Deterrence and behavioral mode of coconut oil-derived free fatty acids on Zeugodacus cucurbitae oviposition.

IF 2.9 1区 农林科学 Q1 ENTOMOLOGY
Vijaya Movva, Junwei Zhu, Amy Roda, Paul Kendra, Xiangbing Yang, Kevin Cloonan, Jia-Wei Tay, Dong H Cha
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引用次数: 0

Abstract

Previous studies have shown oviposition deterring properties of 8 coconut free fatty acid (CFFA) compounds on fruit flies with different key deterrent components for different species. Here we evaluated oviposition deterrence of CFFA using laboratory 2-choice bioassays against Zeugodacus cucurbitae, determined key-bioactive deterrent compounds, and evaluated their behavioral mode. Unlike other reported fruit fly species, CFFA mixture increased Z. cucurbitae oviposition when directly applied on an oviposition substrate. When tested individually in subsequent tests, 4 compounds (caprylic, capric, oleic, and linoleic acids) significantly reduced the oviposition ("negative-compounds"), 1 compound (stearic acid) had no effect ("neutral-compound"), and 3 compounds (lauric, myristic, and palmitic acids) stimulated the oviposition ("positive-compounds"). The 4-component negative-compound blend was effective at reducing oviposition. However, adding stearic acid to the 4-component blend (5-component blend, 5c) further reduced oviposition. Adding any of the positive-compounds to the 5c resulted in loss of oviposition deterrence, suggesting the 5c as the key deterrent component blend. The blend was also effective in no-choice assays and when applied on cucumbers, a preferred host of Z. cucurbitae. When given a choice, Z. cucurbitae made 48.5% fewer visits, spent 39% less time, and oviposited 88.2% fewer eggs per min on 5c treated pumpkin agar than on control agar, suggesting that the 5c blend has both spatial repellency and contact deterrence. Given that all compounds are registered food additives and generally regarded as safe, this blend has potential application in behavioral control strategies, such as push-pull, to protect host fruit against Z. cucurbitae.

椰子油游离脂肪酸对葫芦蝇产卵的威慑和行为模式
以往的研究表明,8 种椰子游离脂肪酸(CFFA)化合物对果蝇具有产卵阻遏特性,不同种类的果蝇具有不同的关键阻遏成分。在此,我们利用实验室二选一生物测定法评估了 CFFA 对葫芦斑潜蝇(Zeugodacus cucurbitae)的产卵阻遏作用,确定了关键生物活性阻遏化合物,并评估了它们的行为模式。与其他报道的果蝇物种不同,CFFA 混合物直接施用在产卵基质上时,会增加葫芦蝇的产卵量。在随后的单独测试中,4 种化合物(辛酸、癸酸、油酸和亚油酸)显著降低了产卵量("阴性化合物"),1 种化合物(硬脂酸)没有影响("中性化合物"),3 种化合物(月桂酸、肉豆蔻酸和棕榈酸)刺激了产卵量("阳性化合物")。4 组分的阴性化合物混合物能有效减少卵子的排出。然而,在 4 组分混合物(5 组分混合物,5c)中添加硬脂酸会进一步减少卵生。在 5c 中添加任何一种阳性化合物都会导致卵产阻遏作用的丧失,这表明 5c 是关键的阻遏成分混合物。该混合物在无选择试验中以及在葫芦科害虫的首选宿主黄瓜上施用时也很有效。与对照琼脂相比,在经过 5c 处理的南瓜琼脂上,葫芦蝇的光顾次数减少了 48.5%,花费的时间减少了 39%,每分钟产卵量减少了 88.2%,这表明 5c 混合物同时具有空间驱避性和接触阻遏性。鉴于所有化合物都是注册食品添加剂,而且普遍认为是安全的,这种混合物有可能应用于行为控制策略,如推拉法,以保护寄主果实免受葫芦瓢虫的危害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Insect Science
Insect Science 生物-昆虫学
CiteScore
7.80
自引率
5.00%
发文量
1379
审稿时长
6.0 months
期刊介绍: Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
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