Physicochemical properties, anticancer and antimicrobial activities of metallic nanoparticles green synthesized by Aspergillus kambarensis

IF 3.8 4区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

IET nanobiotechnology Pub Date : 2021-11-23 DOI:10.1049/nbt2.12070

Mohammadhassan Gholami-Shabani, Fattah Sotoodehnejadnematalahi, Masoomeh Shams-Ghahfarokhi, Ali Eslamifar, Mehdi Razzaghi-Abyaneh

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引用次数: 4

Abstract

In the present study, metal and metal oxide nanoparticles were successfully synthesized using Aspergillus kambarensis. UV–Vis spectroscopy showed maximum absorbance of 417 nm for silver (AgNPs), 542 nm for gold (AuNPs), 582 nm for copper (CuNPs) and 367 nm for zinc oxide (ZnONPs) nanoparticles. Fourier transform infrared spectroscopy indicated the presence of various mycochemicals with diverse functional groups in the fungal cell-free filtrate. Transmission electron microscopy revealed mono and poly dispersed particles with an estimate size of 50 nm and different shapes for synthesized manufacture metallic nanoparticles (MNPs. Dynamic light scattering confirmed that MNPs were dispersed in the size range less than 50 nm. Zeta potential analysis showed values of −41.32 mV (AgNPs), −41.26 mV (AuNPs), −34.74 mV (CuNPs) and 33.72 mV (ZnONPs). X-ray diffraction analysis demonstrated crystalline nature for MNPs. All the synthesized MNPs except AuNPs showed strong antifungal and antibacterial activity in disc diffusion assay with growth inhibition zones of 13.1–44.2 mm as well as anticancer activity against HepG-2 cancer cell line with IC₅₀ in the range of 62.01–77.03 µg/ml. Taken together, the results show that biologically active MNPs synthesized by A. kambarensis for the first time could be considered as promising antimicrobial and anticancer agents for biomedical applications.

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由坎巴曲霉合成的金属纳米绿色颗粒的理化性质及其抗癌和抗菌活性

本研究成功地利用坎巴曲霉合成了金属和金属氧化物纳米颗粒。紫外可见光谱显示，纳米银(AgNPs)、金(AuNPs)、铜(CuNPs)和氧化锌(ZnONPs)的最大吸光度分别为417 nm、542 nm、582 nm和367 nm。傅里叶变换红外光谱分析表明，真菌无细胞滤液中存在多种具有不同官能团的真菌化学物质。透射电子显微镜显示了单分散和多分散的颗粒，估计尺寸为50 nm，形状不同。动态光散射证实MNPs分散在小于50 nm的尺寸范围内。Zeta电位分析结果显示，AgNPs为- 41.32 mV, AuNPs为- 41.26 mV, CuNPs为- 34.74 mV, ZnONPs为33.72 mV。x射线衍射分析证实了MNPs的结晶性质。除AuNPs外，所合成的MNPs均表现出较强的抗真菌和抗菌活性，生长抑制区为13.1 ~ 44.2 mm，对HepG-2癌细胞的抑癌活性在62.01 ~ 77.03µg/ml之间。综上所述，首次由kambarensis合成的具有生物活性的MNPs可被认为是有前景的生物医学抗菌和抗癌药物。

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来源期刊

IET nanobiotechnology 工程技术-纳米科技

CiteScore

6.20

自引率

4.30%

发文量

审稿时长

1 months

期刊介绍： Electrical and electronic engineers have a long and illustrious history of contributing new theories and technologies to the biomedical sciences. This includes the cable theory for understanding the transmission of electrical signals in nerve axons and muscle fibres; dielectric techniques that advanced the understanding of cell membrane structures and membrane ion channels; electron and atomic force microscopy for investigating cells at the molecular level. Other engineering disciplines, along with contributions from the biological, chemical, materials and physical sciences, continue to provide groundbreaking contributions to this subject at the molecular and submolecular level. Our subject now extends from single molecule measurements using scanning probe techniques, through to interactions between cells and microstructures, micro- and nano-fluidics, and aspects of lab-on-chip technologies. The primary aim of IET Nanobiotechnology is to provide a vital resource for academic and industrial researchers operating in this exciting cross-disciplinary activity. We can only achieve this by publishing cutting edge research papers and expert review articles from the international engineering and scientific community. To attract such contributions we will exercise a commitment to our authors by ensuring that their manuscripts receive rapid constructive peer opinions and feedback across interdisciplinary boundaries. IET Nanobiotechnology covers all aspects of research and emerging technologies including, but not limited to: Fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale (including methods that employ electrokinetic, electrohydrodynamic, and optical trapping techniques) Micromachining and microfabrication tools and techniques applied to the top-down approach to nanobiotechnology Nanomachining and nanofabrication tools and techniques directed towards biomedical and biotechnological applications (e.g. applications of atomic force microscopy, scanning probe microscopy and related tools) Colloid chemistry applied to nanobiotechnology (e.g. cosmetics, suntan lotions, bio-active nanoparticles) Biosynthesis (also known as green synthesis) of nanoparticles; to be considered for publication, research papers in this area must be directed principally towards biomedical research and especially if they encompass in vivo models or proofs of concept. We welcome papers that are application-orientated or offer new concepts of substantial biomedical importance Techniques for probing cell physiology, cell adhesion sites and cell-cell communication Molecular self-assembly, including concepts of supramolecular chemistry, molecular recognition, and DNA nanotechnology Societal issues such as health and the environment Special issues. Call for papers: Smart Nanobiosensors for Next-generation Biomedical Applications - https://digital-library.theiet.org/files/IET_NBT_CFP_SNNBA.pdf Selected extended papers from the International conference of the 19th Asian BioCeramic Symposium - https://digital-library.theiet.org/files/IET_NBT_CFP_ABS.pdf