不同动态共培养条件下流场的数值模拟与比较

IF 3.2 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-05-15 DOI:10.1002/biot.70039

Liying Li, Xinyue Liu, Huamao Sun, Hailin Ma, Yuen Yee Cheng, Xiangqin Li, Zhilin Jia, Jiaquan Zhao, Kedong Song

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引用次数: 0

摘要

生物反应器技术促进了细胞扩增的逐步自动化和生物功能合成替代品的发展。然而，通过实验手段很难完全了解其中形成的流场和力场环境。计算流体动力学（CFD）为分析和理解流体流动、材料扩散和流体剪切应力（FSS）对体外细胞和组织再生动力学的影响提供了一个强大的框架。本研究采用FLUENT软件对旋转细胞培养系统（RCCS）和旋转烧瓶（SF）的流场环境进行模拟计算，包括动压力、剪应力和速度分布。将用于三维细胞培养的两种直径的颗粒随机放置在不同的径向/轴向位置，并分析不同转速下RCCS和SF中颗粒的FSS。有望可视化生物反应器的流场分布和颗粒附近的局部流体动力学变化，为不同细胞-微载体复合物的动态培养/共培养提供积极的帮助。详细分析了FSS在随机排列的L和S颗粒上的分布，以评价和筛选这两个生物反应器的适宜运行条件。直观地了解生物反应器内的流场分布和局部水动力变化，有望为动态培养提供积极的帮助。颗粒在与流体旋转期间可周期性地接触新鲜的含氧介质。在叶片的上下区域产生了SF中的两个流体循环，在底部形成了一个相对静止的流体循环区域，中心的速度和压力都很低，不利于物质交换。对于某些类型的细胞或其他结构物，旋转生物反应器可能比旋转烧瓶更适合作为动态培养工具。

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Numerical Simulation and Comparison of Flow Field in Different Dynamic Co-Culture Conditions

Bioreactor technology facilitates the gradual automation of cell expansion and the development of biofunctional synthetic alternatives. However, it is difficult to fully understand the flow field and force field environments formed in it by experimental means. Computational fluid dynamics (CFD) offers a robust framework for analyzing and understanding the impacts of fluid flow, material diffusion, and fluid shear stress (FSS) on in vitro cell and tissue regeneration dynamics. In this study, the FLUENT software is used to simulate and calculate the flow field environment of the rotary cell culture system (RCCS) and spinner flask (SF), including dynamic pressure, shear stress, and velocity distribution. Particles of two diameters for three-dimensional cell culture were randomly arranged in different radial/axial positions, and the FSS on the particles in RCCS and SF at different rotational speeds was also analyzed. It is expected to visualize the flow field distribution of the bioreactor and local hydrodynamic changes near the particles, and provide positive assistance for the dynamic culture/co-culture of different cells-microcarriers complex.

The distribution of FSS on randomly arranged L and S particles was analyzed in detail to evaluate and screen the suitable operating conditions of these two bioreactors.
Visually understanding the flow field distribution and local hydrodynamic changes within the bioreactor is expected to provide positive assistance for dynamic culture.
The particles may periodically contact the fresh oxygenated medium during rotation with the fluid.
Two fluid circulations in SF were generated in the upper/lower area of the blade, and a relatively static fluid circulation area was formed at the bottom with low velocity and pressure in the center, which was not conducive to material exchange.
Rotary bioreactors may be more suitable than spinner flasks as a dynamic culture tool for some types of cells or other constructs.

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.