Insect Science最新文献_第3页

Co-phylogeny and biogeography of the myrmecophilous beetle Paussus favieri (Carabidae, Paussinae) and its host ant Pheidole pallidula (Hymenoptera, Myrmicinae). 食蜜瓢虫Paussus favieri（茧蜂科，茧蜂科）及其寄主蚂蚁paidole pallidula（膜翅目，茧蜂科）的共系统发育与生物地理。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-08-06 DOI: 10.1111/1744-7917.70139

Davide Bergamaschi, Wendy Moore, Andrea Di Giulio

{"title":"Co-phylogeny and biogeography of the myrmecophilous beetle Paussus favieri (Carabidae, Paussinae) and its host ant Pheidole pallidula (Hymenoptera, Myrmicinae).","authors":"Davide Bergamaschi, Wendy Moore, Andrea Di Giulio","doi":"10.1111/1744-7917.70139","DOIUrl":"10.1111/1744-7917.70139","url":null,"abstract":"Strict patterns of co-phylogeny have seldom been observed, except among organisms and their symbionts with limited dispersal abilities. In this study, we investigate potential signs of co-phylogeny at the population level between an obligate myrmecophile, the beetle Paussus favieri, and its host ant, Pheidole pallidula. While neither species is physically dependent on the other, as both are fully winged and capable of independent dispersal, Paussus favieri relies entirely on Pheidole pallidula throughout its life cycle. These predatory beetles feed on ants and reproduce in their nests, where they lay eggs and their larvae develop. Therefore, the beetle cannot survive or reproduce without its host, making this an interesting system to explore potential co-phylogenetic patterns. In this paper, we infer population-level phylogenies for both species based on molecular sequence data and apply distance-based and event-based co-phylogenetic methods to search for signs of co-phylogeny, codivergence, or evidence of frequent host population shifts. Molecular phylogenetics reveals significant co-phylogenetic signals, but not phylogenetic congruence, using distance-based methods, as would be expected if the populations of both species shared a similar evolutionary or biogeographic history, without a strict evolutionary dependency. Co-phylogenetic signal without phylogenetic congruence is further explained by event-based methods with a history of codiversification and host population switching, typically occurring among nearby, closely related populations. We discuss the putative mechanisms that might have driven the co-phylogenetic signal between these strictly myrmecophilous beetles and their host ants with particular emphasis on a shared biogeographic scenario within the complex biogeographic history of the Mediterranean Basin.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2210-2226"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesoporous silica nanoparticles enhance the toxicity of chlorantraniliprole to Spodoptera frugiperda by possibly inhibiting energy metabolism and chitin protein synthesis. 介孔二氧化硅纳米颗粒可能通过抑制能量代谢和几丁质蛋白合成来增强氯虫腈对夜蛾的毒性。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-06-24 DOI: 10.1111/1744-7917.70106

Suman Zong, Dejin Xu, Yiping Jiang, Xiaofeng Wang, Keyan Zhu-Salzman, Xin Zhang, Jing Zhao, Liubin Xiao, Leigang Zhang, Guangchun Xu, Aiguo Gu, Hao Hong, Linquan Ge, Yongan Tan

{"title":"Mesoporous silica nanoparticles enhance the toxicity of chlorantraniliprole to Spodoptera frugiperda by possibly inhibiting energy metabolism and chitin protein synthesis.","authors":"Suman Zong, Dejin Xu, Yiping Jiang, Xiaofeng Wang, Keyan Zhu-Salzman, Xin Zhang, Jing Zhao, Liubin Xiao, Leigang Zhang, Guangchun Xu, Aiguo Gu, Hao Hong, Linquan Ge, Yongan Tan","doi":"10.1111/1744-7917.70106","DOIUrl":"10.1111/1744-7917.70106","url":null,"abstract":"Nanosized formulations are an important means to develop effective pesticide molecules against target pests with improved environmental safety. In this study, we constructed a nanodelivery system using mesoporous silica nanoparticles (MSNs) carrying chlorantraniliprole (CLAP). The drug loading rate was determined using liquid chromatography, and CLAP@MSNs were characterized and analyzed. Adhesion was measured by contact angle and surface tension, and UV resistance was assessed. The transport of CLAP@MSNs within plants was observed using confocal fluorescence microscopy. Additionally, larval bioassay experiments and RNA-seq analyses were conducted on CLAP@MSNs. The results indicate that CLAP (35.6% w/w) has been successfully loaded onto MSNs. CLAP@MSNs appeared to have structure and size similar to MSNs. CLAP@MSNs showed effective adhesion to the surface of corn leaves and stems and also resisted to UV photolysis. Bidirectional delivery of fluorescently labeled CLAP@MSNs through vascular bundles in corn. When administered to Spodoptera frugiperda, mortality in CLAP@MSNs-treated larvae increased whereas weight and developmental period decreased significantly compared with larvae treated with CLAP alone. RNA-seq analysis revealed that oral administration of CLAP@MSNs led to the downregulation of genes associated with drug metabolism, energy metabolism, and chitin protein synthesis, thereby inhibiting the growth and development of insects. Interestingly, CLAP@MSNs exerted no harmful effects on growth of plants and development of non-target organisms. Taken together, CLAP@MSNs provide a safe, effective, and economical insecticidal nanopesticide system that potentially further improves the effectiveness of CLAP against lepidopteran pests, contributing to the reduction of pesticide use in pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1993-2011"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient and convenient method for collecting mosquito salivary proteins with high bioactivity and mosquito salivary viruses with high infectivity. 一种高效、简便的蚊虫唾液高生物活性蛋白和蚊虫唾液高传染性病毒的采集方法。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-27 DOI: 10.1111/1744-7917.70136

Zhaoyang Wang, Yan Liang, Dairan Li, Gang Wang, Yibin Zhu, Gong Cheng

{"title":"An efficient and convenient method for collecting mosquito salivary proteins with high bioactivity and mosquito salivary viruses with high infectivity.","authors":"Zhaoyang Wang, Yan Liang, Dairan Li, Gang Wang, Yibin Zhu, Gong Cheng","doi":"10.1111/1744-7917.70136","DOIUrl":"10.1111/1744-7917.70136","url":null,"abstract":"Mosquito salivary proteins (MSPs) play vital roles in facilitating mosquito feeding and mediating mosquito-host interactions. The current standard method for collecting MSPs and mosquito salivary viruses (MSVs) is the forced salivation (FS) method, which is laborious and time-consuming, and yields MSPs with altered composition and reduced bioactivity and MSVs with weakened infectivity, compared to those secreted under physiological conditions. To address these shortcomings, we developed a collecting-by-natural-feeding (CNF) method which empowers convenient and time-saving collection of MSPs and MSVs under physiological conditions. Compared to the FS method, the CNF method enables collection of relatively large quantities of MSPs in a short period of time without any harm to the mosquitoes, allowing for dynamic collection from the same individuals. More importantly, the MSPs and the MSVs collected by the CNF method exhibited a drastically higher bioactivity or infectivity than those collected by the FS method. The CNF method requires no specialized materials or instruments, thus enhancing its general applicability. Collecting sufficient quantities of MSPs with high bioactivity and MSVs with high infectivity has long been a significant challenge. Our novel CNF method may overcome this obstacle, making it highly useful for research on MSPs and vector biology.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1943-1950"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144730189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of Niemann-Pick proteins type C2 as odorant carriers in Neoseiulus barkeri olfactory perception. 尼曼-匹克蛋白C2型在巴氏新小猴嗅觉感知中作为气味载体的功能表征。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-08-05 DOI: 10.1111/1744-7917.70143

Haozhuo Yang, Tiandi Niu, Rongjiang Ma, Dong Xiang, Huai Liu, Hanqiu Chen, Yaying Li

{"title":"Functional characterization of Niemann-Pick proteins type C2 as odorant carriers in Neoseiulus barkeri olfactory perception.","authors":"Haozhuo Yang, Tiandi Niu, Rongjiang Ma, Dong Xiang, Huai Liu, Hanqiu Chen, Yaying Li","doi":"10.1111/1744-7917.70143","DOIUrl":"10.1111/1744-7917.70143","url":null,"abstract":"Arthropods utilize olfactory function to detect informational substances within their natural habitats, thereby facilitating essential life activities such as foraging, mating, and oviposition. Niemann-Pick protein type C2 (NPC2), a putative odorant carrier, has not been fully described in the olfactory perception of predatory mites. In this study, we characterized in detail the olfactory perception functions of NbNPC2-2 and NbNPC2-3 in the important predatory mite Neoseiulus barkeri. The results show that 2 NPC2 proteins, with 6 conserved cysteine sites, are significantly upregulated (by 2.93-fold for NbNPC2-2 and 1.66-fold for NbNPC2-3) with methyl salicylate (MeSA), an important herbivore-induced plant volatile (HIPV). Knocking down both NbNPC2-2 and NbNPC2-3 prevented N. barkeri from preferring pest-infested plants, and reduced their crawling speed to 2.92 and 3.12 mm/s under MeSA treatment. The affinity analysis revealed that NPC2-2 binds to HIPVs β-ionone, MeSA, and ocimene (Ki = 28.55 ± 2.12, 40.73 ± 3.89, and 45.15 ± 2.22, respectively), and NPC2-3 binds to 4-ethylbenzaldehyde (Ki = 51.67 ± 0.23). Molecular docking further confirmed hydrogen bonding interactions between NPC2-2, NPC2-3, and these HIPVs. These results indicated that NPC2-2 and NPC2-3 act as odor carriers involved in the olfactory perception of mites toward HIPVs. This enhances understanding of the molecular mechanism of phytoseiid olfaction.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1915-1926"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of hypomethylation on immune system of Galleria mellonella L. (Lepidoptera: Pyralidae). 低甲基化对黄颡鱼蛾免疫系统的影响。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-22 DOI: 10.1111/1744-7917.70123

Fevzi Uçkan, Rabia Schaeffer, Zülbiye Demirtürk, Ekrem Ergin, Ali Sazci

{"title":"Effects of hypomethylation on immune system of Galleria mellonella L. (Lepidoptera: Pyralidae).","authors":"Fevzi Uçkan, Rabia Schaeffer, Zülbiye Demirtürk, Ekrem Ergin, Ali Sazci","doi":"10.1111/1744-7917.70123","DOIUrl":"10.1111/1744-7917.70123","url":null,"abstract":"Galleria mellonella is becoming increasingly used as a model organism for studying human pathogen infection disease. Therefore, all factors affecting the level of immune response of G. mellonella should be evaluated. One of these factors is DNA methylation, among the most important epigenetic modifications. Firstly, we investigated the presence of CpG dinucleotides and their methylation status of eggs, last instars, pupae, and adults using HpaII, MspI, and ScrFI methylation-sensitive restriction endonucleases. The gel analysis of the restriction pattern of HpaII, MspI, ScrFI, and MspI+ScrFI on G. mellonella genomic DNA showed that all enzymes produced a wide range of smears. The digestion of DNA samples of egg, pupa, and adult resulted in a smear at higher molecular weight for MspI+ScrFI, and these differences were statistically significant. These results confirm the presence of CpG dinucleotides and methylation at the internal and outer cytosines in different developmental stages of G. mellonella. Presence of global DNA methylation, which was by 3%-5% was investigated in various developmental stages of G. mellonella. Then, different levels of DNA methylation in the last instars of G. mellonella were created with the hypomethylation agent, zebularine (0.25-32 mg/mL), and immunity responses were examined. The most effective doses of zebularine, to create a global hypomethylation status in G. mellonella larvae, were 0.25 and 1 mg/mL. The humoral/cellular immune response of G. mellonella larvae became weak at high hypomethylation. These results present the level of DNA methylation during all developmental stages of G. mellonella and how zebularine treatment affects physiological processes.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1797-1816"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of CYP4Gs involved in hydrocarbon formation in the survival, reproduction, and pyrethroid resistance of Triatoma infestans, a major Chagas disease vector. CYP4Gs在恰加斯病主要媒介——斑鼻虫（Triatoma infestans）的生存、繁殖和拟除虫菊酯抗性中参与碳氢化合物形成的作用

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-07 DOI: 10.1111/1744-7917.70100

Nicolás Jesús Nazareth Ifrán, Sergio Javier Mijailovsky, Marianela Santana, Carolina Remón, Juan Roberto Girotti, Gustavo Mario Calderón-Fernández

{"title":"The role of CYP4Gs involved in hydrocarbon formation in the survival, reproduction, and pyrethroid resistance of Triatoma infestans, a major Chagas disease vector.","authors":"Nicolás Jesús Nazareth Ifrán, Sergio Javier Mijailovsky, Marianela Santana, Carolina Remón, Juan Roberto Girotti, Gustavo Mario Calderón-Fernández","doi":"10.1111/1744-7917.70100","DOIUrl":"10.1111/1744-7917.70100","url":null,"abstract":"Very long-chain normal and branched hydrocarbons are essential for insect physiology and ecology. The final metabolic step of hydrocarbon formation involves an oxidative decarbonylation carried out by cytochrome P450 from family 4, subfamily G (CYP4G). Triatoma infestans Klug (Hemiptera: Reduviidae: Triatominae) has two CYP4G genes, named CYP4G106 and CYP4G107. In this study, we have silenced in T. infestans the expression of both genes by RNA interference in order to assess the relevance of hydrocarbons to survival, reproductive physiology, and insecticide susceptibility. Gene knockdown led to a significant decrease (≈ 79%) in total cuticle hydrocarbons, especially normal alkanes. A reduced number of CYP4G-silenced nymphs completed metamorphosis, and adult survival-particularly among females-was markedly diminished. Although mating behavior was not affected by silencing, the reproductive fitness was severely impaired as CYP4G-silenced females laid about 77% fewer eggs per female than control females. Egg hatching was almost completely abolished by CYP4G-silencing. Furthermore, CYP4G gene knockdown increased susceptibility to the insecticide deltamethrin, leading to a 40%-50% higher mortality compared to control insects. These findings highlight the essential role of CYP4G genes in cuticle function, survival, and reproductive fitness and suggest that disruption of hydrocarbon biosynthesis can enhance insecticide efficacy, making it a potential target for control of these insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2012-2024"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal population dynamics and voltinism of the brown marmorated stink bug, Halyomorpha halys in Northern Greece. 希腊北部褐纹臭虫的季节性种群动态和嗜毒性。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-02 DOI: 10.1111/1744-7917.70110

Eleni I Koutsogeorgiou, Theodoros Moysiadis, Emmanuel I Navrozidis, Nikos A Kouloussis, Stefanos S Andreadis

{"title":"Seasonal population dynamics and voltinism of the brown marmorated stink bug, Halyomorpha halys in Northern Greece.","authors":"Eleni I Koutsogeorgiou, Theodoros Moysiadis, Emmanuel I Navrozidis, Nikos A Kouloussis, Stefanos S Andreadis","doi":"10.1111/1744-7917.70110","DOIUrl":"10.1111/1744-7917.70110","url":null,"abstract":"The brown marmorated stink bug, Halyomorpha halys, is an invasive pest posing a significant threat to agricultural crops in various regions, including Northern Greece. This study investigates the seasonal abundance and population dynamics of H. halys in kiwifruit orchards across two prefectures, Imathia and Pieria, over a 3-year period (2021-2023). Monitoring was conducted using pheromone-baited traps to capture mobile life stages from May to October. Our results showcase adult emergence from overwintering sites in April, followed by two peaks in adult captures in late July to mid-August and late September, indicating the presence of two overlapping generations, furtherly corroborated by the corresponding seasonal pattern of L2 nymphs in both regions. Population density and peaks varied regionally, with Imathia consistently exhibiting higher numbers of H. halys compared to Pieria. Environmental factors, such as climatic conditions, likely influenced the timing of adult emergence and diapause termination and the seasonal shifts in population structure. The findings of this study underscore the importance of localized management strategies tailored to the specific ecological conditions of each region to mitigate the impact of H. halys on agricultural production.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2168-2178"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and behavioral bioassays of alarm pheromones from the onion aphid Neotoxoptera formosana. 台湾新弓形虫报警信息素的鉴定及行为生物学分析。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-27 DOI: 10.1111/1744-7917.70130

Yi-Wen Huang, Dong-Hai Xie, Guo-Dong Zhang, Jian-Feng Liu, Zheng-Xi Li, Yao-Guo Qin

{"title":"Identification and behavioral bioassays of alarm pheromones from the onion aphid Neotoxoptera formosana.","authors":"Yi-Wen Huang, Dong-Hai Xie, Guo-Dong Zhang, Jian-Feng Liu, Zheng-Xi Li, Yao-Guo Qin","doi":"10.1111/1744-7917.70130","DOIUrl":"10.1111/1744-7917.70130","url":null,"abstract":"Secreted from aphid cornicles, alarm pheromone is an important part of integrated pest management (IPM). As a destructive pest of economic crops, the onion aphid Neotoxoptera formosana threatens the production and quality of allium crops worldwide. However, the limited understanding of the alarm pheromone has hindered their control through IPM strategies. This work identified the alarm pheromone component from N. formosana and investigated their behavioral activities. The results indicated that 7 major components, α-thujene, sabinene, (+)-limonene, (E)-β-farnesene (EβF), cis-β-farnesene, cis-α-bergamotene, and α-farnesene were identified in the aphid. Among them, EβF and (+)-limonene were also detected in the cornicle of adult with a ratio of 67.8: 1. Quantitative results of the 2 terpenes through developmental stages indicated that amounts of the sesquiterpene EβF increased initially, then decreased from the third instar, and peaked in the adult stage, while the monoterpene (+)-limonene increased progressively with increasing stage. Behavioral bioassays revealed that both compounds exhibited repellent effects at all tested concentrations, with EβF generally displaying significantly higher repellency than (+)-limonene. Notably, the blend of EβF and (+)-limonene at the natural ratio (67.8: 1) showed the highest repellent activity. Pot experiments further confirmed the blend's optimal efficacy against aphids. This work indicated that EβF is the main effective component of the alarm pheromone from N. formosana, but the blend plays a critical role in the repellent behavior. This study enriches understanding of the chemical communication of N. formosana and lays the foundation for the development of green control methods against this aphid.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2071-2080"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144730192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eiger pleiotropically regulates the immunity of Ostrinia furnacalis larvae. 艾格多效性调节玉米螟幼虫免疫功能。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-02 DOI: 10.1111/1744-7917.70108

Honglun Bi, Wenlong Guo, Shiqi Lu, He Zhang, Yulin Liu, Qin Lu, Mengyao Dong, Shuzhong Li, Congjing Feng

{"title":"Eiger pleiotropically regulates the immunity of Ostrinia furnacalis larvae.","authors":"Honglun Bi, Wenlong Guo, Shiqi Lu, He Zhang, Yulin Liu, Qin Lu, Mengyao Dong, Shuzhong Li, Congjing Feng","doi":"10.1111/1744-7917.70108","DOIUrl":"10.1111/1744-7917.70108","url":null,"abstract":"The tumor necrosis factor (TNF) receptor superfamily is a set of essential inflammatory cytokines. In Drosophila, as a homolog of TNF, Eiger was first identified, which is involved in innate immunity signaling pathways. However, in Lepidoptera, the functions of Eiger and its molecular regulatory mechanisms on immune responses are unknown. In this study, Eiger was cloned from Ostrinia furnacalis. OfEiger has a conserved structural domain of the TNF family. The expression of OfEiger increased with the age of O. furnacalis larvae, and was the highest in the hemocytes. After O. furnacalis larvae were injected with Pseudomonas aeruginosa or Micrococcus luteus, the expression of OfEiger was significantly upregulated. The expression levels of several genes in the immune pathways of O. furnacalis larvae were downregulated at 60 h post-injection of double-stranded Eiger (dsEiger), including IMD, Toll, nitric oxide (NO), and mitogen-activated protein kinase (MAPK) signaling pathways. Moreover, the expression levels of the 4 antimicrobial peptides (AMPs), OfLebocin, OfAttacin, OfGloverin, and OfMoricin, were downregulated. In addition, the phagocytosis of Escherichia coli by hemocytes was reduced in O. furnacalis larvae after dsEiger injection. Injections of P. aeruginosa or M. luteus into O. furnacalis larvae following OfEiger RNA interference reduced the larvae's survival rate and increased the expression levels of OfMyD88 and OfJNK. Still, they suppressed the expression of OfNOS1 and AMP genes and inhibited phenoloxidase (PO) activity in O. furnacalis larvae. In conclusion, OfEiger is a vital insect immune regulator, which synergistically regulates IMD, Toll, NO, and MAPK signaling pathways and adjusts PO activity, cellular immunity, AMPs, and other effectors.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1817-1833"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term rearing experiences influence parasitism ability of an ectoparasitoid wasp. 长期饲养对寄生蜂的寄生能力有影响。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-08 DOI: 10.1111/1744-7917.70115

Shumei Pan, Chunxia Wang, Jing Su, Xiangqian Huang, Shiyao Liu, Haodong Hao, Xiuju Yu, Hongxia Li, Jianghua Sun, Li Li, Zhudong Liu

{"title":"Long-term rearing experiences influence parasitism ability of an ectoparasitoid wasp.","authors":"Shumei Pan, Chunxia Wang, Jing Su, Xiangqian Huang, Shiyao Liu, Haodong Hao, Xiuju Yu, Hongxia Li, Jianghua Sun, Li Li, Zhudong Liu","doi":"10.1111/1744-7917.70115","DOIUrl":"10.1111/1744-7917.70115","url":null,"abstract":"Parasitoid wasps face increased mortality when paralyzing resistant hosts, involving host adaptation and biocontrol. This study investigates the plasticity of host adaptation in the ectoparasitoid Sclerodermus guani by examining how long-term rearing on hosts of differing resistance influences parasitism performance. Two distinct wasp lineages were established: the stressful Ma lineage, continuously reared on the highly resistant Monochamus alternatus (Ma) larvae, and the susceptible Sp lineage, continuously reared on the non-resistant Saperda populnea (Sp) larvae. Host preference assays revealed that Ma wasps exhibited a significant preference for Ma larvae, while Sp wasps favored Sp larvae. Although Ma wasps experienced higher mortality and a lower population increase index when reared on Ma larvae compared to the performance of Sp wasps on Sp larvae, they demonstrated superior parasitism efficiency when tested against resistant hosts. Specifically, Ma wasps had significantly lower mortality rates and higher population increase index than Sp wasps when both were tested on Ma larvae. In dual-parasitism trials, where 1 Ma wasp and 1 Sp wasp were introduced to the same Ma larvae, and in group trials involving 3 wasps from each lineage on the novel, high-resistant host Aromia bungii, the Ma wasps outperformed Sp wasps in paralysis success. Behavioral assays further indicated that Ma wasps invested more time in accessing host resistance but were quicker in locating hosts compared to Sp wasps. This research provides valuable insights for improving the design of mass rearing strategies to maximize the effectiveness of biological control agents in integrated pest management programs.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2123-2136"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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