Biotechnology Journal最新文献_第4页

Issue Information: Biotechnology Journal 8/2026 期刊信息：Biotechnology Journal 8/2026

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-31 DOI: 10.1002/biot.70292

引用次数: 0

A Thermostable Multifunctional GH5 Endoglucanase From Bacillus sp. for Lignocellulosic Biomass Saccharification Bacillus sp.用于木质纤维素生物质糖化的耐热多功能GH5内切葡聚糖酶。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-31 DOI: 10.1002/biot.70290

Debjyoti Ghosh, Aditi Konar, Yi Qin Gao, Manas Mondal, Sushant K. Sinha, Supratim Datta, S. Venkata Mohan

{"title":"A Thermostable Multifunctional GH5 Endoglucanase From Bacillus sp. for Lignocellulosic Biomass Saccharification","authors":"Debjyoti Ghosh, Aditi Konar, Yi Qin Gao, Manas Mondal, Sushant K. Sinha, Supratim Datta, S. Venkata Mohan","doi":"10.1002/biot.70290","DOIUrl":"10.1002/biot.70290","url":null,"abstract":"<div>\u0000 \u0000 The enzymatic conversion of lignocellulosic biomass to glucose remains a bottleneck for cost‑effective biofuel production, largely due to the complexity and expense of enzymes. Here we describe BsEG2, a multifunctional GH5‑like endoglucanase cloned from Bacillus sp. strain BS and heterologously expressed. BsEG2 displays optimal activity at pH 6.0 and 55°C and efficiently hydrolyzes both amorphous and crystalline cellulose. Kinetic analysis on carboxymethyl cellulose (CMC) indicates high substrate affinity and catalytic efficiency. Unlike typical endoglucanases, BsEG2 combines processive endoglucanase activity with broad substrate specificity. It degrades long cellulose chains and oligosaccharides and also shows β‑glucosidase‑like activity on cellobiose, yielding glucose across a wide range of chain lengths. BsEG2 is highly thermostable (a half-life of ~15 days at 55°C), tolerant of high salt and ionic liquids, and notably resistant to cellobiose inhibition up to 200 mM. It remains active in seawater, suggesting potential to reduce freshwater demand in biomass processing. These properties, namely, multifunctionality, stability, inhibitor tolerance, and halotolerance, make BsEG2 a strong candidate for simplified, cost‑effective enzyme formulations for glucose production from lignocellulosic feedstocks.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148628899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benchmarking Ultra-Low Attachment and Photopatterned GelMA 3D Culture Platforms for Modeling Cancer Stemness in High-Grade Serous Ovarian Cancer 高级别浆液性卵巢癌肿瘤干细胞模型的超低附着和光模式GelMA三维培养平台的标杆研究

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-29 DOI: 10.1002/biot.70287

Deren Demirel Yavuz, Ferdi Oguz, Idil Su Canitez, Gizem Yilmaz Demir, Elif Merve Aydin, Merve Kayis, Burak Giray, Dogan Vatansever, Cagatay Taskiran, Emel Sokullu, Irem Durmaz Sahin

{"title":"Benchmarking Ultra-Low Attachment and Photopatterned GelMA 3D Culture Platforms for Modeling Cancer Stemness in High-Grade Serous Ovarian Cancer","authors":"Deren Demirel Yavuz, Ferdi Oguz, Idil Su Canitez, Gizem Yilmaz Demir, Elif Merve Aydin, Merve Kayis, Burak Giray, Dogan Vatansever, Cagatay Taskiran, Emel Sokullu, Irem Durmaz Sahin","doi":"10.1002/biot.70287","DOIUrl":"https://doi.org/10.1002/biot.70287","url":null,"abstract":"High-grade serous ovarian cancer (HGSOC) exhibits pronounced intratumoral heterogeneity and chemoresistance, demanding experimental platforms that more accurately recapitulate stemness-driven relapse. Despite increasing use of three-dimensional (3D) systems in cancer research, their comparative translational performance in ovarian cancer remains unclear. Here, we systematically benchmark two scalable 3D platforms, scaffold-free ultra-low attachment (ULA) spheroids and extracellular matrix mimetic photopatterned gelatin methacryloyl (GelMA) hydrogels, using HGSOC cell lines and patient-derived primary samples. Under matched conditions, both systems enhanced cancer stem cell (CSC) associated phenotypes relative to 2D culture, including induction of stemness markers, CSC surface populations, epithelial to mesenchymal transition signatures, and drug resistance. ULA enabled long-term spheroid maturation and robust expansion of intrinsically stem-like populations, whereas GelMA photopatterning provided spatially defined microenvironmental control that promoted rapid and uniform spheroid formation and efficiently induced stem-like traits in low-stemness models. Notably, baseline SOX2 expression partially correlated with chemoresistance, supporting the biological relevance of CSC enriched platforms. Collectively, this platform level comparison establishes complementary, biotechnology driven strategies for reproducible modeling of HGSOC stemness and therapy resistance, with implications for translational drug evaluation and precision oncology.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148616839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Expression of Chitinase CtChi70 in Komagataella phaffii for Sustainable N, N'-Diacetyl-Chitobiose Production From Shrimp Shells 重组几丁质酶CtChi70在法菲Komagataella phaffii中的表达及其在虾壳中可持续生产N， N'-二乙酰壳聚糖的研究

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-23 DOI: 10.1002/biot.70284

Qinghe Chu, Yifan Lin, Shiqing Zhang, Chengjuan Zou, Chunli Wang, Thanapop Soteyome, Zhenbo Xu, Yanrui Ye

{"title":"Recombinant Expression of Chitinase CtChi70 in Komagataella phaffii for Sustainable N, N'-Diacetyl-Chitobiose Production From Shrimp Shells","authors":"Qinghe Chu, Yifan Lin, Shiqing Zhang, Chengjuan Zou, Chunli Wang, Thanapop Soteyome, Zhenbo Xu, Yanrui Ye","doi":"10.1002/biot.70284","DOIUrl":"10.1002/biot.70284","url":null,"abstract":"<div>\u0000 \u0000 The rapid expansion of the global seafood processing industry has generated substantial quantities of shrimp shells and other chitin-rich waste. Recombinant chitinases provide a promising strategy for converting this biomass into high-value chitin oligosaccharides. In this study, we systematically optimized the secretory expression of the GH18 exochitinase CtChi70 in Komagataella phaffii (formerly Pichia pastoris) and examined its applicability for the enzymatic conversion of shrimp shell-derived chitin into N,N'-diacetyl-chitobiose ((GlcNAc)2). Through signal peptide optimization, gene copy number amplification, and HAC1 overexpression, extracellular chitinase activity in shake-flask cultures reached 181 U/L. Scale-up using high-cell-density fed-batch fermentation in a 7-L bioreactor further increased activity to 2151 U/L. A mild chitin extraction process was established using citric acid demineralization and low-concentration NaOH deproteinization, yielding a suitable substrate for enzymatic hydrolysis. The resulting chitin was successfully converted into high-purity (GlcNAc)2 within 24 h. Collectively, this work establishes an efficient expression strategy for CtChi70 production and demonstrates an integrated, environmentally sustainable approach for shrimp shell valorization.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Biology of Sclareol: From the Plant Biosynthetic Pathway to Engineered Microbial and Photosynthetic Chassis 菌核醇的合成生物学：从植物生物合成途径到工程微生物和光合底盘。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-23 DOI: 10.1002/biot.70285

Yichen Li, Lidan Ye, Hongwei Yu

{"title":"Synthetic Biology of Sclareol: From the Plant Biosynthetic Pathway to Engineered Microbial and Photosynthetic Chassis","authors":"Yichen Li, Lidan Ye, Hongwei Yu","doi":"10.1002/biot.70285","DOIUrl":"10.1002/biot.70285","url":null,"abstract":"<div>\u0000 \u0000 Sclareol is a highly valued bicyclic diterpene widely used as a precursor for the fragrance ambroxide and has also attracted interest as a bioactive natural product. Traditional production relies heavily on plant extraction, primarily from Salvia sclarea, an approach constrained by environmental variability, long cultivation cycles, and costly downstream purification. To overcome these supply bottlenecks, synthetic biology-enabled biomanufacturing has emerged as a sustainable and scalable alternative. Recent advances in synthetic biology and metabolic engineering have enabled the elucidation and reconstruction of the sclareol biosynthetic pathway in heterologous hosts. This review summarizes current knowledge of sclareol biosynthesis, highlights representative engineering strategies for its production in microbial cell factories, while briefly introducing emerging photosynthetic and plant-based platforms as complementary green production systems. Finally, we outline the current challenges and future perspectives for the industrial biomanufacturing of sclareol and other high-value terpenoids.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive Evolution Identifies MHY1-Mediated Transcriptional Reprogramming for Polyphenol Tolerance in Yarrowia lipolytica 脂性耶氏菌中mhy1介导的多酚耐受性转录重编程的适应性进化

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-23 DOI: 10.1002/biot.70281

Hongyi Zhou, Md Abuhena, Zhihui Shi, Hailin He, Hua Yu, Guokun Wang

{"title":"Adaptive Evolution Identifies MHY1-Mediated Transcriptional Reprogramming for Polyphenol Tolerance in Yarrowia lipolytica","authors":"Hongyi Zhou, Md Abuhena, Zhihui Shi, Hailin He, Hua Yu, Guokun Wang","doi":"10.1002/biot.70281","DOIUrl":"10.1002/biot.70281","url":null,"abstract":"<div>\u0000 \u0000 Polyphenols are bioactive compounds used in food, beverages, cosmetics, and medicine. Despite their expanding use, the mechanisms of polyphenol toxicity in microbial polyphenol producers and the adaptive strategies that confer tolerance, remain poorly characterized. Here, we applied adaptive laboratory evolution to evolve tolerance to three structurally distinct polyphenols, curcumin, naringenin, and resveratrol in Yarrowia lipolytica. Whole-genome resequencing of tolerant strains revealed recurrent loss-of-function mutations in the morphogenetic transcription factor MHY1 (YALI1_B28150g) and large segmental duplications on chromosome E, indicating multiple evolutionary solutions. Notably, resveratrol-evolved strains showed strong tolerance in the absence of MHY1 mutations, instead relying on chromosome-scale copy-number variation, highlighting compound-specific adaptive routes. Functional reconstruction demonstrated that MHY1 inactivation is sufficient to confer high-level tolerance, as both gene deletion (ΔMHY1) and independent point mutations (F240L, C153*) reproduce the tolerant phenotype across polyphenols. Transcriptomic analysis showed that MHY1 inactivation is associated with a distinct regulatory state characterized by reduced transcriptional representation of stress-associated pathways and relative stabilization of core metabolic functions. Together, these results identify MHY1 loss-of-function as a dominant and transferable regulatory mechanism for polyphenol tolerance, while also revealing alternative genome-level adaptations selected under severe chemical stress.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Microenvironment Engineering for Functional Periodontal Regeneration: Mechanisms, Biomaterial Strategies, and Translational Perspectives 功能牙周再生的免疫微环境工程：机制、生物材料策略和翻译观点。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-23 DOI: 10.1002/biot.70289

Ruyi Zhang, Huachang Zhang, Wenhua Zeng, Jiqing Zhao, Hao Xia, Nan Zhang, Keyi Li

{"title":"Immune Microenvironment Engineering for Functional Periodontal Regeneration: Mechanisms, Biomaterial Strategies, and Translational Perspectives","authors":"Ruyi Zhang, Huachang Zhang, Wenhua Zeng, Jiqing Zhao, Hao Xia, Nan Zhang, Keyi Li","doi":"10.1002/biot.70289","DOIUrl":"10.1002/biot.70289","url":null,"abstract":"<div>\u0000 \u0000 Periodontitis is a chronic inflammatory disease characterized by irreversible destruction of alveolar bone, periodontal ligament attachment, and supporting tooth structures. Emerging evidence suggests that periodontal tissue breakdown and regenerative failure are primarily driven by dysregulation of the local immune microenvironment rather than by direct bacterial insult. Therefore, conventional therapies focused primarily on microbial control are insufficient to restore immune homeostasis and functional regeneration of the cementum–periodontal ligament–alveolar bone complex. Effective periodontal regeneration requires coordinated infection management, immune modulation, oxidative stress clearance, and inflammatory microenvironment reprogramming to relieve the suppression of regenerative cells, particularly periodontal ligament stem cells. This review summarizes key regulatory networks of the periodontal immune microenvironment, with emphasis on macrophage polarization, neutrophil heterogeneity, and the imbalance between Th17 cells and regulatory T cells. We further discuss recent immune-engineering strategies for restoring periodontal homeostasis, including surface-modified biomaterials, ion-delivery systems, stem cell–derived extracellular vesicles, gene-editing technologies, and smart responsive therapeutic scaffolds. Advances in single-cell and spatial transcriptomics have revealed previously unrecognized functional subpopulations, such as NLRP3+ macrophages and SAA1+ fibroblasts, offering new opportunities for precision immunomodulation. Collectively, this review provides an integrated and translational framework for leveraging immune microenvironment–based interventions to achieve functional and predictable periodontal regeneration.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Activity of the Lysis Protein E From Phage ID52: Dependence on SecB for Efficient Host Cell Lysis 噬菌体ID52裂解蛋白E的功能活性：依赖SecB高效裂解宿主细胞。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-23 DOI: 10.1002/biot.70271

Lan Feng, Jing Fang, Yue Xu, Lun Luo, Bin Hong, Jufang Wang, Yi Ma

{"title":"Functional Activity of the Lysis Protein E From Phage ID52: Dependence on SecB for Efficient Host Cell Lysis","authors":"Lan Feng, Jing Fang, Yue Xu, Lun Luo, Bin Hong, Jufang Wang, Yi Ma","doi":"10.1002/biot.70271","DOIUrl":"10.1002/biot.70271","url":null,"abstract":"<div>\u0000 \u0000 Single gene encoded phage lysis proteins offer a promising strategy for bacterial ghost production, yet their host-dependent regulatory mechanisms remain poorly understood. Here, we investigated the lysis protein E from phage ID52 (ID52-E), which exhibits stronger lytic activity than φX174 E. By screening ID52-E-resistant mutants, we identified a four-base insertion in secB as the genetic alteration associated with lysis resistance in BL21, and CRISPR-Cas9-mediated secB disruption confirmed that SecB is required for ID52-E-mediated lysis. Proteomic analysis revealed altered protein expression in resistant mutants despite preserved bacterial morphology. Co-immunoprecipitation and biolayer interferometry supported an apparent interaction between SecB and ID52-E, with an apparent KD of 3.541 × 10−8 M under the tested 1:1 fitting model. Molecular docking, molecular dynamics simulations, mutagenesis, lysis assays, and binding measurements further implicated SecB Ala145 as a key interface residue. Together, these findings identify SecB as a host factor that facilitates ID52-E-mediated bacterial lysis and provide mechanistic insight for improving bacterial ghost production.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a Bicistronic Expression System for Mammalian Cell-Based Production of Biofunctional Recombinant Follicle Stimulating Hormone (FSH) of Bos Indicus Origin 构建基于哺乳动物细胞生产具有生物功能的重组促卵泡激素（FSH）的双链表达系统。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-09 DOI: 10.1002/biot.70279

Abhishek Das, Srimoyee Koner, Venkateswaran Ganesan, Dewanshu Sharma, Meenakshi Mansukhani, Amit Pal, Satarupa Dutta, Mohammad Kadivella, Sarwar Azam, Souvik Sen Sharma, Subeer S. Majumdar, Nirmalya Ganguli

{"title":"Engineering a Bicistronic Expression System for Mammalian Cell-Based Production of Biofunctional Recombinant Follicle Stimulating Hormone (FSH) of Bos Indicus Origin","authors":"Abhishek Das, Srimoyee Koner, Venkateswaran Ganesan, Dewanshu Sharma, Meenakshi Mansukhani, Amit Pal, Satarupa Dutta, Mohammad Kadivella, Sarwar Azam, Souvik Sen Sharma, Subeer S. Majumdar, Nirmalya Ganguli","doi":"10.1002/biot.70279","DOIUrl":"10.1002/biot.70279","url":null,"abstract":"<div>\u0000 \u0000 Assisted reproductive technologies have become crucial in addressing the growing global demand for livestock production. Follicle-stimulating hormone (FSH) is essential for superovulation in ART procedures. Animal pituitary-derived FSH is widely utilized for these purposes; however, it is costly and presents other limitations. Currently, there are very few commercially available recombinant bovine FSH products, primarily due to challenges in equimolar expression of the FSHα and FSHβ subunits, inefficient post-translational modifications that result in improper heterodimerization, and loss of bioactivity. This study, for the first time, reports the isolation and cloning of FSHα and FSHβ cDNA from Indian cattle (Bos indicus) into a bicistronic-mammalian vector, co-expressed using the P2A peptide. This approach facilitates simultaneous expression and proper dimerization in an in vitro system, producing bioactive recombinant FSH. Additionally, we identified a single-nucleotide-polymorphism (SNP) in the FSHβ region, resulting in a Serine-to-Glycine substitution, unique to Bos indicus. Notably, this SNP was found to be conserved across Indian cattle breeds. This study successfully demonstrates, for the first time, the efficient in vitro production of FSH, thereby overcoming the challenges associated with large-scale commercial production. This advancement will aid in the production of indigenous recombinant bovine FSH, enhancing outcomes in cattle breeding.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating Membrane Surface Properties via Prewetting With Polysorbate 20 to Improve Sterile Filtration of Nanoemulsions 用聚山梨酯20预湿调节膜表面性能以改善纳米乳的无菌过滤。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-09 DOI: 10.1002/biot.70280

Shreya Kapila, Milea M. Osmanski, Randal J. Soukup, Marissa E. Bradley, David Boyd, Andrew L. Zydney

{"title":"Modulating Membrane Surface Properties via Prewetting With Polysorbate 20 to Improve Sterile Filtration of Nanoemulsions","authors":"Shreya Kapila, Milea M. Osmanski, Randal J. Soukup, Marissa E. Bradley, David Boyd, Andrew L. Zydney","doi":"10.1002/biot.70280","DOIUrl":"10.1002/biot.70280","url":null,"abstract":"Nanoemulsions are promising vaccine adjuvants and drug delivery vehicles. However, sterile filtration of nanoemulsion formulations remains challenging because of the large size of the nanodroplets. This study investigates how prewetting the sterile filters with Polysorbate 20 (PS20) modulates the surface properties and enhances filtration performance of a range of sterile filters using a squalene-based nanoemulsion stabilized with PS20 and Span 85. FTIR and contact angle measurements were used to evaluate the change in filter surface chemistry. Prewetting with PS20 reduced the buffer permeability, but it generally increased the filtrate flux with the nanoemulsion, often by as much as a factor of two, due to the reduction in frictional interactions between the surfactant-coated nanodroplets and the pretreated membrane. PS20 also reduced the extent of pore blockage, leading to a dramatic increase in capacity with most sterile filters. For example, the capacity of the Millipore Express Plus increased by nearly 100-fold, compared to only a 2-fold increase in capacity for the dual-layer Pall Supor 0.8/0.2 µm filter, even though both filters are polyethersulfone. This work highlights the importance of membrane surface chemistry and the potential for modulating filter properties by prewetting with surfactants to improve the sterile filtration of nanoemulsion-based drug products.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70280","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0