Biotechnology Journal最新文献_第5页

Rocking-Induced Rapid Formation of Tumor Spheroids for Scalable 3D Tumor-Immune Coculture Assays 在可扩展的三维肿瘤免疫共培养试验中，岩石诱导快速形成肿瘤球体。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-09 DOI: 10.1002/biot.70283

Pranav Joshi, Manav Goud Vanga, Charishma Jonnadula, Minseong Lee, Victoria Gnenema, Ananya Jamdagneya, Sang-Hyun Lee, Bosung Ku, Moo-Yeal Lee

{"title":"Rocking-Induced Rapid Formation of Tumor Spheroids for Scalable 3D Tumor-Immune Coculture Assays","authors":"Pranav Joshi, Manav Goud Vanga, Charishma Jonnadula, Minseong Lee, Victoria Gnenema, Ananya Jamdagneya, Sang-Hyun Lee, Bosung Ku, Moo-Yeal Lee","doi":"10.1002/biot.70283","DOIUrl":"10.1002/biot.70283","url":null,"abstract":"<div>\u0000 \u0000 Tumor spheroids are widely used to model the tumor microenvironment and evaluate immune-mediated cytotoxicity, but conventional methods such as ultralow-attachment and hanging-drop plates typically require 48–72 h to generate stable spheroids, limiting scalability and rapid testing. Here, we report a simple method for generating uniform tumor spheroids within 7–8 h using continuous rocking in standard multiwell plates. Computational fluid dynamics simulations showed that rocking produced mild oscillatory hydrodynamic conditions, with average shear stress ranging from 0.4 to 0.8 dyn/cm2 and X-direction shear stress remaining near ±0.2 dyn/cm2, promoting controlled cell aggregation while maintaining spheroid integrity. Using this approach, Hep3B cells reproducibly formed spheroids 80–150 µm in diameter across multiple seeding densities, yielding thousands of spheroids per well. The spheroids were embedded in extracellular matrix and transferred to a 36PillarPlate for scalable 3D tumor-immune coculture assays. To demonstrate utility, Hep3B spheroids were cocultured with NK-92 cells in the presence or absence of the immunomodulator berberine. Berberine treatment enhanced NK-92 localization and spheroid disruption, resulting in reduced tumor viability. This approach reduces spheroid preparation time from days to hours and enables same-day integration into 3D assays, providing a practical platform for studying tumor-immune interactions and screening immunomodulatory therapeutics.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust Cell Segmentation for Size Distribution Estimation via Synthetic-Data Training 基于合成数据训练的鲁棒细胞分割尺寸分布估计。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-09 DOI: 10.1002/biot.70272

Han Bit Kim, Chaeeun Lee, Naeun Lee, Hyeongseok Han, Chanhun Park, Moo Sun Hong

{"title":"Robust Cell Segmentation for Size Distribution Estimation via Synthetic-Data Training","authors":"Han Bit Kim, Chaeeun Lee, Naeun Lee, Hyeongseok Han, Chanhun Park, Moo Sun Hong","doi":"10.1002/biot.70272","DOIUrl":"10.1002/biot.70272","url":null,"abstract":"Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible plastics, yet large-scale production remains limited by costly batch operations and the lack of online analytical tools. Monitoring cell size distribution serves as a strong surrogate for intracellular PHA content, but training robust cell segmentation models for industrial bioprocesses requires extensive manual annotation, which is highly labor-intensive and impractical for densely populated microscopy images. To address this critical bottleneck, an annotation-free cell segmentation and size estimation pipeline tailored for real time monitoring is presented. Rather than relying on architectural modifications, this framework introduces a system-level automated training strategy: individual cells are automatically extracted from diluted-sample microscopy images using edge enhancement and rule-based binarization, then augmented and composited onto heterogeneous backgrounds to emulate the texture of undiluted, dense cultures. This fully synthetic data generation eliminates the need for manual labeling while enabling robust training of instance segmentation models. When implemented using a Mask R-CNN backbone, the proposed pipeline consistently tracks flow cytometry forward-scatter (FSC) distribution trends under diverse imaging conditions and achieves higher correlation with FSC data compared to Cellpose and CellSAM, representative foundation models for cell segmentation. This annotation-free methodology provides a practical and reliable automated online monitoring solution for intelligent PHA manufacturing.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70272","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Transcriptomic Profiling Reveals Differences in Initiation of Antiviral Response in Low rAAV Producing HEK293 Suspension Cells 比较转录组学分析揭示低rAAV产生HEK293悬浮细胞抗病毒反应启动的差异

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-09 DOI: 10.1002/biot.70282

Georg Smesnik, Nikolaus Virgolini, Astrid Dürauer, Nicole Borth

{"title":"Comparative Transcriptomic Profiling Reveals Differences in Initiation of Antiviral Response in Low rAAV Producing HEK293 Suspension Cells","authors":"Georg Smesnik, Nikolaus Virgolini, Astrid Dürauer, Nicole Borth","doi":"10.1002/biot.70282","DOIUrl":"10.1002/biot.70282","url":null,"abstract":"Recombinant adeno-associated virus (rAAV) vectors have become the leading platform for in vivo gene delivery, yet their industrial-scale production remains inefficient and costly. Current manufacturing, which primarily relies on transient triple-plasmid transfection in HEK293-derived cells, is limited by low vector yields, high empty-to-full capsid ratios, and pronounced batch-to-batch variability. To contribute to the understanding of underlying host factors, we compared transcriptomic profiles of five HEK293-derived producer lines with varying rAAV yields over a 72-hour production time course. The low-producing BalCD line showed poor transfection efficiency, reduced plasmid-derived transcript levels, exclusive interferon expression and a rapid activation of interferon-stimulated genes (ISG), indicating strong antiviral restriction for vector productivity. In contrast, high-producing lines mounted a reduced inflammatory and innate immune response with limited induction of antiviral genes, supporting sustained vector production. Expression patterns of high-producing cell lines further exhibited transcriptional signatures resembling those seen for producer cells arrested in the G0/G1 cell cycle phase, which potentially favors efficient vector genome replication and capsid assembly. The observed differences in the regulation of antiviral and cell cycle–associated pathways highlight key molecular determinants of rAAV productivity and provide a foundation for targeted host engineering strategies aimed at enhancing rAAV manufacturing efficiency.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70282","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress and Prospects of Nanodelivery Systems in Metabolism-Regulated mRNA Vaccines 代谢调节mRNA疫苗纳米递送系统的研究进展与展望

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-09 DOI: 10.1002/biot.70278

Wanqing Wang, Hao Zhang, Shujuan Ren, Ruonan Li, Jie Chen, Beining Zhang, Jiaxin Wang, Cuiping Li, Xinrui Gao, Yaling Tian, Zhaowei Sun, Qiang Ma, Haibo Weng

{"title":"Progress and Prospects of Nanodelivery Systems in Metabolism-Regulated mRNA Vaccines","authors":"Wanqing Wang, Hao Zhang, Shujuan Ren, Ruonan Li, Jie Chen, Beining Zhang, Jiaxin Wang, Cuiping Li, Xinrui Gao, Yaling Tian, Zhaowei Sun, Qiang Ma, Haibo Weng","doi":"10.1002/biot.70278","DOIUrl":"10.1002/biot.70278","url":null,"abstract":"<div>\u0000 \u0000 mRNA vaccines face two major challenges in tumor immunotherapy: inefficient delivery and an immunosuppressive tumor microenvironment (TME) driven by metabolic reprogramming. This review proposes that nanodelivery systems can overcome both obstacles by protecting and targeting mRNA while simultaneously reshaping tumor metabolism and immunity. We summarize how nanocarriers act as “metabolism-immunity dual regulation centers” through three mechanisms: co-delivery of metabolic regulators, intrinsic modulation of immune cell metabolism via material properties, and TME-responsive spatiotemporal release. We further emphasize the central roles of the mTOR/STAT3/HIF-1α signaling axes in linking metabolic dysregulation to immune suppression and compare the metabolic adaptability of major nanocarrier platforms. Finally, we discuss rational design principles, validation strategies, clinical translation barriers, and future directions, including AI-assisted design and engineered live carriers. This review provides an integrated framework for developing next-generation antitumor mRNA vaccines. The proposed “metabolism-immunity dual regulation” strategy combines efficient delivery, metabolic reprogramming, and durable immune activation.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Mechanisms of Cellulase Biosynthesis in Trichoderma reesei: Regulatory Networks, Secretion Pathways, and Environmental Modulation 里氏木霉纤维素酶生物合成的分子机制：调控网络、分泌途径和环境调节。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-02 DOI: 10.1002/biot.70241

Shibin Cui, Qinghao Wei, Mohd Sadeeq, Jia Zuo, Peng Xiong

{"title":"Molecular Mechanisms of Cellulase Biosynthesis in Trichoderma reesei: Regulatory Networks, Secretion Pathways, and Environmental Modulation","authors":"Shibin Cui, Qinghao Wei, Mohd Sadeeq, Jia Zuo, Peng Xiong","doi":"10.1002/biot.70241","DOIUrl":"10.1002/biot.70241","url":null,"abstract":"<div>\u0000 \u0000 Trichoderma reesei (T. reesei), a filamentous fungus, has emerged as a pivotal organism in the realm of cellulase production, garnering significant attention from researchers due to its exceptional cellulolytic properties and the potential for genetic manipulation to enhance enzyme yields. Cellulases, a group of enzymes that catalyze the hydrolysis of cellulose into glucose, hold immense importance in various biotechnological applications, including biofuel production, waste management, and the textile industry. As a model organism, T. reesei offers a unique platform for studying the mechanisms underlying cellulase production, which encompasses genetic regulation, enzymatic pathways, and the influence of environmental factors such as substrate type, light, and metal ions. Multi-omics analyses have elucidated novel aspects of cellulase production mechanisms, and concurrent advances in genetic engineering provide new strategies for optimizing enzyme synthesis in T. reesei. Despite the progress made, several unresolved questions remain regarding the regulatory mechanisms of cellulase genes, highlighting the need for future research to explore these gaps and further enhance our understanding of cellulase production. This literature review aims to synthesize current knowledge on the mechanisms of cellulase production in T. reesei, discuss recent advancements, and outline future research opportunities that could lead to significant breakthroughs in this field.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Collection Protocol on the Yield and Purity of Mesenchymal Stem Cell-Derived Extracellular Vesicles Isolated From Serum-Free Media 收集方案对无血清培养基分离间充质干细胞来源的细胞外囊泡产量和纯度的影响。

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-02 DOI: 10.1002/biot.70264

Jolene Phelps, Sara Hassanpour Tamrin, Neil A. Duncan, Arindom Sen

{"title":"The Impact of Collection Protocol on the Yield and Purity of Mesenchymal Stem Cell-Derived Extracellular Vesicles Isolated From Serum-Free Media","authors":"Jolene Phelps, Sara Hassanpour Tamrin, Neil A. Duncan, Arindom Sen","doi":"10.1002/biot.70264","DOIUrl":"10.1002/biot.70264","url":null,"abstract":"The immunomodulatory and regenerative effects of mesenchymal stem cell (MSC) extracellular vesicles (EVs) have spurred the development of strategies to manufacture these EVs as therapeutics. Clinical-grade EV manufacturing requires defined serum-free media (SFM) to reduce heterogeneity and improve batch reproducibility, yet standard protocols for collecting EVs from SFM are lacking. A primary concern is the co-isolation of SFM-derived proteins with EVs during separation, which can interfere with the characterization of the collected EVs, and their subsequent application. In this study, we evaluated how removing proteins from SFM affects EV yield and purity, and examined how collection timing and cell confluence influence EV yield and proteomic profile. A defined SFM (PPRF-msc6) was compared to (i) an ultracentrifuged medium (PPRF-msc6 after overnight ultracentrifugation), and (ii) a starvation medium (PPRF-msc6 without albumin and fetuin). MSC growth and viability were reduced in starvation medium, but were not adversely impacted when using ultracentrifuged medium. Compared to unmodified PPRF-msc6, ultracentrifuged medium improved EV purity but lowered EV yield. MSC confluence impacted the proteomic profile of EV fractions, demonstrating the importance of determining when EVs are collected during the culture period. Defining protocols for MSC-EV collection contributes to standardization within the rapidly growing EV field, and informs the development of clinically relevant bioprocesses.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and Functional Characterization of a Novel GH46 Chitosanase for Efficient Chitooligosaccharide Synthesis 新型壳聚糖酶GH46高效合成壳寡糖的生化和功能表征

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-07-02 DOI: 10.1002/biot.70276

Mingxuan Wang, Ziyun Fang, Honglin Li, Jianguo Zhang

{"title":"Biochemical and Functional Characterization of a Novel GH46 Chitosanase for Efficient Chitooligosaccharide Synthesis","authors":"Mingxuan Wang, Ziyun Fang, Honglin Li, Jianguo Zhang","doi":"10.1002/biot.70276","DOIUrl":"10.1002/biot.70276","url":null,"abstract":"<div>\u0000 \u0000 Chitosan, a derivative of the abundant biopolymer chitin, holds significant biotechnological potential but is limited by its poor solubility. Its oligomeric forms, chitooligosaccharides (COSs), exhibit superior bioactivity and solubility, driving demand for efficient enzymatic production methods. This study reports the biochemical and functional characterization of a novel chitosanase, SlCsn46A, identified from Streptomyces lydicus M01 and classified into the glycoside hydrolase family 46 (GH46). The enzyme was successfully heterologously expressed in Escherichia coli and purified. SlCsn46A demonstrated high catalytic efficiency, with an optimal activity at 60°C and pH 6.0 and a maximum specific activity of 957.80 U·mg−1. It exhibited broad pH stability and significantly enhanced activity in the presence of Mn2+ and Tween 80. Kinetic analysis revealed a low Michaelis constant (Km = 0.61 mg·mL−1), indicating strong substrate affinity. Product analysis confirmed its endo-type action mode, specifically hydrolyzing chitosan to yield chitobiose [(GlcN)2] and chitotriose [(GlcN)3] as the predominant end products. These properties collectively establish SlCsn46A as an efficient and specific biocatalyst, demonstrating great potential for the targeted and efficient synthesis of low-degree-of-polymerization COS for applications in the food, agricultural, and biomedical industries.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LaeA Orchestrates Iron-Heme Supply and P450 Catalytic Efficiency for Enhanced Echinocandin B Biosynthesis in Aspergillus nidulans LaeA调节铁血红素供应和P450催化效率，促进棘白菌素B的生物合成

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-06-29 DOI: 10.1002/biot.70275

Xiaozhang Yang, Jie Gao, Dandan Shen, Yusi Zhang, Hongquan Chen, Min Zhou, Junping Zhou, Aiping Pang, Lianggang Huang, Zhiqiang Liu, Yuguo Zheng

{"title":"LaeA Orchestrates Iron-Heme Supply and P450 Catalytic Efficiency for Enhanced Echinocandin B Biosynthesis in Aspergillus nidulans","authors":"Xiaozhang Yang, Jie Gao, Dandan Shen, Yusi Zhang, Hongquan Chen, Min Zhou, Junping Zhou, Aiping Pang, Lianggang Huang, Zhiqiang Liu, Yuguo Zheng","doi":"10.1002/biot.70275","DOIUrl":"https://doi.org/10.1002/biot.70275","url":null,"abstract":"<div>\u0000 \u0000 Echinocandin B (ECB), a fungal non-ribosomal lipopeptide, serves as the exclusive natural precursor of the front-line antifungal anidulafungin. Despite its clinical importance, ECB production remains suboptimal due to incomplete understanding of its biosynthesis mechanism. The global regulator LaeA has been implicated in secondary metabolite production, yet its specific role in ECB biosynthesis remains unexplored. To address this, we successfully constructed laeA deletion and overexpression strains, and demonstrated that LaeA functionally couples morphological development with ECB productivity. Transcriptomic analysis revealed LaeA directly activated the sterigmatocystin cluster via pathway-specific regulator AflR, but indirectly influenced ECB through iron-heme cofactor synchronization rather than direct gene cluster activation. LaeA overexpression upregulated siderophore iron transporters and heme biosynthetic genes, with supplementation of Fe2+ or 5-ALA further boosting titers to 2487 ± 123 and 2697 ± 16 mg/L, respectively. To overcome P450s catalytic constraints, we screened out a novel cytochrome P450 reductase CPR2 as the optimal redox partner. Co-expression of CPR2 with bacterial hemoglobin VHb achieved synergistic enhancement, improving the ECB titer to 3170 ± 41 mg/L. This study provided a practical strategy for improving ECB production and offering insights into the versatile regulatory modes of global secondary metabolite regulators.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Bioengineering Technologies in Female Reproduction: Preclinical Advances, Translational Challenges, and Future Outlook 新兴生物工程技术在女性生殖：临床前进展，转化的挑战，和未来展望

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-06-29 DOI: 10.1002/biot.70274

Thu Hang Nguyen, Hung Anh Nguyen, Y-Van Tran Thi, Hoang Trung Nguyen, Amber Cao, Quang Loc Do, Thanh Tung Bui, Duc Trinh Chu, Hung Cao

{"title":"Emerging Bioengineering Technologies in Female Reproduction: Preclinical Advances, Translational Challenges, and Future Outlook","authors":"Thu Hang Nguyen, Hung Anh Nguyen, Y-Van Tran Thi, Hoang Trung Nguyen, Amber Cao, Quang Loc Do, Thanh Tung Bui, Duc Trinh Chu, Hung Cao","doi":"10.1002/biot.70274","DOIUrl":"https://doi.org/10.1002/biot.70274","url":null,"abstract":"<div>\u0000 \u0000 Female infertility, which affects millions of couples globally, has been a highly focused research field owing to the importance of reproduction in humans. Emerging bioengineering technologies, including tissue engineering, microfluidic chips, imaging techniques, personalized medicine, gene editing tools, and artificial intelligence, have the potential to revolutionize the existing assisted reproductive technology. These technologies have enabled creating artificial biomimetic systems for the culture of oocytes and embryos; changed the way they develop; and enhanced their competence evaluation in an automatic manner. However, the implementation and potential integration of these technologies have been a long-entrenched challenge due to the lack of standardized protocols and precise control over reproductive cycles. This review article summarizes recent advances in these innovative approaches, with an emphasis on tissue engineering and microfluidic technology. Their convergence is discussed as a potential pathway toward more integrated, precise, and personalized reproductive systems for next-generation assisted reproductive technology. In this context, key challenges related to ethics, standardization, cross-technology integration, and clinical translation are further discussed.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"21 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Biotechnology Journal 7/2026 期刊信息：Biotechnology Journal 7/2026

IF 3.7 3区生物学

Biotechnology Journal Pub Date : 2026-06-29 DOI: 10.1002/biot.70277

引用次数: 0