Insect Science最新文献_第9页

Exploring marking methods for the predatory hoverfly Sphaerophoria rueppellii (Diptera: Syrphidae). 探索捕食性食蚜蝇 Sphaerophoria rueppellii（双翅目：蚜科）的标记方法。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-16 DOI: 10.1111/1744-7917.70020

Michele Violi, Elena Costi, Elena Monari, Daniele Sommaggio, Lara Maistrello

{"title":"Exploring marking methods for the predatory hoverfly Sphaerophoria rueppellii (Diptera: Syrphidae).","authors":"Michele Violi, Elena Costi, Elena Monari, Daniele Sommaggio, Lara Maistrello","doi":"10.1111/1744-7917.70020","DOIUrl":"https://doi.org/10.1111/1744-7917.70020","url":null,"abstract":"Hoverflies are essential to ecosystems, with adults serving as important pollinators and larvae preying on plant-feeding insects or recycling nutrients. Species like Sphaerophoria rueppellii are used in biocontrol programs to target aphid pests. To enhance these programs, markers can be used in a mark-release-recapture (MRR) method to track hoverfly feeding and oviposition sites. Effective markers must be persistent and not harm the hoverflies' vital functions. This study evaluated three marking methods for S. rueppellii: rubidium (RbCl), fluorescein, and fluorescent dust. Laboratory experiments assessed the effects of these markers on female hoverfly fecundity, mating behavior and marking persistence. Results showed no significant differences in egg-laying or survival time between marked and unmarked females. Rubidium and fluorescein did not affect mating behavior, but dust-treated females mated significantly less than untreated females. In terms of marking persistence, rubidium and fluorescent dusts remained detectable throughout the hoverflies' adult lifespan, while fluorescein markings faded within 24 h. Fluorescent dusts were easy-to-use, durable, and cost-effective, but careful application and further study are needed to avoid potential effects on insect activity and mating ability. Fluorescein showed no adverse effects on insect biology, was economical and quick to apply, but had short persistence, making it unsuitable for long-term field studies. Rubidium was harmless to insects and detectable for long periods, but its detection required financial investment, time, and specialized equipment. This research provides valuable insights into the potential of hoverflies as biocontrol agents and offers new tools for their effective management in agricultural settings.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinsecticide control and enzymatic responses in Spodoptera frugiperda. 果夜蛾生物杀虫剂防治及酶促反应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-16 DOI: 10.1111/1744-7917.70005

Zhuo Li, Chun-Yan Chang, Jia-Rui Deng, Chuan-Lin Zhao, Li-Yuan Yan, Wen Zhang, Ji-Long Xing, Yu-Cheng Sun, Feng Ge

{"title":"Bioinsecticide control and enzymatic responses in Spodoptera frugiperda.","authors":"Zhuo Li, Chun-Yan Chang, Jia-Rui Deng, Chuan-Lin Zhao, Li-Yuan Yan, Wen Zhang, Ji-Long Xing, Yu-Cheng Sun, Feng Ge","doi":"10.1111/1744-7917.70005","DOIUrl":"https://doi.org/10.1111/1744-7917.70005","url":null,"abstract":"Spodoptera frugiperda (Lepidoptera: Noctuidae) is a serious invasive pest, which has attracted concern regarding the effectiveness of environmental bioinsecticide as a substitution for synthetic insecticide in controlling its damage to numerous agricultural crops in recent years. Hence, laboratory and field experiments have investigated insecticidal toxicity in S. frugiperda by 5 insecticides and have determined the activity of acetylcholinesterase (AChE) and detoxifying enzymes, namely mixed function oxidase (MFO), carboxylesterase (CarE), and glutathione S-transferase (GST) on 3rd instar larvae at 25% lethal concentration (LC25) to explore detoxification mechanisms. The results showed that the most effective insecticides were 3.0% emamectin benzoate (0.024 mg/L), 60 g/L spinetoram (0.282 mg/L), and 1.3% matrine (0.380 mg/L) at 48 h depending on LC50 values. The field efficiencies of emamectin benzoate and spinetoram were over 80% at 24 h and 90% at 72 h, indicating that both insecticides had acute and long-lasting toxicity on S. frugiperda; matrine had extended-release toxicity with 90% field efficiency at 72 h. Emamectin benzoate and spinetoram activated the activities of AChE, MFO and GST, and spinetoram decreased in CarE activity; cyantraniliprole induced an increase of 4 detoxifying enzymes; spinosad increased the activities of AChE, MFO, and CarE but not GST. Furthermore, matrine had an inhibiting effect on AChE and acceleration on CarE and GST. Overall results obviously depicted that semi-synthetic insecticide spinetoram and bioinsecticide matrine were recommended to control S. frugiperda with effective and long-lasting toxicity. Moreover, this study will provide basic information for sustainable control of S. frugiperda under field conditions in China.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SGDAcn is a suppressor for silk gland endoreplication and development. sgdcn是丝腺内复制和发育的抑制因子。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-16 DOI: 10.1111/1744-7917.70021

Lin Wu, Li Zhao, Yuting Feng, Jinxin Wu, Xiaoting Hua, Wei Wang, Yuancheng Wang, Zhiqing Li, Qingyou Xia, Ping Lin, Guanwang Shen

{"title":"SGDAcn is a suppressor for silk gland endoreplication and development.","authors":"Lin Wu, Li Zhao, Yuting Feng, Jinxin Wu, Xiaoting Hua, Wei Wang, Yuancheng Wang, Zhiqing Li, Qingyou Xia, Ping Lin, Guanwang Shen","doi":"10.1111/1744-7917.70021","DOIUrl":"https://doi.org/10.1111/1744-7917.70021","url":null,"abstract":"Silkworm silk gland cells undergo multiple rounds of endoreplication, a process in which the genome is duplicated without cell division, leading to cellular polyploidization. This results in the accumulation of genomic DNA, serving as the foundation for rapid silk proteins synthesis. For the first time, we report a previously uncharacterized gene, SGDAcn, in the silkworm silk gland that clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9-mediated SGDAcn knockout in the posterior silk gland increased cell size and enhanced silk production. SGDAcn knockout facilitated the progression of endoreplication by upregulating the expression of various cyclin genes and promoting energy metabolism, leading to a substantial increase in fibroin gene expression and its transcription factor Dimm, as well as the stimulation of ribosome biogenesis for messenger RNA translation and enhancement of eukaryotic translation initiation factors for protein synthesis. Our findings demonstrate that SGDAcn influences endoreplication, cell growth, and nucleolus size through SGDAcn-EGFR/PI3K/AKT and SGDAcn-NF-κB signaling pathways. Overall, SGDAcn acts as a negative regulator of silk gland development, affecting cell size and protein synthesis, thus modulating silk production. These mechanisms might be conserved in cell growth and progression, making an attractive target for genetic editing to improve silk yield in silkworms and potentially in mammalian cell growth regulation.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic consequences of domestication and refreshment on colonies of the South American fruit fly. 南美洲果蝇种群驯化和繁殖的遗传后果。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-13 DOI: 10.1111/1744-7917.70007

Daniel F Paulo, Rosangela A Rodrigues, David Haymer, Adalecio Kovaleski, Carlos Cáceres, Thiago Mastrangelo

{"title":"Genetic consequences of domestication and refreshment on colonies of the South American fruit fly.","authors":"Daniel F Paulo, Rosangela A Rodrigues, David Haymer, Adalecio Kovaleski, Carlos Cáceres, Thiago Mastrangelo","doi":"10.1111/1744-7917.70007","DOIUrl":"https://doi.org/10.1111/1744-7917.70007","url":null,"abstract":"For almost a decade, natural populations of the South American fruit fly have been targeted for control through Sterile Insect Technique projects. To ensure a sustainable supply of competitive sterile flies for this approach, it is essential to understand the effects of domestication when strains of this pest are initially brought into the laboratory to establish colonies as well as the changes occurring after multiple generations of adaptation to conditions used for mass rearing. Using one colony established from a wild population of the Brazil-1 morphotype (WIL) and two from laboratory colonies in Brazil known as the Piracicaba (PL) and Vacaria (VL) strains, this study evaluated genetic diversity in samples from 10 generations after domestication and maintenance under semimass rearing conditions. Another aim of this study was to analyze changes in the genetic makeup of the colonies of the two laboratory strains after refreshment. Eight microsatellite markers were used for the genetic analyses. Results indicated a moderate but significant amount of genetic differentiation between the WIL population and the two laboratory strains. Results also showed that levels of genetic diversity in both the VL and PL strains were maintained at similar levels over a period of more than two years of rearing. Additionally, results suggest that successful creation of admixture via refreshment is more likely to be beneficial in relatively short-term domesticated colonies, and that performing refreshment approximately every six to eight generations could be beneficial to maintain the genetic diversity of A. fraterculus colonies under laboratory mass rearing conditions.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diaphorina citri E3 ubiquitin ligase RNF115 inhibits CLas bacterial proliferation by targeting to the host histone H1. 柑橘木虱E3泛素连接酶RNF115通过靶向宿主组蛋白H1抑制CLas细菌增殖。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-13 DOI: 10.1111/1744-7917.70022

Xiao-Jin Zou, Yi-Hong Zhang, Can Zhang, Xiao-Fang Yuan, Meng-Jun Yun, Lian-Jie Xie, Xiao-Qiang Liu, Wen-Feng Kang, Wei Chen, Ying-Xue Liu, Ai-Yun Wang, Zhan-Jun Lu, Hai-Zhong Yu

{"title":"Diaphorina citri E3 ubiquitin ligase RNF115 inhibits CLas bacterial proliferation by targeting to the host histone H1.","authors":"Xiao-Jin Zou, Yi-Hong Zhang, Can Zhang, Xiao-Fang Yuan, Meng-Jun Yun, Lian-Jie Xie, Xiao-Qiang Liu, Wen-Feng Kang, Wei Chen, Ying-Xue Liu, Ai-Yun Wang, Zhan-Jun Lu, Hai-Zhong Yu","doi":"10.1111/1744-7917.70022","DOIUrl":"https://doi.org/10.1111/1744-7917.70022","url":null,"abstract":"The Asian citrus psyllid (ACP), Diaphorina citri, serves as the primary vector for Candidatus Liberibacter asiaticus (CLas), the pathogen responsible for citrus Huanglongbing (HLB). D. citri modulates the expression of its key proteins in response to CLas infection. Previous research has revealed that CLas infection significantly alters the expression levels of E3 ubiquitin ligases in D. citri; however, the specific functions of these E3 ligases remain largely uncharacterized. In this study, a total of 11 E3 ubiquitin ligases were identified from the proteomics database of D. citri, among which E3 ubiquitin ligase RNF115 was significantly upregulated following CLas infection. RING finger protein 115 (RNF115) consists of 156 amino acids and contains a RING finger domain at its N-terminus. Silencing RNF115 via RNA interference (RNAi) and injecting the inhibitor disulfiram, which targets RNF115, significantly increased CLas bacterial content in D. citri. In contrast, injection of recombinant RNF115 protein markedly inhibited CLas bacterial proliferation. Furthermore, interaction between RNF115 and D. citri histone H1 was confirmed using yeast 2-hybrid assay, pull-down experiments and molecular docking analysis. Knockdown of histone H1 via RNAi significantly reduced CLas bacterial content, whereas injection of recombinant histone H1 protein led to an increase in CLas content within D. citri. These findings suggest that CLas infection may induce an upregulation of RNF115 expression in D. citri, leading to subsequent interactions with histone H1 that facilitate the ubiquitination of histone H1, ultimately resulting in reduced expression levels and inhibiting CLas proliferation within D. citri.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Doublesex regulates male sexual development in the cricket Gryllus bimaculatus. 双性调节蟋蟀雄性的性发育。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-11 DOI: 10.1111/1744-7917.70019

Li-Dong Shi, Jia-Xin Duan, Gang-Qi Fang, Yun Bai, Mi Di, Xiao Zhang, Dong-Liang Li, Zhu-Qing He, Kai Li

{"title":"Doublesex regulates male sexual development in the cricket Gryllus bimaculatus.","authors":"Li-Dong Shi, Jia-Xin Duan, Gang-Qi Fang, Yun Bai, Mi Di, Xiao Zhang, Dong-Liang Li, Zhu-Qing He, Kai Li","doi":"10.1111/1744-7917.70019","DOIUrl":"https://doi.org/10.1111/1744-7917.70019","url":null,"abstract":"Insects have evolved a diversity of regulatory mechanisms to determine their sex. Understanding the molecular regulation mechanisms of insect sex determination is of great significance in revealing the general law of insect sex determination and providing potential routes for the genetic manipulation of pest species. Although the sex determination cascade and doublesex (dsx) gene functions have been well described in some holometabolous insects, little is known about this cascade in hemimetabolous insects. In this study, we identified the dsx homolog in Gryllus bimaculatus, which belongs to the Orthoptera order and is an important model for developmental and evolutionary biology. We found that Gbdsx has two alternative splicing isoforms (male-specific GbdsxM and non-sex-specific GbdsxC). Using RNAi-mediated knock-down of GbdsxM in 6th-instar nymphs resulted in adult male forewings showing feminized vein development and abnormal external genitalia. CRISPR/Cas9 knockout of Gbdsx in embryos resulted in adult males becoming pseudofemales, with feminized forewings and abnormal external and internal genitalia. Additionally, the pseudofemales created by Gbdsx knockout demonstrated normal courtship trends and aggressive behavior but no actual mating behavior. However, the knockout and knock-down of Gbdsx in female crickets does not affect their sexual traits or fertility. Our results suggest that GbdsxM plays a critical role in the development of male cricket sexual traits, courtship and mating behavior, which furthers our understanding of sex determination in hemimetabolous insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

m-Aminophenylacetylene shortened the eggsac-carrying stages in the wolf spider through enhancing the synthesis of prostaglandins. 间氨基苯乙炔通过促进前列腺素的合成而缩短了狼蛛的携卵期。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-11 DOI: 10.1111/1744-7917.70017

Yunru Chen, Tianhong Xu, Yuan Wang, Jingting Wang, Chenkai Zhao, Na Yu, Zewen Liu

{"title":"m-Aminophenylacetylene shortened the eggsac-carrying stages in the wolf spider through enhancing the synthesis of prostaglandins.","authors":"Yunru Chen, Tianhong Xu, Yuan Wang, Jingting Wang, Chenkai Zhao, Na Yu, Zewen Liu","doi":"10.1111/1744-7917.70017","DOIUrl":"https://doi.org/10.1111/1744-7917.70017","url":null,"abstract":"Spiders are important predatory enemies that control a range of insect pests in the rice ecosystem. m-Aminophenylacetylene (m-A) has been demonstrated to induce maternal care behaviors in Pardosa pseudoannulata, protecting young spiders and promoting population growth. Here the roles of m-A on other reproduction stages were evaluated by injecting m-A into virgin and postreproductive female spiders. When injected into virgin females, m-A can prolong the first pulli-carrying stage of the females after mating and breeding offsprings. In postreproductive females, m-A injection shortened the eggsac-carrying stages, especially in the second and third stages. Transcriptomic analysis showed that differentially expressed genes between the control female and m-A-injected female were enriched in the arachidonic acid (AA) metabolism pathway. Real-time quantitative PCR (qPCR) confirmed the upregulation of cyclooxygenase-II (COX-II), prostaglandin E synthase 2 (PGES2), and prostaglandin E synthase 3 (PGES3) genes involved in PGE2 (prostaglandin E2) synthesis. In a reverse validation experiment, the sequential injection of m-A followed by a COX inhibitor aspirin restored the shortened eggsac-carrying stage to normal level. The findings indicated that m-A enhanced PGs synthesis, leading to a reduced eggsac-carrying stage. The eggsac-carrying stage can be fatal when female spiders encounter their enemies, so the shortening of the stage by m-A would reduce the potential threat. These findings are significant for developing environmentally friendly control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IIS/TOR network plays an essential role in ovarian development in the pupal stage of the fall armyworm, Spodoptera frugiperda. 在秋粘虫（Spodoptera frugiperda）蛹期，IIS/TOR网络在卵巢发育中起重要作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-10 DOI: 10.1111/1744-7917.70015

Yufang Yan, Zhiyong Yin, Qin Lu, Tongxian Liu, Samiullah Khan, Xinyi Ma, Haiyin Li, Jianjun Guo

{"title":"IIS/TOR network plays an essential role in ovarian development in the pupal stage of the fall armyworm, Spodoptera frugiperda.","authors":"Yufang Yan, Zhiyong Yin, Qin Lu, Tongxian Liu, Samiullah Khan, Xinyi Ma, Haiyin Li, Jianjun Guo","doi":"10.1111/1744-7917.70015","DOIUrl":"https://doi.org/10.1111/1744-7917.70015","url":null,"abstract":"The ovary plays a crucial role in the female reproductive system of insects and is vital for the perpetuation of insect populations. The insulin/insulin-like growth factor signaling (IIS) and target of rapamycin (TOR) signaling network play a key role in controlling ovarian development and maturation during the adult stage. However, the pupal stage is a critical developmental phase in the reproductive system of holometabolous insects, and the regulatory role of the IIS/TOR network during this stage remains to be elucidated. In this study, we observed that during the pupal stage, the enlargement of ovarian development was accompanied by a corresponding increase in messenger RNA expression levels of genes within the IIS/TOR signaling pathway and insulin levels in the hemolymph. Decapitation experiments, serving as a pivotal approach to assess the regulatory role of the IIS signaling pathway in ovarian development, demonstrated that decapitation markedly inhibited ovarian development at this critical stage. Exogenous insulin administration significantly increased the phosphorylation levels of FOXO, S6K, and 4E-BP, thereby enhancing ovarian development and resulting in significant elongation of the ovarioles. In contrast, the injection of IIS/TOR pathway inhibitors, LY294002 and Rapamycin, reduced the phosphorylation of these proteins, inhibiting ovarian development during the pupal stage and leading to a significant shortening of the ovarioles, negatively impacting the reproductive performance of adult females. The current findings indicate that the IIS/TOR signaling network significantly regulates ovarian development during the pupal stage of Spodoptera frugiperda, providing new molecular insights for the development of pest management strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and function analysis of two new gustatory receptors related to silkworm monophagy. 家蚕单食相关的两种新的味觉受体鉴定及功能分析。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-06 DOI: 10.1111/1744-7917.70006

Jian-Zhao Jiang, Qin Xiao, Zhuo-Lin Gao, Jun Gu, Li-Hua Huang

{"title":"Identification and function analysis of two new gustatory receptors related to silkworm monophagy.","authors":"Jian-Zhao Jiang, Qin Xiao, Zhuo-Lin Gao, Jun Gu, Li-Hua Huang","doi":"10.1111/1744-7917.70006","DOIUrl":"https://doi.org/10.1111/1744-7917.70006","url":null,"abstract":"Silkworm is a typical monophagous insect that can only feed on fresh mulberry leaves. The mechanism for this monophagous nature is not fully understood. One bitter gustatory receptor (GR) GR66 located on the maxilla of the mouthpart has been reported to be an important factor influencing the feeding preference of silkworm. However, the preference of GR66 mutants for a nonhost plant was very low, suggesting that other factors related to silkworm monophagy need to be further explored. In this study, 10 bitter GRs were screened out based on their specific high expression in the maxilla of silkworm, and the 4 most expressed GRs (GR15, GR43, GR69, and GR66) were knocked out by clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9. Feeding experiments showed that except for GR69-/-, the host range of GR43-/-, GR15-/-, and GR66-/- expanded remarkably, and both GR43-/- and GR66-/- revealed the highest preference for the nonhost plants. Moreover, the strict preference for mulberry leaves was almost eliminated in the double mutant silkworms of GR43-/- and GR66-/-. These results imply that it is likely that a variety of different bitter GRs are involved in determining the monophagy of silkworm larvae. In addition, GR15 mutation significantly inhibited ovarian development, resulting in a significant decrease in oviposition. These findings enhance our understanding of the monophagous nature of silkworms and provide a possibility for the molecular breeding of polyphagous silkworms in the future.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regional heterothermy in Megasoma gyas is not related to active heat dissipation by the horns. 巨瘤的区域异热与角的主动散热无关。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-03-06 DOI: 10.1111/1744-7917.70018

Danilo Giacometti, Luiz Henrique Lima Silva, Guilherme Gomes, José Eduardo de Carvalho, Alexandre V Palaoro

引用次数: 0