IET nanobiotechnology最新文献_第6页

The inhibition of ORMDL3 prevents Alzheimer's disease through ferroptosis by PERK/ATF4/HSPA5 pathway ORMDL3通过PERK/ATF4/HSPA5途径抑制铁下垂预防阿尔茨海默病

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2023-01-20 DOI: 10.1049/nbt2.12113

Yankun Shao, Yilin Xu, Huang Di, Xinxiu Shi, Yingying Wang, Hongyu Liu, Lina Song

{"title":"The inhibition of ORMDL3 prevents Alzheimer's disease through ferroptosis by PERK/ATF4/HSPA5 pathway","authors":"Yankun Shao, Yilin Xu, Huang Di, Xinxiu Shi, Yingying Wang, Hongyu Liu, Lina Song","doi":"10.1049/nbt2.12113","DOIUrl":"10.1049/nbt2.12113","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Alzheimer's disease (AD) is a neurodegenerative disease with high incidence and widespread attention. There is currently no clear clarification of the pathogenesis. However, ORMDL3 causes ferroptosis in AD, and the potential mechanisms remain unclear. So, this study explore the function of ORMDL3 on ferroptosis in AD and its potential regulatory mechanisms. APPswe/PS1dE9 mice and C57BL/6 mice were induced into the mice model. The murine microglial BV-2 cells also were induced into the vitro model. In serum samples of AD patients, ORMDL3 mRNA expression levels were upregulated. The serum ORMDL3 levels expression was positively related to the ADL score or MoCA score in AD patients. The serum ORMDL3 expression level was positively related to MMSE score or Hcy levels in AD patients. The mRNA expression of ORMDL3 in the hippocampal tissue of the mice model of AD was upregulated at one, four and eight months. The protein expression of ORMDL3 was upregulated in the mice model of AD. ORMDL3 promoted Alzheimer's disease, and increased oxidative response and ferroptosis in a model of AD. PERK/ATF4/HSPA5 pathway is one important signal pathway for the effects of ORMDL3 in a model of AD. Collectively, these data suggested that ORMDL3 promoted oxidative response and ferroptosis in a model of AD by the PERK/ATF4/HSPA5 pathway, which might be a novel target spot mechanism of ferroptosis in AD and may serve as a regulator of AD-induced ferroptosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 3","pages":"182-196"},"PeriodicalIF":2.3,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/99/d3/NBT2-17-182.PMC10190607.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9836189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Optically driven microtools with an antibody-immobilised surface for on-site cell assembly 带有抗体固定表面的光学驱动微工具，用于现场细胞组装

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2023-01-16 DOI: 10.1049/nbt2.12114

Shuntaro Mori, Takumi Ito, Hidekuni Takao, Fusao Shimokawa, Kyohei Terao

{"title":"Optically driven microtools with an antibody-immobilised surface for on-site cell assembly","authors":"Shuntaro Mori, Takumi Ito, Hidekuni Takao, Fusao Shimokawa, Kyohei Terao","doi":"10.1049/nbt2.12114","DOIUrl":"10.1049/nbt2.12114","url":null,"abstract":"<p>To enable the accurate reproduction of organs in vitro, and improve drug screening efficiency and regenerative medicine research, it is necessary to assemble cells with single-cell resolution to form cell clusters. However, a method to assemble such forms has not been developed. In this study, a platform for on-site cell assembly at the single-cell level using optically driven microtools in a microfluidic device is developed. The microtool was fabricated by SU-8 photolithography, and antibodies were immobilised on its surface. The cells were captured by the microtool through the bindings between the antibodies on the microtool and the antigens on the cell membrane. Transmembrane proteins, CD51/61 and CD44 that facilitate cell adhesion, commonly found on the surface of cancer cells were targeted. The microtool containing antibodies for CD51/61 and CD44 proteins was manipulated using optical tweezers to capture HeLa cells placed on a microfluidic device. A comparison of the adhesion rates of different surface treatments showed the superiority of the antibody-immobilised microtool. The assembly of multiple cells into a cluster by repeating the cell capture process is further demonstrated. The geometry and surface function of the microtool can be modified according to the cell assembly requirements. The platform can be used in regenerative medicine and drug screening to produce cell clusters that closely resemble tissues and organs in vivo.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 3","pages":"197-203"},"PeriodicalIF":2.3,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/78/NBT2-17-197.PMC10190638.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9481291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre-insertion and post-insertion methods for brain delivery in normal mice 谷胱甘肽聚乙二醇化纳米脂质体多柔比星在正常小鼠脑内的生物分布比较

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2023-01-03 DOI: 10.1049/nbt2.12111

Amin Mehrabian, Saba Dadpour, Mohammad Mashreghi, Javad Zarqi, Anis Askarizadeh, Ali Badiee, Leila Arabi, Seyedeh Alia Moosavian, Mahmoud Reza Jaafari

{"title":"The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre-insertion and post-insertion methods for brain delivery in normal mice","authors":"Amin Mehrabian, Saba Dadpour, Mohammad Mashreghi, Javad Zarqi, Anis Askarizadeh, Ali Badiee, Leila Arabi, Seyedeh Alia Moosavian, Mahmoud Reza Jaafari","doi":"10.1049/nbt2.12111","DOIUrl":"10.1049/nbt2.12111","url":null,"abstract":"<p>Several obstacles limit the efficacy of brain tumour treatment, most notably the blood-brain barrier (BBB), which prevents the brain uptake of the majority of accessible medicines due to tight junctions. The presence of glutathione (GSH) receptors on the BBB surface has been demonstrated in numerous papers; consequently, products containing glutathione as a targeting ligand coupled with nanoliposomes are used to enhance drug delivery across the BBB. Here, the 5% pre-inserted PEG2000-GSH PEGylated liposomal doxorubicin was conducted according to 2B3-101 being tested in clinical trials. In addition, PEGylated nanoliposomal doxorubicin connected to the spacer-GSH targeting ligand (GSGGCE) and the PEG3400 was conducted using post-insertion method. Next, in vivo biodistribution of the produced formulations was tested on healthy mice to see if GSGGCE, as the targeted ligand, could cross the BBB compared to 5% pre-inserted PEG2000-GSH and Caelyx<sup>®</sup>. Compared to the pre-inserted formulation and Caelyx<sup>®</sup>, the post-inserted formulations' concentration was lower in the heart and higher in brain tissues, resulting in boosting the brain concentration of accumulated doxorubicin with fewer possible side effects, including cardiotoxicity. In comparison to the pre-insertion procedure, the post-insertion method is easier, faster, and more cost-effective. Moreover, employing PEG3400 and the post-insertion approach in the PEG3400-GSGGCE liposomal formulations was found to be effective in crossing the BBB.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 2","pages":"112-124"},"PeriodicalIF":2.3,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9332626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Photothermal effects of gold nanorods in aqueous solution and gel media: Influence of particle size and excitation wavelength 金纳米棒在水溶液和凝胶介质中的光热效应:粒径和激发波长的影响

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-12-21 DOI: 10.1049/nbt2.12110

Zendesha S. Mbalaha, David J. S. Birch, Yu Chen

{"title":"Photothermal effects of gold nanorods in aqueous solution and gel media: Influence of particle size and excitation wavelength","authors":"Zendesha S. Mbalaha, David J. S. Birch, Yu Chen","doi":"10.1049/nbt2.12110","DOIUrl":"10.1049/nbt2.12110","url":null,"abstract":"<p>Gold nanorods (GNRs) have emerged as the most efficient photothermal agent in cancer therapy and photocatalysis. Understanding the influence of the surrounding medium, particle size, and excitation wavelength is critical to optimising the photothermal conversion rate. Here, three pairs of large and small gold nanorods of different aspect ratios and their heat generation under laser radiation at on and off surface plasmon resonance wavelengths in aqueous solution and gel-like media are investigated. In the aqueous solution, the temperature rise of the large gold nanorods is more than with small gold nanorods at resonance excitation. In contrast to the large gold nanorods (LGNRs), the small gold nanorods (SGNRs) were less sensitive to excitation wavelength. At off-resonance excitation, the temperature rise of the SGNRs is larger than that of the LGNRs. In the agarose gel, the photothermal effect of the SGNRs is greater than LGNRs excited at the wavelength near their solution phase longitudinal surface plasmon resonance wavelength. The temperature increase of LGNRs in gel is significantly less than in aqueous solution. These findings suggest that SGNRs could be more beneficial than the LGNRs for photothermal applications in biological systems and provides further insight when selecting GNRs.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 2","pages":"103-111"},"PeriodicalIF":2.3,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9338125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microelectromechanical system-based biosensor for label-free detection of human cytomegalovirus 基于微机电系统的人巨细胞病毒无标记检测生物传感器

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-12-20 DOI: 10.1049/nbt2.12109

Khalid E. Alzahrani, Abdulaziz K. Assaifan, Mahmoud Al-Gawati, Abdullah M. Alswieleh, Hamad Albrithen, Abdullah Alodhayb

{"title":"Microelectromechanical system-based biosensor for label-free detection of human cytomegalovirus","authors":"Khalid E. Alzahrani, Abdulaziz K. Assaifan, Mahmoud Al-Gawati, Abdullah M. Alswieleh, Hamad Albrithen, Abdullah Alodhayb","doi":"10.1049/nbt2.12109","DOIUrl":"10.1049/nbt2.12109","url":null,"abstract":"<p>The human cytomegalovirus (HCMV) is an asymptomatic common virus that is typically harmless, but in some cases, it can be life threatening. Thus, there is an urgent need to develop novel diagnostic methods and strengthen the efforts to combat this virus. A microcantilever-based biosensor functionalised with the UL83-antibody of HCMV (UL83-HCMV antibody) has been developed to detect the UL83-antigen of HCMV (UL83-HCMV antigen) at different concentrations ranging from 0.3 to 300 ng/ml. The response of the biosensor to the presence of UL83-HCMV antigen was measured through the shift in resonance frequency before and after antigen–antibody binding. The system shows a low detection limit of 84 pg/ml, which is comparable to traditional sensors, and a detection time of less than 15 min was achieved. The selectivity of the sensor was demonstrated using three different proteins with and without the UL83-HCMV antigen. The biosensor shows high selectivity for the UL83-HCMV antigen. Mass loading by the UL83-HCMV antigen was roughly estimated with a sensitivity of ∼30 fg/Hz. This technique is crucial for the fabrication of portable and low-cost biosensors that can be used in real-time monitoring and enables early medical diagnosis.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 1","pages":"32-39"},"PeriodicalIF":2.3,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Formulation of nanoemulsion carriers containing Pennyroyal (Mentha pulegium) and Gijavash (Froriepia subpinnata) essential oils for enriching Doogh (Iranian dairy drink) 含有薄荷(Mentha pulegium)和吉贾瓦什(Froriepia subpinnata)精油的纳米乳载体的配方，用于强化Doogh(伊朗乳制品饮料)

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-12-07 DOI: 10.1049/nbt2.12106

Marzieh Habibvand, Mahsa Yousefi, Salar Ali Ahmed, Hamed Hassanzadeh

{"title":"Formulation of nanoemulsion carriers containing Pennyroyal (Mentha pulegium) and Gijavash (Froriepia subpinnata) essential oils for enriching Doogh (Iranian dairy drink)","authors":"Marzieh Habibvand, Mahsa Yousefi, Salar Ali Ahmed, Hamed Hassanzadeh","doi":"10.1049/nbt2.12106","DOIUrl":"10.1049/nbt2.12106","url":null,"abstract":"<p>Today, the increasing use of chemical preservatives in foods is considered one of the main problems in food industries. This study aimed to produce the pasteurised Doogh (Iranian yogurt drink) containing a nanoemulsion of essential oil (EO) with appropriate quality. A factorial test based on a completely randomised design with two treatments in three levels, including EO type (pennyroyal, Gijavash, and their equal combination) and a control sample was applied to assess the physicochemical and sensory properties of Doogh. The highest negative zeta potential and antioxidant activity percentage were observed in the sample containing the nanoemulsion of pennyroyal and enriched with a combination of two essential oils. The microbial evaluation results indicated that the total microorganism count was minimised in the Doogh containing the nanoemulsion of Gijavash. The nanoemulsions of pennyroyal and Gijavash can be added into Doogh formulation to produce a new product with maximum sensory acceptability.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 2","pages":"80-90"},"PeriodicalIF":2.3,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0a/00/NBT2-17-80.PMC10116015.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9346339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trace elements-based Auroshell gold@hematite nanostructure: Green synthesis and their hyperthermia therapy 基于微量元素的aurroshell gold@hematite纳米结构:绿色合成及其热疗

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-11-24 DOI: 10.1049/nbt2.12107

Hadil M. Alahdal, Sumya Ayad Abdullrezzaq, Hawraz Ibrahim M. Amin, Sitah F. Alanazi, Abduladheem Turki Jalil, Mehrdad Khatami, Marwan Mahmood Saleh

{"title":"Trace elements-based Auroshell gold@hematite nanostructure: Green synthesis and their hyperthermia therapy","authors":"Hadil M. Alahdal, Sumya Ayad Abdullrezzaq, Hawraz Ibrahim M. Amin, Sitah F. Alanazi, Abduladheem Turki Jalil, Mehrdad Khatami, Marwan Mahmood Saleh","doi":"10.1049/nbt2.12107","DOIUrl":"10.1049/nbt2.12107","url":null,"abstract":"<p>Hyperthermia is an additional treatment method to radiation therapy/chemotherapy, which increases the survival rate of patients without side effects. Nowadays, Auroshell nanoparticles have attracted much attention due to their precise control over heat use for medical purposes. In this research, iron/gold Auroshell nanoparticles were synthesised using green nanotechnology approach. Auroshell gold@hematite nanoparticles were synthesised and characterised with rosemary extract in one step and the green synthesised nanoparticles were characterised by X-ray powder diffraction, SEM, high-resolution transmission electron microscopy, and X-ray photoelectron spectroscopy analysis. Cytotoxicity of Auroshell iron@gold nanoparticles against normal HUVEC cells and glioblastoma cancer cells was evaluated by 2,5-diphenyl-2H-tetrazolium bromide method, water bath hyperthermia, and combined method of water bath hyperthermia and nano-therapy. Auroshell gold@hematite nanoparticles with minimal toxicity are safe against normal cells. The gold shell around the magnetic core of magnetite caused the environmental and cellular biocompatibility of these Auroshell nanoparticles. These magnetic nanoparticles with targeted control and transfer to the tumour tissue led to uniform heating of malignant tumours as the most efficient therapeutic agent.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 1","pages":"22-31"},"PeriodicalIF":2.3,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c8/fa/NBT2-17-22.PMC9932437.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9290384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The anti-cancer effect of chitosan/resveratrol polymeric nanocomplex against triple-negative breast cancer; an in vitro assessment 壳聚糖/白藜芦醇聚合物纳米复合物对三阴性乳腺癌的抗癌作用体外评估

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-11-24 DOI: 10.1049/nbt2.12108

Azam Bozorgi, Zahra Haghighi, Mohammad Rasool Khazaei, Maryam Bozorgi, Mozafar Khazaei

{"title":"The anti-cancer effect of chitosan/resveratrol polymeric nanocomplex against triple-negative breast cancer; an in vitro assessment","authors":"Azam Bozorgi, Zahra Haghighi, Mohammad Rasool Khazaei, Maryam Bozorgi, Mozafar Khazaei","doi":"10.1049/nbt2.12108","DOIUrl":"10.1049/nbt2.12108","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Herein, the authors synthesised chitosan nanoparticles (Cs NPs) as a resveratrol (RSV) carrier and evaluated their efficacy in stimulating apoptosis in MDA-MB 231 cells. Blank (Cs NPs) and RSV- Cs NPs (RSV-Cs NPs) were synthesised via ionic gelation and characterised by using fourier-transform infrared spectroscopy (FTIR), Scanning electron microscope, dynamic light scattering/Zeta potential and RSV release. MDA-MB 231 cells were treated with RSV, Cs NPs and RSV-Cs NPs (24, 48, and 72 h), followed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Cell toxicity was evaluated using lactate dehydrogenase assay, and real-time polymerase chain reaction was performed to explore apoptosis induction. FTIR spectra confirmed the NPs via the formation of cross-linking bonds. Cs and RSV-Cs NPs sizes were about 75 and 198 nm with 14 and 24 mV zeta potentials. The RSV entrapment efficiency was 52.34 ± 0.16%, with an early rapid release followed by a sustained manner. Cs and RSV-Cs NPs inhibited cell proliferation at lower concentrations and IC50 values. RSV-Cs NPs had the most cytotoxic effect and stimulated intrinsic apoptotic pathway, indicated by increased Bcl-2-associated x (BAX), BAX/Bcl-2 ratio, P53 expressions, reduced Bcl-2 and upregulated caspases 3, 8 and 9. RSV-Cs NPs have a great potential to suppress invasive breast cancer cell proliferation by targeting mitochondrial metabolism and inducing the intrinsic apoptotic pathway.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 2","pages":"91-102"},"PeriodicalIF":2.3,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/e2/NBT2-17-91.PMC10116016.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9388524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Extraction of alprazolam in biological samples using the dispersive solid-phase method with nanographene oxide grafted with α-pyridylamine α-吡啶胺接枝纳米氧化石墨烯分散固相法提取生物样品中的阿普唑仑

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-11-23 DOI: 10.1049/nbt2.12105

Morteza Parsayi Arvand, Ali Moghimi, Milad Abniki

引用次数: 1

Biosynthesis of gold nanoparticles in the fruiting body of enoki mushrooms (Flammulina velutipes) under Pb2+ induction Pb2+诱导下金针菇子实体中金纳米粒子的生物合成

IF 2.3 4区工程技术

IET nanobiotechnology Pub Date : 2022-11-19 DOI: 10.1049/nbt2.12104

Jingang Mo, Jun Jin, Han Yu, Mingjun Ai, Die Hu, Linlin Li, Kai Song

{"title":"Biosynthesis of gold nanoparticles in the fruiting body of enoki mushrooms (Flammulina velutipes) under Pb2+ induction","authors":"Jingang Mo, Jun Jin, Han Yu, Mingjun Ai, Die Hu, Linlin Li, Kai Song","doi":"10.1049/nbt2.12104","DOIUrl":"https://doi.org/10.1049/nbt2.12104","url":null,"abstract":"<p>Fungi can produce many compounds, such as proteins, enzymes, amino acids, and polysaccharides, which are internalised and enriched for metals, and are widely used as reducing and stabilising agents for the biosynthesis of gold nanoparticles (Au NPs). Almost all fungal sources used in the synthesis of the Au NPs are in the form of cell filtrates or mycelial suspensions. However, the culture of cell-free fungal filtrate and mycelium is not comparable to the propagation of fungal substrates in input and operation. Here, we evaluated in vivo biosynthesis of Au NPs in enoki mushrooms (<i>Flammulina velutipes</i>). HAuCl<sub>4</sub> was reduced in the fruiting body of the enoki mushrooms via induction by Pb<sup>2+</sup>, resulting in the generation of Au NPs. We then employed UV-Vis absorption spectroscopy, Transmission Electron Microscope, and Energy Dispersive Spectrometer to characterise various shapes of the Au NPs. The elemental analysis indicated that the Au NPs were mainly concentrated in organelles of the stalk and cap cells. We also demonstrated that 0.3–0.5 mM HAuCl<sub>4</sub> was the optimal stress treatment concentration based on the changes in physiological indicators of the enoki mushrooms. This work reveals that fungi can be utilised well as nanomaterial bioreactors.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 2","pages":"61-68"},"PeriodicalIF":2.3,"publicationDate":"2022-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50152462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0