Insect Science最新文献_第2页

Eocene and modern entomofauna differ-a Cretaceous-like larva in Rovno amber. 始新世与现代内动物的区别--罗夫诺琥珀中的白垩纪幼虫。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-15 DOI: 10.1111/1744-7917.13410

Joachim T Haug, Simon Linhart, Viktor Baranov, Carolin Haug

引用次数: 0

Involvement of bacteria in the development of fungal infections in the Colorado potato beetle. 细菌参与科罗拉多马铃薯甲虫真菌感染的发展。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-02 DOI: 10.1111/1744-7917.13414

Vadim Yu Kryukov, Elena Kosman, Irina Slepneva, Yana L Vorontsova, Olga Polenogova, Gleb Kazymov, Tatyana Alikina, Yuriy Akhanaev, Darya Sidorenko, Yury A Noskov, Anton Krivopalov, Marsel R Kabilov, Olga Yaroslavtseva

{"title":"Involvement of bacteria in the development of fungal infections in the Colorado potato beetle.","authors":"Vadim Yu Kryukov, Elena Kosman, Irina Slepneva, Yana L Vorontsova, Olga Polenogova, Gleb Kazymov, Tatyana Alikina, Yuriy Akhanaev, Darya Sidorenko, Yury A Noskov, Anton Krivopalov, Marsel R Kabilov, Olga Yaroslavtseva","doi":"10.1111/1744-7917.13414","DOIUrl":"10.1111/1744-7917.13414","url":null,"abstract":"Entomopathogenic fungi may interact with insects' symbiotic bacteria during infection. We hypothesized that topical infection with Beauveria bassiana may alter the microbiota of the Colorado potato beetle (CPB) and that these modifications may alter the course of mycoses. We used a model with two concentrations of conidia: (1) high concentration that causes rapid (acute) pathogenesis with fast mortality followed by bacterial decomposition of insects; (2) lower concentration that leads to prolonged pathogenesis ending in conidiation on cadavers. The fungal infections increased loads of enterobacteria and bacilli on the cuticle surface and in hemolymph and midgut, and the greatest increase was detected during the acute mycosis. By contrast, stronger activation of IMD and JAK-STAT signaling pathways in integuments and fat body was observed during the prolonged mycosis. Relatively stable (nonpathogenic) conditions remained in the midgut during both scenarios of mycosis with slight changes in bacterial communities, the absence of mesh and stat expression, a decrease in reactive oxygen species production, and slight induction of Toll and IMD pathways. Oral administration of antibiotic and predominant CPB bacteria (Enterobacteriaceae, Lactococcus, Pseudomonas) led to minor and mainly antagonistic effects in survival of larvae infected with B. bassiana. We believe that prolonged mycosis is necessary for successful development of the fungus because such pathogenesis allows the host to activate antibacterial reactions. Conversely, after infection with high concentrations of the fungus, the host's resources are insufficient to fully activate antibacterial defenses, and this situation makes successful development of the fungus impossible.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"600-620"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNA-mediated insect-resistant transgenic rice poses no risk to a non-target parasitoid, Cotesia chilonis, via direct feeding or through its target host. miRNA 介导的抗虫转基因水稻通过直接取食或目标寄主对非目标寄生虫 Cotesia chilonis 不构成风险。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-22 DOI: 10.1111/1744-7917.13415

Cong Dang, Shan Xiao, Fang Wang, Qi Fang, Hongwei Yao, Kang He, Fei Li, Dawei Xue, Gongyin Ye

{"title":"miRNA-mediated insect-resistant transgenic rice poses no risk to a non-target parasitoid, Cotesia chilonis, via direct feeding or through its target host.","authors":"Cong Dang, Shan Xiao, Fang Wang, Qi Fang, Hongwei Yao, Kang He, Fei Li, Dawei Xue, Gongyin Ye","doi":"10.1111/1744-7917.13415","DOIUrl":"10.1111/1744-7917.13415","url":null,"abstract":"MicroRNAs (miRNAs) have started to play an important role in pest control, and novel miRNA-based transgenic insect-resistant plants are now emerging. However, an environmental risk assessment of these novel transgenic plants expressing insect miRNAs must be undertaken before promoting their application. Here, transgenic miR-14 rice, which has high resistance to the rice stem borer Chilo suppressalis, was used as an example for evaluation in this study. Taking the tier 1 risk assessment method in Bacillus thuringiensis (Bt) crops as a reference, the effects of the direct exposure of a non-target parasitoid, Cotesia chilonis, to a high concentration of miRNA were evaluated. The results showed that direct feeding with miR-14 at high concentration had no significant effects on the biological parameters of Co. chilonis, whereas when miR-14 was injected into Ch. suppressalis-parasitized larvae, the development duration of Co. chilonis was significantly affected. In combination with the real conditions of the rice paddy field, it could be inferred that transgenic miR-14 rice has no significant negative effects on the important non-target parasitoid, Co. chilonis. These results will provide a foundation for the establishment of a new safety evaluation system for novel RNAi-based transgenic plants.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"621-630"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABHD5 regulates midgut-specific lipid homeostasis in Bombyx mori. ABHD5调控桑蚕中肠特异性脂质平衡

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-06 DOI: 10.1111/1744-7917.13386

Zhiping Xing, Yuting Zhang, Hongxia Kang, Hui Dong, Dalin Zhu, Yutong Liu, Chenxin Sun, Peilin Guo, Bo Hu, Anjiang Tan

{"title":"ABHD5 regulates midgut-specific lipid homeostasis in Bombyx mori.","authors":"Zhiping Xing, Yuting Zhang, Hongxia Kang, Hui Dong, Dalin Zhu, Yutong Liu, Chenxin Sun, Peilin Guo, Bo Hu, Anjiang Tan","doi":"10.1111/1744-7917.13386","DOIUrl":"10.1111/1744-7917.13386","url":null,"abstract":"Lipids are an important energy source and are utilized as substrates for various physiological processes in insects. Comparative gene identification 58 (CGI-58), also known as α/β hydrolase domain-containing 5 (ABHD5), is a highly conserved and multifunctional gene involved in regulating lipid metabolism and cellular energy balance in many organisms. However, the biological functions of ABHD5 in insects are poorly understood. In the current study, we describe the identification and characterization of the ABHD5 gene in the lepidopteran model insect, Bombyx mori. The tissue expression profile investigated using quantitative reverse transcription polymerase chain reaction (RT-qPCR) reveals that BmABHD5 is widely expressed in all tissues, with particularly high levels found in the midgut and testis. A binary transgenic CRISPR/Cas9 system was employed to conduct a functional analysis of BmABHD5, with the mutation of BmABHD5 leading to the dysregulation of lipid metabolism and excessive lipid accumulation in the larval midgut. Histological and physiological analysis further reveals a significant accumulation of lipid droplets in the midgut of mutant larvae. RNA-seq and RT-qPCR analysis showed that genes related to metabolic pathways were significantly affected by the absence of BmABHD5. Altogether, our data prove that BmABHD5 plays an important role in regulating tissue-specific lipid metabolism in the silkworm midgut.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"425-436"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and phylogenetic analysis of the tetraspanin gene family in lepidopteran insects and expression profiling analysis in Helicoverpa armigera. 鳞翅目昆虫四泛素基因家族的全基因组鉴定和系统进化分析以及 Helicoverpa armigera 的表达谱分析。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-16 DOI: 10.1111/1744-7917.13402

Chenyang Wang, Yinuo Zhang, Fang Guan, Ya-Zhou He, Yidong Wu

{"title":"Genome-wide identification and phylogenetic analysis of the tetraspanin gene family in lepidopteran insects and expression profiling analysis in Helicoverpa armigera.","authors":"Chenyang Wang, Yinuo Zhang, Fang Guan, Ya-Zhou He, Yidong Wu","doi":"10.1111/1744-7917.13402","DOIUrl":"10.1111/1744-7917.13402","url":null,"abstract":"The tetraspanin gene family encodes cell-surface proteins that span the membrane 4 times and play critical roles in a wide range of biological processes across numerous organisms. Recent findings highlight the involvement of a tetraspanin of the lepidopteran pest Helicoverpa armigera in resistance to Bacillus thuringiensis Cry insecticidal proteins, which are extensively used in transgenic crops. Thus, a better understanding of lepidopteran tetraspanins is urgently needed. In the current study, genome scanning in 10 lepidopteran species identified a total of 283 sequences encoding potential tetraspanins. Based on conserved cysteine patterns in the large extracellular loop and their phylogenetic relationships, these tetraspanins were classified into 8 subfamilies (TspA to TspH). Six ancestral introns were identified within lepidopteran tetraspanin genes. Tetraspanins in TspA, TspB, TspC, and TspD subfamilies exhibit highly similar gene organization, while tetraspanins in the remaining 4 subfamilies exhibited variation in intron loss and/or gain during evolution. Analysis of chromosomal distribution revealed a lepidopteran-specific cluster of 10 to 11 tetraspanins, likely formed by tandem duplication events. Selective pressure analysis indicated negative selection across all orthologous groups, with ω values ranging between 0.004 and 0.362. However, positive selection was identified at 18 sites within TspB5, TspC5, TspE3, and TspF10. Furthermore, spatiotemporal expression analysis of H. armigera tetraspanins demonstrated variable expression levels across different developmental stages and tissues, suggesting diverse functions of tetraspanin members in this globally important insect pest. Our findings establish a solid foundation for subsequent functional investigations of tetraspanins in lepidopteran species.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"471-486"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-level genome of the parthenogenetic booklouse Liposcelis bostrychophila reveals high heterozygosity and a nonhomologous chromosome. 孤雌生殖书鼠 Liposcelis bostrychophila 的染色体级基因组显示了高杂合度和非同源染色体。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-06 DOI: 10.1111/1744-7917.13387

Shi-Die Jiang, Yu-Ang Chen, Peng-Yu Guo, Jun Sun, Hua-Yue Feng, Yi Li, Jin-Jun Wang, Dan-Dan Wei

{"title":"Chromosome-level genome of the parthenogenetic booklouse Liposcelis bostrychophila reveals high heterozygosity and a nonhomologous chromosome.","authors":"Shi-Die Jiang, Yu-Ang Chen, Peng-Yu Guo, Jun Sun, Hua-Yue Feng, Yi Li, Jin-Jun Wang, Dan-Dan Wei","doi":"10.1111/1744-7917.13387","DOIUrl":"10.1111/1744-7917.13387","url":null,"abstract":"Psocodean species are emerging as significant sanitary and stored-product pests, posing threats to human health and global food security. Out of an estimated 10 000 species, the whole genome sequences of only 4 species have been published. Genomic resources are crucial for establishing effective pest control and enhancing our understanding of the evolution of psocodean species. In this study, we employed Illumina and PacBio sequencing along with Hi-C scaffolding techniques to generate a chromosome-level genome assembly for the parthenogenetic booklouse Liposcelis bostrychophila. The assembled genome of this booklouse measures 291.67 Mb in length and comprises 9 chromosomes. Notably, the genome of L. bostrychophila exhibits a high level of heterozygosity and features a distinctive nonhomologous chromosome. This heterozygous characteristic of the parthenogenetic booklouse genome may arise from high mutation rates, based on genomic variations analysis across multiple generations. Our analysis revealed significantly expanded gene families, primarily associated with the detoxification and feeding habits of L. bostrychophila. These include integument esterases (ESTs), ATP-binding cassette (ABC) transporter genes and gustatory receptors (GRs). The high-quality genome sequence of L. bostrychophila provides valuable resources for further study on the molecular mechanisms of stress resistance. It enables researchers to identify crucial functional genes and facilitates research on the population genetics, evolution and phylogeny of booklice.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"437-456"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the roles of MAD proteins in the wing dimorphism of Nilaparvata lugens. 分析 MAD 蛋白在 Nilaparvata lugens 翅膀二态性中的作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-03 DOI: 10.1111/1744-7917.13409

Miaomiao Tian, Zeiwei Lu, Jiguang Luo, Huilin Han, Dong Wen, Muhua Zhao, Zhihui Zhu, Hongxia Hua

{"title":"Analysis of the roles of MAD proteins in the wing dimorphism of Nilaparvata lugens.","authors":"Miaomiao Tian, Zeiwei Lu, Jiguang Luo, Huilin Han, Dong Wen, Muhua Zhao, Zhihui Zhu, Hongxia Hua","doi":"10.1111/1744-7917.13409","DOIUrl":"10.1111/1744-7917.13409","url":null,"abstract":"Wing dimorphism in Nilaparvata lugens is controlled by the insulin-like growth factor 1 (IGF-1) signaling - Forkhead transcription factors (IIS-FoxO) pathway. However, the role of this signal in the wing development program remains largely unclear. Here, we identified 2 R-SMAD proteins, NlMAD1 and NlMAD2, in the brown planthopper (BPH) transcriptome, derived from the intrinsic transforming growth factor-β pathway of insect wing development. Both proteins share high sequence similarity and conserved domains. Phylogenetic analysis placed them in the R-SMAD group and revealed related insect orthologs. The expression of Nlmad1 was elevated in the late instar stages of the macropterous BPH strain. Nlmad1 knockdown in nymphs results in malformed wings and reduced wing size in adults, which affects the forewing membrane. By contrast, Nlmad2 expression was relatively consistent across BPH strains and different developmental stages. Nlmad2 knockdown had a milder effect on wing morphology and mainly affected forewing veins and cuticle thickness in the brachypterous strain. NlMAD1 functions downstream of the IIS-FoxO pathway by mediating the FoxO-regulated vestigial transcription and wing morph switching. Inhibiting Nlmad1 partially reversed the long-winged phenotype caused by NlFoxO knockdown. These findings indicate that NlMAD1 and NlMAD2 play distinct roles in regulating wing development and morph differentiation in BPH. Generally, NlMAD1 is a key mediator of the IIS-FoxO pathway in wing morph switching.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"515-529"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extended longevity of termite kings and queens is accompanied by extranuclear localization of telomerase in somatic organs and caste-specific expression of its isoforms. 白蚁王和蚁后的长寿与体细胞器官中端粒酶的核外定位及其同工酶种属特异性表达有关。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-21 DOI: 10.1111/1744-7917.13418

Marie Pangrácová, Jan Křivánek, Markéta Vrchotová, Hana Sehadová, Romana Hadravová, Robert Hanus, Ondřej Lukšan

{"title":"Extended longevity of termite kings and queens is accompanied by extranuclear localization of telomerase in somatic organs and caste-specific expression of its isoforms.","authors":"Marie Pangrácová, Jan Křivánek, Markéta Vrchotová, Hana Sehadová, Romana Hadravová, Robert Hanus, Ondřej Lukšan","doi":"10.1111/1744-7917.13418","DOIUrl":"10.1111/1744-7917.13418","url":null,"abstract":"Kings and queens of termites are endowed with an extraordinary longevity coupled with lifelong fecundity. We recently reported that termite kings and queens display a dramatically increased enzymatic activity and abundance of telomerase in their somatic organs when compared to short-lived workers and soldiers. We hypothesized that this telomerase activation may represent a noncanonical pro-longevity function, independent of its canonical role in telomere maintenance. Here, we explore this avenue and investigate whether the presumed noncanonical role of telomerase may be due to alternative splicing of the catalytic telomerase subunit TERT and whether the subcellular localization of TERT isoforms differs among organs and castes in the termite Prorhinotermes simplex. We empirically confirm the expression of four in silico predicted splice variants (psTERT1-A, psTERT1-B, psTERT2-A, psTERT2-B), defined by N-terminal splicing implicating differential localizations, and C-terminal splicing giving rise to full-length and truncated isoforms. We show that the transcript proportions of the psTERT are caste- and tissue-specific and that the extranuclear full-length isoform TERT1-A is relatively enriched in the soma of neotenic kings and queens compared to their gonads and to the soma of workers. We also show that extranuclear TERT protein quantities are significantly higher in the soma of kings and queens compared to workers, namely due to the cytosolic TERT. Independently, we confirm by microscopy the extranuclear TERT localization in somatic organs. We conclude that the presumed pleiotropic action of telomerase combining the canonical nuclear role in telomere maintenance with extranuclear functions is driven by complex TERT splicing.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"364-384"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria. 通过 RNA 干扰靶向 Syntaxin 1A 可抑制蝗虫的摄食和中肠发育。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-29 DOI: 10.1111/1744-7917.13419

Xiaojian Liu, Ya Gao, Yao Li, Jianzhen Zhang

{"title":"Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria.","authors":"Xiaojian Liu, Ya Gao, Yao Li, Jianzhen Zhang","doi":"10.1111/1744-7917.13419","DOIUrl":"10.1111/1744-7917.13419","url":null,"abstract":"Syntaxin 1A (Syx1A) has diverse and indispensable functions in animals. Previous studies have mainly focused on the roles of Syx1A in Drosophila, and so how Syx1A operates during the development of other insects remains poorly understood. This study investigated whether disrupting LmSyx1A using RNA interference (RNAi) affects the growth and development of Locusta migratoria. LmSyx1A was expressed in all tissues tested, with the highest expression observed in the fat body. After 5th-instar nymphs were injected with double-stranded LmSyx1A (dsLmSyx1A), none of the nymphs were able to molt normally and all eventually died. The silencing of LmSyx1A resulted in the cessation of feeding, body weight loss, and atrophy of the midgut and gastric cecum in locusts. Hematoxylin and eosin (H&E) staining showed that the columnar cells in the midgut were severely damaged, with microvilli defects visible in dsLmSyx1A-injected nymphs. Secretory vesicles were observed with transmission electron microscopy (TEM). In addition, reverse transcription quantitative polymerase chain reaction (RT-qPCR) further indicates that silencing LmSyx1A repressed the expression of genes involved in the insulin/mammalian target of rapamycin (mTOR)-associated nutritional pathway. Taken together, these results suggest that LmSyx1A significantly affects the midgut cell layer of locust nymphs, which was partially associated with the downregulation of the insulin/mTOR-associated nutritional pathway. Thus, we argue that LmSyx1A is a suitable target for developing dsRNA-based biological pesticides for managing L. migratoria.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"385-397"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dynamics of N⁶-methyladenine RNA modification in resistant and susceptible rice varieties responding to rice stem borer damage. 抗性水稻品种和感病水稻品种对水稻二化螟危害的N6-甲基腺嘌呤RNA修饰动态。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-04 DOI: 10.1111/1744-7917.13401

Shuai Li, Xin-Yang Tan, Zhen He, Chen Shen, Ya-Li Li, Lang Qin, Chun-Qing Zhao, Guang-Hua Luo, Ji-Chao Fang, Rui Ji

{"title":"The dynamics of N6-methyladenine RNA modification in resistant and susceptible rice varieties responding to rice stem borer damage.","authors":"Shuai Li, Xin-Yang Tan, Zhen He, Chen Shen, Ya-Li Li, Lang Qin, Chun-Qing Zhao, Guang-Hua Luo, Ji-Chao Fang, Rui Ji","doi":"10.1111/1744-7917.13401","DOIUrl":"10.1111/1744-7917.13401","url":null,"abstract":"N6-methyladenosine (m6A) is the most prevalent modification in cellular RNA which orchestrates diverse physiological and pathological processes during stress response. However, the differential m6A modifications that cope with herbivore stress in resistant and susceptible crop varieties remain unclear. Here, we found that rice stem borer (RSB) larvae grew better on indica rice (e.g., MH63, IR64, Nanjing 11) than on japonica rice varieties (e.g., Nipponbare, Zhonghua 11, Xiushui 11). Then, transcriptome-wide m6A profiling of representative resistant (Nipponbare) and susceptible (MH63) rice varieties were performed using a nanopore direct RNA sequencing approach, to reveal variety-specific m6A modifications against RSB. Upon RSB infestation, m6A methylation occurred in actively expressed genes in Nipponbare and MH63, but the number of methylation sites decreased across rice chromosomes. Integrative analysis showed that m6A methylation levels were closely associated with transcriptional regulation. Genes involved in herbivorous resistance related to mitogen-activated protein kinase, jasmonic acid (JA), and terpenoid biosynthesis pathways, as well as JA-mediated trypsin protease inhibitors, were heavily methylated by m6A, and their expression was more pronounced in RSB-infested Nipponbare than in RSB-infested MH63, which may have contributed to RSB resistance in Nipponbare. Therefore, dynamics of m6A modifications act as the main regulatory strategy for expression of genes involved in plant-insect interactions, which is attributed to differential responses of resistant and susceptible rice varieties to RSB infestation. These findings could contribute to developing molecular breeding strategies for controlling herbivorous pests.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"530-550"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0