Molecular characterization of a novel thioredoxin-related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause.

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qiaozhi Luo, Jianjun Mao, Yuyan Li, Mengqing Wang, Lisheng Zhang, Zhongjian Shen
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引用次数: 0

Abstract

Protein disulphide isomerase (PDI) possesses disulphide isomerase, oxidoreductase and molecular chaperone activities, and is involved in regulating various physiological processes. However, there are few studies on the function in insect diapause. In this study, we cloned one novel member PDI family (TMX3, thioredoxin-related transmembrane protein 3) in Arma chinensis. The AcTMX3 encodes 426 amino acids that contains a predicted N-terminal signal sequence, a thioredoxin-like domain with the CXXC active site and a potential transmembrane region, which are typical sequence features of TMX3. RT-qPCR results showed that AcTMX3 was mainly expressed in the head under non-diapause conditions, while AcTMX3 was highly expressed in the fat body (central metabolic organ) under diapause conditions. Moreover, temporal expression profile showed that compared with non-diapause conditions, diapause conditions significantly induced AcTMX3 expression, and the expression of AcTMX3 was enhanced at 15°C. Silencing AcTMX3 in A. chinensis significantly inhibited the expression of antioxidant genes (AcTrx2 and AcTrx-like), increased the content of H2O2 and ascorbate and reduced the survival rate of A. chinensis under diapause conditions. Our results suggested that AcTMX3 played an important role in the resistance of A. chinensis to oxidative stress under diapause conditions.

新型硫氧还蛋白相关跨膜蛋白基因 AcTMX3 的分子特征,该基因在 Arma chinensis diapause 的抗氧化防御中发挥重要作用。
蛋白二硫异构酶(PDI)具有二硫异构酶、氧化还原酶和分子伴侣蛋白活性,参与调节多种生理过程。然而,有关其在昆虫休眠中功能的研究很少。本研究克隆了一种新的 PDI 家族成员(TMX3,硫代氧化还蛋白相关跨膜蛋白 3)。AcTMX3编码426个氨基酸,包含一个预测的N端信号序列、一个具有CXXC活性位点的硫代毒素样结构域和一个潜在的跨膜区,这些都是TMX3的典型序列特征。RT-qPCR 结果表明,在非休眠状态下,AcTMX3 主要在头部表达,而在休眠状态下,AcTMX3 在脂肪体(中央代谢器官)中高表达。此外,时间表达谱显示,与非休眠条件相比,休眠条件显著诱导 AcTMX3 的表达,且 AcTMX3 的表达在 15°C 时增强。沉默AcTMX3可明显抑制抗氧化基因(AcTrx2和AcTrx-like)的表达,增加H2O2和抗坏血酸的含量,降低休眠状态下 chinensis的存活率。我们的研究结果表明,AcTMX3 在减产条件下盐池蛙抵抗氧化胁迫的过程中发挥了重要作用。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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