Preparation and characterisation of ciprofloxacin-loaded silver nanoparticles for drug delivery

IF 3.8 4区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

IET nanobiotechnology Pub Date : 2022-03-25 DOI:10.1049/nbt2.12081

Samer Hasan Hussein-Al-Ali, Suha Mujahed Abudoleh, Qais Ibrahim Abdallah Abualassal, Zead Abudayeh, Yousef Aldalahmah, Mohd Zobir Hussein

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引用次数: 8

Abstract

Silver nanoparticles (AgNPs) have shown potential applications in drug delivery. In this study, the AgNPs was prepared from silver nitrate in the presence of alginate as a capping agent. The ciprofloxacin (Cipro) was loaded on the surface of AgNPs to produce Cipro-AgNPs nanocomposite. The characteristics of the Cipro-AgNPs nanocomposite were studied by X-ray diffraction (XRD), UV–Vis, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), Fourier-transform infra-red analysis (FT-IR) and zeta potential analyses. The XRD of AgNPs and Cipro-AgNPs nanocomposite data showed that both have a crystalline structure in nature. The FT-IR data indicate that the AgNPs have been wrapped by the alginate and loaded with the Cipro drug. The TEM image showed that the Cipro-AgNPs nanocomposites have an average size of 96 nm with a spherical shape. The SEM image for AgNPs and Cipro-AgNPs nanocomposites confirmed the needle-lumpy shape. The zeta potential for Cipro-AgNPs nanocomposites exhibited a positive charge with a value of 6.5 mV. The TGA for Cipro-AgNPs nanocomposites showed loss of 79.7% in total mass compared to 57.6% for AgNPs which is due to the Cipro loaded in the AgNPs. The release of Cipro from Cipro-AgNPs nanocomposites showed slow release properties which reached 98% release within 750 min, and followed the Hixson–Crowell kinetic model. In addition, the toxicity of AgNPs and Cipro-AgNPs nanocomposites was evaluated using normal (3T3) cell line. The present work suggests that Cipro-AgNPs are suitable for drug delivery.

Abstract Image

查看原文本刊更多论文

载环丙沙星载银纳米粒子的制备与表征。

银纳米粒子（AgNPs）在药物递送中显示出潜在的应用。在本研究中，以硝酸银为原料，在藻酸盐作为封端剂的存在下制备了AgNPs。将环丙沙星（Cipro）负载于AgNPs表面，制备了Cipro AgNPs纳米复合材料。通过X射线衍射（XRD）、紫外-可见光谱（UV-Vis）、透射电子显微镜（TEM）、热重分析（TGA）、扫描电子显微镜（SEM）、傅立叶变换红外光谱（FT-IR）和ζ电位分析对Cipro AgNPs纳米复合材料的特性进行了研究。AgNPs和Cipro AgNPs纳米复合材料的XRD数据表明，两者在性质上都具有晶体结构。FT-IR数据表明，AgNPs已被藻酸盐包裹并负载了Cipro药物。TEM图像显示，Cipro AgNPs纳米复合材料的平均尺寸为96nm，呈球形。AgNPs和Cipro AgNPs纳米复合材料的SEM图像证实了针状块状。Cipro AgNPs纳米复合材料的ζ电位显示出6.5 mV的正电荷。Cipro AgNP纳米复合材料显示出79.7%的总质量损失，而AgNP的TGA显示出57.6%的总质量损耗，这是由于AgNP中负载了Cipro。Cipro从Cipro AgNPs纳米复合材料中的释放表现出缓慢的释放特性，在750分钟内达到98%的释放，并遵循Hixson-Crowell动力学模型。此外，使用正常（3T3）细胞系评估AgNPs和Cipro AgNPs纳米复合材料的毒性。目前的工作表明Cipro AgNPs适合于药物递送。

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来源期刊

IET nanobiotechnology 工程技术-纳米科技

CiteScore

6.20

自引率

4.30%

发文量

审稿时长

1 months

期刊介绍： Electrical and electronic engineers have a long and illustrious history of contributing new theories and technologies to the biomedical sciences. This includes the cable theory for understanding the transmission of electrical signals in nerve axons and muscle fibres; dielectric techniques that advanced the understanding of cell membrane structures and membrane ion channels; electron and atomic force microscopy for investigating cells at the molecular level. Other engineering disciplines, along with contributions from the biological, chemical, materials and physical sciences, continue to provide groundbreaking contributions to this subject at the molecular and submolecular level. Our subject now extends from single molecule measurements using scanning probe techniques, through to interactions between cells and microstructures, micro- and nano-fluidics, and aspects of lab-on-chip technologies. The primary aim of IET Nanobiotechnology is to provide a vital resource for academic and industrial researchers operating in this exciting cross-disciplinary activity. We can only achieve this by publishing cutting edge research papers and expert review articles from the international engineering and scientific community. To attract such contributions we will exercise a commitment to our authors by ensuring that their manuscripts receive rapid constructive peer opinions and feedback across interdisciplinary boundaries. IET Nanobiotechnology covers all aspects of research and emerging technologies including, but not limited to: Fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale (including methods that employ electrokinetic, electrohydrodynamic, and optical trapping techniques) Micromachining and microfabrication tools and techniques applied to the top-down approach to nanobiotechnology Nanomachining and nanofabrication tools and techniques directed towards biomedical and biotechnological applications (e.g. applications of atomic force microscopy, scanning probe microscopy and related tools) Colloid chemistry applied to nanobiotechnology (e.g. cosmetics, suntan lotions, bio-active nanoparticles) Biosynthesis (also known as green synthesis) of nanoparticles; to be considered for publication, research papers in this area must be directed principally towards biomedical research and especially if they encompass in vivo models or proofs of concept. We welcome papers that are application-orientated or offer new concepts of substantial biomedical importance Techniques for probing cell physiology, cell adhesion sites and cell-cell communication Molecular self-assembly, including concepts of supramolecular chemistry, molecular recognition, and DNA nanotechnology Societal issues such as health and the environment Special issues. Call for papers: Smart Nanobiosensors for Next-generation Biomedical Applications - https://digital-library.theiet.org/files/IET_NBT_CFP_SNNBA.pdf Selected extended papers from the International conference of the 19th Asian BioCeramic Symposium - https://digital-library.theiet.org/files/IET_NBT_CFP_ABS.pdf