Insect Science最新文献

{"title":"Identification and functional analysis of CYP9A32, a cytochrome P450 gene associated with emamectin benzoate resistance in Spodoptera frugiperda.","authors":"Jia-Yu Lin, Shi-Wen Deng, Wei Liang, Muhammad Shoaib Rana, Mutaz Mohammed Abdallah, Jia-Xin Li, Rui-Long Wang","doi":"10.1111/1744-7917.70179","DOIUrl":"https://doi.org/10.1111/1744-7917.70179","url":null,"abstract":"<p><p>The fall armyworm (Spodoptera frugiperda, J. E. Smith) is a highly invasive pest causing significant damage to global crops and has developed resistance to multiple insecticides. Cytochrome P450 monooxygenases (P450s) are crucial for insecticide detoxification and resistance. However, the roles of P450 genes in mediating resistance to emamectin benzoate in S. frugiperda are unclear. This study examined the expression of 15 P450 genes in susceptible and emamectin benzoate-resistant populations. CYP9A32 was highly expressed in resistant populations (EBRS, FZJRS, and FHKRS) with resistance ratios of 32.7-, 28.7-, and 48.9-fold, respectively. P450 activity was significantly higher in these resistant populations than in the susceptible population (SS), with increases of 3.52-, 7.23-, and 6.12-fold. In contrast, no significant differences were observed in glutathione S-transferase and esterase activities. RNA interference (RNAi) of CYP9A32 increased susceptibility to emamectin benzoate and reduced P450 activity by 38.74%, 26.38%, and 21.03% in SS, EBRS, and FZJRS populations, respectively. Mortality rates of 3rd instar larvae treated with emamectin benzoate at median lethal concentration dosage increased significantly due to CYP9A32 RNAi, with increases of 92.98%, 64.06%, 51.61%, and 65.52% for SS, EBRS, FZJRS, and FHKRS populations. Conversely, transgenic Drosophila melanogaster overexpressing CYP9A32 showed enhanced tolerance to emamectin benzoate. Homology modeling and molecular docking analyses revealed tight binding of CYP9A32 to emamectin benzoate. These findings demonstrate that CYP9A32 upregulation contributes to emamectin benzoate resistance in S. frugiperda. This study enhances our understanding of P450-mediated resistance mechanisms and provides insights for improving insecticide resistance management in pest control programs.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DsiRNA-mediated silencing of Ceratitis capitata transformer or transformer-2 leads to masculinization of XX embryos and systemic gene silencing in ovaries.","authors":"Gennaro Volpe, Sarah Maria Mazzucchiello, Domenico De Falco, David Torrente, Stefania Liguori, Noemi Rosati, Dora Baccaro, Michela Mazzeo, Fulvio Bertolotto, Harshini Sangle, Ennio Giordano, Angela Carfora, Francesca Lucibelli, Marianna Varone, Paola Di Lillo, Hugo Diego Perdomo, Mariangela Bonizzoni, Serena Aceto, Marco Salvemini, Molly Duman-Scheel, Giuseppe Saccone","doi":"10.1111/1744-7917.70173","DOIUrl":"https://doi.org/10.1111/1744-7917.70173","url":null,"abstract":"<p><p>Ceratitis capitata (medfly), a major agricultural pest, is predominantly controlled using chemical insecticides, which pose environmental risks. Ecosustainable alternatives, such as the Sterile Insect Technique (SIT), rely on the mass release of sterile male-only progeny. However, sexing of male offspring requires the elimination of females during development. To overcome the loss of 50% of the progeny, the sex reversal of females into XX fertile males at embryonic stages by dsRNA injections was effective but not scalable. This study demonstrates the efficacy of Dicer-substrate small interfering RNAs (DsiRNAs) as an alternative to long double-stranded RNAs (dsRNAs) for targeting the sex determination genes Cctransformer and Cctransformer-2 in the medfly, which causes full masculinization of XX individuals. Injection of DsiRNAs into XX embryos induced the expression of male-specific Cctra isoforms within a few hours, resulting in the development of adult masculinized XX flies. Additionally, thoracic injection of DsiRNAs in adult females achieved systemic gene silencing, reducing Cctra/Cctra-2 transcript levels in the ovaries by 75%-80% within 48 h. The reduced size of DsiRNAs compared to dsRNAs enhances their potential for alternative delivery methods, including embryo permeabilization, electroporation, and feeding in larvae or adult females. These findings provide a potential foundation for future scalable conditional masculinization of XX individuals rather than relying on female lethality, doubling male-only productivity. Developing novel sexing methods as an alternative to transgenic approaches will expand the applicability of SIT.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of PxCBR1-L reveals its essential role in multi-stress tolerance in a cosmopolitan pest.","authors":"Xin Miao, Yu Zhang, Bingwei Chen, Zongyao Ma, Haiyin Su, Bingxue Li, Yuanyuan Liu, Gaoke Lei, Shijun You","doi":"10.1111/1744-7917.70164","DOIUrl":"https://doi.org/10.1111/1744-7917.70164","url":null,"abstract":"<p><p>Climate change and intensive pesticide use impose combined stress on agricultural pests. To investigate the genetic basis of stress resilience in Plutella xylostella, we focused on PxCBR1-L, a carbonyl reductase gene highly expressed in detoxification tissues and upregulated under heat and insecticide exposure. Using clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9, we generated PxCBR1-L knockout lines. Mutants exhibited impaired development, reduced survival, shortened lifespan, and decreased fecundity. Biochemically, knockout strains showed elevated reactive oxygen species and reduced superoxide dismutase, catalase, Carboxylesterase, glutathione S-transferase, and P450 activities, indicating compromised antioxidant and detoxification functions. Heat-stressed mutants showed lower critical thermal maximum, reduced survival, and increased insecticide sensitivity. Moreover, mutants exhibited significantly higher supercooling and freezing points, and elevated water loss and mortality under desiccation stress, suggesting reduced cold and drought tolerance. These findings reveal that PxCBR1-L is essential for maintaining systemic physiological homeostasis under multiple environmental stresses, and its disruption leads to broad vulnerability. This work identifies PxCBR1-L as a potential target for integrated pest management in a changing climate.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of sterile insect technique processes on the performance of tephritid flies.","authors":"Mayren Sánchez-Rosario, Jorge Toledo, Walther Enkerlin, Carlos Cáceres, Daguang Lu, Rui Pereira, Abdeljelil Bakri, Pablo Liedo","doi":"10.1111/1744-7917.70172","DOIUrl":"https://doi.org/10.1111/1744-7917.70172","url":null,"abstract":"<p><p>The sterile insect technique (SIT) is a pest control method that has been successful in controlling various species of fruit flies of economic importance worldwide. However, SIT procedures can affect the performance of mass-reared sterile fruit flies, compromising the SIT effectiveness. This review aims to identify and analyze the adverse effects associated with each step of the SIT. Special emphasis is placed on identifying critical points in the SIT process that compromise the quality and highlighting the stages that require greater optimization to improve the effectiveness of the technique. We reviewed the main scientific findings that document the impact of domestication, mass-rearing, irradiation, handling, and release on the biology, ecology, behavior, and genetic makeup of sterile males, and how they affect the performance of sterile males under natural conditions, and the effectiveness of the SIT. Although all stages of the Sterile Insect Technique (SIT) impose some degree of compromise on insect performance, domestication and mass-rearing have the most profoundly negative impact on the field performance of sterile fruit flies. As the initial and most influential stages, they largely determine the overall quality and competitiveness of released insects, exerting a stronger impact than any subsequent SIT component.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of colony management in insect mass-rearing for sterile insect technique applications.","authors":"Adly M M Abd-Alla, Anne Geiger, David Haymer, Salvador Herrero, Johannes A Jehle, Fathiya Khamis, Pablo Liedo, Anna R Malacrida, Flobert Njiokou, Thiago Mastrangelo, Soumaïla Pagabeleguem, Edwin M Ramırez-Santos, Vera I D Ros, Diego F Segura, George Tsiamis, Brian L Weiss","doi":"10.1111/1744-7917.70081","DOIUrl":"https://doi.org/10.1111/1744-7917.70081","url":null,"abstract":"<p><p>Sterile Insect Technique (SIT) applications against major insect pests and disease vectors rely on the cost-effective production of high-quality sterile males. This largely depends on the optimal management of target pest colonies by maximizing the benefits provided by a genetically rich and pathogen-free mother colony, the presence of symbiotic microorganisms, and efficient domestication, mass-rearing, irradiation, and release processes. At the same time microbial (bacteria, fungi, microsporidia, and viruses) pathogen outbreaks should be minimized or eliminated, and the use of hazardous chemicals restricted. The optimization of the colony management strategies for different SIT target insects will ensure a standardized high-quality mass-rearing process and the cost-effective production of sterile males with enhanced field performance and male mating competitiveness. The aims of the Coordinated Research Project (CRP) were to develop best practices for insect colony management for the cost-effective production of high-quality sterile males for SIT applications against major insect pests and disease vectors through a multidisciplinary approach involving entomologists, geneticists, ecologists, microbiologists, pathologists, virologists, and mass-rearing experts.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FoxO-mediated γ-carotene metabolism alters body coloration in Myzus persicae in response to starvation.","authors":"Xiucheng Xie, Yujing Liu, Yuhong Zhong, Jinming Lu, Xin Xia, Qinqin Xu, Biyue Ding, Jinjun Wang, Feng Shang, Wei Dou","doi":"10.1111/1744-7917.70169","DOIUrl":"https://doi.org/10.1111/1744-7917.70169","url":null,"abstract":"<p><p>Body color transformations play important roles in the environmental adaptations of organisms. However, the molecular mechanisms and ecological significance underlying these phenomena remain unclear. In this study, we observed that starvation induced a shift in Myzus persicae body coloration from red to green. Through transcriptomic analyses, RNA interference (RNAi) assays, and dual-luciferase assays, we demonstrated that starvation down-regulates the expression of carotene desaturase 2 (Cde2), a key gene involved in the dehydrogenation of γ-carotene, through the nutrient-responsive transcription factor FoxO, ultimately leading to a color transition in M. persicae. Metabolomic analyses further revealed that starvation enhances the cyclization of γ-carotene and the metabolism of β-carotene, which is a γ-carotene cyclization product. This metabolic change strengthens aphids' behavioral preference for yellow cues, which facilitates aphid-located host plants to combat starvation stress. Overall, these results provide new insights into the mechanisms underlying body color plasticity and environmental adaptation strategies in insects.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The binding properties of Plus-C odorant binding protein 14 in Orius sauteri to host volatiles.","authors":"Zhe-Ran Wu, Zhi-Qiang Wei, Yu-Juan Zhang, Min Lu, Xiao-Long Liu","doi":"10.1111/1744-7917.70171","DOIUrl":"https://doi.org/10.1111/1744-7917.70171","url":null,"abstract":"<p><p>Orius sauteri (Poppius) (Hemiptera: Anthocoridae) is a natural enemy of many pests, and also feeds and oviposits on plant material. Odorant binding proteins (OBPs) play an important role in the olfactory system of insects. The molecular mechanism of OBPs in O. sauteri to host volatiles is still unknown. Here, we observed by quantitative real-time polymerase chain reaction that the expression level of OsauOBP14 was primarily in the antennae. We performed a fluorescence competition binding assay, and the results showed that OsauOBP14 exhibited binding affinities to 5 host volatiles (hexanal, octanal, 1-octen-3-ol, 2-ethyl hexanol, and p-cymene). Subsequently, we conducted homology modeling, molecular docking, and site-directed mutagenesis, and the result showed that 2 key residues (W13 and R58) play a key role in the binding of OsauOBP14 to ligands. In subsequent Y-tube tests, olfactory behavioral assay revealed that hexanal was significantly attractive to male adults, octanal and 1-octen-3-ol were found to have significant attractant effects on female adults. Furthermore, RNA interference and Y-tube olfactometer confirmed that the attraction to the 3 host volatiles was significantly attenuated. Our study revealed that OsauOBP14 is involved in olfactory detection and helps to better understand the molecular mechanism of OBPs in O. sauteri to host plant volatiles.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innexin genes function in the development, metamorphosis, and reproduction of Tribolium castaneum.","authors":"Yusi Li, Xiaoqiao Huang, Meiqian Zhang, Chengjun Li, Bin Li","doi":"10.1111/1744-7917.70170","DOIUrl":"https://doi.org/10.1111/1744-7917.70170","url":null,"abstract":"<p><p>Innexins are the main components of gap junctions in invertebrates. However, the evolution and function of these in insects remain elusive. Phylogenetic analysis indicated that insect innexin genes originated from one ancestral gene, and they were divided into 6 clusters. Totally, 8 innexin (inx) genes were identified from Tribolium castaneum. These genes were highly expressed in different stages, including inx1 and inx7 in late pupae, inx2, inx3, and inx7-like2 in early pupae, shaking-B and inx7-like1 at postembryonic stages, and inx4/5/6 at early embryos and late adults. Tissue expression analysis indicated they had high transcription in distinct tissues, containing inx1 and inx7-like1 in the wing and gut of late pupae, inx7 in the gut and central nervous system of late pupae, shaking-B in most tissues except wings of late pupae, inx2 and inx3 in all tissues of early pupae, inx7-like2 in the gut and fat body of early pupae, and inx4/5/6 in the ovary of late adults. RNA interference (RNAi) experiments revealed that inx2 and inx4/5/6 were required for metamorphosis, fertility, and ovarian development, inx3 functioned during eclosion, inx1, inx7, and inx7-like1 played important roles in embryonic development, and shaking-B was critical for adult survival. Furthermore, silencing inx2, inx4/5/6, inx7, or shaking-B downregulated the expression of vitellogenin (Vg) and Vg receptor in female adults. These results indicated that innexin genes had crucial roles in the metamorphosis, fertility, and embryonic development of insects and provided the theoretical basis for developing inx2, inx3, and inx4/5/6 as potential targets of RNAi to control pests.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell microliter droplet screening microfluidic system enables high-throughput isolation and cultivation of lignin-degrading bacteria from the termite gut.","authors":"Farhan Ahmad, Syed Zeeshan Haider, Muhammad Zohaib Nawaz, Sivasamay Sethupathy, Mudasir A Dar, Jianzhong Sun, Daochen Zhu","doi":"10.1111/1744-7917.70159","DOIUrl":"https://doi.org/10.1111/1744-7917.70159","url":null,"abstract":"<p><p>Termite gut microbiome hosts diverse lignin-degrading bacteria, yet a substantial proportion remains uncultivated due to the complexity of their microbial interactions and growth requirements. In the current study, a novel single-cell microliter droplet screening microfluidic system (MISS Cell) was employed and compared with the conventional agar plate method for the high-throughput isolation and cultivation of lignin-degrading bacteria from the gut system of the higher termite Nasutitermes tiantongensis. The amplicon sequencing of 16S rRNA was conducted to assess the diversity of cultured bacteria. Compared to the conventional method, the MISS Cell system significantly improved the recovery of diverse ligninolytic bacteria, isolating 477 individual ligninolytic bacterial colonies, whereas the traditional method recovered only 73 colonies. A total of 97 operational taxonomic units (OTUs), including Acinetobacter sp. CIP_64.2, Advenella kashmirensis, and Staphylococcus sciuri, with 29 classified and 68 unclassified OTUs, were successfully obtained from both methods. The MISS Cell system yielded 16.7% more OTUs than the traditional agar plate approach. The bacterial isolates belonging to the phyla Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota, comprising 23 families, 31 genera, and 54 species, were captured using both methods. Additionally, about 8.24% of the OTUs remained unclassified at the phylum level, underscoring the need for further taxonomic characterization of termite gut microbiota. These findings emphasize that the currently known repertoire of ligninolytic bacterial species is still incomplete and demonstrate the potential of the droplet-based MISS Cell system for uncovering novel microbes with lignin-degrading capabilities.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purine nucleoside phosphorylase mediates midgut physiological functions in Aedes aegypti by orchestrating uric acid biosynthesis and oxidative stress homeostasis.","authors":"Houming Ren, Xiaolin Yang, Huixuan Tang, Xin Wang, Zhuanzhuan Su, Qingshan Ou, Yujiao Han, Yuqi Lou, Symphony Kashyap, Qian Pu, Shiping Liu","doi":"10.1111/1744-7917.70155","DOIUrl":"https://doi.org/10.1111/1744-7917.70155","url":null,"abstract":"<p><p>Insects excrete nitrogenous waste primarily through the synthesis of uric acid, with purine nucleoside phosphorylase (PNP; AAEL002269) serving as a critical enzyme in this metabolic pathway. Downregulation of PNP has been shown to significantly disrupt blood digestion, ovarian development, and adult longevity in mosquitoes following a blood meal. However, the precise molecular mechanisms underlying these phenotypic consequences remain poorly understood. In this study, we employed clustered regularly interspaced small palindromic repeats (CRISPR) / CRISPR-associated nuclease 9-mediated gene editing to knockout the PNP gene in Aedes aegypti, thereby providing further evidence for the essential biological roles of PNP. Comprehensive transcriptomic and proteomic analyses revealed 5 238 differentially expressed transcripts and 145 differentially expressed proteins in PNP-deficient mosquitoes. These molecular changes were associated with key biological processes, including oxidative stress, energy metabolism, and xenobiotic detoxification. Notably, the downregulation of uric acid synthesis pathway genes, 5'-nucleotidase II (NT2; AAEL024497), and xanthine dehydrogenase 1 (XDH1; AAEL002683), recapitulated the phenotypic defects observed in PNP-deficient mosquitoes. Further mechanistic investigations demonstrated that PNP depletion led to mitochondrial dysfunction in midgut cells, reduced expression of digestive enzymes, impaired uric acid synthesis, elevated ammonia accumulation, and increased reactive oxygen species (ROS) levels. Our results indicate that the disruption of uric acid synthesis was closely linked to ROS overproduction. PNP maintains physiological homeostasis in mosquitoes by coordinately regulating uric acid synthesis and ROS levels. This study not only advances our understanding of antioxidant defense mechanisms and nitrogen metabolism in mosquitoes but also identifies potential molecular targets for the development of novel strategies to control mosquito-borne diseases.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}