Insect Science最新文献

Gut microbiota shape gene regulatory networks to direct intestinal stem cell differentiation trajectories in Apis mellifera. 肠道微生物群塑造基因调控网络以指导蜜蜂肠道干细胞分化轨迹。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-11 DOI: 10.1111/1744-7917.70157

Huiling Liu, Liang Meng, Jie Ma, Bingkun Yang, Hao Wang, Xinyu Li, Xiaohuan Mu, Li Zheng, Hao Chen, Yan Liu, Xiaofei Wang, Yifan Zhai, Shanshan Liu, Hao Zheng

{"title":"Gut microbiota shape gene regulatory networks to direct intestinal stem cell differentiation trajectories in Apis mellifera.","authors":"Huiling Liu, Liang Meng, Jie Ma, Bingkun Yang, Hao Wang, Xinyu Li, Xiaohuan Mu, Li Zheng, Hao Chen, Yan Liu, Xiaofei Wang, Yifan Zhai, Shanshan Liu, Hao Zheng","doi":"10.1111/1744-7917.70157","DOIUrl":"https://doi.org/10.1111/1744-7917.70157","url":null,"abstract":"Honeybees are key pollinators of flowering plants, and their health is closely associated with gut function. The function is regionally specialized, with nutrient absorption distributed across distinct gut compartments and influenced by the gut microbiota. Here, we constructed a single-nucleus transcriptomic atlas of the honeybee gut under microbiota-free and conventional (CV) conditions and uncovered a microbiota-dependent mechanism regulating intestinal homeostasis. We characterized major intestinal cell types in the honeybee, including intestinal stem cell/enteroblast (ISC/EB), enterocyte (EC), enteroendocrine cell (EE), visceral muscle, and enteric neurons. The cellular data uncovered that regional functional specialization of the intestine is associated with the spatial distribution of EC and EE cell subtypes. Intriguingly, we found that microbiota significantly alter cell composition and gene expression in the ileum, particularly within the ISC/EB population. Relish, a conserved nuclear factor-κB transcription factor in the immune deficiency pathway, was upregulated in the ISC/EB within the ileum of CV bees. The gene regulatory network (GRN) revealed that the Relish regulon is explicitly activated in ISC/EB of CV bees, promoting ISC/EB toward ECs by directly regulating differentiation factors.Our study highlights the cellular and regional complexity of the honeybee gut and demonstrates that region-specific microbiota may modulate GRN in ISC/EBs, thereby shaping epithelial cell composition and maintaining intestinal homeostasis. This work provides a valuable resource for understanding host-microbiota interactions in honeybees and serves as a reference to study gut function, immune regulation, and bee health at single-cell resolution.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV2 and LW opsin genes mediate phototactic responses in the Asian lady beetle, Harmonia axyridis. UV2和LW视蛋白基因介导亚洲瓢虫的光致反应。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-11 DOI: 10.1111/1744-7917.70147

Ke-Rui Qiu, Kou Wang, Yi-Fei Dai, Hao-Min An, Qiu-Ying Huang, Fen Zhu, Wen Liu, Xiao-Ping Wang

{"title":"UV2 and LW opsin genes mediate phototactic responses in the Asian lady beetle, Harmonia axyridis.","authors":"Ke-Rui Qiu, Kou Wang, Yi-Fei Dai, Hao-Min An, Qiu-Ying Huang, Fen Zhu, Wen Liu, Xiao-Ping Wang","doi":"10.1111/1744-7917.70147","DOIUrl":"https://doi.org/10.1111/1744-7917.70147","url":null,"abstract":"Phototaxis is a critical behavior in insects and is closely linked to vision and environmental adaptation. Understanding how insects perceive light and exhibit phototactic responses is crucial for assessing the ecological impact of artificial light at night. However, the molecular and neural mechanisms that regulate phototactic responses in insects remain largely unknown. Here, we investigated the phototactic response of the Asian lady beetle Harmonia axyridis using wavelength-specific behavioral assays, combined with gene RNA interference (RNAi) and pharmacological rescue experiments. Our results indicated that adult beetles exhibited phototactic response peaks at 365, 420, and 520 nm. Knockdown of opsin genes UV1, UV2, and LW demonstrated that UV2 and LW play essential roles in wavelength-specific phototactic responses, while no specific role for UV1 has been identified in the adult phototactic response. Knockdown of one opsin gene affected the expression of the other opsin genes, especially highlighting a regulatory interaction between UV2 and LW. Histidine decarboxylase (HDC) is responsible for the synthesis of histamine. HDC knockdown impaired phototactic responses without affecting locomotor activity. Exogenous histamine successfully rescued the phototactic response in HDC-RNAi adults, confirming that histamine was required for visual signal transduction. Furthermore, UV2 or LW knockdown significantly reduced HDC expression, suggesting an interaction between photoreception and histaminergic signaling. These findings suggest that UV2 and LW play key roles in the phototactic response and that histamine is required for visual signal transduction in H. axyridis. This study provides insights into the visual and neuromodulatory pathways that drive adult phototactic behavior in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of pupal color-based genetic sexing strains in Anastrepha obliqua (Diptera: Tephritidae). 双翅目：绢蝇科蛹色遗传性别鉴定菌株的建立与评价。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-08 DOI: 10.1111/1744-7917.70163

Daisy P Cárdenas-Enríquez, Víctor García-Martínez, Jorge Ibáñez-Palacios, Brenda Torres-Huerta, Maria F Ruiz-Pérez, José S Meza

{"title":"Development and evaluation of pupal color-based genetic sexing strains in Anastrepha obliqua (Diptera: Tephritidae).","authors":"Daisy P Cárdenas-Enríquez, Víctor García-Martínez, Jorge Ibáñez-Palacios, Brenda Torres-Huerta, Maria F Ruiz-Pérez, José S Meza","doi":"10.1111/1744-7917.70163","DOIUrl":"https://doi.org/10.1111/1744-7917.70163","url":null,"abstract":"Anastrepha obliqua, a neotropical pest widely distributed in the Americas, attacks mango and other tropical fruits. In Mexico, it is controlled through integrated pest management, using the Sterile Insect Technique (SIT) as a main component. The applicability of SIT is significantly improved with the use of genetic sexing strains (GSS) that allow the possibility to release exclusively sterile males, the primary component of the technique. This study reports the isolation and characterization of two pupal mutations: black pupae (bp) and sphere pupae (sp), allowing for the first time the development of a genetic sexing system based on pupal color in this species. Inheritance analyses from reciprocal crosses between wild-type and mutant individuals showed F2 phenotypic segregation consistent with a recessive Mendelian inheritance pattern, and linkage analysis indicated that the bp and sp loci are in separate chromosomes. Using the bp mutation, two GSS were developed through gamma irradiation [T(Y;bp+)/bp-22](GSS-22) and ethyl methanesulfonate treatment [T(Y;bp+)/bp-354](GSS-354). Both GSS exhibited sex-specific pupal differentiation but displayed a high frequency of recombinants. Despite an initial reduction in biological fitness, GSS-22 demonstrated greater genetic stability and a lower frequency of recombinants than GSS-354. Discrepancies between cytogenetic and genomic data, particularly regarding the localization of the gene responsible for the black pupae phenotype, underscore the need to integrate polytene chromosome and genomic analyses to characterize these translocations and improve GSS stability precisely. These results represent a breakthrough in the creation of genetic tools for the management of A. obliqua control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic benefits conferred by duplication of the facilitated trehalose transporter in Lepidoptera. 鳞翅目易化海藻糖转运体的复制所带来的代谢益处。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-08 DOI: 10.1111/1744-7917.70121

Tao Zhong, Guosheng Hua, Chaojie Chen, Guanwang Shen, Zhiqing Li, Xiaoting Hua, Ping Lin, Ping Zhao, Qingyou Xia

{"title":"Metabolic benefits conferred by duplication of the facilitated trehalose transporter in Lepidoptera.","authors":"Tao Zhong, Guosheng Hua, Chaojie Chen, Guanwang Shen, Zhiqing Li, Xiaoting Hua, Ping Lin, Ping Zhao, Qingyou Xia","doi":"10.1111/1744-7917.70121","DOIUrl":"https://doi.org/10.1111/1744-7917.70121","url":null,"abstract":"In addition to being one of the primary processes for the formation and expansion of gene families, gene duplication also establishes the basis for the diversity and redundancy of gene functions, providing an abundance of genetic resources and a potent adaptive potential for biological evolution. Trehalose is a high-quality carbon source and blood sugar in insects. However, recent theoretical developments suggest that mechanisms for facilitated trehalose transport in lepidopteran insects remain relatively scarce. In this research, we identified, characterized, and analyzed the evolution of the facilitated trehalose transporter gene family in the silkworm. The results revealed that Bombyx mori and Spodoptera frugiperda experienced a significant expansion of the facilitated trehalose transporter gene (Tret) in Lepidoptera. Leveraging CRISPR/Cas9 gene editing technology, we altered BmTret60, the 60th member of this gene family, in the silkworm to investigate the gene's functional characteristics. Males and females of the BmTret60 mutant larvae were shown to exhibit a notable differentiation process in growth and developmental phases despite the fact that the larvae displayed basic viability. The silkworm's development process is influenced by BmTret60, a crucial member of the facilitated trehalose transporter gene family that controls metabolic equilibrium in fat body and hemolymph of terminal larvae. These results highlight the critical role that BmTret60, a member of the BmTret family, plays in preserving growth and homeostasis, as well as its possible role in preserving protein synthesis. Our research offers fresh perspectives on the critical function of the BmTret gene family in preserving metabolic homeostasis.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequence and expression analysis of potential spermatogenesis-specific gene cognates in the Caribbean fruit fly, Anastrepha suspensa. 加勒比果蝇精子发生特异性基因同源物的序列与表达分析。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-08 DOI: 10.1111/1744-7917.70161

Alfred M Handler, Richard B Furlong, Chao Chen, Daniel A Hahn

{"title":"Sequence and expression analysis of potential spermatogenesis-specific gene cognates in the Caribbean fruit fly, Anastrepha suspensa.","authors":"Alfred M Handler, Richard B Furlong, Chao Chen, Daniel A Hahn","doi":"10.1111/1744-7917.70161","DOIUrl":"https://doi.org/10.1111/1744-7917.70161","url":null,"abstract":"The sterile insect technique (SIT) is a highly effective biologically-based method for the suppression of many insect pest populations. SIT efficacy could be improved by methods of male sterilization that avoid the use of irradiation that can result in diminished fitness and mating competitiveness. Alternative sterilization methods include conditional disruption of genes for male fertility, or using their sperm-specific promoters to drive the expression of genes for lethal effectors. Testing has begun for the testis-specific β2-tubulin gene, though additional male fertility genes are required for redundancy or replacement, and for species where the β2-tubulin isoform does not exist or is not testis-specific. Here we had the goal of identifying and characterizing the sequence and transcriptional expression of two genes in the caribfly, Anastrepha suspensa, that are cognates of D. melanogaster spermatocyte-specific male fertility genes. In Drosophila, wampa encodes a coiled-coil dynein subunit required for axonemal assembly essential to microtubule-based sperm motility, while Prosα6T is a proteasome subunit gene required for spermatid individualization and nuclear maturation. In A. suspensa a cognate to wampa exhibited testis-specific transcript expression, which was minimal in both male and female body tissue. A Prosα6T cognate was not apparent in A. suspensa, but its constitutive isoform, Prosα6, expresses in male testes, but also in male and female body tissue. Thus, for A. suspensa, wampa remains a strong candidate gene for male sterility strategies for SIT including a direct target for gene-editing knockout and use of its promoter for testis-specific toxicity or cell death in conditional expression systems.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the interaction between Lactobacillus casei and hydrolyzed yeast on the sexual competitiveness, survival, and fecundity of Anastrepha obliqua (Diptera: Tephritidae). 干酪乳杆菌与水解酵母菌互作对斜斑绢蝇（双翅目：绢蝇科）性竞争、存活和繁殖力的影响。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-08 DOI: 10.1111/1744-7917.70162

Damara Olea, Linnet Roque-Romero, Karina Guillén-Navarro, Pablo Liedo

{"title":"Effect of the interaction between Lactobacillus casei and hydrolyzed yeast on the sexual competitiveness, survival, and fecundity of Anastrepha obliqua (Diptera: Tephritidae).","authors":"Damara Olea, Linnet Roque-Romero, Karina Guillén-Navarro, Pablo Liedo","doi":"10.1111/1744-7917.70162","DOIUrl":"https://doi.org/10.1111/1744-7917.70162","url":null,"abstract":"Enriching the diets of sterile fruit flies with bacterial species prior to their release has been shown to improve their survival and sexual competitiveness. However, most of the bacteria associated with fruit flies are enterobacteria, and some species have been classified as opportunistic pathogens. On the other hand, in diets that include hydrolyzed yeast, the effect of bacteria has been counterproductive, which could be due to the protein content in the diet being too high. Therefore, our aim here was to evaluate the effect of Lactobacillus casei, interacting with different contents of hydrolyzed yeast in the diet, on the performance of Anastrepha obliqua adults. In sexual competitiveness tests, the highest proportion of matings was achieved by males fed on a standard diet (3 : 1 sugar : yeast) plus L. casei. However, the differences with the 3 : 1 sugar : yeast diet was not significant. In survival tests, the 3 : 1 diet, without L. casei, showed the highest survival. The group fed with sugar plus L. casei showed the lowest survival. Females fed the 3 : 1 diet showed the highest fecundity, followed by those fed the 3 : 1 diet plus L. casei. Enriching the standard 3 : 1 diet with L. casei did not lead to significant improvements in male sexual performance, survival of either sex, or fecundity, compared to flies fed the standard diet alone. However, given that Lactobacillus is harmless to both humans and flies, further research into its potential application in the sterile insect technique (SIT) is recommended. Future studies should explore alternative formulations, varying concentrations, and other species within the Lactobacillus genus that may exert more pronounced effects on the biological traits of this fruit fly.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophysiological identification of 4 macrocyclic lactones as female-specific volatiles of the agarwood tree defoliator Heortia vitessoides (Lepidoptera: Crambidae). 沉香叶蝉雌性挥发物4种大环内酯的电生理鉴定。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-07 DOI: 10.1111/1744-7917.70152

Chonghe Wang, Yunwei Ju, Longbao Wang, Jiao Liu, Jie Gao, Mingxia Jin, Jin Chen, Ping Wen

{"title":"Electrophysiological identification of 4 macrocyclic lactones as female-specific volatiles of the agarwood tree defoliator Heortia vitessoides (Lepidoptera: Crambidae).","authors":"Chonghe Wang, Yunwei Ju, Longbao Wang, Jiao Liu, Jie Gao, Mingxia Jin, Jin Chen, Ping Wen","doi":"10.1111/1744-7917.70152","DOIUrl":"https://doi.org/10.1111/1744-7917.70152","url":null,"abstract":"Agarwood trees (Aquilaria spp.) are widely cultivated in tropical Asia for their valuable resin. The defoliator moth Heortia vitessoides Moore (Lepidoptera: Crambidae) is a devastating pest that significantly limits the productivity of agarwood plantations. Sex pheromones offer a sustainable and efficient approach for monitoring and controlling this pest. In this study, we report the identification of female-specific volatiles (FVs) as candidate sex pheromones produced by the female of H. vitessoides. The FVs were identified and extracted from the 3rd to 5th abdominal segments of the calling females. We combined gas chromatography coupled with mass spectrometry (GC-MS) and GC coupled with electroantennographic detection (GC-EAD) to analyze the components of the FVs in H. vitessoides. Four EAD active compounds were specific to the female abdomen, with 2 being the major components. By comparing retention times and mass spectra with synthetic racemic standards, the primary components were determined to be 4 macrocyclic lactones: 14-methyloxacyclotetradecan-2-one (A), oxacyclopentadecan-2-one (B), 16-methyloxacyclohexadecan-2-one (C), and oxacycloheptadecan-2-one (D). Further analysis using chiral standards revealed that compounds A and C possess the S configuration. GC-EAD tests on a chiral column demonstrated that all enantiomers of A and C elicited antennal responses in males, with stronger responses to the naturally occurring S enantiomers. In the choice wind tunnel assay, synthetic compounds tested individually or in blend attracted or repelled males as calling females. This study represents the first identification of macrocyclic lactones as sex pheromone candidate FVs in Lepidoptera.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics reveal developmental stage-specific adaptations of Tropilaelaps mercedesae to the honeybee host. 多组学揭示了Tropilaelaps mercedesae对蜜蜂宿主的发育阶段特异性适应。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-07 DOI: 10.1111/1744-7917.70165

Jiangli Wu, Qiaohong Wei, Jiajing Sun, Weipeng Kang, Fengying Liu, Meijiao Zhao, Shufa Xu, Bin Han

{"title":"Multi-omics reveal developmental stage-specific adaptations of Tropilaelaps mercedesae to the honeybee host.","authors":"Jiangli Wu, Qiaohong Wei, Jiajing Sun, Weipeng Kang, Fengying Liu, Meijiao Zhao, Shufa Xu, Bin Han","doi":"10.1111/1744-7917.70165","DOIUrl":"https://doi.org/10.1111/1744-7917.70165","url":null,"abstract":"The ectoparasitic honeybee (Apis mellifera) mite Tropilaelaps mercedesae represents a serious threat to Asian apiculture and a growing concern for global beekeeping due to its high reproductive capacity and host adaptability. However, the regulatory mechanisms underlying its host adaptation across life stages remain poorly characterized. Here, we performed integrated transcriptomic, proteomic, and metabolomic analyses of female mites at 4 key postembryonic developmental stages: protonymphs, deutonymphs, mature adults, and reproductive adults. Our multi-omics approach reveals distinct stage-specific regulatory programs. Nymphal stages exhibit coordinated activation of chitin metabolism pathways for structural growth, neuroactive ligand-receptor interactions for neural maturation, and ATP-binding cassette (ABC) transporters for detoxification. Adult maturation involves enhanced membrane dynamics, improved energy metabolism, and increased antioxidant defenses to support reproductive capacity and environmental resilience. Reproductive adults prioritize lipid metabolism and vitellogenin synthesis to fuel embryonic development. Crucially, host adaptation emerges as a critical driver of this stage-specific regulation. Additionally, multi-omics integration highlights a developmental transition from transcriptional dominance in early stages to post-transcriptional regulation in later phases. These insights provide a systems-level understanding of the physiological orchestration enabling this parasite's ecological success, while identifying valuable molecular targets for formulating more effective strategies to control this formidable pest.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal changes in prevalence of Sodalis glossinidius, Spiroplasma spp. and trypanosome species in wild Glossina tachinoides from Sora-Mboum animal African trypanosomiasis focus in northern Cameroon. 喀麦隆北部Sora-Mboum动物非洲锥虫病疫源地野生锥虫中舌索虫、螺旋体和锥虫种群流行率的时空变化

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-07 DOI: 10.1111/1744-7917.70160

Steve Feudjio Soffack, Tito T Melachio Tanekou, Hugues C Nana Djeunga, Oumarou Farikou, Ginette I Kame Ngasse, Mureille C Tchami Mbagnia, Murielle Wondji, Charles S Wondji, Hager Khalil, Adly M M Abd-Alla, Anne Geiger, Gustave Simo, Flobert Njiokou

{"title":"Spatiotemporal changes in prevalence of Sodalis glossinidius, Spiroplasma spp. and trypanosome species in wild Glossina tachinoides from Sora-Mboum animal African trypanosomiasis focus in northern Cameroon.","authors":"Steve Feudjio Soffack, Tito T Melachio Tanekou, Hugues C Nana Djeunga, Oumarou Farikou, Ginette I Kame Ngasse, Mureille C Tchami Mbagnia, Murielle Wondji, Charles S Wondji, Hager Khalil, Adly M M Abd-Alla, Anne Geiger, Gustave Simo, Flobert Njiokou","doi":"10.1111/1744-7917.70160","DOIUrl":"https://doi.org/10.1111/1744-7917.70160","url":null,"abstract":"Sterile Insect Technique (SIT) has proven effective to reduce tsetse population density in large infected areas where animal African trypanosomosis (AAT) and human African trypanosomiasis (HAT) elimination was difficult to achieve. However, the decrease in mass production of insectary-reared tsetse and the limited but incomplete knowledge on symbiont-trypanosome interaction over time, impede large-scale use of SIT. We investigated the spatiotemporal changes in symbiont prevalence and symbiont-trypanosome interactions in wild tsetse of Sora-Mboum AAT focus in northern Cameroon, collected in 2019 and 2020, to provide insights into the mass production of refractory tsetse. Spiroplasma spp., Sodalis glossinidius and trypanosomes were screened with PCR. G. tachinoides was the most abundant Glossina species found in Sora-Mboum focus. Symbiont prevalences in G. tachinoides were higher in 2019 compared to 2020, from 67.6% to 53.5% for Spiroplasma spp. and from 28.8% to 8.1% for S. glossinidius. These symbionts were also found at higher prevalence in flies from Mouhoun HAT focus in Burkina Faso. Four trypanosome taxa (Trypanosoma congolense forest type, T. congolense savannah type, T. brucei s.l., and T. vivax) were found in Sora-Mboum focus and Mouhoun focus, though at lower prevalence in Mouhoun. The presence of Spiroplasma spp. in adult tsetse was negatively associated with that of trypanosomes. Our study highlights the potential of Spiroplasma spp. as a good paratransgenesis candidate to enhance SIT application. This symbiont is naturally found in high proportions of tsetse and could prevent factory flies from acquiring and transmitting trypanosomes during their lifespan when released for population density control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the olive fly (Bactrocera oleae) microbiome across diverse geographic regions of Morocco. 摩洛哥不同地理区域橄榄蝇（油橄榄小实蝇）微生物组的特征。

IF 3 1区农林科学

Insect Science Pub Date : 2025-09-05 DOI: 10.1111/1744-7917.70126

Youssef El Yamlahi, Imane Remmal, Amal Maurady, Mohammed R Britel, Abdelmonaim Homrani Bakali, Naima Bel Mokhtar, Ioannis Galiatsatos, Panagiota Stathopoulou, George Tsiamis

{"title":"Characterization of the olive fly (Bactrocera oleae) microbiome across diverse geographic regions of Morocco.","authors":"Youssef El Yamlahi, Imane Remmal, Amal Maurady, Mohammed R Britel, Abdelmonaim Homrani Bakali, Naima Bel Mokhtar, Ioannis Galiatsatos, Panagiota Stathopoulou, George Tsiamis","doi":"10.1111/1744-7917.70126","DOIUrl":"https://doi.org/10.1111/1744-7917.70126","url":null,"abstract":"The olive fruit fly (Bactrocera oleae) is a significant pest threatening olive production worldwide. Bactrocera oleae relies on symbiotic bacteria for nutrition, development, and adaptation to its environment. Among these, Candidatus Erwinia dacicola is the most dominant symbiont and plays a key role in the fly's physiology and ecological adaptation. Understanding the dynamics between B. oleae, Ca. E. dacicola, and other components of the B. oleae microbiome is essential for developing effective targeted area-wide pest management strategies. This study aims to leverage full 16S rRNA gene sequencing to enhance the characterization of microbiome diversity in wild B. oleae populations from different regions in Morocco: Ouezzane, Rabat, Tanger, Errachidia, and Beni-Mellal. The results revealed distinct microbiome compositions influenced by geographic locations, with Candidatus Erwinia dacicola as the dominant symbiont, followed by Erwinia persicina as a secondary contributor. Other bacterial taxa, including Asaia bogorensis, were also identified, highlighting the functional diversity within the olive fly microbiome. These findings provide insights into the microbial ecology of B. oleae, contributing to the development and enhancement of sustainable pest control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0