Insect Science最新文献

Identification of mosquito olfactory receptors capable of detecting nitro compounds. 蚊子嗅觉受体对硝基化合物的识别。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-08 DOI: 10.1111/1744-7917.70041

Yuki Kodama, Atsufumi Ozoe, Michiru Hashimoto, Tokiro Ishikawa, Yasuhiko Takahashi, Sachiko Kitamoto

{"title":"Identification of mosquito olfactory receptors capable of detecting nitro compounds.","authors":"Yuki Kodama, Atsufumi Ozoe, Michiru Hashimoto, Tokiro Ishikawa, Yasuhiko Takahashi, Sachiko Kitamoto","doi":"10.1111/1744-7917.70041","DOIUrl":"https://doi.org/10.1111/1744-7917.70041","url":null,"abstract":"Insects possess an advanced olfactory system capable of detecting a wide range of odors through seven-transmembrane olfactory receptors (ORs). These ORs form heteromeric complexes with olfactory receptor co-receptor, Orco, and upon binding to specific ligands, they trigger the intracellular influx of ions such as sodium and calcium. Identifying ORs that respond to chemical molecules released from explosives holds significant importance for the development of biosensors for security and humanitarian purposes. In this study, screening of 196 mosquito ORs in HEK293FT cells for intracellular calcium flux on nitro compound administrations identified ORs as sensors for 2,4-dinitrotoluene, 2-nitroaniline, 2,3-dinitrotoluene, 2,6-dinitrotoluene, and 4-amino-2,6-dinitrotoluene. The different odor response profiles exhibited by naturally occurring polymorphisms or indels in the single OR gene that we had cloned were also explored. Sequence comparisons on these natural genetic variations and heterologous expression of each variant resulted in the identification of the amino acid positions involved critically in the gain and loss of odor sensitivity. Furthermore, we found that various combinations of the identified positions and different amino acid residues artificially evolve the OR with a higher sensitivity to nitro compounds. Our findings pave the way for the development of high-performance explosive detection biosensors, significantly contributing to technological advancements in landmine clearance and other areas. Additionally, our established screening system suggests the potential for identifying insect ORs that could serve as elements for various biosensors beyond explosive detection.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Spiroplasma infection on the mating behavior of Glossina fuscipes fuscipes. 螺旋体感染对fuscipes交配行为的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-08 DOI: 10.1111/1744-7917.70042

Giulia Fiorenza, Riccardo Piccinno, Daniel J Bruzzese, Francesca Scolari, Gloria Milanesi, Claudio Casali, Ludvik M Gomulski, Francesco Lescai, Federico Forneris, Giuliano Gasperi, Kiswend-Sida M Dera, Chantel de Beer, Adly M M Abd-Alla, Serap Aksoy, Anna R Malacrida

{"title":"Effect of Spiroplasma infection on the mating behavior of Glossina fuscipes fuscipes.","authors":"Giulia Fiorenza, Riccardo Piccinno, Daniel J Bruzzese, Francesca Scolari, Gloria Milanesi, Claudio Casali, Ludvik M Gomulski, Francesco Lescai, Federico Forneris, Giuliano Gasperi, Kiswend-Sida M Dera, Chantel de Beer, Adly M M Abd-Alla, Serap Aksoy, Anna R Malacrida","doi":"10.1111/1744-7917.70042","DOIUrl":"https://doi.org/10.1111/1744-7917.70042","url":null,"abstract":"Tsetse flies are insects of significant public health and zoonotic importance as they are the main vectors of African trypanosomes. To date, an effective vaccine is unavailable and efforts to limit the spread of the disease primarily rely on controlling the tsetse populations. The discovery of Spiroplasma (class Mollicutes) in Glossina fuscipes fuscipes (Gff) (palpalis subgroup), offers promising insights into its potential use as a biological control agent to hinder trypanosomes infection in tsetse flies. Indeed, a negative correlation between Spiroplasma and trypanosome co-infection has been observed. Using a laboratory strain of Gff, we provide fundamental biological insights into the effects of Spiroplasma infection on the mating behavior of the fly. We found a sex-biased Spiroplasma infection, with males exhibiting a higher infection rate. Mass mating experiments revealed a higher mating propensity in Spiroplasma-infected flies. Additionally, the presence of Spiroplasma influenced premating isolation, leading to nonrandom mating patterns that favored the pairing of individuals with matching infection statuses. Moreover, we present evidence of Spiroplasma vertical paternal transmission. By analyzing female reproductive tissues at 2 and 24 h postmating, we confirmed that an infected male can transfer Spiroplasma to the female via the spermatophore, which can subsequently migrate to the spermathecae. This study provides foundational insights into the role of Spiroplasma in tsetse fly mating behavior and provides supporting evidence for vertical transmission from infected males.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characteristics of pheromone binding proteins from Mythimna separata. 青菜费洛蒙结合蛋白的功能特性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-08 DOI: 10.1111/1744-7917.70029

Xiangqian Chang, Shu Zhang, Zuoqian Wang, Xiaolin Yang, Liang Lv, Manqun Wang

{"title":"Functional characteristics of pheromone binding proteins from Mythimna separata.","authors":"Xiangqian Chang, Shu Zhang, Zuoqian Wang, Xiaolin Yang, Liang Lv, Manqun Wang","doi":"10.1111/1744-7917.70029","DOIUrl":"https://doi.org/10.1111/1744-7917.70029","url":null,"abstract":"Olfactory plays an important role in insect behaviors. Pheromone binding proteins (PBPs) are thought to play a certain role in the transport of pheromone molecules in the olfactory recognition process for courtship and mating. Mythimna separata is one of the most serious cereal pests in Asia. The sexual pheromone components of M. separata were clarified; however, to date, little evidence in vivo or in vitro has disclosed the binding properties of PBPs toward the pheromone components of M. separata. To address this research gap, the functional characterization of PBPs in M. separata, spectroscopic investigations were conducted by using recombinant MsepPBPs. Subsequently, MsepPBP1 and MsepPBP3 were selected for RNA interference to assess changes in behavioral responses of male mutants toward normal females. Fluorescence displacement binding assays, combined with fluorescence quenching assays, revealed that MsepPBP3, among the 3 MsepPBPs, exhibited the strongest affinity for Z11-16:Ald, the primary component of sex pheromone in M. separata. Static quenching was observed only between MsepPBP1 and Z9-16:Ald, as well as between MsepPBP3 and Z11-16:Ald or Z9-16:Ald. Transcript levels of MsepPBP1 or MsepPBP3 of male adults were significantly reduced compared to the control when injected with dsMsepPBPs. Both dsPBP1- and dsPBP3-treated males displayed a notable decrease in successful calling behaviors, with this reduction being more pronounced in dsMsepPBP3 injected groups than in dsMsepPBP1 injected groups. These experiments indicated the specificity of MsepPBP1 and MsepPBP3, with both contributing to the sensitivity of female detection. MsepPBP3 appeared to be a key protein for recognizing the sex pheromones of M. separata.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic engineering for SIT application: a fruit fly-focused review. 基因工程在果蝇中的应用综述。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-07 DOI: 10.1111/1744-7917.70038

Serafima Davydova, Danheng Yu, Angela Meccariello

引用次数: 0

The inverse latitudinal diversity gradient and drivers in keratinophagous trogid beetles (Coleoptera: Trogidae). 食角蚁科蚁甲的纬向多样性梯度及其驱动因素。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-02 DOI: 10.1111/1744-7917.70035

Lu-Lu Li, Yuan-Yuan Lu, Xin Chen, Yi-Jie Tong, Xuan Zhou, Ning Liu, David Král, Ming Bai

引用次数: 0

Transglutaminase (TGase) regulates encapsulation response of the Oriental Armyworm, Mythimna separata (Lepidoptera: Noctuidae). 谷氨酰胺转胺酶（TGase）调控东方粘虫（Mythimna separata）囊化反应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-02 DOI: 10.1111/1744-7917.70039

Ying Zhu, Yuting Mao, Seiichi Furukawa

{"title":"Transglutaminase (TGase) regulates encapsulation response of the Oriental Armyworm, Mythimna separata (Lepidoptera: Noctuidae).","authors":"Ying Zhu, Yuting Mao, Seiichi Furukawa","doi":"10.1111/1744-7917.70039","DOIUrl":"https://doi.org/10.1111/1744-7917.70039","url":null,"abstract":"Transglutaminase (TGase) is a Ca2+-dependent acyltransferase that catalyzes protein cross-linking. We previously reported that the TGases of Mythimna separata, that is, MysTGase1 and MysTGase2, contribute to insect immune responses against microbial invaders. In this study, we aimed to elucidate the involvement of MysTGases in the encapsulation immune responses that target large invaders such as parasitoid eggs and nematodes. The expression levels of mystgases in hemocytes forming capsules around beads and nematodes were significantly higher than those in circulating hemocytes and increased in a size-dependent manner with increasing bead size. The expression of both mystgases was detected in plasmatocytes and granulocytes, which are the capsule components. RNA interference targeting mystgase1 or mystgase2 led to a significant decrease in the encapsulation rate of the beads, whereas treatment with recombinant MysTGases had the opposite effect. Furthermore, the knockdown of mystgase2 inhibited plasmatocyte spreading, whereas mystgase1 did not. This study highlights the potential significance of TGase in the encapsulation process and presents a notable molecular discovery of the encapsulation mechanism. This study also provides a scientific foundation for future research on insect immunity and may contribute to the development of innovative pest control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bombyx mori Cdc25 is involved in regulating the G2/M phase of the silkworm cell cycle. 家蚕Cdc25参与调节家蚕细胞周期的G2/M期。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-02 DOI: 10.1111/1744-7917.70037

Xi Yang, Yi Wei, Xiaolin Zhou, Cheng Lu, Peng Chen, Minhui Pan

{"title":"Bombyx mori Cdc25 is involved in regulating the G2/M phase of the silkworm cell cycle.","authors":"Xi Yang, Yi Wei, Xiaolin Zhou, Cheng Lu, Peng Chen, Minhui Pan","doi":"10.1111/1744-7917.70037","DOIUrl":"https://doi.org/10.1111/1744-7917.70037","url":null,"abstract":"Endoreplication plays a crucial role in the morphogenesis of various organs. The silk gland of Bombyx mori, which produces silk after maturation, engages in a unique cell cycle transition, mitosis-to-endoreplication, in the early stages of formation. This plays an essential role in the secretion of silk protein from silk gland cells. In this study, we identified and analyzed the expression characteristics of BmCdc25. The functional domain of BmCdc25 is conserved across species. BmCdc25 is mainly expressed in the G2/M phase of the cell cycle, and is expressed at low levels in the silk gland during active endoreplication. Overexpression of BmCdc25 enhanced cell proliferation, while inhibition of BmCdc25 caused cells to accumulate in the G2/M phase, thereby inhibiting cell proliferation. Additionally, BmCdc25 overexpression prevented DNA replication during silk gland cell endoreplication, while BmCdc25 inhibition had the opposite effect. BmZFP67 was confirmed to regulate BmCdc25 transcription by chromatin immunoprecipitation and electrophoretic mobility shift assays, while aberrant cytokinesis caused by knockout of BmZFP67 was shown to involve BmCdc25. Our findings expand the regulatory network of BmZFP67 from the perspective of transcriptional regulation and protein interaction and provide insight into the roles of BmCdc25 in the silkworm cell cycle.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing of LIM homeodomain transcription factor 1 alpha (Lmx1a) gene caused larval molting failure and adult reproductive deficiency in Henosepilachna vigintioctopunctata. LIM同源结构域转录因子1 α (Lmx1a)基因的沉默导致了章鱼Henosepilachna viintioctopunctata幼虫蜕皮失败和成虫繁殖不足。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-02 DOI: 10.1111/1744-7917.70033

Junna Liu, Zhaoyang Li, Zexin Zhong, Jianxing Li, Qi Chen, Ran Gao, Xuguo Zhou, Youjun Zhang, Chunxiao Yang, Huipeng Pan

{"title":"Silencing of LIM homeodomain transcription factor 1 alpha (Lmx1a) gene caused larval molting failure and adult reproductive deficiency in Henosepilachna vigintioctopunctata.","authors":"Junna Liu, Zhaoyang Li, Zexin Zhong, Jianxing Li, Qi Chen, Ran Gao, Xuguo Zhou, Youjun Zhang, Chunxiao Yang, Huipeng Pan","doi":"10.1111/1744-7917.70033","DOIUrl":"https://doi.org/10.1111/1744-7917.70033","url":null,"abstract":"LIM-HD (homeodomain) protein is essential for the specific differentiation of various cells and tissues due to its conserved HD. Within the LIM subclass, the LIM-HD transcription factor 1 alpha (Lmx1a) remains poorly characterized in insects. This study investigates the expression patterns of HvLmx1a in the 28-spotted lady beetle, Henosepilachna vigintioctopunctata, at both developmental stage and tissue levels. Furthermore, RNA interference (RNAi)-mediated knockdown of HvLmx1a resulted in increased mortality during the early larval stage. Injection of dsHvLmx1a at the beginning of the 4th instar caused a reduction in 20E titer, disrupted normal molting and pupation processes, and led to the emergence of larval-pupal abnormalities. Notably, HvLmx1a expression was significantly down-regulated on d 2 and 4 post-injection, which coincided with a marked inhibition of HvCHS1 and genes associated with 20E and bursicon signaling pathways. Additionally, silencing HvLmx1a induced qualitative changes in male testes and female ovaries, resulting in infertility and increased mortality. Ultimately, these findings suggest that HvLmx1a influences ovarian morphology and development through lipid metabolism. In conclusion, this study provides the first evidence of the diverse physiological roles of Lmx1a in H. vigintioctopunctata and highlight its potential as a target for RNAi-based biological control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleg coordination in free-walking bug Erthesina fullo (Hemiptera: Pentatomidae). 自由行走虫 Erthesina fullo（半翅目：五蠹科）的腿间协调。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-09 DOI: 10.1111/1744-7917.13412

Qingfei Han, Yuyu Chen, Huan Shen, Wei Wang, Xuefei Liu, Shikun Wen, Qian Qi, Zhendong Dai, Zhiwei Yu, Stanislav N Gorb, Aihong Ji

{"title":"Interleg coordination in free-walking bug Erthesina fullo (Hemiptera: Pentatomidae).","authors":"Qingfei Han, Yuyu Chen, Huan Shen, Wei Wang, Xuefei Liu, Shikun Wen, Qian Qi, Zhendong Dai, Zhiwei Yu, Stanislav N Gorb, Aihong Ji","doi":"10.1111/1744-7917.13412","DOIUrl":"10.1111/1744-7917.13412","url":null,"abstract":"Insects can adapt their walking patterns to complex and varied environments and retain the ability to walk even after significant changes in their physical attributes, such as amputation. Although the interleg coordination of intact insects has been widely described in previous studies, the adaptive walking patterns in free-walking insects with amputation of 1 or more legs are still unclear. The pentatomid bug Erthesina fullo exhibits a tripod gait, when walking freely on horizontal substrates, like many other insects. In this study, amputations were performed on this species to investigate changes in interleg coordination. The walking parameters were analyzed, such as the locations of touchdown and liftoff, cycle period, walking speed, and head displacement of intact and amputated insects. The results show that E. fullo displays adaptive interleg coordination in response to amputations. With 1 amputated leg, bugs changed to a 3-unit gait, whereas with 2 amputated legs they employed a wave gait. These data are helpful in exploring the motion mode control in walking insects and provide the theoretical basis for the gait control strategy of robots, when leg failure occurs.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"649-661"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foraging trail traffic rules: a new study method of trajectories of the harvester ants. 觅食路径交通规则：收割蚁轨迹的新研究方法。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-03 DOI: 10.1111/1744-7917.13411

Abderrahmane El Boukhrissi, Ahmed Taheri, Nard Bennas, Abdelkhalek Belkhiri, Bilal El Ajjouri, Joaquín L Reyes-López

{"title":"Foraging trail traffic rules: a new study method of trajectories of the harvester ants.","authors":"Abderrahmane El Boukhrissi, Ahmed Taheri, Nard Bennas, Abdelkhalek Belkhiri, Bilal El Ajjouri, Joaquín L Reyes-López","doi":"10.1111/1744-7917.13411","DOIUrl":"10.1111/1744-7917.13411","url":null,"abstract":"Harvester ants are one of the most extensively studied groups of ants, especially the group foraging ants, Messor barbarus (Linnaeus, 1767), which construct long-lasting trunk trails. Limited laboratory investigations have delved into head-on encounters along foraging trails involving workers moving in opposing directions, with fewer corresponding studies conducted in the natural environment. To address this gap, we devised an in-field experimental design to induce lane segregation on the foraging trunk trail of M. barbarus. Using an image-based tracking method, we analyzed the foraging behavior of this species to assess the costs associated with head-on encounters and to figure out the natural coexistence of outgoing and returning workers on a bidirectional route. Our results consistently reveal heightened straightness and speed in unidirectional test lanes, accompanied by an elevated foraging rate compared to bidirectional lanes. This suggests a potential impact of head-on collisions on foraging behavior, especially on foraging efficiency. Additionally, Kinematic analysis revealed distinct movement patterns between outbound and inbound flows, particularly low speed and sinuous trajectories of inbounding unladen workers. The study of encounter rates in two traffic systems hints at the plausible utilization of individual memory by workers within trails, underscoring the pivotal role of encounters in information exchange and load transfer.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"687-700"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0