Biotechnology Journal最新文献_第5页

3D Printed Microfluidic Chromatographic Column for Fast Downstream Processing Development 用于快速下游加工开发的3D打印微流控色谱柱

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-10 DOI: 10.1002/biot.70095

Vladimir Matining, Mario Messina, Benedetta Sechi, Davide Moscatelli, Mattia Sponchioni

{"title":"3D Printed Microfluidic Chromatographic Column for Fast Downstream Processing Development","authors":"Vladimir Matining, Mario Messina, Benedetta Sechi, Davide Moscatelli, Mattia Sponchioni","doi":"10.1002/biot.70095","DOIUrl":"10.1002/biot.70095","url":null,"abstract":"3D printing is emerging as a promising fabrication technique for microfluidic devices. In this work, this technology was exploited in the development of a microfluidic chromatographic column with nominal volume of 54 µL. The microcolumn was packed with a cation exchange resin and characterized, using potassium iodide as a tracer, in terms of porosity (ε = 0.72), plate number, and asymmetry factor (0.8 < AS < 1.8 for flowrates >50 µL/min). To showcase the potential of this microdevice, it was exploited in the characterization of the chromatographic behavior of lysozyme. The measured saturation capacity (q∞= 88.14 g/Lresin at 340 cm/h) was in line with the manufacturer declaration (85–135 g/L at <500 cm/h). In addition, the effect of NaCl at different concentrations on the protein adsorption isotherm was characterized, demonstrating a Langmuir to anti-Langmuir transition at concentrations ≥300 mM. The axial dispersion coefficient was finally determined (<math>\u0000 <semantics>\u0000 <msub>\u0000 <mi>D</mi>\u0000 <mrow>\u0000 <mi>A</mi>\u0000 <mi>X</mi>\u0000 </mrow>\u0000 </msub>\u0000 <annotation>${{mathcal{D}}_{AX}}$</annotation>\u0000 </semantics></math>= 6.7 · 10−9 m2/s). In this way, the mcirofluidic column allowed to develop a comprehensive mechanistic model describing the transport of lysozyme in the chromatographic medium using only 30 µL of resin and <1 g of protein, addressing the issue of limited availability of biomolecules and streamlining the process development.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI-Driven Quality Monitoring and Control in Stem Cell Cultures: A Comprehensive Review 人工智能驱动的干细胞培养质量监测和控制：综合综述

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-10 DOI: 10.1002/biot.70100

Rohan Singh, Hamid Ebrahimi Orimi, Praveen Kumar Raju Pedabaliyarasimhuni, Corinne A. Hoesli, Moncef Chioua

{"title":"AI-Driven Quality Monitoring and Control in Stem Cell Cultures: A Comprehensive Review","authors":"Rohan Singh, Hamid Ebrahimi Orimi, Praveen Kumar Raju Pedabaliyarasimhuni, Corinne A. Hoesli, Moncef Chioua","doi":"10.1002/biot.70100","DOIUrl":"10.1002/biot.70100","url":null,"abstract":"Recent advancements in stem cell research forge them into one of the most promising sources for cell therapy applications. Quality monitoring in stem cell culture is essential for ensuring consistency, viability, and therapeutic efficacy. Traditional methods involve periodic sampling for conducting endpoint assays such as cell viability, proliferation, and differentiation using microscopy and flow cytometry, which are labor-intensive and often lack the real-time monitoring of the processes for scale-up applications. This paper explores artificial intelligence (AI)-driven approaches for real-time quality control, integrating machine vision, predictive modeling, and sensor-based monitoring. AI models analyze high-resolution imaging and multi-sensor data to dynamically track critical quality attributes (CQAs), including cell morphology, proliferation rate, differentiation potential, environmental stability (pH, oxygen, and nutrient levels), genetic integrity, and contamination risks. These models enable automated anomaly detection, differentiation tracking, and adaptive culture optimization. By leveraging real-time feedback systems and multi-omics integration, AI-driven techniques enhance scalability, reproducibility, and process automation in stem cell biomanufacturing. This review outlines current advancements, challenges, and future directions in AI-assisted quality monitoring and highlights its potential to improve fully automated, scalable production of stem cell lines for clinical translation and regulatory compliance in regenerative medicine.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosomal Integration of budAB Operons and Pathway Rewiring Enhance Acetoin Production From Starch in Vibrio diabolicus budAB操纵子的染色体整合和途径重布线促进了双歧弧菌淀粉乙酰素的产生

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-10 DOI: 10.1002/biot.70094

Yuan He, Guoli Lian, Ning Guo, Zheng-Jun Li

{"title":"Chromosomal Integration of budAB Operons and Pathway Rewiring Enhance Acetoin Production From Starch in Vibrio diabolicus","authors":"Yuan He, Guoli Lian, Ning Guo, Zheng-Jun Li","doi":"10.1002/biot.70094","DOIUrl":"10.1002/biot.70094","url":null,"abstract":"<div>\u0000 \u0000 Acetoin is a key platform chemical with diverse industrial applications. In this study, the marine bacterium Vibrio diabolicus, characterized by its rapid growth and strong ability to utilize starch, was systematically engineered for efficient conversion of starch into acetoin. A suicide plasmid-mediated homologous recombination system was first developed to investigate the roles of four endogenous amylase genes. Based on transcriptomic analysis, two strong constitutively active endogenous promoters were identified and functionally validated to enhance gene expression. To increase acetoin production, the 2,3-butanediol dehydrogenase gene and polyhydroxyalkanoate synthase gene were deleted, thereby eliminating carbon flux into competing pathways for 2,3-butanediol and poly-3-hydroxybutyrate biosynthesis. Subsequently, multiple copies of the budAB operon were integrated into the chromosome to strengthen the acetoin biosynthetic route. The final engineered strain produced 13.21 g/L of acetoin within 12 h of shake flask cultivation, reflecting a significant enhancement in production efficiency. This study presents the first successful case of metabolic engineering in V. diabolicus for direct and efficient production of acetoin from starch, highlighting its significant potential for industrial-scale bioproduction.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption and Separation of the Biomolecular Motors of FOF1-ATPase-Embedded Chromatophores Using Titanium Dioxide Microsphere 二氧化钛微球吸附分离fof1 - atp酶包埋色团的生物分子马达

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-07 DOI: 10.1002/biot.70093

Bang Lou, Hongzhou Liu, Weiyong Hong, Qingliang Yang, Hanbing Li, Gensheng Yang

{"title":"Adsorption and Separation of the Biomolecular Motors of FOF1-ATPase-Embedded Chromatophores Using Titanium Dioxide Microsphere","authors":"Bang Lou, Hongzhou Liu, Weiyong Hong, Qingliang Yang, Hanbing Li, Gensheng Yang","doi":"10.1002/biot.70093","DOIUrl":"10.1002/biot.70093","url":null,"abstract":"<div>\u0000 \u0000 The biomolecular motor FOF1-ATPase-embedded chromatophore, a biomolecular motor loaded into a lipid bilayer of chromatophores derived from biocells demonstrates significant potential for applications in various biomedical fields, such as targeted drug delivery within tumor microenvironments, biological tissue penetration, and biosensor detection. However, conventional purification strategies relying on gradient/ultracentrifugation remain hampered by prohibitive costs, technical complexity, and scalability constraints, critically limiting their biomedical translation. Here, we present a paradigm-shifting approach utilizing titanium dioxide (TiO2) microspheres for efficient chromatophore isolation via Lewis acid-base interactions. Through constructing chromatophore-TiO2 complexes, we systematically investigated adsorption mechanisms using isotherm modeling and FTIR spectroscopy, revealing that 7.11%–8.84% of interfacial interactions originated from physisorption. This novel strategy achieved 93.3% ± 3.21% separation efficiency and 90.7% ± 5.77% recovery rates—surpassing conventional centrifugation by 2.1-fold in operational efficiency while maintaining chromatophore integrity. Crucially, the preserved bio functionality of FoF1-ATPase post-separation was validated through sustained proton gradient-driven ATP (adenosine triphosphate) synthesis. Our findings establish TiO2-based adsorption as a robust alternative for biomotor purification and elucidate fundamental principles governing nanobiointerfaces between inorganic matrices and membrane-embedded molecular machines. This work provides a universal platform adaptable for diverse biofilm-encapsulated agents, bridging critical gaps between laboratory-scale development and clinical-scale production of advanced bionanodevices.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Small Non-Coding RNA Dynamics During Recombinant Adeno-Associated Virus Production 揭示重组腺相关病毒生产过程中的小非编码RNA动力学。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-06 DOI: 10.1002/biot.70092

Madina Burkhart, Katrin Langenbach, Karlheinz Holzmann, Nadine Hornung, Jamie-Ann Baiz, Kerstin Otte

{"title":"Unveiling Small Non-Coding RNA Dynamics During Recombinant Adeno-Associated Virus Production","authors":"Madina Burkhart, Katrin Langenbach, Karlheinz Holzmann, Nadine Hornung, Jamie-Ann Baiz, Kerstin Otte","doi":"10.1002/biot.70092","DOIUrl":"10.1002/biot.70092","url":null,"abstract":"Recombinant adeno-associated viruses (rAAVs) play a pivotal role in gene therapy, yet the molecular interactions underlying rAAV production in host cells remain incompletely understood. Non-coding RNAs (ncRNAs), particularly microRNAs (miRNAs) and small nucleolar RNAs (snoRNAs), are increasingly recognized as key regulators of viral and cellular processes. This study investigates the dynamic expression profiles of miRNAs and snoRNAs during rAAV plasmid transfection and vector production in HEK293F cells. A total of 142 miRNAs were differentially expressed during the peak phase of rAAV production, with 128 associated with the Gene Ontology term “viral process”, indicating broad involvement in host-virus interactions. Target gene analysis linked these miRNAs to biological pathways such as nucleocytoplasmic transport, innate immunity, apoptosis, and transcriptional regulation, highlighting potential roles of miRNAs in shaping the cellular environment during viral vector assembly. In contrast, snoRNAs exhibited more modest changes in expression, yet five were significantly differentially expressed during active production, suggesting a possible, underexplored involvement in viral replication. These findings illuminate the underexplored contributions of ncRNAs to the host response during rAAV biogenesis and provide a valuable resource for understanding how cellular regulatory networks are engaged throughout vector production.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exiguobacterium mexicanum PY14 Reduces Toxic Selenite to Elemental Selenium: Characterization and Mechanism 墨西哥出口杆菌PY14将有毒亚硒酸盐还原为元素硒：表征和机制。

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-06 DOI: 10.1002/biot.70096

Xueqin Fu, Zhiqin Zhu, Peipei Yin, Yi Yang, Yunhong Huang, Zhong-er Long, Zhiming Wu, Long Zou, Haiyan Ni

{"title":"Exiguobacterium mexicanum PY14 Reduces Toxic Selenite to Elemental Selenium: Characterization and Mechanism","authors":"Xueqin Fu, Zhiqin Zhu, Peipei Yin, Yi Yang, Yunhong Huang, Zhong-er Long, Zhiming Wu, Long Zou, Haiyan Ni","doi":"10.1002/biot.70096","DOIUrl":"10.1002/biot.70096","url":null,"abstract":"Microbial reduction of toxic Se(IV) oxyanions to biogenic Se(0) has garnered considerable attention for detoxification. This study presents a comprehensive investigation of Se(IV) reduction by environmentally versatile Exiguobacterium genus through integrated physicochemical, genomic, and transcriptomic analyses. Exiguobacterium mexicanum PY14 demonstrated remarkable efficiency, reducing ∼1 mM selenite to extracellular Se(0) within 12 h under aerobic conditions, with broad adaptability to pH (7–9), temperature (30–37°C), and salinity (up to 40 g L−1 NaCl). The produced Se(0) revealed crystalline nanoaggregates with biomolecular coatings. Genomic sequencing identified a chromosome and six plasmids enriched with genes for carbohydrate metabolism, inorganic ion transport, and mobile genetic elements. Transcriptomic profiling under Se(IV) stress unveiled a coordinated stress response: up-regulation of catabolic pathways (glycolysis and citric acid cycle) for energy and NAD(P)H production, bacterial motility, and chemotaxis, alongside down-regulation of energy-intensive biosynthetic processes. Notably, genes for glutathione biosynthesis (gsh), NAD(P)H generation (gntZ), and ROS scavenging (btuE) were significantly up-regulated, along with the evidence of increased GSH levels, implicating a GSH-dependent detoxification pathway driving Se(IV) reduction. These findings deepen mechanistic understanding of Se(IV) reduction mechanism within the understudied Exiguobacterium genus, and the strain's haloalkaliphilic trait underscores its potential for bioremediating Se(IV)-contaminated saline-alkaline environments.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Universal Electrode Based on Ferredoxin-NADP+ Oxidoreductase Enables Enzymatic Biofuel Cells With Broad Substrate Spectrum 基于铁氧化还原蛋白- nadp +氧化还原酶的通用电极使酶生物燃料电池具有广泛的底物谱

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-04 DOI: 10.1002/biot.70090

Hailing Dai, Guoqiang Chen, Jingjiu Mu, Afreen Shagufta, Lijuan Liu, Fan Wang, Sheng Dong, Xiao Men, Lei Wang, Haibo Zhang

引用次数: 0

Issue Information: Biotechnology Journal 8/2025 期刊信息：Biotechnology Journal 8/2025

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-04 DOI: 10.1002/biot.70097

引用次数: 0

Revolutionizing Caffeic Acid Production: Advanced Microbial Metabolic Engineering and Synthetic Biology Approaches 革命咖啡酸生产：先进的微生物代谢工程和合成生物学方法

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-08-04 DOI: 10.1002/biot.70091

Jintao Lu, Beining Wang, Xiqiang Liu, Jung-Kul Lee, Vipin Chandra Kalia, Chunjie Gong

{"title":"Revolutionizing Caffeic Acid Production: Advanced Microbial Metabolic Engineering and Synthetic Biology Approaches","authors":"Jintao Lu, Beining Wang, Xiqiang Liu, Jung-Kul Lee, Vipin Chandra Kalia, Chunjie Gong","doi":"10.1002/biot.70091","DOIUrl":"10.1002/biot.70091","url":null,"abstract":"<div>\u0000 \u0000 Caffeic acid, a high-value natural phenolic compound synthesized through plant metabolism, plays a critical role in producing phenylpropanoid derivatives and serves as a direct precursor to several key phenolic acids. As a food additive and medicine, caffeic acid has garnered significant attention for its potential in various applications. Recent advances in synthetic biology and metabolic engineering have enabled its biosynthesis via microbial cell factories. This review summarizes five strategies for optimizing caffeic acid production: caffeic acid biosynthetic pathway, modification of metabolic pathway, systems biology and synthetic biology, cofactor engineering, and modular co-culture. However, caffeic acid production via microbial chassis faces bottlenecks such as limited precursor availability for biosynthesis, toxicity from metabolic intermediates, inefficient cofactor utilization, and over-reliance on conventional host microorganisms. Breaking through these bottlenecks by integrating the five strategies outlined is expected to further increase caffeic acid production.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 8","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing the Effect of Volume on Hydrodynamics of Plant Cell Suspensions Using CFD Modeling 利用CFD模型表征体积对植物细胞悬浮液流体动力学的影响

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-07-28 DOI: 10.1002/biot.70086

Vidya Muthulakshmi Manickavasagam, Kameswararao Anupindi, Nirav Bhatt, Smita Srivastava

{"title":"Characterizing the Effect of Volume on Hydrodynamics of Plant Cell Suspensions Using CFD Modeling","authors":"Vidya Muthulakshmi Manickavasagam, Kameswararao Anupindi, Nirav Bhatt, Smita Srivastava","doi":"10.1002/biot.70086","DOIUrl":"10.1002/biot.70086","url":null,"abstract":"<div>\u0000 \u0000 Biomass productivities in shake flasks are often not reproduced in bioreactors for plant cell cultures due to change in hydrodynamics. Considering shake flask biomass productivity as benchmark, this study employs shake flask geometries as a model system to understand hydrodynamic changes with volume and identify suitable scale-up criteria for plant cell cultivations, with minimal cost and time, given their slow growth time, using computational fluid dynamics (CFD) and experiments. Cultivation of Viola odorata cells in increasing flask volumes (100–3000 mL) revealed no significant change in biomass productivity. CFD analysis indicated that volumetric oxygen mass transfer coefficient (kLa), increased up to 1000 mL and then decreased, due to saturation of energy dissipation rates (kL is a function of energy dissipation rates) and decreasing interfacial area. The unaffected biomass concentration, despite decreased kLa, suggests that kLa may not be a significant scale-up parameter. Instead, maintaining a constant shear environment, indicated by power per unit volume saturation at higher volumes, was proposed as a suitable scale-up parameter for V. odorata cell cultivation in bioreactors. Moreover, the decrease in velocity difference between fluid layers with increased flask volume, indicated that minimizing velocity gradients in bioreactors could help achieve shake flask biomass productivity.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 7","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0