Insect Science最新文献

{"title":"Dextran sodium sulfate-induced colitis-like gut permeability and dysbiosis in honeybees.","authors":"Ya Li, Jieteng Chen, Xiaohuan Mu, Xiaofei Wang, Yan Liu, Hao Chen, Li Zheng, Yifan Zhai, Hao Zheng, Yiyuan Li","doi":"10.1111/1744-7917.13508","DOIUrl":"https://doi.org/10.1111/1744-7917.13508","url":null,"abstract":"<p><p>The gut microbiota has been linked to the pathophysiology of inflammatory bowel disease (IBD). Current animal models are limited in the generation of germ-free animals to evaluate the roles of gut bacteria in intestinal health. Here, we used the honeybee (Apis mellifera) as a model animal to develop colitis-like gut permeability induced by dextran sodium sulfate (DSS) treatment. We found that DSS could increase gut permeability and compromise the mucosal barrier in honeybees, resulting in decreased survival rates, midgut elongation, and intestinal edema. DSS treatment upregulated the expression of wnt-1 and rhomboid and downregulated the pathways of cytochrome P450 (CYP) and the peroxisome proliferator-activated receptor signaling. Moreover, DSS disrupted the structure and functional profiles of the gut microbiota. Our results showed that medications reduced mortality and moderated leaky gut syndrome in DSS-treated bees. Notably, 5-aminosalicylic acid (5-ASA) could attenuate gut permeability in honeybees through activating PPARγ signaling, which mimics the human. Honeybees offer a promising experimental model for investigating the etiology of intestinal diseases and disentangling the roles of gut symbionts in intestinal health.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cretaceous lacewing larvae with binocular vision demonstrate the convergent evolution of sophisticated simple eyes.","authors":"Carolin Haug, Florian Braig, Simon J Linhart, Derek E G Briggs, Roland R Melzer, Alejandro Caballero, Yanzhe Fu, Gideon T Haug, Marie K Hörnig, Joachim T Haug","doi":"10.1111/1744-7917.13509","DOIUrl":"https://doi.org/10.1111/1744-7917.13509","url":null,"abstract":"<p><p>Many insects and their relatives are renowned for sophisticated compound eyes, which are also preserved in the fossil record. Yet there are other types of eyes, notably the so-called stemmata of holometabolans, such as beetles, bees, and butterflies. Stemmata are not as effective as compound eyes, except in some predatory larvae. Here we report three lacewing larvae with large forward-directed stemmata from Cretaceous Kachin amber, Myanmar. The stemmata are large relative to those of other fossil lacewing larvae, comparable to the simple eyes of modern larvae capable of image formation. The head is very wide in one larva, representing a new type of morphology as demonstrated by a quantitative comparison of the head and stylets of over 400 fossil and extant lacewing larvae. The arrangement of the exceptionally large stemmata of the larvae reported here provides stereoscopic vision. These new specimens demonstrate the convergent evolution of highly developed simple eyes in at least two additional lineages of lacewings, showcasing the enormous diversity of lacewing larvae in the Cretaceous.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diapause hormone receptor affects larval growth and embryonic development in the multivoltine strain of Bombyx mori.","authors":"Wenhui Jing, Yun Gao, Yinghui Li, Yue Jin, Yanyan Zhou, Yusong Xu, Huabing Wang","doi":"10.1111/1744-7917.13507","DOIUrl":"https://doi.org/10.1111/1744-7917.13507","url":null,"abstract":"<p><p>Diapause is a programmed developmental arrest process in insects. Diapause can occur at various stages of insect development and is frequently restricted to a specific developmental stage within a single species. In Bombyx mori, embryonic diapause is elicited by the diapause hormone (DH) and DH receptor (DHR) in diapause strains. Nevertheless, the regulatory mechanism through which BmDHR functions as a G protein-coupled receptor (GPCR), to exert other physiological functions in nondiapause silkworms, remains unclear. In this study, we found that BmDHR had 7 alternative splice isoforms. A knockout experiment confirmed that BmDHR mediated the transduction of diapause signals. Interestingly, the loss of BmDHR caused partial precocious metamorphosis and an embryo-lethal phenotype in nondiapause silkworms. An assessment of global transcriptional patterns revealed that BmDHR knockout affected physiological responses induced by manifold cellular processes, including the Toll/immune deficiency (Imd), Wnt, insulin-like growth factor, Hedgehog and P38/mitogen-activated protein kinase (MAPK) signaling pathways. This study expands our knowledge of the physiological roles for DHR in insect growth and development.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MESR4 targets bam to mediate intestinal homeostasis and aging in adult flies.","authors":"Qingshuang Cai, Zixuan Wang, Yihua Xiao, Chuchu Zhang, Yulong Yang, Fanrui Kong, Yue Feng, Huimin Guo, Muhammad Abdul Rehman Saeed, Umar Ali, Weini Li, Shanming Ji","doi":"10.1111/1744-7917.13506","DOIUrl":"https://doi.org/10.1111/1744-7917.13506","url":null,"abstract":"<p><p>The Drosophila melanogaster (fruit fly) misexpression suppressor of Ras 4 (MESR4) gene encodes a potential transcription factor and plays critical roles in various biological processes, including embryonic development, lipid metabolism, eye-antennal development, and germline stem cell differentiation. However, whether it is involved in modulating intestinal homeostasis remains elusive. In this study, we provide compelling evidence demonstrating that MESR4 is a bona fide regulator in preventing age-onset intestinal leakage and dysbiosis in adult flies. Mechanistically, MESR4 is predominantly located at the nucleus of intestinal cells and controls the expression of bag-of-marbles (bam), thereby restricting the excessive activation of immune deficiency signaling during aging. The silencing of Relish (Rel), which encodes a key transcription factor of the immune deficiency signaling pathway, reverses the beneficial effects of MESR4 in mediating intestinal barrier function and fly lifespan. Collectively, our studies uncover an undescribed function of Drosophila MESR4 in the maintenance of intestinal homeostasis and overall organismal fitness.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agonistic interactions between Vespula vulgaris (Linnaeus) and Vespula germanica (Fabricius) (Hymenoptera: Vespidae) during foraging.","authors":"María Celeste Manattini, Mariana Lozada, Micaela Buteler","doi":"10.1111/1744-7917.13500","DOIUrl":"https://doi.org/10.1111/1744-7917.13500","url":null,"abstract":"<p><p>Foraging behavior is a key factor associated with the success of social insect invasions. Vespula wasps show complex behavioral patterns and social mechanisms associated with foraging, which are directly related to their invasive success in several countries worldwide. Vespula vulgaris (Linnaeus) and Vespula germanica (Fabricius) are invasive wasps, coexisting in Patagonia, showing temporal and dietary overlap. As generalist, opportunistic predators and scavengers with broad diets, these sympatric wasps share similar niches and foraging habits. We analyzed their foraging strategies and interaction, observing the behavior of each species in the presence of workers of the same species, and the other species at a given resource, directly and indirectly. Directly, from a continuous visual record, we observed and recorded the frequency of wasp's behavior at a feeder for 30 min. Indirectly, we conducted pairwise choice tests to compare wasp preference for treated and untreated feeders with visual and olfactory cues simulating the presence of wasps of the same or different species. We found consistent differences between species in aggressiveness. V. vulgaris showed a greater degree of intraspecific agonistic behavior than toward V. germanica, while V. germanica was less aggressive, and intraspecific and interspecific interactions were similar. Also, V. vulgaris preferred landing on baits without visual and olfactory cues simulating wasps presence, while V. germanica preferred baits with cues simulating its own species over baits with cues representing V. vulgaris. Our results suggest that V. germanica prioritize social facilitation as foraging strategy, while for V. vulgaris aggression is the predominant strategy.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristic and functional study of intersex, a gene involved in sexual development in Gryllus bimaculatus.","authors":"Jia-Xin Duan, Li-Dong Shi, Yun Bai, Mi Di, Ning Zhang, Gang-Qi Fang, Dong-Liang Li, Zhu-Qing He, Kai Li","doi":"10.1111/1744-7917.13511","DOIUrl":"https://doi.org/10.1111/1744-7917.13511","url":null,"abstract":"<p><p>As a gene required for sexual development, intersex (ix), functions in concert with the female-specific product of doublesex (dsx) at the end of the hierarchy to facilitate the sex-specific differentiation of sexually dimorphic characters in female Drosophila melanogaster. In the present study, we initially identified the ix homolog in Gryllus bimaculatus, with the detection of a single isoform expressed in both sexes. Phylogenetic analyses and multiple sequence alignment revealed that Gbix exhibited conservation. Here we employed RNA interference (RNAi) and clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9), respectively, to analyze the functions of Gbix. Our findings indicated that Gbix played a crucial role in the normal development of the embryo and nymph, as well as in the regulation of wing morphogenesis in both sexes. RNAi-mediated knockdown of Gbix resulted in poorly developed ovaries, whereas testicular development was not significantly affected. Nevertheless, CRISPR/Cas9-mediated knockout of Gbix resulted in internal genital defects in both sexes, which ultimately led to a reduction in reproductive capacity in female and male individuals. Our results provide insights into the pleiotropic functions of Gbix in embryogenesis and sexual development, while also advancing our comprehension of sex determination in a hemimetabolous insect species.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the cryptic Bemisia tabaci species complex: Global phylogenomic analysis reveals evolutionary relationships and biogeographic patterns.","authors":"Hualing Wang, Shilong Geng, Shusheng Liu, Zhongtao Li, Stephen Cameron, Teng Lei, Wei Xu, Qing Liu, Shuang Zuo, Christopher A Omongo, M N Maruthi, Habibu Mugerwa, Xiaowei Wang, Yinquan Liu, Jesús Navas-Castillo, Elvira Fiallo-Olivé, Kyeong-Yeoll Lee, Renate Krause-Sakate, Hélène Delatte, James Ng, Susan Seal, John Colvin","doi":"10.1111/1744-7917.13501","DOIUrl":"https://doi.org/10.1111/1744-7917.13501","url":null,"abstract":"<p><p>Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades, substantial genetic diversity along with strong phylogeographic associations. However, a comprehensive phylogenomic analysis across the entire complex has been lacking, we thus conducted phylogenomic analyses and explored biogeographic patterns using 680 single-copy nuclear genes (SCNs) obtained from whole-genome sequencing data of 58 globally sourced B. tabaci specimens. We constructed both concatenation and coalescent trees using 680 SCNs, which produced highly supported bootstrap values and nearly identical topologies for all major clades. When comparing these concatenation trees with those constructed using mitochondrial cytochrome oxidase I (mtCOI) and mitochondrial genome, we found conflicting phylogenetic relationships, with the later trees recovering fewer major clades. In a separate comparison between concatenation and coalescent trees, particularly those generated using IQ-TREE, they were found to delineate population relationships more effectively than RaxML. In contrast, coalescent phylogenies were proficient in elucidating geographical dispersal patterns and the reorganization of biological species. Furthermore, we provided a strict consensus tree that clearly defines relationships within most clades, laying a solid foundation for future research on the evolution and taxonomy of B. tabaci. Ancestral range estimates suggested that the ancestral region of the complex is likely situated in equatorial Africa, the Middle East, and Mediterranean regions. Subsequently, the expansion occurred into part of the Palearctic and further into the Nearctic, Neotropical, Indomalayan, and Australasian regions. These findings challenge both previous classifications and origin hypotheses, offering a notably more comprehensive understanding of the global distribution, evolutionary history, diversification, and biogeography of B. tabaci.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of swine manure mixed with circulating fluidized bed fly ash on black soldier fly (Diptera: Stratiomyidae) larvae and larval frass.","authors":"Jianwei Hao, Shuang Liu, Mengliang Wang, Wenfeng Hu, Jia Zhao","doi":"10.1111/1744-7917.13513","DOIUrl":"https://doi.org/10.1111/1744-7917.13513","url":null,"abstract":"<p><p>Black soldier fly larvae (BSFL) were reared on mixtures of swine manure and circulating fluidized bed fly ash (CFA) in different ratios. The aim was to evaluate the impacts of insoluble inorganic matter on BSFL and larval frass. The growth performance and nutrient composition of the BSFL were measured under different treatments. The intestinal microbiota structure, morphological characteristics, and total proteolytic activity of the gut were analyzed. The larval frass was tested for nutrients and analyzed using energy-dispersive spectroscopy and scanning electron micrographs. In particular, the surface areas of microparticles from the larval frass (diameter < 0.0074 mm) were measured using Brunauer-Emmett-Teller method. It was found that CFA addition prolonged larval development and reduced the maximum larval weights. The mean larval length, crude protein, and highest larval weight showed negative regression with an increase in the CFA ratio (P < 0.05). Morphological images indicated that physical clogging might be the main influencing factor on larval growth. Moreover, the microbial diversity and complexity in the larval gut increased with CFA addition, but CFA addition had little effect on the composition of dominant phyla or genera (P > 0.05). Finally, the nutrient composition revealed that the frass met the organic fertilizer standard when the CFA addition ratio was less than 7.5%. The optimal addition ratio was 5%, at which the larvae had a more stable and healthier gut environment, but there was less of an effect on larval growth and nutrient composition. Moreover, particles from 5% CFA mixture had the highest surface area.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity of Serratia marcescens strains as biological control agent: Implications for sustainable pest management.","authors":"Muhammad Rehan Akhtar, Muhammad Younas, Xiaofeng Xia","doi":"10.1111/1744-7917.13489","DOIUrl":"https://doi.org/10.1111/1744-7917.13489","url":null,"abstract":"<p><p>The escalating demand for sustainable and eco-friendly pest management strategies has raised interest in harnessing the pathogenic potential of microorganisms. Serratia marcescens, a Gram-negative bacterium, has emerged as a potential biological control agent for sustainable pest management. This review critically examines the history, biology, identification, and pathogenicity of S. marcescens strain with their potential application in pest management. The diverse mechanisms employed by the strain to exert control over pests, including the production of metabolites and the induction of systemic resistance in plants, are examined. The review also summarizes the ecological significance and global distribution of S. marcescens associated with the use of S. marcescens in biological control strategies. Furthermore, the usage efficacy of S. marcescens over other conventional chemicals is discussed. A comprehensive understanding of the pathogenic potential of S. marcescens strains as biological control agents is crucial for developing effective and sustainable pest management strategies. This review consolidates current research advances on S. marcescens, and provides insights into the prospects and challenges of using S. marcescens for integrated pest management.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-11903a modulates CLIPB9-mediated pathogen defense and longevity in Aedes aegypti.","authors":"Yan-Hui Liu, Ya-Nan Yin, Ling-Ling Yu, Meng-He Chang, Qian Han","doi":"10.1111/1744-7917.13512","DOIUrl":"https://doi.org/10.1111/1744-7917.13512","url":null,"abstract":"<p><p>Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases (CLIPs). In this study, it was confirmed that microRNA-11903a (miR-11903a) targets Aedes-CLIPB9 (AeCLIPB9) by bioinformatics prediction and dual-luciferase reporter assays. Following intrathoracic injection of miR-11903a agomir and antagomir, Real-time quantitative polymerase chain reaction confirmed that AeCLIPB9 is negatively regulated by miR-11903a. Spatiotemporal expression analysis revealed that miR-11903a is most abundant in 4th instar larvae, followed by pupae and adults, and highly expressed in the wings, head, and midgut of female adults. Following pathogen infection, AeCLIPB9 and miR-11903a exhibited opposite expression trends, indicating their potential roles in mosquito innate immunity. To further investigate the relationship between AeCLIPB9 and miR-11903a, double-strand CLIPB9 was synthesized and RNA interference was performed. Seven-d survival assays revealed that both AeCLIPB9 and miR-11903a were crucial immune factors in fighting pathogens. Finally, longevity assays demonstrated that miR-11903a influenced mosquito lifespan.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}