Immunological Investigations最新文献_第3页

3D Bioprinting of Pig Macrophages and Human Cells Discovered the P2Y14 Receptor as a Mediator of Xenogenic Immune Responses. 猪巨噬细胞和人类细胞的三维生物打印发现了作为异种免疫反应介质的 P2Y14 受体。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-02 DOI: 10.1080/08820139.2024.2411388

Hyungkuen Kim, Sung-Jo Kim

{"title":"3D Bioprinting of Pig Macrophages and Human Cells Discovered the P2Y14 Receptor as a Mediator of Xenogenic Immune Responses.","authors":"Hyungkuen Kim, Sung-Jo Kim","doi":"10.1080/08820139.2024.2411388","DOIUrl":"https://doi.org/10.1080/08820139.2024.2411388","url":null,"abstract":"Background: The survival rate of pig lung xenotransplantation (PLXTx) recipients is severely limited by intense xenogenic immune responses, necessitating further insights into xenogeneic immunity and the development of models to study the PLXTx immune response.Methods: We identified regulators of PLXTx immune response Using Gene ontology analysis. We assessed the metabolic changes and protein levels in 3D4/31 pig alveolar macrophages (PAMs) through flow cytometry and immunoblotting. To induce a xenogenic immune response, we co-cultured 3D4/31-PAMs with A549 human alveolar epithelial cells and evaluated cytokine expression using qRT-PCR.Results: Gene ontology analysis identified STAT1 and alveolar macrophages as contributors to lung autoimmunity and transplant rejection. In 3D4/31-PAMs, phorbol myristate acetate-induced glycogen accumulation and cyclooxygenase-2 expression were inhibited by the P2Y14 inhibitor PPTN. Co-culturing 3D4/31-PAMs with A549 human alveolar epithelial cells via 3D bioprinting resulted in a more pronounced inflammatory response than 2D co-culture, with increased expression of genes related to the P2Y14 cascade and inflammation. This inflammatory gene expression was prevented by PPTN treatment.Conclusion: Based on these results, we propose alginate bioprinting as an in vitro model for PLXTx and suggest that P2Y14 is a key regulator of xenogeneic immune responses in PAMs.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-16"},"PeriodicalIF":2.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Serum Markers that Distinguish Behcet's Disease from Idiopathic Recurrent Aphthous Stomatitis. 区分白塞氏病和特发性复发性口疮的新型血清标记物

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-02 DOI: 10.1080/08820139.2024.2410743

Mengya Zhu, Xinliang Mao, Xianqian Huang, Minzhi Gan, Keyue Zhang, Yong Chen

{"title":"Novel Serum Markers that Distinguish Behcet's Disease from Idiopathic Recurrent Aphthous Stomatitis.","authors":"Mengya Zhu, Xinliang Mao, Xianqian Huang, Minzhi Gan, Keyue Zhang, Yong Chen","doi":"10.1080/08820139.2024.2410743","DOIUrl":"https://doi.org/10.1080/08820139.2024.2410743","url":null,"abstract":"Background: Behcet's disease (BD) is a rare and recurrent autoinflammatory disorder characterized by systemic vasculitis, frequently manifested as recurrent aphthous stomatitis (RAS). We aim to identify specific serum proteins to discriminate between BD and idiopathicRAS.Method: Peripheral blood was collected from 12 BD patients, 12 idiopathic RAS patients, and 21 healthy volunteers. The serum samples underwent Tandem Mass Tag-based mass spectrometry analysis. Differentially expressed proteins (DEPs) were identified for KEGG pathway enrichment, Gene Ontology (GO), and protein-protein interaction (PPI) analyses. ELISA was utilized to verify two BD-specific DEPs in another cohort consisting of 18 BD patients, 18 idiopathic RAS patients, and 18 controls.Results: Compared with RAS serum, BD serum showed 242 DEPs. 49 proteins were differentially expressed in BD but not RAS serum compared to healthy controls. KEGG pathway and GO analyses revealed that DEPs in BD and RAS have similar biological functions and cellular distributions, featuring a significant association with pathways regulating blood coagulation and immune response. When comparing DEPs between BD and RAS, several keratins emerged as markers that distinguish RAS from BD. We also identified multiple DEPs in BD but not RAS patients. PPI analysis uncovered that lipoprotein metabolism regulators serve as hub proteins, indicating their potentially essential roles in BD pathology. In addition, ELISA results confirmed the elevated LRG1 and SOD3 levels in BD, but not RAS patients, compared to healthy donors.Conclusion: Our data uncovered novel serum proteins that distinguish BD from RAS, which may potentially be useful in BD diagnosis and treatment.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-17"},"PeriodicalIF":2.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis. 类风湿关节炎患者对甲氨蝶呤治疗差异反应的 T 细胞受体汇聚特征

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI: 10.1080/08820139.2024.2381078

Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng

{"title":"Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis.","authors":"Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng","doi":"10.1080/08820139.2024.2381078","DOIUrl":"10.1080/08820139.2024.2381078","url":null,"abstract":"Background: Methotrexate (MTX) serves as the initial treatment for rheumatoid arthritis (RA). However, a substantial proportion of RA patients, estimated between 30% and 50%, do not respond positively to MTX. While the T-cell receptor (TCR) is crucial for the immune response during RA, its role in differentiating MTX responsiveness has not been thoroughly investigated.Methods: This study used next-generation sequencing to analyze the TCR β-chain complementary determining region sequences in peripheral blood mononuclear cells obtained from RA patients before MTX treatment. This study aimed to compare the characteristics of the TCR repertoire between the MTX responder and non-responder groups.Results: The study identified a significant difference in the TRBV6-6 gene (p = .003) concerning MTX treatment response. Additionally, a significant difference was found in the number of \"3\" nucleotide deletions at the 5'Jdels site (p = .023) in the VDJ rearrangement.Conclusion: These findings suggest distinct TCR repertoire characteristics between MTX responder and non-responder groups among RA patients. This discovery offers new insights into understanding the variable responses of RA patients to MTX therapy.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1113-1124"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM). 原代小鼠骨髓衍生巨噬细胞（BMDM）的收集、分化、冷冻保存和复苏综合方案。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1080/08820139.2024.2382805

Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple

{"title":"A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM).","authors":"Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple","doi":"10.1080/08820139.2024.2382805","DOIUrl":"10.1080/08820139.2024.2382805","url":null,"abstract":"Background: The field of immunology has undoubtedly benefited from the in vitro use of cell lines for immunological studies; however, due to the \"immortal\" nature of many cell lines, they are not always the best model. Thus, direct collection and culture of primary cells from model organisms is a solution that many researchers utilize. To the best of our knowledge, there is not a singular protocol which encompasses the entire process of bone marrow cell collection through cryopreservation and resuscitation of cells from a murine model.Methods: Bone marrow cells were collected from mice with a C57BL6 genetic background. Cells were differentiated using L929 conditioned media. Cells were assessed using a combination of microscopy, differential staining, immunocytochemistry, and trypan blue. Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear S. aureus.Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear S. aureus.Conclusion: Crypopreserved and resuscitated primary murine BMDMs were viable and retained their pverall S. aureus clearance ability.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1001-1012"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predominance of CD137⁺ And TNF-α Expressing CD8⁺ Central Memory T Cells in Mild COVID-19 Recovered Patients Upon SARS-CoV-2 Re-Exposure. 再次接触SARS-CoV-2病毒后，轻度COVID-19康复者体内CD137+和TNF-α表达的CD8+中央记忆T细胞占主导地位

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-07-12 DOI: 10.1080/08820139.2024.2376003

Rephany Fonseca Peixoto, Pedro Henrique de Sousa Palmeira, Bárbara Guimarães Csordas, Luiz Henrique Agra Cavalcante-Silva, Arthur Gomes de Andrade, Isac Almeida de Medeiros, Fátima de Lourdes Assunção Araújo de Azevedo, Robson Cavalcante Veras, Daniele Janebro, Ian P G Do Amaral, Tatjana Souza Lima Keesen

{"title":"Predominance of CD137+ And TNF-α Expressing CD8+ Central Memory T Cells in Mild COVID-19 Recovered Patients Upon SARS-CoV-2 Re-Exposure.","authors":"Rephany Fonseca Peixoto, Pedro Henrique de Sousa Palmeira, Bárbara Guimarães Csordas, Luiz Henrique Agra Cavalcante-Silva, Arthur Gomes de Andrade, Isac Almeida de Medeiros, Fátima de Lourdes Assunção Araújo de Azevedo, Robson Cavalcante Veras, Daniele Janebro, Ian P G Do Amaral, Tatjana Souza Lima Keesen","doi":"10.1080/08820139.2024.2376003","DOIUrl":"10.1080/08820139.2024.2376003","url":null,"abstract":"Introduction: Memory CD8+ T cells are essential for long-term immune protection in viral infections, including COVID-19.Methods: This study examined the responses of CD8+ TEM, TEMRA, and TCM subsets from unvaccinated individuals who had recovered from mild and severe COVID-19 by flow cytometry.Results and discussion: The peptides triggered a higher frequency of CD8+ TCM cells in the recovered mild group. CD8+ TCM and TEM cells showed heterogeneity in CD137 expression between evaluated groups. In addition, a predominance of CD137 expression in naïve CD8+ T cells, TCM, and TEM was observed in the mild recovered group when stimulated with peptides. Furthermore, CD8+ TCM and TEM cell subsets from mild recovered volunteers had higher TNF-α expression. In contrast, the expression partner of IFN-γ, IL-10, and IL-17 indicated an antiviral signature by CD8+ TEMRA cells. These findings underscore the distinct functional capabilities of each memory T cell subset in individuals who have recovered from COVID-19 upon re-exposure to SARS-CoV-2 antigens.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1092-1101"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Neuro-Immune Interactions in the Pathology and Pathogenesis of Allergic Rhinitis. 神经免疫相互作用在过敏性鼻炎病理和发病机制中的作用。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-07-23 DOI: 10.1080/08820139.2024.2382792

Ya-An Lan, Jia-Xi Guo, Min-Hua Yao, Yi-Ting Kang, Zi-Rui Liao, Yu-Hong Jing

引用次数: 0

Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors. 针对传染病中的巨噬细胞极化：M1/M2 功能图谱、免疫信号转导和微生物毒力因子。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-06-24 DOI: 10.1080/08820139.2024.2367682

Cláudio Daniel Cerdeira, Maísa R P L Brigagão

{"title":"Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors.","authors":"Cláudio Daniel Cerdeira, Maísa R P L Brigagão","doi":"10.1080/08820139.2024.2367682","DOIUrl":"10.1080/08820139.2024.2367682","url":null,"abstract":"Introduction: An event of increasing interest during host-pathogen interactions is the polarization of patrolling/naive monocytes (MOs) into macrophage subsets (MФs). Therapeutic strategies aimed at modulating this event are under investigation.Methods: This review focuses on the mechanisms of induction/development and profile of MФs polarized toward classically proinflammatory (M1) or alternatively anti-inflammatory (M2) phenotypes in response to bacteria, fungi, parasites, and viruses.Results and discussion: It highlights nuclear, cytoplasmic, and cell surface receptors (pattern recognition receptors/PPRs), microenvironmental mediators, and immune signaling. MФs polarize into phenotypes: M1 MФs, activated by IFN-γ, pathogen-associated molecular patterns (PAMPs, e.g. lipopolysaccharide) and membrane-bound PPRs ligands (TLRs/CLRs ligands); or M2 MФs, induced by interleukins (ILs-4, -10 and -13), antigen-antibody complexes, and helminth PAMPs. Polarization toward M1 and M2 profiles evolve in a pathogen-specific manner, with or without canonicity, and can vary widely. Ultimately, this can result in varying degrees of host protection or more severe disease outcome. On the one hand, the host is driving effective MФs polarization (M1 or M2); but on the other hand, microorganisms may skew the polarization through virulence factors to increase pathogenicity. Cellular/genomic reprogramming also ensures plasticity of M1/M2 phenotypes. Because modulation of polarization can occur at multiple points, new insights and emerging perspectives may have clinical implications during the inflammation-to-resolution transition; translated into practical applications as for therapeutic/vaccine design target to boost microbicidal response (M1, e.g. triggering oxidative burst) with specifics PAMPs/IFN-γ or promote tissue repair (M2, increasing arginase activity) via immunotherapy.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1030-1091"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma Extracellular Vesicles Derived from Pediatric COVID-19 Patients Modulate Monocyte and T Cell Immune Responses Based on Disease Severity. 从小儿 COVID-19 患者体内提取的血浆细胞外小泡可根据疾病严重程度调节单核细胞和 T 细胞免疫反应。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1080/08820139.2024.2385992

Pınar Gur Cetinkaya, Irem Fatma Abras, Irem Evcili, Tugçe Yildirim, Yasemin Ceylan, Fehime Kara Eroglu, Başak Kayaoglu, Emre Mert İpekoglu, Aysegul Akarsu, Muzaffer Yıldırım, Tamer Kahraman, Ali Bülent Cengiz, Umit Murat Sahiner, Bulent Enis Sekerel, Yasemin Ozsurekci, Ozge Soyer, Ihsan Gursel

{"title":"Plasma Extracellular Vesicles Derived from Pediatric COVID-19 Patients Modulate Monocyte and T Cell Immune Responses Based on Disease Severity.","authors":"Pınar Gur Cetinkaya, Irem Fatma Abras, Irem Evcili, Tugçe Yildirim, Yasemin Ceylan, Fehime Kara Eroglu, Başak Kayaoglu, Emre Mert İpekoglu, Aysegul Akarsu, Muzaffer Yıldırım, Tamer Kahraman, Ali Bülent Cengiz, Umit Murat Sahiner, Bulent Enis Sekerel, Yasemin Ozsurekci, Ozge Soyer, Ihsan Gursel","doi":"10.1080/08820139.2024.2385992","DOIUrl":"10.1080/08820139.2024.2385992","url":null,"abstract":"Background: The COVID-19 pandemic has caused significant morbidity and mortality globally. The role of plasma-derived extracellular vesicles (EVs) in pediatric COVID-19 patients remains unclear.Methods: We isolated EVs from healthy controls (n = 13) and pediatric COVID-19 patients (n = 104) with varying severity during acute and convalescent phases using serial ultracentrifugation. EV effects on healthy PBMCs, naïve CD4+ T cells, and monocytes were assessed through in vitro assays, flow cytometry, and ELISA.Results: Our findings indicate that COVID-19 severity correlates with diverse immune responses. Severe acute cases exhibited increased cytokine levels, decreased IFNγ levels, and lower CD4+ T cell and monocyte counts, suggesting immunosuppression. EVs from severe acute patients stimulated healthy cells to express higher PDL1, increased Th2 and Treg cells, reduced IFNγ secretion, and altered Th1/Th17 ratios. Patient-derived EVs significantly reduced proinflammatory cytokine production by monocytes (p < .001 for mild, p = .0025 for severe cases) and decreased CD4+ T cell (p = .043) and monocyte (p = .033) populations in stimulated healthy PBMCs.Conclusion: This study reveals the complex relationship between immunological responses and EV-mediated effects, emphasizing the impact of COVID-19 severity. We highlight the potential role of plasma-derived EVs in early-stage immunosuppression in severe COVID-19 patients.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1141-1175"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protection of Astragalus Polysaccharide in BALB/C Mice during Brucella melitensis M5 Infection. 黄芪多糖对BALB/C小鼠M5布鲁氏菌感染的保护作用

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-29 DOI: 10.1080/08820139.2024.2380718

Yuanqiang Zheng, Yajing Chen, Jianlong Zhao, Meihua Wu, Ligao Bao, Dantong Zhao, Shuang Bai, Dongdong Di, Yanchun Shi

{"title":"The Protection of Astragalus Polysaccharide in BALB/C Mice during Brucella melitensis M5 Infection.","authors":"Yuanqiang Zheng, Yajing Chen, Jianlong Zhao, Meihua Wu, Ligao Bao, Dantong Zhao, Shuang Bai, Dongdong Di, Yanchun Shi","doi":"10.1080/08820139.2024.2380718","DOIUrl":"10.1080/08820139.2024.2380718","url":null,"abstract":"Introduction: Brucellosis is an important zoonosis worldwide, affecting humans and animals. There are no specific medicines available to treat brucellosis. Astragalus polysaccharide (APS) is derived from Astragalus membranaceus and exhibits impressive bioactivity, including anti-aging, anti-tumor, and immunomodulatory functions.Methods: Mice were intraperitoneally inoculated with Brucella melitensis M5 and then treated with APS intraperitoneally injection daily for 7 d.Results: Compared to the M5-infected group, the lower bacteria loads in the APS-treated groups were proved, especially at the acute stage of infection. APS treatment relieved splenomegaly, excess expressions of several pro-inflammatory cytokines (including CXCL1, IFN-γ, IL-1β, IL-2, IL-12p70, and TNF-α). The raised level of IL-4 was observed in APS-treated mice. APS contributed to raising the ratio of M1 macrophage and reducing the ratio of M2 macrophage in the blood.Discussion: The present study provides some evidence on the potential application of APS in controlling and treating brucellosis and should be further explored.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1102-1112"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase in Mitochondrial Mass of Lymphocyte Subsets in Anti-MDA5 and TIF1-γ-Positive Dermatomyositis Patients. 抗 MDA5 和 TIF1-γ 阳性皮肌炎患者淋巴细胞亚群线粒体质量的增加

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-18 DOI: 10.1080/08820139.2024.2402824

Xiaomeng Li,Qingqing Ma,Yuan Huang,Linlin Cheng,Yongmei Liu,Haolong Li,Haoting Zhan,Fengchun Zhang,Yudong Liu,Yongzhe Li

{"title":"Increase in Mitochondrial Mass of Lymphocyte Subsets in Anti-MDA5 and TIF1-γ-Positive Dermatomyositis Patients.","authors":"Xiaomeng Li,Qingqing Ma,Yuan Huang,Linlin Cheng,Yongmei Liu,Haolong Li,Haoting Zhan,Fengchun Zhang,Yudong Liu,Yongzhe Li","doi":"10.1080/08820139.2024.2402824","DOIUrl":"https://doi.org/10.1080/08820139.2024.2402824","url":null,"abstract":"OBJECTIVESThe mitochondrial function in anti-MDA5 and TIF1-γ-positive dermatomyositis (DM) is relatively unknown. This study attempted to explore mitochondrial mass within the peripheral lymphocyte subsets of anti-MDA5 and TIF1-γ-positive DM.METHODSThis cross-sectional study enrolled 109 DM patients and 32 healthy controls (HCs). The mitochondrial mass of peripheral lymphocyte subsets was analyzed via flow cytometry using median fluorescence intensity assessment.RESULTSCompared with HCs, there was an abnormal change in peripheral lymphocyte subsets in anti-MDA5 and anti-TIF1-γ-positive DM patients. Anti-MDA5 and anti-TIF1-γ-positive DM patients also exhibited a significantly elevated mitochondrial mass in peripheral lymphocyte subsets. Furthermore, anti-MDA5 antibody levels were positively associated with the mitochondrial mass of most lymphocyte subsets in anti-MDA5-positive DM patients. Univariate logistic regression analysis indicated that the increased mitochondrial mass in some peripheral lymphocyte subsets was related to the occurrence of anti-MDA5-positive DM and presence of anti-MDA5 antibodies. Similar results were obtained in anti-TIF1-γ-positive DM patients.CONCLUSIONSAbnormal lymphocyte subset counts and percentages as well as altered mitochondrial mass in anti-MDA5 and TIF1-γ-positive DM patients were associated with anti-MDA5 and TIF1-γ antibodies. We believe that these results may provide novel mitochondria-based insights into DM pathogenesis.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"4 1","pages":"1-16"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0