Insect Science最新文献_第9页

Spiroplasma infection in colonized Glossina fuscipes fuscipes: impact on mass rearing and the sterile insect technique. 定植fuscipes fuscipes的螺旋体感染：对大规模饲养和昆虫不育技术的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-26 DOI: 10.1111/1744-7917.70078

Kiswend-Sida M Dera, Daouda Tande Barro, Bénéwendé Aristide Kaboré, Fabian Gstöttenmayer, Mouhamadou M Dieng, Soumaïla Pagabeleguem, Brian L Weiss, Giulia Fiorenza, Riccardo Piccinno, Anna R Malacrida, Serap Aksoy, Chantel J de Beer, Robert L Mach, Marc J B Vreysen, Adly M M Abd-Alla

{"title":"Spiroplasma infection in colonized Glossina fuscipes fuscipes: impact on mass rearing and the sterile insect technique.","authors":"Kiswend-Sida M Dera, Daouda Tande Barro, Bénéwendé Aristide Kaboré, Fabian Gstöttenmayer, Mouhamadou M Dieng, Soumaïla Pagabeleguem, Brian L Weiss, Giulia Fiorenza, Riccardo Piccinno, Anna R Malacrida, Serap Aksoy, Chantel J de Beer, Robert L Mach, Marc J B Vreysen, Adly M M Abd-Alla","doi":"10.1111/1744-7917.70078","DOIUrl":"https://doi.org/10.1111/1744-7917.70078","url":null,"abstract":"Tsetse flies (Glossina spp.) can vector the parasites (Trypanosoma spp.) that cause the socioeconomically devastating neglected tropical diseases human and animal African trypanosomoses. In addition to this parasite, tsetse can harbor four genera of endosymbiotic bacteria, including Wigglesworthia, Sodalis, Wolbachia, and Spiroplasma, which are functionally crucial for the fly's physiological homeostasis and/or are potentially useful for the development of disease control strategies. Recent discoveries indicate that Spiroplasma infection negatively impacts tsetse fecundity. Conversely, housing the bacterium can benefit its fly host by making it unusually refractory to infection with parasitic African trypanosomes. In this study, we assessed the physiological impact of Spiroplasma infection on a laboratory colony of Glossina fuscipes fuscipes (Gff). For this purpose, two distinct Gff colonies were established: a Spi- colony that harbors a low Spiroplasma infection prevalence and a Spi+ colony that harbors a high Spiroplasma infection prevalence. Fitness parameters for both colonies revealed no significant differences in the length of larval development, adult eclosion rate, and flight propensity. However, flies from the Spi+ colony presented with lower fecundity and higher overall mortality than did individuals from the Spi- colony. Furthermore, males from the Spi- colony exhibited a competitive mating advantage over their Spi+ counterparts in a field cage setting. These findings have potential implications for the improvement of mass-rearing of Gff for sterile insect technique (SIT) applications.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive transcriptomics comparison reveals switch of gene networks in the brain during silkworm domestication. 综合转录组学比较揭示了家蚕驯化过程中脑内基因网络的开关。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-26 DOI: 10.1111/1744-7917.70077

Xiang-Min Wei, Yong Cui, Yong-Jian Lin, Jia-Hao Xiang, Ke-Sen Zhu, Hui Xiang, Man Wang

{"title":"Comprehensive transcriptomics comparison reveals switch of gene networks in the brain during silkworm domestication.","authors":"Xiang-Min Wei, Yong Cui, Yong-Jian Lin, Jia-Hao Xiang, Ke-Sen Zhu, Hui Xiang, Man Wang","doi":"10.1111/1744-7917.70077","DOIUrl":"https://doi.org/10.1111/1744-7917.70077","url":null,"abstract":"As in other domestic animals, the silkworm Bombyx mori has evolved adaptive behavioral changes compared with its wild ancestor, Bombyx mandarina. Domestication may have significantly impacted the brain of the silkworm, but it remains unclear whether and how the brain has evolved in response to domestication. Here we systematically quantified and compared morphological differences in the brains of domestic and wild silkworms at 7 representative stages during metamorphosis, and revealed that the B. mori brain had a reduced brain area and a reduced degree of fusion compared with the B. mandarina brain. The 2 silkworm species exhibited divergent brain developmental transcriptomic landscapes, implying the reshaping of brain functions during domestication. Genes enriched in nervous system development were generally repressed in the domesticated brain, with a slight upregulation during the wandering stage. An upregulation of Toll and Imd and a disturbance of apoptotic homeostasis were identified in the domestic silkworm brain. Mapping a comprehensive interaction network of these pathways and nervous system-related processes identified one key gene, Stx1b, and several candidate domestication genes that may have coordinated the developmental changes in the brain of the silkworm during domestication.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding biological control function and trophic interaction dynamics of an artificially released predatory bug by DNA metabarcoding. 利用DNA元条形码技术了解人工释放捕食性昆虫的生物防治功能和营养相互作用动力学。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-25 DOI: 10.1111/1744-7917.70074

Weidong Huang, Suyi Chen, Zihua Zhao, Chunyan Yang, Anxian Shi, Hu Li, Yuange Duan, Fan Song, Hailin Yang, Wanzhi Cai

{"title":"Understanding biological control function and trophic interaction dynamics of an artificially released predatory bug by DNA metabarcoding.","authors":"Weidong Huang, Suyi Chen, Zihua Zhao, Chunyan Yang, Anxian Shi, Hu Li, Yuange Duan, Fan Song, Hailin Yang, Wanzhi Cai","doi":"10.1111/1744-7917.70074","DOIUrl":"https://doi.org/10.1111/1744-7917.70074","url":null,"abstract":"Understanding the dietary habits of generalist invertebrate predators in agroecosystems helps improve ecosystem programs, particularly for integrated pest management. Augmentation biological control, one of three widely accepted biological pest control methods, involves releasing a large number of natural enemies to quickly reduce damaging pest populations. However, the effectiveness of these natural enemies against target pests and their effects on nontarget pests in agricultural landscapes remain unknown. This study aimed to investigate the dietary composition of Sycanus bifidus, a generalist predator of the assassin bug that is artificially released into citrus orchards, and uncover its trophic structure across various pest species using metabarcoding-based molecular gut content analysis. We detected 30 different prey taxa from 8 orders and 19 families in 108 samples of S. bifidus recaptured over 22 d. Lepidoptera (9) made up 30% of their diet, followed by Coleoptera (5), Hemiptera (5), and Hymenoptera (5). Euproctis varians, a major lepidopteran citrus pest, was frequently found in the gut of S. bifidus with a high incidence and relative read abundance, suggesting its potential as an effective biological control agent for E. varians in citrus crops. Additionally, our findings showed that the choice of primer pair critically influenced the efficiency of diet detection, with the Fwh primer set being ideal for targeting highly degraded DNA in insect guts. This study demonstrated that metabarcoding is a valuable tool for studying predator diets, reconstructing trophic interactions in agricultural landscapes, optimizing pest management strategies, and identifying effective biological control agents.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duplicated paralog of insulin receptor functions distinctively in locust reproduction for high fecundity. 胰岛素受体的重复平行功能在蝗虫繁殖中具有显著的高繁殖力。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-25 DOI: 10.1111/1744-7917.70076

Libin Yang, Yan Liu, Zhongxia Wu, Shutang Zhou

{"title":"Duplicated paralog of insulin receptor functions distinctively in locust reproduction for high fecundity.","authors":"Libin Yang, Yan Liu, Zhongxia Wu, Shutang Zhou","doi":"10.1111/1744-7917.70076","DOIUrl":"https://doi.org/10.1111/1744-7917.70076","url":null,"abstract":"Insulin receptor (InR) mediates the highly conserved insulin/insulin-like growth factor signaling pathway that regulates a broad range of life-history traits. Most insects encode 2 InR paralogs, but their functional divergence and redundancy remain to be explored. Here, we pursued the functionality of 2 InRs, LmInR1, and LmInR2, in the migratory locust Locusta migratoria. LmInR1 is clustered into the clade of ancestral InR, and LmInR2 belongs to the clade of InR gene duplication. While LmInR1 expression was at the highest levels in nymphal stage and adult ovary, LmInR2 was predominantly expressed in adult fat body. Loss of LmInR1 function led to defective nymph-adult transition and reproduction. LmInR2 depletion had little effect on metamorphosis, but caused severe defects in female reproduction, including remarkable reduction of Vitellogenin (Vg) expression and arrested egg development. Interestingly, LmInR1 expression responded to lower levels of glucose, whereas LmInR2 was expressed in response to increasing concentrations of glucose. Moreover, the expression of LmInR2 but not LmInR1 was responsive to juvenile hormone (JH) and its receptor. The results suggest that LmInR2 has evolved neofunctionalization for massive Vg synthesis required for synchronous maturation of dozens of oocytes in a high-fecundity insect. This study reveals a novel aspect of differential functions of InR paralogs and provides new insights into understanding of the insulin signaling cascade in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signaling pathways in Drosophila testis niche: Local signals that regulate stem cell fate. 果蝇睾丸生态位的信号通路：调节干细胞命运的局部信号。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-20 DOI: 10.1111/1744-7917.70072

Ting Zhao, Xiao-Qiang Yu

引用次数: 0

BmADARa cooperatively inhibits BmNPV proliferation through the interaction of its dsRBD2 with BmDcr-2-DEXHc in silkworm, Bombyx mori. BmADARa通过其dsRBD2与BmDcr-2-DEXHc在家蚕中协同抑制BmNPV增殖。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-20 DOI: 10.1111/1744-7917.70073

Song Jiang, Chong-Jun Ye, Yu-Chen Wu, Ruo-Yun Shi, Yu-Long Yu, Syeda Saneela, Dan Liang, Yan-Jiao Huang, Xia-Ming Shi, Yan Meng

{"title":"BmADARa cooperatively inhibits BmNPV proliferation through the interaction of its dsRBD2 with BmDcr-2-DEXHc in silkworm, Bombyx mori.","authors":"Song Jiang, Chong-Jun Ye, Yu-Chen Wu, Ruo-Yun Shi, Yu-Long Yu, Syeda Saneela, Dan Liang, Yan-Jiao Huang, Xia-Ming Shi, Yan Meng","doi":"10.1111/1744-7917.70073","DOIUrl":"https://doi.org/10.1111/1744-7917.70073","url":null,"abstract":"Adenosine deaminases that act on RNA (ADARs) are RNA editing enzymes capable of converting adenosine into inosine at specific sites within double-stranded RNA (dsRNA), widely distributed across various animal species. Dicer (Dcr), a member of the RNase III family and a crucial component of the RNA-induced silencing complex (RISC), allows ADAR to participate in innate immunity through Dcr-2 in Drosophila. Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the viruses that can cause substantial economic losses to the sericulture industry upon infecting silkworm. Knocking down the expression of BmDcr-2 in silkworm enhances the proliferation of BmNPV. Our previous research revealed the existence of a predominantly expressed subtype, ADARa, in silkworm (BmADARa), which shares homology with Drosophila ADAR. It remains unclear whether BmADARa can also participate in innate immunity through BmDcr-2. Initially, through bacterial challenge experiments, we found that BmADARa exhibited the highest responsiveness to BmNPV stimulation. Further studies demonstrated that BmADARa, in conjunction with BmDcr-2-DEXHc (DEAD-box helicase domain), collectively inhibits the proliferation of BmNPV. BmADARa interacts with the DEXHc domain of BmDcr-2 through its dsRNA binding domain 2 (dsRBD2), thereby enhancing its ability to inhibit BmNPV proliferation. These results lay a foundation for the study of the function and molecular mechanism of BmADARa in innate immunity, and provide a new experimental ideas for antiviral research in B. mori.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening optimum reference genes for quantitative real-time polymerase chain reaction analysis in black soldier fly Hermetia illucens under different conditions. 筛选不同条件下黑兵蝇实时定量聚合酶链反应的最佳内参基因。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-20 DOI: 10.1111/1744-7917.70067

Qiuqin Ma, Zhending Gan, Xiaomei Ye, Ruju Zhang, Ting Han, Jing Du, Fei Zhu

{"title":"Screening optimum reference genes for quantitative real-time polymerase chain reaction analysis in black soldier fly Hermetia illucens under different conditions.","authors":"Qiuqin Ma, Zhending Gan, Xiaomei Ye, Ruju Zhang, Ting Han, Jing Du, Fei Zhu","doi":"10.1111/1744-7917.70067","DOIUrl":"https://doi.org/10.1111/1744-7917.70067","url":null,"abstract":"With the completion of genome sequencing of the black soldier fly (BSF), an increasing number of researchers are focusing on elucidating its robust digestive and absorptive capabilities through genetic and molecular biology methods to enhance its growth performance. However, most genetic and molecular biology studies require gene expression analysis, and the stability of reference genes may change under different experimental conditions. To identify optimal reference genes, we evaluated 11 candidate reference genes (GAPDH, β-actin, GST, Tubulin α-1A, Tubulin α-4A, Tubulin β, RP49, RPL13, EF1-α, 18S RNA, and 28S RNA) across various developmental stages, tissues, sexes, and 3 distinct genotypes of Hermetia illucens using RefFinder software, which integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and ΔCt. The results recommend that β-Actin, 18S RNA, and RPL13 are suitable for analyzing different developmental stages; RPL13, GST, and RP49 are optimal for different tissues; EF1, RPL13, and GST work best for different sexes; and RPL13, GAPDH, and 18S RNA are ideal for different genotypes. Notably, this study is the first to evaluate the stability of reference genes across 3 BSF genotypes originating from different regions. The findings offer valuable references and fresh insights for molecular biology research on H. illucens.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Eocene and modern entomofauna differ-a Cretaceous-like larva in Rovno amber". 对“始新世和现代昆虫动物的差异——罗夫诺琥珀中白垩纪样幼虫”的修正。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-14 DOI: 10.1111/1744-7917.70083

引用次数: 0

SV2B is a crucial factor for early larval development in the silkworm, Bombyx mori. SV2B是家蚕早期幼虫发育的关键因子。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-14 DOI: 10.1111/1744-7917.70070

Yuting Zhang, Zhiping Xing, Hui Dong, Tao Lu, Yuping Deng, Zhipeng Li, Bo Hu, Anjiang Tan

{"title":"SV2B is a crucial factor for early larval development in the silkworm, Bombyx mori.","authors":"Yuting Zhang, Zhiping Xing, Hui Dong, Tao Lu, Yuping Deng, Zhipeng Li, Bo Hu, Anjiang Tan","doi":"10.1111/1744-7917.70070","DOIUrl":"https://doi.org/10.1111/1744-7917.70070","url":null,"abstract":"Synaptic vesicle glycoprotein 2B (SV2B) gene plays a crucial role in neuromodulation and neurotransmission and is a key regulator of synaptotagmin trafficking. However, physiological functions of this gene in insects remain poorly understood. In this study, we investigated the function of the BmSV2B gene in growth and development of silkworms. Tissue expression profiling revealed that BmSV2B is highly expressed in head and midgut. A phylogenetic tree and sequence alignment demonstrated that this gene is highly conserved among lepidopteran insects. Knockout of BmSV2B using the clustered regularly interspaced small palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9) system resulted in smaller body size compared to the wild type (WT) strain. In the BmSV2B mutants, the levels of triacylglycerol were dramatically lower than that in WT. Furthermore, we found that deletion of BmSV2B extended the developmental time of larvae and led to early larval death. High-throughput RNA sequencing and quantitative real-time polymerase chain reaction analysis showed that the expression levels of juvenile hormone-degrading genes, digestive genes, 20-hydroxyecdysone -response genes and forkhead box O (FOXO) were significantly affected by the absence of BmSV2B. Taken together, BmSV2B is essential for early larval development in silkworms and could serve as a potential target for insecticides, offering a more effective approach to pest control management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycotoxins-contaminated wheat matrices bioconversion by Tenebrio molitor larvae (Coleoptera: Tenebrionidae). 霉毒素污染的小麦基质在黄粉虫幼虫中的生物转化。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-05-13 DOI: 10.1111/1744-7917.70066

Valentina Candian, Valentina Scarpino, Alessandro Bona, Rosemarie Tedeschi, Massimo Blandino

{"title":"Mycotoxins-contaminated wheat matrices bioconversion by Tenebrio molitor larvae (Coleoptera: Tenebrionidae).","authors":"Valentina Candian, Valentina Scarpino, Alessandro Bona, Rosemarie Tedeschi, Massimo Blandino","doi":"10.1111/1744-7917.70066","DOIUrl":"https://doi.org/10.1111/1744-7917.70066","url":null,"abstract":"Tenebrio molitor is proposed for the valorization of mycotoxins-contaminated substrates no longer usable for other purposes. Larvae were reared on three different wheat matrices (wholegrain flour, shorts and bran) contaminated with different levels of mycotoxins. Nine diets (3 matrices × 3 contamination levels) were assessed. Larval development time, average daily gain (ADG), substrate consumption, survival rate, and the capacity of T. molitor to accumulate and excrete different mycotoxins, through exuviae and frass, were evaluated. Larval development time, ADG, survival rate and substrate consumption were not negatively affected by the mycotoxins, but only by the rearing matrix, depending on the nutritive value. The ability of larvae to excrete DON and its derivatives through exuviae and frass was observed. Within each matrix, DON contamination in larvae increased significantly moving from poorly to highly contaminated ones. Overall, the ratio between the modified form (deoxynivalenol-3-glucoside) and the native one (DON), increased from 7%, in the raw materials, to 101% in the larvae, highlighting their ability to modify this mycotoxin and accumulate it in their body. Larvae accumulated also nivalenol, zearalenone, and enniatins showing a higher concentration in larvae reared on substrates with high level of contamination, while moniliformin was never found in larvae. Overall, the levels of mycotoxins recorded in larvae was always below the current legal limits for livestock feed, thanks to their ability to excrete them through exuviae and frass. These interesting data open new scenarios on the valorization of mycotoxin-contaminated matrices, not suitable for other livestock farming, by means of insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0