Insect Science最新文献_第10页

CRISPR screening unravels genome-wide regulators of juvenile hormone signaling in Bombyx mori. CRISPR筛选揭示家蚕幼崽激素信号的全基因组调节因子。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-02 DOI: 10.1111/1744-7917.70103

Hao Sun, Chaojie Chen, Zijian Shi, Yue Liu, Ruolin Wang, Dan Liu, Tong Zhang, Sanyuan Ma, Ling Jia

{"title":"CRISPR screening unravels genome-wide regulators of juvenile hormone signaling in Bombyx mori.","authors":"Hao Sun, Chaojie Chen, Zijian Shi, Yue Liu, Ruolin Wang, Dan Liu, Tong Zhang, Sanyuan Ma, Ling Jia","doi":"10.1111/1744-7917.70103","DOIUrl":"https://doi.org/10.1111/1744-7917.70103","url":null,"abstract":"Insect metamorphosis constitutes a sophisticated endocrine cascade governed by the dynamic interplay between juvenile hormone (JH) and 20-hydroxyecdysone (20E). Although JH has been established as a critical developmental regulator, its intracellular signaling mechanisms remain incompletely characterized. In this study, we implemented a genome-wide CRISPR screening strategy in the lepidopteran model Bombyx mori to systematically identify components of JH signaling. First, we developed a JH-responsive reporter cell line with EGFP expression under the control of JH-sensitive promoters, enabling real-time monitoring of pathway activation. Then we established a genome-scale CRISPR knockout library specifically optimized for silkworm cells, and performed screening through integration of high-throughput flow cytometry and next-generation sequencing. Systematic analysis revealed a total of 387 candidate genes that significantly modulated JH signaling activity (231 enriched vs. 156 depleted). Functional annotation clustering revealed predominant enrichment in membrane biogenesis processes and small molecule metabolism. It is worth noting that through integrated genomic screening and experimental verification, we found that the tyrosine kinase receptor BmALK is an important regulatory node for JH signal transduction. This study establishes the first comprehensive genetic landscape of JH signaling in a holometabolous insect, providing a robust platform for functional mining of JH signaling pathways, and molecular evidence for membrane-associated JH signal transduction in Lepidoptera.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bumblebees prefer shorter over longer strings and connected over disconnected ones in string-pulling tasks but prioritize connectivity over spatial proximity when the two are in conflict. 在拉绳任务中，大黄蜂更喜欢短的绳子而不是长绳子，更喜欢连接的绳子而不是断开的绳子，但当两者冲突时，它们会优先考虑连通性而不是空间接近性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-02 DOI: 10.1111/1744-7917.70113

Chao Wen, Shunping Dong, Yuhao Guo, Cai Wang, Weicheng Ding, Jin Ge, Junbao Wen, Lars Chittka

{"title":"Bumblebees prefer shorter over longer strings and connected over disconnected ones in string-pulling tasks but prioritize connectivity over spatial proximity when the two are in conflict.","authors":"Chao Wen, Shunping Dong, Yuhao Guo, Cai Wang, Weicheng Ding, Jin Ge, Junbao Wen, Lars Chittka","doi":"10.1111/1744-7917.70113","DOIUrl":"https://doi.org/10.1111/1744-7917.70113","url":null,"abstract":"We aimed to investigate the trade-off strategies between efficiency and accessibility employed by bumblebees (Bombus terrestris) when solving string-pulling tasks. In these tasks, bees were required to choose between two strings, which varied in length, and whether or not they were connected to an artificial flower. Our results indicate that when presented with strings of varying lengths connected to artificial flowers containing rewards, the bees consistently preferred the shorter string. Instead of learning the string length experienced during training, the bees showed a preference for the shorter string, even after being trained with a longer one. Bumblebees were also capable of selecting connected over disconnected strings, even when proximity served as a confounding factor. These findings demonstrate that bumblebees flexibly deploy a diversity of strategies to solve string-pulling tasks, shedding light on their problem-solving capabilities when tackling non-natural spatial and object manipulation tasks.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissecting the roles of Taiman in pupation and female reproduction of Helicoverpa armigera. 台湾人在棉铃虫化蛹和雌性繁殖中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-30 DOI: 10.1111/1744-7917.70096

Long Ma, Hao-Jie Zheng, Hong-Bin Ge, Ying-Chuan Peng, You-Xin Yang, Zi-Wei Zhao, Wan-Na Zhang, Hai-Jun Xiao

{"title":"Dissecting the roles of Taiman in pupation and female reproduction of Helicoverpa armigera.","authors":"Long Ma, Hao-Jie Zheng, Hong-Bin Ge, Ying-Chuan Peng, You-Xin Yang, Zi-Wei Zhao, Wan-Na Zhang, Hai-Jun Xiao","doi":"10.1111/1744-7917.70096","DOIUrl":"https://doi.org/10.1111/1744-7917.70096","url":null,"abstract":"In insects, metamorphosis and reproduction are coordinated by juvenile hormone (JH) and 20-hydroxyecdysone (20E). A transcription factor Taiman (Tai) serves as a coactivator of the functional 20E receptor and heterodimerizes with JH receptor Methoprene-tolerant (Met) to form an active receptor complex, encoding a key modulator of JH and 20E signaling pathways. However, direct evidence for the participation of Tai in metamorphic actions and vitellogenesis is quite limited. Herein, 1 isoform of Tai was identified from Helicoverpa armigera, named HaTai; the transcription of HaTai was stimulated by either JH or 20E ingestion. Knocking down HaTai in 5th instar larvae caused severe pupation failure and weight loss, wherein 69.44% of HaTai-RNAi (RNA interference) larvae died from pupation failure within 6 d. The HaTai-RNAi larvae exhibited attenuated JH signaling but intensified ecdysteroidogenesis, whereas supplementation with JH or 20E failed to recover the expression of HaTai. In female adults, depleting HaTai blocked vitellogenin transcription and caused atrophied ovaries with deficient yolk protein deposition. The HaTai-depleted females exhibited a substantial decrease in egg production. Moreover, the HaTai-depleted fat body became remarkably loose and partly vacuolated, accompanied by a reduction of triglyceride content. To explore the feasibility of oral-ingested RNAi, feeding chitosan-wrapped double-stranded RNA targeting Tai induced a pronounced silencing effect in larvae, causing a significant increase in larval mortality and a decrease in pupation rate. Our study therefore unveiled the complex roles of HaTai in pupation and JH-mediated vitellogenesis, and explored an effective RNAi method applicable in H. armigera.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The JAK-STAT pathway in invertebrates: An emerging battleground for host‒virus warfare. 无脊椎动物的JAK-STAT通路：宿主-病毒战争的新战场

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-30 DOI: 10.1111/1744-7917.70109

Hengchuan Xia, Cong Zhang, Zhongjian Guo, Liang Chen, Keping Chen

{"title":"The JAK-STAT pathway in invertebrates: An emerging battleground for host‒virus warfare.","authors":"Hengchuan Xia, Cong Zhang, Zhongjian Guo, Liang Chen, Keping Chen","doi":"10.1111/1744-7917.70109","DOIUrl":"https://doi.org/10.1111/1744-7917.70109","url":null,"abstract":"The arms race between hosts and viruses for dominance over the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway has been extensively studied in vertebrates but remains poorly explored in invertebrates. Recent studies have revealed that the antiviral Vago/JAK-STAT pathway in invertebrates operates as a parallel mechanism to the vertebrate interferon (IFN)/JAK-STAT pathway, suggesting a highly conserved antiviral mechanism across the animal kingdom. Moreover, invertebrates appear to employ multifaceted strategies to modulate the JAK-STAT pathway to inhibit viral replication or enhance host tolerance, while viruses can counteract by manipulating the JAK-STAT pathway to their advantage. Thus, the competition between hosts and viruses for control of the JAK-STAT pathway also exists in invertebrates, indicating this arms race is also conserved in evolution. This review summarizes the discovery of the Vago/JAK-STAT pathway, its comparison to the IFN/JAK-STAT pathway, and the mechanisms underlying the host-virus tug-of-war over the JAK-STAT pathway in invertebrates. These insights shed light on this highly conserved immune strategy, its implication for host-virus interaction, and its potential application in disease control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of pig manure-derived gut microbiota in accelerating the growth and development of Sarcophaga africa. 猪粪便来源的肠道微生物群在加速非洲石棺菌生长和发育中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-30 DOI: 10.1111/1744-7917.70104

Fengqin Yang, Xiangyan Zhang, Yanjie Shang, Jiao Xiao, Jian Zhao, Xingchun Zhao, Yadong Guo

{"title":"The role of pig manure-derived gut microbiota in accelerating the growth and development of Sarcophaga africa.","authors":"Fengqin Yang, Xiangyan Zhang, Yanjie Shang, Jiao Xiao, Jian Zhao, Xingchun Zhao, Yadong Guo","doi":"10.1111/1744-7917.70104","DOIUrl":"https://doi.org/10.1111/1744-7917.70104","url":null,"abstract":"Exploring the impact of diet-derived gut microbiota on insect growth and development holds significant value not only for understanding the \"diet-gut microbes-insects\" interactions, but also for forensic entomology, as it lays the research foundation for clarifying the relationships among \"cadavers-microbes-necrophagous insects\". This study investigates the effects of pig manure-derived gut microbiota on the growth and development of Sarcophaga africa, a key necrophagous insect. Our findings demonstrate that, compared to pig lung, pig manure significantly increased larval amount without affecting adult eclosion rates, thereby facilitating laboratory population establishment and accelerating development. Through microbial sequencing, key target bacteria were identified and validated using quantitative detection and antibiotic interference experiments. The results highlight two critical aspects: first, dietary microbes play a central role in shaping the gut microbial community structure of larvae; second, gut microbes derived from pig manure, particularly Clostridium_sensu_stricto_1, may enhance the developmental process of S. africa by directly increasing protein levels in 3rd instar larvae and indirectly reducing glycogen levels in late pupae. Additionally, developmental data for S. africa at 30 °C were obtained to establish reference values for forensic applications using necrophagous insects in postmortem interval estimation. These findings highlight the need for forensic entomologists to consider how microbial-driven developmental variations may affect the reliability of developmental data in such studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mating affects dopamine signaling in Drosophila melanogaster females. 交配影响雌性黑腹果蝇的多巴胺信号。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-29 DOI: 10.1111/1744-7917.70112

Elena V Burdina, Margarita A Bobrovskikh, Natalya V Adonyeva, Alexander A Alekseev, Olga V Andreenkova, Maksim A Deryuzhenko, Olga D Shishkina, Evgenia K Karpova, Nataly E Gruntenko

引用次数: 0

The role of dopaminergic neuronal clusters in governing division of labor in ants. 多巴胺能神经元簇在蚂蚁劳动分工中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-27 DOI: 10.1111/1744-7917.70087

Wenjiang Zhong, Nianxia Xie, Guo Ding, Jie Zhao, Pei Zhang, Qiye Li, Hao Ran, Guojie Zhang, Weiwei Liu

{"title":"The role of dopaminergic neuronal clusters in governing division of labor in ants.","authors":"Wenjiang Zhong, Nianxia Xie, Guo Ding, Jie Zhao, Pei Zhang, Qiye Li, Hao Ran, Guojie Zhang, Weiwei Liu","doi":"10.1111/1744-7917.70087","DOIUrl":"https://doi.org/10.1111/1744-7917.70087","url":null,"abstract":"Reproductive division of labor is one of the most prominent features of social insects. Yet, the neural mechanisms that govern this division and the associated behavioral differentiation among castes remain obscure. In this study, we systematically characterized the anatomical features of dopamine neurons in the brain of Monomorium pharaonis and compared the cell number and spatial distribution of these neurons across castes. We identified 17 anatomically distinct clusters of dopamine neurons in the ant brain, with cell numbers varying from ∼322 to ∼431 across castes. The major dopamine clusters are located in brain regions analogous to those in flies, with 2 clusters, PAL and PPL1 exhibiting significantly higher cell numbers in ants than in flies. Notably, 4 clusters, DAM, D1, DPL, and PPL2, showed remarkable variation in cell numbers across castes. Using single-cell transcriptomics, we identified specific molecular markers for subdividing dopamine neurons. We validated the expression of multiple neuropeptide genes in specific dopamine clusters. In particular, we found that PPL2 cluster can be further divided into 2 subclusters, PPL2a and PPL2b, which are partially labeled by the peptide gene Nplp1. PPL2b neurons, characterized by larger cell bodies, and present only in unmated queens and males, are absent in mature queens and workers. These neurons are located adjacent to Nlg2-expressing lobular neurons, which are also absent in workers and may play a role in regulating mating behaviors. Our findings provide a foundation for further investigation into the neural mechanism underlying division of labor and caste-specific behaviors in ant.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bi-environmental cage for colony management in the mass rearing of Anastrepha ludens (Diptera: Tephritidae). 双环境网箱在大规模饲养小绒蚤中的群体管理（双翅目：绒蚤科）。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-27 DOI: 10.1111/1744-7917.70105

José S Meza, Jorge Ibañez-Palacios, Daisy P Cardenas-Enriquez, Juan H Luis-Alvares, Pablo Liedo

{"title":"Bi-environmental cage for colony management in the mass rearing of Anastrepha ludens (Diptera: Tephritidae).","authors":"José S Meza, Jorge Ibañez-Palacios, Daisy P Cardenas-Enriquez, Juan H Luis-Alvares, Pablo Liedo","doi":"10.1111/1744-7917.70105","DOIUrl":"https://doi.org/10.1111/1744-7917.70105","url":null,"abstract":"Anastrepha ludens (Loew) is controlled in Mexico using sterile insect technique (SIT). SIT relies primarily on mass-reared insects, which are subjected to the effects of selection during colonization and rearing, and that frequently result in modifications of their biology and behavior. Here, we propose and evaluate a novel \"bi-environmental cage\" for colony management which promotes more natural sexual selection. The cage allows each sex to reach sexual maturity in separate compartments acclimatized according to natural conditions. Females mature in areas where they can recognize oviposition sites, while males mature in areas populated with small trees to allow establishment of territories in leks and performance of courtship behaviors. To determine whether the bi-environmental cage can minimize the potential adverse effects on mating competitiveness, two strains of A. ludens were tested; wild flies strain and genetic sexing strain Tapachula 7 (mass-reared flies). We found that after 4 generations in the mass-reared flies in the bi-environmental cage showed a level of fecundity similar to that of flies from the conventional cage. A similar pattern was also seen in the case of wild flies in both types of cages. In addition, other biological attributes of the wild strain assessed over six generations showed adaptability to mass-rearing conditions. Wild males from the bi-environmental cages were more sexually competitive than those from the conventional cage. Our results show that it is possible to mitigate many of the detrimental effects of domestication on the sexual performance of mass-reared males by using close-to-natural conditions for colony management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic bases and robustness of the toxicological response to spinosad and lambda-cyhalothrin in Drosophila melanogaster. 黑胃果蝇对spinosad和lambda- cyhalthrin毒理学反应的遗传基础和稳健性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-27 DOI: 10.1111/1744-7917.70107

Mariana C Sabio, Raúl Adolfo Alzogaray, Madelein S M Ortiz, Juan José Fanara

{"title":"Genetic bases and robustness of the toxicological response to spinosad and lambda-cyhalothrin in Drosophila melanogaster.","authors":"Mariana C Sabio, Raúl Adolfo Alzogaray, Madelein S M Ortiz, Juan José Fanara","doi":"10.1111/1744-7917.70107","DOIUrl":"https://doi.org/10.1111/1744-7917.70107","url":null,"abstract":"Insecticides are valuable and widely used tools for the control of insect pests. However, we know very little about the genes and processes involved in the key steps of the poisoning sequence. Besides, the excessive and indiscriminate use of insecticides is generating mechanisms of resistance in various insect pests. To improve our understanding of insecticide toxicity, we need a powerful model organism to help describe the processes underlying insecticide poisoning and the use of genomic tools to identify and analyze the genetic basis of the toxicological response of the insecticides. In this context, we associate variation in toxicological response with genomic variation, to identify genetic polymorphisms underlying the different steps of the insect (genotype)-response (phenotype)-insecticide (environment) interaction. We investigate the genetic factors involved in the toxicological response of D. melanogaster lines when exposed to spinosad and lambda-cyhalothrin through the measured of KT50 in adult males. Since the genomes of all lines evaluated are completely sequenced, we performed a Genome Wide Association Study that enabled us to identify genetic polymorphisms and candidate genes responsible for the overall phenotypic variation. Most of the candidate genes detected exhibited insecticide-specific effect and play roles in the toxicodynamics as AstC-R1, Dh44-R, stan, Ca-β, AgmNAT, Acox57D-d, Btk, CarT, dpr8, Pkd2, and Shab and the toxicokinetics like for example ckd, Hr38, robo2, Toll-4, Eglp2, and Prip of spinosad and lambda-cyhalothrin. Finally our results suggest that in the case of lambda-cyhalothrin genotypes exhibiting resistance phenotypes (higher KT50) also displaying a less constant (robust) response.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dilemma of honey bee pest management in European Union: eradication or coexistence? 欧盟蜜蜂病虫害管理的困境：根除还是共存？

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70097

Michela Bertola, Franco Mutinelli

引用次数: 0