Insect Science最新文献_第2页

Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes. 不可胜选扶桑的遗传结构、扩散途径和向北扩张预测：中国不同景观的20年之旅。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-21 DOI: 10.1111/1744-7917.70133

Jun Huang, Chen-Di Yu, Shi-Zhen Wang, Jian-Dong Bao, Zhi-Tao Jiang, Wan-Ying Dong, Hong-Li Su, Li-Ming Chen, Farman Ullah, Shu-Xing Zhou, Xiao-Wei Li, Jin-Ming Zhang, Zhi-Jun Zhang, Yao-Bin Lu, Juan Zhang

{"title":"Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes.","authors":"Jun Huang, Chen-Di Yu, Shi-Zhen Wang, Jian-Dong Bao, Zhi-Tao Jiang, Wan-Ying Dong, Hong-Li Su, Li-Ming Chen, Farman Ullah, Shu-Xing Zhou, Xiao-Wei Li, Jin-Ming Zhang, Zhi-Jun Zhang, Yao-Bin Lu, Juan Zhang","doi":"10.1111/1744-7917.70133","DOIUrl":"https://doi.org/10.1111/1744-7917.70133","url":null,"abstract":"The red imported fire ant, Solenopsis invicta, has invaded China for over two decades, advancing northwest from Guangdong to the Yangtze River coast at 30.8° N latitude. A decade since the last genetic analysis, this study revisits the species' genetic structure and invasion trajectory amidst intensifying selective pressures. Through mitochondrial DNA resequencing of 96 samples across 6 Chinese provinces, we identified 2 haplotypes (H5 and H22), with H22 predominating in Zhejiang, the northern limit of our study. Phylogenetic analysis suggests a likely invasion source from Guangdong and Fujian, with long-distance dispersal facilitated by the horticultural trade, particularly Cycas revoluta. Local dispersal rates varied significantly, with 40 m/year in northern Zhejiang and 118-186 m/year in Guangdong. A regression model (y = -303.2523 + 10 855.1813/x) forecasts a northern expansion threshold near southern Shandong. However, considering the bridgehead effect, repeated invasions, and climate change, the species' invasive range may extend further north.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transovarial transmission of Wolbachia bacteria via P44/Msp2-IMP2 mediated endocytosis. 通过P44/Msp2-IMP2介导的胞吞作用传播沃尔巴克氏菌。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-18 DOI: 10.1111/1744-7917.70129

Lu Ren, Yan-Jun Men, Xiao-Li Bing, Ary Hoffmann, Xiao-Yue Hong

{"title":"Transovarial transmission of Wolbachia bacteria via P44/Msp2-IMP2 mediated endocytosis.","authors":"Lu Ren, Yan-Jun Men, Xiao-Li Bing, Ary Hoffmann, Xiao-Yue Hong","doi":"10.1111/1744-7917.70129","DOIUrl":"https://doi.org/10.1111/1744-7917.70129","url":null,"abstract":"Wolbachia is a maternally inherited endosymbiont that stably localizes in oocytes of arthropods to ensure successful transovarial transmission. However, the source of Wolbachia in oocytes is unclear. Here, we obtained a Wolbachia strain that is transovarially transmitted with complete fidelity in the agricultural pest spider mite Tetranychus truncatus. Using fluorescence in situ hybridization, we showed that Wolbachia migrate from the digestive cells to the reproductive system as a female develops. When Wolbachia from T. truncatus was transferred to Aedes albopictus cells, we demonstrated that its cell-to-cell spread was regulated by the host's endocytosis system. By assaying the proteome, we identified a bacterial surface protein P44/Msp2 from Wolbachia that interacted with the host's integral membrane protein 2 (IMP2). RNA interference of IMP2 indicated that it facilitated entry of Wolbachia. Our results show that Wolbachia from somatic cells contribute to its transovarial transmission, and that Wolbachia also utilize the surface protein to invade new host cells. This extends our understanding of how Wolbachia is transmitted between cells and facilitates artificial transfection work.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual mechanisms of olfactory plasticity: Transient modulation by phosphatidylcholine and D-chiro-inositol and sustained regulation via the sex peptide-juvenile hormone system. 嗅觉可塑性的双重机制：磷脂酰胆碱和d -肌醇的瞬时调节和性肽-幼年激素系统的持续调节。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70114

Xiaoqing Yao, Chang Luo, Shupei Ai, Fei Cheng, Shuyuan Xing, Guohua Zhong, Xin Yi

{"title":"Dual mechanisms of olfactory plasticity: Transient modulation by phosphatidylcholine and D-chiro-inositol and sustained regulation via the sex peptide-juvenile hormone system.","authors":"Xiaoqing Yao, Chang Luo, Shupei Ai, Fei Cheng, Shuyuan Xing, Guohua Zhong, Xin Yi","doi":"10.1111/1744-7917.70114","DOIUrl":"https://doi.org/10.1111/1744-7917.70114","url":null,"abstract":"Insects exhibit olfactory plasticity to optimize their survival and reproduction, which ensures that their sensory preferences flexibly meets their needs. However, a comprehensive understanding of the sequential regulatory mechanisms driving these sensory adjustments is lacking. Addressing this knowledge gap is crucial for clarifying how the olfactory system of insect flexibly meets their reproductive needs. Using electroantennogram (EAG) and single-sensillum recordings, we found that female Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) displayed distinct olfactory preferences for oviposition cues, host volatiles, and sex pheromones before and after mating. The injection of synthesized sex peptide (SP) or juvenile hormone (JH) into virgin females, as well as the knockdown of SP- or JH-receptors in mated females, reversed the olfactory preferences in both groups of flies. The onset of the effect of JH was relatively gradual, as evidenced by the lack of changes observed during the first 6 h after mating. The rapid changes in antennal sensitivities may involve specific neuromolecules, such as phosphatidylcholine (PC) and D-chiro-inositol (DCI), which were identified through metabolomics screening, and their functions were further validated by microinjection. Our findings reveal a post-mating regulatory mechanism where immediate olfactory adjustments are facilitated by rapid signaling molecules, while long-term sensitivity modifications rely on the gradual action of JH.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing of juvenile hormone-related genes through RNA interference leads to molt failure and high mortality in the spongy moth. 通过RNA干扰使幼体激素相关基因沉默导致海绵蛾蜕皮失败和高死亡率。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70117

Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi

{"title":"Silencing of juvenile hormone-related genes through RNA interference leads to molt failure and high mortality in the spongy moth.","authors":"Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi","doi":"10.1111/1744-7917.70117","DOIUrl":"https://doi.org/10.1111/1744-7917.70117","url":null,"abstract":"Lymantria dispar (spongy moth) is a globally polyphagous and destructive pest, with the larval stage being the main destructive stage. The larvae feed on more than 600 species of plants ranging from oaks to conifers, and they are capable of completely defoliating entire trees, leading to significant ecological and economic losses. Chemical insecticides are commonly used to control them, but these methods are expensive and nonspecific. Therefore, RNA interference (RNAi) is being explored as an alternative strategy to control spongy moth. Juvenile hormones (JHs) play a crucial role in regulating insect growth, diapause, and reproduction, making them potential targets for pest control. This study investigated whether 2 enzymes involved in the JH pathways-JH acid methyltransferase (JHAMT) from the biosynthetic pathway and JH epoxide hydrolase (JHEH) from the metabolic pathway-could be used for RNAi-based control. Ldjhamt showed predominant expression during the larval stages and Ldjheh exhibited high expression throughout development. Both genes had the highest expression in the fat body. RNAi silencing using specific double-stranded RNAs resulted in a significant decrease in the expression level of target genes, as well as a significant reduction in larval survival rate, pupation rate and eclosion rate. Additionally, many larvae and pupae died due to developmental abnormalities, and the adult size of the treated group was smaller than the control group. These findings demonstrate that jhamt and jheh are vital for the growth and development of spongy moths and suggest they could be effective targets for RNAi-based pest control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a dual-loop double-stranded RNA to control small brown planthopper. 双环双链RNA在小褐飞虱控制中的应用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70128

Panpan Ge, Xiangyang Lu, Wan Zhao, Shaohui Wu, Neil Smith, Ling-Ling Gao, Hui-Shan Guo, Ming-Bo Wang, Feng Cui

{"title":"Application of a dual-loop double-stranded RNA to control small brown planthopper.","authors":"Panpan Ge, Xiangyang Lu, Wan Zhao, Shaohui Wu, Neil Smith, Ling-Ling Gao, Hui-Shan Guo, Ming-Bo Wang, Feng Cui","doi":"10.1111/1744-7917.70128","DOIUrl":"https://doi.org/10.1111/1744-7917.70128","url":null,"abstract":"RNA interference, induced by double-stranded RNA (dsRNA), has emerged as a biopesticide for pest control. However, the stability of dsRNA remains a challenge in topical delivery, preventing widespread agricultural application. Here, we tested a dsRNA variant, called loop-ended dsRNA or LedRNA, for controlling a notorious vector insect, the small brown planthopper (SBPH). LedRNA is characterized by having a loop at both ends of the dsRNA stem, resembling a close-circled RNA. Three SBPH target genes, including Flotillin2, which is vital for viral transmission, and trehalose-6-phosphate synthase (TPS) and malate dehydrogenases (MDH), which are essential for insect survival, were tested for gene silencing efficiencies through microinjection, soaking, or oral delivery of the conventional dsRNA, hairpin dsRNA, and LedRNA. All 3 molecules induced effective gene silencing using microinjection, but did not suppress target gene expression through soaking. Notably, LedRNA demonstrated superior efficacy in inducing gene silencing in SBPH through oral feeding compared to the other 2 molecules. Silencing efficiencies ranged from 26% to 39% for Flotillin2, 39% to 51% for TPS, and 45% to 64% for MDH. When SBPH were fed Escherichia coli-synthesized LedRNA targeting these 3 genes, silencing efficiencies ultimately reached 28% to 74%, leading to reduced insect survival and decreased viral transmission in host plant rice. This study highlights the potential of LedRNA as a biopesticide for insect pest control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two transcription factor cascades regulate odorant-binding protein NlOBP8 affecting the recognition of linalool in the brown planthopper Nilaparvata lugens. 两个转录因子级联调节气味结合蛋白NlOBP8，影响褐飞虱对芳樟醇的识别。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70118

Ruilin Zhang, Shuanggang Duan, Sara Taha Abdelkhalek, Ao Liu, Chao Wang, Yi Wei, Ruinan Yang, Manqun Wang

{"title":"Two transcription factor cascades regulate odorant-binding protein NlOBP8 affecting the recognition of linalool in the brown planthopper Nilaparvata lugens.","authors":"Ruilin Zhang, Shuanggang Duan, Sara Taha Abdelkhalek, Ao Liu, Chao Wang, Yi Wei, Ruinan Yang, Manqun Wang","doi":"10.1111/1744-7917.70118","DOIUrl":"https://doi.org/10.1111/1744-7917.70118","url":null,"abstract":"The insect olfactory system is pivotal in mediating chemical-driven behaviors, including host plant selection, mate identification, oviposition site localization, and ecological niche recognition. Although odorant-binding proteins (OBPs) are known to facilitate olfactory signal transduction in insects, the transcriptional regulatory mechanisms governing their expression remain elusive. Here, we identified 2 transcriptional cascades involving the transcription factors (TFs) distal-less (Dll) and aristaless (al) that converge to regulate NlObp8 expression in the brown planthopper (Nilaparvata lugens, BPH) through bioinformatics and transcriptomic analysis. The functional impact of the 2 TFs was validated using the dual-luciferase reporter assays and yeast 1-hybrid assays. RNA interference-mediated knockdown of Nlal significantly downregulated NlObp8 expression and abolished the repellent behavioral response of BPHs to linalool. Furthermore, silencing of NlDll resulted in significant downregulation of Nlal expression. These results revealed that the cascade regulatory mechanism, involving direct al binding and indirect Dll-mediated transcriptional activation, provided molecular targets for disrupting olfactory-driven behaviors in agricultural pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond strain maintenance: Pre-Filter, a viable practice to incorporate desirable traits to genetic sexing strains (GSS) of Ceratitis capitata used in sterile insect technique (SIT) control programs. 在菌种维持之外：预过滤是一种可行的方法，可以将理想的性状纳入到不育昆虫技术（SIT）控制程序中使用的头角杆菌遗传性别菌株（GSS）中。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-14 DOI: 10.1111/1744-7917.70091

Edwin Mauricio Ramírez-Santos, Pedro Alfonso Rendón Arana, Lested Darío Rivas-González, Raúl Menéndez Cahueque

{"title":"Beyond strain maintenance: Pre-Filter, a viable practice to incorporate desirable traits to genetic sexing strains (GSS) of Ceratitis capitata used in sterile insect technique (SIT) control programs.","authors":"Edwin Mauricio Ramírez-Santos, Pedro Alfonso Rendón Arana, Lested Darío Rivas-González, Raúl Menéndez Cahueque","doi":"10.1111/1744-7917.70091","DOIUrl":"https://doi.org/10.1111/1744-7917.70091","url":null,"abstract":"The sterile insect technique (SIT) for controlling the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), requires the mass-rearing of males in bio-facilities. This study summarizes the implementation of a Pre-Filter system in the mass-rearing process of C. capitata at the Moscamed program's El Pino facility in Guatemala. The Pre-Filter selection process as presented here was aimed to increase the longevity of the VIENNA 8 D53- strain males released for pest control. Over a 9-year period (2015-2024), the longevity of these males was systemically monitored, and the impact of the Pre-Filter system on mortality, half-life, and survival rate were analyzed. Results indicated a significant reduction in mortality at 48 h, declining from 16.47% in 2017 to 2.56% in 2024. Concurrently, the half-life of males increased from 79.55 h in 2018 to 85.97 h in 2024. The Pre-Filter system shifted the frequency distribution of half-lives toward longer durations, with a peak observed between 76.14 and 86.14 h, whereas populations without the Pre-Filter exhibited shorter half-lives, peaking between 56.14 and 66.14 h. Comparative analyses with historical data (2005-2024) demonstrated that the Pre-Filter system significantly improved the male longevity, compared to pre-implementation periods during which mortality rates were higher and more variable. Additionally, the results revealed a gradual reduction in longevity variability within the population, indicating a more homogeneous longevity among the mass-reared males.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term rearing experiences influence parasitism ability of an ectoparasitoid wasp. 长期饲养对寄生蜂的寄生能力有影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-08 DOI: 10.1111/1744-7917.70115

Shumei Pan, Chunxia Wang, Jing Su, Xiangqian Huang, Shiyao Liu, Haodong Hao, Xiuju Yu, Hongxia Li, Jianghua Sun, Li Li, Zhudong Liu

{"title":"Long-term rearing experiences influence parasitism ability of an ectoparasitoid wasp.","authors":"Shumei Pan, Chunxia Wang, Jing Su, Xiangqian Huang, Shiyao Liu, Haodong Hao, Xiuju Yu, Hongxia Li, Jianghua Sun, Li Li, Zhudong Liu","doi":"10.1111/1744-7917.70115","DOIUrl":"https://doi.org/10.1111/1744-7917.70115","url":null,"abstract":"Parasitoid wasps face increased mortality when paralyzing resistant hosts, involving host adaptation and biocontrol. This study investigates the plasticity of host adaptation in the ectoparasitoid Sclerodermus guani by examining how long-term rearing on hosts of differing resistance influences parasitism performance. Two distinct wasp lineages were established: the stressful Ma lineage, continuously reared on the highly resistant Monochamus alternatus (Ma) larvae, and the susceptible Sp lineage, continuously reared on the non-resistant Saperda populnea (Sp) larvae. Host preference assays revealed that Ma wasps exhibited a significant preference for Ma larvae, while Sp wasps favored Sp larvae. Although Ma wasps experienced higher mortality and a lower population increase index when reared on Ma larvae compared to the performance of Sp wasps on Sp larvae, they demonstrated superior parasitism efficiency when tested against resistant hosts. Specifically, Ma wasps had significantly lower mortality rates and higher population increase index than Sp wasps when both were tested on Ma larvae. In dual-parasitism trails, where 1 Ma wasp and 1 Sp wasp were introduced to the same Ma larvae, and in group trails involving 3 wasps from each lineage on the novel, high-resistant host Aromia bungii, the Ma wasps outperformed Sp wasps in paralysis success. Behavioral assays further indicated that Ma wasps invested more time in accessing host resistance but were quicker in locating hosts compared to Sp wasps. This research provides valuable insights for improving the design of mass rearing strategies to maximize the effectiveness of biological control agents in integrated pest management programs.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenerational effects of heavy metal contamination on two Trichogramma egg parasitoids and potential impacts on biological control. 重金属污染对两种赤眼蜂卵寄生蜂的跨代影响及其生物防治的潜在影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-07 DOI: 10.1111/1744-7917.70125

Jie Wang, Ning Di, Cheng-Xing Wang, Zheng-Yang Zhu, Sean M Prager, He-Xi Huang, John T Trumble, Nicolas Desneux, Yuan-Xi Li, Su Wang

{"title":"Transgenerational effects of heavy metal contamination on two Trichogramma egg parasitoids and potential impacts on biological control.","authors":"Jie Wang, Ning Di, Cheng-Xing Wang, Zheng-Yang Zhu, Sean M Prager, He-Xi Huang, John T Trumble, Nicolas Desneux, Yuan-Xi Li, Su Wang","doi":"10.1111/1744-7917.70125","DOIUrl":"https://doi.org/10.1111/1744-7917.70125","url":null,"abstract":"The increased basic knowledge of biological control agents' bio-ecology under environmental stress provides useful information for optimizing integrated pest management. With an increasing awareness of cadmium (Cd) pollution in agroecosystems, this study evaluated the transgenerational effects and host preference of two widely distributed and utilized egg parasitoids, Trichogramma dendrolimi and Trichogramma japonicum through bottom-up effects mediated by Cd. The host rice moth Corcyra cephalonica was reared on a diet with 20 mg/kg Cd exposure for 1 generation; eggs were collected, and then exposed to Trichogramma. The survival rate, longevity, daily number of parasitized eggs, and fecundity of second generation (F1) females, emergence rate, and female adult proportion of the third generation (F2) were tested. After emerging from Cd-exposed hosts, the survival rate and longevity of F1 T. dendrolimi female adults were significantly increased, but the daily number of eggs parasitized by, and fecundity of, F1 T. japonicum were significantly decreased. Cd exposure significantly resulted in a preference for hosts exposed to Cd in both F1 T. dendrolimi and T. japonicum, whereas no such host preference was observed when the first generation (F0) parasitoids were not exposed to Cd during their development. Our study shows the transgenerational effects and host preference on natural enemies under heavy metal exposure stress in a species-specific way. This highlights the potential synergetic effects of heavy metal contamination on biological control. Specifically, the release of T. dendrolimi, rather than T. japonicum, should be favored at Cd-contaminated sites, where T. dendrolimi is a more suitable biocontrol agent.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of CYP4Gs involved in hydrocarbon formation in the survival, reproduction, and pyrethroid resistance of Triatoma infestans, a major Chagas disease vector. CYP4Gs在恰加斯病主要媒介——斑鼻虫（Triatoma infestans）的生存、繁殖和拟除虫菊酯抗性中参与碳氢化合物形成的作用

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-07-07 DOI: 10.1111/1744-7917.70100

Nicolás Jesús Nazareth Ifrán, Sergio Javier Mijailovsky, Marianela Santana, Carolina Remón, Juan Roberto Girotti, Gustavo Mario Calderón-Fernández

{"title":"The role of CYP4Gs involved in hydrocarbon formation in the survival, reproduction, and pyrethroid resistance of Triatoma infestans, a major Chagas disease vector.","authors":"Nicolás Jesús Nazareth Ifrán, Sergio Javier Mijailovsky, Marianela Santana, Carolina Remón, Juan Roberto Girotti, Gustavo Mario Calderón-Fernández","doi":"10.1111/1744-7917.70100","DOIUrl":"https://doi.org/10.1111/1744-7917.70100","url":null,"abstract":"Very long-chain normal and branched hydrocarbons are essential for insect physiology and ecology. The final metabolic step of hydrocarbon formation involves an oxidative decarbonylation carried out by cytochrome P450 from family 4, subfamily G (CYP4G). Triatoma infestans Klug (Hemiptera: Reduviidae: Triatominae) has two CYP4G genes, named CYP4G106 and CYP4G107. In this study, we have silenced in T. infestans the expression of both genes by RNA interference in order to assess the relevance of hydrocarbons to survival, reproductive physiology, and insecticide susceptibility. Gene knockdown led to a significant decrease (≈ 79%) in total cuticle hydrocarbons, especially normal alkanes. A reduced number of CYP4G-silenced nymphs completed metamorphosis, and adult survival-particularly among females-was markedly diminished. Although mating behavior was not affected by silencing, the reproductive fitness was severely impaired as CYP4G-silenced females laid about 77% fewer eggs per female than control females. Egg hatching was almost completely abolished by CYP4G-silencing. Furthermore, CYP4G gene knockdown increased susceptibility to the insecticide deltamethrin, leading to a 40%-50% higher mortality compared to control insects. These findings highlight the essential role of CYP4G genes in cuticle function, survival, and reproductive fitness and suggest that disruption of hydrocarbon biosynthesis can enhance insecticide efficacy, making it a potential target for control of these insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0