Insect Science最新文献_第10页

Ionotropic receptors mediate olfactory learning and memory in Drosophila. 离子受体介导果蝇的嗅觉学习和记忆

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-12-19 DOI: 10.1111/1744-7917.13308

Md Zeeshan Ali, Anushree, Aarif Ahsan, Mohammad Shamsul Ola, Rizwanul Haque, Jawaid Ahsan

{"title":"Ionotropic receptors mediate olfactory learning and memory in Drosophila.","authors":"Md Zeeshan Ali, Anushree, Aarif Ahsan, Mohammad Shamsul Ola, Rizwanul Haque, Jawaid Ahsan","doi":"10.1111/1744-7917.13308","DOIUrl":"10.1111/1744-7917.13308","url":null,"abstract":"Phenylacetaldehyde (PAH), an aromatic compound, is present in a diverse range of fruits including overripe bananas and prickly pear cactus, the two major host fruits for Drosophila melanogaster. PAH acts as a potent ligand for the ionotropic receptor 84a (IR84a) in the adult fruit fly and it is detected by the IR84a/IR8a heterotetrameric complex. Its role in the male courtship behavior through IR84a as an environmental aphrodisiac is of additional importance. In D. melanogaster, two distinct kinds of olfactory receptors, that is, odorant receptors (ORs) and ionotropic receptors (IRs), perceive the odorant stimuli. They display unique structural, molecular, and functional characteristics in addition to having different evolutionary origins. Traditionally, olfactory cues detected by the ORs such as ethyl acetate, 1-butanol, isoamyl acetate, 1-octanol, 4-methylcyclohexanol, etc. classified as aliphatic esters and alcohols have been employed in olfactory classical conditioning using fruit flies. This underlines the participation of OR-activated olfactory pathways in learning and memory formation. Our study elucidates that likewise ethyl acetate (EA) (an OR-responsive odorant), PAH (an IR-responsive aromatic compound) too can form learning and memory when associated with an appetitive gustatory reinforcer. The association of PAH with sucrose (PAH/SUC) led to learning and formation of the long-term memory (LTM). Additionally, the Orco1, Ir84aMI00501, and Ir8a1 mutant flies were used to confirm the exclusive participation of the IR84a/IR8a complex in PAH/SUC olfactory associative conditioning. These results highlight the involvement of IRs via an IR-activated pathway in facilitating robust olfactory behavior.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1249-1269"},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138803954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria. 通过 RNA 干扰靶向 Syntaxin 1A 可抑制蝗虫的摄食和中肠发育。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-29 DOI: 10.1111/1744-7917.13419

Xiaojian Liu, Ya Gao, Yao Li, Jianzhen Zhang

{"title":"Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria.","authors":"Xiaojian Liu, Ya Gao, Yao Li, Jianzhen Zhang","doi":"10.1111/1744-7917.13419","DOIUrl":"https://doi.org/10.1111/1744-7917.13419","url":null,"abstract":"Syntaxin 1A (Syx1A) has diverse and indispensable functions in animals. Previous studies have mainly focused on the roles of Syx1A in Drosophila, and so how Syx1A operates during the development of other insects remains poorly understood. This study investigated whether disrupting LmSyx1A using RNA interference (RNAi) affects the growth and development of Locusta migratoria. LmSyx1A was expressed in all tissues tested, with the highest expression observed in the fat body. After 5th-instar nymphs were injected with double-stranded LmSyx1A (dsLmSyx1A), none of the nymphs were able to molt normally and all eventually died. The silencing of LmSyx1A resulted in the cessation of feeding, body weight loss, and atrophy of the midgut and gastric cecum in locusts. Hematoxylin and eosin (H&E) staining showed that the columnar cells in the midgut were severely damaged, with microvilli defects visible in dsLmSyx1A-injected nymphs. Secretory vesicles were observed with transmission electron microscopy (TEM). In addition, reverse transcription quantitative polymerase chain reaction (RT-qPCR) further indicates that silencing LmSyx1A repressed the expression of genes involved in the insulin/mammalian target of rapamycin (mTOR)-associated nutritional pathway. Taken together, these results suggest that LmSyx1A significantly affects the midgut cell layer of locust nymphs, which was partially associated with the downregulation of the insulin/mTOR-associated nutritional pathway. Thus, we argue that LmSyx1A is a suitable target for developing dsRNA-based biological pesticides for managing L. migratoria.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An olfactory model for evaluating the larviposition preference of a vector fly. 用于评估媒介蝇幼虫定位偏好的嗅觉模型。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-25 DOI: 10.1111/1744-7917.13426

Jing-Hua Chen, Hui Peng, Shuang Wei, Min-Jun Huang, Rui Tang

引用次数: 0

Patterns of morphological evolution in the raptorial appendages of praying mantises. 螳螂猛禽附肢的形态演变模式。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-24 DOI: 10.1111/1744-7917.13423

Alejandro Izquierdo-López, Christine Kiesmüller, Carsten Gröhn, Joachim T Haug, Carolin Haug, Marie K Hörnig

{"title":"Patterns of morphological evolution in the raptorial appendages of praying mantises.","authors":"Alejandro Izquierdo-López, Christine Kiesmüller, Carsten Gröhn, Joachim T Haug, Carolin Haug, Marie K Hörnig","doi":"10.1111/1744-7917.13423","DOIUrl":"https://doi.org/10.1111/1744-7917.13423","url":null,"abstract":"Mantodea (praying mantises) is a group of exclusively predatory insects, which, together with nonraptorial blattodeans (cockroaches and termites) and groups exclusively found in the fossil record, form the group Dictyoptera. A central characteristic of Mantodea is the specialization of their first pair of legs as raptorial grasping appendages, but the evolution from walking to raptorial legs is not yet fully understood. Here, we trace the evolution of the raptorial appendages in Dictyoptera through time using a morphometric (morphospaces) approach. We also describe two new mantodean nymphs preserved in amber from the Cretaceous and Eocene, which expand the scarce mantodean fossil record. Blattodean and mantodean appendages appear distinct in morphospace, but several appendages of fossil non-mantodeans can be considered raptorial, providing a potential transitional link between walking and raptorial morphotypes. Therefore, we discuss potential mantodean affinities for other predatory fossil dictyopterans. We examine changes across extant mantodeans, characterized by a straightening of the tibia especially associated with the rise of the diversification of the Mantidea and discuss whether a thickening of the femur could reflect an early adaptation to cursorial hunting.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNA-mediated insect-resistant transgenic rice poses no risk to a non-target parasitoid, Cotesia chilonis, via direct feeding or through its target host. miRNA 介导的抗虫转基因水稻通过直接取食或目标寄主对非目标寄生虫 Cotesia chilonis 不构成风险。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-22 DOI: 10.1111/1744-7917.13415

Cong Dang, Shan Xiao, Fang Wang, Qi Fang, Hongwei Yao, Kang He, Fei Li, Dawei Xue, Gongyin Ye

{"title":"miRNA-mediated insect-resistant transgenic rice poses no risk to a non-target parasitoid, Cotesia chilonis, via direct feeding or through its target host.","authors":"Cong Dang, Shan Xiao, Fang Wang, Qi Fang, Hongwei Yao, Kang He, Fei Li, Dawei Xue, Gongyin Ye","doi":"10.1111/1744-7917.13415","DOIUrl":"https://doi.org/10.1111/1744-7917.13415","url":null,"abstract":"MicroRNAs (miRNAs) have started to play an important role in pest control, and novel miRNA-based transgenic insect-resistant plants are now emerging. However, an environmental risk assessment of these novel transgenic plants expressing insect miRNAs must be undertaken before promoting their application. Here, transgenic miR-14 rice, which has high resistance to the rice stem borer Chilo suppressalis, was used as an example for evaluation in this study. Taking the tier 1 risk assessment method in Bacillus thuringiensis (Bt) crops as a reference, the effects of the direct exposure of a non-target parasitoid, Cotesia chilonis, to a high concentration of miRNA were evaluated. The results showed that direct feeding with miR-14 at high concentration had no significant effects on the biological parameters of Co. chilonis, whereas when miR-14 was injected into Ch. suppressalis-parasitized larvae, the development duration of Co. chilonis was significantly affected. In combination with the real conditions of the rice paddy field, it could be inferred that transgenic miR-14 rice has no significant negative effects on the important non-target parasitoid, Co. chilonis. These results will provide a foundation for the establishment of a new safety evaluation system for novel RNAi-based transgenic plants.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extended longevity of termite kings and queens is accompanied by extranuclear localization of telomerase in somatic organs and caste-specific expression of its isoforms. 白蚁王和蚁后的长寿与体细胞器官中端粒酶的核外定位及其同工酶种属特异性表达有关。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-21 DOI: 10.1111/1744-7917.13418

Marie Pangrácová, Jan Křivánek, Markéta Vrchotová, Hana Sehadová, Romana Hadravová, Robert Hanus, Ondřej Lukšan

{"title":"Extended longevity of termite kings and queens is accompanied by extranuclear localization of telomerase in somatic organs and caste-specific expression of its isoforms.","authors":"Marie Pangrácová, Jan Křivánek, Markéta Vrchotová, Hana Sehadová, Romana Hadravová, Robert Hanus, Ondřej Lukšan","doi":"10.1111/1744-7917.13418","DOIUrl":"https://doi.org/10.1111/1744-7917.13418","url":null,"abstract":"Kings and queens of termites are endowed with an extraordinary longevity coupled with lifelong fecundity. We recently reported that termite kings and queens display a dramatically increased enzymatic activity and abundance of telomerase in their somatic organs when compared to short-lived workers and soldiers. We hypothesized that this telomerase activation may represent a noncanonical pro-longevity function, independent of its canonical role in telomere maintenance. Here, we explore this avenue and investigate whether the presumed noncanonical role of telomerase may be due to alternative splicing of the catalytic telomerase subunit TERT and whether the subcellular localization of TERT isoforms differs among organs and castes in the termite Prorhinotermes simplex. We empirically confirm the expression of four in silico predicted splice variants (psTERT1-A, psTERT1-B, psTERT2-A, psTERT2-B), defined by N-terminal splicing implicating differential localizations, and C-terminal splicing giving rise to full-length and truncated isoforms. We show that the transcript proportions of the psTERT are caste- and tissue-specific and that the extranuclear full-length isoform TERT1-A is relatively enriched in the soma of neotenic kings and queens compared to their gonads and to the soma of workers. We also show that extranuclear TERT protein quantities are significantly higher in the soma of kings and queens compared to workers, namely due to the cytosolic TERT. Independently, we confirm by microscopy the extranuclear TERT localization in somatic organs. We conclude that the presumed pleiotropic action of telomerase combining the canonical nuclear role in telomere maintenance with extranuclear functions is driven by complex TERT splicing.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of CO₂ elevation on life-history traits of two insecticide-resistant strains of planthopper Nilaparvata lugens on rice. 二氧化碳浓度升高对水稻上两种抗虫性扑粉虱 Nilaparvata lugens 生活史特征的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-21 DOI: 10.1111/1744-7917.13416

Hongran Li, Shanshan Wu, Jing Liu, Yong Chen, Ling Meng, Baoping Li

{"title":"Effects of CO2 elevation on life-history traits of two insecticide-resistant strains of planthopper Nilaparvata lugens on rice.","authors":"Hongran Li, Shanshan Wu, Jing Liu, Yong Chen, Ling Meng, Baoping Li","doi":"10.1111/1744-7917.13416","DOIUrl":"https://doi.org/10.1111/1744-7917.13416","url":null,"abstract":"We made separate experiments to examine life-history traits and activities of protective enzymes as affected by carbon dioxide (CO2) elevation to 780 μL/L as compared to 390 μL/L in imidacloprid- or buprofezin-resistant strains of the brown planthopper (BPH) Nilaparvata lugens. We found an interaction effect between resistance and the CO2 level on the nymphal survival and duration in both resistant strains. Nymphal durations in both resistant strains were much shorter in the resistant than susceptible BPH at 780 μL/L but similar between them or slightly shorter in the resistant than susceptible BPH at 390 μL/L. Nymphal survival was lower for imidacloprid-resistant than its susceptible BPH at 390 μL/L but higher at 780 μL/L; it stayed unaffected by the CO2 elevation in buprofezin-resistant BPH. We did not observe an interaction effect between resistance and the CO2 level on major reproductive parameters in both resistant strains. But the 2 strains were not consistent across CO2 levels in all parameters. Our measurements of protective enzyme activities of superoxide dismutase, catalase, and peroxidase showed an interaction between resistance and the CO2 level. Overall, these enzymes became similar in activity between resistant and susceptible BPH at 780 μL/L compared to 390 μL/L and the change was more distinct in the imidacloprid- than buprofezin-resistant BPH strains. Our findings suggest that CO2 elevation can affect life-history traits of insecticide-resistant BPH, while the effect may vary depending on the kind of insecticides it is resistant to.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Customizable PCR-based target enrichment probes for sequencing fungi-parasitized insects. 基于 PCR 的可定制目标富集探针，用于对真菌寄生的昆虫进行测序。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-21 DOI: 10.1111/1744-7917.13413

Zhengyang Wang, Sangil Kim, Brian D Farrell, Bruno A S de Medeiros

引用次数: 0

Cross-kingdom regulation of silkworm development and cocooning by mulberry microRNAs. 桑树微RNA对家蚕发育和结茧的跨域调控。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-17 DOI: 10.1111/1744-7917.13424

Xinyan Zhou, Jingwei Guo, Chao Qin, Ping Qian, Jiubo Liang, Jinyu Fu, Xinjia Shen, Ningjia He, Chen-Yu Zhang, Xi Chen

引用次数: 0

A novel treatment approach using vitamin B12-conjugated sericin for mitigating nanodiamond-induced toxicity in darkling beetles. 利用维生素 B12 结合的丝胶蛋白减轻纳米金刚石诱发的暗纹甲虫毒性的新型治疗方法。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-07-16 DOI: 10.1111/1744-7917.13421

Marwa Saad, Nabila Selim, Lamia M El-Samad

{"title":"A novel treatment approach using vitamin B12-conjugated sericin for mitigating nanodiamond-induced toxicity in darkling beetles.","authors":"Marwa Saad, Nabila Selim, Lamia M El-Samad","doi":"10.1111/1744-7917.13421","DOIUrl":"https://doi.org/10.1111/1744-7917.13421","url":null,"abstract":"The escalating use of nanodiamonds (NDs) has raised concerns about their ecotoxicological impact, prompting exploration of therapeutic interventions. This paper pioneers the examination of Vitamin B12-conjugated sericin (VB12-SER) as a potential therapeutic approach against ND-induced toxicity in darkling beetles (Blaps polychresta). The study analyzes mortality rates and organ-specific effects, covering the testis, ovary, and midgut, before and after treatments. Following exposure to 10 mg NDs/g body weight, within a subgroup of individuals termed ND2 with a mortality rate below 50%, two therapeutic treatments were administered, including pure sericin (SER) at 10 mg/mL and VB12-SER at 10.12 mg/mL. Consequently, five experimental groups (control, SER, ND2, ND2+SER, ND2+SER+VB12) were considered. Kaplan-Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period. Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs, including cell death, DNA damage, enzyme dysregulation, antioxidant imbalances, protein depletion, lipid peroxidation, and morphological deformities. In contrast, the proposed treatments, especially (ND2+SER+VB12), demonstrated remarkable recovery, highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects. Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins. This research enhances understanding of NDs' effects on B. polychresta, proposing it as an effective bioindicator, and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0