Insect Science最新文献_第2页

Effects of swine manure mixed with circulating fluidized bed fly ash on black soldier fly (Diptera: Stratiomyidae) larvae and larval frass.

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-05 DOI: 10.1111/1744-7917.13513

Jianwei Hao, Shuang Liu, Mengliang Wang, Wenfeng Hu, Jia Zhao

{"title":"Effects of swine manure mixed with circulating fluidized bed fly ash on black soldier fly (Diptera: Stratiomyidae) larvae and larval frass.","authors":"Jianwei Hao, Shuang Liu, Mengliang Wang, Wenfeng Hu, Jia Zhao","doi":"10.1111/1744-7917.13513","DOIUrl":"https://doi.org/10.1111/1744-7917.13513","url":null,"abstract":"Black soldier fly larvae (BSFL) were reared on mixtures of swine manure and circulating fluidized bed fly ash (CFA) in different ratios. The aim was to evaluate the impacts of insoluble inorganic matter on BSFL and larval frass. The growth performance and nutrient composition of the BSFL were measured under different treatments. The intestinal microbiota structure, morphological characteristics, and total proteolytic activity of the gut were analyzed. The larval frass was tested for nutrients and analyzed using energy-dispersive spectroscopy and scanning electron micrographs. In particular, the surface areas of microparticles from the larval frass (diameter < 0.0074 mm) were measured using Brunauer-Emmett-Teller method. It was found that CFA addition prolonged larval development and reduced the maximum larval weights. The mean larval length, crude protein, and highest larval weight showed negative regression with an increase in the CFA ratio (P < 0.05). Morphological images indicated that physical clogging might be the main influencing factor on larval growth. Moreover, the microbial diversity and complexity in the larval gut increased with CFA addition, but CFA addition had little effect on the composition of dominant phyla or genera (P > 0.05). Finally, the nutrient composition revealed that the frass met the organic fertilizer standard when the CFA addition ratio was less than 7.5%. The optimal addition ratio was 5%, at which the larvae had a more stable and healthier gut environment, but there was less of an effect on larval growth and nutrient composition. Moreover, particles from 5% CFA mixture had the highest surface area.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity of Serratia marcescens strains as biological control agent: Implications for sustainable pest management.

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-05 DOI: 10.1111/1744-7917.13489

Muhammad Rehan Akhtar, Muhammad Younas, Xiaofeng Xia

{"title":"Pathogenicity of Serratia marcescens strains as biological control agent: Implications for sustainable pest management.","authors":"Muhammad Rehan Akhtar, Muhammad Younas, Xiaofeng Xia","doi":"10.1111/1744-7917.13489","DOIUrl":"https://doi.org/10.1111/1744-7917.13489","url":null,"abstract":"The escalating demand for sustainable and eco-friendly pest management strategies has raised interest in harnessing the pathogenic potential of microorganisms. Serratia marcescens, a Gram-negative bacterium, has emerged as a potential biological control agent for sustainable pest management. This review critically examines the history, biology, identification, and pathogenicity of S. marcescens strain with their potential application in pest management. The diverse mechanisms employed by the strain to exert control over pests, including the production of metabolites and the induction of systemic resistance in plants, are examined. The review also summarizes the ecological significance and global distribution of S. marcescens associated with the use of S. marcescens in biological control strategies. Furthermore, the usage efficacy of S. marcescens over other conventional chemicals is discussed. A comprehensive understanding of the pathogenic potential of S. marcescens strains as biological control agents is crucial for developing effective and sustainable pest management strategies. This review consolidates current research advances on S. marcescens, and provides insights into the prospects and challenges of using S. marcescens for integrated pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-11903a modulates CLIPB9-mediated pathogen defense and longevity in Aedes aegypti. miR-11903a 可调节 CLIPB9 介导的埃及伊蚊病原体防御和寿命。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-04 DOI: 10.1111/1744-7917.13512

Yan-Hui Liu, Ya-Nan Yin, Ling-Ling Yu, Meng-He Chang, Qian Han

{"title":"miR-11903a modulates CLIPB9-mediated pathogen defense and longevity in Aedes aegypti.","authors":"Yan-Hui Liu, Ya-Nan Yin, Ling-Ling Yu, Meng-He Chang, Qian Han","doi":"10.1111/1744-7917.13512","DOIUrl":"https://doi.org/10.1111/1744-7917.13512","url":null,"abstract":"Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases (CLIPs). In this study, it was confirmed that microRNA-11903a (miR-11903a) targets Aedes-CLIPB9 (AeCLIPB9) by bioinformatics prediction and dual-luciferase reporter assays. Following intrathoracic injection of miR-11903a agomir and antagomir, Real-time quantitative polymerase chain reaction confirmed that AeCLIPB9 is negatively regulated by miR-11903a. Spatiotemporal expression analysis revealed that miR-11903a is most abundant in 4th instar larvae, followed by pupae and adults, and highly expressed in the wings, head, and midgut of female adults. Following pathogen infection, AeCLIPB9 and miR-11903a exhibited opposite expression trends, indicating their potential roles in mosquito innate immunity. To further investigate the relationship between AeCLIPB9 and miR-11903a, double-strand CLIPB9 was synthesized and RNA interference was performed. Seven-d survival assays revealed that both AeCLIPB9 and miR-11903a were crucial immune factors in fighting pathogens. Finally, longevity assays demonstrated that miR-11903a influenced mosquito lifespan.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome of tropical bed bug Cimex hemipterus (Cimicidae, Hemiptera) reveals tetraspanin expanded in bed bug ancestor. 热带臭虫 Cimex hemipterus（Cimicidae，半翅目）的基因组揭示了臭虫祖先中四角蛋白的扩增。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-06-03 DOI: 10.1111/1744-7917.13388

Sean Tsz Sum Law, Wenyan Nong, Chade Li, Tze Kiu Chong, Ho Yin Yip, Thomas Swale, Siu Wai Chiu, Roger Yat-Nork Chung, Hon-Ming Lam, Samuel Y S Wong, Hung Wong, Jerome H L Hui

{"title":"Genome of tropical bed bug Cimex hemipterus (Cimicidae, Hemiptera) reveals tetraspanin expanded in bed bug ancestor.","authors":"Sean Tsz Sum Law, Wenyan Nong, Chade Li, Tze Kiu Chong, Ho Yin Yip, Thomas Swale, Siu Wai Chiu, Roger Yat-Nork Chung, Hon-Ming Lam, Samuel Y S Wong, Hung Wong, Jerome H L Hui","doi":"10.1111/1744-7917.13388","DOIUrl":"10.1111/1744-7917.13388","url":null,"abstract":"Cimex species are ectoparasites that exclusively feed on warm-blooded animals such as birds and mammals. Three cimicid species are known to be persistent pests for humans, including the tropical bed bug Cimex hemipterus, common bed bug Cimex lectularius, and Eastern bat bug Leptocimex boueti. To date, genomic information is restricted to the common bed bug C. lectularius, which limits understanding their biology and to provide controls of bed bug infestations. Here, a chromosomal-level genome assembly of C. hemipterus (495 Mb [megabase pairs]) contained on 16 pseudochromosomes (scaffold N50 = 34 Mb), together with 9 messenger RNA and small RNA transcriptomes were obtained. In comparison between hemipteran genomes, we found that the tetraspanin superfamily was expanded in the Cimex ancestor. This study provides the first genome assembly for the tropical bed bug C. hemipterus, and offers an unprecedented opportunity to address questions relating to bed bug infestations, as well as genomic evolution to hemipterans more widely.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"42-54"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Let-7 microRNA targets BmCentrin to modulate the development and functionality of the middle silk gland in the silkworm, Bombyx mori. Let-7 microRNA 以 BmCentrin 为靶标，调节家蚕中丝腺的发育和功能。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-29 DOI: 10.1111/1744-7917.13380

Yankun Xu, Na Du, Lili Xu, Lu Zhao, Ting Fan, Tianqi Wei, Qian Pu, Shiping Liu

{"title":"Let-7 microRNA targets BmCentrin to modulate the development and functionality of the middle silk gland in the silkworm, Bombyx mori.","authors":"Yankun Xu, Na Du, Lili Xu, Lu Zhao, Ting Fan, Tianqi Wei, Qian Pu, Shiping Liu","doi":"10.1111/1744-7917.13380","DOIUrl":"10.1111/1744-7917.13380","url":null,"abstract":"The silk gland of the silkworm Bombyx mori serves as a valuable model for investigating the morphological structure and physiological functions of organs. Previous studies have demonstrated the notable regulatory role of let-7 microRNA in the silk gland, but its specific molecular mechanism remains to be elucidated across different segments of this organ. In this study, we further investigated the functional mechanism of let-7 in the middle silk gland (MSG). The MSG of a let-7 knockout strain was analyzed using a combined proteomic and metabolomic technique, revealing the enrichment of differential proteins and metabolites in the DNA synthesis and energy metabolism pathways. BmCentrin was identified as a novel target gene of let-7 in the MSG, and its downregulation inhibited the proliferation of BmN4-SID1 cells, which is exactly opposite to the role of let-7 in these cells. CRISPR/Cas9 genome editing and transgenic technologies were employed to manipulate BmCentrin in the MSG. Knockout of BmCentrin led to severe MSG atrophy, whereas the overexpression of BmCentrin resulted in beaded MSG. Further measurements of these knockout or overexpression strains revealed significant changes in the expression levels of sericin protein genes, the weight of the cocoon and the mechanical properties of the silk. Investigating the biological role of BmCentrin in the silk gland offers valuable insights for elucidating the molecular mechanisms by which let-7 controls silk gland development and silk protein synthesis in the silkworm.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"95-114"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer, Ostrinia furnacalis. 一种具有双重碳水化合物识别结构域的 C 型凝集素在亚洲玉米螟的先天免疫反应中发挥作用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-21 DOI: 10.1111/1744-7917.13382

Er-Tao Li, Jia-Yue Ji, Wei-Jie Kong, Dong-Xu Shen, Cai Li, Chun-Ju An

{"title":"A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer, Ostrinia furnacalis.","authors":"Er-Tao Li, Jia-Yue Ji, Wei-Jie Kong, Dong-Xu Shen, Cai Li, Chun-Ju An","doi":"10.1111/1744-7917.13382","DOIUrl":"10.1111/1744-7917.13382","url":null,"abstract":"C-type lectins (CTLs) act as pattern recognition receptors (PRRs) to initiate the innate immune response in insects. A CTL with dual carbohydrate recognition domains (CRDs) (named immulectin-4 [IML-4]) was selected from the Ostrinia furnacalis transcriptome dataset for functional studies. We cloned the full-length complementary DNA of O. furnacalis IML-4 (OfIML-4). It encodes a 328-residue protein with a Glu-Pro-Asn (EPN) and Gln-Pro-Asp (QPD) motifs in 2 CRDs, respectively. OfIML-4 messenger RNA levels increased significantly upon the bacterial and fungal infection. Recombinant OfIML-4 (rIML-4) and its individual CRDs (rCRD1 and rCRD2) exhibited the binding ability to various microorganisms including Escherichia coli, Micrococcus luteus, Pichia pastoris, and Beauveria bassiana, and the cell wall components including lipopolysaccharide from E. coli, peptidoglycan from M. luteus or Bacillus subtilis, and curdlan from Alcaligenes faecalis. The binding further induced the agglutination of E. coli, M. luteus, and B. bassiana in the presence of calcium, the phagocytosis of Staphylococcus aureus by the hemocytes, in vitro encapsulation and melanization of nickel-nitrilotriacetic acid beads, and a significant increase in phenoloxidase activity of plasma. In addition, rIML-4 significantly enhanced the phagocytosis, nodulation, and resistance of O. furnacalis to B. bassiana. Taken together, our results suggest that OfIML-4 potentially works as a PRR to recognize the invading microorganisms, and functions in the innate immune response in O. furnacalis.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"172-192"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escort: a system for rapid and long-term tracking of multiple insect objects. 护航：快速、长期跟踪多个昆虫目标的系统。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-06-17 DOI: 10.1111/1744-7917.13407

Chengshi Wu, Jin Ge, Bin Han, Hengjing Lan, Xian Zhou, Zhuxi Ge, Weichan Cui, Xiaofeng Liu, Xianhui Wang

引用次数: 0

miR-2940-1 is involved in the circadian regulation of oviposition in Aedes albopictus. miR-2940-1 参与了白纹伊蚊排卵的昼夜节律调节。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-03-31 DOI: 10.1111/1744-7917.13362

Xiaolin Xiao, Ling Kong, Zhensheng Xie, Hongkai Liu, Lijun Cai, Siyu Zhao, Jiayong Zhou, Shuang Liu, Jing Wu, Yiming Wu, Peilin Wu, Anthony A James, Xiao-Guang Chen

{"title":"miR-2940-1 is involved in the circadian regulation of oviposition in Aedes albopictus.","authors":"Xiaolin Xiao, Ling Kong, Zhensheng Xie, Hongkai Liu, Lijun Cai, Siyu Zhao, Jiayong Zhou, Shuang Liu, Jing Wu, Yiming Wu, Peilin Wu, Anthony A James, Xiao-Guang Chen","doi":"10.1111/1744-7917.13362","DOIUrl":"10.1111/1744-7917.13362","url":null,"abstract":"The vast majority of all global species have circadian rhythm cycles that allow them to adapt to natural environments. These regular rhythms are regulated by core clock genes and recent studies have also implicated roles for microRNAs in this regulation. Oviposition is an important circadian behavior in the reproductive cycle of insect vectors of diseases, and little is known about the rhythm or its regulation in mosquitoes. Aedes albopictus is a diurnal mosquito that transmits arboviruses and is the major cause of outbreaks of dengue fever in China. We analyzed the oviposition rhythm patterns of A. albopictus under different light/dark conditions and show that the mosquitoes have an oviposition peak between zeitgeber time 9 (ZT 9) and ZT 12. Furthermore, the antagomir-mediated knockdown of expression of the microRNA miR-2940-1 affected the oviposition rhythm of A. albopictus. These data support the conclusion that miR-2940-1 is involved in the regulation of oviposition rhythm in A. albopictus and provide a foundation for using oviposition rhythms as a new target for vector mosquito control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"69-79"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological immunology: do sexual attraction and immunity trade-off through a desaturase? 生态免疫学：性吸引力和免疫力是否通过去饱和酶进行权衡？

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-20 DOI: 10.1111/1744-7917.13379

Ke Gao, Wout van der Heide, Daphne Muijderman, Sarah Nichols, Carmen Karwal, Peter Kuperus, Astrid T Groot

{"title":"Ecological immunology: do sexual attraction and immunity trade-off through a desaturase?","authors":"Ke Gao, Wout van der Heide, Daphne Muijderman, Sarah Nichols, Carmen Karwal, Peter Kuperus, Astrid T Groot","doi":"10.1111/1744-7917.13379","DOIUrl":"10.1111/1744-7917.13379","url":null,"abstract":"Given the limited availability of resources in nature, sexual attractiveness may trade off with immunocompetence, as the immunocompetence handicap hypothesis (ICHH) posits. In invertebrates, a direct link between trade-offs through hormonal/molecular effectors in sexual signals and immunity has not been found so far. Here, we assessed how variation in sexual signals affected parasite infection in two sex pheromone selected lines of the moth Chloridea virescens: an attractive line with a low ratio of 16:Ald/Z11-16:Ald and an unattractive line with a high ratio. When infecting these lines with an apicomplexan parasite, we found that the attractive Low line was significantly more susceptible to the parasite infection than the unattractive High line. Since the ratio difference between these two lines is determined by a delta-11-desturase, we hypothesized that this desaturase may have a dual role, i.e., in the quality of the sexual signal as well as an involvement in immune response, comparable to testosterone in vertebrates. However, when we used CRISPR/cas9 to knockout delta-11-desturase in the attractive Low line, we found that the pheromonal phenotype did change to that of the High line, but the infection susceptibility did not. Notably, when checking the genomic location of delta-11-desaturase in the C. virescens, we found that mucin is adjacent to delta-11-desaturase. When comparing the mucin sequences in both lines, we found four nonsynonymous SNPs in the coding sequence, as well as intronic variation between the two lines. These differences suggest that genetic hitchhiking may explain the variation in susceptibility to parasitic infection.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"290-300"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P450 gene CYP321A8 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda. P450 基因 CYP6a13 是田间蛙类对杀虫剂产生交叉抗药性的原因。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-02-01 Epub Date: 2024-05-21 DOI: 10.1111/1744-7917.13376

Yumei Chen, Yongjie Cen, Yu Liu, Yanan Peng, Yiguang Lin, Qili Feng, Yong Xiao, Sichun Zheng

{"title":"P450 gene CYP321A8 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda.","authors":"Yumei Chen, Yongjie Cen, Yu Liu, Yanan Peng, Yiguang Lin, Qili Feng, Yong Xiao, Sichun Zheng","doi":"10.1111/1744-7917.13376","DOIUrl":"10.1111/1744-7917.13376","url":null,"abstract":"Continuous and long-term use of traditional and new pesticides can result in cross-resistance among pest populations in different fields. Study on the mechanism of cross-resistance and related genes will help resistance management and field pest control. In this study, the pesticide-resistance mechanism in Spodoptera frugiperda (FAW) was studied with field populations in 3 locations of South China. Field FAW populations were highly resistant to traditional insecticides, chlorpyrifos (organophosphate) and deltamethrin (pyrethroid), and had higher levels of cytochrome P450 activity than a non-resistant laboratory strain. Inhibition of P450 activity by piperonyl butoxide significantly increased the sensitivity of resistant FAW in 3 locations to chlorpyrifos, deltamethrin and chlorantraniliprole (amide), a new type of insecticide, suggesting that P450 detoxification is a critical factor for insecticide resistance in field FAW populations. Transcriptomic analysis indicated that 18 P450 genes were upregulated in the field FAW populations collected in 3 regions and in 2 consecutive years, with CYP321A8, the most significantly upregulated one. Knockdown of CYP321A8 messenger RNA by RNA interference resulted in an increased sensitivity to the 3 tested insecticides in the field FAW. Enzyme activity and molecular docking analyses indicated that CYP321A8 enzyme was able to metabolize the 3 tested insecticides and interact with 8 other types of insecticides, confirming that CYP321A8 is a key cross-resistance gene with a wide range of substrates in the field FAW populations across the different regions and can be used as a biomarker and target for management of FAW insecticide resistance in fields.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"227-242"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0