CRISPR/Cas9 介导的全球害虫--秋军虫(Spodoptera frugiperda)腹部-B 同源基因的敲除。

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xiao-Guang Liu, Te Zhao, Qi-Qi Qiu, Cong-Ke Wang, Tian-Liang Li, Xiao-Long Liu, Li Wang, Qin-Qin Wang, Lin Zhou
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引用次数: 0

摘要

同源复合体(Hox)基因在决定两栖动物前后轴的节段特征和附肢形态方面起着至关重要的作用。最近的研究已扩展到农业害虫,如秋季军虫(FAW),学名 Spodoptera frugiperda J. E. Smith(鳞翅目:夜蛾科),它严重威胁全球农业生产力。然而,hox基因Sfabd-B在FAW中的具体作用仍有待探索。本研究利用实时定量聚合酶链式反应(qRT-PCR)研究了Sfabd-B在不同发育阶段的不同组织中的时空表达模式。此外,我们还利用CRISPR/Cas9技术探索了Sfabd-B基因在一窝蜂基因组中的潜在功能。与野生型个体相比,幼虫突变体的表型可分为三个亚组,即后腹部足突过多、节段融合导致足突不足以及后腹部节段偏离。重要的是,在蛹期和成虫期,雌雄个体的突变体表型也存在明显差异。值得注意的是,与野生型相比,雄性突变体的交配器官和雌性突变体的产卵器中,断头截瘫(dpp)基因和角质蛋白12(cp 12)基因的表达水平都明显下降。这些发现凸显了Sfabd-B在生殖道模式化中的重要性,为改善遗传控制提供了一个潜在的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CRISPR/Cas9-mediated knockout of the abdominal-B homeotic gene in the global pest, fall armyworm (Spodoptera frugiperda).

The Homeotic complex (Hox) genes play a crucial role in determining segment identity and appendage morphology in bilaterian animals along the antero-posterior axis. Recent studies have expanded to agricultural pests such as fall armyworm (FAW), scientifically known as Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae), which significantly threatens global agricultural productivity. However, the specific role of the hox gene Sfabd-B in FAW remains unexplored. This research investigates the spatial and temporal expression patterns of Sfabd-B in various tissues at different developmental stages using quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, we explored the potential function of the Sfabd-B gene located in the FAW genome using CRISPR/Cas9 technology. The larval mutant phenotypes can be classified into three subgroups as compared with wild-type individuals, that is, an excess of pedis in the posterior abdomen, deficient pedis due to segmental fusion and deviations in the posterior abdominal segments. Importantly, significant differences in mutant phenotypes between male and female individuals were also evident during the pupal and adult phases. Notably, both the decapentaplegic (dpp) and cuticular protein 12 (cp 12) genes displayed a substantial marked decrease in expression levels in the copulatory organ of male mutants and the ovipositor of female mutants compared with the wild type. These findings highlight the importance of Sfabd-B in genital tract patterning, providing a potential target for improving genetic control.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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