Insect Science最新文献_第9页

An integrative strategy used by the aphid Uroleucon formosanum to counter host sesquiterpene lactone defense: Insights from combined genomic and transcriptomic analysis. 蚜虫 Uroleucon formosanum 对抗寄主倍半萜内酯防御的综合策略：基因组和转录组联合分析的启示。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-30 DOI: 10.1111/1744-7917.13452

Jing-Bo Li, Li-Yun Jiang, Ge-Xia Qiao, Jing Chen

{"title":"An integrative strategy used by the aphid Uroleucon formosanum to counter host sesquiterpene lactone defense: Insights from combined genomic and transcriptomic analysis.","authors":"Jing-Bo Li, Li-Yun Jiang, Ge-Xia Qiao, Jing Chen","doi":"10.1111/1744-7917.13452","DOIUrl":"https://doi.org/10.1111/1744-7917.13452","url":null,"abstract":"Insect herbivores adapt and develop strategies to counteract plant chemical defenses. The aphid Uroleucon formosanum is a serious sap-sucking pest that infests lettuces containing toxic sesquiterpene lactones (STLs). Herein, we employed a combination of genome sequencing and RNA-seq transcriptome profiling to understand the mechanisms underlying phytotoxin tolerance in U. formosanum. We generated the first chromosome-level genome assembly for U. formosanum, with a total size of 453.26 Mb and a scaffold N50 of 33.22 Mb. Comparative genomic analyses revealed an enrichment of signals for positive selection and gene family expansion in immune-related pathways. Specifically, the expanded set of heat shock protein 70 (HSP70) genes showed upregulation after treatment with lactucin, suggesting that they may play a role in the immune response against STLs. The expression of takeout-like genes and cuticle-associated genes was also significantly increased in the lactucin-treated samples. Additionally, 53 cytochrome P450 monooxygenase, 30 carboxylesterase, 19 glutathione S-transferase, 32 uridine diphosphate glycosyltransferase and 63 ATP-binding cassette (ABC) transporter genes were identified in the U. formosanum genome. CYP4C1, CYP6A13 and 7 ABC genes were strongly upregulated in response to lactucin treatment, indicating the involvement of detoxifying enzymes in the tolerance of U. formosanum to STLs. Our findings suggest that the cuticle barrier, immune response and enzyme-mediated metabolic detoxification jointly enhance the tolerance of U. formosanum to phytotoxins and promote its adaptation to host plants. This study presents a valuable genomic resource and provides insights into insect adaptation to plant chemical challenges and future technological developments for pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of alarm pheromone components of the southern giant Asian hornet, Vespa soror, a major pest of honey bees. 鉴定蜜蜂的主要害虫--亚洲南方大黄蜂（Vespa soror）的报警信息素成分。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-24 DOI: 10.1111/1744-7917.13448

Shihao Dong, Aili Sun, Tao Lin, Jianjun Li, Gaoying Gu, James C Nieh, Ken Tan

{"title":"Identification of alarm pheromone components of the southern giant Asian hornet, Vespa soror, a major pest of honey bees.","authors":"Shihao Dong, Aili Sun, Tao Lin, Jianjun Li, Gaoying Gu, James C Nieh, Ken Tan","doi":"10.1111/1744-7917.13448","DOIUrl":"https://doi.org/10.1111/1744-7917.13448","url":null,"abstract":"The rise of biological invasions threatens biodiversity and food security, with the vespid family, including Vespa soror, being of particular concern. Our study focused on the alarm pheromone components of V. soror. By using gas chromatography-mass spectrometry (GC-MS) chemical analyses, electroantennograms, and field bioassays, we identified 5 compounds-2-pentanol, 3-methyl-1-butanol, 2-heptanol, 2-nonanol (2-N), and isopentyl acetate (IPA)-in hornet sting venom that elicited defensive behavior from hornets. IPA and 2-N also serve as alarm pheromone components in multiple honey bee species that are important prey for V. soror. This shared chemical signaling may allow cross-detection by each species on the other's alarm cues. While it should be advantageous for bees to detect V. soror alarm pheromone, the benefits to V. soror of using IPA and 2-N are unclear. V. soror may manipulate bee behavior, potentially distracting defenders, because they mark victim bee colonies by rubbing their abdomens, which contain their sting glands, at bee hive entrances. Our findings pose new evolutionary questions about the role of manipulation in the arms races.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient CRISPR-mediated genome editing can be initiated by embryonic injection but not by ovarian delivery in the beetle Tribolium castaneum. 在甲虫Tribolium castaneum中，胚胎注射可以启动高效的CRISPR介导的基因组编辑，而卵巢注射则不行。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-20 DOI: 10.1111/1744-7917.13447

Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Xing-Ce Wang, Tong-Xian Liu, Hong-Gang Tian

{"title":"Efficient CRISPR-mediated genome editing can be initiated by embryonic injection but not by ovarian delivery in the beetle Tribolium castaneum.","authors":"Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Xing-Ce Wang, Tong-Xian Liu, Hong-Gang Tian","doi":"10.1111/1744-7917.13447","DOIUrl":"https://doi.org/10.1111/1744-7917.13447","url":null,"abstract":"The clustered regularly interspaced small palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9)-mediated gene editing technology has revolutionized the study of fundamental biological questions in various insects. Diverse approaches have been developed to deliver the single-guide RNA (sgRNA) and Cas9 to the nucleus of insect embryos or oocytes to achieve gene editing, including the predominant embryonic injection methods and alternative protocols through parental ovary delivery. However, a systematic comparative study of these approaches is limited, especially within a given insect. Here, we focused on revealing the detailed differences in CRISPR/Cas9-mediated gene editing between the embryo and ovary delivery methods in the beetle Tribolium castaneum, using the cardinal and tyrosine hydroxylase (TH) as reporter genes. We demonstrated that both genes could be efficiently edited by delivering Cas9/sgRNA ribonucleoproteins to the embryos by microinjection, leading to the mutant phenotypes and indels in the target gene sites. Next, the Cas9/sgRNA complex, coupled with a nanocarrier called Branched Amphiphilic Peptide Capsules (BAPC), were delivered to the ovaries of parental females to examine the efficacy of BAPC-mediated gene editing. Although we observed that a small number of beetles' progeny targeting the cardinal exhibited the expected white-eye phenotype, unexpectedly, no target DNA indels were found following subsequent sequencing analysis. In addition, we adopted a novel approach termed \"direct parental\" CRISPR (DIPA-CRISPR). However, we still failed to find gene-editing events in the cardinal or TH gene-targeted insects. Our results indicate that the conventional embryonic injection of CRISPR is an effective method to initiate genome editing in T. castaneum. However, it is inefficient by the parental ovary delivery approach.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and function of microRNAs in hemipteran pests: A review 半翅目害虫体内微RNA的鉴定和功能：综述

IF 4 1区农林科学

Insect Science Pub Date : 2024-09-18 DOI: 10.1111/1744-7917.13449

Yipeng Ren, Wenhao Dong, Juhong Chen, Huaijun Xue, Wenjun Bu

{"title":"Identification and function of microRNAs in hemipteran pests: A review","authors":"Yipeng Ren, Wenhao Dong, Juhong Chen, Huaijun Xue, Wenjun Bu","doi":"10.1111/1744-7917.13449","DOIUrl":"https://doi.org/10.1111/1744-7917.13449","url":null,"abstract":"Hemiptera is one of the most significant orders of insect pests, including whiteflies, true bugs, aphids, planthoppers, psyllids, and so forth, which have led to substantial economic losses in agricultural industries and have significantly affected food yields through their ability to suck the phloem sap of crops and transmit numerous bacterial and viral pathogens. Therefore, explorations of pest‐specific, eco‐friendly and easy‐to‐adopt technologies for hemipteran pest control are urgently needed. To the best of our knowledge, microRNAs (miRNAs), which are endogenous non‐coding small RNAs approximately 22 nucleotides in length, are involved in regulating gene expression via the direct recognition and binding of the 3′‐untranslated region (3′‐UTR) of target messenger RNAs (mRNAs) or by acting as a center of a competitive endogenous RNA (ceRNA) network at the post‐transcriptional level. This review systematically outlines the characterization and functional investigation of the miRNA biogenesis pathway in hemipteran pests, such as whiteflies, true bugs, aphids and planthoppers. In addition, we explored the results of small RNA sequencing and functional observations of miRNAs in these pests, and the results suggest that the numerous miRNAs obtained and annotated via high‐throughput sequencing technology and bioinformatic analyses contribute to molting development, fitness, wing polyphenism, symbiont interactions and insecticide resistance in hemipteran pests. Finally, we summarize current advances and propose a framework for future research to extend the current data and address potential limitations in the investigation and application of hemipteran miRNAs.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":"17 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori. BmEL-2通过调控BmAGPATγ和BmFAF2的表达来促进桑蚕体内甘油三酯的代谢。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-15 DOI: 10.1111/1744-7917.13446

Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun

{"title":"BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori.","authors":"Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun","doi":"10.1111/1744-7917.13446","DOIUrl":"https://doi.org/10.1111/1744-7917.13446","url":null,"abstract":"Bombyx mori ELAV-like-1 (BmEL-1) and B. mori ELAV-like-2 (BmEL-2) are 2 members of the ELAV-like family of RNA-binding proteins. Mutations in Bmel-1 and Bmel-2 resulted in 5.8% and 28.5% decreases in larval weight on the 3rd day of the 5th instar larva (L5D3), respectively. Triglycerides (TG) are the most important energy resource and are the main component of neutral fat (NF) in animals. To investigate the role of Bmelav-like genes in the synthesis and decomposition of TG, transcriptomic, and metabolic analyses were performed on the whole bodies on the 1st day of the 2nd instar larvae (L2D1) and on fat bodies on L5D3 of Bmel-1- and Bmel-2- mutants, respectively. As compared with the control silkworm, differentially expressed genes generated in both mutants were mainly enriched in lysine degradation, fatty acid (FA) metabolism, and unsaturated FAs biosynthesis. The diglyceride and phosphatide contents were significantly lower in Bmel-1- and Bmel-2- fat bodies than those of the control group. Consistently, the NF content of both mutants' fat bodies were reduced by 50% and 60%, respectively. BmEL-2 positively regulates BmAGPATγ (B. mori 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, LOC101741736) and BmFaF2 (B. mori fatty acid synthetase-associated factor 2, LOC101739090) expression by binding to the specific regions of their 3' untranslated regions in BmN cells. This study suggests that BmEL-2 may be an important regulator of BmAGPATγ and BmFAF2 expression and thereby participates in TG metabolism in the silkworm fat body.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytoplasma infection renders cranberries more susceptible to above- and belowground insect herbivores. 植原体感染使蔓越莓更容易受到地上和地下昆虫食草动物的侵害。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-15 DOI: 10.1111/1744-7917.13444

Cesar Rodriguez-Saona, Paolo Salazar-Mendoza, Robert Holdcraft, James Polashock

{"title":"Phytoplasma infection renders cranberries more susceptible to above- and belowground insect herbivores.","authors":"Cesar Rodriguez-Saona, Paolo Salazar-Mendoza, Robert Holdcraft, James Polashock","doi":"10.1111/1744-7917.13444","DOIUrl":"https://doi.org/10.1111/1744-7917.13444","url":null,"abstract":"While phytoplasma infections in plants are known to affect their interactions with aboveground herbivores, the impact of different genotypes on these infections and their effects on belowground herbivores remains largely unexplored. In cranberry (Vaccinium macrocarpon), infection by the phytoplasma Candidatus Phytoplasma sp. subgroup 16SrIII-Y leads to false blossom disease. This study investigates whether cranberry infection by this phytoplasma affects the performance and feeding behavior of a foliar feeder (spongy moth, Lymantria dispar) and a root feeder (oriental beetle, Anomala orientalis). Using phytoplasma-infected and uninfected cranberries of two genotypes (\"Ben Lear\" and \"Crimson Queen\"), the survival, growth and consumption of L. dispar and A. orientalis larvae were measured. To assess the effects on plant morphological and chemical traits, we also examined the impact of phytoplasma infection on shoot and root growth, carbon and nitrogen content, and the levels of defensive compounds such as proanthocyanidins (PACs). Results indicate that larvae of L. dispar and A. orientalis generally showed larger size and more efficient tissue consumption on infected plants, with these effects varying by cranberry genotype, possibly due to differences in phytoplasma titer. Phytoplasma infection was associated with stunted growth, elevated nitrogen content, and lower PAC levels in both shoots and roots of infected cranberry plants compared to uninfected ones. These findings indicate that phytoplasma infection potentially manipulates plant chemical composition by increasing nutrient levels and decreasing defensive compounds, enhancing herbivore performance both above and belowground. This study sheds light on the intricate interplay among plants, phytoplasma infection, and insect herbivore communities.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR‐927 regulates insect wing development by targeting the Hippo pathway miR-927 通过靶向 Hippo 通路调控昆虫翅膀的发育

IF 4 1区农林科学

Insect Science Pub Date : 2024-09-10 DOI: 10.1111/1744-7917.13445

Xuan Yu, Bing Sun, Xuequan Gao, Qingxin Liu, Zizhang Zhou, Yunhe Zhao

{"title":"miR‐927 regulates insect wing development by targeting the Hippo pathway","authors":"Xuan Yu, Bing Sun, Xuequan Gao, Qingxin Liu, Zizhang Zhou, Yunhe Zhao","doi":"10.1111/1744-7917.13445","DOIUrl":"https://doi.org/10.1111/1744-7917.13445","url":null,"abstract":"How organ size is determined is a fundamental question in life sciences. Recent studies have highlighted the importance of the Hippo pathway in regulating organ size. This pathway controls cell proliferation and cell death to maintain the proper number of cells. The activity of the Hippo pathway is tightly fine‐tuned through various post‐translational modifications, such as phosphorylation and ubiquitination. Here, we discover that miR‐927 is a novel regulator of wing size. Overexpression of miR‐927 decreases wing size, which can be rescued by co‐expressing miR‐927‐sponge. Next, we show that miR‐927 stimulates apoptosis and suppresses the expression of Drosophila inhibitor of apoptosis protein 1, a well‐known target gene of the Hippo pathway. Genetic epistatic analyses position miR‐927 upstream of Yorkie (Yki) to modulate the Hippo pathway. In addition, there is a matching miR‐927 seed site in the yki 3′ untranslated region (3′‐UTR), and we demonstrate that yki 3′‐UTR is the direct target of miR‐927. Ultimately, our study reveals that the targeting of yki by miR‐927 to regulate the Hippo pathway is conserved in Helicoverpa armigera. Administration of miR‐927 via star polycation (SPc) nanocarrier effectively inhibits wing development in H. armigera. Taken together, our findings uncover a novel mechanism by which Yki is silenced at the post‐transcriptional level by miR‐927, and provide a new perspective on pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":"4 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The beetle's structural protein CPCFC making elytra tough and rigid. 甲虫的结构蛋白 CPCFC 使蜕皮坚硬无比。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-05 DOI: 10.1111/1744-7917.13443

Han Bao, Yuantao Liu, Yanwei Duan, Lei Chen, Qing Yang

{"title":"The beetle's structural protein CPCFC making elytra tough and rigid.","authors":"Han Bao, Yuantao Liu, Yanwei Duan, Lei Chen, Qing Yang","doi":"10.1111/1744-7917.13443","DOIUrl":"https://doi.org/10.1111/1744-7917.13443","url":null,"abstract":"The insect cuticle, which serves as both a protective barrier and an efficient lever system for locomotion, is an extracellular matrix primarily composed of chitin and protein. The cuticle protein CPCFC characterized by a \"CFC\" motif containing 2 Cys split by the insertion of 5 residues is distributed across most insect species and specifically localized in the hard part of the cuticle. However, their physiological function is not fully understood. Here, we report 2 CPCFC proteins, TcCPCFC1 and TcCPCFC2, derived from the Coleopteran insect Tribolium castaneum. We revealed that TcCPCFC1 and TcCPCFC2 were predominantly expressed during the larval and adult stages of T. castaneum, respectively. The transcription downregulation of TcCPCFC1 significantly decreased the modulus and toughness of the elytral cuticle. We found that TcCPCFC proteins have high binding affinity to chitin. We cloned and produced recombinant TcCPCFC proteins and demonstrated that the addition of TcCPCFC proteins to chitin hydrogel greatly enhanced the hydrogel's modulus and toughness by forming denser chitin fibrous networks. Our findings reveal the functional role of CPCFC proteins in enhancing mechanical properties of insect cuticle, and we validate this process in vitro, and offer a protein candidate for fabrication of advanced chitin-based materials.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application. 表达生物活性人类睫状神经营养因子的转基因家蚕在生物医学中的应用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-01 DOI: 10.1111/1744-7917.13442

Weiqun Lan, Wenjing Geng, Xuechun Jiang, Feng Chen, Mingyi Zhou, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li

{"title":"Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application.","authors":"Weiqun Lan, Wenjing Geng, Xuechun Jiang, Feng Chen, Mingyi Zhou, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li","doi":"10.1111/1744-7917.13442","DOIUrl":"https://doi.org/10.1111/1744-7917.13442","url":null,"abstract":"Ciliary neurotrophic factor (CNTF) acts as a potent neuroprotective agent in neuronal survival and regeneration, and can also induce the differentiation of several stem cells into neurons, which highlights the broad application of CNTF in biomedicine. However, large-scale production of bioactive recombinant human CNTF protein remains to be explored. Herein, this study aims to express a bioactive human CNTF protein on a large scale by genetically engineering a silk gland bioreactor of silkworm. Our results showed that CNTF protein was successfully expressed in the middle silk gland (MSG) of silkworm, which can be secreted into the silks with the amount of 3.2 mg/g cocoons. The fabrication of human CNTF-functionalized silk material was able to promote proliferation and migration of neural cells when compared to the natural silk protein. Importantly, this functional silk material could also facilitate neurite outgrowth of mouse retinal ganglion cell (RGC-5) cells. All these data demonstrated a high bioactivity of the recombinant human CNTF protein expressed in the MSG of silkworm. The further fabrication of different silk materials with CNTF bioactivity will give biomedical applications in tissue engineering and neuroregeneration.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142106999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis and functional study of phospholipase A₂ in Galleria mellonella larvae lipid metabolism in response to envenomation by an ectoparasitoid, Iseropus kuwanae. 转录组分析和磷脂酶 A2 在幼虫脂质代谢中的功能研究，以应对外寄生虫 Iseropus kuwanae 的螫伤。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-09-01 DOI: 10.1111/1744-7917.13440

Hanqi Zhu, Xinhao Liang, Jianhao Ding, Jinzheng Wang, Ping Li, Weihong Zhou, Jun Wang, Fu-An Wu, Sheng Sheng

{"title":"Transcriptome analysis and functional study of phospholipase A2 in Galleria mellonella larvae lipid metabolism in response to envenomation by an ectoparasitoid, Iseropus kuwanae.","authors":"Hanqi Zhu, Xinhao Liang, Jianhao Ding, Jinzheng Wang, Ping Li, Weihong Zhou, Jun Wang, Fu-An Wu, Sheng Sheng","doi":"10.1111/1744-7917.13440","DOIUrl":"https://doi.org/10.1111/1744-7917.13440","url":null,"abstract":"There is abundant evidence that parasitoids manipulate their hosts by envenomation to support the development and survival of their progeny before oviposition. However, the specific mechanism underlying host nutritional manipulation remains largely unclear. To gain a more comprehensive insight into the effects induced by the gregarious ectoparasitoid Iseropus kuwanae (Hymenoptera: Ichneumonidae) on the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) larvae, we sequenced the transcriptome of both non-envenomed and envenomed G. mellonella larvae, specifically targeting genes related to lipid metabolism. The present study revealed that 202 differentially expressed genes (DEGs) were identified and 9 DEGs were involved in lipid metabolism. The expression levels of these 9 DEGs relied on envenomation and the duration post-envenomation. Further, envenomation by I. kuwanae induced an increase in triglyceride (TG) level in the hemolymph of G. mellonella larvae. Furthermore, silencing GmPLA2 in G. mellonella larvae 24 h post-envenomation significantly decreased the content of 4 unsaturated fatty acids and TG levels in the hemolymph. The content of linoleic acid and α-linoleic acid were significantly decreased and the content of oleic acid was significantly increased by exogenous supplement of arachidonic acid. Meanwhile, the reduction in host lipid levels impairs the growth and development of wasp offspring. The present study provides valuable knowledge about the molecular mechanism of the nutritional interaction between parasitoids and their hosts and sheds light on the coevolution between parasitoids and host insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142106998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0