Insect Science最新文献_第3页

RNAi-mediated pest control targeting the Troponin I (wupA) gene in sweet potato weevil, Cylas formicarius. 针对甘薯象鼻虫 Troponin I (wupA) 基因的 RNAi- 介导害虫控制。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-11 DOI: 10.1111/1744-7917.13403

Mengjun Zhang, Xiaxuan Zhang, Tingting Chen, Yonglin Liao, Bin Yang, Guirong Wang

{"title":"RNAi-mediated pest control targeting the Troponin I (wupA) gene in sweet potato weevil, Cylas formicarius.","authors":"Mengjun Zhang, Xiaxuan Zhang, Tingting Chen, Yonglin Liao, Bin Yang, Guirong Wang","doi":"10.1111/1744-7917.13403","DOIUrl":"10.1111/1744-7917.13403","url":null,"abstract":"The sweet potato weevil (Cylas formicarius) is a critical pest producing enormous global losses in sweet potato crops. Traditional pest management approaches for sweet potato weevil, primarily using chemical pesticides, causes pollution, food safety issues, and harming natural enemies. While RNA interference (RNAi) is a promising environmentally friendly approach to pest control, its efficacy in controlling the sweet potato weevil has not been extensively studied. In this study, we selected a potential target for controlling C. formicarius, the Troponin I gene (wupA), which is essential for musculature composition and crucial for fundamental life activities. We determined that wupA is abundantly expressed throughout all developmental stages of the sweet potato weevil. We evaluated the efficiency of double-stranded RNAs in silencing the wupA gene via microinjection and oral feeding of sweet potato weevil larvae at different ages. Our findings demonstrate that both approaches significantly reduced the expression of wupA and produced high mortality. Moreover, the 1st instar larvae administered dswupA exhibited significant growth inhibition. We assessed the toxicity of dswupA on the no-target insect silkworm and assessed its safety. Our study indicates that wupA knockdown can inhibit the growth and development of C. formicarius and offer a potential target gene for environmentally friendly control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"631-648"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of two transglutaminases on innate immune responses in the oriental armyworm, Mythimna separata. 两种转谷氨酰胺酶对东方草履虫先天免疫反应的影响

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-10 DOI: 10.1111/1744-7917.13420

Ying Zhu, Seiichi Furukawa

{"title":"Effects of two transglutaminases on innate immune responses in the oriental armyworm, Mythimna separata.","authors":"Ying Zhu, Seiichi Furukawa","doi":"10.1111/1744-7917.13420","DOIUrl":"10.1111/1744-7917.13420","url":null,"abstract":"Transglutaminase (TGase) is a key enzyme that mediates hemolymph coagulation and is thought to contribute to the elimination of pathogenic microorganisms in invertebrates. The objective of this study was to elucidate the involvement of TGase in insect immune responses via functional analysis of this enzyme in the oriental armyworm, Mythimna separata, using recombinant proteins and RNA interference technique. We identified two TGase genes, mystgase1 and mystgase2, in Mythimna separata and found that both genes are expressed in all surveyed tissues in M. separata larvae. Significant changes were induced in hemocytes following Escherichia coli injection. Injection of Gram-positive bacteria (Micrococcus luteus) and Gram-negative bacteria (Escherichia coli and Serratia marcescens) into larvae triggered a time-specific induction of both mystgase1 and mystgase2 in hemocytes. Recombinant MysTGase1 and MysTGase2 proteins bound to both E. coli and M. luteus, localizing within bacterial clusters and resulting in agglutination in a Ca2+-dependent manner. The hemocytes of larvae injected with recombinant MysTGase1 or MysTGase2 exhibited enhanced phagocytic ability against E. coli, improved in vivo bacterial clearance, and increased resistance to S. marcescens, decreasing larval mortality rate. Conversely, RNA interference targeting mystgase1 or mystgase2 significantly reduced hemocyte phagocytic capability, decreased bacterial clearance, and increased susceptibility to S. marcescens infection, thereby increasing larval mortality rate. The findings of this study are anticipated to expand our understanding of the function of TGases within insect immune responses and may contribute to developing new pest control strategies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"409-424"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo functional analysis of the cotton bollworm Helicoverpa armigera 24-dehydrocholesterol reductase (HaDHCR24) in phytosterol metabolism. 棉铃虫 24-脱氢胆固醇还原酶（HaDHCR24）在植物甾醇代谢中的体内功能分析。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-08 DOI: 10.1111/1744-7917.13405

Zhen Yang, Jinchan Ren, Shuning Lu, Yuanze Feng, Yongliang Fan, Tong-Xian Liu, Xiangfeng Jing

{"title":"In vivo functional analysis of the cotton bollworm Helicoverpa armigera 24-dehydrocholesterol reductase (HaDHCR24) in phytosterol metabolism.","authors":"Zhen Yang, Jinchan Ren, Shuning Lu, Yuanze Feng, Yongliang Fan, Tong-Xian Liu, Xiangfeng Jing","doi":"10.1111/1744-7917.13405","DOIUrl":"10.1111/1744-7917.13405","url":null,"abstract":"Insects have to obtain sterols from food due to the inability to synthesize this essential nutrient de novo. For lepidopteran insects, they can convert a variety of phytosterols into cholesterol to meet their growth needs. The final step of the cholesterol biosynthesis is the metabolism of desmosterol catalyzed by 24-dehydrocholesterol reductase (DHCR24). In this study, we identified a DHCR24 homolog in the cotton bollworm Helicoverpa armigera, designated as H. armigera 24-dehydrocholesterol reductase (HaDHCR24)-1. The quantitative expression analyses indicated that HaDHCR24-1 was highly enriched in the midgut where dietary sterol uptake occurs. Compared to the control, the DHCR24-1 mutant larvae generated by clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 technology accumulated more desmosterol in the gut, while the content of cholesterol was significantly reduced. A similar phenomenon was observed when the DHCR24 inhibitor, amiodarone, was applied to the insects. Moreover, DHCR24-1 played an important role for the usage of β-sitosterol, a major sterol in plants, in H. armigera, and loss of function of DHCR24-1 resulted in higher mortality on β-sitosterol. However, the DHCR24 homolog does not necessarily exist in the genomes of all insects. The loss of this gene occurred more frequently in the insects feeding on animals, which further support the role of DHCR24-1 in using phytosterols. This gene may have important potential in developing new strategies to control herbivory pests in Lepidoptera and other insect orders.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"398-408"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of CO₂ elevation on life-history traits of two insecticide-resistant strains of planthopper Nilaparvata lugens on rice. 二氧化碳浓度升高对水稻上两种抗虫性扑粉虱 Nilaparvata lugens 生活史特征的影响。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-21 DOI: 10.1111/1744-7917.13416

Hongran Li, Shanshan Wu, Jing Liu, Yong Chen, Ling Meng, Baoping Li

{"title":"Effects of CO2 elevation on life-history traits of two insecticide-resistant strains of planthopper Nilaparvata lugens on rice.","authors":"Hongran Li, Shanshan Wu, Jing Liu, Yong Chen, Ling Meng, Baoping Li","doi":"10.1111/1744-7917.13416","DOIUrl":"10.1111/1744-7917.13416","url":null,"abstract":"We made separate experiments to examine life-history traits and activities of protective enzymes as affected by carbon dioxide (CO2) elevation to 780 μL/L as compared to 390 μL/L in imidacloprid- or buprofezin-resistant strains of the brown planthopper (BPH) Nilaparvata lugens. We found an interaction effect between resistance and the CO2 level on the nymphal survival and duration in both resistant strains. Nymphal durations in both resistant strains were much shorter in the resistant than susceptible BPH at 780 μL/L but similar between them or slightly shorter in the resistant than susceptible BPH at 390 μL/L. Nymphal survival was lower for imidacloprid-resistant than its susceptible BPH at 390 μL/L but higher at 780 μL/L; it stayed unaffected by the CO2 elevation in buprofezin-resistant BPH. We did not observe an interaction effect between resistance and the CO2 level on major reproductive parameters in both resistant strains. But the 2 strains were not consistent across CO2 levels in all parameters. Our measurements of protective enzyme activities of superoxide dismutase, catalase, and peroxidase showed an interaction between resistance and the CO2 level. Overall, these enzymes became similar in activity between resistant and susceptible BPH at 780 μL/L compared to 390 μL/L and the change was more distinct in the imidacloprid- than buprofezin-resistant BPH strains. Our findings suggest that CO2 elevation can affect life-history traits of insecticide-resistant BPH, while the effect may vary depending on the kind of insecticides it is resistant to.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"701-711"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel treatment approach using vitamin B12-conjugated sericin for mitigating nanodiamond-induced toxicity in darkling beetles. 利用维生素 B12 结合的丝胶蛋白减轻纳米金刚石诱发的暗纹甲虫毒性的新型治疗方法。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-07-16 DOI: 10.1111/1744-7917.13421

Marwa Saad, Nabila Selim, Lamia M El-Samad

{"title":"A novel treatment approach using vitamin B12-conjugated sericin for mitigating nanodiamond-induced toxicity in darkling beetles.","authors":"Marwa Saad, Nabila Selim, Lamia M El-Samad","doi":"10.1111/1744-7917.13421","DOIUrl":"10.1111/1744-7917.13421","url":null,"abstract":"The escalating use of nanodiamonds (NDs) has raised concerns about their ecotoxicological impact, prompting exploration of therapeutic interventions. This paper pioneers the examination of Vitamin B12-conjugated sericin (VB12-SER) as a potential therapeutic approach against ND-induced toxicity in darkling beetles (Blaps polychresta). The study analyzes mortality rates and organ-specific effects, covering the testis, ovary, and midgut, before and after treatments. Following exposure to 10 mg NDs/g body weight, within a subgroup of individuals termed ND2 with a mortality rate below 50%, two therapeutic treatments were administered, including pure sericin (SER) at 10 mg/mL and VB12-SER at 10.12 mg/mL. Consequently, five experimental groups (control, SER, ND2, ND2+SER, ND2+SER+VB12) were considered. Kaplan-Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period. Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs, including cell death, DNA damage, enzyme dysregulation, antioxidant imbalances, protein depletion, lipid peroxidation, and morphological deformities. In contrast, the proposed treatments, especially (ND2+SER+VB12), demonstrated remarkable recovery, highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects. Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins. This research enhances understanding of NDs' effects on B. polychresta, proposing it as an effective bioindicator, and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"551-584"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignin disruption and ligninolytic enzyme activity in the symbiotic system of the Macrotermes barneyi termite.

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 DOI: 10.1111/1744-7917.70026

Farhan Ahmad, Hu Jinhao, Muhammad Zohaib Nawaz, Mudasir A Dar, Raghda Nasser, Syed Zeeshan Haider, Waqar Ul Haq, Jianzhong Sun, Jianchu Mo, Daochen Zhu

{"title":"Lignin disruption and ligninolytic enzyme activity in the symbiotic system of the Macrotermes barneyi termite.","authors":"Farhan Ahmad, Hu Jinhao, Muhammad Zohaib Nawaz, Mudasir A Dar, Raghda Nasser, Syed Zeeshan Haider, Waqar Ul Haq, Jianzhong Sun, Jianchu Mo, Daochen Zhu","doi":"10.1111/1744-7917.70026","DOIUrl":"https://doi.org/10.1111/1744-7917.70026","url":null,"abstract":"Fungus-farming termites efficiently degrade recalcitrant lignocellulose through a symbiotic relationship with Termitomyces and the gut microbiome, making them successful key decomposers in (sub)tropical ecosystems. Despite extensive research on plant biomass decomposition, the mechanisms of lignin degradation in fungus-farming termites remain elusive. In view of this information gap, the present study employed several analytical approaches and ligninolytic enzyme assays to investigate lignin modification in the symbiotic system of a fungus-farming termite, Macrotermes barneyi. The results revealed the structural modification of lignin across different points of the degradation process. Enzyme assays of termite guts and fungus combs showed the obvious differences in ligninolytic enzyme activity at different sites of decomposition, likely initiating the modification of lignin. The findings of the current study support the hypothesis that although young workers start the modification of lignin to some extent, they largely leave the lignin monomers p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S) intact. Most of the lignin-derived compounds are transferred to the fresh comb, where the majority of lignin modification begins and continues in mature and older parts of the comb. This study provides new insights into biomass degradation within the microsymbiotic system of an insect. A better understanding of these mechanisms has the promising potential for unlocking new lignin-degrading agents for the production of renewable energy.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

microRNA miR-315-5p regulates developmental growth in Drosophila wings by targeting S6k.

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 DOI: 10.1111/1744-7917.70027

Taeheon Lee, Chae Jeong Kim, Do-Hwan Lim, Young Sik Lee

{"title":"microRNA miR-315-5p regulates developmental growth in Drosophila wings by targeting S6k.","authors":"Taeheon Lee, Chae Jeong Kim, Do-Hwan Lim, Young Sik Lee","doi":"10.1111/1744-7917.70027","DOIUrl":"https://doi.org/10.1111/1744-7917.70027","url":null,"abstract":"Tissue growth in Drosophila is regulated by various factors, with microRNAs (miRNAs) emerging as key players over the past decade. However, the precise roles of miRNAs in growth regulation remain incompletely understood. In this study, we explored the biological role of miR-315 in wing growth regulation. Inhibition of miR-315-5p activity using a miR-315 sponge led to an increase in wing size, whereas its overexpression resulted in reduced wing size, primarily through a decrease in wing cell size. We identified ribosomal protein kinase p-70-S6k (S6k) as a target of miR-315-5p in relation to wing growth control. Overexpression of miR-315 reduced both total S6k and phosphorylated S6k protein levels in Drosophila S2 cells and wing discs. Additionally, a luciferase reporter assay confirmed that miR-315-5p directly binds to the 3'-untranslated region of S6k. Consistently, RNAi-mediated depletion of S6k led to smaller wings, primarily due to a reduction in cell size. Notably, co-overexpression of active S6k rescued the wing defects caused by miR-315 overexpression. Overall, these findings demonstrate that miR-315 regulates wing growth by suppressing S6k expression.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of vitellogenin receptor based on minute insect RNA interference methods affects the initial mature egg load in the pest natural enemy Trichogramma dendrolimi. 基于微小昆虫 RNA 干扰方法的卵黄素受体敲除会影响害虫天敌 Trichogramma dendrolimi 的初始成熟卵量。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-05-23 DOI: 10.1111/1744-7917.13385

Cheng-Xing Wang, Hui-Qiao Bao, Zhi-Chao Yan, Jie Wang, Su Wang, Yuan-Xi Li

{"title":"Knockdown of vitellogenin receptor based on minute insect RNA interference methods affects the initial mature egg load in the pest natural enemy Trichogramma dendrolimi.","authors":"Cheng-Xing Wang, Hui-Qiao Bao, Zhi-Chao Yan, Jie Wang, Su Wang, Yuan-Xi Li","doi":"10.1111/1744-7917.13385","DOIUrl":"10.1111/1744-7917.13385","url":null,"abstract":"Vitellogenin receptor (VgR) plays a crucial role in oogenesis by mediating endocytosis of vitellogenin and a portion of the yolk proteins in many insect species. However, the function of VgR in minute parasitoid wasps is largely unknown. Here, we applied Trichogramma dendrolimi, a minute egg parasitoid, as a study model to investigate the function of VgR in parasitoids. We developed RNA interference (RNAi) methods based on microinjection of prepupae in T. dendrolimi. RNAi employs nanomaterial branched amphipathic peptide capsules (BAPC) as a carrier for double-stranded RNA (dsRNA), significantly enhancing delivery efficiency. Also, artificial hosts without medium were used to culture the injected prepupae in vitro. Utilizing these methods, we found that ovarian growth was disrupted after knockdown of TdVgR, as manifested by the suppressed development of the ovariole and the inhibition of nurse cell internalization by oocytes. Also, the initial mature egg load in the ovary was significantly reduced. Notably, the parasitic capacity of the female adult with ovarian dysplasia was significantly decreased, possibly resulting from the low availability of mature eggs. Moreover, ovarian dysplasia in T. dendrolimi caused by VgR deficiency are conserved despite feeding on different hosts. The results confirmed a critical role of TdVgR in the reproductive ability of T. dendrolimi and provided a reference for gene functional studies in minute insects.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"487-500"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome study reveals tick immune genes restrict Babesia microti infection. 转录组研究揭示蜱免疫基因限制小尾丝虫感染。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-04 DOI: 10.1111/1744-7917.13384

Tingting Feng, Hao Tong, Feihu Zhang, Qianqian Zhang, Heng Zhang, Xia Zhou, Hang Ruan, Qihan Wu, Jianfeng Dai

{"title":"Transcriptome study reveals tick immune genes restrict Babesia microti infection.","authors":"Tingting Feng, Hao Tong, Feihu Zhang, Qianqian Zhang, Heng Zhang, Xia Zhou, Hang Ruan, Qihan Wu, Jianfeng Dai","doi":"10.1111/1744-7917.13384","DOIUrl":"10.1111/1744-7917.13384","url":null,"abstract":"A systems biology approach was employed to gain insight into tick biology and interactions between vectors and pathogens. Haemaphysalis longicornis serves as one of the primary vectors of Babesia microti, significantly impacting human and animal health. Obtaining more information about their relationship is crucial for a comprehensive understanding of tick and pathogen biology, pathogen transmission dynamics, and potential control strategies. RNA sequencing of uninfected and B. microti-infected ticks resulted in the identification of 15 056 unigenes. Among these, 1 051 were found to be differentially expressed, with 796 being upregulated and 255 downregulated (P < 0.05). Integrated transcriptomics datasets revealed the pivotal role of immune-related pathways, including the Toll, Janus kinase/signal transducer and activator of transcription (JAK-STAT), immunodeficiency, and RNA interference (RNAi) pathways, in response to infection. Consequently, 3 genes encoding critical transcriptional factor Dorsal, Relish, and STAT were selected for RNAi experiments. The knockdown of Dorsal, Relish, and STAT resulted in a substantial increase in Babesia infection levels compared to the respective controls. These findings significantly advanced our understanding of tick-Babesia molecular interactions and proposed novel tick antigens as potential vaccine targets against tick infestations and pathogen transmission.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"457-470"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candidatus Liberibacter asiaticus influences the emergence of the Asian citrus psyllid Diaphorina citri by regulating key cuticular proteins. 亚洲自由杆菌通过调节关键的角质蛋白影响亚洲柑橘木虱Diaphorina citri的萌发。

IF 2.9 1区农林科学

Insect Science Pub Date : 2025-04-01 Epub Date: 2024-06-16 DOI: 10.1111/1744-7917.13404

Chen-Yang Yuan, Yi-Fan Gao, Yi Liu, Jia-Yao Fan, Ying-Zhe Yuan, Long Yi, Tian-Xing Jing, Wei Dou, Jin-Jun Wang

{"title":"Candidatus Liberibacter asiaticus influences the emergence of the Asian citrus psyllid Diaphorina citri by regulating key cuticular proteins.","authors":"Chen-Yang Yuan, Yi-Fan Gao, Yi Liu, Jia-Yao Fan, Ying-Zhe Yuan, Long Yi, Tian-Xing Jing, Wei Dou, Jin-Jun Wang","doi":"10.1111/1744-7917.13404","DOIUrl":"10.1111/1744-7917.13404","url":null,"abstract":"The Asian citrus psyllid, Diaphorina citri, is the primary vector of the HLB pathogen, Candidatus Liberibacter asiaticus (CLas). The acquisition of CLas shortens the developmental period of nymphs, accelerating the emergence into adulthood and thereby facilitating the spread of CLas. Cuticular proteins (CPs) are involved in insect emergence. In this study, we investigated the molecular mechanisms underlying CLas-promoted emergence in D. citri via CP mediation. Here, a total of 159 CP genes were first identified in the D. citri genome. Chromosomal location analysis revealed an uneven distribution of these CP genes across the 13 D. citri chromosomes. Proteomic analysis identified 54 differentially expressed CPs during D. citri emergence, with 14 CPs exhibiting significant differential expression after CLas acquisition. Five key genes, Dc18aa-1, Dc18aa-2, DcCPR-24, DcCPR-38 and DcCPR-58, were screened from the proteome and CLas acquisition. The silencing of these 5 genes through a modified feeding method significantly reduced the emergence rate and caused various abnormal phenotypes, indicating the crucial role that these genes play in D. citri emergence. This study provides a comprehensive overview of the role of CPs in D. citri and reveals that CLas can influence the emergence process of D. citri by regulating the expression of CPs. These key CPs may serve as potential targets for future research on controlling huanglongbing (HLB) transmission.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"501-514"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0