Biotechnology Journal最新文献_第2页

Multi-omics Analysis Reveals the Correlation of Gut Microbiota and Metabolites With Thalidomide Treatment for Chemotherapy-Induced Nausea and Vomiting in Small Cell Lung Cancer. 多组学分析揭示肠道微生物群和代谢物与沙利度胺治疗小细胞肺癌化疗引起的恶心和呕吐的相关性

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-04-01 DOI: 10.1002/biot.70228

Qiang-Guo Sun, Dan Zang, Yu Xin, Jia Cui, Xu Han, Jun Chen

{"title":"Multi-omics Analysis Reveals the Correlation of Gut Microbiota and Metabolites With Thalidomide Treatment for Chemotherapy-Induced Nausea and Vomiting in Small Cell Lung Cancer.","authors":"Qiang-Guo Sun, Dan Zang, Yu Xin, Jia Cui, Xu Han, Jun Chen","doi":"10.1002/biot.70228","DOIUrl":"10.1002/biot.70228","url":null,"abstract":"Small cell lung cancer (SCLC) is a highly aggressive malignancy, and chemotherapy frequently causes nausea and vomiting, which can impair treatment tolerance. Because thalidomide (THD) has shown potential clinical benefit in alleviating nausea and anorexia, we investigated whether its effects might be associated with changes in gut microbial composition and metabolite profiles. Fecal samples were collected from patients with SCLC and categorized into THD-treated and control groups. Metagenomic sequencing and nontargeted metabolomic profiling were performed to characterize microbial composition and metabolic signatures. THD treatment was also associated with higher microbial alpha diversity and increased abundance of genera such as Eubacterium and Prevotella. Metabolomic analysis identified several differential metabolites, including hydrogenated MDI, becocalcidiol, β-octylglucoside, and azelaic acid. Collectively, these findings suggest that the gut microbiota-metabolite axis may be associated with the potential effects of THD on CINV and anorexia in patients with SCLC. The identified microbial taxa and metabolites may serve as candidate biomarkers or potential therapeutic targets, although further validation in larger studies is necessary.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 4","pages":"e70228"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147696956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capacitance Technology Enables Automated Feeding, Improved Expansion, and Higher Throughput of CAR-T Cell Stirred-Tank Bioreactor Cultures. 电容技术实现了CAR-T细胞搅拌池生物反应器培养的自动进料、改进的扩张和更高的吞吐量。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-04-01 DOI: 10.1002/biot.70226

Ivano Luigi Colao, Jacob Cunningham, Matthew Lee, Stephen Goldrick, Qasim Ali Rafiq

{"title":"Capacitance Technology Enables Automated Feeding, Improved Expansion, and Higher Throughput of CAR-T Cell Stirred-Tank Bioreactor Cultures.","authors":"Ivano Luigi Colao, Jacob Cunningham, Matthew Lee, Stephen Goldrick, Qasim Ali Rafiq","doi":"10.1002/biot.70226","DOIUrl":"10.1002/biot.70226","url":null,"abstract":"Chimeric antigen receptor (CAR) T cells present a novel and transformative approach to treat certain haematological malignancies. However, CAR-T cell expansion methods are still under development and often rely on manual, low-control culture methods. The transition to bioreactors would allow for greater process control and scalability and is a key focus of research in the field. Despite this, there are few methods to determine culture progression without the need for manual cell sampling which risks introducing errors and heightening contamination risks. In this article, we assessed whether capacitance technology could deliver reliable, on-line cell concentrations by comparing T cell, and CAR-T cell bioprocesses with Chinese hamster ovary (CHO) cultures-widely used in biotechnology and with established capacitance usage. Finding that capacitance technology could accurately measure CAR-T cell concentrations, we then demonstrated the automation of feeding using capacitance-derived triggers which improved bioprocess performance in terms of cell concentration and throughput. We anticipate that this study will expand avenues of investigation regarding capacitance as a suitable process analytical technology (PAT) to enable monitoring and control of CAR-T cell manufacture, and potentially other cell and gene therapy products. It may also enable remote monitoring of multiple batches, harvest control, and the generation of large collections of process data for modelling, which will further progress the field.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 4","pages":"e70226"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocatalytic Bamberger Rearrangement for the Synthesis of 3-Amino-2-hydroxyacetophenone via Hydroxylaminobenzene Mutase Engineering and Multi-Enzyme System Assembly. 生物催化Bamberger重排合成3-氨基-2-羟基苯乙酮的羟胺苯突变酶工程和多酶系统组装。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-04-01 DOI: 10.1002/biot.70225

Heng Tang, Tian-Tian Guo, Jin-Xing Zhong, Ze-Yu Wu, Xiao-Yu Duan, Yu-Ze Sun, Ya-Ping Xue, Yu-Guo Zheng

{"title":"Biocatalytic Bamberger Rearrangement for the Synthesis of 3-Amino-2-hydroxyacetophenone via Hydroxylaminobenzene Mutase Engineering and Multi-Enzyme System Assembly.","authors":"Heng Tang, Tian-Tian Guo, Jin-Xing Zhong, Ze-Yu Wu, Xiao-Yu Duan, Yu-Ze Sun, Ya-Ping Xue, Yu-Guo Zheng","doi":"10.1002/biot.70225","DOIUrl":"https://doi.org/10.1002/biot.70225","url":null,"abstract":"The Bamberger rearrangement is a key route to functionalized aminophenols that are widely used in pharmaceutical synthesis. Hydroxylaminobenzene mutase (HabM) catalyzes a Bamberger-type isomerization in the biosynthesis of the Pranlukast intermediate 3-amino-2-hydroxyacetophenone (3AHAP), but its low catalytic efficiency and unknown structure have limited rational improvement. Here, we combine AI-assisted phylogenetic mining, structural elucidation, protein engineering, and metabolic coordination to enhance 3AHAP biosynthesis. Deep learning-guided screening identified the NRBh-HabMEo pair as the most effective combination, substantially outperforming previously reported systems. Using an integrated dry-wet strategy involving homology template search, spectroscopy, mutagenesis, Size-Exclusion-Chromatography analysis, and AlphaFold3-assisted modeling, we reveal that HabMEo is a Fe-dependent tetramer. Rational mutagenesis supported by molecular dynamics simulations yielded a synergistic triple mutant with improved pocket dynamics, optimized Fe-substrate positioning, and markedly enhanced catalytic efficiency. To alleviate reductive limitations, NAD kinase was introduced to strengthen NADPH cycling; however, increased upstream flux led to intermediate accumulation and by-product formation. This was overcome by implementing a RIAD/RIDD-based scaffold to spatially organize NR, HabM, GDH, and NADK, thereby promoting intermediate channeling and suppressing over-reduction. Overall, this study elucidates the structure of HabM and established a successful paradigm for optimizing complex multi-enzyme cascades for sustainable production of high-value biopharmaceutical intermediates.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 4","pages":"e70225"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Machine Learning Elucidates Clinical Potential of Epithelial-Mesenchymal Transition-Associated Long Non-Coding RNAs in Breast Cancer Progression. 多机器学习阐明了上皮-间质转化相关的长链非编码rna在乳腺癌进展中的临床潜力。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-04-01 DOI: 10.1002/biot.70229

Rui Zhu, Yi Li, Jiaxi Zhang, Xinbing Yang, Haochuan Guo, Zhiheng He, Xianwei Cui, Tingming Liang, Li Guo

{"title":"Multi-Machine Learning Elucidates Clinical Potential of Epithelial-Mesenchymal Transition-Associated Long Non-Coding RNAs in Breast Cancer Progression.","authors":"Rui Zhu, Yi Li, Jiaxi Zhang, Xinbing Yang, Haochuan Guo, Zhiheng He, Xianwei Cui, Tingming Liang, Li Guo","doi":"10.1002/biot.70229","DOIUrl":"https://doi.org/10.1002/biot.70229","url":null,"abstract":"Breast carcinoma (BRCA) involves multiple molecular markers, including epithelial-mesenchymal transition (EMT), which induce cell migration. However, the specific impact of long non-coding RNAs (lncRNAs) on EMT in BRCA remains uncertain. In this study, a prognostic model was constructed using EMT-associated lncRNAs (EALs), with utilization of integrative machine learning algorithms. The optimal model consisted of 15 EALs, with an AUC of 0.89 at 5 years, showing its potential as a plausible biomarker for BRCA. Among high-risk individuals, a significant increase in pathways linked to the preservation of equilibrium and immune defense was observed. Moreover, it was indicated that immunotherapy elicited negative responses in this group. Somatic mutations displayed higher TP53 rates in high-risk patients and increased CDH1/PIK3CA in low-risk ones. Notably, AC055854.1 and MIR205HG, important EALs in the model, probably regulate BRCA development through the lncRNA-microRNA-mRNA axis. Spatial transcriptome analysis revealed higher expression levels of EALs and high-risk related genes in ductal carcinoma in situ (DCIS), invasive mixed ductal/lobular carcinoma (IDC), and triple-negative BRCA (TNBC) than in breast metastasis (BMS) samples. And neutrophils were exclusively observed within the tumor microenvironment (TME) of BMS. All these findings emphasized EALs' value in revolutionizing clinical decision-making for personalized treatment strategies in BRCA cases.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 4","pages":"e70229"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization Strategies of Polymeric Hydrogels and Decellularized Extracellular Matrices for Diabetic Chronic Wound Healing 聚合物水凝胶和脱细胞细胞外基质用于糖尿病慢性伤口愈合的优化策略。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-03-20 DOI: 10.1002/biot.70218

Hongxi Liu, Langyu He, Xinyue Liu, Yiran Tang, Yuen Yee Cheng, Xiangqin Li, Hongfei Wang, Hongwei Gao, Kedong Song

{"title":"Optimization Strategies of Polymeric Hydrogels and Decellularized Extracellular Matrices for Diabetic Chronic Wound Healing","authors":"Hongxi Liu, Langyu He, Xinyue Liu, Yiran Tang, Yuen Yee Cheng, Xiangqin Li, Hongfei Wang, Hongwei Gao, Kedong Song","doi":"10.1002/biot.70218","DOIUrl":"10.1002/biot.70218","url":null,"abstract":"<div>\u0000 \u0000 The treatment of chronic wounds in diabetes represents a significant challenge in the biomedical field. This review summarizes the application of three categories of biomaterials in this area: natural polymeric materials (gelatin, alginates, hyaluronic acid, chitosan), synthetic polymeric materials (polyvinyl alcohol, polydopamine), and decellularized matrices (dECM). It elucidates the characteristics, biological functions targeting chronic diabetic wounds, optimization strategies, current challenges, and future research directions for each material category. Specific optimization approaches include chemical modification and active substance loading for natural polymers, composite formation and functional regulation for synthetic polymers, and the preparation and application of dECM. It also highlights current limitations such as low mechanical strength, suboptimal degradation rates, and insufficient long-term safety data. Future directions include smart responsive dressings, integration with novel therapeutic approaches, and personalized dECM design.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147483967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Multi-Omics Analysis Elucidates the Metabolic Basis of Enhanced Echinocandin B Biosynthesis and Guides Targeted Engineering in Aspergillus nidulans 综合多组学分析阐明棘白菌素B生物合成增强的代谢基础，指导细粒曲霉靶向工程。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-03-19 DOI: 10.1002/biot.70217

Xiaozhang Yang, Ai-Ping Pang, Hui-Ling Chen, Tong Kou, Yi-Hang Zhang, Lianggang Huang, Zhi-Qiang Liu, Yu-Guo Zheng

{"title":"Integrated Multi-Omics Analysis Elucidates the Metabolic Basis of Enhanced Echinocandin B Biosynthesis and Guides Targeted Engineering in Aspergillus nidulans","authors":"Xiaozhang Yang, Ai-Ping Pang, Hui-Ling Chen, Tong Kou, Yi-Hang Zhang, Lianggang Huang, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1002/biot.70217","DOIUrl":"10.1002/biot.70217","url":null,"abstract":"<div>\u0000 \u0000 Echinocandin B (ECB) serves as a crucial precursor for the antifungal drug anidulafungin, yet its production cost remains high due to low fermentation titers. We previously screened a highly efficient ECB producer Aspergillus nidulans ZJB16068, but its further metabolic engineering is hindered by unclear physiological and regulatory mechanisms responsible for high production. To address this, we conducted time-resolved transcriptomic and proteomic analyses across three fermentation phases (days 4, 7, and 10). Our results reveal that during the transition from growth to production, primary metabolism is systematically downregulated, while pathways supplying precursor amino acids (L-Thr, L-Pro, L-Orn) are upregulated in the later production phase. Notably, acetyl-CoA flux is redirected toward secondary metabolism through citrate synthase downregulation, accompanied by enhanced NADPH generation via the pentose phosphate pathway to support the heightened demand for reducing power. Unexpectedly, ECB biosynthetic genes are not induced, whereas the competing sterigmatocystin pathway acts as a major acetyl-CoA sink. Further analysis identified limited fatty acid β-oxidation as a constraint on acetyl-CoA availability. Overexpression of β-oxidation genes (FOX1, FOX2, POT1) increased ECB titers by 25.1%–33.5%. This study delineates the physiological phenotype underlying high ECB production and demonstrates how multi-omics-driven target identification guides effective metabolic engineering for strain enhancement.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of a Carbonyl Reductase Yields a Robust Biocatalyst for Industrial Synthesis of (R)-3-Quinuclidinol 羰基还原酶的合理设计为工业合成(R)-3-喹啉醇提供了一个强大的生物催化剂。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-03-19 DOI: 10.1002/biot.70214

Min Cao, Xiafeng Lu, Wen Zheng, Yan Wu, Yaping Deng, Xin Hao, Kai Liu, Feng Du, Muqing Ma, Mimi Duan, Rongtao Ji, Zhibo Luo

{"title":"Rational Design of a Carbonyl Reductase Yields a Robust Biocatalyst for Industrial Synthesis of (R)-3-Quinuclidinol","authors":"Min Cao, Xiafeng Lu, Wen Zheng, Yan Wu, Yaping Deng, Xin Hao, Kai Liu, Feng Du, Muqing Ma, Mimi Duan, Rongtao Ji, Zhibo Luo","doi":"10.1002/biot.70214","DOIUrl":"10.1002/biot.70214","url":null,"abstract":"<div>\u0000 \u0000 (R)-3-quinuclidinol is a pivotal chiral synthon for pharmaceuticals such as talsaclidine, revatropate, and solifenacin. Conventional chemical synthesis routes, however, suffer from inherent drawbacks including inefficient racemic resolution and dependence on costly chiral catalysts. In this study, a carbonyl reductase (CRs-7) with high activity was selected from among 20 candidates and subsequently engineered through a machine learning-assisted strategy integrated with molecular dynamics (MD) simulations. The optimal mutant, V167F/C171Y, displayed a 5.3-fold enhancement in catalytic activity relative to the wild-type enzyme. Structural and computational analyses revealed that the mutations remodel the architecture of the substrate-access tunnel, resulting in reduced nucleophilic attack distances (d1 and d2) and accelerated catalysis. Furthermore, the V167F/C171Y variant was applied in a 50-L bioreactor, wherein only 7.50 g/L DCW (dry cell weight) of whole-cell biocatalyst was required to completely convert 100 g/L substrate within 6 h, affording (R)-3-quinuclidinol with >99% conversion and enantiomeric excess (ee). The exceptional biocatalytic performance, coupled with high substrate tolerance and operational stability, underscores the potential of this engineered enzyme for sustainable industrial manufacturing.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microprinted Epoxysilane Arrays for Conducting Microarray-Based Bioassays 微印刷环氧硅烷阵列用于微阵列生物检测。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-03-19 DOI: 10.1002/biot.70215

Roshan Tosh Aggarwal, Abdullah Abdelrahman, Monserrat Roceli Herver Cruz, Smitkumar Patel, Huiyan Li

引用次数: 0

An Ad5-Based COVID-19 Vaccine Encoding SARS-CoV-2 Spike Glycoprotein Induces Measurable Antibody and Cytokine Responses in Mice 基于ad5编码SARS-CoV-2刺突糖蛋白的COVID-19疫苗在小鼠中诱导可测量的抗体和细胞因子反应

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-03-19 DOI: 10.1002/biot.70216

Fulya Erendor, Fatih Uzer, Salih Sanlioglu

{"title":"An Ad5-Based COVID-19 Vaccine Encoding SARS-CoV-2 Spike Glycoprotein Induces Measurable Antibody and Cytokine Responses in Mice","authors":"Fulya Erendor, Fatih Uzer, Salih Sanlioglu","doi":"10.1002/biot.70216","DOIUrl":"10.1002/biot.70216","url":null,"abstract":"The global SARS-CoV-2 pandemic has underlined the urgent need for effective vaccine platforms. Adenoviral vectors have gained attention due to their high transgene capacity, broad tissue tropism, and innate immunostimulatory properties. This study aimed to develop and evaluate a recombinant adenoviral vaccine, Ad5Spike, encoding the full-length SARS-CoV-2 Spike glycoprotein. The Ad5Spike vector was generated using Gateway Cloning Technology and produced by transient calcium phosphate-mediated transfection of 293A cells. Viral particles (VP) were purified via CsCl density gradient ultracentrifugation. Female BALB/c mice (6–8 weeks old, n = 5 per group per timepoint) were immunized intraperitoneally with 108, 1010, or 1012 viral particles. Humoral and cellular immune responses were evaluated at 30- and 90-days post-immunization using ELISA, ELISpot, and pseudovirus neutralization assays. Ad5Spike vaccination induced measurable anti-Spike IgG responses, with persistent antibody levels observed up to 90 days. Splenocyte analysis revealed elevated IFN-γ, TNF-α, and IL-2 secretion, consistent with initial humoral and cellular activation. Neutralizing antibody activity against a lentiviral pseudovirus bearing the SARS-CoV-2 Spike (Wuhan-1) was dose-dependent and highest in the 1012 group. In conclusion, this early preclinical study demonstrates that the Ad5Spike vaccine elicited detectable humoral and cellular immune responses, providing a proof-of-concept for the immunogenicity of this adenoviral-based platform.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13000666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Biosynthesis of Monoterpenoic Acid from Glycerol 微生物从甘油合成单萜烯酸的研究。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2026-03-12 DOI: 10.1002/biot.70209

Dianqi Yang, Mengyao Yuan, Zewei Lu, Xuxu Li, Hong Liang, Xiaoqiang Ma

{"title":"Microbial Biosynthesis of Monoterpenoic Acid from Glycerol","authors":"Dianqi Yang, Mengyao Yuan, Zewei Lu, Xuxu Li, Hong Liang, Xiaoqiang Ma","doi":"10.1002/biot.70209","DOIUrl":"10.1002/biot.70209","url":null,"abstract":"<div>\u0000 \u0000 Geranic acid, a high-value monoterpenoic acid with wide applications, faces supply constraints from the traditional plant extraction method. This study aims to develop an efficient microbial platform for its sustainable production. We engineered an Escherichia coli host by introducing the heterologous mevalonate pathway and dehydrogenases from Castellaniella defragrans (CdGaDH and CdGeDH) to construct a geranic acid biosynthetic route from glycerol. A critical enhancement was achieved by employing the endogenous dehydrogenase EcAdhP alongside CdGeDH in the wild-type strain. Subsequently, employing the strain based on the CdGeDH-CdGaDH group with deletions in the tnaA and pta genes yielded 1.29 g/L of geranic acid with superior isomeric purity (98.85%) while reducing undesirable byproducts, such as acetic acid and indole. In a 1-L bioreactor, geranic acid was produced under optimized conditions which was subsequently isolated and purified to obtain a pure product. Furthermore, the platform was extended to produce nerolic acid, and the purified geranic acid was further methylated into methyl geranate. This work provides a green method for the sustainable and stereoselective biosynthesis of monoterpenoic acids.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0