicta, has invaded China for over two decades, advancing northwest from Guangdong to the Yangtze River coast at 30.8° N latitude. A decade since the last genetic analysis, this study revisits the species' genetic structure and invasion trajectory amidst intensifying selective pressures. Through mitochondrial DNA resequencing of 96 samples across 6 Chinese provinces, we identified 2 haplotypes (H5 and H22), with H22 predominating in Zhejiang, the northern limit of our study. Phylogenetic analysis suggests a likely invasion source from Guangdong and Fujian, with long-distance dispersal facilitated by the horticultural trade, particularly Cycas revoluta. Local dispersal rates varied significantly, with 40 m/year in northern Zhejiang and 118-186 m/year in Guangdong. A regression model (y = -303.2523 + 10 855.1813/x) forecasts a northern expansion threshold near southern Shandong. However, considering the bridgehead effect, repeated invasions, and climate change, the species' invasive range may extend further north.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transovarial transmission of Wolbachia bacteria via P44/Msp2-IMP2 mediated endocytosis. 通过P44/Msp2-IMP2介导的胞吞作用传播沃尔巴克氏菌。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-18 DOI: 10.1111/1744-7917.70129

Lu Ren, Yan-Jun Men, Xiao-Li Bing, Ary Hoffmann, Xiao-Yue Hong

{"title":"Transovarial transmission of Wolbachia bacteria via P44/Msp2-IMP2 mediated endocytosis.","authors":"Lu Ren, Yan-Jun Men, Xiao-Li Bing, Ary Hoffmann, Xiao-Yue Hong","doi":"10.1111/1744-7917.70129","DOIUrl":"https://doi.org/10.1111/1744-7917.70129","url":null,"abstract":"Wolbachia is a maternally inherited endosymbiont that stably localizes in oocytes of arthropods to ensure successful transovarial transmission. However, the source of Wolbachia in oocytes is unclear. Here, we obtained a Wolbachia strain that is transovarially transmitted with complete fidelity in the agricultural pest spider mite Tetranychus truncatus. Using fluorescence in situ hybridization, we showed that Wolbachia migrate from the digestive cells to the reproductive system as a female develops. When Wolbachia from T. truncatus was transferred to Aedes albopictus cells, we demonstrated that its cell-to-cell spread was regulated by the host's endocytosis system. By assaying the proteome, we identified a bacterial surface protein P44/Msp2 from Wolbachia that interacted with the host's integral membrane protein 2 (IMP2). RNA interference of IMP2 indicated that it facilitated entry of Wolbachia. Our results show that Wolbachia from somatic cells contribute to its transovarial transmission, and that Wolbachia also utilize the surface protein to invade new host cells. This extends our understanding of how Wolbachia is transmitted between cells and facilitates artificial transfection work.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual mechanisms of olfactory plasticity: Transient modulation by phosphatidylcholine and D-chiro-inositol and sustained regulation via the sex peptide-juvenile hormone system. 嗅觉可塑性的双重机制：磷脂酰胆碱和d -肌醇的瞬时调节和性肽-幼年激素系统的持续调节。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70114

Xiaoqing Yao, Chang Luo, Shupei Ai, Fei Cheng, Shuyuan Xing, Guohua Zhong, Xin Yi

{"title":"Dual mechanisms of olfactory plasticity: Transient modulation by phosphatidylcholine and D-chiro-inositol and sustained regulation via the sex peptide-juvenile hormone system.","authors":"Xiaoqing Yao, Chang Luo, Shupei Ai, Fei Cheng, Shuyuan Xing, Guohua Zhong, Xin Yi","doi":"10.1111/1744-7917.70114","DOIUrl":"https://doi.org/10.1111/1744-7917.70114","url":null,"abstract":"Insects exhibit olfactory plasticity to optimize their survival and reproduction, which ensures that their sensory preferences flexibly meets their needs. However, a comprehensive understanding of the sequential regulatory mechanisms driving these sensory adjustments is lacking. Addressing this knowledge gap is crucial for clarifying how the olfactory system of insect flexibly meets their reproductive needs. Using electroantennogram (EAG) and single-sensillum recordings, we found that female Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) displayed distinct olfactory preferences for oviposition cues, host volatiles, and sex pheromones before and after mating. The injection of synthesized sex peptide (SP) or juvenile hormone (JH) into virgin females, as well as the knockdown of SP- or JH-receptors in mated females, reversed the olfactory preferences in both groups of flies. The onset of the effect of JH was relatively gradual, as evidenced by the lack of changes observed during the first 6 h after mating. The rapid changes in antennal sensitivities may involve specific neuromolecules, such as phosphatidylcholine (PC) and D-chiro-inositol (DCI), which were identified through metabolomics screening, and their functions were further validated by microinjection. Our findings reveal a post-mating regulatory mechanism where immediate olfactory adjustments are facilitated by rapid signaling molecules, while long-term sensitivity modifications rely on the gradual action of JH.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing of juvenile hormone-related genes through RNA interference leads to molt failure and high mortality in the spongy moth. 通过RNA干扰使幼体激素相关基因沉默导致海绵蛾蜕皮失败和高死亡率。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70117

Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi

{"title":"Silencing of juvenile hormone-related genes through RNA interference leads to molt failure and high mortality in the spongy moth.","authors":"Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi","doi":"10.1111/1744-7917.70117","DOIUrl":"https://doi.org/10.1111/1744-7917.70117","url":null,"abstract":"Lymantria dispar (spongy moth) is a globally polyphagous and destructive pest, with the larval stage being the main destructive stage. The larvae feed on more than 600 species of plants ranging from oaks to conifers, and they are capable of completely defoliating entire trees, leading to significant ecological and economic losses. Chemical insecticides are commonly used to control them, but these methods are expensive and nonspecific. Therefore, RNA interference (RNAi) is being explored as an alternative strategy to control spongy moth. Juvenile hormones (JHs) play a crucial role in regulating insect growth, diapause, and reproduction, making them potential targets for pest control. This study investigated whether 2 enzymes involved in the JH pathways-JH acid methyltransferase (JHAMT) from the biosynthetic pathway and JH epoxide hydrolase (JHEH) from the metabolic pathway-could be used for RNAi-based control. Ldjhamt showed predominant expression during the larval stages and Ldjheh exhibited high expression throughout development. Both genes had the highest expression in the fat body. RNAi silencing using specific double-stranded RNAs resulted in a significant decrease in the expression level of target genes, as well as a significant reduction in larval survival rate, pupation rate and eclosion rate. Additionally, many larvae and pupae died due to developmental abnormalities, and the adult size of the treated group was smaller than the control group. These findings demonstrate that jhamt and jheh are vital for the growth and development of spongy moths and suggest they could be effective targets for RNAi-based pest control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a dual-loop double-stranded RNA to control small brown planthopper. 双环双链RNA在小褐飞虱控制中的应用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70128

Panpan Ge, Xiangyang Lu, Wan Zhao, Shaohui Wu, Neil Smith, Ling-Ling Gao, Hui-Shan Guo, Ming-Bo Wang, Feng Cui

{"title":"Application of a dual-loop double-stranded RNA to control small brown planthopper.","authors":"Panpan Ge, Xiangyang Lu, Wan Zhao, Shaohui Wu, Neil Smith, Ling-Ling Gao, Hui-Shan Guo, Ming-Bo Wang, Feng Cui","doi":"10.1111/1744-7917.70128","DOIUrl":"https://doi.org/10.1111/1744-7917.70128","url":null,"abstract":"RNA interference, induced by double-stranded RNA (dsRNA), has emerged as a biopesticide for pest control. However, the stability of dsRNA remains a challenge in topical delivery, preventing widespread agricultural application. Here, we tested a dsRNA variant, called loop-ended dsRNA or LedRNA, for controlling a notorious vector insect, the small brown planthopper (SBPH). LedRNA is characterized by having a loop at both ends of the dsRNA stem, resembling a close-circled RNA. Three SBPH target genes, including Flotillin2, which is vital for viral transmission, and trehalose-6-phosphate synthase (TPS) and malate dehydrogenases (MDH), which are essential for insect survival, were tested for gene silencing efficiencies through microinjection, soaking, or oral delivery of the conventional dsRNA, hairpin dsRNA, and LedRNA. All 3 molecules induced effective gene silencing using microinjection, but did not suppress target gene expression through soaking. Notably, LedRNA demonstrated superior efficacy in inducing gene silencing in SBPH through oral feeding compared to the other 2 molecules. Silencing efficiencies ranged from 26% to 39% for Flotillin2, 39% to 51% for TPS, and 45% to 64% for MDH. When SBPH were fed Escherichia coli-synthesized LedRNA targeting these 3 genes, silencing efficiencies ultimately reached 28% to 74%, leading to reduced insect survival and decreased viral transmission in host plant rice. This study highlights the potential of LedRNA as a biopesticide for insect pest control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two transcription factor cascades regulate odorant-binding protein NlOBP8 affecting the recognition of linalool in the brown planthopper Nilaparvata lugens. 两个转录因子级联调节气味结合蛋白NlOBP8，影响褐飞虱对芳樟醇的识别。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70118

Ruilin Zhang, Shuanggang Duan, Sara Taha Abdelkhalek, Ao Liu, Chao Wang, Yi Wei, Ruinan Yang, Manqun Wang

{"title":"Two transcription factor cascades regulate odorant-binding protein NlOBP8 affecting the recognition of linalool in the brown planthopper Nilaparvata lugens.","authors":"Ruilin Zhang, Shuanggang Duan, Sara Taha Abdelkhalek, Ao Liu, Chao Wang, Yi Wei, Ruinan Yang, Manqun Wang","doi":"10.1111/1744-7917.70118","DOIUrl":"https://doi.org/10.1111/1744-7917.70118","url":null,"abstract":"The insect olfactory system is pivotal in mediating chemical-driven behaviors, including host plant selection, mate identification, oviposition site localization, and ecological niche recognition. Although odorant-binding proteins (OBPs) are known to facilitate olfactory signal transduction in insects, the transcriptional regulatory mechanisms governing their expression remain elusive. Here, we identified 2 transcriptional cascades involving the transcription factors (TFs) distal-less (Dll) and aristaless (al) that converge to regulate NlObp8 expression in the brown planthopper (Nilaparvata lugens, BPH) through bioinformatics and transcriptomic analysis. The functional impact of the 2 TFs was validated using the dual-luciferase reporter assays and yeast 1-hybrid assays. RNA interference-mediated knockdown of Nlal significantly downregulated NlObp8 expression and abolished the repellent behavioral response of BPHs to linalool. Furthermore, silencing of NlDll resulted in significant downregulation of Nlal expression. These results revealed that the cascade regulatory mechanism, involving direct al binding and indirect Dll-mediated transcriptional activation, provided molecular targets for disrupting olfactory-driven behaviors in agricultural pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond strain maintenance: Pre-Filter, a viable practice to incorporate desirable traits to genetic sexing strains (GSS) of Ceratitis capitata used in sterile insect technique (SIT) control programs. 在菌种维持之外：预过滤是一种可行的方法，可以将理想的性状纳入到不育昆虫技术（SIT）控制程序中使用的头角杆菌遗传性别菌株（GSS）中。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70091

Edwin Mauricio Ramírez-Santos, Pedro Alfonso Rendón Arana, Lested Darío Rivas-González, Raúl Menéndez Cahueque

{"title":"Beyond strain maintenance: Pre-Filter, a viable practice to incorporate desirable traits to genetic sexing strains (GSS) of Ceratitis capitata used in sterile insect technique (SIT) control programs.","authors":"Edwin Mauricio Ramírez-Santos, Pedro Alfonso Rendón Arana, Lested Darío Rivas-González, Raúl Menéndez Cahueque","doi":"10.1111/1744-7917.70091","DOIUrl":"https://doi.org/10.1111/1744-7917.70091","url":null,"abstract":"The sterile insect technique (SIT) for controlling the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), requires the mass-rearing of males in bio-facilities. This study summarizes the implementation of a Pre-Filter system in the mass-rearing process of C. capitata at the Moscamed program's El Pino facility in Guatemala. The Pre-Filter selection process as presented here was aimed to increase the longevity of the VIENNA 8 D53- strain males released for pest control. Over a 9-year period (2015-2024), the longevity of these males was systemically monitored, and the impact of the Pre-Filter system on mortality, half-life, and survival rate were analyzed. Results indicated a significant reduction in mortality at 48 h, declining from 16.47% in 2017 to 2.56% in 2024. Concurrently, the half-life of males increased from 79.55 h in 2018 to 85.97 h in 2024. The Pre-Filter system shifted the frequency distribution of half-lives toward longer durations, with a peak observed between 76.14 and 86.14 h, whereas populations without the Pre-Filter exhibited shorter half-lives, peaking between 56.14 and 66.14 h. Comparative analyses with historical data (2005-2024) demonstrated that the Pre-Filter system significantly improved the male longevity, compared to pre-implementation periods during which mortality rates were higher and more variable. Additionally, the results revealed a gradual reduction in longevity variability within the population, indicating a more homogeneous longevity among the mass-reared males.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0