Andreu Miquel Amengual-Tugores, Carmen Ráez-Meseguer, Maria Antònia Forteza-Genestra, Javier Calvo, Antoni Gayà, Marta Monjo, Joana Maria Ramis
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引用次数: 0
摘要
血小板来源的细胞外囊泡（pEVs）是血小板浓缩物的一个有效成分，增强了它们在再生医学中的治疗潜力。本研究评估了三种血小板来源的pEV：血小板裂解液（PL）、新鲜血小板（fPs）和老化血小板（aPs），以确定活化和储存条件如何影响pEV特性、功能和分子含量。pEV采用尺寸排除色谱（SEC）分离，并通过透射电子显微镜（TEM）， western blot和纳米颗粒跟踪分析（NTA）进行表征。功能分析包括伤口愈合、代谢活性和细胞毒性。通过LC-MS/MS和miRNA阵列获得蛋白质和miRNA谱，然后进行生物信息学分析。结果表明，pl衍生的pEV产率和纯度最高，含有CD63和CD9标记物。伤口愈合试验中增强的成纤维细胞迁移表明，PL-pEV在止血、增殖和重塑阶段起着关键作用。多组学分析确定了与伤口愈合相关的上调mirna，特别是miR-210-3p和miR-320家族。差异蛋白分析揭示了PL-pEV中免疫反应和伤口愈合途径的富集。这些结果证明了血小板制备方法对pEV分子载货量和疗效的影响，hsa-miR-320a、hsa-miR-320b和hsa-miR-210-3p被确定为支持PL-pEV在再生医学中的临床潜力的关键介质。
A Multiomic Study of Platelet-Derived Extracellular Vesicles and Impact of Platelet Concentrate Sources
Platelet-derived extracellular vesicles (pEVs) are a potent fraction of platelet concentrates, enhancing their therapeutic potential in regenerative medicine. This study evaluates pEV from three platelet sources: platelet lysate (PL), fresh platelets (fPs), and aged platelets (aPs), to determine how activation and storage conditions affect pEV characteristics, functionality, and molecular content. pEV are isolated using size exclusion chromatography (SEC) and characterized by transmission electron microscopy (TEM), western blot, and nanoparticle tracking analysis (NTA). Functional assays include wound healing, metabolic activity, and cytotoxicity. Protein and miRNA profiles are obtained through LC-MS/MS and miRNA arrays, followed by bioinformatic analysis. Findings show that PL-derived pEV exhibits the highest yield and purity, containing markers CD63 and CD9. Enhanced fibroblast migration in wound healing assays suggest a critical role for PL-pEV in hemostasis, proliferation, and remodeling phases. Multiomics analysis identifies upregulated miRNAs, particularly miR-210-3p and the miR-320 family, associated with wound healing. Differential protein analysis reveals an enrichment in immune response and wound healing pathways within PL-pEV. These results demonstrate the impact of platelet preparation methods on pEV molecular cargo and efficacy, with hsa-miR-320a, hsa-miR-320b, and hsa-miR-210-3p identified as key mediators supporting the clinical potential of PL-pEV in regenerative medicine.
期刊介绍：
Electrical and electronic engineers have a long and illustrious history of contributing new theories and technologies to the biomedical sciences. This includes the cable theory for understanding the transmission of electrical signals in nerve axons and muscle fibres; dielectric techniques that advanced the understanding of cell membrane structures and membrane ion channels; electron and atomic force microscopy for investigating cells at the molecular level.
Other engineering disciplines, along with contributions from the biological, chemical, materials and physical sciences, continue to provide groundbreaking contributions to this subject at the molecular and submolecular level. Our subject now extends from single molecule measurements using scanning probe techniques, through to interactions between cells and microstructures, micro- and nano-fluidics, and aspects of lab-on-chip technologies. The primary aim of IET Nanobiotechnology is to provide a vital resource for academic and industrial researchers operating in this exciting cross-disciplinary activity. We can only achieve this by publishing cutting edge research papers and expert review articles from the international engineering and scientific community. To attract such contributions we will exercise a commitment to our authors by ensuring that their manuscripts receive rapid constructive peer opinions and feedback across interdisciplinary boundaries.
IET Nanobiotechnology covers all aspects of research and emerging technologies including, but not limited to:
Fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale (including methods that employ electrokinetic, electrohydrodynamic, and optical trapping techniques)
Micromachining and microfabrication tools and techniques applied to the top-down approach to nanobiotechnology
Nanomachining and nanofabrication tools and techniques directed towards biomedical and biotechnological applications (e.g. applications of atomic force microscopy, scanning probe microscopy and related tools)
Colloid chemistry applied to nanobiotechnology (e.g. cosmetics, suntan lotions, bio-active nanoparticles)
Biosynthesis (also known as green synthesis) of nanoparticles; to be considered for publication, research papers in this area must be directed principally towards biomedical research and especially if they encompass in vivo models or proofs of concept. We welcome papers that are application-orientated or offer new concepts of substantial biomedical importance
Techniques for probing cell physiology, cell adhesion sites and cell-cell communication
Molecular self-assembly, including concepts of supramolecular chemistry, molecular recognition, and DNA nanotechnology
Societal issues such as health and the environment
Special issues. Call for papers:
Smart Nanobiosensors for Next-generation Biomedical Applications - https://digital-library.theiet.org/files/IET_NBT_CFP_SNNBA.pdf
Selected extended papers from the International conference of the 19th Asian BioCeramic Symposium - https://digital-library.theiet.org/files/IET_NBT_CFP_ABS.pdf
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