Insect Science最新文献_第2页

Diet acts on sexual behavior development in a male moth. 饮食对雄蛾性行为发展的影响

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-14 DOI: 10.1111/1744-7917.13457

Evan Force, Caroline Suray, Christelle Monsempes, Chloé Danis, Gabrielle Bonfils, Stéphane Debernard, Matthieu Dacher

{"title":"Diet acts on sexual behavior development in a male moth.","authors":"Evan Force, Caroline Suray, Christelle Monsempes, Chloé Danis, Gabrielle Bonfils, Stéphane Debernard, Matthieu Dacher","doi":"10.1111/1744-7917.13457","DOIUrl":"https://doi.org/10.1111/1744-7917.13457","url":null,"abstract":"In many animals, drastic changes are observed during sexual maturation characterized by the reproductive system development concomitantly to the sexual behavior ontogenesis. These modifications are under the control of internal and external factors such as food. Sexual maturation requires considerable energetic investment, and diet has been shown to affect reproductive activities in many taxonomic groups, especially in insects and vertebrates. By contrast, diet effects on sexual behavior development remain largely unexplored. To elucidate this aspect, we used the male moth Agrotis ipsilon which undergoes sexual maturation occurring between the third and the fifth day postemergence. During this period, males are sensitive to female sex pheromones and a stereotypical sexual behavior characterized by female-oriented flight takes place. In our study, we compared (1) sex pheromone detection by electroantennography recordings and (2) behavioral response in wind tunnel assays between males fed with different diets found in nature. Compared to standard sucrose diet, males fed with sucrose, fructose, and glucose supplemented with sodium (a mineral element necessary for the locomotor activity in several moths) did not respond better to female sex pheromones but clearly exhibited an earlier behavioral response. Thus, such a diet accelerates the development of sex pheromone-mediated oriented flight, probably by facilitating the central processing of sex pheromone information in male A. ipsilon moths. Our results provide new information on the influence of nutritional intake on the ontogenesis of male sexual behavior in animals.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal concentration of λ-cyhalothrin inhibits insulin-like peptides and leads to reproductive toxicity in Chrysoperla sinica. 亚致死浓度的λ-氯氰菊酯会抑制胰岛素样肽并导致雏菊的生殖毒性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-14 DOI: 10.1111/1744-7917.13463

Nianmeng Wang, Zijian Wang, Siyuan Gong, Yashu Zhang, Chaobin Xue

{"title":"Sublethal concentration of λ-cyhalothrin inhibits insulin-like peptides and leads to reproductive toxicity in Chrysoperla sinica.","authors":"Nianmeng Wang, Zijian Wang, Siyuan Gong, Yashu Zhang, Chaobin Xue","doi":"10.1111/1744-7917.13463","DOIUrl":"https://doi.org/10.1111/1744-7917.13463","url":null,"abstract":"Insulin-like peptides (ILPs) act as crucial reproductive neuropeptides in insects, regulating insect reproduction through the insulin signaling pathway (ISP). Our previous studies have found that the sublethal concentrations (LC1 and LC10) of lambda-cyhalothrin (λCy) could induce severe reproductive toxicity in the lacewing, Chrysoperla sinica (Tjeder), but the toxicological mechanism remains unclear. This study discovered that λCy could inhibit CsILP transcription, leading to a decrease in insulin content and downregulation of C. sinica insulin receptor (CsInR) and C. sinica forkhead box O (CsFOXO) expression in ISP. Interference with CsILP expression resulted in downregulation of C. sinica vitellogenin (CsVg) and decreasing fecundity, while exogenous injection of bovine insulin promoted upregulation of CsVg expression and facilitated reproduction in female adults of C. sinica. Meanwhile, interference with FOXO of ILP downstream transcription factor could lead to downregulation of CsVg, hindering ovarian development and resulting in a decrease in egg production. However, exogenous injection of bovine insulin could remedy the effects caused by FOXO interference. In addition, ILP mediates juvenile hormone and 20-hydroxyecdysone biosynthesis by acting on their synthetic regulatory enzymes and influences the signal transduction of the 2 reproductive endocrine hormones, thereby regulating the reproductive endocrine environment in C. sinica. In conclusion, λCy inhibits CsILP expression, leading to disorder of ISP, leading to the reduced fecundity of C. sinica.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotonin suppresses intraspecific aggression in an agrobiont spider, Pardosa pseudoannulata, without affecting predation on insects. 羟色胺能抑制农蛛（Pardosa pseudoannulata）的种内攻击行为，但不影响对昆虫的捕食。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-09 DOI: 10.1111/1744-7917.13456

Shuchen Dong, Tao Chen, Yunru Chen, Yilin Wang, Yihao Yan, Xuerui Liu, Zewen Liu, Na Yu

{"title":"Serotonin suppresses intraspecific aggression in an agrobiont spider, Pardosa pseudoannulata, without affecting predation on insects.","authors":"Shuchen Dong, Tao Chen, Yunru Chen, Yilin Wang, Yihao Yan, Xuerui Liu, Zewen Liu, Na Yu","doi":"10.1111/1744-7917.13456","DOIUrl":"https://doi.org/10.1111/1744-7917.13456","url":null,"abstract":"Spiders are an abundant group of natural enemies preying on insect pests in agroecosystem. But their potential in biological control has not been fully realized due to difficult mass production. One hindrance is the intense intraspecific aggression in spiders. Neurotransmitters such as serotonin play important roles in modulating aggression. Here, we investigated the regulatory function of serotonin (5-hydroxytryptamine [5-HT]) signaling in the intraspecific aggression in a wandering spider Pardosa pseudoannulata (Araneae, Lycosidae). The aggression was quantified with 5 escalated aggression behaviors as approach, chasing, lunging, boxing, and biting. Virgin (VG) females exhibited higher aggression levels but less 5-HT content than post-reproductive (PR) females. Systemic increase of 5-HT via 5-HT injection decreased aggression, while decrease of 5-HT via RNA interference (RNAi) of the tryptophan hydroxylase gene, increased aggression. The involvement of the four 5-HT receptors were determined via individual or combined RNAi. Co-RNAi of the three 5-HT1 genes increased overall aggression with decreased incidents of approach, chasing, lunging, and increased biting. RNAi of 5-HT1B decreased approach and increased biting, whereas RNAi of 5-HT1A or 5-HT1C did not affect aggression. RNAi of 5-HT7 decreased approach only. Therefore, different 5-HT receptor types contribute to different aspects of the inhibitory effects of 5-HT on aggression and provide several pharmacological targets for manipulating spider aggression. 5-HT injection did not affect spiders' predation on their insect prey, the brown planthopper Nilaparvata lugens. The findings reveal 1 neuronal mechanism regulating intraspecific aggression in spiders and provide an insight in developing aggression suppression strategies for spider mass rearing.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved fungal effector NLS1 suppresses Lepidoptera insect immunity by targeting the host defense protein Hdd11. 保守的真菌效应因子 NLS1 通过靶向宿主防御蛋白 Hdd11 抑制鳞翅目昆虫的免疫力。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-09 DOI: 10.1111/1744-7917.13454

Yulong Wang, Ying Dong, Kexin Liu, Gen Li, Jing Cheng, Yin Cao, Yang Yang, Li Qin, Bo Huang

{"title":"Conserved fungal effector NLS1 suppresses Lepidoptera insect immunity by targeting the host defense protein Hdd11.","authors":"Yulong Wang, Ying Dong, Kexin Liu, Gen Li, Jing Cheng, Yin Cao, Yang Yang, Li Qin, Bo Huang","doi":"10.1111/1744-7917.13454","DOIUrl":"https://doi.org/10.1111/1744-7917.13454","url":null,"abstract":"Entomopathogenic fungi have been widely used as the main mycoinsecticide for controlling agricultural and forest pests. The effector molecules of these mycopathogens have evolved to adapt to their hosts. The role of fungal effectors in evading the host immune system in insects remains mainly unclear. We characterized the widely distributed fungal effector necrosis-inducing-like secreted protein 1 (NLS1) in the entomopathogenic fungus Metarhizium robertsii. Our findings revealed the presence of M. robertsii NLS1 (MrNLS1) in host hemocytes during the early stage of hemocoel infection. MrNLS1 knock down (ΔMrNLS1) reduced fungal pathogenicity during infection and altered the expression of host immune genes. The molecular docking results and the yeast 2-hybrid assay confirmed that MrNLS1 interacts with the host defense protein Hdd11. The phylogenetic analysis indicated that Hdd11 is conserved across a broad range of Lepidoptera species. Knock down of hdd11 in Helicoverpa armigera, Bombyx mori, and Galleria mellonella markedly suppressed their immune responses against M. robertsii. However, no significant difference was observed in the mean lethal time between hdd11-knockdown Lepidoptera species infected with ΔMrNLS1 and those infected with wild-type M. robertsii. Therefore, in Lepidoptera insects, Hdd11 is essential for fungal defense. In conclusion, M. robertsii infects Lepidoptera insects by targeting host Hdd11 through its protein MrNLS1, thereby suppressing the host immune response. Our findings clarify the molecular mechanisms underlying fungal infection pathogenesis.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphis craccivora (Hemiptera: Aphididae) synthesizes juvenile hormone III via a pathway involving epoxidation followed by esterification, potentially providing an epoxidation active site for the synthesis of juvenile hormone SB3. Aphis craccivora（半翅目：蚜科）通过环氧化然后酯化的途径合成幼虫激素 III，这可能为幼虫激素 SB3 的合成提供了一个环氧化活性位点。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-04 DOI: 10.1111/1744-7917.13450

Haolin Li, Xue Kong, Yan Fang, Jiangan Hou, Wenjie Zhang, Yongheng Zhang, Jiguang Wei, Xuesheng Li

{"title":"Aphis craccivora (Hemiptera: Aphididae) synthesizes juvenile hormone III via a pathway involving epoxidation followed by esterification, potentially providing an epoxidation active site for the synthesis of juvenile hormone SB3.","authors":"Haolin Li, Xue Kong, Yan Fang, Jiangan Hou, Wenjie Zhang, Yongheng Zhang, Jiguang Wei, Xuesheng Li","doi":"10.1111/1744-7917.13450","DOIUrl":"https://doi.org/10.1111/1744-7917.13450","url":null,"abstract":"Juvenile hormones (JHs) play a crucial role in regulating development and reproduction in insects. Most insects predominantly synthesize JH III, which typically involves esterification followed by epoxidation, lepidopteran insects use a pathway of epoxidation followed by esterification. Although hemipteran insects have JH III and JH skipped bisepoxide III (JH SB3), the synthesis pathway and key epoxidases remain unclear. This study was conducted on Aphis craccivora, and demonstrated that corpora allata, microsomes, Ac-CYP15C1, and Ac-JHAMT catalyze JH III production in vitro, establishing the pathway of epoxidation followed by esterification. These findings were further confirmed through RNA interference and molecular docking. The presence of JH III and JH SB3 in A. craccivora was identified, and their synthesis pathway was elucidated as follows: Ac-CYP15C1 oxidizes farnesic acid to JH A, followed by methylation to JH III by Ac-JHAMT, possibly providing an epoxidation site on the second carbon for JH SB3. This alteration may significantly contribute to the differentiation and functional diversification of JH types in insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and functional characterization of circulating hemocytes in Tribolium castaneum larvae. 蓖麻幼虫循环血细胞的形态和功能特征。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-03 DOI: 10.1111/1744-7917.13455

Suisui Wang, Shiyuan Miao, Yusi Li, Jianhui Wang, Chengjun Li, Yujie Lu, Bin Li

{"title":"Morphological and functional characterization of circulating hemocytes in Tribolium castaneum larvae.","authors":"Suisui Wang, Shiyuan Miao, Yusi Li, Jianhui Wang, Chengjun Li, Yujie Lu, Bin Li","doi":"10.1111/1744-7917.13455","DOIUrl":"https://doi.org/10.1111/1744-7917.13455","url":null,"abstract":"Hemocytes are pivotal in the immune response of insects against invasive pathogens. However, our knowledge of hemocyte types and their specific function in Tribolium castaneum, an increasingly important Coleoptera model insect in various research fields, remains limited. Presently, a combination of morphological criteria and dye-staining properties were used to characterize hemocyte types from T. castaneum larvae, and 4 distinct types were identified: granulocytes, oenocytoids, plasmatocytes and prohemocytes. Following different immune challenges, the total hemocyte counts declined rapidly in the initial phase (at 2 h), then increased over time (at 4 and 6 h) and eventually returned to the naive state by 24 h post-injection. Notably, the morphology of granulocytes underwent dramatic changes, characterized by an expansion of the surface area and an increased production of pseudopods, and with the number of granulocytes rising significantly through mitotic division. Granulocytes and plasmatocytes, the main hemocyte types in T. castaneum larvae, can phagocytose bacteria or latex beads injected into the larval hemolymph in vivo. Furthermore, these hemocytes participate in the encapsulation and melanization processes in vitro, forming capsules to encapsulate and melanize nickel-nitrilotriacetic acid (Ni-NTA) beads. This study provides the first comprehensive characterization of circulating hemocytes in T. castaneum larvae, offering valuable insights into cell-mediated immunity in response to bacterial infection and the injection of latex beads. These results deepen our understanding of the cellular response mechanisms in T. castaneum larvae and lay a solid foundation for subsequent investigations of the involvement of T. castaneum hemocytes in combating pathogens.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conservation genomics analysis reveals recent population decline and possible causes in bumblebee Bombus opulentus. 保护基因组学分析揭示了大黄蜂（Bombus opulentus）近期的种群数量下降及其可能的原因。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-01 Epub Date: 2024-01-31 DOI: 10.1111/1744-7917.13324

Huiling Sang, Yancan Li, Shuxin Tan, Pu Gao, Bei Wang, Shengnan Guo, Shudong Luo, Cheng Sun

{"title":"Conservation genomics analysis reveals recent population decline and possible causes in bumblebee Bombus opulentus.","authors":"Huiling Sang, Yancan Li, Shuxin Tan, Pu Gao, Bei Wang, Shengnan Guo, Shudong Luo, Cheng Sun","doi":"10.1111/1744-7917.13324","DOIUrl":"10.1111/1744-7917.13324","url":null,"abstract":"Bumblebees are a genus of pollinators (Bombus) that play important roles in natural ecosystem and agricultural production. Several bumblebee species have been recorded as under population decline, and the proportion of species experiencing population decline within subgenus Thoracobombus is higher than average. Bombus opulentus is 1 species in Thoracobombus, but little is known about its recent population dynamics. Here, we employed conservation genomics methods to investigate the population dynamics of B. opulentus during the recent past and identify the likely environmental factors that may cause population decline. Firstly, we placed the scaffold-level of B. opulentus reference genome sequence onto chromosome-level using Hi-C technique. Then, based on this reference genome and whole-genome resequencing data for 51 B. opulentus samples, we reconstructed the population structure and effective population size (Ne) trajectories of B. opulentus and identified genes that were under positive selection. Our results revealed that the collected B. opulentus samples could be divided into 2 populations, and 1 of them experienced a recent population decline; the declining population also exhibited lower genetic diversity and higher inbreeding levels. Genes related to high-temperature tolerance, immune response, and detoxication showed signals of positive selection in the declining population, suggesting that climate warming and pathogen/pesticide exposures may contribute to the decline of this B. opulentus population. Taken together, our study provided insights into the demography of B. opulentus populations and highlighted that populations of the same bumblebee species could have contrasting Ne trajectories and population decline could be caused by a combination of various stressors.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1631-1644"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139650692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

m-Aminophenylacetylene induces maternal care in a predatory spider. 间氨基苯乙炔能诱导捕食性蜘蛛的母性关怀。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-01 Epub Date: 2024-02-04 DOI: 10.1111/1744-7917.13329

Yunru Chen, Na Yu, Shuchen Dong, Guanqun Li, Huiya Pan, Zonglei Guo, Zewen Liu

{"title":"m-Aminophenylacetylene induces maternal care in a predatory spider.","authors":"Yunru Chen, Na Yu, Shuchen Dong, Guanqun Li, Huiya Pan, Zonglei Guo, Zewen Liu","doi":"10.1111/1744-7917.13329","DOIUrl":"10.1111/1744-7917.13329","url":null,"abstract":"Maternal care is critically important for the survival of offspring in various animals. Spiders in the family Lycosidae are known for their hunting ability and maternal care behaviors. Predation on newly hatched spiderlings (pulli) by mother spiders decreases when they come into contact, and they carry the pulli on their dorsal surface. However, the factors inducing maternal care in lycosid spiders have not been elucidated. In this study, we investigated maternal care in Pardosa pseudoannulata (Araneae, Lycosidae) females. We proposed that the physical interaction between pulli and mother spiders induces maternal care via m-aminophenylacetylene (m-A), a novel regulator of maternal care. The presence of pulli on the dorsal abdomen of non-mother spiders suppressed pulli predation and increased the pulli-carrying rate, and the absence of pulli on the mother spiders increased pulli predation and decreased the pulli-carrying rate. The compound m-A was abundant in mother spiders, and it could be induced in non-mother spiders when they carried pulli. The topical application of m-A to non-mother spiders and m-A injection decreased pulli predation and increased the pulli-carrying rate, respectively; these findings indicate that m-A in both internal tissues and the integument is required for the induction of maternal care behavior, and the interaction between pulli and females induces the production of m-A. In-depth study of the regulatory mechanism of maternal care will enhance our understanding of spider biology and behavior.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1569-1578"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139681058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bmo-miR-3351 modulates glutathione content and inhibits BmNPV proliferation by targeting BmGSTe6 in Bombyx mori. Bmo-miR-3351通过靶向BmGSTe6调节谷胱甘肽含量并抑制BmNPV增殖。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-01 Epub Date: 2024-01-22 DOI: 10.1111/1744-7917.13318

Hui-Hua Cao, Wei-Wei Kong, Bing Ling, Zhi-Yi Wang, Ying Zhang, Zhe-Xiao Guo, Shi-Huo Liu, Jia-Ping Xu

{"title":"Bmo-miR-3351 modulates glutathione content and inhibits BmNPV proliferation by targeting BmGSTe6 in Bombyx mori.","authors":"Hui-Hua Cao, Wei-Wei Kong, Bing Ling, Zhi-Yi Wang, Ying Zhang, Zhe-Xiao Guo, Shi-Huo Liu, Jia-Ping Xu","doi":"10.1111/1744-7917.13318","DOIUrl":"10.1111/1744-7917.13318","url":null,"abstract":"MicroRNAs (miRNAs) are small non-coding RNAs that play pivotal roles in the host response to invading pathogens. Among these pathogens, Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main causes of substantial economic losses in sericulture, and there are relatively few studies on the specific functions of miRNAs in the B. mori-BmNPV interaction. Therefore, we conducted transcriptome sequencing to identify differentially expressed (DE) messenger RNAs (mRNAs) and miRNAs in the midgut of 2 B. mori strains (BmNPV-susceptible strain P50 and BmNPV-resistant strain A35) after BmNPV infection. Through correlation analysis of the miRNA and mRNA data, we identified a comprehensive set of 21 miRNAs and 37 predicted target mRNAs. Notably, miR-3351, which has high expression in A35, exhibited remarkable efficacy in suppressing BmNPV proliferation. Additionally, we confirmed that miR-3351 binds to the 3' untranslated region (3' UTR) of B. mori glutathione S-transferase epsilon 6 (BmGSTe6), resulting in its downregulation. Conversely, BmGSTe6 displayed an opposite expression pattern to miR-3351, effectively promoting BmNPV proliferation. Notably, BmGSTe6 levels were positively correlated with glutathione S-transferase activity, consequently influencing intracellular glutathione content in the infected samples. Furthermore, our investigation revealed the protective role of glutathione against BmNPV infection in BmN cells. In summary, miR-3351 modulates glutathione content by downregulating BmGSTe6 to inhibit BmNPV proliferation in B. mori. Our findings enriched the research on the role of B. mori miRNAs in the defense against BmNPV infection, and suggests that the antiviral molecule, glutathione, offers a novel perspective on preventing viral infection in sericulture.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1378-1396"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139520586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of vertebrate complement system by hematophagous arthropods: inhibitory molecules, mechanisms, physiological roles, and applications. 噬血节肢动物对脊椎动物补体系统的抑制：抑制分子、机制、生理作用和应用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-10-01 Epub Date: 2024-01-21 DOI: 10.1111/1744-7917.13317

Mauricio Roberto Vianna Sant'Anna, Adalberto Alves Pereira-Filho, Antonio Ferreira Mendes-Sousa, Naylene Carvalho Sales Silva, Nelder Figueiredo Gontijo, Marcos Horácio Pereira, Leonardo Barbosa Koerich, Grasielle Caldas D'Avila Pessoa, John Andersen, Ricardo Nascimento Araujo

{"title":"Inhibition of vertebrate complement system by hematophagous arthropods: inhibitory molecules, mechanisms, physiological roles, and applications.","authors":"Mauricio Roberto Vianna Sant'Anna, Adalberto Alves Pereira-Filho, Antonio Ferreira Mendes-Sousa, Naylene Carvalho Sales Silva, Nelder Figueiredo Gontijo, Marcos Horácio Pereira, Leonardo Barbosa Koerich, Grasielle Caldas D'Avila Pessoa, John Andersen, Ricardo Nascimento Araujo","doi":"10.1111/1744-7917.13317","DOIUrl":"10.1111/1744-7917.13317","url":null,"abstract":"In arthropods, hematophagy has arisen several times throughout evolution. This specialized feeding behavior offered a highly nutritious diet obtained during blood feeds. On the other hand, blood-sucking arthropods must overcome problems brought on by blood intake and digestion. Host blood complement acts on the bite site and is still active after ingestion, so complement activation is a potential threat to the host's skin feeding environment and to the arthropod gut enterocytes. During evolution, blood-sucking arthropods have selected, either in their saliva or gut, anticomplement molecules that inactivate host blood complement. This review presents an overview of the complement system and discusses the arthropod's salivary and gut anticomplement molecules studied to date, exploring their mechanism of action and other aspects related to the arthropod-host-pathogen interface. The possible therapeutic applications of arthropod's anticomplement molecules are also discussed.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1334-1352"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139512350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0