Insect Science最新文献_第3页

Functional characterization of Niemann-Pick proteins type C2 as odorant carriers in Neoseiulus barkeri olfactory perception. 尼曼-匹克蛋白C2型在巴氏新小猴嗅觉感知中作为气味载体的功能表征。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-05 DOI: 10.1111/1744-7917.70143

Haozhuo Yang, Tiandi Niu, Rongjiang Ma, Dong Xiang, Huai Liu, Hanqiu Chen, Yaying Li

{"title":"Functional characterization of Niemann-Pick proteins type C2 as odorant carriers in Neoseiulus barkeri olfactory perception.","authors":"Haozhuo Yang, Tiandi Niu, Rongjiang Ma, Dong Xiang, Huai Liu, Hanqiu Chen, Yaying Li","doi":"10.1111/1744-7917.70143","DOIUrl":"https://doi.org/10.1111/1744-7917.70143","url":null,"abstract":"Arthropods utilize olfactory function to detect informational substances within their natural habitats, thereby facilitating essential life activities such as foraging, mating, and oviposition. Niemann-Pick protein type C2 (NPC2), a putative odorant carrier, has not been fully described in the olfactory perception of predatory mites. In this study, we characterized in detail the olfactory perception functions of NbNPC2-2 and NbNPC2-3 in the important predatory mite Neoseiulus barkeri. The results show that 2 NPC2 proteins, with 6 conserved cysteine sites, are significantly upregulated (by 2.93-fold for NbNPC2-2 and 1.66-fold for NbNPC2-3) with methyl salicylate (MeSA), an important herbivore-induced plant volatile (HIPV). Knocking down both NbNPC2-2 and NbNPC2-3 prevented N. barkeri from preferring pest-infested plants, and reduced their crawling speed to 2.92 and 3.12 mm/s under MeSA treatment. The affinity analysis revealed that NPC2-2 binds to HIPVs β-ionone, MeSA, and ocimene (Ki = 28.55 ± 2.12, 40.73 ± 3.89, and 45.15 ± 2.22, respectively), and NPC2-3 binds to 4-ethylbenzaldehyde (Ki = 51.67 ± 0.23). Molecular docking further confirmed hydrogen bonding interactions between NPC2-2, NPC2-3, and these HIPVs. These results indicated that NPC2-2 and NPC2-3 act as odor carriers involved in the olfactory perception of mites toward HIPVs. This enhances understanding of the molecular mechanism of phytoseiid olfaction.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-nucleus RNA sequencing reveals midgut cellular heterogeneity and transcriptional profiles in Bombyx mori cytoplasmic polyhedrosis virus infection. 单核 RNA 测序揭示了中肠细胞的异质性和多角体病毒感染的转录特征。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-11-10 DOI: 10.1111/1744-7917.13464

Shigang Fei, Mian Muhammad Awais, Jinglei Zou, Junming Xia, Yeyuan Wang, Yibing Kong, Min Feng, Jingchen Sun

{"title":"Single-nucleus RNA sequencing reveals midgut cellular heterogeneity and transcriptional profiles in Bombyx mori cytoplasmic polyhedrosis virus infection.","authors":"Shigang Fei, Mian Muhammad Awais, Jinglei Zou, Junming Xia, Yeyuan Wang, Yibing Kong, Min Feng, Jingchen Sun","doi":"10.1111/1744-7917.13464","DOIUrl":"10.1111/1744-7917.13464","url":null,"abstract":"The gut is not only used by insects as an organ for the digestion of food and absorption of nutrients but also as an important barrier against the invasion and proliferation of pathogenic microorganisms. Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), an insect-specific virus, predominantly colonizes the midgut epithelial cells of the silkworm, thereby jeopardizing its normal growth. However, there is limited knowledge of the cellular immune responses to viral infection and whether the infection is promoted or inhibited by different types of cells in the silkworm midgut. In this study, we used single-nucleus RNA sequencing to identify representative enteroendocrine cells, enterocytes, and muscle cell types in the silkworm midgut. In addition, by analyzing the transcriptional profiles of various subpopulations in the infected and uninfected groups, we found that BmCPV infection suppresses the response of the antiviral pathways and induces the expression of BmHSP70, which plays a role in promoting BmCPV replication. However, certain immune genes in the midgut of the silkworm, such as BmLebocin3, were induced upon viral infection, and downregulation of BmLEB3 using RNA interference promoted BmCPV replication in the midgut of B. mori. These results suggest that viral immune evasion and active host resistance coexist in BmCPV-infected silkworms. We reveal the richness of cellular diversity in the midgut of B. mori larvae by single-nucleus RNA sequencing analysis and provide new insights into the complex interactions between the host and the virus at the single-cell level.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1141-1154"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue-specific alternative splicing and the functional differentiation of LmLPMO15-1 in Locusta migratoria. 组织特异性替代剪接与蝗虫中 LmLPMO15-1 的功能分化。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-11-14 DOI: 10.1111/1744-7917.13469

Lin Kong, Huiying Hu, Pengfei Li, Mingbo Qu

{"title":"Tissue-specific alternative splicing and the functional differentiation of LmLPMO15-1 in Locusta migratoria.","authors":"Lin Kong, Huiying Hu, Pengfei Li, Mingbo Qu","doi":"10.1111/1744-7917.13469","DOIUrl":"10.1111/1744-7917.13469","url":null,"abstract":"Insect lytic polysaccharide monooxygenases (LPMO15s) are newly discovered copper-dependent enzymes that promote chitin degradation in insect through oxidative cleavage of glycosidic bonds. They are potential pesticide targets due to their critical role for chitin turnover in the integument, trachea, and peritrophic matrix of the midgut during insect molting. However, the knowledge about whether and how LPMO15s participate in chitin turnover in other tissues is still insufficient. Here, using the orthopteran pest Locusta migratoria as a model, a novel alternative splicing site of LmLPMO15-1 was discovered and it produces 2 variants, LmLPMO15-1a and LmLPMO15-1b. The transcripts of LmLPMO15-1a and LmLPMO15-1b were specifically expressed in the trachea and foregut, respectively. RNA interference targeting LmLPMO15-1 (a common fragment shared by both LmLPMO15-1a and LmLPMO15-1b), a specific region of LmLPMO15-1a or LmLPMO15-1b all significantly reduced survival rate of nymphs and induced lethal phenotypes with developmental stasis or molt failure. Ultrastructure analysis demonstrated that LmLPMO15-1b was specifically involved in foregut old cuticle degradation, while LmLPMO15-1a was exclusively responsible for the degradation of the tracheal old cuticle. This study revealed LmLPMO15-1 achieved tissue-specific functional differentiation through alternative splicing, and proved the significance of the spliced variants during insect growth and development. It provides new strategies for pest control targeting LPMO15-1.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1241-1255"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-targets cleavage of BmNPV genome through genome-wide repeat sequence using CRISPR/Cas9 antiviral system. 利用 CRISPR/Cas9 抗病毒系统通过全基因组重复序列对 BmNPV 基因组进行多靶点切割。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-20 DOI: 10.1111/1744-7917.13462

Yujia Liu, Xu Yang, Ping Wu, Xijie Guo, Zulian Liu, Yongping Huang, Xia Xu

{"title":"Multi-targets cleavage of BmNPV genome through genome-wide repeat sequence using CRISPR/Cas9 antiviral system.","authors":"Yujia Liu, Xu Yang, Ping Wu, Xijie Guo, Zulian Liu, Yongping Huang, Xia Xu","doi":"10.1111/1744-7917.13462","DOIUrl":"10.1111/1744-7917.13462","url":null,"abstract":"The escalating severity of Bombyx mori nuclear polyhedrosis virus (BmNPV) infections poses significant challenges to the silkworm industry, especially when massive production shifts occur from the eastern regions to western regions with lower labor costs. Education and experience levels are different and disease control is badly needed. To solve the problems, we have developed an innovative CRISPR/Cas9 system specifically targeting BmNPV to enhance viral resistance. For the system, we selected BmNPV genes linked to virus replication and proliferation as targets, designing 2 sites for each gene. Mutating the target sequence renders the system incapable of efficiently cleaving the virus genome, hence decreasing cleavage efficiency. We conducted a search for \"NGG\" or \"CCN\" target sequences in the BmNPV genome, excluding non-recurring and potential targets in the B. mori genome. We successfully identified 2 distinct target sequences in the BmNPV genome-one being repeated 12 times and the other three times. These sequences lead to fragmentation of virus genome into multiple large segments that are difficult to repair. Transgenic silkworms demonstrate robust resistance to viruses, significantly boosting their survival rates compared with wild-type silkworms under various virus infection concentrations. Our system efficiently targets dozens of viral genomes with just 2 sequences, minimizing transposable elements while ensuring cutting effectiveness. This marks a pioneering advancement by using repetitive elements within the virus genome for targeted CRISPR cleavage, aiming for antiviral effects through genome fragmentation rather than disrupting essential viral genes. Our research introduces innovative concepts to CRISPR antiviral investigations and shows promise for the practical application of gene editing in industrial silkworm strains.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1174-1184"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miRNA-mRNA modules enhance juvenile hormone biosynthesis for insect vitellogenesis and egg production. miRNA-mRNA 模块增强了昆虫卵黄发生和产卵过程中幼年激素的生物合成。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-30 DOI: 10.1111/1744-7917.13451

Wanwan Li, Mingzhi Liu, Zitong Zhuang, Lulu Gao, Jiasheng Song, Shutang Zhou

{"title":"The miRNA-mRNA modules enhance juvenile hormone biosynthesis for insect vitellogenesis and egg production.","authors":"Wanwan Li, Mingzhi Liu, Zitong Zhuang, Lulu Gao, Jiasheng Song, Shutang Zhou","doi":"10.1111/1744-7917.13451","DOIUrl":"10.1111/1744-7917.13451","url":null,"abstract":"In addition to preventing precocious larval metamorphosis, juvenile hormone (JH), synthesized in corpora allata (CA), is known to stimulate female reproduction of insects. JH titer is extremely low or absent during metamorphosis, but thereafter rapidly increases in the previtellogenic stage and rises to a peak in the vitellogenic phase. However, the mechanisms underlying the biosynthesis of high levels of JH in adults remain unclear. We found in this study that 12 genes involved in JH synthesis pathway were highly expressed in the CA of adult locusts. By transcriptome analysis and quantitative real-time - polymerase chain reaction validation, a total of 106 evolutionary conserved micro RNAs (miRNAs) and 163 species-specific miRNAs were identified in locust CA. Dual-luciferase assay revealed that 17 miRNAs bound to 10 JH synthesis genes (JHSGs) and downregulated their expression. These miRNAs were expressed in low levels during vitellogenic stage, which was oppositive from that of targeting JHSGs. Six miRNAs including miR-971-3p, miR-31a, miR-9-5p, miR-1-3p, miR-315, and miR-282 were selected for function study. Co-application of agomiRs resulted in significantly decreased levels of targeting JHSGs, accompanied by significantly reduced vitellogenin expression as well as arrested ovarian development. The data suggest that multiple miRNAs expressed synchronously at low levels in the vitellogenic phase, thereby ensuring the high levels of JHSG expression to facilitate JH biosynthesis required for JH-dependent female reproduction. The findings provide important information for deciphering miRNA-messenger RNA modules for JH biosynthesis as well as JH regulation of insect metamorphosis and reproduction.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1227-1240"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gallic acid enhances honeybee larvae resistance to Paenibacillus larvae infections: Insights from molecular docking, bacterial community modulation, and whole-genome sequencing. 没食子酸增强蜜蜂幼虫对芽孢杆菌幼虫感染的抵抗力：来自分子对接、细菌群落调节和全基因组测序的见解。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2025-02-25 DOI: 10.1111/1744-7917.70000

Man-Hong Ye, Yin-Hong Jiang, Xiao-Yuan Li, Qian-Nan Han, Chuang Meng, Feng Ji, Bin Zhou

{"title":"Gallic acid enhances honeybee larvae resistance to Paenibacillus larvae infections: Insights from molecular docking, bacterial community modulation, and whole-genome sequencing.","authors":"Man-Hong Ye, Yin-Hong Jiang, Xiao-Yuan Li, Qian-Nan Han, Chuang Meng, Feng Ji, Bin Zhou","doi":"10.1111/1744-7917.70000","DOIUrl":"10.1111/1744-7917.70000","url":null,"abstract":"American foulbrood (AFB) disease, caused by the bacterium Paenibacillus larvae, is a devastating disease affecting honeybee (Apis mellifera L.) populations worldwide. Commonly treated with antibiotics, which have negative impacts on both honeybees and the environment, there is an urgent need for alternatives in AFB control. This study aimed to investigate the effects of gallic acid (GA) on honeybee larvae challenged with P. larvae spores and explore its modulation of larval microbiota. Our results demonstrated that in the presence of P. larvae spores, coadministration of 125 µg/mL GA significantly increased the survival rate and body weight of honeybee larvae. Molecular docking analyses revealed that GA competitively binds to spore germination proteins YndE and GerM, with affinities comparable to L-tyrosine and stronger than uric acid, respectively, suggesting interference with P. larvae spore germination. 16S rRNA gene amplicon sequencing revealed that GA treatment augmented bacterial diversity and enriched lactic acid bacteria (LAB) in honeybee larvae. Whole-genome sequencing of 2 LAB strains, Apilactobacillus kunkeei GL-2 and Enterococcus faecium GL-6, isolated from GA-treated larvae, unveiled their potential to produce antimicrobial secondary metabolites and bacteriocins, which may contribute to their competitive advantages against P. larvae. Notably, the E. faecium GL-6 strain possessed genes encoding gallate decarboxylase, enabling GA utilization, and 2 putative bacteriocinogenic genetic clusters for enterolysin A and enterocin L50 a/b. These findings suggest that GA and the GL-6 strain hold potential as preventive measures against AFB disease in honeybees through modulation of gut microbiota and competitive inhibition of P. larvae.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1380-1402"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotonin suppresses intraspecific aggression in an agrobiont spider, Pardosa pseudoannulata, without affecting predation on insects. 羟色胺能抑制农蛛（Pardosa pseudoannulata）的种内攻击行为，但不影响对昆虫的捕食。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-10-09 DOI: 10.1111/1744-7917.13456

Shuchen Dong, Tao Chen, Yunru Chen, Yilin Wang, Yihao Yan, Xuerui Liu, Zewen Liu, Na Yu

{"title":"Serotonin suppresses intraspecific aggression in an agrobiont spider, Pardosa pseudoannulata, without affecting predation on insects.","authors":"Shuchen Dong, Tao Chen, Yunru Chen, Yilin Wang, Yihao Yan, Xuerui Liu, Zewen Liu, Na Yu","doi":"10.1111/1744-7917.13456","DOIUrl":"10.1111/1744-7917.13456","url":null,"abstract":"Spiders are an abundant group of natural enemies preying on insect pests in agroecosystem. But their potential in biological control has not been fully realized due to difficult mass production. One hindrance is the intense intraspecific aggression in spiders. Neurotransmitters such as serotonin play important roles in modulating aggression. Here, we investigated the regulatory function of serotonin (5-hydroxytryptamine [5-HT]) signaling in the intraspecific aggression in a wandering spider Pardosa pseudoannulata (Araneae, Lycosidae). The aggression was quantified with 5 escalated aggression behaviors as approach, chasing, lunging, boxing, and biting. Virgin (VG) females exhibited higher aggression levels but less 5-HT content than post-reproductive (PR) females. Systemic increase of 5-HT via 5-HT injection decreased aggression, while decrease of 5-HT via RNA interference (RNAi) of the tryptophan hydroxylase gene, increased aggression. The involvement of the four 5-HT receptors were determined via individual or combined RNAi. Co-RNAi of the three 5-HT1 genes increased overall aggression with decreased incidents of approach, chasing, lunging, and increased biting. RNAi of 5-HT1B decreased approach and increased biting, whereas RNAi of 5-HT1A or 5-HT1C did not affect aggression. RNAi of 5-HT7 decreased approach only. Therefore, different 5-HT receptor types contribute to different aspects of the inhibitory effects of 5-HT on aggression and provide several pharmacological targets for manipulating spider aggression. 5-HT injection did not affect spiders' predation on their insect prey, the brown planthopper Nilaparvata lugens. The findings reveal 1 neuronal mechanism regulating intraspecific aggression in spiders and provide an insight in developing aggression suppression strategies for spider mass rearing.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1427-1438"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori. BmEL-2通过调控BmAGPATγ和BmFAF2的表达来促进桑蚕体内甘油三酯的代谢。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-15 DOI: 10.1111/1744-7917.13446

Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun

{"title":"BmEL-2 promotes triglyceride metabolism by regulating BmAGPATγ and BmFAF2 expression in Bombyx mori.","authors":"Da Ma, Si Zhou, Jiayuan Shi, Ying Gu, Sheng Qin, Muwang Li, Xia Sun","doi":"10.1111/1744-7917.13446","DOIUrl":"10.1111/1744-7917.13446","url":null,"abstract":"Bombyx mori ELAV-like-1 (BmEL-1) and B. mori ELAV-like-2 (BmEL-2) are 2 members of the ELAV-like family of RNA-binding proteins. Mutations in Bmel-1 and Bmel-2 resulted in 5.8% and 28.5% decreases in larval weight on the 3rd day of the 5th instar larva (L5D3), respectively. Triglycerides (TG) are the most important energy resource and are the main component of neutral fat (NF) in animals. To investigate the role of Bmelav-like genes in the synthesis and decomposition of TG, transcriptomic, and metabolic analyses were performed on the whole bodies on the 1st day of the 2nd instar larvae (L2D1) and on fat bodies on L5D3 of Bmel-1- and Bmel-2- mutants, respectively. As compared with the control silkworm, differentially expressed genes generated in both mutants were mainly enriched in lysine degradation, fatty acid (FA) metabolism, and unsaturated FAs biosynthesis. The diglyceride and phosphatide contents were significantly lower in Bmel-1- and Bmel-2- fat bodies than those of the control group. Consistently, the NF content of both mutants' fat bodies were reduced by 50% and 60%, respectively. BmEL-2 positively regulates BmAGPATγ (B. mori 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, LOC101741736) and BmFaF2 (B. mori fatty acid synthetase-associated factor 2, LOC101739090) expression by binding to the specific regions of their 3' untranslated regions in BmN cells. This study suggests that BmEL-2 may be an important regulator of BmAGPATγ and BmFAF2 expression and thereby participates in TG metabolism in the silkworm fat body.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1256-1268"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AMPK regulates HIF-1α to induce pupal diapause in the cotton bollworm, Helicoverpa armigera. AMPK调控HIF-1α诱导棉铃虫蛹滞育。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2025-05-06 DOI: 10.1111/1744-7917.70068

Zhiren Su, Songshan Jiang, Wei-Hua Xu

{"title":"AMPK regulates HIF-1α to induce pupal diapause in the cotton bollworm, Helicoverpa armigera.","authors":"Zhiren Su, Songshan Jiang, Wei-Hua Xu","doi":"10.1111/1744-7917.70068","DOIUrl":"10.1111/1744-7917.70068","url":null,"abstract":"Diapause is an adaptive strategy employed by insects to endure adverse environmental conditions and is characterized by reduced metabolic activity, primarily due to a decreased respiratory rate. AMP-activated protein kinase (AMPK) serves as an intracellular energy regulator, modulating energy metabolism in response to metabolic fluctuations. However, its role in pupal diapause of the cotton bollworm, Helicoverpa armigera, remains unclear. In this study, we found that AMPK and its active form, P-AMPK, are highly expressed in diapause-destined pupae. Furthermore, activation of AMPK delayed the development of nondiapause-destined pupae, suggesting a critical role for AMPK in the regulation of pupal diapause in H. armigera. Manipulating AMPK activity in H. armigera epidermal (HaEpi) cells and pupae significantly influenced the expression of hypoxia-inducible factor-1α (HIF-1α), which our laboratory previously reported as a key inducer of pupal diapause through the reduction of mitochondrial activity in H. armigera. Histone deacetylase 4 (HDAC4), a shuttle protein phosphorylated by AMPK which translocates between the cytoplasm and the nucleus, was found to exhibit significantly higher expression in diapause-destined pupal brains compared to their nondiapause counterparts. AMPK in both HaEpi cells and pupae positively regulated the protein levels of P-HDAC4 by binding to the HDAC4 promoter. Additionally, HDAC4 was shown to enhance HIF-1α expression in diapause-destined individuals. HDAC4 binds to and deacetylates heat shock protein 70 (HSP70), and reduced acetylation of HSP70 was found to significantly elevate HIF-1α protein levels. The AMPK-HIF-1α signaling pathway appears to play a pivotal role in reducing mitochondrial activity and facilitating diapause induction in H. armigera pupae.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1283-1297"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient CRISPR-mediated genome editing can be initiated by embryonic injection but not by ovarian delivery in the beetle Tribolium castaneum. 在甲虫Tribolium castaneum中，胚胎注射可以启动高效的CRISPR介导的基因组编辑，而卵巢注射则不行。

IF 3 1区农林科学

Insect Science Pub Date : 2025-08-01 Epub Date: 2024-09-20 DOI: 10.1111/1744-7917.13447

Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Xing-Ce Wang, Tong-Xian Liu, Hong-Gang Tian

{"title":"Efficient CRISPR-mediated genome editing can be initiated by embryonic injection but not by ovarian delivery in the beetle Tribolium castaneum.","authors":"Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Xing-Ce Wang, Tong-Xian Liu, Hong-Gang Tian","doi":"10.1111/1744-7917.13447","DOIUrl":"10.1111/1744-7917.13447","url":null,"abstract":"The clustered regularly interspaced small palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9)-mediated gene editing technology has revolutionized the study of fundamental biological questions in various insects. Diverse approaches have been developed to deliver the single-guide RNA (sgRNA) and Cas9 to the nucleus of insect embryos or oocytes to achieve gene editing, including the predominant embryonic injection methods and alternative protocols through parental ovary delivery. However, a systematic comparative study of these approaches is limited, especially within a given insect. Here, we focused on revealing the detailed differences in CRISPR/Cas9-mediated gene editing between the embryo and ovary delivery methods in the beetle Tribolium castaneum, using the cardinal and tyrosine hydroxylase (TH) as reporter genes. We demonstrated that both genes could be efficiently edited by delivering Cas9/sgRNA ribonucleoproteins to the embryos by microinjection, leading to the mutant phenotypes and indels in the target gene sites. Next, the Cas9/sgRNA complex, coupled with a nanocarrier called Branched Amphiphilic Peptide Capsules (BAPC), were delivered to the ovaries of parental females to examine the efficacy of BAPC-mediated gene editing. Although we observed that a small number of beetles' progeny targeting the cardinal exhibited the expected white-eye phenotype, unexpectedly, no target DNA indels were found following subsequent sequencing analysis. In addition, we adopted a novel approach termed \"direct parental\" CRISPR (DIPA-CRISPR). However, we still failed to find gene-editing events in the cardinal or TH gene-targeted insects. Our results indicate that the conventional embryonic injection of CRISPR is an effective method to initiate genome editing in T. castaneum. However, it is inefficient by the parental ovary delivery approach.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1185-1200"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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