IET nanobiotechnology最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Study on Enhancing Microbicidal Activity of Pure and Ion-Exchanged Zeolites Through Structural and Chemical Determinants","authors":"Hadi Tabesh,&nbsp;Shabnam Kharrazi,&nbsp;Mostafa Bashiri Barazandeh,&nbsp;Parastoo Ebadoulah Poursafa,&nbsp;Ali Poorkhalil","doi":"10.1049/nbt2/7012728","DOIUrl":"10.1049/nbt2/7012728","url":null,"abstract":"<p>Zeolites are crystalline aluminosilicate materials known for their unique structures and small pores, making them highly suitable for various applications, including antimicrobial uses. Their porous surfaces enable them to act as carriers for metal ions, enhancing their antibacterial potential. A recent comprehensive review of the literature assessed the antibacterial activity of both natural and synthetic zeolites, with a specific focus on their performance after being modified with metal ions. The study confirmed that while unmodified zeolites possess some inherent antibacterial properties, their effectiveness is generally limited to high concentrations. In contrast, zeolites modified with metal ions, such as silver (Ag), copper (Cu), or zinc (Zn), demonstrate significantly enhanced antimicrobial effects at much lower concentrations. Among the metal-modified zeolites, Ag-treated zeolite A (ZA) emerged as the most effective, exhibiting a remarkably low minimum inhibitory concentration (MIC) of just 16 µg/mL against various bacterial strains. This heightened activity is attributed to the controlled release of Ag ions and the high ion-exchange capacity of ZA, which allows for sustained antimicrobial action. These findings suggest that metal-exchanged zeolites, particularly those with high ion-retention capabilities, hold strong potential as long-lasting and efficient antimicrobial agents. Such materials could be valuable in medical, environmental, and industrial applications, especially where bacterial resistance is a growing concern.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/7012728","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multiomic Study of Platelet-Derived Extracellular Vesicles and Impact of Platelet Concentrate Sources","authors":"Andreu Miquel Amengual-Tugores,&nbsp;Carmen Ráez-Meseguer,&nbsp;Maria Antònia Forteza-Genestra,&nbsp;Javier Calvo,&nbsp;Antoni Gayà,&nbsp;Marta Monjo,&nbsp;Joana Maria Ramis","doi":"10.1049/nbt2/8358424","DOIUrl":"10.1049/nbt2/8358424","url":null,"abstract":"<p>Platelet-derived extracellular vesicles (pEVs) are a potent fraction of platelet concentrates, enhancing their therapeutic potential in regenerative medicine. This study evaluates pEV from three platelet sources: platelet lysate (PL), fresh platelets (fPs), and aged platelets (aPs), to determine how activation and storage conditions affect pEV characteristics, functionality, and molecular content. pEV are isolated using size exclusion chromatography (SEC) and characterized by transmission electron microscopy (TEM), western blot, and nanoparticle tracking analysis (NTA). Functional assays include wound healing, metabolic activity, and cytotoxicity. Protein and miRNA profiles are obtained through LC-MS/MS and miRNA arrays, followed by bioinformatic analysis. Findings show that PL-derived pEV exhibits the highest yield and purity, containing markers CD63 and CD9. Enhanced fibroblast migration in wound healing assays suggest a critical role for PL-pEV in hemostasis, proliferation, and remodeling phases. Multiomics analysis identifies upregulated miRNAs, particularly miR-210-3p and the miR-320 family, associated with wound healing. Differential protein analysis reveals an enrichment in immune response and wound healing pathways within PL-pEV. These results demonstrate the impact of platelet preparation methods on pEV molecular cargo and efficacy, with hsa-miR-320a, hsa-miR-320b, and hsa-miR-210-3p identified as key mediators supporting the clinical potential of PL-pEV in regenerative medicine.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/8358424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148084603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148080339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining the Impact of Sonodynamic Therapy With Ultrasound Wave in the Presence of Curcumin-Coated Silver Nanoparticles on the Apoptosis of MCF7 Breast Cancer Cells","authors":"Zeinab Hormozi-Moghaddam,&nbsp;Ali Neshasteh-Riz,&nbsp;Seyedeh Mona Taheri,&nbsp;Seyed Mohammad Amini,&nbsp;Ehsan Sedghinezhad","doi":"10.1049/nbt2/7036856","DOIUrl":"10.1049/nbt2/7036856","url":null,"abstract":"<p><b>Introduction:</b> Sonodynamic therapy (SDT) is a promising approach that combines low-intensity ultrasound (LIUS) with a sensitizing agent to induce therapeutic effects. Curcumin-coated silver nanoparticles (Cur@AgNPs) have shown potential as a sensitizer, demonstrating adverse effects on cancer cell survival. This study examined the apoptotic effects of US waves in the presence of Cur@AgNPs on MCF7 breast cancer cells.</p><p><b>Methods and Materials:</b> MCF7 cells were cultured and divided into different treatment groups. Cur@AgNPs were synthesized and characterized using various techniques, confirming their size to be approximately 29.3 ± 5.6 nm. The IC50 of Cur@AgNPs in MCF7 cells was determined to be 48.23 µg/ml through the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. LIUS radiation was applied to the cells in different modes, both with and without Cur@AgNPs. Cell viability was evaluated using the MTT assay and reactive oxygen species (ROS) production was measured. Colony formation assay and real-time PCR were conducted to evaluate cell death and changes in gene expression of Bcl-2-associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), and Caspase-3, respectively.</p><p><b>Results:</b> The findings confirmed the successful synthesis of Cur@AgNPs with a uniform size of approximately 29.3 ± 5.6 nm. In the continuous wave (CW) and pulse wave (PW) modes, 50% and 25%, cell viability was measured at 65.01% ± 1.35%, 73.75% ± 1.80%, and 80.76% ± 1.57%, respectively. Cell viability in CW with Cur@AgNPs was 16.9% ± 4%. The plating efficiency (PE) of the combined treatment group was 13.66 ± 1.24, compared to 39.33 ± 1.24 for the US.CW group and 68.66 ± 2.62 for the Cur@AgNPs group. Also, the expression of proapoptotic genes, such as Bax and Caspase-3, increased, while the expression of the antiapoptotic gene Bcl-2 decreased in MCF7 cells treated with the SDT. Flow cytometry analysis revealed increased rates of early apoptosis (21.22% ± 3.82%) and late apoptosis (36.59% ± 4.5%) in the US.CW + Cur@AgNPs.</p><p><b>Conclusion:</b> This study provides novel insights into the induction of apoptosis in MCF7 breast cancer cells through SDT in the presence of Cur@AgNPs as a sonosensitizer. These findings support the potential of SDT as an effective therapeutic approach for breast cancer treatment using nonionizing and noninvasive methods.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/7036856","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer","authors":"Lingling Jiao,&nbsp;Huilin Dong,&nbsp;Changjian Wu,&nbsp;Jing Liu,&nbsp;Chenhui Wang,&nbsp;Yi Cao,&nbsp;Fan Cao,&nbsp;Ying Zhu,&nbsp;Huaiyuan Zhu","doi":"10.1049/nbt2/6616433","DOIUrl":"10.1049/nbt2/6616433","url":null,"abstract":"<p>Saliva has already proven to be a prospective diagnostic bioresource for both early disease detection and timely intervention due to its easy accessibility, noninvasiveness, and reproducibility. However, the in-depth identification of salivary proteins needs to be further improved. Until now, only 3427 proteins are included in the human salivary proteome (HSP), which is far from the millions of proteins that make up humans. Here, we set out to quantitatively map the HSP in rapid and in-depth Orbitrap Astral mass spectrometer (MS) and coronal nanomagnetic bead–based proteomics workflow. Our study reported 5937 salivary proteins, which was about 73% more than that recorded in HSP. Moreover, we compared the differences between the young and aged salivary proteins. The predominant functions of the upregulated proteins in the young were related to motor proteins and cardiomyopathy, whereas those of the aged were primarily upregulated with oxidation reaction, as well as neurodegenerative disorders. It is the first study to carry out salivary proteomics using a fast and deep Orbitrap Astral MS and remarkably enlarged the number of proteins with HSP, furthermore, salivary proteomics was found to be characterized in the young and aged. With the rapidly advancing MS and proteomics technologies, we believe that salivary protein biomarkers will be more promising for clinical diagnosis and prognosis of human diseases in the future.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/6616433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148068257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles as an Alternative Strategy for the Rapid Detection of Mycobacterium tuberculosis Complex (MTBC): A Systematic Literature Review of In Vitro Studies","authors":"Abayeneh Girma,&nbsp;Fentaye Kassawmar,&nbsp;Yeshiwas Kassa,&nbsp;Yeshwas Asrat","doi":"10.1049/nbt2/4639233","DOIUrl":"10.1049/nbt2/4639233","url":null,"abstract":"<p>Worldwide, tuberculosis (TB) ranks as a second leading cause of death. The End TB strategy targets eliminating TB by 2030. Achieving this goal requires an early, accurate, and affordable diagnosis applicable in low- and middle-income countries; increasing the reach of point-of-care (POC) diagnostics is essential. Nanodiagnostics aims to enhance clinical diagnostic procedures with heightened sensitivity and accuracy by focusing on distinctive markers for early detection. A systematic search of research articles was conducted in four databases (PubMed, Scopus, Web of Science, and ScienceDirect) independently by two researchers. Publications retrieved in the independent search were mixed and imported into a single EndNote X8. The extraction of characteristics from the selected studies were carried out step by step by two independent researcher groups Abayeneh Girma and Fentaye Kassawmar and Yeshiwas Kassa and Yeshwas Asrat using a standardized data extraction format in Microsoft Excel 2021. Finally, the extracted data were combined and clearly presented in the table with the key information and findings. Inconsistencies between reviewers were resolved by discussion, and articles were included after consensus was reached. Totally, 2740 articles were retrieved, and 69 TB nanoparticle (NP)-based assays have fulfilled the inclusion criteria and included in this systematic review. The proposed platforms share the characteristics of accuracy, affordability, and swift time-to-result. Nanodiagnostics for TB now cover all clinical presentations of the disease, including active, drug-resistant, HIV-related, latent, and extrapulmonary TB. These advancements not only enhance the diagnostic landscape but also facilitate timely and effective treatment strategies, ultimately aiming to reduce the burden of TB worldwide. This review summarizes state-of-the-art knowledge of TB nanodiagnostics for the last 18 years. For fabrication concepts, detection strategies, and clinical performance, special consideration is given using various clinical specimens, and the suitability of TB nanodiagnostics for optimal MTB testing is evaluated. TB nanodiagnostics present a promising solution for meeting the stringent demands to end the TB epidemic by 2030.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/4639233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}