Insect Science最新文献

Mutation in Bombyx mori sericin5 gene alters silk gland growth and protein components and results in decreased secretion and performance of silk fiber. 家蚕丝胶蛋白5基因的突变改变了蚕丝腺的生长和蛋白质成分，导致蚕丝纤维的分泌和性能下降。

IF 3 1区农林科学

Insect Science Pub Date : 2026-05-08 DOI: 10.1111/1744-7917.70294

Kaiyu Guo, Shanshan Wen, Linzi Yue, Dongchao Zhao, Xiaolu Zhang, Wenbo Hu, Jingmin Duan, Zhen Xiang, Zhaoming Dong, Qingyou Xia, Ping Zhao

{"title":"Mutation in Bombyx mori sericin5 gene alters silk gland growth and protein components and results in decreased secretion and performance of silk fiber.","authors":"Kaiyu Guo, Shanshan Wen, Linzi Yue, Dongchao Zhao, Xiaolu Zhang, Wenbo Hu, Jingmin Duan, Zhen Xiang, Zhaoming Dong, Qingyou Xia, Ping Zhao","doi":"10.1111/1744-7917.70294","DOIUrl":"https://doi.org/10.1111/1744-7917.70294","url":null,"abstract":"Insects spin non-cocoon silk for protection, adhesion, and transfer with the environment. Sericin is a major component of non-cocoon silk fibers, yet our understanding of the sericin proteins is quite limited. In this study, we used CRISPR/Cas9-mediated gene editing to prepare a homozygous mutant strain that resulted in premature termination of the translation of non-cocoon sericin protein Ser5. We found that the silk glands of the Ser5 mutant (Ser5-/-) were smaller than those of the wild-type (WT), and both silk yield and major silk proteins significantly decreased in the larval stage. Seven kinds of non-cocoon silk were collected, and we found that the morphology of silk did not vary, but the diameter of the silk was significantly reduced in Ser5-/-. Further research revealed that the adhesive strength of native silk dope in Ser5-/- was significantly lower than that of the WT silkworm. Proteomic data indicated that the autophagy and apoptosis proteins increased significantly in Ser5-/-; differentially expressed proteins were enriched in pathways related to cellular stress responses and transcription and translation. Detection of the autophagy-related gene ATG8 also indicated that knockout of the Ser5 gene may lead to a level of cell stress, thus affecting the synthesis and secretion of silk proteins. Our study highlights the importance of the silk sericin gene in silk formation, silk protein adhesion, and the cellular developmental processes of the silkworm. These findings enhance our understanding of the functional roles of sericin genes in insects and provide a foundation for the development of sericin-based biomaterials.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenerational effects of adult heat stress on life history traits and population size of the seed beetle Megabruchidius dorsalis: Implications for adaptive responses and pest outbreaks under climate warming. 成虫热胁迫对桔斑蝶生活史性状和种群规模的跨代影响：气候变暖条件下的适应性响应和虫害暴发

IF 3 1区农林科学

Insect Science Pub Date : 2026-05-08 DOI: 10.1111/1744-7917.70295

Jun Liao, Fang-Ling Xu, Jian-Feng Liu, Tao Wang, Guy Smagghe, Mao-Fa Yang, Cheng-Xu Wu

{"title":"Transgenerational effects of adult heat stress on life history traits and population size of the seed beetle Megabruchidius dorsalis: Implications for adaptive responses and pest outbreaks under climate warming.","authors":"Jun Liao, Fang-Ling Xu, Jian-Feng Liu, Tao Wang, Guy Smagghe, Mao-Fa Yang, Cheng-Xu Wu","doi":"10.1111/1744-7917.70295","DOIUrl":"https://doi.org/10.1111/1744-7917.70295","url":null,"abstract":"The seed beetle Megabruchidius dorsalis (Fåhraeus) is a major oligophagous boring pest of Gleditsia seeds, and its responses to environmental stressors are critical for understanding its potential distribution and population dynamics under climate change. We examined the transgenerational effects of adult heat stress on the development and demographic parameters of F1 offspring using an age-stage, two-sex life table approach. The results showed that the egg development was accelerated and the boring stage was shortened after parental heat stress, while reducing hatching success. Furthermore, stress duration had a significant impact on adult longevity. After 1 h of parental exposure at 40 and 45 °C, the adult longevity of the offspring was shortened. However, after 2 and 3 h of exposure at the same temperatures, the adult longevity was extended. Exposure of adults to 35 and 40 °C for 1 h significantly increased the intrinsic rate of increase (r) and finite rate of increase (λ) in their offspring, suggesting enhanced short-term population growth under moderate heat stress. In contrast, prolonged exposure (2-3 h) of 30-45 °C reduced net reproductive rate (R0) and mean fecundity (F), indicating potential long-term fitness costs. Overall, adult heat stress led to shorter generation times and advanced reproduction in offspring, but at the cost of reduced reproductive output. These findings highlight the dual role of heat stress in shaping M. dorsalis life history strategies, providing new insights into insect adaptation to thermal stress and offering a theoretical basis for predicting its population dynamics and pest outbreaks under ongoing climate warming.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Siobla longitheca: a new sawfly species specializing on the invasive Phytolacca americana. 长锯蝇：一种新的锯蝇种，专指入侵的美洲植蝇。

IF 3 1区农林科学

Insect Science Pub Date : 2026-05-05 DOI: 10.1111/1744-7917.70290

Hongdi Shi, Danfeng Liu, Xiao Xu, Yi Wang, Yupeng Geng, Meicai Wei, Gengyun Niu, Bo Li

引用次数: 0

Probiotic-based fermentation of watermelon waste: Effects on bioconversion efficiency, microbial shifts, and expression profiles of black soldier fly larvae. 西瓜废弃物益生菌发酵：对黑虻幼虫生物转化效率、微生物迁移和表达谱的影响

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-28 DOI: 10.1111/1744-7917.70280

Fareed Uddin Memon, Sheraz Ahmad, Qianru Mo, Song Liu, Xiaole Xie, Farhan Nabi, Zhijun Huang, Gianluca Tettamanti, Ling Tian

{"title":"Probiotic-based fermentation of watermelon waste: Effects on bioconversion efficiency, microbial shifts, and expression profiles of black soldier fly larvae.","authors":"Fareed Uddin Memon, Sheraz Ahmad, Qianru Mo, Song Liu, Xiaole Xie, Farhan Nabi, Zhijun Huang, Gianluca Tettamanti, Ling Tian","doi":"10.1111/1744-7917.70280","DOIUrl":"https://doi.org/10.1111/1744-7917.70280","url":null,"abstract":"Insects such as black soldier fly larvae (Hermetia illucens, BSFL) are efficient bioconverters whose growth and physiological performance are strongly influenced by diet composition, gut microbiota, and the molecular regulation. This study investigated how a probiotic-based fermentation strategy modulates larval physiology, microbiome dynamics, and gene expression when BSFL are reared on fermented watermelon waste. Watermelon waste was fermented for 14 d using a consortium of Bacillus subtilis, Enterococcus faecalis, and Aspergillus oryzae, resulting in a nutritionally enhanced substrate. BSFL fed on fermented diet exhibited significantly increased growth performance, biomass yield, and nutritional content of the insect biomass. Metagenomic analysis revealed marked enrichment of gut microbes belonging to genera known to include beneficial and commensal species (Enterococcus, Vagococcus, Carnobacterium, Tetragenococcus, and Blautia) along with a reduction in genera containing species previously associated with opportunistic or pathogenic traits (Mycobacterium, Pseudomonas, Morganella, Pedobacter, and Serpula), indicating diet-induced modulation of host-microbe interactions. Transcriptomic profiling highlighted an upregulation of key genes involved in growth and development (CK1, HIB, and PDK1), protein and fat biosynthesis (DVL, GSK3, and Lpin), and immune defense (PGRP-SA, Spz, Toll, and Cactus). Functional enrichment analysis further confirmed their participation in critical signaling pathways, including Hedgehog, Wnt, mTOR, Toll and Imd, and MAPK. Overall, this study demonstrates that probiotic fermentation improves nutrient utilization, regulates host-microbe interactions, and activates molecular pathways associated with growth and immune resilience in BSFL, providing new insights into the physiological and molecular basis of dietary adaptation in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoform-specific roles of Laccase-2 in cuticle renewal and wing morphogenesis of the cigarette beetle Lasioderma serricorne. 漆酶-2在烟甲虫角质层更新和翅膀形态发生中的作用。

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-24 DOI: 10.1111/1744-7917.70292

Kang-Kang Xu, Yang-Fan Zhou, Wen-Jia Yang, Fang-Hao Wan, Guy Smagghe, Can Li

{"title":"Isoform-specific roles of Laccase-2 in cuticle renewal and wing morphogenesis of the cigarette beetle Lasioderma serricorne.","authors":"Kang-Kang Xu, Yang-Fan Zhou, Wen-Jia Yang, Fang-Hao Wan, Guy Smagghe, Can Li","doi":"10.1111/1744-7917.70292","DOIUrl":"https://doi.org/10.1111/1744-7917.70292","url":null,"abstract":"Laccase-2 (Lac2) is a key phenoloxidase that catalyzes cuticular sclerotization and pigmentation, which are processes essential for insect molting and metamorphosis. Although the alternatively spliced isoform Lac2A is known to function in cuticular tanning, the physiological role of Lac2B has remained largely unresolved. Here, we identified and functionally characterized two Lac2 isoforms, LsLac2A and LsLac2B, from the cigarette beetle Lasioderma serricorne, a globally important stored-product pest. Both isoforms are highly expressed during the pupal and eclosion stages, with transcripts localized mainly in the cuticle and wing tissues, and are transcriptionally induced by 20-hydroxyecdysone (20E), linking their activity to endocrine regulation of development. Functional analyses revealed that LsLac2A and LsLac2B have distinct and complementary roles in molting and metamorphosis. During larval molting, LsLac2A promotes the synthesis of new cuticle by regulating genes involved in cuticular protein and chitin biosynthesis, whereas LsLac2B facilitates degradation of the old cuticle through modulation of chitinolytic and cuticular protein-degrading genes. In the pupal stage, both isoforms influence elytral pigmentation, sclerotization, and wing morphogenesis via the coordinated regulation of wing development-related and yellow family genes. These results reveal isoform-specific functions of LsLac2 in cuticle renewal and morphogenesis, uncovering a novel mechanism of hormonal and genetic integration during insect development. This work provides new insight into the molecular specialization of Lac2 and identifies promising RNAi-based targets for the sustainable control of L. serricorne.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel function of arylalkalamine N-acetyltransferase-1 in modulating host coagulation and blood feeding in Aedes aegypti. 芳基碱胺n -乙酰转移酶-1在调节埃及伊蚊宿主凝血和血液摄食中的新功能

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-24 DOI: 10.1111/1744-7917.70288

Xue Gong, Dingfeng Duan, Xiaojing Zhu, Yaneng Huang, Jiukai Chen, Linlong Jiang, Lei Zhang, Qian Han, Chenghong Liao

{"title":"Novel function of arylalkalamine N-acetyltransferase-1 in modulating host coagulation and blood feeding in Aedes aegypti.","authors":"Xue Gong, Dingfeng Duan, Xiaojing Zhu, Yaneng Huang, Jiukai Chen, Linlong Jiang, Lei Zhang, Qian Han, Chenghong Liao","doi":"10.1111/1744-7917.70288","DOIUrl":"https://doi.org/10.1111/1744-7917.70288","url":null,"abstract":"During blood feeding, mosquitoes modulate the host's coagulation response via salivary proteins to facilitate their blood intake. Arylalkylamine N-acetyltransferase-1 (aaNAT1) exhibits significant expression levels in the salivary glands of Aedes aegypti. While AaaaNAT1 deletion impairs anticoagulant function, the underlying mechanism has remained elusive. Here we combined in vitro and in vivo experiments to elucidate this mechanism. Recombinant AaaaNAT1, expressed in an E. coli-based prokaryotic system, dose-dependently suppressed human platelet aggregation and prolonged both clot-time and fibrinolysis in calibrated thromboelastography assays. When the AaaaNAT1 protein is injected into mice via intramuscular injection, it will rapidly acetylate the norepinephrine in the surrounding tissue microenvironment, thereby inhibiting platelet aggregation. Meanwhile, RNAi-mediated silencing of AaaaNAT1 in female mosquitoes reduced endogenous octopamine levels, downregulated the octopamine receptor, and more than doubled the time required to locate a host and complete feeding. We have uncovered a dual-function strategy that inhibits mosquito blood-sucking: dual modulation of host hemostasis and vector behavior by a single enzyme. Our findings demonstrate that AaaaNAT1 functions as a salivary anticoagulant by acetylating norepinephrine while simultaneously governing host-seeking via octopamine signaling. This establishes AaaaNAT1 as a unique target that can be exploited for next-generation vector control, extending its canonical roles beyond pigment synthesis and immunity to a novel, druggable node for vector control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bombyx mori BmLis1 inhibits BmNPV replication by promoting the cell cycle G2/M progression. 家蚕bmis1通过促进细胞周期G2/M进程抑制BmNPV复制。

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-24 DOI: 10.1111/1744-7917.70291

Feifei Liu, Ya Zhang, Jie Wang, Yunxiang Jia, Ying Ma, Peng Chen, Cheng Lu, Zhanqi Dong, Minhui Pan

{"title":"Bombyx mori BmLis1 inhibits BmNPV replication by promoting the cell cycle G2/M progression.","authors":"Feifei Liu, Ya Zhang, Jie Wang, Yunxiang Jia, Ying Ma, Peng Chen, Cheng Lu, Zhanqi Dong, Minhui Pan","doi":"10.1111/1744-7917.70291","DOIUrl":"https://doi.org/10.1111/1744-7917.70291","url":null,"abstract":"Lissencephaly-1 (Lis1) is a highly conserved cytoplasmic dynein regulator found in eukaryotes. It is involved in regulating processes such as cell proliferation, spindle orientation, nuclear migration, and microtubule-dependent transport. Baculovirus frequently hijacks these cellular processes to facilitate infection and replication, whether Lis1 has a role during Baculovirus infection remains unexplored. This study found that Bombyx mori nucleopolyhedrovirus (BmNPV) infection downregulates Bombyx mori Lis1 (BmLis1) expression, suggesting its involvement in regulating viral replication. To investigate the function of BmLis1 during BmNPV infection, we performed flow cytometry, Western blotting, and qRT-PCR analyses following BmLis1 overexpression and knockout. All results demonstrated that BmLis1 significantly suppresses BmNPV replication, as evidenced by changes in the expression levels of EGFP-positive cells, VP39 protein, and key viral genes. Meanwhile, we demonstrated that BmLis1 significantly improves survival and inhibits viral proliferation in the BmLis1-OE transgenic line. Moreover, we proved that BmLis1 inhibits viral replication by upregulating BmCyclin B expression, thereby reducing the proportion of cells in the G2/M phase of the cell cycle. More importantly, we found that BmNude1, an interacting protein of BmLis1, acts synergistically with BmLis1 to influence BmNPV proliferation by modulating BmCyclin B expression and G2/M phase progression. Collectively, these findings provide the first evidence that BmLis1 is a crucial host restriction factor against BmNPV infection that involves regulating cell cycle progression. These findings provide novel insights into the molecular mechanisms by which host factors combat baculovirus infection through modulation of the cell cycle.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota is associated with circadian rhythms and clock gene expression in honeybees. 肠道微生物群与蜜蜂的昼夜节律和时钟基因表达有关。

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-24 DOI: 10.1111/1744-7917.70287

Yueyi Wang, Xiaofei Wang, Hao Zheng, Yiyuan Li

{"title":"Gut microbiota is associated with circadian rhythms and clock gene expression in honeybees.","authors":"Yueyi Wang, Xiaofei Wang, Hao Zheng, Yiyuan Li","doi":"10.1111/1744-7917.70287","DOIUrl":"https://doi.org/10.1111/1744-7917.70287","url":null,"abstract":"To accommodate daily environmental changes, insects exhibit species-specific circadian rhythms in behavior and physiology. Compared to non-social insects, honeybees display robust, socially regulated circadian rhythms aligned with colony tasks, while bumblebees show more flexible rhythms linked to simpler social structures. The gut microbiota has been proposed to influence circadian rhythms in mammals through microbial metabolites, hormone regulation, and gene expression. However, its role in honeybee and bumblebee circadian rhythms remains unclear. In this study, we generated gnotobiotic honeybees and bumblebees to explore microbiota-driven circadian modulation through behavioral and transcriptional analyses. Behavioral assays showed that gut microbiota had no significant effect on bumblebee locomotor activity, rhythmicity strength, or free-running period under the tested conditions. In contrast, microbiota colonization in honeybees led to increased daily activity, enhanced rhythmicity strength, and stronger circadian oscillations without affecting the intrinsic free-running period. Notably, qPCR analysis revealed that, at T5, colonized bees exhibited higher per expression together with reduced cwo and serotonin receptor 5-ht1 expression in the honeybee brain. These transcriptional changes indicate potential links between the gut microbiota and honeybee circadian rhythms via the gut-brain axis, possibly involving altered serotonergic signaling. Overall, our results indicate an association between gut microbiota and circadian rhythmicity in honeybees, whereas bumblebees showed little responsiveness, suggesting potential species-specific interactions between microbial signals and host circadian systems. This study advances understanding of microbiota-driven behavioral regulation in eusocial insects and lays the groundwork for future mechanistic investigations.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CCAP receptor 2, rather than CCAPR1, functions in pupal ecdysis in the 28-spotted larger potato ladybird. CCAP受体2，而不是CCAPR1，在28斑马铃薯瓢虫的蛹蜕皮中起作用。

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-20 DOI: 10.1111/1744-7917.70289

Yi-Kuan Wu, Nan Wu, Ya-Hao Rao, Wei-Kang Zheng, Man-Yu Sun, Lin Jin, Guo-Qing Li

{"title":"CCAP receptor 2, rather than CCAPR1, functions in pupal ecdysis in the 28-spotted larger potato ladybird.","authors":"Yi-Kuan Wu, Nan Wu, Ya-Hao Rao, Wei-Kang Zheng, Man-Yu Sun, Lin Jin, Guo-Qing Li","doi":"10.1111/1744-7917.70289","DOIUrl":"https://doi.org/10.1111/1744-7917.70289","url":null,"abstract":"Crustacean cardioactive peptide receptor (CCAPR), a G protein-coupled receptor, is critical for pupal ecdysis in Drosophila melanogaster and Acyrthosiphon pisum, both of which possess a single ccapr gene. In this study, we identified two ccapr genes in Henosepilachna vigintioctomaculata, a potato-defoliating ladybird widely distributed in Asia, and found that the two receptors have diverged functionally in their requirement for pupal ecdysis. Bayesian phylogenetic analysis recovered well-supported within-lineage relationships and placed HvCCAPR1 and HvCCAPR2 as a sister pair within the coleopteran clade. Spatiotemporal expression analysis showed that Hvccapr2 was upregulated immediately before the onset of pupal ecdysis and exhibited a stronger bias toward neural and muscular tissues. Injection of in vitro-synthesized dsRNA or oral administration of bacterially expressed dsRNA targeting the common region shared by both genes, as well as dsccapr2 but not dsccapr1, impaired larval-pupal transition. Moreover, knockdown of Hvccapr1 + Hvccapr2 or Hvccapr2, but not Hvccapr1, markedly prolonged the durations of ecdysis and postecdysis behavioral sequences. As a consequence, most Hvccapr1 + Hvccapr2 and Hvccapr2 RNAi prepupae failed to shed the old larval cuticle. In contrast, pupal cuticles beneath the retained larval exoskeleton became pigmented in developmentally arrested Hvccapr1 + Hvccapr2 and Hvccapr2 RNAi beetles. Together, these findings demonstrate that CCAPR2, rather than CCAPR1, plays the predominant role in coordinating pupal ecdysis behavior in H. vigintioctomaculata.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between the wood-boring pest Anoplophora glabripennis and pathogen Fusarium solani in the native range. 原生地蛀木害虫光肩天牛与病原菌枯萎病的关系。

IF 3 1区农林科学

Insect Science Pub Date : 2026-04-19 DOI: 10.1111/1744-7917.70286

Li-Xiang Wang, Chun-Chun Li, Xuan Wang, You-Qing Luo, Ning Lv, Ning-Ning Fu, Jia-Yi Tang

{"title":"The relationship between the wood-boring pest Anoplophora glabripennis and pathogen Fusarium solani in the native range.","authors":"Li-Xiang Wang, Chun-Chun Li, Xuan Wang, You-Qing Luo, Ning Lv, Ning-Ning Fu, Jia-Yi Tang","doi":"10.1111/1744-7917.70286","DOIUrl":"https://doi.org/10.1111/1744-7917.70286","url":null,"abstract":"Wood-feeding insects often rely on microbial symbionts to thrive on nutrient-poor xylem. Anoplophora glabripennis is a wood-boring pest that inhabits a wide range of healthy deciduous hosts. The fungus Fusarium solani is associated with A. glabripennis. This study investigated their relationship in the native range of A. glabripennis, and evaluated how F. solani is carried and transmitted, as well as the phylogenetic relationship of F. solani species complex (FSSC) populations from different countries. Fungal communities differed among eggs carried by adult, oviposition secretions, healthy phloem adjacent to the oviposition pit, and soft rot phloem consumed by newly hatched larvae; but were similar in eggs and secretions. F. solani was highly enriched in eggs (93.07%), oviposition secretions (86.39%), and soft rot phloem (63.44%), but was absent in healthy phloem. The F. solani isolation rate from oviposition pits was 100% across different hosts and locations, and it was found in larval guts and frass at all life stages. In addition, GFP-labeled F. solani was only detected in larval guts (10, 40, 60 days post-feeding), but not in the fat body or epidermal tissue. Newly hatched larvae had the highest FSSC-specific copy numbers in their guts than those at other life stages. FSSC isolated from the gut of A. glabripennis in China forms a separate clade, with a relatively distant genetic relationship to the United States larval isolates. These results support the symbiotic relationship between A. glabripennis and F. solani, and demonstrate that F. solani is transmitted via female adult oviposition and carried in the guts by larval feeding.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0