Insect Molecular Biology最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 3","pages":"310-322"},"PeriodicalIF":2.3,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148091280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 3","pages":"270-283"},"PeriodicalIF":2.3,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148064883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 3","pages":"284-295"},"PeriodicalIF":2.3,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148098585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 3","pages":"296-309"},"PeriodicalIF":2.3,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 3","pages":"257-269"},"PeriodicalIF":2.3,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148070374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformer is involved in female sex determination in Bemisia tabaci","authors":"Yating Liu,&nbsp;Wenlu Wang,&nbsp;Yina Wang,&nbsp;Xuguo Zhou,&nbsp;Wen Xie,&nbsp;Youjun Zhang","doi":"10.1111/imb.70000","DOIUrl":"10.1111/imb.70000","url":null,"abstract":"<p>Sex determination is one of the fundamental questions in developmental and evolutionary biology. While the upstream regulatory mechanisms governing somatic sex determination in insects are highly divergent, the downstream <i>transformer</i> (<i>tra</i>) + <i>transformer2</i> (<i>tra2</i>) → <i>doublesex</i> (<i>dsx</i>) pathway is relatively conserved. In the sweet potato whitefly, <i>Bemisia tabaci</i>, a global invasive pest, <i>tra2</i> and <i>dsx</i> have been previously characterised, revealing their critical role in male genitalia formation. However, the role of <i>tra</i> in <i>B. tabaci</i> sex determination has remained unclear. Here, we characterised <i>Bttra</i>, the <i>B. tabaci transformer</i>, and identified multiple TRA/TRA-2 binding sites and several splice variants. A glutathione S-transferase (GST) pull-down assay confirmed direct interactions between BtTRA and BtTRA2 in vitro. Functional analysis through <i>Bttra</i> silencing demonstrated its regulatory impact on <i>Btdsx</i> expression and its essential role in female development. Our combined findings provide new insights into the function of <i>tra</i> in <i>B. tabaci</i> sex determination, highlighting variation in sex determination among haplodiploid insects. Moreover, this study identifies potential molecular targets for the future management of this devastating insect pest.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 1","pages":"23-33"},"PeriodicalIF":2.3,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut-associated bacteria and their roles in wood digestion of saproxylic insects: The case study of flower chafer larvae","authors":"Worarat Kruasuwan,&nbsp;Tantip Arigul,&nbsp;John Tyson Munnoch,&nbsp;Pumin Nutaratat,&nbsp;Nut Songvorawit","doi":"10.1111/imb.70010","DOIUrl":"10.1111/imb.70010","url":null,"abstract":"<p><i>Protaetia acuminata</i> (Fabricius, 1775) (Coleoptera: Scarabaeidae) is widely distributed throughout Southeast Asia and plays a significant role in nutrient cycling by facilitating the decomposition of woody materials, a process that likely relies heavily on the contribution of symbiotic bacteria within their digestive system. However, their gut bacteria have not been thoroughly studied. By using V3-V4 amplicon sequencing, it was revealed that the midgut (MG) of <i>Pr. acuminata</i> larvae and fermented sawdust after rearing (FSD) share a similar microbial community, predominantly composed of Proteobacteria and Actinobacteriota, as well as functional genes associated with cellulolysis, nitrogen respiration, nitrate reduction and aerobic chemoheterotrophy. In contrast, the bacterial community in the hindgut (HG) was distinctly different, with anaerobic respiration being the dominant metabolic process. <i>Agromyces</i>, <i>Altererythrobacter</i>, <i>Bacillus</i>, <i>Cellulomonas</i>, <i>Lysinibacillus</i>, <i>Pseudoxanthomonas</i> and the family Promicromonosporaceae were the most common genera in MG, HG and FSD samples. The culture-based isolation method yielded 67 isolates from the larvae, with gram-positive bacteria predominating in HG and MG, whereas gram-negative bacteria were primarily found in the FSD. These microorganisms produce a range of lignocellulolytic enzymes including β-endoglucanase, laccase and xylanase that enable the beetles to digest their plant-based diet efficiently and also involve many biochemical pathways relating to biogeochemical cycling. Our results provide valuable insights into the gut-associated <i>Pr. acuminata</i> flower chafer larvae and could serve as a basis and reservoir for future studies on lignocellulolytic enzyme-producing bacteria.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 1","pages":"91-103"},"PeriodicalIF":2.3,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone acetyltransferase p300/CBP regulates reproductive diapause via the juvenile hormone pathway in the cabbage beetle, Colaphellus bowringi","authors":"Hao-Min An,&nbsp;Yi-Ke Zhang,&nbsp;Qian-Lu Bai,&nbsp;Wen Liu,&nbsp;Xiao-Ping Wang","doi":"10.1111/imb.70007","DOIUrl":"10.1111/imb.70007","url":null,"abstract":"<p>Juvenile hormone (JH) serves as a pivotal endocrine regulator of reproductive diapause (also known as adult diapause) in insects, though the molecular mechanisms underlying JH signalling remain poorly characterised. In this study, we investigate the roles of the histone acetyltransferase p300/CBP in regulating reproductive diapause through JH signalling in the cabbage beetle <i>Colaphellus bowringi</i>. RNA interference-mediated knockdown of <i>p300/CBP</i> in reproductive females induced diapause-like phenotypes, including arrested ovarian maturation, suppressed vitellogenesis, increased lipid accumulation and upregulation of diapause-associated genes. These effects were accompanied by downregulation of key JH biosynthetic enzymes and signalling components. Although exogenous JH application decreased lipid accumulation, it failed to rescue ovarian development. Similar results were observed in diapause females following <i>p300/CBP</i> knockdown: JH analog treatment reduced lipid accumulations but did not restore ovarian development. Collectively, these findings demonstrate that <i>p300/CBP</i> regulates photoperiodic reproductive diapause by modulating both JH biosynthesis and signal transduction pathways in <i>C. bowringi</i>. Our results establish p300/CBP as a critical epigenetic mediator of JH-mediated reproductive diapause and provide new insights into the molecular mechanisms underlying environmental adaptation in insects.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 1","pages":"66-78"},"PeriodicalIF":2.3,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of DNA methyltransferases (DNMTs) in Spodoptera frugiperda (Sf9) cells following AcMNPV infection, and its effects on the virus-cell interaction","authors":"Naeime Karamipour,&nbsp;Ali Asghar Talebi,&nbsp;Yaghoub Fathipour,&nbsp;Sassan Asgari,&nbsp;Mohammad Mehrabadi","doi":"10.1111/imb.70006","DOIUrl":"10.1111/imb.70006","url":null,"abstract":"<p>Baculoviruses have been used to control different insect pests, but further research is needed to understand the mechanisms of their interactions with insect hosts. In this study, the importance of DNA methyltransferases (DNMTs) was investigated in <i>Spodoptera frugiperda</i> Sf9 cells following baculovirus infection. To do this, Sf9 cells were infected with Autographa californica nucleopolyhedrovirus (AcMNPV) and the transcript levels of DNMT1 and DNMT2 were quantified. The expression levels of both DNMTs were upregulated during AcMNPV infection, suggesting induction of the pathway during infection. To determine whether these genes function during virus infection, we treated Sf9 cells with the inhibitor of DNMTs, 5-azacytidine (5-AZA), and 24 h later, the cells were infected with AcMNPV. Quantitative PCR results showed that the viral DNA replication was significantly reduced in the treated cells compared with the control untreated cells. We also showed that 5-AZA modulates antiviral pathways in Sf9 cells. The expression levels of Toll and immune-deficiency (IMD) pathways genes (i.e., <i>Relish</i> and <i>Dorsal</i>) were increased at 24 h post AcMNPV infection, while the expression levels of <i>Gloverin</i> and <i>Defensin</i> antimicrobial peptides (AMPs) were downregulated. In the 5-AZA treated cells, however, the expression levels of both AMPs were elevated during AcMNPV infection. We also assessed the effect of 5-AZA on short interfering RNA and microRNA pathways and found that <i>Dicer 2</i> expression level was increased in the 5-AZA treated cells, while the transcript levels of other key genes of the two pathways remained unchanged. Together, our results highlight the contribution of DNA methyltransferase activity in the immunity of Sf9 cells against AcMNPV infection through modulation of antiviral immune responses and shed more light on the molecular mechanisms underlying baculovirus-insect cell interactions.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 1","pages":"14-22"},"PeriodicalIF":2.3,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of IR25a on electrophysiology and reproductive behaviour in the oriental fruit fly, Bactrocera dorsalis","authors":"Qian Qian,&nbsp;Jinxi Yuan,&nbsp;Jie Zhang,&nbsp;Qi Wang,&nbsp;Shanchun Yan,&nbsp;Wei Liu,&nbsp;Guirong Wang","doi":"10.1111/imb.70005","DOIUrl":"10.1111/imb.70005","url":null,"abstract":"<p>Behavioural manipulation techniques, particularly attractants, have been widely adopted as environmentally friendly approaches to manage <i>Bactrocera dorsalis</i>. These approaches primarily target odorant receptors (ORs), key molecular components in the pest's chemotactic pathways, to guide pests towards traps or lethal stimuli, offering a sustainable and effective alternative to chemical pesticides. However, pest behaviour extends beyond chemotaxis. Crucial behaviours such as mating, oviposition and defence also directly influence population dynamics, underscoring the need to investigate molecular targets that regulate diverse behavioural processes. IR25a, a co-receptor of ionotropic receptors (IRs), exhibits functional versatility by detecting diverse signals, including both volatile and non-volatile compounds and environmental cues (e.g., temperature, humidity, acidity). These characteristics make <i>IR25a</i> a promising candidate target that influences various behavioural processes. In this study, CRISPR/Cas9 was employed to generate a <i>BdorIR25a</i> knockout homozygous strain (<i>BdorIR25a</i>^<i>-/-</i>). Its effects on various aspects of pest behaviour were systematically investigated to evaluate its potential as a molecular target for behavioural modification. Results showed that <i>BdorIR25a</i> was highly expressed in pupal and adult stages, particularly in antennae and mouthparts. Knockouts exhibited impaired responsiveness to specific chemicals, especially dimethylamine. Additionally, mutants displayed reduced courtship wing vibration, pheromone responsiveness and mating success. Oviposition rates and egg-laying numbers were significantly reduced in females. Importantly, knockouts had no detectable effects on growth or survival, confirming BdorIR25a's primary role in behavioural modification. In conclusion, our findings identify <i>BdorIR25a</i> as a key molecular target with strong potential for manipulating diverse behavioural processes, providing a new perspective for pest control strategies. Future studies should focus on identifying <i>IR25a</i>-associated <i>IRs</i> and other molecular targets within its linked regions to clarify their respective roles in modulating critical behavioural traits. These studies could provide detailed insights into the molecular mechanisms of behaviour manipulation, advancing the development of innovative and targeted pest control strategies.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 1","pages":"1-13"},"PeriodicalIF":2.3,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144872929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}