{"title":"Role of TRPV channels in texture discrimination during Bactrocera dorsalis egg-laying behaviour.","authors":"Zhong-Nian Huang, Feng-Yi Guo, Zi-Yuan Li, Yong-Yue Lu, Yi-Xiang Qi","doi":"10.1111/imb.12970","DOIUrl":null,"url":null,"abstract":"<p><p>Deciding where to lay an egg is critical for the survival of insects' offspring. Compared with our understanding of the chemosensory assessment of egg-laying sites, the mechanisms of texture detection are largely unknown. Here, we show that Bactrocera dorsalis, a notoriously agricultural pest laying its eggs within ripening fruits, can discriminate substrate texture during the egg-laying process. Exposure to drugs targeting transient receptor potential vanilloid (TRPV) mechanosensory channels abolished their oviposition preference for hard textures. BdorNan and BdorIav are two members of the TRPV subfamily, and their transcripts were detected in the labellum, the foreleg tarsi and the ovipositor. Then, we successfully obtained knockout strains of each gene using the CRISPR/Cas9 technique. The results showed that BdorNan is required for the discrimination of stiffness difference. BdorIav knockout had no significant effect on the ability of B. dorsalis to choose harder substrates. Our study thus reveals that BdorNan plays a substantial role in the texture assessment of egg-laying behaviour in B. dorsalis.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/imb.12970","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Deciding where to lay an egg is critical for the survival of insects' offspring. Compared with our understanding of the chemosensory assessment of egg-laying sites, the mechanisms of texture detection are largely unknown. Here, we show that Bactrocera dorsalis, a notoriously agricultural pest laying its eggs within ripening fruits, can discriminate substrate texture during the egg-laying process. Exposure to drugs targeting transient receptor potential vanilloid (TRPV) mechanosensory channels abolished their oviposition preference for hard textures. BdorNan and BdorIav are two members of the TRPV subfamily, and their transcripts were detected in the labellum, the foreleg tarsi and the ovipositor. Then, we successfully obtained knockout strains of each gene using the CRISPR/Cas9 technique. The results showed that BdorNan is required for the discrimination of stiffness difference. BdorIav knockout had no significant effect on the ability of B. dorsalis to choose harder substrates. Our study thus reveals that BdorNan plays a substantial role in the texture assessment of egg-laying behaviour in B. dorsalis.
期刊介绍:
Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins.
This includes research related to:
• insect gene structure
• control of gene expression
• localisation and function/activity of proteins
• interactions of proteins and ligands/substrates
• effect of mutations on gene/protein function
• evolution of insect genes/genomes, especially where principles relevant to insects in general are established
• molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations
• gene mapping using molecular tools
• molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects
Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).