Advances in ε-Poly-Lysine Biosynthesis, Selection of High-Yielding Strains and Regulatory Mechanisms

IF 3.1 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-09-01 DOI:10.1002/biot.70111

Jing-Yang Cai, Cun-Jin Zhang, Jia-Qi Wang, Ai-Mei Liao, Ming Hui, Long Pan, Xu-Sheng Chen

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引用次数: 0

Abstract

ε-Poly-lysine (ε-PL) is a naturally occurring antimicrobial polypeptide that has been approved as a food preservative in several major global markets, including Japan, China, and the United States, where it is classified as Generally Recognized as Safe (GRAS). It exhibits efficacy against Gram-positive and select Gram-negative bacteria, indicating its broad potential for application in both industrial and medical sectors. The mature fermentation process of Streptomyces albulus has established it as the primary production strain for ε-PL. This article provides a comprehensive overview of the biosynthetic and antibacterial mechanisms of ε-PL and reviews the strategies for strain selection and breeding aimed at developing high-yield ε-PL-producing strains. Furthermore, it examines approaches to enhance ε-PL production through pathway-specific regulation and global metabolic engineering, while also identifying future research directions. This review aims to serve as a theoretical reference for future researchers focusing on high-yield strain breeding and metabolic engineering strategies to optimize ε-PL production.

Summary

The biosynthesis and antimicrobial mechanism of ε-PL are described, along with strategies for the selection of high-yielding strains.
Regulatory mechanisms within the complex biosynthetic metabolism network are revealed.
Strategies to improve the production of ε-PL through pathway-specific and global regulation are discussed.

Abstract Image

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ε-聚赖氨酸生物合成、高产菌株选择及调控机制研究进展

ε-聚赖氨酸（ε-PL）是一种天然存在的抗菌多肽，已在全球几个主要市场被批准作为食品防腐剂，包括日本、中国和美国，在那里它被归类为一般公认安全（GRAS）。它对革兰氏阳性菌和部分革兰氏阴性菌均有效，表明其在工业和医疗领域具有广泛的应用潜力。白色链霉菌的成熟发酵过程确定其为ε-PL的主要生产菌株。本文综述了ε-PL的生物合成及其抑菌机理，并对旨在开发高产ε-PL菌株的选育策略进行了综述。此外，本文还探讨了通过途径特异性调控和全球代谢工程来增强ε-PL产生的方法，并确定了未来的研究方向。本文旨在为今后研究高产菌株选育和优化ε-PL产量的代谢工程策略提供理论参考。综述了ε-PL的生物合成、抑菌机理及高产菌株的选育策略。揭示了复杂的生物合成代谢网络中的调节机制。讨论了通过途径特异性调控和全局调控来提高ε-PL产量的策略。

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.