Insect Science最新文献

Epigenetic remodeling of DNA methylation is associated with pupal diapause regulation in Helicoverpa armigera. DNA甲基化的表观遗传重塑与棉铃虫蛹滞育调控有关。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-18 DOI: 10.1111/1744-7917.70379

Yan Li, Shunchao Xia, Dingxue Cai, Guy Smagghe, Zhaolang Teng, Jianjun Guo, Haiyin Li

{"title":"Epigenetic remodeling of DNA methylation is associated with pupal diapause regulation in Helicoverpa armigera.","authors":"Yan Li, Shunchao Xia, Dingxue Cai, Guy Smagghe, Zhaolang Teng, Jianjun Guo, Haiyin Li","doi":"10.1111/1744-7917.70379","DOIUrl":"https://doi.org/10.1111/1744-7917.70379","url":null,"abstract":"Diapause is a key seasonal adaptation that enables insects to survive predictable environmental adversity, yet the molecular mechanisms linking environmental cues to stable developmental decisions remain incompletely understood. We hypothesized that DNA methylation contributes to the epigenetic regulation of pupal diapause in the cotton bollworm Helicoverpa armigera. To test this hypothesis, we combined gene expression analyses, pharmacological manipulation, whole-genome bisulfite sequencing (WGBS), transcriptome profiling, and functional validation of candidate genes. Diapause-destined pupae exhibited reduced expression of DNA methylation-associated factors, accompanied by lower genomic 5-methylcytosine levels. Pharmacological inhibition of DNA methylation delayed pupal development and induced a reversible diapause-like arrest in a subset of nondiapause pupae. Integrated methylome and transcriptome analyses revealed extensive stage-dependent remodeling of DNA methylation and gene expression, identifying pathways associated with developmental regulation, energy metabolism, and amino acid metabolism. Among the methylation-associated candidates, BCAT1 was consistently upregulated during diapause, and pharmacological inhibition of BCAT1 promoted diapause termination, supporting its involvement in diapause regulation. Taken together, our findings identify DNA methylation as an important epigenetic component associated with diapause programming and provide a framework for understanding how seasonal environmental signals shape developmental plasticity in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Form, function, and evolution of worker-worker social carrying in ants. 蚂蚁工蚁社会搬运的形式、功能与演化。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-14 DOI: 10.1111/1744-7917.70377

Arik Dorfman, Shahar Dubiner, Inon Scharf

{"title":"Form, function, and evolution of worker-worker social carrying in ants.","authors":"Arik Dorfman, Shahar Dubiner, Inon Scharf","doi":"10.1111/1744-7917.70377","DOIUrl":"https://doi.org/10.1111/1744-7917.70377","url":null,"abstract":"Social insects provide valuable insights into how coordinated group behavior emerges from simple individual rules. In ants, recruitment strategies are diverse, yet some remain poorly understood from a comparative evolutionary perspective. Social carrying, in which one individual physically transports another, is a widespread recruitment strategy associated with several functions, most commonly nest relocation. Despite extensive descriptive work, its evolutionary origins, variation, and ecological correlates remain unclear. We compiled literature data on worker-worker social carrying in ant species from seven subfamilies and integrated these data with phylogenetic analyses to address these questions. Using ancestral state estimation, we reconstructed the evolution of carrying posture and stereotypicality (the rigidity of the behavior). We also tested whether colony structure affects navigation ability after transport. Contrary to previous suggestions, we found that the ancestral form of social carrying was a stereotypical, coordinated interaction that later evolved into less stereotypical and sometimes aggressive forms. Carrying posture was largely subfamily specific. Social carrying occurred in species with colonies ranging from a few dozen to several million individuals, indicating that its use is not constrained by colony size. In addition, some polydomous species were able to navigate after being carried, whereas no monodomous species were. This study provides a broad evolutionary perspective on worker-worker social carrying and highlights new directions for research on recruitment in social insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Divergent migratory strategies along the East Asian insect flyway: A trade-off between flight power and atmospheric dependence. 东亚昆虫迁徙路线上的不同迁徙策略：飞行能力和对大气依赖之间的权衡。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-13 DOI: 10.1111/1744-7917.70376

Boya Gao, Ye-Chen Wang, Jun-Sheng Zhu, Yi-Yang Zhang, Chen Zhou, Bei-Bei Wang, Don R Reynolds, Jason W Chapman, Yan Wu, Gao Hu

{"title":"Divergent migratory strategies along the East Asian insect flyway: A trade-off between flight power and atmospheric dependence.","authors":"Boya Gao, Ye-Chen Wang, Jun-Sheng Zhu, Yi-Yang Zhang, Chen Zhou, Bei-Bei Wang, Don R Reynolds, Jason W Chapman, Yan Wu, Gao Hu","doi":"10.1111/1744-7917.70376","DOIUrl":"https://doi.org/10.1111/1744-7917.70376","url":null,"abstract":"Mass migration within the East Asian insect flyway is a globally significant ecological phenomenon, yet how insects with different flight capabilities utilize divergent strategies to exploit the same wind system remains a critical puzzle. We hypothesize that these strategies arise from a fundamental trade-off between active trajectory control and strategic atmospheric exploitation. To test this, we integrated long-term (11-year) field observations with atmospheric trajectory modeling to quantitatively assess the migratory performance of two species with disparate flight capacities: the oriental armyworm moth (Mythimna separata), a relatively powerful flier, and the brown planthopper (Nilaparvata lugens), a weak flier. Our simulations reveal that the strong-flying M. separata, which initiates its northward migration from mid-May, actively influences its trajectory, leveraging its self-powered flight to overcome slower, less directionally stable winds. In contrast, the weaker-flying N. lugens, which migrates about a month later, synchronizes its migration with a period of faster, more stable wind currents, allowing it to compensate for its limited flight power. These findings provide a mechanistic demonstration of how a trade-off between self-propelled power and atmospheric selectivity shapes migratory success. Ultimately, this study establishes that flight capacity is a key determinant of how migratory insects interact with their atmospheric environment, which in turn impacts their contrasting life histories within a shared flyway.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing bacterial symbionts of beetles for the valorization of lignin into value-added byproducts: Recent advances and future perspectives. 利用甲虫的细菌共生体将木质素转化为增值副产品：最新进展和未来展望。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-09 DOI: 10.1111/1744-7917.70378

Bintou Doumbia, Shijie Luo, Sixu Yang, Chengwei Wang, Yuxin Jia, Alexander V Kuprin, Alei Geng, Daochen Zhu, Jianzhong Sun, Bonaventure Chidi Ezenwanne, Rongrong Xie, Mudasir A Dar

{"title":"Harnessing bacterial symbionts of beetles for the valorization of lignin into value-added byproducts: Recent advances and future perspectives.","authors":"Bintou Doumbia, Shijie Luo, Sixu Yang, Chengwei Wang, Yuxin Jia, Alexander V Kuprin, Alei Geng, Daochen Zhu, Jianzhong Sun, Bonaventure Chidi Ezenwanne, Rongrong Xie, Mudasir A Dar","doi":"10.1111/1744-7917.70378","DOIUrl":"https://doi.org/10.1111/1744-7917.70378","url":null,"abstract":"Lignin is the most abundant renewable aromatic heteropolymer in nature and is highly recalcitrant due to its complex structure. The gut systems of beetles function as an evolutionarily optimized microreactor wherein mechanical pretreatment, physicochemical gradients, and selectively maintained microbial consortia act synergistically to transform, mineralize, and metabolize lignin as an energy source. Beetle-associated bacterial symbionts represent a promising yet underexploited resource for lignin valorization into value-added products. This review provides emerging evidence of lignin degradation by beetles in association with their gut symbionts and indicates the diversity of gut bacteria involved in lignin depolymerization. We further discuss the mechanisms by which beetles and their gut bacteria metabolize lignin, the lignin-transforming capacities of the bacterial symbionts and their potential application in the valorization of waste lignin into added-value byproducts. Finally, we assess the emerging research trends on the bioprospection of lignin-degrading bacteria from underexplored beetle species. Despite significant advantages, several key challenges remain unresolved, including difficulties in isolation and culturing of key gut bacteria in vitro, limited biochemical validation of candidate enzymes and the unresolved linking of specific genes or taxa to in situ lignin transformation. Future studies should integrate discovery-driven microbial ecology, multi-omics, predictive modeling and precision genome engineering to convert the complexity of beetle gut bacterial communities into a practical blueprint for scalable lignin valorization, and the biomanufacturing of high-value products from lignin-based waste.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmSNX20 restricts Bombyx mori nucleopolyhedrovirus infection and is associated with autophagy-mediated antiviral immunity. BmSNX20限制家蚕核多角体病毒感染，并与自噬介导的抗病毒免疫有关。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-09 DOI: 10.1111/1744-7917.70368

Jian He, Guangyu Ma, Tiandong Li, Liang Xu, Hanyang Wang, Doudou Hu, Jingchen Sun

{"title":"BmSNX20 restricts Bombyx mori nucleopolyhedrovirus infection and is associated with autophagy-mediated antiviral immunity.","authors":"Jian He, Guangyu Ma, Tiandong Li, Liang Xu, Hanyang Wang, Doudou Hu, Jingchen Sun","doi":"10.1111/1744-7917.70368","DOIUrl":"https://doi.org/10.1111/1744-7917.70368","url":null,"abstract":"Sorting nexins (SNXs) are phosphoinositide-binding proteins that play critical roles in membrane trafficking and have been implicated in antiviral immunity. However, the function of SNX20 in insect antiviral immunity remains poorly defined. In this study, we investigated the role of Bombyx mori SNX20 (BmSNX20) during infection with Bombyx mori nucleopolyhedrovirus (BmNPV). Phylogenetic and domain analyses revealed that SNX20 is evolutionarily conserved across insect species and contains a canonical phox homology (PX) domain. Expression profiling showed that BmSNX20 is highly expressed in the midgut and fat body and is significantly upregulated upon BmNPV infection in both larvae and BmN cells. Functional analyses demonstrated that BmSNX20 knockdown significantly enhanced viral replication, whereas its overexpression suppressed viral gene expression, reduced viral titers, and improved host survival, indicating a clear antiviral role. Mechanistically, BmSNX20 overexpression activated multiple immune pathways, including Imd, JAK/STAT signaling, RNA interference, STING and notably promoted autophagy, as indicated by upregulated expression of ATG5 and ATG8, enhanced MDC fluorescence, increased conversion of ATG8 to its lipidated form ATG8-PE, and strong colocalization between BmSNX20 and ATG8. These demonstrate that BmSNX20 overexpression triggers an autophagic response, which may contribute to its antiviral activity, in addition to the activation of multiple other immune pathways. In conclusion, this study identified BmSNX20 as a novel antiviral factor in silkworms and highlighted its role in linking membrane trafficking to autophagy and innate immunity. These findings expand our understanding of SNX protein functions in insect antiviral defense and provide novel insights into host-virus interactions in invertebrates.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri. 核受体PcHR96h调控柑橘全爪虱抗螺双氯芬相关的ABC转运蛋白。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-09 DOI: 10.1111/1744-7917.70373

Sichen Li, Shijiang Yu, Qiqi Yang, Zehao Huang, Yang Li, Mengxia Wang, Meiyi Huang, Shijun Fu, Zhen Wu, Liu Liu, Qi Pan, Wei Zhang, Haoqiang Liu, Lin Cong, Chun Ran

引用次数: 0

Source tracking and transmission of antibiotic resistance genes mediated by core microbiota in black soldier fly larvae bioconversion of doxycycline-contaminated hen manure. 多西环素污染鸡粪中黑虻幼虫生物转化中核心菌群介导的抗生素耐药基因来源追踪及传播

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-07 DOI: 10.1111/1744-7917.70366

Jingyuan Chen, Weikang Deng, Junliang He, Shihua Niu, Sicheng Xing, Xindi Liao

{"title":"Source tracking and transmission of antibiotic resistance genes mediated by core microbiota in black soldier fly larvae bioconversion of doxycycline-contaminated hen manure.","authors":"Jingyuan Chen, Weikang Deng, Junliang He, Shihua Niu, Sicheng Xing, Xindi Liao","doi":"10.1111/1744-7917.70366","DOIUrl":"https://doi.org/10.1111/1744-7917.70366","url":null,"abstract":"Black soldier fly larvae (BSFL) bioconversion of doxycycline-contaminated hen manure raises concerns about antibiotic resistance gene (ARG) transmission. Although core microbiota in gut may participate in ARG dynamics, the relative contributions of microbiota from manure and baseline larval gut to the BSFL gut resistome remain unclear. Using metagenomics, amplicon sequencing, and cultivable bacteria analysis, we found that: (1) Providencia, Klebsiella, Enterococcus, and Escherichia-Shigella dominated the BSFL gut and served as primary ARG hosts; (2) Fecal filtrate intervention drastically altered gut microbiota and ARG profiles, suppressing Klebsiella (by 95.56%) and Escherichia-Shigella (to < 0.5%) while enriching Providencia (3.53 fold increase) and Enterococcus, with increased ARGs such as tet(59) and qnrD1. Long-read metagenomic analysis indicated that viable manure-borne bacteria, rather than cell-free fecal filtrate, delivered structurally intact ARG-MGE units as key carrier of mobile resistance cassettes; (3) Source tracking (FEAST) assigned the majority of ARBs (> 90%) and their ARGs in the BSFL gut to the baseline BSFL gut source, with manure-derived ARBs contributing 4.80%. Culture-based 16S rRNA gene homology provided evidence consistent with possible manure-to-gut transfer of selected Escherichia-Shigella and Enterococcus. The BSFL gut resistome was more strongly associated with the baseline BSFL microbiota than with manure, but manure still represented a detectable source of ARB and ARG introduction. FEAST source assignments should be interpreted as composition-based bioinformatic estimates rather than definitive proof of origin, due to lack of direct functional tracing. These findings have critical implications for assessing the ecological safety of BSFL-based waste valorization.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of dopaminergic neuronal clusters in governing division of labor in ants. 多巴胺能神经元簇在蚂蚁劳动分工中的作用。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-06-27 DOI: 10.1111/1744-7917.70087

Wenjiang Zhong, Nianxia Xie, Guo Ding, Jie Zhao, Pei Zhang, Qiye Li, Hao Ran, Guojie Zhang, Weiwei Liu

{"title":"The role of dopaminergic neuronal clusters in governing division of labor in ants.","authors":"Wenjiang Zhong, Nianxia Xie, Guo Ding, Jie Zhao, Pei Zhang, Qiye Li, Hao Ran, Guojie Zhang, Weiwei Liu","doi":"10.1111/1744-7917.70087","DOIUrl":"10.1111/1744-7917.70087","url":null,"abstract":"Reproductive division of labor is one of the most prominent features of social insects. Yet, the neural mechanisms that govern this division and the associated behavioral differentiation among castes remain obscure. In this study, we systematically characterized the anatomical features of dopamine neurons in the brain of Monomorium pharaonis and compared the cell number and spatial distribution of these neurons across castes. We identified 17 anatomically distinct clusters of dopamine neurons in the ant brain, with cell numbers varying from ∼322 to ∼431 across castes. The major dopamine clusters are located in brain regions analogous to those in flies, with 2 clusters, PAL and PPL1 exhibiting significantly higher cell numbers in ants than in flies. Notably, 4 clusters, DAM, D1, DPL, and PPL2, showed remarkable variation in cell numbers across castes. Using single-cell transcriptomics, we identified specific molecular markers for subdividing dopamine neurons. We validated the expression of multiple neuropeptide genes in specific dopamine clusters. In particular, we found that PPL2 cluster can be further divided into 2 subclusters, PPL2a and PPL2b, which are partially labeled by the peptide gene Nplp1. PPL2b neurons, characterized by larger cell bodies, and present only in unmated queens and males, are absent in mature queens and workers. These neurons are located adjacent to Nlg2-expressing lobular neurons, which are also absent in workers and may play a role in regulating mating behaviors. Our findings provide a foundation for further investigation into the neural mechanism underlying division of labor and caste-specific behaviors in ant.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2081-2096"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arthropod diversity dynamics in guava and cherimoya plantations affected by Solenopsis invicta invasion. 无尾螺线苋入侵对番石榴和番荔枝人工林节肢动物多样性的影响

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-04 DOI: 10.1111/1744-7917.70119

Yuling Liang, Yunbo Song, Jingxin Hong, Yangting Ou, Meng Chen, Jiarui Wu, Huimei Chen, Mingrong Liang, Yongyue Lu

{"title":"Arthropod diversity dynamics in guava and cherimoya plantations affected by Solenopsis invicta invasion.","authors":"Yuling Liang, Yunbo Song, Jingxin Hong, Yangting Ou, Meng Chen, Jiarui Wu, Huimei Chen, Mingrong Liang, Yongyue Lu","doi":"10.1111/1744-7917.70119","DOIUrl":"10.1111/1744-7917.70119","url":null,"abstract":"The red imported fire ant (Solenopsis invicta, RIFA) is one of the most ecologically disruptive invasive species, yet its impacts on arthropod biodiversity within subtropical orchard agroecosystems-particularly guava (Psidium guajava) and cherimoya (Annona cherimola) plantations-remain insufficiently quantified. This study investigated how RIFA invasion influences arthropod community composition, diversity and trophic group dynamics across these 2 plantation systems in southern China. Results indicated that RIFA invasions significantly reduced both order- and family-level richness and evenness. Arthropod order richness declined markedly in invaded areas, with only 8 orders detected in invaded cherimoya plots compared to 11 in non-invaded plots. At the family level, diversity decreased by 75% in guava plantations (from 8 to 2 families) and by 60% in cherimoya plantations (from 10 to 4 families). In invaded plots, RIFAs constituted over 80% of the total arthropod population. Hedges' g effect sizes further revealed substantial increases in overall arthropod abundance (+277.62%) and RIFA-specific abundance (+357.42%), coupled with significant declines in family richness (-16.29%) and non-RIFA arthropod abundance (-22.78%). Piecewise structural equation modeling indicated that RIFA exerted strong, negative impacts on detritivores and omnivores, while herbivores were less affected, indirectly suppressing predators by reducing their prey base. Collectively, these findings suggested that RIFA invasion drove pronounced taxonomic homogenization, functional simplification and trophic imbalance within agricultural landscapes. Effective, long-term monitoring and biodiversity-focused management strategies are urgently needed to mitigate the ecological degradation driven by RIFA invasions in these critical agroecosystems.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2189-2209"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ovary-targeted nucleic acid delivery system, OT-NADPS, efficiently mediates the generation of transgenic silkworms. 一种卵巢靶向核酸传递系统OT-NADPS有效介导转基因家蚕的产生。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-08-06 DOI: 10.1111/1744-7917.70132

Lan Hu, Zhan Wang, Pengchao Guo, Yuancheng Wang, Huawei Liu, Ping Zhao, Qingyou Xia

{"title":"An ovary-targeted nucleic acid delivery system, OT-NADPS, efficiently mediates the generation of transgenic silkworms.","authors":"Lan Hu, Zhan Wang, Pengchao Guo, Yuancheng Wang, Huawei Liu, Ping Zhao, Qingyou Xia","doi":"10.1111/1744-7917.70132","DOIUrl":"10.1111/1744-7917.70132","url":null,"abstract":"Transgenic technology is crucial for advancing various areas of biological research. In silkworms, the traditional transgenic method relies on embryo microinjection, which is a labor-intensive process with limited efficiency and high technical barriers. To overcome these challenges, we developed an Ovary-Targeted Nucleic Acid Delivery Peptide, OT-NADPS, based on the Receptor-Mediated Ovary Transduction of Cargo system (ReMOT) by innovative integration. OT-NADPS, which consists of a nucleic acid loading domain and an ovarian targeting peptide, demonstrates strong nucleic acid binding capacity and excellent binding stability. It can efficiently deliver nucleic acid to silkworm ovaries through pupae injection, thereby enabling the generation of transgenic silkworms. Using the OT-NADPS delivery system, transgenic silkworms were obtained with a success rate of 3.92%, comparable to the 2.0%-5.0% success rate range in traditional embryo microinjection. This novel method shows a lower technical threshold and a higher success rate, which is potentially applied to transgenic operations in multiple insect species.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1753-1768"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0