Insect Science最新文献

Dissecting the roles of Taiman in pupation and female reproduction of Helicoverpa armigera. 台湾人在棉铃虫化蛹和雌性繁殖中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-30 DOI: 10.1111/1744-7917.70096

Long Ma, Hao-Jie Zheng, Hong-Bin Ge, Ying-Chuan Peng, You-Xin Yang, Zi-Wei Zhao, Wan-Na Zhang, Hai-Jun Xiao

{"title":"Dissecting the roles of Taiman in pupation and female reproduction of Helicoverpa armigera.","authors":"Long Ma, Hao-Jie Zheng, Hong-Bin Ge, Ying-Chuan Peng, You-Xin Yang, Zi-Wei Zhao, Wan-Na Zhang, Hai-Jun Xiao","doi":"10.1111/1744-7917.70096","DOIUrl":"https://doi.org/10.1111/1744-7917.70096","url":null,"abstract":"In insects, metamorphosis and reproduction are coordinated by juvenile hormone (JH) and 20-hydroxyecdysone (20E). A transcription factor Taiman (Tai) serves as a coactivator of the functional 20E receptor and heterodimerizes with JH receptor Methoprene-tolerant (Met) to form an active receptor complex, encoding a key modulator of JH and 20E signaling pathways. However, direct evidence for the participation of Tai in metamorphic actions and vitellogenesis is quite limited. Herein, 1 isoform of Tai was identified from Helicoverpa armigera, named HaTai; the transcription of HaTai was stimulated by either JH or 20E ingestion. Knocking down HaTai in 5th instar larvae caused severe pupation failure and weight loss, wherein 69.44% of HaTai-RNAi (RNA interference) larvae died from pupation failure within 6 d. The HaTai-RNAi larvae exhibited attenuated JH signaling but intensified ecdysteroidogenesis, whereas supplementation with JH or 20E failed to recover the expression of HaTai. In female adults, depleting HaTai blocked vitellogenin transcription and caused atrophied ovaries with deficient yolk protein deposition. The HaTai-depleted females exhibited a substantial decrease in egg production. Moreover, the HaTai-depleted fat body became remarkably loose and partly vacuolated, accompanied by a reduction of triglyceride content. To explore the feasibility of oral-ingested RNAi, feeding chitosan-wrapped double-stranded RNA targeting Tai induced a pronounced silencing effect in larvae, causing a significant increase in larval mortality and a decrease in pupation rate. Our study therefore unveiled the complex roles of HaTai in pupation and JH-mediated vitellogenesis, and explored an effective RNAi method applicable in H. armigera.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The JAK-STAT pathway in invertebrates: An emerging battleground for host‒virus warfare. 无脊椎动物的JAK-STAT通路：宿主-病毒战争的新战场

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-30 DOI: 10.1111/1744-7917.70109

Hengchuan Xia, Cong Zhang, Zhongjian Guo, Liang Chen, Keping Chen

{"title":"The JAK-STAT pathway in invertebrates: An emerging battleground for host‒virus warfare.","authors":"Hengchuan Xia, Cong Zhang, Zhongjian Guo, Liang Chen, Keping Chen","doi":"10.1111/1744-7917.70109","DOIUrl":"https://doi.org/10.1111/1744-7917.70109","url":null,"abstract":"The arms race between hosts and viruses for dominance over the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway has been extensively studied in vertebrates but remains poorly explored in invertebrates. Recent studies have revealed that the antiviral Vago/JAK-STAT pathway in invertebrates operates as a parallel mechanism to the vertebrate interferon (IFN)/JAK-STAT pathway, suggesting a highly conserved antiviral mechanism across the animal kingdom. Moreover, invertebrates appear to employ multifaceted strategies to modulate the JAK-STAT pathway to inhibit viral replication or enhance host tolerance, while viruses can counteract by manipulating the JAK-STAT pathway to their advantage. Thus, the competition between hosts and viruses for control of the JAK-STAT pathway also exists in invertebrates, indicating this arms race is also conserved in evolution. This review summarizes the discovery of the Vago/JAK-STAT pathway, its comparison to the IFN/JAK-STAT pathway, and the mechanisms underlying the host-virus tug-of-war over the JAK-STAT pathway in invertebrates. These insights shed light on this highly conserved immune strategy, its implication for host-virus interaction, and its potential application in disease control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of pig manure-derived gut microbiota in accelerating the growth and development of Sarcophaga africa. 猪粪便来源的肠道微生物群在加速非洲石棺菌生长和发育中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-30 DOI: 10.1111/1744-7917.70104

Fengqin Yang, Xiangyan Zhang, Yanjie Shang, Jiao Xiao, Jian Zhao, Xingchun Zhao, Yadong Guo

{"title":"The role of pig manure-derived gut microbiota in accelerating the growth and development of Sarcophaga africa.","authors":"Fengqin Yang, Xiangyan Zhang, Yanjie Shang, Jiao Xiao, Jian Zhao, Xingchun Zhao, Yadong Guo","doi":"10.1111/1744-7917.70104","DOIUrl":"https://doi.org/10.1111/1744-7917.70104","url":null,"abstract":"Exploring the impact of diet-derived gut microbiota on insect growth and development holds significant value not only for understanding the \"diet-gut microbes-insects\" interactions, but also for forensic entomology, as it lays the research foundation for clarifying the relationships among \"cadavers-microbes-necrophagous insects\". This study investigates the effects of pig manure-derived gut microbiota on the growth and development of Sarcophaga africa, a key necrophagous insect. Our findings demonstrate that, compared to pig lung, pig manure significantly increased larval amount without affecting adult eclosion rates, thereby facilitating laboratory population establishment and accelerating development. Through microbial sequencing, key target bacteria were identified and validated using quantitative detection and antibiotic interference experiments. The results highlight two critical aspects: first, dietary microbes play a central role in shaping the gut microbial community structure of larvae; second, gut microbes derived from pig manure, particularly Clostridium_sensu_stricto_1, may enhance the developmental process of S. africa by directly increasing protein levels in 3rd instar larvae and indirectly reducing glycogen levels in late pupae. Additionally, developmental data for S. africa at 30 °C were obtained to establish reference values for forensic applications using necrophagous insects in postmortem interval estimation. These findings highlight the need for forensic entomologists to consider how microbial-driven developmental variations may affect the reliability of developmental data in such studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mating affects dopamine signaling in Drosophila melanogaster females. 交配影响雌性黑腹果蝇的多巴胺信号。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-29 DOI: 10.1111/1744-7917.70112

Elena V Burdina, Margarita A Bobrovskikh, Natalya V Adonyeva, Alexander A Alekseev, Olga V Andreenkova, Maksim A Deryuzhenko, Olga D Shishkina, Evgenia K Karpova, Nataly E Gruntenko

引用次数: 0

The role of dopaminergic neuronal clusters in governing division of labor in ants. 多巴胺能神经元簇在蚂蚁劳动分工中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-27 DOI: 10.1111/1744-7917.70087

Wenjiang Zhong, Nianxia Xie, Guo Ding, Jie Zhao, Pei Zhang, Qiye Li, Hao Ran, Guojie Zhang, Weiwei Liu

{"title":"The role of dopaminergic neuronal clusters in governing division of labor in ants.","authors":"Wenjiang Zhong, Nianxia Xie, Guo Ding, Jie Zhao, Pei Zhang, Qiye Li, Hao Ran, Guojie Zhang, Weiwei Liu","doi":"10.1111/1744-7917.70087","DOIUrl":"https://doi.org/10.1111/1744-7917.70087","url":null,"abstract":"Reproductive division of labor is one of the most prominent features of social insects. Yet, the neural mechanisms that govern this division and the associated behavioral differentiation among castes remain obscure. In this study, we systematically characterized the anatomical features of dopamine neurons in the brain of Monomorium pharaonis and compared the cell number and spatial distribution of these neurons across castes. We identified 17 anatomically distinct clusters of dopamine neurons in the ant brain, with cell numbers varying from ∼322 to ∼431 across castes. The major dopamine clusters are located in brain regions analogous to those in flies, with 2 clusters, PAL and PPL1 exhibiting significantly higher cell numbers in ants than in flies. Notably, 4 clusters, DAM, D1, DPL, and PPL2, showed remarkable variation in cell numbers across castes. Using single-cell transcriptomics, we identified specific molecular markers for subdividing dopamine neurons. We validated the expression of multiple neuropeptide genes in specific dopamine clusters. In particular, we found that PPL2 cluster can be further divided into 2 subclusters, PPL2a and PPL2b, which are partially labeled by the peptide gene Nplp1. PPL2b neurons, characterized by larger cell bodies, and present only in unmated queens and males, are absent in mature queens and workers. These neurons are located adjacent to Nlg2-expressing lobular neurons, which are also absent in workers and may play a role in regulating mating behaviors. Our findings provide a foundation for further investigation into the neural mechanism underlying division of labor and caste-specific behaviors in ant.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bi-environmental cage for colony management in the mass rearing of Anastrepha ludens (Diptera: Tephritidae). 双环境网箱在大规模饲养小绒蚤中的群体管理（双翅目：绒蚤科）。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-27 DOI: 10.1111/1744-7917.70105

José S Meza, Jorge Ibañez-Palacios, Daisy P Cardenas-Enriquez, Juan H Luis-Alvares, Pablo Liedo

{"title":"Bi-environmental cage for colony management in the mass rearing of Anastrepha ludens (Diptera: Tephritidae).","authors":"José S Meza, Jorge Ibañez-Palacios, Daisy P Cardenas-Enriquez, Juan H Luis-Alvares, Pablo Liedo","doi":"10.1111/1744-7917.70105","DOIUrl":"https://doi.org/10.1111/1744-7917.70105","url":null,"abstract":"Anastrepha ludens (Loew) is controlled in Mexico using sterile insect technique (SIT). SIT relies primarily on mass-reared insects, which are subjected to the effects of selection during colonization and rearing, and that frequently result in modifications of their biology and behavior. Here, we propose and evaluate a novel \"bi-environmental cage\" for colony management which promotes more natural sexual selection. The cage allows each sex to reach sexual maturity in separate compartments acclimatized according to natural conditions. Females mature in areas where they can recognize oviposition sites, while males mature in areas populated with small trees to allow establishment of territories in leks and performance of courtship behaviors. To determine whether the bi-environmental cage can minimize the potential adverse effects on mating competitiveness, two strains of A. ludens were tested; wild flies strain and genetic sexing strain Tapachula 7 (mass-reared flies). We found that after 4 generations in the mass-reared flies in the bi-environmental cage showed a level of fecundity similar to that of flies from the conventional cage. A similar pattern was also seen in the case of wild flies in both types of cages. In addition, other biological attributes of the wild strain assessed over six generations showed adaptability to mass-rearing conditions. Wild males from the bi-environmental cages were more sexually competitive than those from the conventional cage. Our results show that it is possible to mitigate many of the detrimental effects of domestication on the sexual performance of mass-reared males by using close-to-natural conditions for colony management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic bases and robustness of the toxicological response to spinosad and lambda-cyhalothrin in Drosophila melanogaster. 黑胃果蝇对spinosad和lambda- cyhalthrin毒理学反应的遗传基础和稳健性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-27 DOI: 10.1111/1744-7917.70107

Mariana C Sabio, Raúl Adolfo Alzogaray, Madelein S M Ortiz, Juan José Fanara

{"title":"Genetic bases and robustness of the toxicological response to spinosad and lambda-cyhalothrin in Drosophila melanogaster.","authors":"Mariana C Sabio, Raúl Adolfo Alzogaray, Madelein S M Ortiz, Juan José Fanara","doi":"10.1111/1744-7917.70107","DOIUrl":"https://doi.org/10.1111/1744-7917.70107","url":null,"abstract":"Insecticides are valuable and widely used tools for the control of insect pests. However, we know very little about the genes and processes involved in the key steps of the poisoning sequence. Besides, the excessive and indiscriminate use of insecticides is generating mechanisms of resistance in various insect pests. To improve our understanding of insecticide toxicity, we need a powerful model organism to help describe the processes underlying insecticide poisoning and the use of genomic tools to identify and analyze the genetic basis of the toxicological response of the insecticides. In this context, we associate variation in toxicological response with genomic variation, to identify genetic polymorphisms underlying the different steps of the insect (genotype)-response (phenotype)-insecticide (environment) interaction. We investigate the genetic factors involved in the toxicological response of D. melanogaster lines when exposed to spinosad and lambda-cyhalothrin through the measured of KT50 in adult males. Since the genomes of all lines evaluated are completely sequenced, we performed a Genome Wide Association Study that enabled us to identify genetic polymorphisms and candidate genes responsible for the overall phenotypic variation. Most of the candidate genes detected exhibited insecticide-specific effect and play roles in the toxicodynamics as AstC-R1, Dh44-R, stan, Ca-β, AgmNAT, Acox57D-d, Btk, CarT, dpr8, Pkd2, and Shab and the toxicokinetics like for example ckd, Hr38, robo2, Toll-4, Eglp2, and Prip of spinosad and lambda-cyhalothrin. Finally our results suggest that in the case of lambda-cyhalothrin genotypes exhibiting resistance phenotypes (higher KT50) also displaying a less constant (robust) response.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dilemma of honey bee pest management in European Union: eradication or coexistence? 欧盟蜜蜂病虫害管理的困境：根除还是共存？

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70097

Michela Bertola, Franco Mutinelli

引用次数: 0

Mesoporous silica nanoparticles enhance the toxicity of chlorantraniliprole to Spodoptera frugiperda by possibly inhibiting energy metabolism and chitin protein synthesis. 介孔二氧化硅纳米颗粒可能通过抑制能量代谢和几丁质蛋白合成来增强氯虫腈对夜蛾的毒性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70106

Suman Zong, Dejin Xu, Yiping Jiang, Xiaofeng Wang, Keyan Zhu-Salzman, Xin Zhang, Jing Zhao, Liubin Xiao, Leigang Zhang, Guangchun Xu, Aiguo Gu, Hao Hong, Linquan Ge, Yongan Tan

{"title":"Mesoporous silica nanoparticles enhance the toxicity of chlorantraniliprole to Spodoptera frugiperda by possibly inhibiting energy metabolism and chitin protein synthesis.","authors":"Suman Zong, Dejin Xu, Yiping Jiang, Xiaofeng Wang, Keyan Zhu-Salzman, Xin Zhang, Jing Zhao, Liubin Xiao, Leigang Zhang, Guangchun Xu, Aiguo Gu, Hao Hong, Linquan Ge, Yongan Tan","doi":"10.1111/1744-7917.70106","DOIUrl":"https://doi.org/10.1111/1744-7917.70106","url":null,"abstract":"Nanosized formulations are an important means to develop effective pesticide molecules against target pests with improved environmental safety. In this study, we constructed a nanodelivery system using mesoporous silica nanoparticles (MSNs) carrying chlorantraniliprole (CLAP). The drug loading rate was determined using liquid chromatography, and CLAP@MSNs were characterized and analyzed. Adhesion was measured by contact angle and surface tension, and UV resistance was assessed. The transport of CLAP@MSNs within plants was observed using confocal fluorescence microscopy. Additionally, larval bioassay experiments and RNA-seq analyses were conducted on CLAP@MSNs. The results indicate that CLAP (35.6% w/w) has been successfully loaded onto MSNs. CLAP@MSNs appeared to have structure and size similar to MSNs. CLAP@MSNs showed effective adhesion to the surface of corn leaves and stems and also resisted to UV photolysis. Bidirectional delivery of fluorescently labeled CLAP@MSNs through vascular bundles in corn. When administered to Spodoptera frugiperda, mortality in CLAP@MSNs-treated larvae increased whereas weight and developmental period decreased significantly compared with larvae treated with CLAP alone. RNA-seq analysis revealed that oral administration of CLAP@MSNs led to the downregulation of genes associated with drug metabolism, energy metabolism, and chitin protein synthesis, thereby inhibiting the growth and development of insects. Interestingly, CLAP@MSNs exerted no harmful effects on growth of plants and development of non-target organisms. Taken together, CLAP@MSNs provide a safe, effective, and economical insecticidal nanopesticide system that potentially further improves the effectiveness of CLAP against lepidopteran pests, contributing to the reduction of pesticide use in pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-701 modulates the humoral immune response of Coptotermes formosanus against Metarhizium anisopliae. miR-701调节台湾白蚁对金龟子绿僵菌的体液免疫反应

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70095

Weiwen Chen, Zhiqiang Li

{"title":"miR-701 modulates the humoral immune response of Coptotermes formosanus against Metarhizium anisopliae.","authors":"Weiwen Chen, Zhiqiang Li","doi":"10.1111/1744-7917.70095","DOIUrl":"https://doi.org/10.1111/1744-7917.70095","url":null,"abstract":"Termites are not only social insects but also significant global insect pests. Investigating the molecular mechanisms regulating immune defense response in termites is beneficial for developing novel approaches to termite management. Currently, research mainly focuses on coding RNAs in termite immunity, with limited exploration of non-coding RNAs. Here, we identified miR-701, a markedly downregulated microRNA (miRNA) in the globally significant termite pest Coptotermes formosanus after Metarhizium anisopliae infection, which targets the immune gene Toll4. Transcriptome analysis of termites injected with miR-701 agomir revealed that miR-701 affects the immune-related response, growth, and development of termites. Treatment with miR-701 agomir, either through injection or ingestion, resulted in a notably reduced survival rate of termites infected with M. anisopliae compared to the control group infected with M. anisopliae alone. Additionally, termites injected with miR-701 agomir exhibited a significant decrease in the expression of antimicrobial peptide genes termicin and lysozyme, alongside a notable increase in the colony-forming units of M. anisopliae in the infected termites. Subsequent investigations demonstrated that miR-701 suppressed the expression of the target gene Toll4, consequently inhibiting the Toll signaling pathway and diminishing the expression of antimicrobial peptides. These findings suggest that termites can combat M. anisopliae by downregulating miR-701 expression to activate the Toll signaling pathway and enhance antimicrobial peptides synthesis. This discovery improves our comprehension of the role of miRNAs in termites' immune responses and the mechanism of termites managing miRNAs to boost their pathogen resistance. Additionally, it reveals a new molecular target for termite biological control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0