Insect Science最新文献_第8页

Foraging Drosophila suzukii larvae exhibit chemotaxis and invade intact, uninfested fruit. 觅食的铃木果蝇幼虫表现出趋化性，入侵完整的、未受侵染的果实。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-19 DOI: 10.1111/1744-7917.70341

Corey Reese, Nabil Killiny, Cesar Rodriguez-Saona, Peter Witzgall, Lukasz L Stelinski

{"title":"Foraging Drosophila suzukii larvae exhibit chemotaxis and invade intact, uninfested fruit.","authors":"Corey Reese, Nabil Killiny, Cesar Rodriguez-Saona, Peter Witzgall, Lukasz L Stelinski","doi":"10.1111/1744-7917.70341","DOIUrl":"https://doi.org/10.1111/1744-7917.70341","url":null,"abstract":"Spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), is a destructive pest of ripening berries and stone fruits, where larval feeding rapidly renders fruit unmarketable. Larvae are widely assumed to remain inside their natal fruit until pupation, limiting exposure to external management tactics. Here, we challenge this paradigm by demonstrating that D. suzukii larvae exhibit complex chemotactic capabilities and dynamic interactions with host fruit throughout development. Third-instar larvae responded in a dose-dependent manner to fermentation-associated volatiles and discriminated between conspecific and heterospecific larval developmental cues, indicating the capacity for substrate assessment. Complementary to these chemotaxis assays, direct observations across multiple hosts revealed frequent larval emergence from fruit, including posterior spiracle, head, and whole-body exposure, as well as a previously undocumented behavior: secondary infestation of neighboring, uninfested fruit. Secondary infestation occurred across all hosts tested and throughout larval development, with cumulative movement into fresh berries exceeding 60% in some fruit types. Host species and larval density modulated the frequency and timing of emergence, and inter-fruit dispersal, highlighting the influence of natal environment on larval behavior. Together, these findings suggest that D. suzukii larvae are not confined to a single fruit but can interact repeatedly with fruit surfaces and move into neighboring hosts. This revised understanding of D. suzukii larval ecology exposes new vulnerabilities in the life cycle and has possible implications for the development of management strategies targeting immature stages.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symbiont-mediated insecticide resistance in insect hosts: Mechanisms and integrated management prospects. 昆虫寄主共生体介导的杀虫剂抗性：机制和综合治理前景。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-19 DOI: 10.1111/1744-7917.70340

Jiayun Jiang, Yingchun Cao, Niguse Bekele Dirbaba, Yuxian Xia, Jiaqin Xie

{"title":"Symbiont-mediated insecticide resistance in insect hosts: Mechanisms and integrated management prospects.","authors":"Jiayun Jiang, Yingchun Cao, Niguse Bekele Dirbaba, Yuxian Xia, Jiaqin Xie","doi":"10.1111/1744-7917.70340","DOIUrl":"https://doi.org/10.1111/1744-7917.70340","url":null,"abstract":"The rapid escalation of insecticide resistance poses a profound threat to global food security and public health. While chemical resistance mechanisms are well characterized, the functional contribution of symbiotic microorganisms has only recently gained prominence. However, the intricate molecular cross-talk between insect symbionts and host detoxification pathways remains poorly defined, constraining our capacity to leverage these interactions for resistance management. This review synthesizes current findings on how endosymbionts and gut microbiota modulate insecticide resistance across diverse pest lineages. We critically evaluate evidence from multiple systems, underscoring the inherent complexity and context-dependency of symbiont-mediated effects. A pivotal insight emerging from this synthesis is the dual role of specific symbionts, which may either augment or attenuate host resistance contingent upon environmental stressors and host genetic architecture. Building on these foundations, we propose a \"holobiont\" that conceptualizes the insect host and its associated microbiota as a unified functional entity in the evolution of resistance. This framework identifies discrete molecular interfaces and symbiont taxa as promising candidates for intervention. By integrating mechanistic insights with applied objectives, this review delineates a strategic roadmap for the development of sustainable, next-generation tools to mitigate the spread of insecticide resistance.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Humble beginnings of the Australian giant wood moth (Endoxyla cinereus): A century-old mystery solved. 澳大利亚巨型木蛾（Endoxyla cinereus）的卑微起源：一个世纪的谜团解开了。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-18 DOI: 10.1111/1744-7917.70343

Jessa H Thurman, Lyn G Cook, James Hereward, Andrew J Maynard, Geoff B Monteith

引用次数: 0

A potent EF1A promoter and a minimal U6 promoter enable highly efficient CRISPR genome editing in Aedes aegypti. 一个有效的EF1A启动子和一个最小的U6启动子能够在埃及伊蚊中实现高效的CRISPR基因组编辑。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-17 DOI: 10.1111/1744-7917.70338

Xiaolin Yang, Houming Ren, Meng Huang, Wenzhuo Zhao, Yujiao Han, Yuqi Lou, Shiping Liu

{"title":"A potent EF1A promoter and a minimal U6 promoter enable highly efficient CRISPR genome editing in Aedes aegypti.","authors":"Xiaolin Yang, Houming Ren, Meng Huang, Wenzhuo Zhao, Yujiao Han, Yuqi Lou, Shiping Liu","doi":"10.1111/1744-7917.70338","DOIUrl":"https://doi.org/10.1111/1744-7917.70338","url":null,"abstract":"CRISPR/Cas9 has become a key tool for functional genomics and genetic control strategies in mosquitoes, with its efficiency highly dependent on precise spatiotemporal regulation of Cas9 expression. While germline-specific promoters help reduce resistance and improve drive efficiency, constitutive promoters confer higher editing efficiency due to their ubiquitous and sustained expression, making them more suitable for gene functional analysis and population-specific suppression. However, the scarcity of strong constitutive promoters in Aedes aegypti (Ae. aegypti) has limited the development of highly efficient editing systems. In this study, we cloned the promoter of the housekeeping gene eukaryotic translation elongation factor 1α (EF1Α, AAEL017096) and confirmed its transcriptional activity. RT-qPCR analysis revealed that the EF1Α promoter (EF1Αp) directed significantly higher Cas9 expression in mosquito ovaries and testes than did the germline-specific Exu promoter (Exup). Crossing EF1Ap-Cas9 transgenic lines with various gRNA-expressing lines achieved efficient editing of the marker gene white, the functional gene PNP, and the microRNA precursor miR-1174, with all double-positive progeny displaying expected loss-of-function phenotypes. To overcome vector capacity limitations, we truncated the U6 promoter. A truncated U6 promoter of only 235 bp (U6p235) supported editing efficiency comparable to the full-length 964 bp version. When U6p235-white gRNA lines were crossed with EF1Ap-Cas9 lines, all double-positive offspring exhibited a white-eye phenotype, confirming successful white gene disruption. Together, this work provides an efficient constitutive promoter and a compact U6p, expanding the genetic toolkit for Ae. aegypti and supporting advanced functional studies and multi-target control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanocarrier-mediated delivery of dsRNA targeting the ecdysone receptor enables non-invasive RNAi control of ticks. 纳米载体介导的dsRNA靶向脱皮激素受体的递送能够实现对蜱虫的非侵入性RNAi控制。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-12 DOI: 10.1111/1744-7917.70339

Qian Yao, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Yanan Wang, Jinlin Zhou

{"title":"Nanocarrier-mediated delivery of dsRNA targeting the ecdysone receptor enables non-invasive RNAi control of ticks.","authors":"Qian Yao, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Yanan Wang, Jinlin Zhou","doi":"10.1111/1744-7917.70339","DOIUrl":"10.1111/1744-7917.70339","url":null,"abstract":"Ticks are ectoparasites that can transmit zoonotic pathogens, causing significant economic losses worldwide. RNA interference (RNAi) offers a gene-specific alternative to chemical acaricides; however, its application is limited by double-stranded RNA (dsRNA) instability and poor cuticle penetration. Using Haemaphysalis longicornis (H. longicornis) as a model, we identified the ecdysone receptor gene (ECR) as an effective RNAi target. Microinjection of dsECR completely abolished engorgement rate (0%) and reduced body weight by ∼90%. To enable practical delivery, three nanocarrier systems-chitosan (CS), star polycation (SPc), and disulfide-crosslinked chitosan (CS-ss) were developed to improve the stability and uptake of dsRNA. All three nanocarrier systems enhanced dsRNA resistance to ribonuclease (RNase) degradation while exhibiting distinct physicochemical properties: CS-ss formed the smallest nanoparticles (∼175 nm), whereas SPc showed superior surface wettability. All nanocarrier-dsRNA complexes achieved comparable RNAi efficiency under microinjection. In contrast, immersion delivery of naked dsECR was ineffective, whereas SPc-dsECR and CS-ss-dsECR enabled significant ECR silencing, reduced engorgement rate, and decreased body weight. Similar RNAi efficiency levels were validated in Hyalomma asiaticum, supporting the cross-species applicability of this strategy. Notably, SPc-dsRNA exhibited lower contact angles on the tick cuticle, indicating enhanced spreading and suggesting improved penetration efficiency. Collectively, the results of this study demonstrate that nanocarrier encapsulation is effective for immersion-based RNAi control of ticks. SPc provides superior dispersion and surface interaction for consistent delivery, while CS-ss offers the potential for redox-responsive release. The findings establish a scalable and non-invasive RNAi strategy for sustainable tick control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148724412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host age-mediated effects on fitness traits of five Anastatus parasitoids: Implications for optimizing biological control of Riptortus pedestris. 寄主年龄对5种拟寄生蜂适合度性状的影响：对步行Riptortus生物防治优化的启示

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-10 DOI: 10.1111/1744-7917.70333

Cheng-Chao Hu, Yong-Ming Chen, Ying Zhang, Haneef Tariq, Yi-Ting Li, Asad Ali, Adeney de F Bueno, Lian-Sheng Zang

{"title":"Host age-mediated effects on fitness traits of five Anastatus parasitoids: Implications for optimizing biological control of Riptortus pedestris.","authors":"Cheng-Chao Hu, Yong-Ming Chen, Ying Zhang, Haneef Tariq, Yi-Ting Li, Asad Ali, Adeney de F Bueno, Lian-Sheng Zang","doi":"10.1111/1744-7917.70333","DOIUrl":"https://doi.org/10.1111/1744-7917.70333","url":null,"abstract":"Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) is a widely distributed polyphagous pest, associated with soybean stay-green syndrome, causing substantial yield losses across Asia. Continued reliance on chemical control remains problematic, highlighting the need for sustainable biological alternatives. In this study, we comparatively evaluated the parasitism performance, host-feeding behavior, developmental traits, and host-age preference of five egg parasitoids of the genus Anastatus (A. dexingensis, A. fulloi, A. gansuensis, A. japonicus, and A. shichengensis) on R. pedestris eggs of different ages (0-, 2-, 4-, and 6-d-old). All five parasitoid species successfully completed development on R. pedestris eggs, with offspring emergence rates ranging from 82% to 100%. Parasitism and host-feeding were strongly influenced by host egg age, with all species showing a clear preference for 0- to 2-d-old. Anastatus dexingensis and A. japonicus parasitized the highest number of host eggs (13.5 and 12.3 eggs/24 h, respectively), whereas the thelytokous A. gansuensis exhibited the strongest host-feeding activity (3.8 eggs/24 h). In contrast, A. fulloi and A. shichengensis produced no female progeny, whereas A. dexingensis and A. japonicus produced less than 20% female offspring. Developmental duration generally increased with host age, indicating that older host eggs may be less suitable for parasitoid development. Overall, A. dexingensis and A. japonicus appear well suited for short-term suppression of R. pedestris through inundative releases, whereas the thelytokous A. gansuensis shows strong potential for long-term, sustainable population regulation, making it promising for augmentative biocontrol. These findings provide a scientific basis for selecting effective Anastatus spp. and optimizing egg-stage biocontrol strategies against R. pedestris.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNA bmo_circ_0000157 facilitates microsporidian proliferation via an miR-281-5p-mediated regulatory axis in Bombyx mori. 环状RNA bmo_circ_0000157通过mir -281-5p介导的调控轴促进家蚕微孢子虫增殖。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-03 DOI: 10.1111/1744-7917.70330

Bingyu Guo, Ying Ma, Pengcheng Zhang, Yunxiang Jia, Peng Chen, Zhanqi Dong, Minhui Pan

{"title":"Circular RNA bmo_circ_0000157 facilitates microsporidian proliferation via an miR-281-5p-mediated regulatory axis in Bombyx mori.","authors":"Bingyu Guo, Ying Ma, Pengcheng Zhang, Yunxiang Jia, Peng Chen, Zhanqi Dong, Minhui Pan","doi":"10.1111/1744-7917.70330","DOIUrl":"https://doi.org/10.1111/1744-7917.70330","url":null,"abstract":"Microsporidia are unicellular eukaryotic obligate intracellular parasites that primarily infect vertebrates, invertebrates, and some protists. However, our understanding of microsporidian-host interaction mechanisms remains insufficient, especially the mechanisms underlying the noncoding RNA response to microsporidian infection. To systematically explore the characteristics of the host noncoding RNA response to microsporidian infection, we used whole-transcriptome sequencing technology to identify noncoding RNAs in the Bombyx mori midgut at different time points after Nosema bombycis (N. bombycis) infection. We identified 51 novel microRNAs (miRNAs) and 269 novel circular RNAs (circRNAs) in the Bombyx mori midgut and constructed a differentially expressed (DE)-circRNA-miRNA regulatory interaction network. Furthermore, our qRT-PCR screening revealed that bmo_circ_0000157 (circ_0000157) facilitates N. bombycis proliferation. Ultimately, dual-luciferase reporter and cell-level rescue assays confirmed that circ_0000157 functions as a molecular sponge for bmo-miR-281-5p (miR-281-5p), thereby regulating N. bombycis proliferation. Overall, our results indicate that the novel regulatory target circ_0000157 facilitates N. bombycis proliferation by modulating miR-281-5p expression, thereby altering its effects on downstream target genes. This study expands our understanding of circRNA biological functions and provides an important foundation for elucidating N. bombycis infection mechanisms.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early detection and tracking of wood borers using improved environmental DNA aggregation and TaqMan quantitative PCR approaches in forests. 利用改进的环境DNA聚集和TaqMan定量PCR方法在森林中早期检测和跟踪木蛀虫。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-01 Epub Date: 2025-05-26 DOI: 10.1111/1744-7917.70075

Jingyu Qi, Xiaomeng Gao, Junke Nan, Agbessenou Ayaovi, Mengqin Zhao, Jiangbin Fan, Hong He

{"title":"Early detection and tracking of wood borers using improved environmental DNA aggregation and TaqMan quantitative PCR approaches in forests.","authors":"Jingyu Qi, Xiaomeng Gao, Junke Nan, Agbessenou Ayaovi, Mengqin Zhao, Jiangbin Fan, Hong He","doi":"10.1111/1744-7917.70075","DOIUrl":"10.1111/1744-7917.70075","url":null,"abstract":"The increased number of invasive harmful organisms has exacerbated ecosystem damage. Early monitoring and timely control measures are urgently needed. Although environmental DNA (eDNA) application for monitoring aquatic organisms is well-established, its use in terrestrial monitoring remains limited, particularly for cryptic wood-borer pests like Monochamus alternatus, the primary vector of pine wilt disease. We developed and validated quantitative real-time polymerase chain reaction detection assays using TaqMan probes, targeting conserved regions of the ribosomal DNA (rDNA) internal transcribed spacer 1 and D2-D3 expansion segments of the 28S rDNA. eDNA surveys were conducted using the optimization of sample collection methods, effectively tracking M. alternatus in both laboratory and field environments. Laboratory tests revealed M. alternatus presence could be detected in emergence holes over 35 d, as well as in fresh oviposition scars from 1 to 7 d. The concentration of eDNA significantly decreased with the prolongation of sample storage time. Additionally, 3 novel eDNA aggregation approaches were developed to enhance detection sensitivity of wood-borer pests in forests: (i) rinsing aggregation, which gathers eDNA from the vicinity of emergence holes; (ii) wet cotton ball dipping, mainly used to collect residual eDNA in the oviposition scars and emergence holes; and (iii) natural predators, where M. alternatus can be identified by collecting ants on the surface of pine trunks. The results demonstrate the effectiveness and high sensitivity of the eDNA approach for M. alternatus monitoring. These findings provide valuable insights for early detection efforts and can serve as a reference for similar eDNA surveys targeting other wood-boring pests.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1675-1690"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding biological control function and trophic interaction dynamics of an artificially released predatory bug by DNA metabarcoding. 利用DNA元条形码技术了解人工释放捕食性昆虫的生物防治功能和营养相互作用动力学。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-01 Epub Date: 2025-05-25 DOI: 10.1111/1744-7917.70074

Weidong Huang, Suyi Chen, Zihua Zhao, Chunyan Yang, Anxian Shi, Hu Li, Yuange Duan, Fan Song, Hailin Yang, Wanzhi Cai

{"title":"Understanding biological control function and trophic interaction dynamics of an artificially released predatory bug by DNA metabarcoding.","authors":"Weidong Huang, Suyi Chen, Zihua Zhao, Chunyan Yang, Anxian Shi, Hu Li, Yuange Duan, Fan Song, Hailin Yang, Wanzhi Cai","doi":"10.1111/1744-7917.70074","DOIUrl":"10.1111/1744-7917.70074","url":null,"abstract":"Understanding the dietary habits of generalist invertebrate predators in agroecosystems helps improve ecosystem programs, particularly for integrated pest management. Augmentation biological control, one of three widely accepted biological pest control methods, involves releasing a large number of natural enemies to quickly reduce damaging pest populations. However, the effectiveness of these natural enemies against target pests and their effects on nontarget pests in agricultural landscapes remain unknown. This study aimed to investigate the dietary composition of Sycanus bifidus, a generalist predator of the assassin bug that is artificially released into citrus orchards, and uncover its trophic structure across various pest species using metabarcoding-based molecular gut content analysis. We detected 30 different prey taxa from 8 orders and 19 families in 108 samples of S. bifidus recaptured over 22 d. Lepidoptera (9) made up 30% of their diet, followed by Coleoptera (5), Hemiptera (5), and Hymenoptera (5). Euproctis varians, a major lepidopteran citrus pest, was frequently found in the gut of S. bifidus with a high incidence and relative read abundance, suggesting its potential as an effective biological control agent for E. varians in citrus crops. Additionally, our findings showed that the choice of primer pair critically influenced the efficiency of diet detection, with the Fwh primer set being ideal for targeting highly degraded DNA in insect guts. This study demonstrated that metabarcoding is a valuable tool for studying predator diets, reconstructing trophic interactions in agricultural landscapes, optimizing pest management strategies, and identifying effective biological control agents.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1633-1648"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Camptothecin weakens the mechanical properties of insect cuticle by inhibiting tanning. 喜树碱通过抑制鞣制而削弱了昆虫角质层的力学性能。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-08-01 Epub Date: 2025-05-01 DOI: 10.1111/1744-7917.70065

Yi Ding, Yuntian Cui, Huijie Xie, Jinhui Liang, Huan Liu, Fenghou Yuan, Tian Liu

{"title":"Camptothecin weakens the mechanical properties of insect cuticle by inhibiting tanning.","authors":"Yi Ding, Yuntian Cui, Huijie Xie, Jinhui Liang, Huan Liu, Fenghou Yuan, Tian Liu","doi":"10.1111/1744-7917.70065","DOIUrl":"10.1111/1744-7917.70065","url":null,"abstract":"The mode of action of botanical pesticides is often complex. In this study, camptothecin (CPT), a plant-derived compound, was found to alter the mechanical properties of the cuticle in the lepidopteran pest, Ostrinia furnacalis. CPT treatment resulted in a thinner, distorted cuticle with significantly reduced pigmentation. Fourier transformed infrared spectroscopy indicated decreased tyrosine-mediated crosslinking and melanin formation, while metabolomics showed a significant reduction in N-acetyldopamine content, with no reduction in dopamine or related precursors. Despite a slight increase in laccase 2 transcript levels, laccase activity was significantly reduced in CPT-fed larvae, suggesting that CPT may inhibit laccase translation or zymogen activation. This reduction in laccase activity led to impaired sclerotization and pigmentation, producing a disordered cuticle with weakened mechanical properties. These findings provide new insights into the mode of action of botanical pesticides and their potential to regulate insect cuticle formation.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1513-1524"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0