Insect Science最新文献_第10页

Rainfall during the night can trigger non-migratory take-off behavior of the white-backed planthopper, Sogatella furcifera. 夜间降雨会引发白背飞虱（Sogatella furcifera）的非迁徙性起飞行为。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-27 DOI: 10.1111/1744-7917.13347

Haibo Yang, Yunlong Feng, Pinhong Zhu, Dingxu Li, Gao Hu

{"title":"Rainfall during the night can trigger non-migratory take-off behavior of the white-backed planthopper, Sogatella furcifera.","authors":"Haibo Yang, Yunlong Feng, Pinhong Zhu, Dingxu Li, Gao Hu","doi":"10.1111/1744-7917.13347","DOIUrl":"10.1111/1744-7917.13347","url":null,"abstract":"Take-off behavior is crucial to the overall success of insect migration. Although most high-altitude migratory flights commence with mass take-offs around dusk and dawn, little is known about nighttime take-off behavior. The take-off behavior of migratory Sogatella furcifera was investigated in field cages from 2017 to 2019. The species showed a bimodal take-off pattern at dusk and dawn on rainless nights, with mass flight at dusk more intense than dawn flight. However, a higher frequency of take-offs during the nighttime was observed on rainy nights, resulting in the absence of dawn take-offs. Most migratory take-off individuals at dusk and dawn landed on the cage top or the walls above 150 cm, while non-migratory individuals that took off during the nighttime due to rainfall mainly landed on the cage walls below 150 cm. Furthermore, it has been observed that migratory take-off individuals possess stronger sustained flight capabilities and exhibit more immature ovaries compared with non-migratory take-offs. These findings advance our understanding of the take-off behavior of S. furcifera and thus provide a basis for the accurate prediction and management of the migratory dynamics of this pest.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1908-1917"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into paternity skew in a polyandrous social wasp. 多雄性社会黄蜂亲子关系偏斜的新见解。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-28 DOI: 10.1111/1744-7917.13343

Sarah E Orr, Nicole A Hedrick, Kayla A Murray, Abhinav K Pasupuleti, Michael A D Goodisman

{"title":"Novel insights into paternity skew in a polyandrous social wasp.","authors":"Sarah E Orr, Nicole A Hedrick, Kayla A Murray, Abhinav K Pasupuleti, Michael A D Goodisman","doi":"10.1111/1744-7917.13343","DOIUrl":"10.1111/1744-7917.13343","url":null,"abstract":"Females of many species are polyandrous. However, polyandry can give rise to conflict among individuals within families. We examined the level of polyandry and paternity skew in the common eastern yellowjacket wasp, Vespula maculifrons, in order to gain a greater understanding of conflict in social insects. We collected 10 colonies of V. maculifrons and genotyped workers and prereproductive queens at highly variable microsatellite markers to assign each to a patriline. Genotypic data revealed evidence of significant paternity skew among patrilines. In addition, we found that patrilines contributed differentially to caste production (worker vs. queen), suggesting an important role for reproductive conflict not previously discovered. We also investigated if patterns of paternity skew and mate number varied over time. However, we found no evidence of changes in levels of polyandry when compared to historical data dating back almost 40 years. Finally, we measured a suite of morphological traits in individuals from the most common and least common patrilines in each colony to test if males that showed highly skewed reproductive success also produced offspring that differed in phenotype. Our data revealed weak correlation between paternity skew and morphological phenotype of offspring sired by different males, suggesting no evidence of evolutionary tradeoffs at the level investigated. Overall, this study is the first to report significant paternity and caste-associated skew in V. maculifrons, and to investigate the phenotypic consequences of skew in a social wasp. Our results suggest that polyandry can have important consequences on the genetic and social structure of insect societies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1876-1888"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation in the triosephosphate isomerase gene of the fall armyworm and its distribution across China. 秋梢虫三糖磷酸异构酶基因的遗传变异及其在中国的分布。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-27 DOI: 10.1111/1744-7917.13348

Xin-Yue Liang, Lei Zhang, Hong-Ran Li, Xiao-Ping Niu, Yu-Tao Xiao

{"title":"Genetic variation in the triosephosphate isomerase gene of the fall armyworm and its distribution across China.","authors":"Xin-Yue Liang, Lei Zhang, Hong-Ran Li, Xiao-Ping Niu, Yu-Tao Xiao","doi":"10.1111/1744-7917.13348","DOIUrl":"10.1111/1744-7917.13348","url":null,"abstract":"The fall armyworm (FAW), Spodoptera frugiperda, has colonized and caused consistent damage in the Eastern hemisphere. The identification of various FAW strains is essential for developing precise prevention and control measures. The triosephosphate isomerase (Tpi) gene is recognized as an effective marker closely linked to FAW subpopulations. However, most current studies primarily focus on the comparison of variations in specific gene sites of this gene. In this study, we conducted full-length sequencing of the Tpi genes from 5 representative FAW groups. Our findings revealed that the Tpi genes varied in length from 1220 to 1420 bp, with the primary variation occurring within 4 introns. Notably, the exon lengths remained consistent, at 747 bp, with 37 observed base variations; however, no amino acid variations were detected. Through sequence alignment, we identified 8 stable variation sites that can be used to distinguish FAW strains in the Eastern hemisphere. Additionally, we performed strain identification on 1569 FAW samples collected from 19 provinces in China between 2020 and 2021. The extensive analysis indicated the absence of the rice strain in the samples. Instead, we only detected the presence of the corn strain and the Zambia strain, with the Zambia strain being distributed in a very low proportion (3.44%). Furthermore, the corn strain could be further categorized into 2 subgroups. This comprehensive study provides a valuable reference for enhancing our understanding of FAW population differentiation and for improving monitoring and early warning efforts.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1984-1997"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae). 群居繁殖的甲虫 Necrodes littoralis（Staphylinidae: Silphinae）在腐肉上形成的幼虫群中的竞争、合作和亲代效应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-13 DOI: 10.1111/1744-7917.13353

Natalia Lis, Anna Mądra-Bielewicz, Jędrzej Wydra, Szymon Matuszewski

{"title":"Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae).","authors":"Natalia Lis, Anna Mądra-Bielewicz, Jędrzej Wydra, Szymon Matuszewski","doi":"10.1111/1744-7917.13353","DOIUrl":"10.1111/1744-7917.13353","url":null,"abstract":"Aggregations of juveniles are dominant forms of social life in some insect groups. Larval societies are shaped by competitive and cooperative interactions of the larvae, in parallel with parental effects. Colonies of necrophagous larvae are excellent systems to study these relationships. Necrodes littoralis (Staphylinidae: Silphinae), a carrion beetle that colonizes cadavers of large vertebrates, forms massive juvenile aggregations. By spreading over carrion anal and oral exudates, the beetles form the feeding matrix, in which the heat is produced and by which adults presumably affect the fitness of the larvae. We predict that exploitative competition shapes the behavior of N. littoralis larvae in their aggregations. However, cooperative interactions may also operate in these systems due mainly to the benefits of collective exodigestion. Moreover, indirect parental effects (i.e., formation of the feeding matrix) probably modulate larval interactions within the aggregations. By manipulating parental effects (present/absent) and larval density (0.02-1.9 larvae/g of meat), we found a strong negative group-size effect on fitness components of N. littoralis, in colonies with parental effects over almost the entire density range, and in colonies without parental effects for densities larger than 0.5 larva/g. This was accompanied by positive group-size effects in terms of development time (it shortened with larval density) and thermogenesis (it increased with larval density). A pronounced positive group-size effect on juvenile fitness was found only in colonies without parental effects and only in the low-density range. These results support the hypothesis that larval societies of N. littoralis are shaped by exploitation competition.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1918-1929"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive toxicity assessment of nanodiamond on Blaps polychresta: implications and novel findings. 纳米金刚石对 Blaps polychresta 的综合毒性评估：影响和新发现。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-26 DOI: 10.1111/1744-7917.13357

Marwa Saad, Nabila Selim, Lamia M El-Samad

{"title":"Comprehensive toxicity assessment of nanodiamond on Blaps polychresta: implications and novel findings.","authors":"Marwa Saad, Nabila Selim, Lamia M El-Samad","doi":"10.1111/1744-7917.13357","DOIUrl":"10.1111/1744-7917.13357","url":null,"abstract":"With the increasing development of nanomaterials, the use of nanodiamonds (NDs) has been broadly manifested in many applications. However, their high penetration into the ecosystem indubitably poses remarkable toxicological risks. This paper investigates the toxic effects of NDs on the darkling beetle, Blaps polychresta Forskal, 1775 (Coleoptera: Tenebrionidae). Survival analysis was carried out by monitoring the beetles for 30 d after the injection of four different doses of NDs. A dose of 10.0 mg NDs/g body weight, causing less than 50% mortality effect, was assigned in the analysis of the different organs of studied beetles, including testis, ovary, and midgut. Structural and ultrastructural analyses were followed using light, TEM, and SEM microscopes. In addition, a variety of stress markers and enzyme activities were assessed using spectrophotometric methods. Furthermore, cell viability and DNA damage were evaluated using cytometry and comet assay, respectively. Compared to the control group, the NDs-treated group was exposed to various abnormalities within all the studied organs as follows. Significant disturbances in enzyme activities were accompanied by an apparent dysregulation in the antioxidant system. The flow cytometry results indicated a substantial decrease of viable cells along with a rise of apoptotic and necrotic cells. The comet assay demonstrated a highly increased level of DNA damage. Likewise, histological analyses accentuated the same findings showing remarkable deformities in the studied organs. Prominently, the research findings substantially contribute for the first time to evaluating the critical effects of NDs on B. polychresta, adopted as the bioindicator in this paper.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1838-1863"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fasting as a precursor to high-fat diet enhances mitochondrial resilience in Drosophila melanogaster. 禁食是高脂饮食的前奏，可增强黑腹果蝇线粒体的恢复能力。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-21 DOI: 10.1111/1744-7917.13355

Florence Hunter-Manseau, Simon B Cormier, Rebekah Strang, Nicolas Pichaud

{"title":"Fasting as a precursor to high-fat diet enhances mitochondrial resilience in Drosophila melanogaster.","authors":"Florence Hunter-Manseau, Simon B Cormier, Rebekah Strang, Nicolas Pichaud","doi":"10.1111/1744-7917.13355","DOIUrl":"10.1111/1744-7917.13355","url":null,"abstract":"Changes in diet type and nutrient availability can impose significant environmental stress on organisms, potentially compromising physiological functions and reproductive success. In nature, dramatic fluctuations in dietary resources are often observed and adjustments to restore cellular homeostasis are crucial to survive this type of stress. In this study, we exposed male Drosophila melanogaster to two modulated dietary treatments: one without a fasting period before exposure to a high-fat diet and the other with a 24-h fasting period. We then investigated mitochondrial metabolism and molecular responses to these treatments. Exposure to a high-fat diet without a preceding fasting period resulted in disrupted mitochondrial respiration, notably at the level of complex I. On the other hand, a short fasting period before the high-fat diet maintained mitochondrial respiration. Generally, transcript abundance of genes associated with mitophagy, heat-shock proteins, mitochondrial biogenesis, and nutrient sensing pathways increased either slightly or significantly following a fasting period and remained stable when flies were subsequently put on a high-fat diet, whereas a drastic decrease of almost all transcript abundances was observed for all these pathways when flies were exposed directly to a high-fat diet. Moreover, mitochondrial enzymatic activities showed less variation after the fasting period than the treatment without a fasting period. Overall, our study sheds light on the mechanistic protective effects of fasting prior to a high-fat diet and highlights the metabolic flexibility of Drosophila mitochondria in response to abrupt dietary changes and have implication for adaptation of species to their changing environment.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1770-1788"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographic variation in larval cold tolerance and exposure across the invasion front of a widely established forest insect. 一种广泛传播的森林昆虫在入侵前线的幼虫耐寒性和暴露程度的地理差异。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-22 DOI: 10.1111/1744-7917.13358

Petra Hafker, Lily M Thompson, Jonathan A Walter, Dylan Parry, Kristine L Grayson

{"title":"Geographic variation in larval cold tolerance and exposure across the invasion front of a widely established forest insect.","authors":"Petra Hafker, Lily M Thompson, Jonathan A Walter, Dylan Parry, Kristine L Grayson","doi":"10.1111/1744-7917.13358","DOIUrl":"10.1111/1744-7917.13358","url":null,"abstract":"Under global climate change, high and low temperature extremes can drive shifts in species distributions. Across the range of a species, thermal tolerance is based on acclimatization, plasticity, and may undergo selection, shaping resilience to temperature stress. In this study, we measured variation in cold temperature tolerance of early instar larvae of an invasive forest insect, Lymantria dispar dispar L. (Lepidoptera: Erebidae), using populations sourced from a range of climates within the current introduced range in the Eastern United States. We tested for population differences in chill coma recovery (CCR) by measuring recovery time following a period of exposure to a nonlethal cold temperature in 2 cold exposure experiments. A 3rd experiment quantified growth responses after CCR to evaluate sublethal effects. Our results indicate that cold tolerance is linked to regional climate, with individuals from populations sourced from colder climates recovering faster from chill coma. While this geographic gradient is seen in many species, detecting this pattern is notable for an introduced species founded from a single point-source introduction. We demonstrate that the cold temperatures used in our experiments occur in nature during cold spells after spring egg hatch, but impacts to growth and survival appear low. We expect that population differences in cold temperature performance manifest more from differences in temperature-dependent growth than acute exposure. Evaluating intraspecific variation in cold tolerance increases our understanding of the role of climatic gradients on the physiology of an invasive species, and contributes to tools for predicting further expansion.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1930-1942"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The symbiont Wolbachia alleviates pesticide susceptibility in the two-spotted spider mite Tetranychus urticae through enhanced host detoxification pathways. Wolbachia 共生体通过增强宿主的解毒途径减轻二斑蜘蛛螨对杀虫剂的敏感性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-02-22 DOI: 10.1111/1744-7917.13341

Qing-Tong Ye, Xue Gong, Huan-Huan Liu, Bing-Xuan Wu, Chang-Wu Peng, Xiao-Yue Hong, Xiao-Li Bing

{"title":"The symbiont Wolbachia alleviates pesticide susceptibility in the two-spotted spider mite Tetranychus urticae through enhanced host detoxification pathways.","authors":"Qing-Tong Ye, Xue Gong, Huan-Huan Liu, Bing-Xuan Wu, Chang-Wu Peng, Xiao-Yue Hong, Xiao-Li Bing","doi":"10.1111/1744-7917.13341","DOIUrl":"10.1111/1744-7917.13341","url":null,"abstract":"The two-spotted spider mite (Tetranychus urticae) is one of the most well-known pesticide-resistant agricultural pests, with resistance often attributed to changes such as target-site mutations and detoxification activation. Recent studies show that pesticide resistance can also be influenced by symbionts, but their involvement in this process in spider mites remains uncertain. Here, we found that infection with Wolbachia, a well-known bacterial reproductive manipulator, significantly increased mite survival after exposure to the insecticides abamectin, cyflumetofen, and pyridaben. Wolbachia-infected (WI) mites showed higher expression of detoxification genes such as P450, glutathione-S-transferase (GST), ABC transporters, and carboxyl/cholinesterases. RNA interference experiments confirmed the role of the two above-mentioned detoxification genes, TuCYP392D2 and TuGSTd05, in pesticide resistance. Increased GST activities were also observed in abamectin-treated WI mites. In addition, when wild populations were treated with abamectin, WI mites generally showed better survival than uninfected mites. However, genetically homogeneous mites with different Wolbachia strains showed similar survival. Finally, abamectin treatment increased Wolbachia abundance without altering the mite's bacterial community. This finding highlights the role of Wolbachia in orchestrating pesticide resistance by modulating host detoxification. By unraveling the intricate interplay between symbionts and pesticide resistance, our study lays the groundwork for pioneering strategies to combat agricultural pests.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1822-1837"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139931018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

iSalivaomicDB: A comprehensive saliva omics database for insects. iSalivaomicDB：昆虫唾液全息数据库。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 Epub Date: 2024-03-07 DOI: 10.1111/1744-7917.13349

Bo Zhang, Longfei Chen, Shan Xiao, Cong Dang, Fang Wang, Qi Fang, Xinhai Ye, David W Stanley, Gongyin Ye

引用次数: 0

miRNA targeting Mpp53 is involved in UV-B irradiation resistance in Myzus persicae. 以Mpp53为靶点的miRNA参与了桃蚜抗UV-B辐射的过程。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-12-01 DOI: 10.1111/1744-7917.13472

Longchun He, Changli Yang, Jianyu Meng, Xue Tang, Changyu Zhang

{"title":"miRNA targeting Mpp53 is involved in UV-B irradiation resistance in Myzus persicae.","authors":"Longchun He, Changli Yang, Jianyu Meng, Xue Tang, Changyu Zhang","doi":"10.1111/1744-7917.13472","DOIUrl":"https://doi.org/10.1111/1744-7917.13472","url":null,"abstract":"Ultraviolet (UV) radiation, an environmental stressor, is crucial for the survival and adaptation of organisms. Myzus persicae, a global pest, is exposed to sunlight year-round, making it unable to avoid UV rays in its environment. MicroRNAs (miRNAs) are important posttranscriptional regulators of gene expression and mediate various biological processes. However, the role of miRNA in aphids in response to UV-B stress is unclear. In this study, Mpp53 expression level significantly increased with an increase in the duration of UV-B radiation, peaking at 2 h; knockdown of Mpp53 decreased the survival rate of aphids under UV-B stress, suggesting that Mpp53 is involved in aphid responses to UV-B. Here, we first predicted 8 miRNAs targeting Mpp53, and then screened for miRNAs related to UV-B resistance in aphids; of these, 5 miRNAs (miR-305-5p, novel_50, novel_80, novel_166, and novel_61) were found to target Mpp53. Luciferase reporter assays demonstrated that novel_61 binds to the noncoding region of Mpp53 and downregulates its expression. Overexpression of novel_61 in aphids decreased Mpp53 expression and caused significant mortality under UV-B irradiation. Furthermore, the aphids exhibited lower reproductive capacity, lower body weight, and shorter body length and width. This is the first study to systematically screen and identify miRNA related to aphid responses to UV-B stress and deepens our understanding of the molecular mechanism of insect responses to environmental stress, which may eventually aid in developing better control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0