Insect Science最新文献_第3页

Current paradigms and future challenges in harnessing gut bacterial symbionts of insects for biodegradation of plastic wastes. 利用昆虫肠道细菌共生体对塑料废物进行生物降解的当前模式和未来挑战。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-07-11 DOI: 10.1111/1744-7917.13417

Mudasir A Dar, Rongrong Xie, Hossain M Zabed, Kiran D Pawar, Neeraja P Dhole, Jianzhong Sun

{"title":"Current paradigms and future challenges in harnessing gut bacterial symbionts of insects for biodegradation of plastic wastes.","authors":"Mudasir A Dar, Rongrong Xie, Hossain M Zabed, Kiran D Pawar, Neeraja P Dhole, Jianzhong Sun","doi":"10.1111/1744-7917.13417","DOIUrl":"10.1111/1744-7917.13417","url":null,"abstract":"The ubiquitous incorporation of plastics into daily life, coupled with inefficient recycling practices, has resulted in the accumulation of millions of metric tons of plastic waste, that poses a serious threat to the Earth's sustainability. Plastic pollution, a global problem, disrupts the ecological balance and endangers various life forms. Efforts to combat plastic pollution are underway, with a promising avenue being biological degradation facilitated by certain insects and their symbiotic gut microorganisms, particularly bacteria. This review consolidates existing knowledge on plastic degradation by insects and their influence on gut microbiota. Additionally, it delves into the potential mechanisms employed by insects in symbiosis with gut bacteria, exploring the bioconversion of waste plastics into value-added biodegradable polymers through mineralization. These insights hold significant promise for the bio-upcycling of plastic waste, opening new horizons for future biomanufacturing of high-value chemicals from plastic-derived compounds. Finally, we weigh the pros and cons of future research endeavors related to the bioprospection of plastic-degrading bacteria from underexplored insect species. We also underscore the importance of bioengineering depolymerases with novel characteristics, aiming for their application in the remediation and valorization of waste plastics.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"726-752"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An olfactory model for evaluating the larviposition preference of a vector fly. 用于评估媒介蝇幼虫定位偏好的嗅觉模型。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-07-25 DOI: 10.1111/1744-7917.13426

Jing-Hua Chen, Hui Peng, Shuang Wei, Min-Jun Huang, Rui Tang

引用次数: 0

The odorant-binding protein genes obp67 and obp56d-like encode products that guide oviposition site selection in the Asian tiger mosquito, Aedes albopictus. 气味结合蛋白基因obp67和obp56d-like编码的产物可指导白纹伊蚊选择产卵地点。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-08-12 DOI: 10.1111/1744-7917.13430

Siyu Zhao, Hongkai Liu, Yiming Wu, Peilin Wu, Junyu Fu, Huijuan Yang, Anthony A James, Xiao-Guang Chen

{"title":"The odorant-binding protein genes obp67 and obp56d-like encode products that guide oviposition site selection in the Asian tiger mosquito, Aedes albopictus.","authors":"Siyu Zhao, Hongkai Liu, Yiming Wu, Peilin Wu, Junyu Fu, Huijuan Yang, Anthony A James, Xiao-Guang Chen","doi":"10.1111/1744-7917.13430","DOIUrl":"10.1111/1744-7917.13430","url":null,"abstract":"Aedes albopictus is an important vector of arboviruses and prefers small containers of stagnant water as oviposition sites. One of the mechanisms mosquitoes use to search for suitable oviposition sites is relying on odor cues from prospective sites and their surroundings. The genetic and molecular bases of this behavior are not known for Ae. albopictus. Oviposition site-searching behavior can be separated into 2 stages: container location and water detection. We applied a glue compound to the antennae and the maxillary palps of adult females to mask their ability to detect molecules that may guide them to preferred oviposition sites. Treatment of the antennae significantly reduces the location index (P < 0.001), indicating a decreased ability to find oviposition sites, whereas no significant difference was observed in mosquitoes with maxillary palps treated with the same glue compound (P > 0.05). The detection time, measured as the duration from contact with the water surface to the deposition of the first egg, was extended in mosquitoes with treated antennae or maxillary palps, supporting the conclusion that olfaction is involved in the detection of oviposition site. Transcriptomic analysis identified differentially expressed olfactory-related genes, including obp67, obp56d-like, obp19d-like and obp67-like. RNA interference (RNAi)-mediated knockdown of obp67 and obp56d-like significantly affected the location index and detection time, respectively. Cas9/guide RNA-mediated knockout of obp56d-like resulted in a prolonged detection time, compared with the wild type (P < 0.05). These findings help to elucidate aspects of the olfactory mechanisms involved in Ae. albopictus oviposition site selection, and provide a basis for the development of mosquito surveillance and control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"991-1003"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Larval diet impacts black soldier fly (Diptera: Stratiomyidae) thermal tolerance and preference. 幼虫饮食对黑兵蝇（双翅目：Stratiomyidae）热耐受性和喜好性的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-08-05 DOI: 10.1111/1744-7917.13429

Chujun Li, Travis W Rusch, Amy J Dickerson, Aaron M Tarone, Jeffery K Tomberlin

{"title":"Larval diet impacts black soldier fly (Diptera: Stratiomyidae) thermal tolerance and preference.","authors":"Chujun Li, Travis W Rusch, Amy J Dickerson, Aaron M Tarone, Jeffery K Tomberlin","doi":"10.1111/1744-7917.13429","DOIUrl":"10.1111/1744-7917.13429","url":null,"abstract":"Thermal tolerance and preference are key parameters impacting agricultural production systems. In this study, the impact of larval diet on black soldier fly thermal tolerance and preference across life-stages and sexes was examined. Larvae were fed either a low-protein high-carbohydrate synthetic diet (i.e., P7C35), a high-protein low-carbohydrate synthetic diet (i.e., P35C7), or the Gainesville diet (i.e., C) as a control and reference. Our results demonstrate that the impacts of larval diet on black soldier fly thermal tolerance and preference could be stage and sex specific. The mean heat knockdown temperatures (HKT) ranged between 46.6 and 47.9 °C. Synthetic diets resulted in greater HKT and the difference decreased form larvae (e.g., ∼1 °C) to adults (e.g., ∼0.2 °C). The mean chill-coma recovery time (CCRT) ranged between 8.3 and 21.6 min. Not much differences were detected between diets, but CCRT became longer from larvae to adults. The mean thermal preference ranged between 13.6 and 29.5 °C. Larvae fed synthetic diets preferred much lower temperatures than the control diet. A bimodal distribution was observed for adults regardless of sex. Differences on body mass, lipid, and protein contents were detected among diets; however, more research should be done before any conclusions can be linked to their thermal traits. These findings highlight the importance of considering the ingredients and nutritional makeup of larval diets when optimizing temperature management protocols for mass production of black soldier flies. Conversely, specific diets can be developed to promote survival under extreme rearing temperatures.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1033-1046"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific and interspecific resource partitioning between bumblebee workers and males related to nectar quantity and quality. 熊蜂工蜂和雄蜂之间的种内和种间资源分配与花蜜的数量和质量有关。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-08-05 DOI: 10.1111/1744-7917.13425

Xiao-Fang Jin, Zhong-Ming Ye, Yong-Deng He, Chun-Feng Yang, Michael Orr, Arong Luo, Paul Williams, Chao-Dong Zhu

{"title":"Intraspecific and interspecific resource partitioning between bumblebee workers and males related to nectar quantity and quality.","authors":"Xiao-Fang Jin, Zhong-Ming Ye, Yong-Deng He, Chun-Feng Yang, Michael Orr, Arong Luo, Paul Williams, Chao-Dong Zhu","doi":"10.1111/1744-7917.13425","DOIUrl":"10.1111/1744-7917.13425","url":null,"abstract":"Bumblebees are important pollinators for many natural and agricultural systems in temperate regions. Interspecific and intraspecific variation in floral resource preferences have been proposed to influence bumblebee community structure. In particular, sexual dimorphism is a major source of intraspecific niche variation. Although interspecific resource partitioning is well studied, few studies have explored the intraspecific dynamics between workers and males. Here, we report a study on a total of 11 528 workers and 2220 males of 14 bumblebee species recorded over 5 years in the Hengduan Mountains of Southwest China. We first compared the potential for interspecific and intraspecific competition between workers and males using visitation records and resource partitioning indices (overlap index). We then evaluated the influence of nectar traits on flower preference, including nectar volume and the levels of hexose, sucrose and 10 essential amino acids (EAAs). We found that the niche overlap between intraspecific workers and males was higher than that between different species, and temporal overlap alone did not strongly determine diet overlap. Males of most species preferred flowers with high levels of EAAs and hexose, whereas workers of some species preferred flowers with high nectar volume and sucrose levels. This study suggests that there is floral resource partitioning among bumblebee species, and between workers and males, which may play a key role in alleviating interspecific and intraspecific competition. These findings also provide a useful guide for which kinds of plants might be most valuable for bumblebees, especially the understudied males, in this biodiversity hotspot.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1047-1060"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neighbors sharing pathogens: the intricate relationship between Apis mellifera and ants (Hymenoptera: Formicidae) nesting in hives. 共享病原体的邻居：蜂巢中筑巢的蜜蜂和蚂蚁（膜翅目：蚁科）之间错综复杂的关系。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-08-09 DOI: 10.1111/1744-7917.13433

Rossella Tiritelli, Daniele Giannetti, Enrico Schifani, Donato A Grasso, Giovanni Cilia

{"title":"Neighbors sharing pathogens: the intricate relationship between Apis mellifera and ants (Hymenoptera: Formicidae) nesting in hives.","authors":"Rossella Tiritelli, Daniele Giannetti, Enrico Schifani, Donato A Grasso, Giovanni Cilia","doi":"10.1111/1744-7917.13433","DOIUrl":"10.1111/1744-7917.13433","url":null,"abstract":"Ants are ubiquitous and eusocial insects that exhibit frequent physical contact among colony members, thereby increasing their susceptibility to diseases. Some species are often found in beehives and in their surroundings, where they exploit the food resources of honey bees. This intricate relationship may facilitate the interspecific transmission of honey bee pathogens to ants, although ants themselves may contribute to spillback phenomena. The objective of this study was to assess the presence and abundance of honey bee pathogens in ants sampled from Italian apiaries. A total of 37 colonies within 24 apiaries across 7 regions were monitored. In total, 6 pathogens were detected in adult ants and 3 in the brood. In particular, the study revealed a high prevalence of honey bee pathogens in ants, with DWV, BQCV, and CBPV being the most commonly encountered. The brood also tested positive for the same viruses. Notably, all analyzed viruses were found to be replicative in both adult ants and ant broods. Furthermore, co-infections were prevalent, suggesting complex pathogen interactions within ant populations. Statistical analysis indicated significant differences in pathogen prevalence and abundance among ant species and sample types. The findings highlight active infection in both the ants and the brood, suggesting a potential role of ants as reservoir hosts and vectors of honey bee pathogens emphasizing the need for further research to understand the implications of interspecific pathogen transmission on ant and bee health.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"943-956"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application. 表达生物活性人类睫状神经营养因子的转基因家蚕在生物医学中的应用。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-09-01 DOI: 10.1111/1744-7917.13442

Weiqun Lan, Wenjing Geng, Xuechun Jiang, Feng Chen, Mingyi Zhou, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li

{"title":"Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application.","authors":"Weiqun Lan, Wenjing Geng, Xuechun Jiang, Feng Chen, Mingyi Zhou, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li","doi":"10.1111/1744-7917.13442","DOIUrl":"10.1111/1744-7917.13442","url":null,"abstract":"Ciliary neurotrophic factor (CNTF) acts as a potent neuroprotective agent in neuronal survival and regeneration, and can also induce the differentiation of several stem cells into neurons, which highlights the broad application of CNTF in biomedicine. However, large-scale production of bioactive recombinant human CNTF protein remains to be explored. Herein, this study aims to express a bioactive human CNTF protein on a large scale by genetically engineering a silk gland bioreactor of silkworm. Our results showed that CNTF protein was successfully expressed in the middle silk gland (MSG) of silkworm, which can be secreted into the silks with the amount of 3.2 mg/g cocoons. The fabrication of human CNTF-functionalized silk material was able to promote proliferation and migration of neural cells when compared to the natural silk protein. Importantly, this functional silk material could also facilitate neurite outgrowth of mouse retinal ganglion cell (RGC-5) cells. All these data demonstrated a high bioactivity of the recombinant human CNTF protein expressed in the MSG of silkworm. The further fabrication of different silk materials with CNTF bioactivity will give biomedical applications in tissue engineering and neuroregeneration.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"809-820"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142106999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A long cold storage protocol for Anisopteromalus calandrae based on promoting prepupal development and arresting early pupal growth under low temperature. 基于在低温条件下促进蛹前期发育和抑制蛹早期生长的卡兰德拉蝶（Anisopteromalus calandrae）长期冷藏方案。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-08-12 DOI: 10.1111/1744-7917.13436

Jiapeng Yang, Yue Zhang, Li Liu, Die Liu, Guy Smagghe, Maofa Yang, Xiaoyulong Chen, Hong Yang, Renhuai Dai

{"title":"A long cold storage protocol for Anisopteromalus calandrae based on promoting prepupal development and arresting early pupal growth under low temperature.","authors":"Jiapeng Yang, Yue Zhang, Li Liu, Die Liu, Guy Smagghe, Maofa Yang, Xiaoyulong Chen, Hong Yang, Renhuai Dai","doi":"10.1111/1744-7917.13436","DOIUrl":"10.1111/1744-7917.13436","url":null,"abstract":"Anisopteromalus calandrae (Howard) shows great promise as an ectoparasitoid for controlling various coleopteran pests in warehouses. However, for a large-scale release, it is crucial to establish an ample supply of A. calandrae while carefully maintaining their quality and effectiveness. Appropriate cold storage techniques are the key to achieving these goals. Previous studies on cold storage have focused on specific developmental stages and explored cold storage conditions that can be applied only to those stages. Herein, we examined the development, survival and reproductive capacity of A. calandrae at different temperatures (13, 16, and 19 °C) and storage durations (30, 60, and 90 d) and evaluated the fitness of the offspring. A. calandrae completed its egg-to-larva development and pupated at 16 °C, but its development was arrested at an early pupal stage. Even after 90 d of cold storage at 16 °C, the survival rate of A. calandrae remained high at 77%, with no significant impact on reproductive capacity. Furthermore, cold storage showed no negative effect on the F1 generation. In contrast, eggs stored at 13 °C failed to hatch, whereas those stored at 19 °C developed. Adults emerged after > 60 d. This indicates that storage at 19 °C is only suitable for short durations. Our findings highlight the developmental pattern of A. calandrae at 16 °C, indicating that the parasitic wasp can be stored for a long time at this temperature across all stages of development before pupation, substantially facilitating its mass reproduction and industrial production.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1019-1032"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The beetle's structural protein CPCFC making elytra tough and rigid. 甲虫的结构蛋白 CPCFC 使蜕皮坚硬无比。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-09-05 DOI: 10.1111/1744-7917.13443

Han Bao, Yuantao Liu, Yanwei Duan, Lei Chen, Qing Yang

{"title":"The beetle's structural protein CPCFC making elytra tough and rigid.","authors":"Han Bao, Yuantao Liu, Yanwei Duan, Lei Chen, Qing Yang","doi":"10.1111/1744-7917.13443","DOIUrl":"10.1111/1744-7917.13443","url":null,"abstract":"The insect cuticle, which serves as both a protective barrier and an efficient lever system for locomotion, is an extracellular matrix primarily composed of chitin and protein. The cuticle protein CPCFC characterized by a \"CFC\" motif containing 2 Cys split by the insertion of 5 residues is distributed across most insect species and specifically localized in the hard part of the cuticle. However, their physiological function is not fully understood. Here, we report 2 CPCFC proteins, TcCPCFC1 and TcCPCFC2, derived from the Coleopteran insect Tribolium castaneum. We revealed that TcCPCFC1 and TcCPCFC2 were predominantly expressed during the larval and adult stages of T. castaneum, respectively. The transcription downregulation of TcCPCFC1 significantly decreased the modulus and toughness of the elytral cuticle. We found that TcCPCFC proteins have high binding affinity to chitin. We cloned and produced recombinant TcCPCFC proteins and demonstrated that the addition of TcCPCFC proteins to chitin hydrogel greatly enhanced the hydrogel's modulus and toughness by forming denser chitin fibrous networks. Our findings reveal the functional role of CPCFC proteins in enhancing mechanical properties of insect cuticle, and we validate this process in vitro, and offer a protein candidate for fabrication of advanced chitin-based materials.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"821-832"},"PeriodicalIF":2.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-kingdom regulation of silkworm development and cocooning by mulberry microRNAs. 桑树微RNA对家蚕发育和结茧的跨域调控。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-06-01 Epub Date: 2024-07-17 DOI: 10.1111/1744-7917.13424

Xinyan Zhou, Jingwei Guo, Chao Qin, Ping Qian, Jiubo Liang, Jinyu Fu, Xinjia Shen, Ningjia He, Chen-Yu Zhang, Xi Chen

引用次数: 0