Insect Science最新文献_第4页

Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae). 用捕食性螨（大螯螨螨科）、大螯螨（大螯螨科）和大螯螨（大螯螨科）防治蘑菇栽培中Lycoriella小蚊。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-08-11 DOI: 10.1111/1744-7917.70141

Qing-Xiu Lan, Mei-Fang Wen, Zheng-Hui Lu, Bing-Rong Ke, Qing-Hai Fan, Min-Sheng You

{"title":"Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae).","authors":"Qing-Xiu Lan, Mei-Fang Wen, Zheng-Hui Lu, Bing-Rong Ke, Qing-Hai Fan, Min-Sheng You","doi":"10.1111/1744-7917.70141","DOIUrl":"10.1111/1744-7917.70141","url":null,"abstract":"Fungus gnats, Lycoriella spp. (Diptera: Sciaridae), are significant pests in edible mushroom cultivation worldwide. This study evaluates the effectiveness of 2 soil predatory mites, Stratiolaelaps scimitus (Laelapidae) and Macrocheles glaber (Macrochelidae), in controlling fungus gnats density on the mushroom Agrocybe aegerita. A controlled experiment was performed to examine and compare the effects of individual and combined releases of the 2 mite species on fungus gnats populations and their impact on mushroom yield. Results showed that both mite species significantly reduced fungus gnat populations as compared to untreated controls. However, S. scimitus exhibited superior control, effectively suppressing adult fungus gnats compared to the controls, single release of M. glaber and combined releases (M. glaber + S. scimitus). Mushroom yield was highest in the S. scimitus treatment, with no significant differences observed among other treatments and controls. Interestingly, neither mite species negatively impacted mushroom quality or budding. These findings highlight the significance of S. scimitus as a highly effective biological control agent for suppression of fungus gnats, thereby contributing to sustainable pest management practices in mushroom cultivation.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2149-2156"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor Nlci is essential for wing dimorphism in the brown planthopper, Nilaparvata lugens. 转录因子Nlci在褐飞虱翅膀二态性中起重要作用。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-27 DOI: 10.1111/1744-7917.70127

Dong Wen, He Jiang, Zhuangzhuang Qiao, Zewei Lu, Muhua Zhao, Miaomiao Tian, Shaoqin Li, Hongxia Hua

{"title":"Transcription factor Nlci is essential for wing dimorphism in the brown planthopper, Nilaparvata lugens.","authors":"Dong Wen, He Jiang, Zhuangzhuang Qiao, Zewei Lu, Muhua Zhao, Miaomiao Tian, Shaoqin Li, Hongxia Hua","doi":"10.1111/1744-7917.70127","DOIUrl":"10.1111/1744-7917.70127","url":null,"abstract":"The brown planthopper (BPH) alternatively develops into either macropterous or brachypterous morphs in response to environmental stimuli, enhancing its fitness and dispersal. Despite several key upstream regulator genes involved in wing morphs switching having been identified, the downstream pathways governing wing plasticity remain unclear. Here, we harvested the 4th instar nymphal wing buds from macropterous strain (MS) and brachypterous strain (BS) for transcriptome sequencing. Individually, 3 025 and 3 273 differentially expressed genes (DEGs) were identified in forewing and hindwing buds, respectively, between 2 strains. Functional enrichment analysis revealed that these DEGs were significantly enriched in various categories related to signal transduction and metabolism. Among these, the Hedgehog (Hh) pathway was implicated in environmental information processing and wing development regulating. Knocking down the transcription factor Cubitus interruptus (Nlci) of the Hh pathway significantly reduced the wing size and resulted in malformed wings of both strain adults. Additionally, the expression levels of downstream genes (Nlptc, Nlen, and Nldpp) were inhibited to varying degrees. Furthermore, the expression levels of Nlci and its downstream genes were decreased when injected with double-stranded (ds)NlInR1, but increased in dsNlInR2 treatment. Silencing of Nlci partially antagonized the acceleration effect of dsNlInR2 treatment on wing development, leading to an intermediate wing morph. Our findings expand the regulatory role of the Hh pathway in the wing dimorphism of BPH, providing new insights into the mechanisms underlying wing development.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1782-1796"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144730194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a dual-loop double-stranded RNA to control small brown planthopper. 双环双链RNA在小褐飞虱控制中的应用。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-14 DOI: 10.1111/1744-7917.70128

Panpan Ge, Xiangyang Lu, Wan Zhao, Shaohui Wu, Neil Smith, Ling-Ling Gao, Hui-Shan Guo, Ming-Bo Wang, Feng Cui

{"title":"Application of a dual-loop double-stranded RNA to control small brown planthopper.","authors":"Panpan Ge, Xiangyang Lu, Wan Zhao, Shaohui Wu, Neil Smith, Ling-Ling Gao, Hui-Shan Guo, Ming-Bo Wang, Feng Cui","doi":"10.1111/1744-7917.70128","DOIUrl":"10.1111/1744-7917.70128","url":null,"abstract":"RNA interference, induced by double-stranded RNA (dsRNA), has emerged as a biopesticide for pest control. However, the stability of dsRNA remains a challenge in topical delivery, preventing widespread agricultural application. Here, we tested a dsRNA variant, called loop-ended dsRNA or LedRNA, for controlling a notorious vector insect, the small brown planthopper (SBPH). LedRNA is characterized by having a loop at both ends of the dsRNA stem, resembling a close-circled RNA. Three SBPH target genes, including Flotillin2, which is vital for viral transmission, and trehalose-6-phosphate synthase (TPS) and malate dehydrogenases (MDH), which are essential for insect survival, were tested for gene silencing efficiencies through microinjection, soaking, or oral delivery of the conventional dsRNA, hairpin dsRNA, and LedRNA. All 3 molecules induced effective gene silencing using microinjection, but did not suppress target gene expression through soaking. Notably, LedRNA demonstrated superior efficacy in inducing gene silencing in SBPH through oral feeding compared to the other 2 molecules. Silencing efficiencies ranged from 26% to 39% for Flotillin2, 39% to 51% for TPS, and 45% to 64% for MDH. When SBPH were fed Escherichia coli-synthesized LedRNA targeting these 3 genes, silencing efficiencies ultimately reached 28% to 74%, leading to reduced insect survival and decreased viral transmission in host plant rice. This study highlights the potential of LedRNA as a biopesticide for insect pest control.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1769-1781"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transovarial transmission of Wolbachia bacteria via P44/Msp2-IMP2 mediated endocytosis. 通过P44/Msp2-IMP2介导的胞吞作用传播沃尔巴克氏菌。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-18 DOI: 10.1111/1744-7917.70129

Lu Ren, Yan-Jun Men, Xiao-Li Bing, Ary Hoffmann, Xiao-Yue Hong

{"title":"Transovarial transmission of Wolbachia bacteria via P44/Msp2-IMP2 mediated endocytosis.","authors":"Lu Ren, Yan-Jun Men, Xiao-Li Bing, Ary Hoffmann, Xiao-Yue Hong","doi":"10.1111/1744-7917.70129","DOIUrl":"10.1111/1744-7917.70129","url":null,"abstract":"Wolbachia is a maternally inherited endosymbiont that stably localizes in oocytes of arthropods to ensure successful transovarial transmission. However, the source of Wolbachia in oocytes is unclear. Here, we obtained a Wolbachia strain that is transovarially transmitted with complete fidelity in the agricultural pest spider mite Tetranychus truncatus. Using fluorescence in situ hybridization, we showed that Wolbachia migrate from the digestive cells to the reproductive system as a female develops. When Wolbachia from T. truncatus was transferred to Aedes albopictus cells, we demonstrated that its cell-to-cell spread was regulated by the host's endocytosis system. By assaying the proteome, we identified a bacterial surface protein P44/Msp2 from Wolbachia that interacted with the host's integral membrane protein 2 (IMP2). RNA interference of IMP2 indicated that it facilitated entry of Wolbachia. Our results show that Wolbachia from somatic cells contribute to its transovarial transmission, and that Wolbachia also utilize the surface protein to invade new host cells. This extends our understanding of how Wolbachia is transmitted between cells and facilitates artificial transfection work.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2040-2052"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two transcription factor cascades regulate odorant-binding protein NlOBP8 affecting the recognition of linalool in the brown planthopper Nilaparvata lugens. 两个转录因子级联调节气味结合蛋白NlOBP8，影响褐飞虱对芳樟醇的识别。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-14 DOI: 10.1111/1744-7917.70118

Ruilin Zhang, Shuanggang Duan, Sara Taha Abdelkhalek, Ao Liu, Chao Wang, Yi Wei, Ruinan Yang, Manqun Wang

{"title":"Two transcription factor cascades regulate odorant-binding protein NlOBP8 affecting the recognition of linalool in the brown planthopper Nilaparvata lugens.","authors":"Ruilin Zhang, Shuanggang Duan, Sara Taha Abdelkhalek, Ao Liu, Chao Wang, Yi Wei, Ruinan Yang, Manqun Wang","doi":"10.1111/1744-7917.70118","DOIUrl":"10.1111/1744-7917.70118","url":null,"abstract":"The insect olfactory system is pivotal in mediating chemical-driven behaviors, including host plant selection, mate identification, oviposition site localization, and ecological niche recognition. Although odorant-binding proteins (OBPs) are known to facilitate olfactory signal transduction in insects, the transcriptional regulatory mechanisms governing their expression remain elusive. Here, we identified 2 transcriptional cascades involving the transcription factors (TFs) distal-less (Dll) and aristaless (al) that converge to regulate NlObp8 expression in the brown planthopper (Nilaparvata lugens, BPH) through bioinformatics and transcriptomic analysis. The functional impact of the 2 TFs was validated using the dual-luciferase reporter assays and yeast 1-hybrid assays. RNA interference-mediated knockdown of Nlal significantly downregulated NlObp8 expression and abolished the repellent behavioral response of BPHs to linalool. Furthermore, silencing of NlDll resulted in significant downregulation of Nlal expression. These results revealed that the cascade regulatory mechanism, involving direct al binding and indirect Dll-mediated transcriptional activation, provided molecular targets for disrupting olfactory-driven behaviors in agricultural pest management.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1903-1914"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The forewing-hindwing coupling of a black cicada Cryptotympana atrata (Hemiptera: Cicadidae): Functional morphology and kinematics. 黑蝉（半翅目：蝉科）前翅-后翅耦合：功能形态学和运动学。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-08-06 DOI: 10.1111/1744-7917.70146

Qian Li, Aihong Ji, Huan Shen, Guodong Qin, Binrui Wang

{"title":"The forewing-hindwing coupling of a black cicada Cryptotympana atrata (Hemiptera: Cicadidae): Functional morphology and kinematics.","authors":"Qian Li, Aihong Ji, Huan Shen, Guodong Qin, Binrui Wang","doi":"10.1111/1744-7917.70146","DOIUrl":"10.1111/1744-7917.70146","url":null,"abstract":"The wing structures and flapping dynamics of insects are diverse, which have important bionic implications for aircraft design. The black cicada, Cryptotympana atrata, which is a morphologically 4-winged insect, while its fore- and hindwings are coupled during flight. The functional morphology and kinematics in insects with coupled forewings-hindwings are still unclear. Further, although such wing coupling is common in nature, its impact on aerodynamic forces generated by flapping wings remains to be explored. In this study, we examine the micromorphology of the coupling structures on both fore- and hindwings of C. atrata. We analyze the kinematics and aerodynamics as well as variations in angle between the fore- and hindwings of C. atrata during tethered flight. The results show that fore- and hindwings are coupled through a hinge mechanism involving the rolled margin on the forewing and groove on the hindwing during flight. C. atrata follows a slender 0-shaped trajectory, and the dragged long trajectory line in pronation and supination is aimed to maintain stability. Due to the hinged coupling structure, there are significant changes in coupling angles during flight which influence wing twist, camber, and bending, ultimately enhancing aerodynamic forces by creating curved surfaces. This comprehensive study enhances our understanding of wing-coupling mechanisms and their functional significance for insects while providing valuable insights for future design and development of bionic coupled wings for flapping micro aerial vehicle systems.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2109-2122"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes. 不可胜选扶桑的遗传结构、扩散途径和向北扩张预测：中国不同景观的20年之旅。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-21 DOI: 10.1111/1744-7917.70133

Jun Huang, Chen-Di Yu, Shi-Zhen Wang, Jian-Dong Bao, Zhi-Tao Jiang, Wan-Ying Dong, Hong-Li Su, Li-Ming Chen, Farman Ullah, Shu-Xing Zhou, Xiao-Wei Li, Jin-Ming Zhang, Zhi-Jun Zhang, Yao-Bin Lu, Juan Zhang

{"title":"Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes.","authors":"Jun Huang, Chen-Di Yu, Shi-Zhen Wang, Jian-Dong Bao, Zhi-Tao Jiang, Wan-Ying Dong, Hong-Li Su, Li-Ming Chen, Farman Ullah, Shu-Xing Zhou, Xiao-Wei Li, Jin-Ming Zhang, Zhi-Jun Zhang, Yao-Bin Lu, Juan Zhang","doi":"10.1111/1744-7917.70133","DOIUrl":"10.1111/1744-7917.70133","url":null,"abstract":"The red imported fire ant, Solenopsis invicta, has invaded China for over two decades, advancing northwest from Guangdong to the Yangtze River coast at 30.8° N latitude. A decade since the last genetic analysis, this study revisits the species' genetic structure and invasion trajectory amidst intensifying selective pressures. Through mitochondrial DNA resequencing of 96 samples across 6 Chinese provinces, we identified 2 haplotypes (H5 and H22), with H22 predominating in Zhejiang, the northern limit of our study. Phylogenetic analysis suggests a likely invasion source from Guangdong and Fujian, with long-distance dispersal facilitated by the horticultural trade, particularly Cycas revoluta. Local dispersal rates varied significantly, with 40 m/year in northern Zhejiang and 118-186 m/year in Guangdong. A regression model (y = -303.2523 + 10 855.1813/x) forecasts a northern expansion threshold near southern Shandong. However, considering the bridgehead effect, repeated invasions, and climate change, the species' invasive range may extend further north.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"2179-2188"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

R-loop modulates odor-guided behavior in migratory locusts. R-loop调节迁徙蝗虫的气味引导行为。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-28 DOI: 10.1111/1744-7917.70134

Jipeng Liu, Lihan Zhao, Jiaqi Yu, Jing Qu, Zongyuan Ma

{"title":"R-loop modulates odor-guided behavior in migratory locusts.","authors":"Jipeng Liu, Lihan Zhao, Jiaqi Yu, Jing Qu, Zongyuan Ma","doi":"10.1111/1744-7917.70134","DOIUrl":"10.1111/1744-7917.70134","url":null,"abstract":"In neurodegenerative disorders, excessive R-loops affect DNA replication, transcription and genome stability. Olfaction impairment is an early indicator of these diseases. However, the function of R-loops in olfactory modulation is less well known. Migratory locusts show olfactory response to locust and plant odorants. Therefore, we chose this insect as a model to study the functions of R-loops in modulating olfaction in the central nervous system. The R-loop exists in the antenna and brain of this insect, and RNase H1 (RNH1), an enzyme that resolves the R-loop structure, is also expressed in these 2 tissues. Immunofluorescence analysis identifies the existence of R-loops in the brain, especially in mushroom bodies and antennal lobes, and RNH1 is also expressed in these 2 brain regions. RNH1 RNA interference (RNAi) knockdown does not affect the locomotion and body color of this insect, but results in the loss of olfactory responses to their own volatiles and plant odorants. Moreover, RNH1 deficiency results in increased R-loop levels in the protocerebrum and antennal lobes of the brain. RNH1 RNAi knockdown results in DNA damage and decreases nascent RNA synthesis in locust brains. This study reports that the increase in R-loop levels resulting from RNH1 knockdown may induce the loss of olfactory response of this insect to conspecific and plant odorants. This study is the first to show that excessive R-loops and RNH1 in the brain are involved in olfactory behavioral regulation. The identification of RNH1-mediated R-loop resolution will provide new insights into the functions of R-loops in modulating olfaction.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1882-1902"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144730193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR screening unravels genome-wide regulators of juvenile hormone signaling in Bombyx mori. CRISPR筛选揭示家蚕幼崽激素信号的全基因组调节因子。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-07-02 DOI: 10.1111/1744-7917.70103

Hao Sun, Chaojie Chen, Zijian Shi, Yue Liu, Ruolin Wang, Dan Liu, Tong Zhang, Sanyuan Ma, Ling Jia

{"title":"CRISPR screening unravels genome-wide regulators of juvenile hormone signaling in Bombyx mori.","authors":"Hao Sun, Chaojie Chen, Zijian Shi, Yue Liu, Ruolin Wang, Dan Liu, Tong Zhang, Sanyuan Ma, Ling Jia","doi":"10.1111/1744-7917.70103","DOIUrl":"10.1111/1744-7917.70103","url":null,"abstract":"Insect metamorphosis constitutes a sophisticated endocrine cascade governed by the dynamic interplay between juvenile hormone (JH) and 20-hydroxyecdysone (20E). Although JH has been established as a critical developmental regulator, its intracellular signaling mechanisms remain incompletely characterized. In this study, we implemented a genome-wide CRISPR screening strategy in the lepidopteran model Bombyx mori to systematically identify components of JH signaling. First, we developed a JH-responsive reporter cell line with EGFP expression under the control of JH-sensitive promoters, enabling real-time monitoring of pathway activation. Then we established a genome-scale CRISPR knockout library specifically optimized for silkworm cells, and performed screening through integration of high-throughput flow cytometry and next-generation sequencing. Systematic analysis revealed a total of 387 candidate genes that significantly modulated JH signaling activity (231 enriched vs. 156 depleted). Functional annotation clustering revealed predominant enrichment in membrane biogenesis processes and small molecule metabolism. It is worth noting that through integrated genomic screening and experimental verification, we found that the tyrosine kinase receptor BmALK is an important regulatory node for JH signal transduction. This study establishes the first comprehensive genetic landscape of JH signaling in a holometabolous insect, providing a robust platform for functional mining of JH signaling pathways, and molecular evidence for membrane-associated JH signal transduction in Lepidoptera.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1738-1752"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic-developmental crosstalk: BmDHFR-mediated Hedgehog signaling orchestrates organ development and lipid homeostasis in Bombyx mori. 代谢-发育串：bmdhfr介导的刺猬信号调控家蚕的器官发育和脂质稳态。

IF 3.7 1区农林科学

Insect Science Pub Date : 2026-10-01 Epub Date: 2025-08-11 DOI: 10.1111/1744-7917.70149

Dan Liang, Song Jiang, Mengjun Xu, Yulong Yu, Daoming Wang, Yanjiao Huang, Xiaming Shi, Yan Meng

{"title":"Metabolic-developmental crosstalk: BmDHFR-mediated Hedgehog signaling orchestrates organ development and lipid homeostasis in Bombyx mori.","authors":"Dan Liang, Song Jiang, Mengjun Xu, Yulong Yu, Daoming Wang, Yanjiao Huang, Xiaming Shi, Yan Meng","doi":"10.1111/1744-7917.70149","DOIUrl":"10.1111/1744-7917.70149","url":null,"abstract":"Dihydrofolate reductase (DHFR), a pivotal enzyme in folate metabolism and DNA synthesis, is a well-established cancer therapeutic target. However, its non-canonical roles in developmental signaling and evolutionary-conserved functions in non-mammalian species remain poorly characterized. Using the lepidopteran model Bombyx mori, this study uncovered the pleiotropic functions of BmDHFR in midgut proliferation and lipid homeostasis through dual metabolic and Hedgehog (Hh) signaling regulation. Spatiotemporal profiling revealed ubiquitous BmDHFR expression during larval development. RNA interference-mediated BmDhfr knockdown suppressed midgut cell proliferation, validating its conserved role in DNA replication via thymidylate (deoxythymidine monophosphate [dTMP]) synthesis, while unexpectedly up-regulating the adipogenic marker BmAP2 and inducing lipid droplet hypertrophy-contrasting mammalian white adipocyte differentiation. Mechanistically, BmDHFR directly interacts with silkworm suppressor of fused (BmSUFU), the core suppressor of Hh signaling, forming a \"metabolism-signaling-cell fate\" axis that bridges folate metabolism to Hh pathway regulation. Cross-species comparisons highlight evolutionary divergence: Hh signaling inhibits insect adipogenesis but regulates mammalian adipocyte differentiation, suggesting adaptive remodeling of DHFR-Hh networks. This study advances the functional characterization of BmDHFR, elucidating evolutionary conservation and species-specific divergence in metabolic enzyme pleiotropy, and lays a foundation for drug screening and disease research based on insect models.","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":"1966-1978"},"PeriodicalIF":3.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0