Insect Science最新文献_第10页

Noncanonical action of Relish controls larval development via transcriptional regulation of CncC in Tribolium castaneum. 调味料的非规范作用是通过调控木栗中CncC的转录调控幼体发育。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-31 DOI: 10.1111/1744-7917.70245

Hai Li, Daojie Guan, Huichen Ge, Zhichao Wang, Kun Qian, Hainan Zhang, Jianjun Wang

{"title":"Noncanonical action of Relish controls larval development via transcriptional regulation of CncC in Tribolium castaneum.","authors":"Hai Li, Daojie Guan, Huichen Ge, Zhichao Wang, Kun Qian, Hainan Zhang, Jianjun Wang","doi":"10.1111/1744-7917.70245","DOIUrl":"https://doi.org/10.1111/1744-7917.70245","url":null,"abstract":"The NF-κB transcription factor Relish and Cap \"n\" collar (Cnc) transcription factor CncC primarily regulate innate immune defense and xenobiotic response, respectively, in insects. However, the roles of Relish and CncC in insect development as well as their mutual regulation are still poorly understood. In this study, Relish was cloned from Tribolium castaneum. Spatial-temporal expression analysis revealed that TcRelish expression was highly expressed in the fat body and in 7-d-old female pupae, respectively. Knockdown of TcRelish in 15-d-old larvae resulted in failure of 95% of larvae to pupate, remaining in the larval stage until death. Mechanistically, dsTcRelish treatment led to an increase of juvenile hormone (JH) content by 30.66% concomitant with the upregulation of JH receptor gene Met and JH-responsive gene Kr-h1 by 94.16% and 57.93%, respectively. Notably, knockdown of TcRelish downregulated the expression of JH degradation enzyme genes JHEH2, JHEH3, and JHEH4 by 29.76%, 22.44%, and 23.83%, respectively. Similarly, knockdown of TcCncC resulted in failure of larval pupation, an increase of JH content, upregulation of Met and Kr-h1 as well as downregulation of JHEH2, JHEH3, and JHEH4. Further analysis revealed that the protein level of TcCncC was significantly reduced in 6 d after TcRelish RNAi, whereas stimulation with the Relish agonist NF-κB activator 2 significantly upregulated TcCncC protein levels. Additionally, a luciferase reporter assay revealed that overexpression of TcRelish significantly enhanced the promoter activity of TcCncC by 5.78 fold. These results demonstrate that TcRelish directly regulates larval development through transcriptional regulation of TcCncC, thereby altering the metabolism of JH.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptation of the invasive pest Drosophila suzukii to a specialized nutritional niche. 入侵害虫苏氏果蝇对特殊营养生态位的适应。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-30 DOI: 10.1111/1744-7917.70254

Yan Hou, Ying Zhen

{"title":"Adaptation of the invasive pest Drosophila suzukii to a specialized nutritional niche.","authors":"Yan Hou, Ying Zhen","doi":"10.1111/1744-7917.70254","DOIUrl":"https://doi.org/10.1111/1744-7917.70254","url":null,"abstract":"Investigating the genetic basis of dietary adaptation provides insight into ecological specialization and the evolution of adaptive traits. Nutritional composition of available food sources varies substantially across ecological niches and plays an important role in shaping species distribution and adaptation. Unlike most Drosophila larvae that develop in decaying matters rich in protein and carbohydrate, the larvae of the invasive pest Drosophila suzukii thrive in fresh ripening fruits, a diet characterized by very limited protein and high carbohydrate levels. To understand the intrinsic nutritional adaptation of D. suzukii larvae, we examined its development and fitness on diets spanning a range of protein-to-carbohydrate (P : C) ratios and found that D. suzukii exhibits superior performance on low P : C diets compared to closely related D. biarmipes and D. melanogaster. Transcriptomic analyses showed that D. suzukii underwent fewer transcriptional changes on low P : C diets, with pathways associated with nutrient sensing, transport and digestion contributing to its adaptation. Functional validation in D. melanogaster confirmed that upregulating five genes-dilp3, sNPF, Oct-TyrR, Obp49a, and Dh31-enhanced larval fitness on low P : C diets, supporting their roles in D. suzukii's nutritional adaptation. Together, these results uncover intrinsic molecular mechanisms that enable D. suzukii larvae to successfully colonize a nutritionally challenging niche, and provide potential targets for its pest management strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competing endogenous RNAs (ceRNAs) orchestrate a gene regulatory network in the Aedes aegypti midgut in response to blood feeding. 竞争的内源性rna （ceRNAs）协调了埃及伊蚊中肠对血液摄食的基因调控网络。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-30 DOI: 10.1111/1744-7917.70250

Yujiao Han, Qingshan Ou, Zhuanzhuan Su, Xin Wang, Houming Ren, Xiaolin Yang, Yuqi Lou, Symphony Kashyap, Shiping Liu

{"title":"Competing endogenous RNAs (ceRNAs) orchestrate a gene regulatory network in the Aedes aegypti midgut in response to blood feeding.","authors":"Yujiao Han, Qingshan Ou, Zhuanzhuan Su, Xin Wang, Houming Ren, Xiaolin Yang, Yuqi Lou, Symphony Kashyap, Shiping Liu","doi":"10.1111/1744-7917.70250","DOIUrl":"https://doi.org/10.1111/1744-7917.70250","url":null,"abstract":"Aedes aegypti, a globally widespread mosquito, is a key public health threat due to its ability to transmit multiple deadly arboviruses. The midgut is crucial for the survival, reproduction, and disease transmission of mosquitoes. Competing endogenous RNAs (ceRNAs) regulate gene expression by competing for miRNA response elements (MREs), influencing processes like immunity, growth, and disease. While numerous studies have investigated miRNAs and lncRNAs in mosquitoes, the relationship between ceRNAs and nutrient uptake in the mosquito midgut remains largely unexplored. In this study, we employed whole transcriptome sequencing to identify differentially expressed (DE) miRNAs, mRNAs, lncRNAs, and circRNAs in the midgut of blood-fed Ae. aegypti mosquitoes. We identified 22 DEmiRNAs, 4226 DEmRNAs, 1238 DElncRNAs, and 35 DEcircRNAs, and developed regulatory networks for DE lncRNA/miRNA/mRNA and DE circRNA/miRNA/mRNA interactions. In these networks, DEmRNAs, DEmiRNA targets, and DEcircRNA host genes are enriched in processes like DNA integration, DNA metabolism, membrane lumen sealing, and RNA catalytic activity. KEGG analysis shows these genes are mainly involved in pathways such as mucin-type O-glycan biosynthesis and pantothenic acid and coenzyme A biosynthesis. Notably, miR-305-5p, miR-275-3p, miR-11-5p, miR-33, and miR-34-3p are key components of the ceRNA network, interacting with DElncRNAs, DEcircRNAs, and DEmRNAs. In conclusion, this study identified lncRNAs, mRNAs, miRNAs, and circRNAs in the midgut of Ae. aegypti that respond significantly to blood stimulation, suggesting their key roles in nutrient absorption regulation. These findings enhance our understanding of the molecular mechanisms of nutrient absorption and transformation in Ae. aegypti by clarifying RNA interactions.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivariate analysis reveals the impact of habitat fragmentation and intraspecific competition threshold of urban crickets. 多变量分析揭示了生境破碎化和种内竞争阈值对城市蟋蟀的影响。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-30 DOI: 10.1111/1744-7917.70244

Rong-Yu Xu, Bi-Yun Jia, Shu-Ming Wang, Cai-Xia Wu, Ning-Ning Sun, Xin-Ru Liu, Jia-Huan Yin, Yi-Feng Lin, Pan-Shuai Fei, Bo Du

{"title":"Multivariate analysis reveals the impact of habitat fragmentation and intraspecific competition threshold of urban crickets.","authors":"Rong-Yu Xu, Bi-Yun Jia, Shu-Ming Wang, Cai-Xia Wu, Ning-Ning Sun, Xin-Ru Liu, Jia-Huan Yin, Yi-Feng Lin, Pan-Shuai Fei, Bo Du","doi":"10.1111/1744-7917.70244","DOIUrl":"https://doi.org/10.1111/1744-7917.70244","url":null,"abstract":"Insect survival strategies exhibit significant phenotypic plasticity in response to environmental variation, yet the mechanisms driving sex-specific adaptations under habitat fragmentation remain poorly understood. To address this knowledge gap, we employed a multivariate analysis approach in structural equation modeling (SEM) to analyze long-term data collected from urban habitats. The data includes multiple body size parameters, natural environment parameters, and social environment parameters under distinct levels of intraspecific competition. Body size parameters varied significantly with environmental and social parameters. We then conducted SEM analysis to explore the relationships among four body size parameters, competitive aggression, habitat area and isolation. Our results revealed distinct sex-specific responses: males were negatively selected by natural environment parameters below-threshold and by social environment parameters above-threshold. Females were positively selected by both natural environment parameters and social environment parameters below-threshold, but negatively by social parameters above-threshold. Our findings indicate sex-specific responses to environmental changes. Furthermore, despite the higher intensity of intraspecific competition in above-threshold males compared to below-threshold males, the probability of intraspecific competitive behavior was greater in below-threshold males. We propose the Density-Dependent Competition-Alternative Strategy Trade-Off (DCAST) hypothesis to explain this paradox, wherein males reduce aggression above-threshold as the benefits of alternative strategies outweigh competitive costs. These insect science findings demonstrate how competition thresholds shape phenotypic plasticity in urban insects, providing a framework for understanding adaptive responses to anthropogenic habitat fragmentation.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silkworm as a model for NAD⁺-dependent lifespan regulation: Functional insights into BmNmnat1 and nicotinic acid. 蚕作为NAD +依赖的寿命调节模型：对BmNmnat1和烟酸的功能见解。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-30 DOI: 10.1111/1744-7917.70191

Qingsong Liu, Hongni Li, Hao Chen, Xiaoyang Wang, Guoli Li, Zhangchen Tang, Wei Wang, Yong Hou, Ping Zhao, Qingyou Xia

{"title":"Silkworm as a model for NAD⁺-dependent lifespan regulation: Functional insights into BmNmnat1 and nicotinic acid.","authors":"Qingsong Liu, Hongni Li, Hao Chen, Xiaoyang Wang, Guoli Li, Zhangchen Tang, Wei Wang, Yong Hou, Ping Zhao, Qingyou Xia","doi":"10.1111/1744-7917.70191","DOIUrl":"https://doi.org/10.1111/1744-7917.70191","url":null,"abstract":"Nicotinamide adenine dinucleotide (NAD⁺) is a central metabolic coenzyme that regulates redox homeostasis, DNA repair, and cellular longevity. While the role of NAD⁺ metabolism in mammalian aging has been well studied, its significance in invertebrate systems remains underexplored. Here, we establish the silkworm (Bombyx mori) as a novel model for investigating NAD⁺-dependent lifespan regulation. Through phenotypic comparisons among silkworm strains, we found that longer-lived strains exhibit higher levels of NAD⁺ and elevated expression of BmNmnat1, a key enzyme in NAD⁺ biosynthesis. CRISPR/Cas9-mediated knockout and RNAi knockdown of BmNmnat1 led to embryonic lethality, increased DNA damage, disrupted cell cycle progression, and morphological aging phenotypes. Supplementation with nicotinic acid (NA) significantly reversed these aging-associated changes both in vitro and in vivo, including improved redox balance, reduced oxidative stress markers, and prolonged adult lifespan. Our results highlight the evolutionarily conserved role of BmNmnat1-mediated NAD⁺ metabolism in aging and establish the silkworm as a valuable invertebrate model for mechanistic studies and antiaging intervention screening.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fkh dual regulation of promoter activation and miR-34-p38b axis enhances IMD pathway immune response in Drosophila. Fkh双调控启动子激活和miR-34-p38b轴增强果蝇IMD通路免疫应答。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-30 DOI: 10.1111/1744-7917.70251

Nan Zhang, Ju Li, Yongkang Yuan, Fengjie Li, Ping Jin, Fei Ma

{"title":"Fkh dual regulation of promoter activation and miR-34-p38b axis enhances IMD pathway immune response in Drosophila.","authors":"Nan Zhang, Ju Li, Yongkang Yuan, Fengjie Li, Ping Jin, Fei Ma","doi":"10.1111/1744-7917.70251","DOIUrl":"https://doi.org/10.1111/1744-7917.70251","url":null,"abstract":"Innate antibacterial defense in Drosophila relies on the IMD pathway to induce antimicrobial peptides (AMPs), but the auxiliary transcriptional networks that amplify or fine-tune this response remain poorly defined. Here, we identify the Fork head (Fkh) transcription factor, a FoxA-family protein classically linked to development and metabolism, as a critical amplifier of humoral immunity. Our results show that bacterial infection rapidly induces Fkh expression, and enhances host survival, as well as promotes robust induction of AMPs including Diptericin (Dpt), Attacin-A, and Cecropin-A1. Mechanistically, Fkh directly binds the Dpt promoter and synergizes with the NF-κB factor Relish, ensuring strong promoter activation. In parallel, Fkh activates miR-34 transcription to repress the negative regulator p38b, thereby relieving inhibitory pressure on IMD signaling. Disruption of either Fkh-binding motifs or the miR-34 seed site abolishes these effects. Together, our findings uncover a dual regulatory strategy in which Fkh simultaneously acts as a promoter-bound transcription factor and as an upstream activator of an immune-enhancing miRNA. This integrated Fkh-miR-34-p38b axis establishes a feed-forward mechanism that ensures rapid and high-amplitude antibacterial defense, providing a new paradigm for transcription factor-miRNA cooperation in innate immunity.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut bacterial-mediated adaptation of Streltzoviella insularis to a constitutive defense compound in an ash tree (Fraxinus sp.). 肠细菌介导的岛链菌对白蜡树（白蜡树属）组成防御化合物的适应。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-28 DOI: 10.1111/1744-7917.70217

Qingjie Cao, Can Wang, Tuuli-Marjaana Koski, Yingqi Zhang, Zenghe Bu, Limin Zhang, Jianghua Sun

{"title":"Gut bacterial-mediated adaptation of Streltzoviella insularis to a constitutive defense compound in an ash tree (Fraxinus sp.).","authors":"Qingjie Cao, Can Wang, Tuuli-Marjaana Koski, Yingqi Zhang, Zenghe Bu, Limin Zhang, Jianghua Sun","doi":"10.1111/1744-7917.70217","DOIUrl":"10.1111/1744-7917.70217","url":null,"abstract":"Gut microbiota play a key role in an insect's ability to overcome host tree defenses. Streltzoviella insularis (Lepidoptera: Cossidae) is a generalist wood-boring pest in China, and Fraxinus chinensis var. rhynchophylla is its preferred host in Xiong'an New Area. Previous mechanistic studies have demonstrated that S. insularis can tolerate and degrade host defense compounds via its gut microbes. However, whether hydroxytyrosol (a constitutive phenolic compound potentially involved in browning reactions) functions as a key defense of Fraxinus chinensis var. rhynchophylla against woodborers, and whether the gut microbiota of attacking S. insularis can degrade hydroxytyrosol, remains unclear. We determined the hydroxytyrosol content in S. insularis-infested and uninfested Fraxinus chinensis var. rhynchophylla. We further evaluated the effects of hydroxytyrosol on larval survival, tested the ability of larval gut bacteria to degrade hydroxytyrosol, and identified the resulting degradation products. Although hydroxytyrosol was toxic to S. insularis, its concentrations were lower in larval galleries than in uninfested trees, suggesting that S. insularis can degrade this compound. Gut bacteria from S. insularis degraded hydroxytyrosol both in vivo and in vitro, and Serratia sp. showed the strongest hydroxytyrosol degradation activity. Metabolomics further indicated that gut bacteria may degrade hydroxytyrosol via the tricarboxylic acid cycle pathway. Because this pathway yields ATP, the bacterial degradation products of hydroxytyrosol may provide an energy source for the insect hosts or their gut microorganisms.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146062516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From settled to dispersal: density-dependent behavioral switching in the pea aphid, Acyrthosiphon pisum. 从定居到分散：豌豆蚜虫的密度依赖行为转换。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-21 DOI: 10.1111/1744-7917.70221

Xing-Xing Wang, Ya-Nan Liu, Jun-Chao Xu, Yun Yang, Tong-Xian Liu, Yi Zhang

{"title":"From settled to dispersal: density-dependent behavioral switching in the pea aphid, Acyrthosiphon pisum.","authors":"Xing-Xing Wang, Ya-Nan Liu, Jun-Chao Xu, Yun Yang, Tong-Xian Liu, Yi Zhang","doi":"10.1111/1744-7917.70221","DOIUrl":"https://doi.org/10.1111/1744-7917.70221","url":null,"abstract":"Group-living insects like the pea aphid, Acythosiphon pisum, use population density as a key signal to regulate behavior, but the underlying mechanisms of individual responses remain unclear. This study employed a novel dual-color system to precisely track individual aphid behavior across a density gradient. We found that aphid behavioral responses are not linear but are triggered at critical density thresholds, which differ depending on the aphid's initial state (settled or active). As density increased, aphids exhibited significantly greater movement and reduced reproductive output, with a key behavioral shift toward dispersal occurring above 1.25 individuals/cm2 (1: 11 group). To investigate the molecular basis for this shift, we analyzed the expression of genes related to three key monoamine neurotransmitters. The results showed a strong positive correlation between population density, locomotor activity, and the expression of the tyramine β-hydroxylase (TBH) gene, which is critical for octopamine synthesis. Conversely, the expression of the serotonin-related gene tryptophan hydroxylase (TPH) decreased, while the dopamine-related gene Tyrosine hydroxylase (TH) showed no clear trend. These findings suggest that aphids utilize a \"threshold-triggered\" model to rapidly shift from a settled to a dispersal state, a process primarily mediated by the monoamine neurotransmitters system. This provides a new perspective on the self-regulation mechanisms of insect populations.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ant-aphid mutualism: the influence of Tapinoma ibericum (Hymenoptera: Formicidae) on Aphis gossypii (Hemiptera: Aphidae) control by commercial and spontaneous natural enemies. 寄生蜂对商业天敌和自发天敌对棉蚜控制的影响。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-21 DOI: 10.1111/1744-7917.70249

Jesús Foronda, Estefanía Rodríguez, Mónica González, María Del Mar Téllez, Carmen Robles-Vallet, Pablo Barranco, Felipe Pascual, Francisca Ruano

{"title":"Ant-aphid mutualism: the influence of Tapinoma ibericum (Hymenoptera: Formicidae) on Aphis gossypii (Hemiptera: Aphidae) control by commercial and spontaneous natural enemies.","authors":"Jesús Foronda, Estefanía Rodríguez, Mónica González, María Del Mar Téllez, Carmen Robles-Vallet, Pablo Barranco, Felipe Pascual, Francisca Ruano","doi":"10.1111/1744-7917.70249","DOIUrl":"https://doi.org/10.1111/1744-7917.70249","url":null,"abstract":"The cotton aphid (Aphis gossypii) is a major pest of greenhouse peppers in southern Spain. Biological control using the parasitoid wasp Aphidius colemani is limited because the ant Tapinoma ibericum, engaged in a mutualistic relationship with aphids, protects them from parasitoid attack and thereby reduces parasitism success. To assess the impact of ants on pest biological control, an ant-exclusion experiment was conducted over two consecutive years in four experimental greenhouses. Pepper plants were infested with aphids and then treated with Aphidius colemani. The presence of natural enemies and fruit production were also evaluated. Excluding ants significantly increased the abundance of mummies by 22.2% and reduced the number of aphid colonies, while total aphid abundance showed variable, year-dependent effects and was not affected by ants. Ant presence negatively affected some natural enemies such as Chrysoperla carnea s.l., the mirid Nesidiocoris tenuis, and the ladybird Scymnus sp., had no effect on hoverflies and spiders, and increased the abundance of Aphidoletes aphidimyza, indicating contrasting responses among natural enemies. Fruit weight was not affected by ant presence. Overall, these findings confirm that T. ibericum reduces parasitism by A. colemani, and demonstrate that it modifies aphid spatial distribution, and reshapes the natural enemy community, but does not necessarily diminish crop production. These results suggest that combining A. aphidimyza with A. colemani could improve control of A. gossypii in ant-infested crops.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmATAD3A negatively regulates BmIGF2BP1 to promote BmNPV proliferation. BmATAD3A负调控BmIGF2BP1促进BmNPV增殖。

IF 3 1区农林科学

Insect Science Pub Date : 2026-01-19 DOI: 10.1111/1744-7917.70237

Yu Lin, Jie Wang, Yan Luo, Feifei Liu, Peng Chen, Cheng Lu, Zhanqi Dong, Minhui Pan

{"title":"BmATAD3A negatively regulates BmIGF2BP1 to promote BmNPV proliferation.","authors":"Yu Lin, Jie Wang, Yan Luo, Feifei Liu, Peng Chen, Cheng Lu, Zhanqi Dong, Minhui Pan","doi":"10.1111/1744-7917.70237","DOIUrl":"https://doi.org/10.1111/1744-7917.70237","url":null,"abstract":"Baculoviruses, large double-stranded DNA viruses, are widely used in biological control and biotechnology; however, their success depends on complex interactions with host factors. Members of the AAA ATPase family are emerging as key regulators in these processes. Previous studies identified Bombyx mori ATAD3A (BmATAD3A) as a host factor hijacked by the viral protein LEF-11 to facilitate Bombyx mori nucleopolyhedrovirus (BmNPV) replication. However, the underlying mechanisms remain poorly understood. We generated transgenic silkworms overexpressing BmATAD3A and demonstrated that its overexpression enhances viral proliferation in vivo. Co-immunoprecipitation (Co-IP) combined with mass spectrometry analysis identified BmIGF2BP1 as an interacting protein of BmATAD3A. Further analysis showed that BmATAD3A negatively regulated the expression of BmIGF2BP1. Additionally, our results showed that BmIGF2BP1 suppresses BmNPV replication, while BmATAD3A-mediated downregulation of BmIGF2BP1 further promotes viral proliferation. This study solidifies the critical role of ATAD3A in BmNPV replication, identifies its interacting proteins, and elucidates the functional interplay between BmIGF2BP1 and BmATAD3A in viral propagation. These findings advance our understanding of the mechanisms by which AAA ATPase family members regulate viral replication, thereby enriching the baculovirus-host interaction network.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0