Insect Science最新文献_第3页

Object use in insects. 昆虫中的物体用途。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-13 DOI: 10.1111/1744-7917.13275

Chao Wen, Cai Wang, Xiaoli Guo, Hongyu Li, Haijun Xiao, Junbao Wen, Shikui Dong

引用次数: 0

Spirotetramat reduces fitness of the spotted-wing Drosophila, Drosophila suzukii. Spirotetramat降低斑点翼果蝇的适应性。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-18 DOI: 10.1111/1744-7917.13283

Jing Yang, Justin Flaven-Pouchon, Yiwen Wang, Bernard Moussian

{"title":"Spirotetramat reduces fitness of the spotted-wing Drosophila, Drosophila suzukii.","authors":"Jing Yang, Justin Flaven-Pouchon, Yiwen Wang, Bernard Moussian","doi":"10.1111/1744-7917.13283","DOIUrl":"10.1111/1744-7917.13283","url":null,"abstract":"With climate change, the spotted-wing Drosophila (SWD, Drosophila suzukii) invades a great number of fruit production regions worldwide. A plethora of insecticides are being applied for management of this pest. As expected, SWD develops resistance against some potent insecticides or is rather insensitive to some others. Therefore, there is an urgent need to identify and characterize alternative insecticides to control SWD populations. Here, we have studied the effects of the orally applied inhibitor of the fatty acid synthesis pathway spirotetramat on SWD fitness with respect to fecundity and surface barrier function at different stages. In our experiments, we applied spirotetramat mixed with baker's yeast and showed that females and males were not repelled by effective concentrations of this insecticide. We found that spirotetramat, by trend, lowers egg numbers laid by fed females. These eggs rapidly desiccate, and only a few larvae hatch. Spirotetramat is lethal to larvae and reduces survival of adult flies under low-humidity conditions. Taken together, based on our data, we propose to use yeast supplemented with spirotetramat and additional SWD-specific attractants in traps in non-crop areas in order to eradicate SWD populations before they infest crop production sites.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excitation of the abdominal ganglion affects the electrophysiological activity of indirect flight muscles of the honeybee Apis mellifera. 腹神经节的兴奋影响蜜蜂间接飞行肌的电生理活动。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-31 DOI: 10.1111/1744-7917.13290

Haojia Ding, Shaoze Yan

{"title":"Excitation of the abdominal ganglion affects the electrophysiological activity of indirect flight muscles of the honeybee Apis mellifera.","authors":"Haojia Ding, Shaoze Yan","doi":"10.1111/1744-7917.13290","DOIUrl":"10.1111/1744-7917.13290","url":null,"abstract":"Our understanding of the nervous tissues that affect the wing flapping of insects mainly focuses on the brain, but wing flapping is a rhythmic movement related to the central pattern generator in the ventral nerve cord. To verify whether the neural activity of the abdominal ganglion of the honeybee (Apis mellifera) affects the flapping-wing flight, we profiled the response characteristics of indirect flight muscles to abdominal ganglion excitation. Strikingly, a change in the neural activity of ganglion 3 or ganglion 4 has a stronger effect on the electrophysiological activity of indirect flight muscles than that of ganglion 5. The electrophysiological activity of vertical indirect flight muscles is affected more by the change in neural activity of the abdominal ganglion than that of lateral indirect flight muscles. Moreover, the change in neural activity of the abdominal ganglion mainly causes the change in the muscular activity of indirect wing muscles, but the activity patterns change relatively little and there is little change in the complicated details. This work improves our understanding of the neuroregulatory mechanisms associated with the flapping-wing flight of honeybees.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71423411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egg-associated secretions from the brown planthopper (Nilaparvata lugens) activate rice immune responses. 褐飞虱(Nilaparvata lugens)的卵相关分泌物激活水稻免疫反应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-27 DOI: 10.1111/1744-7917.13303

Jing Li, Shuai Li, Jing Li, Xinyang Tan, Zhichang Zhao, Lei Jiang, Ary A Hoffmann, Jichao Fang, Rui Ji

{"title":"Egg-associated secretions from the brown planthopper (Nilaparvata lugens) activate rice immune responses.","authors":"Jing Li, Shuai Li, Jing Li, Xinyang Tan, Zhichang Zhao, Lei Jiang, Ary A Hoffmann, Jichao Fang, Rui Ji","doi":"10.1111/1744-7917.13303","DOIUrl":"10.1111/1744-7917.13303","url":null,"abstract":"The brown planthopper (BPH, Nilaparvata lugens) is a notorious sap-sucking insect pest that damages rice (Oryza sativa) plants throughout Asia. During BPH feeding, saliva enters rice plant tissues, whereas during oviposition egg-associated secretions (EAS) are deposited in damaged plant tissue. Dynamic changes in rice to planthopper salivary effectors have been widely reported. However, the effects of EAS from planthopper on rice immunity remains largely unexplored. In this study, we found that both infestation of rice by gravid BPH female adults and treatment with the EAS elicited a strong and rapid accumulation of jasmonic acid (JA), JA-isoleucine, and hydrogen peroxide in rice. EAS enhanced plant defenses not only in rice but also in tobacco, and these impaired the performance of BPH on rice, as well as the performance of aphids and whiteflies on tobacco. High-throughput proteome sequencing of EAS led to 110 proteins being identified and 53 proteins with 2 or more unique peptides being detected. Some proteins from BPH EAS were also found in the salivary proteome from herbivores, suggesting potential evolutionary conservation of effector functions across feeding and oviposition; however, others were only identified in EAS, and these are likely specifically related to oviposition. These findings point to novel proteins affecting interactions between planthoppers and rice during oviposition, providing an additional source of information for effector studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138444565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA let-7 targets BmCDK1 to regulate cell proliferation and endomitosis of silk gland in the silkworm, Bombyx mori. MicroRNA let-7 以 BmCDK1 为靶标，调控家蚕丝腺的细胞增殖和内吸。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-12-05 DOI: 10.1111/1744-7917.13302

Wei Wang, Linshengzhe Ji, Xinyuan Jing, Ping Zhao, Qingyou Xia

{"title":"MicroRNA let-7 targets BmCDK1 to regulate cell proliferation and endomitosis of silk gland in the silkworm, Bombyx mori.","authors":"Wei Wang, Linshengzhe Ji, Xinyuan Jing, Ping Zhao, Qingyou Xia","doi":"10.1111/1744-7917.13302","DOIUrl":"10.1111/1744-7917.13302","url":null,"abstract":"MicroRNAs play critical roles in multiple developmental processes in insects. Our previous study showed that CRISPR/Cas9-mediated knock down of the microRNA let-7 in silkworms increased the size of larvae and silk glands, thereby improving the silk production capacity. In this study, we elucidate the molecular mechanism underlying of let-7 regulates growth. Identification of differentially expressed genes in response to let-7 knock down revealed enrichment of pathways associated with cell proliferation and DNA replication. let-7 dysregulation affected the cell cycle and proliferation of the Bombyx mori cell line BmN. Dual-luciferase and target site mutation assays showed that BmCDK1 is a direct target gene of let-7, with only 1 binding site on its 3'-untranslated region. RNA interference of BmCDK1 inhibited cell proliferation, but this effect was counteracted by co-transfection with let-7 antagomir. Moreover, let-7 knock down induced BmCDK1 expression and promoted cell proliferation in multiple tissues, and further induced endomitosis in the silk gland in vivo. Knock down of BmCDK1 resulted in abnormal formation of a new epidermis, and larval development was arrested at the 2nd or 3rd molt stage. Taken together, our results demonstrated that BmCDK1 is a novel target of let-7 in cell fate determination, possessing potential for improving silk yield in silkworm.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel diagnostic gene region for distinguishing between two pest fruit flies: Bactrocera tryoni (Froggatt) and Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae). 一种区分两种害虫果蝇的新诊断基因区域:特氏小实蝇(Froggatt)和新肱骨小实蝇(Hardy)(双翅目:蝗科)。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-22 DOI: 10.1111/1744-7917.13299

Melissa L Starkie, Elizabeth V Fowler, Alexander M Piper, Xiaocheng Zhu, Pauline Wyatt, David Gopurenko, Matt N Krosch, Francesca Strutt, Karen F Armstrong, Hamish Patrick, Mark K Schutze, Mark J Blacket

{"title":"A novel diagnostic gene region for distinguishing between two pest fruit flies: Bactrocera tryoni (Froggatt) and Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae).","authors":"Melissa L Starkie, Elizabeth V Fowler, Alexander M Piper, Xiaocheng Zhu, Pauline Wyatt, David Gopurenko, Matt N Krosch, Francesca Strutt, Karen F Armstrong, Hamish Patrick, Mark K Schutze, Mark J Blacket","doi":"10.1111/1744-7917.13299","DOIUrl":"10.1111/1744-7917.13299","url":null,"abstract":"Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes, geographic distributions, and host ranges. The need to differentiate between the two species is critical for accurate pest status assessment, management, biosecurity, and maintenance of reference colonies. While morphologically similar, adults may be separated based on subtle characters; however, some characters exhibit intraspecific variability, creating overlap between the two species. Additionally, there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between B. neohumeralis and B. tryoni; therefore, ambiguous samples remain undiagnosed. Here we report the first molecular marker that can consistently distinguish between B. tryoni and B. neohumeralis. Our diagnostic region consists of two adjacent single nucleotide polymorphisms (SNPs) within the pangolin (pan) gene region. We confirmed the genotypes of each species are consistent across their distributional range, then developed a tetra-primer amplification refractory mutation system (ARMS) PCR assay for rapid diagnosis of the species. The assay utilizes four primers in multiplex, with two outer universal primers, and two internal primers: one designed to target two adjacent SNPs (AA) present in B. tryoni and the other targeting adjacent SNPs present in B. neohumeralis (GG). The assay accurately discriminates between the two species, but their SNP genotypes are shared with other nontarget tephritid fruit fly species. Therefore, this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches; maintaining pure colony lines; and in Sterile Insect Technique management responses.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138290871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The neglect of nonnative orthopterans as potential invaders: A call for awareness. 忽视非本地直翅目昆虫作为潜在入侵者：呼吁提高认识。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-10-12 DOI: 10.1111/1744-7917.13277

Anna K Kulessa, Paride Balzani, Ismael Soto, Antonín Kouba, David Renault, Ali Serhan Tarkan, Phillip J Haubrock

引用次数: 0

Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori. 单细胞转录组提供了家蚕胶质细胞扩增的见解。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-20 DOI: 10.1111/1744-7917.13294

Xiaojing Liu, Zhongjie Zhang, Bo Hu, Kai Chen, Ye Yu, Hui Xiang, Anjiang Tan

{"title":"Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori.","authors":"Xiaojing Liu, Zhongjie Zhang, Bo Hu, Kai Chen, Ye Yu, Hui Xiang, Anjiang Tan","doi":"10.1111/1744-7917.13294","DOIUrl":"10.1111/1744-7917.13294","url":null,"abstract":"The diversity of cell types in the brain and how these change during different developmental stages, remains largely unknown. The life cycle of insects is short and goes through 4 distinct stages including embryonic, larval, pupal, and adult stages. During postembryonic life, the larval brain transforms into a mature adult version after metamorphosis. The silkworm, Bombyx mori, is a lepidopteran model insect. Here, we characterized the brain cell repertoire of larval and adult B. mori by obtaining 50 708 single-cell transcriptomes. Seventeen and 12 cell clusters from larval and adult brains were assigned based on marker genes, respectively. Identified cell types include Kenyon cells, optic lobe cells, monoaminergic neurons, surface glia, and astrocyte glia. We further assessed the cell type compositions of larval and adult brains. We found that the transition from larva to adult resulted in great expansion of glial cells. The glial cell accounted for 49.8% of adult midbrain cells. Compared to flies and ants, the mushroom body kenyon cell is insufficient in B. mori, which accounts for 5.4% and 3.6% in larval and adult brains, respectively. Analysis of neuropeptide expression showed that the abundance and specificity of expression varied among individual neuropeptides. Intriguingly, we found that ion transport peptide was specifically expressed in glial cells of larval and adult brains. The cell atlas dataset provides an important resource to explore cell diversity, neural circuits and genetic profiles.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Odorant receptor orthologues from moths display conserved responses to cis-jasmone. 来自飞蛾的气味受体同源物显示对顺式茉莉的保守反应。

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-27 DOI: 10.1111/1744-7917.13296

Xiao-Qing Hou, Zhongqiang Jia, Dan-Dan Zhang, Guirong Wang

{"title":"Odorant receptor orthologues from moths display conserved responses to cis-jasmone.","authors":"Xiao-Qing Hou, Zhongqiang Jia, Dan-Dan Zhang, Guirong Wang","doi":"10.1111/1744-7917.13296","DOIUrl":"10.1111/1744-7917.13296","url":null,"abstract":"In insects, the odorant receptor (OR) multigene family evolves by the birth-and-death evolutionary model, according to which the OR repertoire of each species has undergone specific gene gains and losses depending on their chemical environment, resulting in taxon-specific OR lineage radiations with different sizes in the phylogenetic trees. Despite the general divergence in the gene family across different insect orders, the ORs in moths seem to be genetically conserved across species, clustered into 23 major clades containing multiple orthologous groups with single-copy gene from each species. We hypothesized that ORs in these orthologous groups are tuned to ecologically important compounds and functionally conserved. cis-Jasmone is one of the compounds that not only primes the plant defense of neighboring receiver plants, but also functions as a behavior regulator to various insects. To test our hypothesis, using Xenopus oocyte recordings, we functionally assayed the orthologues of BmorOR56, which has been characterized as a specific receptor for cis-jasmone. Our results showed highly conserved response specificity of the BmorOR56 orthologues, with all receptors within this group exclusively responding to cis-jasmone. This is supported by the dN/dS analysis, showing that strong purifying selection is acting on this group. Moreover, molecular docking showed that the ligand binding pockets of BmorOR56 orthologues to cis-jasmone are similar. Taken together, our results suggest the high conservation of OR for ecologically important compounds across Heterocera.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138444567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient expression of human extracellular superoxide dismutase (rhEcSOD) with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons. 具有抗紫外线损伤活性的人细胞外超氧化物歧化酶(rhEcSOD)在转基因蚕茧中的高效表达

IF 2.9 1区农林科学

Insect Science Pub Date : 2024-08-01 Epub Date: 2023-11-27 DOI: 10.1111/1744-7917.13289

Feng Wang, Ri-Yuan Wang, De-Bin Zhong, Ping Zhao, Qing-You Xia

{"title":"Highly efficient expression of human extracellular superoxide dismutase (rhEcSOD) with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons.","authors":"Feng Wang, Ri-Yuan Wang, De-Bin Zhong, Ping Zhao, Qing-You Xia","doi":"10.1111/1744-7917.13289","DOIUrl":"10.1111/1744-7917.13289","url":null,"abstract":"Extracellular superoxide dismutase (EcSOD) protects tissues from oxidative stress, and thus is considered as a therapeutic agent for many diseases such as atherosclerosis, hypertension, and cancer. However, cost-effective production of bioactive recombinant human EcSOD (rhEcSOD) remains a challenge. Herein, we developed an efficient strategy for producing active rhEcSOD by transgenic silkworms. rhEcSOD was successfully synthesized as homodimers and homotetramers in the middle silk gland and spun into the cocoons with a concentration of 9.48 ± 0.21 mg/g. Purification of rhEcSOD from the cocoons could be conveniently achieved with a purity of 99.50% and a yield of 3.5 ± 0.5 mg/g. Additionally, N-glycosylation at the only site of N89 in rhEcSOD with 10 types were identified. The purified rhEcSOD gained the potent enzymatic activity of 4 162 ± 293 U/mg after Cu/Zn ions incorporation. More importantly, rhEcSOD was capable of penetrating and accumulating in the nuclei of cells to maintain cell morphology and attenuate ultraviolet B-induced cell apoptosis by eliminating reactive oxygen species and inhibiting the C-Jun N-terminal kinase signaling pathway. These results demonstrated that the transgenic silkworm could successfully produce rhEcSOD with enzymatic and biological activities for biomedical applications.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138444566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0