IET nanobiotechnology最新文献_第10页

Metabolomic profiling of ZrO2 nanoparticles in MC3T3-E1 cells ZrO2纳米颗粒在MC3T3-E1细胞中的代谢组学分析

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-28 DOI: 10.1049/nbt2.12067

Mingfu Ye, Linhu Wang, Zhang Wu, Wenjun Liu

{"title":"Metabolomic profiling of ZrO2 nanoparticles in MC3T3-E1 cells","authors":"Mingfu Ye, Linhu Wang, Zhang Wu, Wenjun Liu","doi":"10.1049/nbt2.12067","DOIUrl":"10.1049/nbt2.12067","url":null,"abstract":"The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3-E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time-of-flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3-E1 cells in response to ZrO2 NPs. The results demonstrated that MC3T3-E1 cells treated with ZrO2 NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO2 NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO2 NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO2 NPs in MC3T3-E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO2 NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO2 NP dental materials.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c9/21/NBT2-15-687.PMC8806115.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39555471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic nanobiosorbent (MG-Chi/Fe₃ O₄ ) for dispersive solid-phase extraction of Cu(II), Pb(II), and Cd(II) followed by flame atomic absorption spectrometry determination. 磁性纳米生物吸附剂MG-Chi/Fe3 O4分散固相萃取Cu(II)、Pb(II)和Cd(II)，火焰原子吸收光谱法测定。

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-02-19 DOI: 10.1049/nbt2.12025

Narges Salehi, Ali Moghimi, Hamidreza Shahbazi

{"title":"Magnetic nanobiosorbent (MG-Chi/Fe3 O4 ) for dispersive solid-phase extraction of Cu(II), Pb(II), and Cd(II) followed by flame atomic absorption spectrometry determination.","authors":"Narges Salehi, Ali Moghimi, Hamidreza Shahbazi","doi":"10.1049/nbt2.12025","DOIUrl":"https://doi.org/10.1049/nbt2.12025","url":null,"abstract":"Trace amounts of Cu (II), Pb (II), and Cd (II) in a wastewater sample were preconcentrated with a novel cross-linked magnetic chitosan modified with a new synthesised methionine-glutaraldehyde Schiff's base (MG-Chi/Fe3 O4 ) as a dispersive solid-phase extraction (DSPE) adsorbent. The adsorbed metal ions were then eluted with a specific volume of suitable solution and determined by flame atomic absorption spectrometry (FAAS). Various parameters affecting the extraction efficiency of the metal ions were investigated and optimised, including pH, amount of adsorbent, extraction time, type and volume rate of eluent, elution time, sample volume, and effect of interfering ions. The adsorption kinetics are more consistent with the pseudo-second order model. The results were statistically interpreted and the analytical performance of the proposed method was found to have preconcentration factors of 55, 60, and 50 μg L-1 for Cu(II), Pb(II), and Cd(II), respectively, limits of detection were 0.22, 0.24, and 0.10 μg L-1 for Cu(II), Pb(II), and Cd(II), respectively, with a relative standard deviation (1.5%-2.8 %), and the liner range was 5-1000 for Cu(II) and Pb(II) and 2.5-1000 for Cd(II). It was concluded that this method was suitable for successful simultaneous determination of Cu(II), Pb(II), and Cd(II) in industrial wastewater samples.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39569320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Nano-bio surface interactions, cellular internalisation in cancer cells and e-data portals of nanomaterials: A review. 纳米生物表面相互作用，癌细胞的细胞内化和纳米材料的电子数据门户:综述。

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-03-22 DOI: 10.1049/nbt2.12040

Ram Dhan Yadav, Abha Chaudhary

{"title":"Nano-bio surface interactions, cellular internalisation in cancer cells and e-data portals of nanomaterials: A review.","authors":"Ram Dhan Yadav, Abha Chaudhary","doi":"10.1049/nbt2.12040","DOIUrl":"https://doi.org/10.1049/nbt2.12040","url":null,"abstract":"Nanomaterials (NMs) have abundant applications in areas such as electronics, energy, environment industries, biosensors, nano devices, theranostic platforms, etc. Nanoparticles can increase the solubility and stability of drug-loaded materials, enhance their internalisation, protect them from initial destruction in the biological system, and lengthen their circulation time. The biological interaction of proteins present in the body fluid with NMs can change the activity and natural surface properties of NMs. The size and charge of NMs, properties of the coated and uncoated NMs, nature of proteins, cellular interactions direct their internalisation pathway in the cellular system. Thus, the present review emphasises the impact of coated, uncoated NMs, size and charge, nature of proteins on nano-bio surface interactions and on internalisation with specific focus on cancer cells. The increased activity of NPs may also result in toxicity on health and environment, thus emphasis should be given to assess the toxicity of NMs in the medical field. The e-data sharing portals of NMs have also been discussed in this review that will be helpful in providing the information about the chemical, physical, biological properties and toxicity of NMs.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development and immunobiological evaluation of nanoparticles containing an immunodominant epitope of herpes simplex virus. 含有单纯疱疹病毒免疫显性表位的纳米颗粒的开发和免疫生物学评价。

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-03-30 DOI: 10.1049/nbt2.12043

Gabriel M Hilario, Fernando B Sulczewski, Raquel Liszbinski, Larissa D Mello, Gustavo Hagen, Tiago Fazolo, Jayme Neto, Eliane Dallegrave, Pedro Romão, Tanira Aguirre, Luiz C Rodrigues Junior

{"title":"Development and immunobiological evaluation of nanoparticles containing an immunodominant epitope of herpes simplex virus.","authors":"Gabriel M Hilario, Fernando B Sulczewski, Raquel Liszbinski, Larissa D Mello, Gustavo Hagen, Tiago Fazolo, Jayme Neto, Eliane Dallegrave, Pedro Romão, Tanira Aguirre, Luiz C Rodrigues Junior","doi":"10.1049/nbt2.12043","DOIUrl":"10.1049/nbt2.12043","url":null,"abstract":"Herpes simplex virus (HSV) 1 and 2 are viruses that infect individuals worldwide and for which there is no cure or vaccine available. The protective response against herpes is mostly mediated by CD8 T lymphocytes that respond to the immunodominant SSIEFARL epitope. However, there are some obstacles concerning the use of free SSIEFARL for vaccine or immunotherapy. The aim of this study was to evaluate the feasibility of nanoencapsulation of SSIEFARL and its immunostimulatory properties. Nano/SSIEFARL was produced by interfacial polymerization in methylmetacrylate, and the physico-chemical properties, morphology and immunobiological parameters were evaluated. To evaluate the ex vivo capacity of Nano/SSIEFARL, we used splenocytes from HSV-1-infected mice to enhance the frequency of SSIEFARL-specific CD8 T lymphocytes. The results indicate that Nano/SSIEFARL has a spherical shape, an average diameter of 352 ± 22 nm, the PDI was 0.361 ± 0.009 and is negatively charged (-26.30 ± 35). The stability at 4°C was 28 days. Also, Nano/SSIEFARL is not toxic for cells at low concentrations in vitro and it is taken up by JAWS II dendritic cells. No histopathological changes were observed in kidneys, liver and lymph nodes of animals treated with Nano/SSIEFARL. Nan/SSIEFARL increased the production of IL-1β, TNF-α and IL-12 by the dendritic cells. Finally, Nano/SSIEFARL expanded the frequency of SSIEFARL-specific CD8+T lymphocytes at the same rate as free SSIEFARL. In conclusion all data together indicate that SSIEFARL is suitable for nanoencapsulation, and the system produced presents some immunoadjuvant properties that can be used to improve the immune response against herpes.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early and late effects of absorbable poly(vinyl alcohol) hernia mesh to tissue reconstruction. 可吸收聚乙烯醇疝补片对组织重建的早期和晚期影响。

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-02-02 DOI: 10.1049/nbt2.12015

Daniella Fehér, Andrea Ferencz, Györgyi Szabó, Krisztina Juhos, Domokos Csukás, Constantinos Voniatis, Lilla Reininger, Kristóf Molnár, Angéla Jedlovszky-Hajdú, György Wéber

{"title":"Early and late effects of absorbable poly(vinyl alcohol) hernia mesh to tissue reconstruction.","authors":"Daniella Fehér, Andrea Ferencz, Györgyi Szabó, Krisztina Juhos, Domokos Csukás, Constantinos Voniatis, Lilla Reininger, Kristóf Molnár, Angéla Jedlovszky-Hajdú, György Wéber","doi":"10.1049/nbt2.12015","DOIUrl":"https://doi.org/10.1049/nbt2.12015","url":null,"abstract":"Hernia is a defect of the abdominal wall. Treatment is principally surgical mesh implantation. Non-degradable surgical meshes produce numerous complications and side-effects such as inflammatory response, mesh migration and chronic pain. In contrast, the biodegradable, poly (vinyl alcohol) (PVA) based polymers have excellent chemical, mechanical and biological properties and after their degradation no chronic pain can be expected. The toxicology of PVA solution and fibers was investigated with Human dermal fibroblast- Adult cell line. Implantation tests were observed on long-term contact (rat) and large animal (swine) models. To measure the adhesion formation, Diamond and Vandendael score were used. Macroscopical and histological responses were graded from the samples. In vitro examination showed that PVA solution and fibers are biocompatible for the cells. According to the implantation tests, all samples were integrated into the surrounding tissue, and there was no foreign body reaction. The average number of adhesions was found on the non-absorbable suture line. The biocompatibility of the PVA nanofiber mesh was demonstrated. It has a non-adhesive, non-toxic and good quality structure which has the potential to be an alternative solution for the part of the hernia mesh.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39569322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image-based analysis. 定量图像分析评估生物源性氯化银纳米颗粒对胶质母细胞瘤细胞系的抗增殖作用。

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-03-22 DOI: 10.1049/nbt2.12038

Nathalia Müller, Mateus Eugenio, Luciana F Romão, Jorge Marcondes de Souza, Soniza V Alves-Leon, Loraine Campanati, Celso Sant'Anna

{"title":"Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image-based analysis.","authors":"Nathalia Müller, Mateus Eugenio, Luciana F Romão, Jorge Marcondes de Souza, Soniza V Alves-Leon, Loraine Campanati, Celso Sant'Anna","doi":"10.1049/nbt2.12038","DOIUrl":"https://doi.org/10.1049/nbt2.12038","url":null,"abstract":"Glioblastoma is the most life-threatening tumour of the central nervous system. Temozolomide (TMZ) is the first-choice oral drug for the treatment of glioblastoma, although it shows low efficacy. Silver nanoparticles (AgNPs) have been shown to exhibit biocidal activity in a variety of microorganisms, including some pathogenic microorganisms. Herein, the antiproliferative effect of AgCl-NPs on glioblastoma cell lines (GBM02 and GBM11) and on astrocytes was evaluated through automated quantitative image-based analysis (HCA) of the cells. The cells were treated with 0.1-5.0 μg/ml AgCl-NPs or with 9.7-48.5 μg/ml TMZ. Cells that received combined treatment were also analysed. At a maximum tested concentration of AgCl-NPs, GBM02 and GBM11, the growth decreased by 93% and 40%, respectively, following 72 h of treatment. TMZ treatment decreased the proliferation of GBM02 and GBM11 cells by 58% and 34%, respectively. Combinations of AgCl-NPs and TMZ showed intermediate antiproliferative effects; the lowest concentrations caused an inhibition similar to that obtained with TMZ, and the highest concentrations caused inhibition similar to that obtained with AgCl-NPs alone. No significant changes in astrocyte proliferation were observed. The authors' findings showed that HCA is a fast and reliable approach that can be used to evaluate the antiproliferative effect of the nanoparticles at the single-cell level and that AgCl-NPs are promising agents for glioblastoma treatment.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the size reduction of AgNPs loaded hydrogel and its effect on the anti-bacterial activity. 负载AgNPs的水凝胶减粒径及其对抗菌活性的影响分析。

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-03-22 DOI: 10.1049/nbt2.12037

Michelle Dsouza, Sakthi Swarrup Jayabalan

{"title":"Analysis of the size reduction of AgNPs loaded hydrogel and its effect on the anti-bacterial activity.","authors":"Michelle Dsouza, Sakthi Swarrup Jayabalan","doi":"10.1049/nbt2.12037","DOIUrl":"https://doi.org/10.1049/nbt2.12037","url":null,"abstract":"This article analyses the effect of the size reduced Silver (Ag) loaded hydrogel by (a) lyophilisation (S1) (b) ball milling (S2) techniques and its effect on anti-bacterial activity. The g loaded hydrogel, S1 and S2 shows an increase in swelling with an increase in pH. The swelling is more for Ag loaded hydrogel in low pH. For pH above 7, the swelling ratio of Ag loaded hydrogel and S1 are almost the same while S2 shows very less swelling. The anti-bacterial studies reveal that S1 and Ag loaded hydrogel reacted well in S. aureus (Staphylococcus aureus) but no zone formation was seen in S2 .whereas no zone was formed in S1 and S2 for E-coli (Escherichia coli). As the next step, the anti-bacterial activity of Ag loaded hydrogel with the addition of curcumin (CS1-size reduced by lyophilisation, CS2-size reduced by ball milling) and turmeric (TS1-size reduced by lyophilisation, TS2-size reduced by ball milling) were investigated. In case of E.coli, a zonal formation of 1.2 cm for TS1 and 1.1 cm for TS2 and 1 cm for CS1 and 0.2 cm for CS2 was observed. For S.aureus, 1.1 and 1 cm were seen for TS1 and CS1. TS2 and CS2 did not show any zone formation. These studies clearly show that size reduction by lyophilisation (S1, TS1 and CS1) is more efficient in all the cases when compared to the ball milling technique (S2, TS2 and CS2). Comparing TS1 with S1 and CS1, TS1 has highly efficient/effective anti-bacterial properties than S1 and CS1. Therefore, lyophilised hydrogel incorporating turmeric and silver (TS1) is an excellent choice compared to using curcumin for wound dressing applications.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39569321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Influence of seed coating with copper, iron and zinc nanoparticles on growth and yield of tomato 纳米铜、铁、锌包种对番茄生长和产量的影响

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-07-29 DOI: 10.1049/nbt2.12064

Xiaoqiang Zhao, Yu Chen, Huasheng Li, Jinying Lu

{"title":"Influence of seed coating with copper, iron and zinc nanoparticles on growth and yield of tomato","authors":"Xiaoqiang Zhao, Yu Chen, Huasheng Li, Jinying Lu","doi":"10.1049/nbt2.12064","DOIUrl":"10.1049/nbt2.12064","url":null,"abstract":"Neutral nanoparticles (NPs) of copper (Cu), iron (Fe) and zinc (Zn) are widely used in agriculture. Polymer seed coating with different metal NPs may supply important nutrients during plant growth and consequently enhances yields. In this research, three kinds of metal NPs were conducted to optimize the optimal concentration through seed coating for improving plant growth and productivity of tomato. Seeds of Venice tomato cultivars were coated by polymer-based mixture with different concentrations of Cu, Fe and Zn NPs, respectively. At harvest, seed germination, internode length, average weight of single fruit, yield and fruit shape index were measured. When compared with control, the internode length increased by 7.3% and 6.8% with low concentration of Fe NPs and Zn NPs, respectively. The average weight per fruit improved over control by 10.2% and 7.5% with low concentration of Cu NPs and Fe NPs, respectively. The yield with low concentration of Cu NPs and Fe NPs increased the yield by 10.7% and 6.5% compared with control. These results indicated that polymer seed coating with low concentration of metal NPs may promote the uptake of some nutrient and thus improve the productivity of tomato.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c6/60/NBT2-15-674.PMC8675844.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39555385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

One-pot synthesis chlorin e6 nano-precipitation for colorectal cancer treatment Ce6 NPs for colorectal cancer treatment 一锅合成氯- e6纳米沉淀物用于大肠癌治疗

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-07-21 DOI: 10.1049/nbt2.12065

Zhongxing Miao, Yujie Wang, Shengjie Li, Min Zhang, Meng Xu

引用次数: 2

Treatment of tumour tissue with radio-frequency hyperthermia (using antibody-carrying nanoparticles) 射频热疗治疗肿瘤组织(使用携带抗体的纳米颗粒)

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2021-07-09 DOI: 10.1049/nbt2.12061

Reza Didarian, Ibrahim Vargel

{"title":"Treatment of tumour tissue with radio-frequency hyperthermia (using antibody-carrying nanoparticles)","authors":"Reza Didarian, Ibrahim Vargel","doi":"10.1049/nbt2.12061","DOIUrl":"10.1049/nbt2.12061","url":null,"abstract":"Intelligent inorganic nanoparticles were designed and produced for use in imaging and annihilating tumour cells by radio-frequency (RF) hyperthermia. Nanoparticles synthesised to provide RF hyperthermia must have magnetite properties. For this purpose, magnetite nanoparticles were first synthesised by the coprecipitation method (10–15 NM). These superparamagnetic nanoparticles were then covered with gold ions without losing their magnetic properties. In this step, gold ions are reduced around the magnetite nanoparticles. Surface modification of the gold-coated magnetic nanoparticles was performed in the next step. A self-assembled monolayer was created using cysteamine (2-aminoethanethiol) molecules, which have two different end groups (SH and NH2). These molecules react with the gold surface by SH groups. The NH2 groups give a positive charge to the nanoparticles. After that, a monoclonal antibody (Monoclonal Anti-N-CAM Clone NCAM-OB11) was immobilised by the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide method. Then, the antenna RF system (144.00015 MHz) was created for RF hyperthermia. The antibody-nanoparticle binding rate and cytotoxicity tests were followed by in vitro and in vivo experiments. As the main result, antibody-bound gold-coated magnetic nanoparticles were successfully connected to tumour cells. After RF hyperthermia, the tumour size decreased owing to apoptosis and necrosis of tumour cells.","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39555381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1