Insect Science最新文献_第10页

Development of the insect adult fat body relies on glycolysis, lipid synthesis, cell proliferation, and cell adhesion. 昆虫成虫脂肪体的发育依赖于糖酵解、脂质合成、细胞增殖和细胞粘附。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-26 DOI: 10.1111/1744-7917.13438

Ke-Yan Jin, Yu-Qin Di, Tian-Wen Liu, Xiao-Fan Zhao

{"title":"Development of the insect adult fat body relies on glycolysis, lipid synthesis, cell proliferation, and cell adhesion.","authors":"Ke-Yan Jin, Yu-Qin Di, Tian-Wen Liu, Xiao-Fan Zhao","doi":"10.1111/1744-7917.13438","DOIUrl":"https://doi.org/10.1111/1744-7917.13438","url":null,"abstract":"The fat body of the holometabolous insect is remodeled by the degradation of the larval fat body and the development of the adult fat body during metamorphosis. However, the mechanism of adult fat body development is quite unclear. Using the agricultural pest Helicoverpa armigera, the cotton bollworm, as a model, we revealed that the development of adult fat body was regulated by glycolysis, triglyceride (triacylglycerol [TAG]) synthesis, cell proliferation, and cell adhesion. RNA sequencing detected a set of genes that were upregulated in the 8-d late pupal fat body at a late metamorphic stage compared with the 2-d pupal fat body at an earlier metamorphic stage. The pathways for glycolysis, TAG synthesis, cell proliferation, and cell adhesion were enriched by the differentially expressed genes, and the key genes linked with these pathways showed increased expression in the 8-d pupal fat body. Knockdown of phosphofructokinase (Pfk), acetyl-CoA carboxylase (Acc1), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit (P110) and collagen alpha-1(IV) chain (Col4a1) by RNA interference resulted in abnormal eclosion and death at pupal stages, and repressed lipid droplets accumulation and adult fat body development. The expression of Acc1, P110, and Col4a1 was repressed by the insect steroid hormone 20-hydroxyecdysone (20E). The critical genes in the 20E pathway appeared to decrease at the late pupal stage. These data suggested that the development of the insect adult fat body is regulated by glycolysis, lipids synthesis, cell proliferation, and cell adhesion at the late pupal stage when the 20E signal decreases.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensing volatiles throughout the body: geographic- and tissue-specific olfactory receptor expression in the fig wasp. 感知全身的挥发性物质：无花果蜂嗅觉受体的地理和组织特异性表达。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-25 DOI: 10.1111/1744-7917.13441

Sushma Krishnan, Snehal Dilip Karpe, Hithesh Kumar, Lucy B Nongbri, Vignesh Venkateswaran, Ramanathan Sowdhamini, Ewald Grosse-Wilde, Bill S Hansson, Renee M Borges

{"title":"Sensing volatiles throughout the body: geographic- and tissue-specific olfactory receptor expression in the fig wasp.","authors":"Sushma Krishnan, Snehal Dilip Karpe, Hithesh Kumar, Lucy B Nongbri, Vignesh Venkateswaran, Ramanathan Sowdhamini, Ewald Grosse-Wilde, Bill S Hansson, Renee M Borges","doi":"10.1111/1744-7917.13441","DOIUrl":"https://doi.org/10.1111/1744-7917.13441","url":null,"abstract":"An essential adaptive strategy in insects is the evolution of olfactory receptors (ORs) to recognize important volatile environmental chemical cues. Our model species, Ceratosolen fusciceps, a specialist wasp pollinator of Ficus racemosa, likely possesses an OR repertoire that allows it to distinguish fig-specific volatiles in highly variable environments. Using a newly assembled genome-guided transcriptome, we annotated 63 ORs in the species and reconstructed the phylogeny of Ceratosolen ORs in conjunction with other hymenopteran species. Expression analysis showed that though ORs were mainly expressed in the female antennae, 20% were also expressed in nonantennal tissues such as the head, thorax, abdomen, legs, wings, and ovipositor. Specific upregulated expression was observed in OR30C in the head and OR60C in the wings. We identified OR expression from all major body parts of female C. fusciceps, suggesting novel roles of ORs throughout the body. Further examination of the OR expression of C. fusciceps in widely separated geographical locations, that is, South (urban) and Northeast (rural) India, revealed distinct OR expression levels in different locations. This discrepancy likely parallels the observed variation in fig volatiles between these regions and provides new insights into the evolution of insect ORs and their expression across geographical locations and tissues.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entomopathogenic Bacillus cereus impairs the fitness of the spotted-wing drosophila, Drosophila suzukii. 昆虫病原蜡样芽孢杆菌损害斑翅果蝇的适应性

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-21 DOI: 10.1111/1744-7917.13439

Nana He, Shaojie Zhou, Chuanming Zhou, Weikang Yang, Sheng Zhang, Deqiang Yan, Xiaowen Ji, Wei Liu

{"title":"Entomopathogenic Bacillus cereus impairs the fitness of the spotted-wing drosophila, Drosophila suzukii.","authors":"Nana He, Shaojie Zhou, Chuanming Zhou, Weikang Yang, Sheng Zhang, Deqiang Yan, Xiaowen Ji, Wei Liu","doi":"10.1111/1744-7917.13439","DOIUrl":"https://doi.org/10.1111/1744-7917.13439","url":null,"abstract":"Drosophila suzukii is a notorious pest which causes devastating damage to thin-skinned fruits, and the larvae feed on the fruit, causing extensive agricultural economic loss. The current application of insecticides to manage this pest results in serious resistance and environmental hazards, so an alternative strategy for D. suzukii biocontrol is urgently needed. Here, we reported that entomopathogenic Bacillus cereus has the potential to biocontrol D. suzukii. We isolated and identified the bacterial strain, B. cereus H1, that was detrimental to the fitness of both D. suzukii progenies and parents. D. suzukii was robustly repelled to depositing eggs on the halves with metabolites of B. cereus H1. Both males and females of D. suzukii were susceptible to B. cereus H1. B. cereus H1 significantly arrested larval development with at least 40% lethal larvae. The median lethal time (LT50) of males and females of D. suzukii challenged with B. cereus H1 was 3 and 2 d, respectively. Moreover, B. cereus H1 disrupted the intestinal integrity and pH value of D. suzukii and resulted in an increase in bacterial load of guts and hemolymph. Mechanistically, infection of B. cereus H1 led to the activation of the dual oxidase (DUOX)-ROS-Jun N-terminal kinase (JNK) pathway. The findings showed that the entomopathogen B. cereus H1 could potentially act as a biological control agent against D. suzukii, advancing fundamental concepts of integrated pest management programs against D. suzukii.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disrupting the olfactory response in Monochamus saltuarius: Two potential target genes for controlling the vector insect of pine wilt disease. 破坏盐袋木虱的嗅觉反应：控制松树枯萎病病媒昆虫的两个潜在目标基因

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-20 DOI: 10.1111/1744-7917.13431

Rong Zhang, Jue Wang, Yanlong Zhang, Xizhuo Wang, Zhen Zhang, Xiangbo Kong, Fu Liu, Jiaxing Fang, Yanan Zheng, Sufang Zhang

{"title":"Disrupting the olfactory response in Monochamus saltuarius: Two potential target genes for controlling the vector insect of pine wilt disease.","authors":"Rong Zhang, Jue Wang, Yanlong Zhang, Xizhuo Wang, Zhen Zhang, Xiangbo Kong, Fu Liu, Jiaxing Fang, Yanan Zheng, Sufang Zhang","doi":"10.1111/1744-7917.13431","DOIUrl":"https://doi.org/10.1111/1744-7917.13431","url":null,"abstract":"Worldwide, pine forests have been threatened by a devastating pine wood disease caused by Bursaphelenchus xylophilus, with Monochamus saltuarius being a newly recorded vector of the disease in Northeast China. The olfactory system plays important roles in both feeding and oviposition during the adult stage of M. saltuarius, and olfactory gene function research is essential for gaining an understanding of the olfactory mechanisms of this pest. However, there is limited information available regarding olfactory gene functions in this pest. In the present study, we selected 7 olfactory genes, including 2 chemosensory proteins, 2 odorant-binding proteins, the odorant co-receptor and 2 odorant receptors, which were relatively highly expressed during the adult stage. We silenced these genes by RNA interference (RNAi), and real-time quantitative PCR was used to detect their expression levels after double-stranded RNA (dsRNA) injection. The results indicate that these genes were significantly downregulated at 2 d post-dsRNA injection, and this was sustained until 5 d post-injection. Electroantennography tests indicated that the knockdown of MsalOBP14 and MsalOrco impaired the olfactory response of M. saltuarius to 11 host volatiles and 1 sex pheromone compound. Y-tube experiments further confirmed that downregulated MsalOBP14 and MsalOrco expression led to olfactory dysfunction in M. saltuarius, which significantly lost selectivity. The results indicate that MsalOBP14 and MsalOrco play critical roles in sex communication and host volatile detection in M. saltuarius, and possibly represent 2 effective targets for controlling this forest pest through olfactory disruption.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of eicosanoids in insect immunity: new insights and recent advances. 二十烷酸在昆虫免疫中的作用：新见解和最新进展。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-19 DOI: 10.1111/1744-7917.13434

Shubhranil Brahma, Somnath Chatterjee, Atrayee Dey

{"title":"Role of eicosanoids in insect immunity: new insights and recent advances.","authors":"Shubhranil Brahma, Somnath Chatterjee, Atrayee Dey","doi":"10.1111/1744-7917.13434","DOIUrl":"https://doi.org/10.1111/1744-7917.13434","url":null,"abstract":"Viruses, bacteria, fungus, protozoans, and different metazoan parasites and parasitoids present a constant threat to insects. Insect immunity has two components: humoral and cell mediated. Humoral immunity can be achieved by various antimicrobial proteins, namely, cecropins, sarcotoxin, defensin, attacin, etc. The cell-mediated immunity comprises various cells having immune functions fostering nodulation, phagocytosis, microaggregation, encapsulation etc. Eicosanoids play a crucial role in insect immunity comparable to other animals. The above-mentioned are signaling molecules derived from polyunsaturated fatty acids and they exert numerous physiological effects, namely, inflammation, immune modulation, and regulation of cellular processes. The review article elucidates various roles of eicosanoids, namely, nodulation reaction, Toll signaling pathway, nitric oxide (NO) generation, Ca2+ mobilization, production of reactive oxygen species (ROS), actin polymerization and aquaporin activation. Eicosanoids can function in immune priming in insects drawing hemocytes. An agent named Duox was also identified serving as ROS generator in insect gut. Moreover, role of Repat gene in insect immunity was also studied. However, recently the role of prostacyclin (PGI2) was found to be negative as it inhibits platelet aggregation. In this brief review, we have tried to shed light on the various functions of eicosanoids in immunity of insect those have been discovered recently. This concise study will allow to decipher eicosanoids' function in insect immunity in a nutshell, and it will pave the way for more researches to understand the key players of insect immunity which may eventually help to develop novel vector and pest control strategies in near future.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The odorant-binding protein genes obp67 and obp56d-like encode products that guide oviposition site selection in the Asian tiger mosquito, Aedes albopictus. 气味结合蛋白基因obp67和obp56d-like编码的产物可指导白纹伊蚊选择产卵地点。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-12 DOI: 10.1111/1744-7917.13430

Siyu Zhao, Hongkai Liu, Yiming Wu, Peilin Wu, Junyu Fu, Huijuan Yang, Anthony A James, Xiao-Guang Chen

{"title":"The odorant-binding protein genes obp67 and obp56d-like encode products that guide oviposition site selection in the Asian tiger mosquito, Aedes albopictus.","authors":"Siyu Zhao, Hongkai Liu, Yiming Wu, Peilin Wu, Junyu Fu, Huijuan Yang, Anthony A James, Xiao-Guang Chen","doi":"10.1111/1744-7917.13430","DOIUrl":"https://doi.org/10.1111/1744-7917.13430","url":null,"abstract":"Aedes albopictus is an important vector of arboviruses and prefers small containers of stagnant water as oviposition sites. One of the mechanisms mosquitoes use to search for suitable oviposition sites is relying on odor cues from prospective sites and their surroundings. The genetic and molecular bases of this behavior are not known for Ae. albopictus. Oviposition site-searching behavior can be separated into 2 stages: container location and water detection. We applied a glue compound to the antennae and the maxillary palps of adult females to mask their ability to detect molecules that may guide them to preferred oviposition sites. Treatment of the antennae significantly reduces the location index (P < 0.001), indicating a decreased ability to find oviposition sites, whereas no significant difference was observed in mosquitoes with maxillary palps treated with the same glue compound (P > 0.05). The detection time, measured as the duration from contact with the water surface to the deposition of the first egg, was extended in mosquitoes with treated antennae or maxillary palps, supporting the conclusion that olfaction is involved in the detection of oviposition site. Transcriptomic analysis identified differentially expressed olfactory-related genes, including obp67, obp56d-like, obp19d-like and obp67-like. RNA interference (RNAi)-mediated knockdown of obp67 and obp56d-like significantly affected the location index and detection time, respectively. Cas9/guide RNA-mediated knockout of obp56d-like resulted in a prolonged detection time, compared with the wild type (P < 0.05). These findings help to elucidate aspects of the olfactory mechanisms involved in Ae. albopictus oviposition site selection, and provide a basis for the development of mosquito surveillance and control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A long cold storage protocol for Anisopteromalus calandrae based on promoting prepupal development and arresting early pupal growth under low temperature. 基于在低温条件下促进蛹前期发育和抑制蛹早期生长的卡兰德拉蝶（Anisopteromalus calandrae）长期冷藏方案。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-12 DOI: 10.1111/1744-7917.13436

Jiapeng Yang, Yue Zhang, Li Liu, Die Liu, Guy Smagghe, Maofa Yang, Xiaoyulong Chen, Hong Yang, Renhuai Dai

{"title":"A long cold storage protocol for Anisopteromalus calandrae based on promoting prepupal development and arresting early pupal growth under low temperature.","authors":"Jiapeng Yang, Yue Zhang, Li Liu, Die Liu, Guy Smagghe, Maofa Yang, Xiaoyulong Chen, Hong Yang, Renhuai Dai","doi":"10.1111/1744-7917.13436","DOIUrl":"https://doi.org/10.1111/1744-7917.13436","url":null,"abstract":"Anisopteromalus calandrae (Howard) shows great promise as an ectoparasitoid for controlling various coleopteran pests in warehouses. However, for a large-scale release, it is crucial to establish an ample supply of A. calandrae while carefully maintaining their quality and effectiveness. Appropriate cold storage techniques are the key to achieving these goals. Previous studies on cold storage have focused on specific developmental stages and explored cold storage conditions that can be applied only to those stages. Herein, we examined the development, survival and reproductive capacity of A. calandrae at different temperatures (13, 16, and 19 °C) and storage durations (30, 60, and 90 d) and evaluated the fitness of the offspring. A. calandrae completed its egg-to-larva development and pupated at 16 °C, but its development was arrested at an early pupal stage. Even after 90 d of cold storage at 16 °C, the survival rate of A. calandrae remained high at 77%, with no significant impact on reproductive capacity. Furthermore, cold storage showed no negative effect on the F1 generation. In contrast, eggs stored at 13 °C failed to hatch, whereas those stored at 19 °C developed. Adults emerged after > 60 d. This indicates that storage at 19 °C is only suitable for short durations. Our findings highlight the developmental pattern of A. calandrae at 16 °C, indicating that the parasitic wasp can be stored for a long time at this temperature across all stages of development before pupation, substantially facilitating its mass reproduction and industrial production.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of integrins in cellular immunity of the oriental armyworm, Mythimna separata. 东方军蝽（Mythimna separata）细胞免疫中整合素的特征。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-12 DOI: 10.1111/1744-7917.13432

Yuting Mao, Seiichi Furukawa

{"title":"Characterization of integrins in cellular immunity of the oriental armyworm, Mythimna separata.","authors":"Yuting Mao, Seiichi Furukawa","doi":"10.1111/1744-7917.13432","DOIUrl":"https://doi.org/10.1111/1744-7917.13432","url":null,"abstract":"Insect hemocytes eliminate foreign substances from the hemocoel through various immune reactions. Integrins, receptor proteins present on the cell membrane, are formed as a heterodimer from α and β subunits and are known to be involved in various immune reactions. To elucidate the role of integrins in the immunity of the lepidoptera Mythimna separata, genes encoding integrins were screened from the genome, resulting in the identification of eight α and four β integrin genes. The expression levels of the integrin genes did not change in response to the injection of small abiotic beads undergoing phagocytosis in M. separata larvae. However, significant inductions of some integrin gene expressions were observed in hemocytes that formed capsules around large abiotic beads during encapsulation, especially in MysIntα2. Under biotic stimulation, induction of the MysIntα2 was evident after exposures to Gram-negative bacteria (Escherichia coli) and entomopathogenic nematodes (Steinernema carpocapsae), but not to Gram-positive bacteria (Micrococcus luteus). Immunostaining analysis revealed that MysIntα2 was specifically localized to hemocytes surrounding the beads during the encapsulation reaction. Furthermore, the spreading and encapsulation abilities of hemocytes were significantly inhibited by incubation with MysIntα2 antibodies. Suppression of MysIntα2 expression in M. separata larvae by injecting double-stranded RNA also resulted in a decrease in encapsulation activity. Collectively, these results indicate that MysIntα2 plays pivotal roles in the cellular immune response of M. separata, particularly during encapsulation. This likely occurs through the regulation of hemocyte spreading activity, thereby facilitating the formation of multilayered capsules around large invaders.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neighbors sharing pathogens: the intricate relationship between Apis mellifera and ants (Hymenoptera: Formicidae) nesting in hives. 共享病原体的邻居：蜂巢中筑巢的蜜蜂和蚂蚁（膜翅目：蚁科）之间错综复杂的关系。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-09 DOI: 10.1111/1744-7917.13433

Rossella Tiritelli, Daniele Giannetti, Enrico Schifani, Donato A Grasso, Giovanni Cilia

{"title":"Neighbors sharing pathogens: the intricate relationship between Apis mellifera and ants (Hymenoptera: Formicidae) nesting in hives.","authors":"Rossella Tiritelli, Daniele Giannetti, Enrico Schifani, Donato A Grasso, Giovanni Cilia","doi":"10.1111/1744-7917.13433","DOIUrl":"https://doi.org/10.1111/1744-7917.13433","url":null,"abstract":"Ants are ubiquitous and eusocial insects that exhibit frequent physical contact among colony members, thereby increasing their susceptibility to diseases. Some species are often found in beehives and in their surroundings, where they exploit the food resources of honey bees. This intricate relationship may facilitate the interspecific transmission of honey bee pathogens to ants, although ants themselves may contribute to spillback phenomena. The objective of this study was to assess the presence and abundance of honey bee pathogens in ants sampled from Italian apiaries. A total of 37 colonies within 24 apiaries across 7 regions were monitored. In total, 6 pathogens were detected in adult ants and 3 in the brood. In particular, the study revealed a high prevalence of honey bee pathogens in ants, with DWV, BQCV, and CBPV being the most commonly encountered. The brood also tested positive for the same viruses. Notably, all analyzed viruses were found to be replicative in both adult ants and ant broods. Furthermore, co-infections were prevalent, suggesting complex pathogen interactions within ant populations. Statistical analysis indicated significant differences in pathogen prevalence and abundance among ant species and sample types. The findings highlight active infection in both the ants and the brood, suggesting a potential role of ants as reservoir hosts and vectors of honey bee pathogens emphasizing the need for further research to understand the implications of interspecific pathogen transmission on ant and bee health.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for transient deleterious thermal acclimation in field recapture rates of an invasive tropical species, Bactrocera dorsalis (Diptera: Tephritidae). 热带入侵物种 Bactrocera dorsalis（双翅目：Tephritidae）野外再捕获率中瞬时有害热适应的证据。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-09 DOI: 10.1111/1744-7917.13435

Kevin Malod, Anandi Bierman, Minette Karsten, Aruna Manrakhan, Christopher W Weldon, John S Terblanche

{"title":"Evidence for transient deleterious thermal acclimation in field recapture rates of an invasive tropical species, Bactrocera dorsalis (Diptera: Tephritidae).","authors":"Kevin Malod, Anandi Bierman, Minette Karsten, Aruna Manrakhan, Christopher W Weldon, John S Terblanche","doi":"10.1111/1744-7917.13435","DOIUrl":"https://doi.org/10.1111/1744-7917.13435","url":null,"abstract":"Knowing how environmental conditions affect performance traits in pest insects is important to improve pest management strategies. It can be informative for monitoring, but also for control programs where insects are mass-reared, and field-released. Here, we investigated how adult thermal acclimation in sterile Bactrocera dorsalis affects dispersal and recapture rates in the field using a mark-release-recapture method. We also considered how current abiotic factors may affect recapture rates and interact with thermal history. We found that acclimation at 20 or 30 °C for 4 d prior to release reduced the number of recaptures in comparison with the 25 °C control group, but with no differences between groups in the willingness to disperse upon release. However, the deleterious effects of acclimation were only detectable in the first week following release, whereafter only the recent abiotic conditions explained recapture rates. In addition, we found that recent field conditions contributed more than thermal history to explain patterns of recaptures. The two most important variables affecting the number of recaptures were the maximum temperature and the average relative humidity experienced in the 24 h preceding trapping. Our results add to the handful of studies that have considered the effect of thermal acclimation on insect field performance, but notably lend support to the deleterious acclimation hypothesis among the various hypotheses that have been proposed. Finally, this study shows that there are specific abiotic conditions (cold/hot and dry) in which recaptures will be reduced, which may therefore bias estimates of wild population size.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0