Biotechnology Journal最新文献_第3页

Enhanced Fecal Norovirus Detection Using Magneto-Nanocatalys–Based Immunoassay 利用基于磁性纳米催化的免疫测定法加强粪便诺罗病毒检测

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-29 DOI: 10.1002/biot.202400447

Indra Memdi Khoris, Kenta Tsuruga, Jirayu Boonyakida, Enoch Y. Park

{"title":"Enhanced Fecal Norovirus Detection Using Magneto-Nanocatalys–Based Immunoassay","authors":"Indra Memdi Khoris, Kenta Tsuruga, Jirayu Boonyakida, Enoch Y. Park","doi":"10.1002/biot.202400447","DOIUrl":"10.1002/biot.202400447","url":null,"abstract":"<div>\u0000 \u0000 A new method has been developed to improve the detection of norovirus (NoV) in complex fecal samples using nanocatalyst-based immunoassays. The method involves using multifunctional trimetallic nanoparticles, known as Ag@Fe3O4@Au NPs. These nanoparticles consist of a core of silver (Ag) and a shell of iron oxide (Fe3O4) decorated with isolated gold nanoparticles (Au NPs). The nanoparticles have enhanced catalytic activity, making them an ideal nanocatalyst for reducing 4-nitrophenol (4-NP, yellow) to 4-aminophenol (4-AP, colorless). The developed Ag@Fe3O4@Au NPs-based immunoassay achieved a limit of detection (LOD) of 1.9 pg/mL for norovirus-like particles (NoV-LP) and 6.97 RNA copy number/mL for fecal NoV. In fecal sample analysis for NoV, a heat treatment at 65°C was necessary to prevent degradation of the target protein, ensuring sensitive detection. This work successfully combined multifunctional nanocatalysts for advanced immunoassays, which could contribute to developing nano-biosensing platforms.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris 酵母死亡过程中甲醇代谢关键基因的转录下调

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-15 DOI: 10.1002/biot.202400328

Chenbo Wang, Wei Jiang, Chang Yu, Jianye Xia

{"title":"Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris","authors":"Chenbo Wang, Wei Jiang, Chang Yu, Jianye Xia","doi":"10.1002/biot.202400328","DOIUrl":"https://doi.org/10.1002/biot.202400328","url":null,"abstract":"<div>\u0000 \u0000 Pichia pastoris possesses the unique ability to utilize methanol as its sole carbon source, which makes it a proper host for producing various high-value-added products via metabolic engineering. Nevertheless, cell death has been observed during the fermentation of modified P. pastoris, with limited literature elucidating the underlying causes and mechanisms. Understanding the death mechanisms during methanol-based fermentation is crucial for optimizing fermentation strategies, enhancing the accumulation of target products, and reducing production costs. Here, we first sought to eliminate the potential causes of cell death during fermentation, such as inadequate inorganic salts and toxic by-product accumulation. The elimination of these potential causes was achieved efficiently utilizing the high-throughput fermentation equipment. Subsequently, we established a correlation between yeast cell death and the duration of the methanol metabolism period by monitoring the growth of the yeast at different fermentation stages. A critical revelation from this work came from analyzing the yeast's transcriptomic data at various stages of methanol metabolism. It was observed that a significant characteristic of yeast cell death during fermentation was the marked down-regulation of transcript levels of key enzymes involved in the methanol assimilation pathway and genes related to their biosynthesis process. The findings of this work are crucial for better understanding the causes and mechanisms of cell death for engineered P. pastoris during methanol-utilized fermentation.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142443539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermoresponsive and Supramolecular Polymers: Interesting Biomaterials for Drug Delivery 热致伸缩性和超分子聚合物：有趣的药物输送生物材料。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400379

Ahmad Darvishi, Mojtaba Ansari

引用次数: 0

A Novel and Simplified Anion Exchange Flow-Through Polishing Approach for the Separation of Full From Empty Adeno-Associated Virus Capsids 一种新颖、简化的阴离子交换流式抛光方法，用于从空腺病毒囊壳中分离完整的腺病毒囊壳。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400430

Frederik Meierrieks, Alisa Weltken, Karl Pflanz, Andreas Pickl, Benjamin Graf, Michael W. Wolff

{"title":"A Novel and Simplified Anion Exchange Flow-Through Polishing Approach for the Separation of Full From Empty Adeno-Associated Virus Capsids","authors":"Frederik Meierrieks, Alisa Weltken, Karl Pflanz, Andreas Pickl, Benjamin Graf, Michael W. Wolff","doi":"10.1002/biot.202400430","DOIUrl":"10.1002/biot.202400430","url":null,"abstract":"Adeno-associated viruses (AAV) are widely used viral vectors for in vivo gene therapy. The purification of AAV, particularly the separation of genome-containing from empty AAV capsids, is usually time-consuming and requires expensive equipment. In this study, we present a novel laboratory scale anion exchange flow-through polishing method designed to separate full and empty AAV. Once the appropriate conditions are defined, this method eliminates the need for a chromatography system. Determination of optimal polishing conditions using a chromatography system revealed that the divalent salt MgCl2 resulted in better separation of full and empty AAV than the monovalent salt NaCl. The efficacy of the method was demonstrated for three distinct AAV serotypes (AAV8, AAV5, and AAV2) on two different stationary phases: a membrane adsorber and a monolith, resulting in a 4- to 7.5-fold enrichment of full AAV particles. Moreover, the method was shown to preserve the AAV capsids’ functional potency and structural integrity. Following the successful establishment of the flow-through polishing approach, it was adapted to a manual syringe-based system. Manual flow-through polishing using the monolith or membrane adsorber achieved 3.6- and 5.4-fold enrichment of full AAV, respectively. This study demonstrates the feasibility of separating full and empty AAV without complex linear or step gradient elution and the necessity of specialized equipment. Flow-through polishing provides a rapid and easy-to-perform platform for polishing multiple vector preparations, addressing a critical aspect in the research and development of novel gene therapies.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.202400430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Glucose Sensors: Classification, Catalyst Innovation, and Sampling Mode Evolution 电化学葡萄糖传感器：分类、催化剂创新和采样模式演变。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400349

Chenyang Song, Jian Guo, Yuhan Wang, Hongying Xiang, Yufeng Yang

{"title":"Electrochemical Glucose Sensors: Classification, Catalyst Innovation, and Sampling Mode Evolution","authors":"Chenyang Song, Jian Guo, Yuhan Wang, Hongying Xiang, Yufeng Yang","doi":"10.1002/biot.202400349","DOIUrl":"10.1002/biot.202400349","url":null,"abstract":"<div>\u0000 \u0000 Glucose sensors are essential tools for monitoring blood glucose concentration in diabetic patients. In recent years, with the increasing number of individuals suffering from diabetes, blood glucose monitoring has become extremely necessary, which expedites the iteration and upgrade of glucose sensors greatly. Currently, two main types of glucose sensors are available for blood glucose testing: enzyme-based glucose sensor (EBGS) and enzyme-free glucose sensor (EFGS). For EBGS, several progresses have been made to comprehensively improve detection performance, ranging from enhancing enzyme activity, thermostability, and electron transfer properties, to introducing new materials with superior properties. For EFGS, more and more new metallic materials and their oxides are being applied to further optimize its blood glucose monitoring. Here the latest progress of electrochemical glucose sensors, their manufacturing methods, electrode materials, electrochemical parameters, and applications were summarized, the development glucose sensors with various noninvasive sampling modes were also compared.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Saccharomyces cerevisiae for Fermentative Production of Heme 发酵生产血红素的酿酒酵母代谢工程。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400351

Hyun-Jae Lee, Dong Joo Shin, Soo Bin Nho, Ki Won Lee, Sun-Ki Kim

{"title":"Metabolic Engineering of Saccharomyces cerevisiae for Fermentative Production of Heme","authors":"Hyun-Jae Lee, Dong Joo Shin, Soo Bin Nho, Ki Won Lee, Sun-Ki Kim","doi":"10.1002/biot.202400351","DOIUrl":"10.1002/biot.202400351","url":null,"abstract":"Heme is a key ingredient required to mimic the color and flavor of meat in plant-based alternatives. This study aimed to develop a yeast-based microbial cell factory for efficient and sustainable production of heme. To this end, first, Hem12p (uroporphyrinogen decarboxylase) was identified as the rate-limiting enzyme in the heme biosynthetic pathway present in Saccharomyces cerevisiae D452-2. Next, we investigated the effects of disruption of the genes involved in the competition for heme biosynthesis precursors, transcriptional repression, and heme degradation (HMX1) on heme production efficiency. Of the knock-out strains constructed in this study, only the HMX1-deficient strain produced heme at a higher concentration than the background strain without gene disruption. In addition, overexpression of PUG1 encoding a plasma membrane transporter involved in protoporphyrin IX (the precursor to heme biosynthesis) uptake led to a significant increase in intracellular heme concentration. As a result, among the various engineered strains constructed in this study, the ΔHMX1/H3&12 + PUG1 strain, the HMX1-deficient strain overexpressing HEM3, HEM12, and PUG1, produced the highest concentration of heme (4.6 mg/L) in batch fermentation, which was 3.9-fold higher than that produced by the wild-type D452-2 strain. In a glucose-limited fed-batch fermentation, the ΔHMX1/H3&12 + PUG1 strain produced 28 mg/L heme in 66 h.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.202400351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Hybrid Model Simulating Multi-Stage Continuous Fermentation of Saccharomyces cerevisiae 模拟酿酒酵母多级连续发酵的混合模型

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400232

Huidong Zhu, Jianye Xia

引用次数: 0

Development of a Human Recombinant Collagen for Vat Polymerization-Based Bioprinting 开发用于基于大桶聚合的生物打印的人类重组胶原蛋白。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400393

Domenic Schlauch, Jan Peter Ebbecke, Johanna Meyer, Tabea Marie Fleischhammer, Hamidreza Pirmahboub, Lutz Kloke, Selin Kara, Antonina Lavrentieva, Iliyana Pepelanova

{"title":"Development of a Human Recombinant Collagen for Vat Polymerization-Based Bioprinting","authors":"Domenic Schlauch, Jan Peter Ebbecke, Johanna Meyer, Tabea Marie Fleischhammer, Hamidreza Pirmahboub, Lutz Kloke, Selin Kara, Antonina Lavrentieva, Iliyana Pepelanova","doi":"10.1002/biot.202400393","DOIUrl":"10.1002/biot.202400393","url":null,"abstract":"In light-based 3D-bioprinting, gelatin methacrylate (GelMA) is one of the most widely used materials, as it supports cell attachment, and shows good biocompatibility and degradability in vivo. However, as an animal-derived material, it also causes safety concerns when used in medical applications. Gelatin is a partial hydrolysate of collagen, containing high amounts of hydroxyproline. This causes the material to form a thermally induced gel at ambient temperatures, a behavior also observed in GelMA. This temperature-dependent gelation requires precise temperature control during the bioprinting process to prevent the gelation of the material. To avoid safety concerns associated with animal-derived materials and reduce potential issues caused by thermal gelation, a recombinant human alpha-1 collagen I fragment was expressed in Komagataella phaffii without hydroxylation. The resulting protein was successfully modified with methacryloyl groups and underwent rapid photopolymerization upon ultraviolet light exposure. The developed material exhibited slightly slower polymerization and lower storage modulus compared to GelMA, while it showed higher stretchability. However, unlike the latter, the material did not undergo physical gelation at ambient temperatures, but only when cooled down to below 10°C, a characteristic that has not been described for comparable materials so far. This gelation was not caused by the formation of triple-helical structures, as shown by the absence of the characteristic peak at 220 nm in CD spectra. Moreover, the developed recombinant material facilitated cell adherence with high cell viability after crosslinking via light to a 3D structure. Furthermore, desired geometries could be easily printed on a stereolithographic bioprinter.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.202400393","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Batch Expression of an Experimental Recombinant Snakebite Antivenom Based on an Oligoclonal Mixture of Human Monoclonal Antibodies 基于人类单克隆抗体寡克隆混合物的实验性重组蛇咬伤抗蛇毒血清的单批次表达。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202400348

Anna C. Adams, Lise M. Grav, Shirin Ahmadi, Camilla Holst Dahl, Anne Ljungars, Andreas H. Laustsen, Lars K. Nielsen

{"title":"Single-Batch Expression of an Experimental Recombinant Snakebite Antivenom Based on an Oligoclonal Mixture of Human Monoclonal Antibodies","authors":"Anna C. Adams, Lise M. Grav, Shirin Ahmadi, Camilla Holst Dahl, Anne Ljungars, Andreas H. Laustsen, Lars K. Nielsen","doi":"10.1002/biot.202400348","DOIUrl":"10.1002/biot.202400348","url":null,"abstract":"Oligoclonal antibodies, which are carefully defined mixtures of monoclonal antibodies, are valuable for the treatment of complex diseases, such as infectionss and cancer. In addition to these areas of medicine, they could be utilized for the treatment of snakebite envenoming, where recombinantly produced monoclonal human antibodies could overcome many of the drawbacks accompanying traditional antivenoms. However, producing multiple individual batches of monoclonal antibodies in an industrial setting is associated with significant costs. Therefore, it is attractive to produce oligoclonal antibodies by mixing multiple antibody-producing cell lines in a single batch to have only one upstream and downstream process. In this study, we selected four antibodies that target different toxins found in the venoms of various elapid snake species, such as mambas and cobras, and generated stable antibody-producing cell lines. Upon co-cultivation, we found the cell line ratios to be stable over 7 days. The purified oligoclonal antibody cocktail contained the anticipated antibody concentrations and bound to the target toxins as expected. These results thus provide a proof of concept for the strategy of mixing multiple cell lines in a single batch to manufacture tailored antivenoms recombinantly, which could be utilized for the treatment of snakebite envenoming and in other fields where oligoclonal antibody mixtures could find utility.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.202400348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Biotechnology Journal 10/2024 发行信息：生物技术期刊 10/2024

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-10-09 DOI: 10.1002/biot.202470101

引用次数: 0