Insect Molecular Biology最新文献

{"title":"CRISPR/Cas9-mediated Nap knockout affects female reproduction and egg shape in Bombyx mori","authors":"Xingyu Liu,&nbsp;Liying Zhang,&nbsp;Ning Zhang,&nbsp;Kai Li,&nbsp;Peter B. Mater,&nbsp;Lin He","doi":"10.1111/imb.12937","DOIUrl":"10.1111/imb.12937","url":null,"abstract":"<p>Insect reproductive capacity can affect effective pest control and infertility studies and has become an important focus in recent molecular genetic research. Nucleosome assembly protein (<i>Nap</i>) is highly conserved across multiple species and is involved in forming the sperm nucleus in many species. We used clustered regularly interspaced palindromic repeats/Cas9 technology to knockout <i>BmNap</i> in <i>Bombyx mori</i> and observed that the mutations caused female infertility, whereas male fertility was not affected. <i>BmNap</i> mutants grew and mated normally; however, female mutants laid smaller eggs that could not be fertilised and did not hatch. In addition, female sterility produced by the mutation could be inherited stably via male mutants; therefore, <i>Nap</i> could be used as a potential target for lepidopteran pest control through population regulation. In the current study, we elucidated a new function of <i>BmNap</i>, increased the understanding of the oogenesis regulation network in Lepidoptera and promoted the development of insect sterility technologies.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"722-731"},"PeriodicalIF":2.3,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141544747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drivers of genomic diversity and phenotypic development in early phases of domestication in Hermetia illucens","authors":"Kelvin L. Hull,&nbsp;Matthew P. Greenwood,&nbsp;Melissa Lloyd,&nbsp;Marissa Brink-Hull,&nbsp;Aletta E. Bester-van der Merwe,&nbsp;Clint Rhode","doi":"10.1111/imb.12940","DOIUrl":"10.1111/imb.12940","url":null,"abstract":"<p>The black soldier fly (BSF), <i>Hermetia illucens</i>, has the ability to efficiently bioremediate organic waste into usable bio-compounds. Understanding the impact of domestication and mass rearing on fitness and production traits is therefore important for sustainable production. This study aimed to assess patterns of genomic diversity and its association to phenotypic development across early generations of mass rearing under two selection strategies: selection for greater larval mass (SEL lines) and no direct artificial selection (NS lines). Genome-wide single nucleotide polymorphism (SNP) data were generated using 2bRAD sequencing, while phenotypic traits relating to production and population fitness were measured. Declining patterns of genomic diversity were observed across three generations of captive breeding, with the lowest diversity recorded for the F3 generation of both selection lines, most likely due to founder effects. The SEL cohort displayed statistically significantly greater larval weight com the NS lines with pronounced genetic and phenotypic directional changes across generations. Furthermore, lower genetic and phenotypic diversity, particularly for fitness traits, were evident for SEL lines, illustrating the trade-off between selecting for mass and the resulting decline in population fitness. SNP-based heritability was significant for growth, but was low or non-significant for fitness traits. Genotype–phenotype correlations were observed for traits, but individual locus effect sizes where small and very few of these loci demonstrated a signature for selection. Pronounced genetic drift, due to small effective population sizes, is likely overshadowing the impacts of selection on genomic diversity and consequently phenotypic development. The results hold particular relevance for genetic management and selective breeding for BSF in future.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"756-776"},"PeriodicalIF":2.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12940","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in understanding of the mechanisms of RNA interference in insects.","authors":"Jinmo Koo, Subba Reddy Palli","doi":"10.1111/imb.12941","DOIUrl":"https://doi.org/10.1111/imb.12941","url":null,"abstract":"<p><p>We highlight the recent 5 years of research that contributed to our understanding of the mechanisms of RNA interference (RNAi) in insects. Since its first discovery, RNAi has contributed enormously as a reverse genetic tool for functional genomic studies. RNAi is also being used in therapeutics, as well as agricultural crop and livestock production and protection. Yet, for the wider application of RNAi, improvement of its potency and delivery technologies is needed. A mechanistic understanding of every step of RNAi, from cellular uptake of RNAi trigger molecules to targeted mRNA degradation, is key for developing an efficient strategy to improve RNAi technology. Insects provide an excellent model for studying the mechanism of RNAi due to species-specific variations in RNAi efficiency. This allows us to perform comparative studies in insect species with different RNAi sensitivity. Understanding the mechanisms of RNAi in different insects can lead to the development of better strategies to improve RNAi and its application to manage agriculturally and medically important insects.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of poly (ADP-ribose) polymerase 1 (PARP1) on silk proteins in the silkworm, Bombyx mori","authors":"Mingke Wu,&nbsp;Hao Sun,&nbsp;Aoming Wang,&nbsp;Junjie Lao,&nbsp;Dan Liu,&nbsp;Chaojie Chen,&nbsp;Yan Zhang,&nbsp;Qingyou Xia,&nbsp;Sanyuan Ma","doi":"10.1111/imb.12938","DOIUrl":"10.1111/imb.12938","url":null,"abstract":"<p>Animal silk is economically important, while silk secretion is a complex and subtle mechanism regulated by many genes. We identified the poly (ADP-ribose) polymerase (<i>PARP1</i>) gene of the silkworm and successfully cloned its coding sequence (CDS) sequence. Using clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) technology, we screened single guide RNA (sgRNA) with high knockout efficiency by cellular experiments and obtained <i>PARP1</i> mutants by knocking out the <i>PARP1</i> gene of the silkworm at the individual level. We found that the mutants mainly exhibited phenotypes such as smaller cocoon size and reduced cocoon shell rate than the wild type. We also detected the expression of silk protein genes in the mutant by quantitative real-time PCR (qPCR) and found that the expression of some silk protein genes was slightly down-regulated. Meanwhile, together with the results of transcriptomic analysis, we hypothesized that <i>PARP1</i> may affect the synthesis of silk proteins, resulting in their failure to function properly. Our study may provide an important reference for future in-depth refinement of the molecular mechanism of silk protein expression in silk-producing animals, as well as a potential idea for future development of molecular breeding lines of silkworms to improve silk production.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"732-743"},"PeriodicalIF":2.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the UDP-glycosyltransferase UGT33D1 in silkworm Bombyx mori","authors":"Feifei Zhu,&nbsp;Jinying Han,&nbsp;Jingdie Hong,&nbsp;Fuchuan Cai,&nbsp;Qi Tang,&nbsp;Qian Yu,&nbsp;Shangshang Ma,&nbsp;Xiaoyong Liu,&nbsp;Shuhao Huo,&nbsp;Keping Chen","doi":"10.1111/imb.12935","DOIUrl":"10.1111/imb.12935","url":null,"abstract":"<p>Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are important metabolizing enzymes functioning by adding a sugar moiety to a small lipophilic substrate molecule and play critical roles in drug/toxin metabolism for all realms of life. In this study, the silkworm <i>Bombyx mori</i> UGT33D1 gene was characterized in detail. UGT33D1 was found localized in the endoplasmic reticulum (ER) compartment just like other animal UGTs and was mainly expressed in the silkworm midgut. We first reported that UGT33D1 was important to BmNPV infection, as silencing UGT33D1 inhibited the BmNPV infection in silkworm BmN cells, while overexpressing the gene promoted viral infection. The molecular pathways regulated by UGT33D1 were analysed via transcriptome sequencing upon UGT33D1 knockdown, highlighting the important role of the gene in maintaining a balanced oxidoreductive state of the organism. In addition, proteins that physically interact with UGT33D1 were identified through immunoprecipitation and mass spectrometry analysis, which includes tubulin, elongation factor, certain ribosomal proteins, histone proteins and zinc finger proteins that had been previously reported for human UGT-interacting proteins. This study provided preliminary but important functional information on UGT33D1 and is hoped to trigger deeper investigations into silkworm UGTs and their functional mechanisms.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"697-707"},"PeriodicalIF":2.3,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and proteomic elucidation of Z chromosome dosage compensation in Helicoverpa armigera","authors":"Zhongyuan Deng,&nbsp;Yakun Zhang,&nbsp;Xingcheng Xie,&nbsp;Huihui Li,&nbsp;Han Guo,&nbsp;Xinzhi Ni,&nbsp;Xianchun Li","doi":"10.1111/imb.12939","DOIUrl":"10.1111/imb.12939","url":null,"abstract":"<p>Transcriptomic data have been used to study sex chromosome dosage compensation (SCDC) in approximately 10 Lepidoptera ZW species, yielding a consensus compensation pattern of Z <span></span><math>\u0000 <mrow>\u0000 <mo>≈</mo>\u0000 <mi>ZZ</mi>\u0000 <mo>&lt;</mo>\u0000 <mi>AA</mi>\u0000 <mo>.</mo>\u0000 </mrow></math> It remains unclear whether this compensation pattern holds when examining more Lepidoptera ZW species and/or using proteomic data to analyse SCDC. Here we combined transcriptomic and proteomic data as well as transcriptional level of six individual Z genes to reveal the SCDC pattern in <i>Helicoverpa armigera</i>, a polyphagous lepidopteran pest of economic importance. Transcriptomic analysis showed that the Z chromosome expression of <i>H. armigera</i> was balanced between male and female but substantially reduced relative to autosome expression, exhibiting an SCDC pattern of Z <span></span><math>\u0000 <mrow>\u0000 <mo>≈</mo>\u0000 <mi>ZZ</mi>\u0000 <mo>&lt;</mo>\u0000 <mi>AA</mi>\u0000 </mrow></math>. When using <i>H. amigera</i> midgut proteomic data, the SCDC pattern of this species changed from Z <span></span><math>\u0000 <mrow>\u0000 <mo>≈</mo>\u0000 <mi>ZZ</mi>\u0000 <mo>&lt;</mo>\u0000 <mi>AA</mi>\u0000 </mrow></math> at transcriptomic level to Z = ZZ = AA at the proteomic level. RT-qPCR analysis of transcript abundance of six Z genes found that compensation for each Z gene could vary from no compensation to overcompensation, depending on the individual genes and tissues tested. These results demonstrate for the first time the existence of a translational compensation mechanism, which is operating in addition to a translational mechanism, such as has been reported in other lepidopteran species. And the transcriptional compensation mechanism functions to accomplish Z chromosome dosage balance between the sexes (M = F on the Z chromosome), whereas the translation compensation mechanism operates to achieve dosage compensation between Z chromosome and autosome (Z = AA).</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"744-755"},"PeriodicalIF":2.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA methylation machinery is involved in development and reproduction in the viviparous pea aphid (Acyrthosiphon pisum)","authors":"Kane Yoon,&nbsp;Stephanie Williams,&nbsp;Elizabeth J. Duncan","doi":"10.1111/imb.12936","DOIUrl":"10.1111/imb.12936","url":null,"abstract":"<p>Epigenetic mechanisms, such as DNA methylation, have been proposed to mediate plastic responses in insects. The pea aphid (<i>Acyrthosiphon pisum</i>), like the majority of extant aphids, displays cyclical parthenogenesis - the ability of mothers to switch the reproductive mode of their offspring from reproducing parthenogenetically to sexually in response to environmental cues. The pea aphid genome encodes two paralogs of the de novo DNA methyltransferase gene, <i>dnmt3a</i> and <i>dnmt3x</i>. Here we show, using phylogenetic analysis, that this gene duplication event occurred at least 150 million years ago, likely after the divergence of the lineage leading to the Aphidomorpha (phylloxerans, adelgids and true aphids) from that leading to the scale insects (Coccomorpha) and that the two paralogs are maintained in the genomes of all aphids examined. We also show that the mRNA of both <i>dnmt3</i> paralogs is maternally expressed in the viviparous aphid ovary. During development both paralogs are expressed in the germ cells of embryos beginning at stage 5 and persisting throughout development. Treatment with 5-azactyidine, a chemical that generally inhibits the DNA methylation machinery, leads to defects of oocytes and early-stage embryos and causes a proportion of later stage embryos to be born dead or die soon after birth. These phenotypes suggest a role for DNA methyltransferases in reproduction, consistent with that seen in other insects. Taking the vast evolutionary history of the <i>dnmt3</i> paralogs, and the localisation of their mRNAs in the ovary, we suggest there is a role for <i>dnmt3a</i> and/or <i>dnmt3x</i> in early development, and a role for DNA methylation machinery in reproduction and development of the viviparous pea aphid.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 5","pages":"534-549"},"PeriodicalIF":2.3,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12936","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141456525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial mechanism and structure–activity relationships of Bombyx mori cecropin A","authors":"Yuyuan Tian,&nbsp;Hongxian Wei,&nbsp;Fuping Lu,&nbsp;Huazhou Wu,&nbsp;Dezhao Lou,&nbsp;Shuchang Wang,&nbsp;Tao Geng","doi":"10.1111/imb.12934","DOIUrl":"10.1111/imb.12934","url":null,"abstract":"<p><i>Bombyx mori</i> cecropin A (Bmcecropin A) has antibacterial, antiviral, anti-filamentous fungal and tumour cell inhibition activities and is considered a potential succedaneum for antibiotics. We clarified the antibacterial mechanism and structure–activity relationships and then directed the structure–activity optimization of Bmcecropin A. Firstly, we found Bmcecropin A shows a strong binding force and permeability to cell membranes like a detergent; Bmcecropin A could competitively bind to the cell membrane with the cell membrane-specific dye DiI, then damaged the membrane for the access of DiI into the cytoplasm and leading to the leakage of electrolyte and proteins. Secondly, we found Bmcopropin A could also bind to and degrade DNA; furthermore, DNA library polymerase chain reaction (PCR) results indicated that Bmcecropin A inhibited DNA replication by non-specific binding. In addition, we have identified C-terminus amidation and serine-lysine- glycine (SLG) amino acids of Bmcecropin A played critical roles in the membrane damage and DNA degradation. Based on the above results, we designed a mutant of Bmcecropin A (E⁹ to H, D¹⁷ to K, K³³ to A), which showed higher antibacterial activity, thermostability and pH stability than ampicillin but no haemolytic activity. Finally, we speculated that Bmcecropin A damaged the cell membrane through a carpet model and drew the schematic diagram of its antibacterial mechanism, based on the antibacterial mechanism and the three-dimensional configuration. These findings yield insights into the mechanism of antimicrobial peptide–pathogen interaction and beneficial for the development of new antibiotics.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"708-721"},"PeriodicalIF":2.3,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome-wide response of Dermatophagoides pteronyssinus to cystatin A, a peptidase inhibitor from human skin, sheds light on its digestive physiology and allergenicity","authors":"José Cristian Vidal-Quist,&nbsp;Félix Ortego,&nbsp;Stephane Rombauts,&nbsp;Pedro Hernández-Crespo","doi":"10.1111/imb.12931","DOIUrl":"10.1111/imb.12931","url":null,"abstract":"<p>The digestive physiology of house dust mites (HDMs) is particularly relevant for their allergenicity since many of their allergens participate in digestion and are excreted into faecal pellets, a main source of exposure for allergic subjects. To gain insight into the mite dietary digestion, the genome of the HDM <i>Dermatophagoides pteronyssinus</i> was screened for genes encoding peptidases (<i>n</i> = 320), glycosylases (<i>n</i> = 77), lipases and esterases (<i>n</i> = 320), peptidase inhibitors (<i>n</i> = 65) and allergen-related proteins (<i>n</i> = 52). Basal gene expression and transcriptional responses of mites to dietary cystatin A, a cysteine endopeptidase inhibitor with previously shown antinutritional effect on mites, were analysed by RNAseq. The ingestion of cystatin A resulted in significant regulation of different cysteine endopeptidase and glycosylase genes. One Der p 1-like and two cathepsin B-like cysteine endopeptidase genes of high basal expression were induced, which suggests their prominent role in proteolytic digestion together with major allergen Der p 1. A number of genes putatively participating in the interaction of mites with their microbiota and acquired by horizontal gene transfer were repressed, including genes encoding the peptidase Der p 38, two 1,3-beta-glucanases, a lysozyme and a GH19 chitinase. Finally, the disruption of mite digestion resulted in the regulation of up to 17 allergen and isoallergen genes. Altogether, our results shed light on the putative role of specific genes in digestion and illustrate the connection between the digestive physiology of HDM and allergy.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 6","pages":"662-677"},"PeriodicalIF":2.3,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12931","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A role of epigenetic mechanisms in regulating female reproductive responses to temperature in a pest beetle","authors":"Beth A. McCaw,&nbsp;Aoife M. Leonard,&nbsp;Tyler J. Stevenson,&nbsp;Lesley T. Lancaster","doi":"10.1111/imb.12933","DOIUrl":"10.1111/imb.12933","url":null,"abstract":"<p>Many species are threatened by climate change and must rapidly respond to survive in changing environments. Epigenetic modifications, such as DNA methylation, can facilitate plastic responses by regulating gene expression in response to environmental cues. Understanding epigenetic responses is therefore essential for predicting species' ability to rapidly adapt in the context of global environmental change. Here, we investigated the functional significance of different methylation-associated cellular processes on temperature-dependent life history in seed beetles, <i>Callosobruchus maculatus</i> Fabricius 1775 (Coleoptera: Bruchidae). We assessed changes under thermal stress in (1) DNA methyltransferase (<i>Dnmt1</i> and <i>Dnmt2</i>) expression levels, (2) genome-wide methylation and (3) reproductive performance, with (2) and (3) following treatment with 3-aminobenzamide (3AB) and zebularine (Zeb) over two generations. These drugs are well-documented to alter DNA methylation across the tree of life. We found that <i>Dnmt1</i> and <i>Dnmt2</i> were expressed throughout the body in males and females, but were highly expressed in females compared with males and exhibited temperature dependence. However, whole-genome methylation did not significantly vary with temperature, and only marginally or inconclusively with drug treatment. Both 3AB and Zeb led to profound temperature-dependent shifts in female reproductive life history trade-off allocation, often increasing fitness compared with control beetles. Mismatch between magnitude of treatment effects on DNA methylation versus life history effects suggest potential of 3AB and Zeb to alter reproductive trade-offs via changes in DNA repair and recycling processes, rather than or in addition to (subtle) changes in DNA methylation. Together, our results suggest that epigenetic mechanisms relating to <i>Dnmt</i> expression, DNA repair and recycling pathways, and possibly DNA methylation, are strongly implicated in modulating insect life history trade-offs in response to temperature change.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 5","pages":"516-533"},"PeriodicalIF":2.3,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12933","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}