Insect Science最新文献_第2页

Ecological and social factors lead to variation in parental care between sexes in a burying beetle. 生态和社会因素导致了埋甲两性亲代抚育的差异。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70094

Donghui Ma, Long Ma, Jan Komdeur

{"title":"Ecological and social factors lead to variation in parental care between sexes in a burying beetle.","authors":"Donghui Ma, Long Ma, Jan Komdeur","doi":"10.1111/1744-7917.70094","DOIUrl":"https://doi.org/10.1111/1744-7917.70094","url":null,"abstract":"Sexual conflict over parental care represents a divergence in the evolutionary interests between males and females, consequently leading to distinct sex roles in parental care and reproductive strategies. However, whether and how various ecological and social environments influence such sex differences remains largely unclear. In this study, using the burying beetle Nicrophorus vespilloides, which breed on carcasses and exhibit biparental care pattern, we investigated the impacts of resource availability, intraspecific and interspecific competition on individual parental care and reproduction in males and females. We manipulated the resource availability for each breeding pair by providing either a large or a small mouse carcass. Furthermore, we constructed the presence of intraspecific and interspecific competition for pairs breeding on large carcasses, by introducing an additional, small pair of beetles and blowfly maggots Calliphora, respectively. We found that, compared with males and females breeding on large carcasses, males did not change their care, whereas females increased their care when breeding on small carcasses. In the presence of another pair of beetles, both males and females of the focal pair increased their care, whereas in the presence of blowfly maggots, they decreased their care. Our results showed that males and females adjust their parental care based on resource availability and competition pressure, with sex-specific differences driven by ecological and social factors. Our study sheds light on the importance of sex-specific parental care in response to various external drivers, which contributes to a better understanding of the evolution of sexual conflict over parental care.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The predatory behavior of ants: an impressive panoply of morphological adaptations. 蚂蚁的掠食行为：令人印象深刻的形态适应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70088

Alain Dejean, Xim Cerdá, Jérôme Orivel, Frédéric Azémar, Alexander L Wild, Bruno Corbara, Axel Touchard

{"title":"The predatory behavior of ants: an impressive panoply of morphological adaptations.","authors":"Alain Dejean, Xim Cerdá, Jérôme Orivel, Frédéric Azémar, Alexander L Wild, Bruno Corbara, Axel Touchard","doi":"10.1111/1744-7917.70088","DOIUrl":"https://doi.org/10.1111/1744-7917.70088","url":null,"abstract":"Ants are typically omnivorous insects with ovoid heads equipped with short mandibles, but there is great diversity in both the adaptations of their morphological and behavioral traits, as well as their dietary habits. Here, we review the variety of form and function in ant predation. Predation and scavenging were likely the plesiomorphic modes, with Cretaceous ants evolving mandibles well-suited to prey capture. In contemporary ground-dwelling species, mandible morphology can vary with adaptations to capturing particular prey as they may possess trap-jaw, snapping, or falciform mandibles characterized by their high closure speed. To capture termites or other ants, specialized ants may eliminate guards to prey on workers and brood or use allomones that cause the workers of raided nests to flee, providing access to the brood. Among arboreal ants, many species rely on vision to detect flying insects that land on their host trees. In tropical rainforests, territorially dominant arboreal ants (TDAAs) often hunt in groups, spreadeagling their prey, while venom use is noted in only a few species. Some obligate plant-ant species have developed ambush strategies for prey capture, building traps or hiding in shelters. Consequently, ants, due to their large number, their species diversity, and their capacity to live from the ground to tree crowns, regulate all kinds of arthropods through their predation. Ground-dwelling ants impact arthropods in the leaf-litter or provide biotic protection on small plants, but rarely on trees, while the TDAAs that occupy tree crowns protect their host trees from defoliating insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of transformer, transformer-2, and doublesex genes in the carambola fruit fly, Bactrocera carambolae, revealing potential for genetic pest management. 杨桃小实蝇（Bactrocera carambolae） transformer、transformer-2和双性基因的分子特征，揭示遗传害虫管理的潜力。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-24 DOI: 10.1111/1744-7917.70089

Kamoltip Laohakieat, Thanalai Poonsiri, Nidchaya Aketarawong

{"title":"Molecular characterization of transformer, transformer-2, and doublesex genes in the carambola fruit fly, Bactrocera carambolae, revealing potential for genetic pest management.","authors":"Kamoltip Laohakieat, Thanalai Poonsiri, Nidchaya Aketarawong","doi":"10.1111/1744-7917.70089","DOIUrl":"https://doi.org/10.1111/1744-7917.70089","url":null,"abstract":"Globalization and climate change may be driving the spread of the quarantine fruit fly, Bactrocera carambolae, highlighting the need for ecofriendly control methods like the Sterile Insect Technique (SIT), which relies on releasing sterile males to reduce wild populations. However, developing effective strains through genetic manipulation of sex-determination genes remains challenging due to limited knowledge of this pathway in B. carambolae. In this study, the structures of three key orthologous genes-transformer (tra), transformer-2 (tra-2), and doublesex (dsx)-were characterized and compared with those of other Bactrocera species. These genes were found to share conserved structures, following a bottom-up pattern from Bcardsx to Bcartra-2 and Bcartra, with intron retention observed in Bcartra as a structural variation. Developmental expression analysis revealed that the splicing patterns of Bcartra first appeared at 6 hours post oviposition (hpo), with sex-specific patterns established by 10 hpo. The expression profiles in B. carambolae were similar to those in the closely related species B. dorsalis, although gene expression occurred later in B. carambolae. RNAi knockdown of Bcartra resulted in nearly all phenotypic males, supporting the role of BcarTRA in controlling the sex-specific splicing of Bcartra itself and the downstream genes doublesex and fruitless. Additionally, in silico predictions of protein interactions (TRAF, TRA-2, and RBP1) and putative cis-regulatory elements on Bcartra pre-mRNA suggested specific binding events occur at these conserved sites. These findings contribute to the development of potential genetic tools for pest control and provide insights into the evolutionary relationships among these orthologous genes.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and phenotypic variation in wood tiger moths from the Caucasus: insights into male warning color variation. 高加索地区木虎蛾的遗传和表型变异：对雄性警告色变异的见解。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-19 DOI: 10.1111/1744-7917.70101

Juan A Galarza, Ossi Nokelainen, Melanie N Brien, Cristina Ottocento, Bibiana Rojas, Janne K Valkonen, Tamar Chunashvili, Tõnis Tasane, Chris D Jiggins, Johanna Mappes

{"title":"Genetic and phenotypic variation in wood tiger moths from the Caucasus: insights into male warning color variation.","authors":"Juan A Galarza, Ossi Nokelainen, Melanie N Brien, Cristina Ottocento, Bibiana Rojas, Janne K Valkonen, Tamar Chunashvili, Tõnis Tasane, Chris D Jiggins, Johanna Mappes","doi":"10.1111/1744-7917.70101","DOIUrl":"10.1111/1744-7917.70101","url":null,"abstract":"Coloration plays a pivotal role in shaping how species adapt to their environment, influencing their interactions with predators, prey, and potential mates. The aposematic wood tiger moth (Arctia plantaginis) is sexually dimorphic. Males are polymorphic in their hindwing coloration across the Holarctic distribution range, while females exhibit continuous variation in their coloration. In the Caucasus region, a striking exception can be found, where both sexes exhibit a continuous orange-red hindwing coloration. Yet, it remains uncertain whether significant color variations exist within the spectrum of male orange-red coloration and whether these differences can be associated with genetic structure or other phenotypic traits such as size. Using population genetics and image analyses methods, we find that males from the Lesser Caucasus have predominantly large red wings and constitute mostly a single genetic population. Males from the Greater Caucasus, in contrast, appear genetically isolated and are relatively small with orange hindwings. We discuss these findings in the context of both contemporary and historical environmental factors that may have influenced male color variation in the region.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction of Buchnera with rifampicin impairs the density-dependent induction of winged morph in pea aphid. 用利福平减少布氏线虫对豌豆蚜虫翅形诱导的密度依赖性影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-17 DOI: 10.1111/1744-7917.70098

Erliang Yuan, Bingru Du, Yucheng Sun

{"title":"Reduction of Buchnera with rifampicin impairs the density-dependent induction of winged morph in pea aphid.","authors":"Erliang Yuan, Bingru Du, Yucheng Sun","doi":"10.1111/1744-7917.70098","DOIUrl":"https://doi.org/10.1111/1744-7917.70098","url":null,"abstract":"Wing dimorphism is a critical trait that helps insects better adapt to environments. High population density and poor nutrition are known to induce winged morph in aphids. Buchnera aphidicola, an obligate endosymbiont of aphids, supplies the host with essential amino acids and B vitamins, while the mechanistic basis of Buchnera's role in density-dependent production of winged aphids is largely unclear. Reduction of Buchnera from maternal aphids or the 1st instar nymphs with antibiotic rifampicin reduced the proportion of winged aphids. In contrast, for the 2nd instar nymphs, reduction of Buchnera failed to decline the proportion of winged aphids, suggesting the 1st instar is a critical period for wing plasticity. Genes associated with amino acid transport and insulin signaling were identified by RNA sequencing as differentially expressed genes between rifampicin and dimethyl sulfoxide-treated 1st instar nymphs. Proton-assisted amino acid transporter 1 (PAT1), was down-regulated by rifampicin. Knockdown of PAT1 in 1st instar nymphs reduced the proportion of winged aphids. In addition, reduction of Buchnera with rifampicin up-regulated several marker genes of the insulin signaling pathway, and reduced the phosphorylation level of forkhead transcription factor subgroup O (FOXO), a determinant of wing morph downstream of insulin signaling. Application of FOXO phosphorylation inhibitor LY2780301 decreased the proportion of winged aphids, while the agonist SC79 increased the proportion of winged aphids. These results revealed that reduction of Buchnera sharply declined the proportion of winged aphids, indicating that the vertical transmission of Buchnera could bring on a maternal signal of crowding perception, which was required for the production of winged offspring.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the key regulatory network and genes highly associated with the synthesis of cantharidin in Epicauta chinensis by transcriptome and metabolome analyses. 利用转录组学和代谢组学分析鉴定中华壁蚌中斑蝥素合成的关键调控网络和高度相关基因。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-17 DOI: 10.1111/1744-7917.70102

Xing Tian, Shirong Li, Dong Cheng, Di Tong, Ting Zhou, Lei Yi, Yalin Zhang, Shumin Lü

{"title":"Identification of the key regulatory network and genes highly associated with the synthesis of cantharidin in Epicauta chinensis by transcriptome and metabolome analyses.","authors":"Xing Tian, Shirong Li, Dong Cheng, Di Tong, Ting Zhou, Lei Yi, Yalin Zhang, Shumin Lü","doi":"10.1111/1744-7917.70102","DOIUrl":"https://doi.org/10.1111/1744-7917.70102","url":null,"abstract":"Cantharidin, as a defensive toxin of blister beetles, has attracted widespread attention in the agricultural and medical fields. However, knowledge about the cantharidin biosynthetic pathway remains limited to date. In this study, we performed transcriptome and metabolome analyses of fat bodies in Epicauta chinensis to reveal the key genes and metabolites related to cantharidin biosynthesis. The identified differentially expressed genes and differentially accumulated metabolites were primarily enriched in biosynthesis of secondary metabolites and metabolic pathways. Through weighted gene co-expression network analysis, 327 genes consistent with the variation of cantharidin content were identified, and cytochrome P450s (CYPs) and phytanoyl-CoA dioxygenase (Phyh) were novel candidate genes due to their high correlation with hub genes involved in cantharidin biosynthesis. Metabolome profiling identified the key cantharidin precursor isopentenyl diphosphate (IPP). The results of integrated analysis revealed that 49 and 11 genes interact with IPP and cantharidin, respectively. Correlation analysis showed that EcCYP6BK33, EcCYP4TT1, and EcPhyh were positively and significantly correlated with cantharidin. Furthermore, knockdown of EcCYP4TT1 and EcPhyh led to significant reductions of cantharidin synthesis; however, RNA interference of EcCYP6BK33 had no significant influence on cantharidin synthesis. These results indicate the importance of fat body in cantharidin biosynthesis. Together, our results also reveal a series of candidate genes for cantharidin biosynthesis, and generated transcriptome and metabolome data as well as a gene regulatory network, which are valuable resources for future characterization of cantharidin biosynthesis in blister beetles.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal effects of chlorantraniliprole on sex pheromone communication behavior of male Athetis lepigone by upregulating odorant-binding protein 20. 氯虫腈通过上调气味结合蛋白20对雄性雪蚤性信息素交流行为的亚致死效应

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-17 DOI: 10.1111/1744-7917.70093

Jian-Qiao Li, Xiao-Min Wu, Yu-Meng Zhu, Lu Xu, Wen-Wen Zhu, Xin-Yao Sun, Jin-Bu Li, Raul Narciso C Guedes, Xiu-Yun Zhu, Youssef Dewer, Ya-Nan Zhang

{"title":"Sublethal effects of chlorantraniliprole on sex pheromone communication behavior of male Athetis lepigone by upregulating odorant-binding protein 20.","authors":"Jian-Qiao Li, Xiao-Min Wu, Yu-Meng Zhu, Lu Xu, Wen-Wen Zhu, Xin-Yao Sun, Jin-Bu Li, Raul Narciso C Guedes, Xiu-Yun Zhu, Youssef Dewer, Ya-Nan Zhang","doi":"10.1111/1744-7917.70093","DOIUrl":"https://doi.org/10.1111/1744-7917.70093","url":null,"abstract":"Sex pheromone communication is critical for moth reproduction, relying on olfactory genes such as odorant-binding proteins (OBPs) expressed in the antennae. This study investigates how chlorantraniliprole stress impacts the sex pheromone communication behaviors of male Athetis lepigone. We found that chlorantraniliprole inhibited calling and mating behaviors. To uncover the underlying mechanism, we identified AlepOBP20, a gene significantly upregulated under insecticide stress, and characterized its binding affinities with sex pheromones and chlorantraniliprole using a series of in vitro methods. Functional analyses through RNA interference, electrophysiology, and behavioral assays confirmed the pivotal role of AlepOBP20 in male moths responding to sublethal chlorantraniliprole stress. These findings suggest AlepOBP20 may help male moths adapt to chlorantraniliprole stress, offering a scientific basis for developing innovative pest control strategies targeting OBPs.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of TYLCV infection on host selection and feeding behavior of MED Bemisia tabaci on pepper plants. TYLCV侵染对辣椒烟粉虱寄主选择及取食行为的影响

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-17 DOI: 10.1111/1744-7917.70090

Yang Zhong, Jingyu Guo, Yan Li, Yaqi Wen, Jie Hu, Baiming Liu, Qingjun Wu, Xiaoguo Jiao

{"title":"Impact of TYLCV infection on host selection and feeding behavior of MED Bemisia tabaci on pepper plants.","authors":"Yang Zhong, Jingyu Guo, Yan Li, Yaqi Wen, Jie Hu, Baiming Liu, Qingjun Wu, Xiaoguo Jiao","doi":"10.1111/1744-7917.70090","DOIUrl":"https://doi.org/10.1111/1744-7917.70090","url":null,"abstract":"The tomato yellow leaf curl virus (TYLCV), spread by the vector insect Bemisia tabaci, dramatically reduces worldwide tomato production and quality. Plant-mediated interactions between TYLCV and vector whiteflies commonly occur when previous infestations induce both volatile and nonvolatile changes in the shared host. These induced changes of the shared host may differentially affect subsequent interacting species within the plant-insect-virus tripartite system. These altered volatile profiles can be perceived as airborne cues that enhance or suppress defense responses in neighboring conspecifics, a phenomenon known as \"plant-plant communication.\" Here, we present the first evidence that TYLCV-infected Mediterranean (MED) B. tabaci (hereafter referred to as viruliferous MED) preferentially settles and oviposits on previously infested peppers, an alternative host for TYLCV, over those merely exposed and intact ones. Furthermore, differences in feeding behavior of viruliferous MED, assessed using an electrical penetration graph, clearly indicate that these MED whiteflies feed more effectively on previously infested and exposed peppers than on healthy ones. Our results suggest that the suppression of pepper defenses-both nonvolatile and volatile-by previous infestation and exposure to viruliferous MED promotes alternative host-mediated mutualism between MED and TYLCV. These findings provide insight into peppers, functioning as alternative hosts, mediating the interactions between TYLCV and its vector MED whiteflies. Moreover, our results may enhance the understanding of TYLCV epidemics, particularly in the context of the rapid invasion of MED B. tabaci, thereby facilitating the development of novel management strategies for controlling this viral pathogen and its devastating whiteflies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

20-hydroxyecdysone-mediated regulation of the ribosomal protein Cs40SRPS4 via CsE74A is essential for vitellogenesis in Chilo suppressalis. 20-羟基蜕皮激素通过CsE74A介导的核糖体蛋白Cs40SRPS4的调节对Chilo抑制症的卵黄形成至关重要。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-12 DOI: 10.1111/1744-7917.70092

Jingxuan Hu, Qingzhen He, Yingjuan Sun, Wenbing Ding, Hualiang He, Hongshuai Gao, Qiao Gao, Lin Qiu, Youzhi Li

{"title":"20-hydroxyecdysone-mediated regulation of the ribosomal protein Cs40SRPS4 via CsE74A is essential for vitellogenesis in Chilo suppressalis.","authors":"Jingxuan Hu, Qingzhen He, Yingjuan Sun, Wenbing Ding, Hualiang He, Hongshuai Gao, Qiao Gao, Lin Qiu, Youzhi Li","doi":"10.1111/1744-7917.70092","DOIUrl":"https://doi.org/10.1111/1744-7917.70092","url":null,"abstract":"Development and reproduction are key processes in the insect life cycle, and vitellogenesis is a critical event in insect reproduction. Vitellogenin (Vg) is generally recognized as a female-specific, reproductive lipoprotein unique to oviparous taxa that plays a crucial role in vitellogenesis. However, its specific functions, and the mechanisms underlying its regulation, remain poorly understood. We here demonstrate how 20-hydroxyecdysone (20E) regulates Vg transcription via the transcription factor CsE74A and the ribosomal protein Cs40SRPS4 in Chilo suppressalis. Disruption of CsVg in G0 generation C. suppressalis via multiplex CRISPR/Cas9 resulted in decreased yolk deposition, ovarian dysplasia, and delayed oocyte maturation. Dual luciferase and DNA pull-down assays identified the CsVg core promoter (-915 to -811 bp) and its binding ribosomal protein Cs40SRPS4. Functional analysis via RNA interference revealed that Cs40SRPS4 is a ribosomal protein capable of regulating vitellogenesis in C. suppressalis. Furthermore, we correctly predicted that the binding site for CsE74A would be on the CsVg core promoter and confirmed its binding capability. Subsequent experiments further confirmed the interaction between CsE74A and Cs40SRPS4. Notably, the expression of CsE74A, Cs40S4, and CsVg significantly increased after 20E treatment. The results revealed that 20E induced the early response factor CsE74A to bind to the CsVg core promoter, thereby regulating Vg transcription and vitellogenesis in conjunction with Cs40SRPS4. These findings not only provide a foundation for gaining deeper understanding of insect reproductive processes, but could also lead to the development of safer, more economical and sustainable, pest control methods.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue on improvement of colony management in insect mass-rearing for sterile insect technique applications-foreword. 面向昆虫不育技术应用的昆虫群体管理改进特刊前言。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-12 DOI: 10.1111/1744-7917.70086

引用次数: 0