IET nanobiotechnology最新文献

{"title":"RETRACTION: Trace elements-based Auroshell gold@hematite nanostructure: Green synthesis and their hyperthermia therapy","authors":"IET Nanobiotechnology","doi":"10.1049/nbt2/9878750","DOIUrl":"https://doi.org/10.1049/nbt2/9878750","url":null,"abstract":"<p>RETRACTION: H. M. Alahdal, S. A. Abdullrezzaq, H. I. M. Amin, S. F. Alanazi, A. T. Jalil, M. Khatami, and M. M. Saleh, “Trace elements-based Auroshell gold@hematite nanostructure: Green synthesis and their hyperthermia therapy,” <i>IET Nanobiotechnol</i>ogy 17, no. 1 (2023): 22–31, https://doi.org/10.1049/nbt2.12107.</p><p>The above article, published online on 24 November 2022 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief; The Institution of Engineering and Technology; and John Wiley &amp; Sons Ltd.</p><p>The retraction has been agreed due to concerns raised by a third party. An investigation revealed several inconsistencies regarding the experiments described and the results presented. The experimental methods are not described in detail, and so the research described is not fully comprehensible for readers, not reproducible, and the editors consider the conclusions invalid. The authors were contacted for an explanation but have not addressed any of the concerns. They have been informed of the decision to retract and they disagree with it.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/9878750","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver Nanoparticles Seed Priming for Sustainable Enhancement of Durum Wheat Growth, Yield, and Nutrient Enrichment","authors":"Yasser Al Salama,&nbsp;Ibrahim Alghoraibi,&nbsp;Raghad Zein,&nbsp;Mohammad Alsouse","doi":"10.1049/nbt2/6152486","DOIUrl":"https://doi.org/10.1049/nbt2/6152486","url":null,"abstract":"<div>\u0000 <p>Achieving food security stands as a primary challenge confronting global societies today. This necessitates the development of effective strategies to increase crop productivity and enhance their specifications, aiming to meet the growing market demands sustainably and efficiently. This research was conducted over two agricultural seasons and emphasizes the ability of silver nanoparticles (AgNPs) to promote the growth and productivity of durum wheat (variety Sham 7) cultivated under the conservative conditions of Deir ez-Zor. The wheat seeds were soaked before planting with a colloidal suspension of AgNPs, prepared through an eco-friendly method utilizing an aqueous extract of <i>Eucalyptus camaldulensis</i> leaves. The best plant morphological indicators (plant height, chlorophyl content, number of branches, and number of spikes) were observed when colloidal AgNPs were used as a soaking solution compared with silver nitrate (AgNO₃)and deionized (DI) water as a control. The highest productivity parameters (grain yield, straw yield, and 1000-grain weight) were obtained when seeds were soaked in 40 ppm of AgNPs. Furthermore, the results revealed an increase in the nutrient content of grain (nitrogen, phosphorus, and potassium). This study offers valuable insights into the prospective use of AgNPs for significant improvement in wheat cultivation, increasing productivity, and improving crop quality. As a contribution to facing future challenges in the field of agriculture and ensuring sustainable food security.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2025 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/6152486","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal Stem Cell Membrane-Derived Composite System for Enhancing the Tumor Treatment Efficacy of Metal–Organic Framework Nanoparticles","authors":"Ying Tong,&nbsp;Meng Gao,&nbsp;Yingli Luo","doi":"10.1049/nbt2/1069307","DOIUrl":"10.1049/nbt2/1069307","url":null,"abstract":"<div>\u0000 <p>Mesenchymal stem cell (MSC) membrane-coated metal–organic frameworks (MOFs) represent an innovative approach to enhance the uptake and therapeutic efficacy of copper-based MOFs (Cu-MOFs) in tumor cells. By leveraging the natural homing abilities and biocompatibility of MSC membranes, Cu-MOFs can be effectively targeted to tumor sites, promoting increased cellular uptake. This coating not only facilitates superior internalization by cancer cells but also augments the therapeutic outcomes due to the enhanced delivery of copper ions. In vitro studies demonstrate that MSC membrane-coated Cu-MOFs (MSC-Cu-MOFs) significantly improve the cytotoxic effects on tumor cells compared to uncoated Cu-MOFs. This novel strategy presents a promising avenue for advancing the precision and effectiveness of cancer treatment modalities, showcasing potential for clinical applications in oncology.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLGA-PEG-c(RGDfK)-Kushenol E Micelles With a Therapeutic Potential for Targeting Ovarian Cancer","authors":"Xue Chen,&nbsp;Haopeng Wan,&nbsp;Lijuan Lu,&nbsp;Ran Li,&nbsp;Bo Sun,&nbsp;Juan Ren","doi":"10.1049/nbt2/7136323","DOIUrl":"https://doi.org/10.1049/nbt2/7136323","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> As a naturally derived inhibitor of autophagy, Kushenol E (KE) is a biprenylated flavonoid and is isolated from <i>Sophora flavescens</i>, which has been used for the treatment of cancer, hepatitis, and skin diseases. However, KE, as a poorly soluble drug, exhibited strong autophagy regulating activity in in vitro cancer cell lines, but no related studies have reported its antiovarian cancer property. Therefore, it is very beneficial to enhance the antineoplastic properties of KE by establishing an ovarian tumor-targeting nanoparticle system modified with tumor-homing c(RGDfK) peptides.</p>\u0000 <p><b>Materials and Methods:</b> In the current study, poly(lactic-co-glycolic acid)-poly(ethylene glycol)-modified with cyclic RGDfK peptide (PLGA-PEG-c(RGDfK))-KE micelles (PPCKM) were prepared to overcome the poor water solubility of KE to meet the requirement of tumor-active targeting. The effect of PPCKM on ovarian cancer was evaluated on SKOV-3 cells and xenograft models in BALB/c nude mice.</p>\u0000 <p><b>Results:</b> The PPCKM showed a higher drug cumulative release ratio (82.16 ± 7.69% vs. 34.96 ± 3.05%, at 1.5 h) with good morphology, particle size (93.41 ± 2.84 nm), and entrapment efficiency (89.7% ± 1.3%). The cell viability, migration, and apoptosis analysis of SKOV-3 cells demonstrated that PPCKM retained potent antitumor effects and promoted apoptosis at early and advanced stages with concentration-dependent. Based on the establishment of xenograft models in BALB/c nude mice, we discovered that PPCKM reduced tumor volume and weight, inhibited proliferating cell nuclear antigen (PCNA) and Ki67 expression, as well as promoted apoptosis by targeting the tumor site.</p>\u0000 <p><b>Conclusion:</b> The findings in this study suggest that PPCKM may serve as an effective therapeutic option for ovarian cancer.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/7136323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocodelivery of 5-Fluorouracil and Curcumin by RGD-Decorated Nanoliposomes Achieves Synergistic Chemotherapy for Breast Cancer","authors":"Reza Mahmoudi,&nbsp;Somayeh Mohammadi,&nbsp;Rouzbeh Mahmoudi,&nbsp;Mohamad Hassan Fouani,&nbsp;Maryam Tajali Ardakani,&nbsp;Amin Hadi,&nbsp;Mohsen Nikseresht,&nbsp;Mehrzad Jafari Barmak,&nbsp;Farzad Karimpour,&nbsp;Hassan Bardania","doi":"10.1049/nbt2/4959295","DOIUrl":"https://doi.org/10.1049/nbt2/4959295","url":null,"abstract":"<div>\u0000 <p>In the present study, arginine–glycine–aspartic acid peptide (RGD) surface functionalized liposomes (Lips) were formulated for the concomitant targeted delivery of two antineoplastic drugs, namely curcumin (Cur) and 5-fluorouracil (5FU) to breast cancer cells. The Lips’ measured size values where 50–100 nm by transmission electron microscopy (TEM) and 169 ± 10.2 nm by dynamic light scattering (DLS), which fall within the desired range required for drug delivery purposes. In this study, we assessed the antineoplastic effects of various liposomal formulations for the codelivery of Cur and 5FU to MCF-7 breast cancer cells. We evaluated two liposomal formulations (Lip–Cur–5FU) and (Lip–Cur–5FU–RGD). The treatment of MCF-7 cells with 32 µg/mL of Cur exhibited a significant (<i>p</i> &lt; 0.0001) drop in cell viability among the three formulations, namely Cur and 5Fu in the free form (Lip–Cur–5FU) and liposomal form (Lip–Cur–5FU–RGD); the least viability rate (9.91% ± 1.65%) corresponded to the RGD functionalized concomitantly Cur and 5Fu loaded Lips (Lip–Cur–5FU–RGD) formulation. On the other hand, liposomal Cur increased the rate of early apoptotic cell by 4.88% without altering the rate of late apoptotic cells. Furthermore, the concomitant treatment of MCF-7 cells with Cur and 5FU enhanced the overall apoptosis rate, where Cur–5FU in the RGD functionalized-liposomal form induced the highest (16.8%) apoptosis rate, while other Cur–5FU formulations, free and nonfunctionalized liposomal form, induced lower apoptosis rates (10.4% and 10.9%, respectively). Collectively our results demonstrated that the implementation of RGD-functionalized Lips for the concomitant delivery of Cur and 5FU enhanced their therapeutic efficacy against this breast cancer model.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/4959295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linum usitatissimum Delivery over Chitosan Nanobiopolymer: Enhanced Effects on Polycystic Ovary Syndrome Condition","authors":"Abolfazl Bayrami,&nbsp;Maryam Sojoudi,&nbsp;Shima Rahim Pouran,&nbsp;Aziz Habibi-Yangjeh,&nbsp;Sanaz Sojoudi","doi":"10.1049/2024/6693566","DOIUrl":"https://doi.org/10.1049/2024/6693566","url":null,"abstract":"<div>\u0000 <p>Herein, chitosan nanoparticle (CHIT) was used as a safe and biocompatible matrix to carry flaxseed (<i>Linum usitatissimum</i> L.) extract (FSE). The number of main features and bio-interface properties of CHIT-FSE were determined by SEM, DLS, FTIR, XRD, TGA, and zeta potential analyses and compared to those of chitosan lacking FSE. A GC–MS analysis was also conducted to reveal the bioactive compounds of FSE. The active anchoring of the FSE phytomolecules over chitosan nanoparticles with enhanced thermal and structural stability was correspondingly verified. Subsequently, the influence of CHIT-FSE, CHIT-TPP, and FSE supplementation was assessed on hormonal and biochemical markers of polycystic ovary syndrome (PCOS) in female rats and compared with untreated and healthy control groups. After 16 days of treatment, CHIT-FSE represented the best performance for controlling the serum levels of the studied biochemical (lipid profile and blood glucose level) and hormonal (insulin, testosterone, luteinizing, and follicle-stimulating hormone) parameters. Considering the negligible therapeutic activity of CHIT-TPP, the enhanced activity of CHIT-FSE compared to only FSE was expounded based on the potent action of chitosan nanoparticles in enhanced stabilization, bioavailability, transport, and permeability of the therapeutically important phytomolecules. As per the results of this investigation, supporting medically important biomolecules over chitosan can enhance their therapeutic effectiveness in controlling PCOS.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/2024/6693566","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141639570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomal-Naringenin Radiosensitizes Triple-Negative Breast Cancer MDA-MB-231 Cells In Vitro","authors":"Keenau Pearce,&nbsp;Samantha I. Cairncross,&nbsp;Mongi Benjeddou","doi":"10.1049/2024/3786627","DOIUrl":"https://doi.org/10.1049/2024/3786627","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Naringenin has shown great promise in the realm of cancer therapeutics, demonstrating excellent cytotoxic action toward cancer cells and the enhanced effects of radiation therapy <i>in vitro</i>. However, the medicinal value of naringenin is severely limited clinically by poor bioavailability. Thus, multiple drug-delivery strategies for overcoming this limitation have been developed, of which liposomes are considered the most suitable due to their amphiphilic, modifiable, and biocompatible characteristics. In this study, we investigated the role of naringenin and liposomal-delivered naringenin as adjuncts to radiotherapy in the MDA-MB-231 triple-negative breast cancer cell line <i>in vitro</i>. <i>Materials and Methods</i>. Liposomal-naringenin was synthesized by thin-film hydration and extrusion and was characterized by spectrophotometry, dynamic light scattering, and zeta potential. The effects of free-from naringenin and liposomal-naringenin were evaluated toward MDA-MB-231 cell viability when combined with varying doses of radiation. Additionally, cell growth patterns, morphology, and colony formation were evaluated. <i>Results</i>. The analysis demonstrated IC₅₀ values of 387.5 and 546.6 <i>µ</i>g/ml for naringenin and liposomal-naringenin, respectively. Naringenin and liposomal-naringenin significantly lowered cell viability, proliferation, and colony formation dose-dependently, as compared to radiation in isolation. <i>Conclusion</i>. The findings presented herein concur with previous accounts of the radiosensitizing potential of naringenin and further highlight the considerable biomedical application of liposomal-naringenin within the realm of radiotherapy.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/2024/3786627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biowaste Valorization of Palm Tree Phoenix dactylifera L. for Nanocellulose Production","authors":"Randa Mohammed Dhahi,&nbsp;Mohammed Majeed Mohammed,&nbsp;Haitham Mawlood Mikhlif","doi":"10.1049/2024/7867463","DOIUrl":"https://doi.org/10.1049/2024/7867463","url":null,"abstract":"<div>\u0000 <p>The desire to reduce reliance on oil resources arises from the concerns about carbon footprint and nonrenewability. Conversely, the global presence of over 100 million palm trees poses a significant challenge due to the substantial amount of biowaste generated annually. Additionally, the use of nanocellulose (NC) as a cost-effective material is steadily gaining recognition for its growing adaptability over time. The main goal of this study is to biosynthesized NC from Iraqi date palm <i>Phoenix dactylifera</i> leaves waste with low-concentration acid-alkali treatment. The date palm leaves waste yields 20 g of NC from 100 g of leaves before acid hydrolysis treatment. The chemical components of biosynthesized NC were 47.90%, 26.78%, and 24.67% for <i>α</i>-cellulose, hemicellulose, and lignin, respectively. In order to study their properties, NC from raw date palm leaves was studied by microscopic techniques such as scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, and atomic force microscope (AFM). SEM results revealed rod-like structured NC as well as combined long-fine fibrous structures rather than compacted bundles with sizes ranging between 31 and 74 nm. With EDX, all spectra exhibit the peaks of carbon and oxygen as the main elements with 63.8% and 10.44%, respectively, in their compositions, which relate to the typical composition of cellulose. The 3D image of AFM NC with a tapping mode presented a highly uniform distribution of NC with a size of ∼15 nm. The statistical roughness analysis shows that the obtained roughness average is 7.20 nm with the root–mean-square roughness value of 21.56 nm, which corresponded relatively with the micrographs of SEM. The results of this study demonstrate the promise of using date palm waste as raw material to produce NC as green nanocomposite from biodegradable nanomaterials for water purification and sustained drug delivery for biomedical applications. In this regard and because of the insufficient reports about the extraction of NC from palm tree leaves waste, the objective of this study was designed to fabricate NC biologically from fibers sourced from the waste of Iraqi date palm <i>P. dactylifera</i> leaves that left in agricultural lands or burned, which can be an ecological and health problem as a bionanocomposites in the medical and industrial field and as alternative resources of wood materials.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/2024/7867463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141246112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan–Aloe Vera Composition Loaded with Zinc Oxide Nanoparticles for Wound Healing: In Vitro and In Vivo Evaluations","authors":"Hasanain Adel Alawadi,&nbsp;Kamyab Andarzbakhsh,&nbsp;Ali Rastegari,&nbsp;Zohreh Mohammadi,&nbsp;Mehdi Aghsami,&nbsp;Fatemeh Saadatpour","doi":"10.1049/2024/6024411","DOIUrl":"10.1049/2024/6024411","url":null,"abstract":"<div>\u0000 <p>Global concerns due to the negative impacts of untreatable wounds, as well as the growing population of these patients, emphasize the critical need for advancements in the wound healing materials and techniques. Nanotechnology offers encouraging avenues for improving wound healing process. In this context, nanoparticles (NPs) and certain natural materials, including chitosan (CS) and aloe vera (AV), have demonstrated the potential to promote healing effects. The objective of this investigation is to assess the effect of novel fabricated nanocomposite gel containing CS, AV, and zinc oxide NPs (ZnO NPs) on the wound healing process. The ZnO NPs were synthesized and characterized by X-ray diffraction and electron microscopy. Then, CS/AV gel with different ratios was prepared and loaded with ZnO NPs. The obtained formulations were characterized <i>in vitro</i> based on an antimicrobial study, and the best formulations were used for the animal study to assess their wound healing effects in 21 days. The ZnO NPs were produced with an average 33 nm particle size and exhibited rod shape morphology. Prepared gels were homogenous with good spreadability, and CS/AV/ZnO NPs formulations showed higher antimicrobial effects against <i>Staphylococcus aureus</i>, <i>Escherichia coli, and Pseudomonas aeruginosa</i>. The wound healing findings showed significant wound area reduction in the CS/AV/ZnO NPs group compared to negative control at day 21. Histopathological assessment revealed the advantageous impact of this formulation across various stages of the wound healing process, including collagen deposition (CS/AV/ZnO NPs (2 : 1), 76.6 ± 3.3 compared to negative control, 46.2 ± 3.7) and epitheliogenesis (CS/AV/ZnO NPs (2 : 1), 3 ± 0.9 compared to negative control, 0.8 ± 0.8). CS/AV gel-loaded ZnO NPs showed significant effectiveness in wound healing and would be suggested as a promising formulation in the wound healing process. Further assessments are warranted to ensure the robustness of our findings.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/2024/6024411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal Tissue-Derived Exosomal miRNA-34a in Diabetic Nephropathy Induces Renal Tubular Cell Fibrosis by Promoting the Polarization of M1 Macrophages","authors":"Shuai Zheng,&nbsp;Yi Zeng,&nbsp;Liqing Chu,&nbsp;Taiyang Gong,&nbsp;Sihong Li,&nbsp;Min Yang","doi":"10.1049/2024/5702517","DOIUrl":"10.1049/2024/5702517","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Diabetic nephropathy (DN) is the leading cause of chronic kidney disease, and the activation and infiltration of phagocytes are critical steps of DN. This study aimed to explore the mechanism of exosomes in macrophages and diabetes nephropathy and the role of miRNA-34a, which might provide a new path for treating DN. <i>Materials and Methods</i>. The DN model was established, and the success of the model establishment was confirmed by detecting general indicators, HE staining, and immunohistochemistry. Electron microscopy and NanoSight Tracking Analysis (NTA) were used to see the morphology and size of exosomes. MiRNA-34a inhibitor, miRNA-34a mimics, pc-<i>PPARGC1A</i>, and controls were transfected in macrophages with or without kidney exosomal. A dual-luciferase reporter gene experiment verifies the targeting relationship between miRNA-34a and <i>PPARGC1A</i>. After exosomal culture, macrophages are co-cultured with normal renal tubular cells to detect renal tubular cell fibrosis. Q-PCR and western blot were undertaken to detect related RNA and proteins. <i>Results</i>. An animal model of diabetic nephropathy was successfully constructed. Macrophages could phagocytose exosomes. After ingesting model exosomes, M1 macrophages were activated, while M2 macrophages were weakened, indicating the model mice’s kidney exosomes caused the polarization. MiRNA-34a inhibitor increased <i>PPARGC1A</i> expression. MiRNA-34a expressed higher in diabetic nephropathy Model-Exo. MiRNA-34a negatively regulated <i>PPARGC1A</i>. <i>PPARGC1A</i> rescued macrophage polarization and renal tubular cell fibrosis. <i>Conclusion</i>. Exosomal miRNA-34a of tubular epithelial cells promoted M1 macrophage activation in diabetic nephropathy via negatively regulating <i>PPARGC1A</i> expression, which may provide a new direction for further exploration of DN treatment.</p>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2024 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/2024/5702517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140692126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}