Immunological Investigations最新文献_第2页

Crosstalk and Prospects of TBK1 in Inflammation. TBK1 在炎症中的相互作用与前景

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-11-01 Epub Date: 2024-08-28 DOI: 10.1080/08820139.2024.2392587

Huan Liu, Qihuan Sheng, Juhua Dan, Xiaoli Xie

{"title":"Crosstalk and Prospects of TBK1 in Inflammation.","authors":"Huan Liu, Qihuan Sheng, Juhua Dan, Xiaoli Xie","doi":"10.1080/08820139.2024.2392587","DOIUrl":"10.1080/08820139.2024.2392587","url":null,"abstract":"Background: TANK-binding kinase 1 (TBK1) is a pivotal mediator of innate immunity, activated by receptors such as mitochondrial antiviral signaling protein (MAVS), stimulator of interferon genes (STING), and TIR-domain-containing adaptor inducing interferon-β (TRIF). It modulates immune responses by exerting influence on the type I interferons (IFN-Is) signaling and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways, Over the past few years, TBK1 multifaceted role in both immune and inflammatory responses is increasingly recognized.Methods and results: This review aims to scrutinize how TBK1 operates within the NF-κB pathway and the interferon regulatory transcription factor 3 (IRF3)-dependent IFN-I pathways, highlighting the kinases and other molecules involved in these processes. This analysis reveals the distinctive characteristics of TBK1's involvement in these pathways. Furthermore, it has been observed that the role of TBK1 in exerting anti-inflammatory or pro-inflammatory effects is contingent upon varying pathological conditions, indicating a multifaceted role in immune regulation.Discussion: TBK1's evolving role in various diseases and the potential of TBK1 inhibitors as therapeutic agents are explored. Targeting TBK1 may provide new strategies for treating inflammatory disorders and autoimmune diseases associated with IFN-Is, warranting further investigation.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IRF3 Promotes Asthma Pathogenesis by Regulating Type 2 Innate Lymphoid Cells. IRF3通过调节2型先天性淋巴细胞促进哮喘发病机制

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-29 DOI: 10.1080/08820139.2024.2418935

Zihao Liang, Zixin Chen, Jinwei Chen, Yunfan Zhou, Hua Chen, Meimei Gu, Dehong Yan, Qiong Yang

{"title":"IRF3 Promotes Asthma Pathogenesis by Regulating Type 2 Innate Lymphoid Cells.","authors":"Zihao Liang, Zixin Chen, Jinwei Chen, Yunfan Zhou, Hua Chen, Meimei Gu, Dehong Yan, Qiong Yang","doi":"10.1080/08820139.2024.2418935","DOIUrl":"https://doi.org/10.1080/08820139.2024.2418935","url":null,"abstract":"Background: Allergic asthma is characterized by airway hyperresponsiveness triggered by inhaled allergens. Type 2 innate lymphoid cells (ILC2s) have been demonstrated to play a crucial role in promoting airway inflammation through the secretion of type 2 effector cytokines. However, the mechanisms underlying the functions of lung ILC2s remain unclear.Methods: In this study, we investigated the expression of IRF3 in ILC2s in both human patients and mouse models of asthma. We utilized IRF3-deficient mice to assess the impact of IRF3 deficiency on ILC2 function in a model of IL33-induced asthma. Additionally, we explored the mechanisms underlying IRF3-mediated regulation of ILC2s, focusing on the involvement of the transcription factor Gata3.Results: Our findings revealed elevated expression of IRF3 in ILC2s of patients and mice with asthma, suggesting a potential role for IRF3 in the pathogenesis of allergic asthma. Furthermore, we demonstrated that IRF3 deficiency impairedthe expansion and function of ILC2s in IL33-induced asthma, highlighting the importance of IRF3 in regulating ILC2-mediated responses. Importantly, we showed that the regulation of ILC2s by IRF3 was independent of Th2 cells and mediated by the transcription factor Gata3.Conclusion: This study identifies IRF3 as a novel regulator of lung ILC2s and suggests its potential as a promising immunotherapeutic target for allergic asthma. These findings shed light on the intricate mechanisms underlying asthma pathogenesis and provide insights into potential strategies for the development of targeted therapies for this prevalent airway disease.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tectochrysin Alleviates Periodontitis by Modulating M2/M1 Macrophage Ratio and Oxidative Stress Via Nuclear Factor Kappa B/Heme Oxygenase-1/Nuclear Factor Erythroid 2-Related Factor 2 Pathway. 通过核因子 Kappa B/Heme 氧化酶-1/核因子红细胞 2 相关因子 2 途径调节 M2/M1 巨噬细胞比例和氧化应激，从而缓解牙周炎。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-29 DOI: 10.1080/08820139.2024.2418938

Ye Yin, Yanming Weng, Zeyu Ma, Li Li

{"title":"Tectochrysin Alleviates Periodontitis by Modulating M2/M1 Macrophage Ratio and Oxidative Stress Via Nuclear Factor Kappa B/Heme Oxygenase-1/Nuclear Factor Erythroid 2-Related Factor 2 Pathway.","authors":"Ye Yin, Yanming Weng, Zeyu Ma, Li Li","doi":"10.1080/08820139.2024.2418938","DOIUrl":"https://doi.org/10.1080/08820139.2024.2418938","url":null,"abstract":"Background: Tectochrysin suppresses several diseases. In this study, we aimed to explore the effects of tectochrysin ona rat model of periodontitis PDS).Methods: Male Sprague-Dawley (SD) rats were subjected to ligature to induce periodontitis. Bone parameters were analyzed using micro-computed tomography and periodontal tissues were evaluated using Masson's, hematoxylin and eosin, and tartrate-resistant acid phosphatase staining. The expression of HO-1, Nrf2, CD206, Arg-1, and iNOS was evaluated using immunohistochemistry. Malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) levels and IL-1β, IL-6, and tumor necrosis factor (TNF)-α,and NF-κB and Nrf2/HO-1 were analyzed.Results: Tectochrysin reduced alveolar bone loss, promoted new bone formation, and inhibited osteoclast formation in periodontitis rats. It decreased the number of inflammatory cells and the levels of IL-1β, IL-6, and TNF-α, indicating a reduction in inflammation. Tectochrysin restored the Arg-1/iNOS ratio, indicating M2 macrophage polarization, and inhibited the NF-kB pathway. Tectochrysin restored GSH and SOD levels, inhibited MDA content, and activated the HO-1/Nrf2 pathway.Conclusion: Tectochrysin alleviates PDS in rats by modulating the M2/M1 macrophage ratio via the NF-kB pathway and suppressing oxidative stress via the HO-1/Nrf2 pathway.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Physiological and Therapeutic Role of CD47 in Macrophage Function and Cancer. CD47 在巨噬细胞功能和癌症中的生理和治疗作用

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-17 DOI: 10.1080/08820139.2024.2415409

Shelby N Bess, Matthew J Igoe, Timothy J Muldoon

{"title":"The Physiological and Therapeutic Role of CD47 in Macrophage Function and Cancer.","authors":"Shelby N Bess, Matthew J Igoe, Timothy J Muldoon","doi":"10.1080/08820139.2024.2415409","DOIUrl":"https://doi.org/10.1080/08820139.2024.2415409","url":null,"abstract":"Background: Immunotherapy is an emerging strategy in cancer therapeutics aimed at modulating the immune system to inhibit pro-tumor pathways and increase a tumor's sensitivity to chemotherapy. Several clinically approved immunotherapy treatments, such as monoclonal antibody treatments, have been successful in solid tumors such as breast, colorectal, and pancreatic. However, an outstanding challenge of these strategies is tumor cell resistance. One target of interest for immune cell modulation is targeting macrophages that enter the tumor microenvironment. More specifically, an immune checkpoint of interest is CD47. CD47 is a transmembrane protein that inhibits phagocytic activity by acting as a \"don't eat me\" signal. In both mice and humans, healthy cells can express CD47, while solid malignancies like colorectal and breast cancer express it most strongly.Methods: Analysis of literature data on the physiological and functional roles of tissue-resident macrophages, along with the structure and mechanisms of action of the CD47 pathway was explored. We also explored how CD47 can influence different aspects of the tumor microenvironment (i.e. cellular metabolism and hypoxia) in addition to current clinical strategies and challenges associated with targeting CD47.Results: Overall, it was discovered that CD47 is overexpressed in a variety of cancer types in addition to normal tissue, making it a promising treatment regimen to enhance the capability of macrophages to phagocytose tumor cells. However, treatment efficacy is varied in pre-clinical and clinical models due to various challenges such as off-target effects.Conclusion: This review emphasizes the diverse functionality of macrophages in normal and cancerous tissue, while also emphasizing the importance of macrophage targeting and their clinical significance.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Relationship Between Serum IgE Level and IL-4 and IL-13 Cytokines in Colorectal Cancer Patients. 结直肠癌患者血清 IgE 水平与 IL-4 和 IL-13 细胞因子之间的关系

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-11 DOI: 10.1080/08820139.2024.2414091

Zahra Mozooni, Fatemeh Faraji, Sara Minaeian, Leyla Bahadorizadeh

{"title":"The Relationship Between Serum IgE Level and IL-4 and IL-13 Cytokines in Colorectal Cancer Patients.","authors":"Zahra Mozooni, Fatemeh Faraji, Sara Minaeian, Leyla Bahadorizadeh","doi":"10.1080/08820139.2024.2414091","DOIUrl":"https://doi.org/10.1080/08820139.2024.2414091","url":null,"abstract":"Background: Colorectal cancer (CRC) is the most common malignancy of the digestive system in the world. Immune cells and molecules in tumor microenvironment are crucial.Identifying immune system components in cancer aids in biomarker discovery. This study investigated the serum IgE levels and expression of IL-4 and IL-13 in the tissue and serum of CRC patients and explored their possible association with pathological and clinical factors.Materials and methods: Thirty-six patients with CRC and 36 healthy individuals were involved in the study. Tissues and blood samples were collected. Serum levels of IgE and IL-4 and IL-13 were analyzed using the ELISA method. The quantitative Real-Time PCR (qRT-PCR) technique was used to assess the expression levels of the cytokines in CRC tissue samples in comparison with the adjacent control tissue.Results: Our results revealed that the serum level of IL-4 and IL-13 and also their gene expression levels were significantly decreased in CRC patients compared to the controls. The results of this study revealed that there is no significant difference in the serum levels of IgE between CRC patients and the control group.Conclusion: All in all, the results of the current research suggest that the expression levels of IL-13, IL-4, and IgE vary between CRC tissue.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Bioprinting of Pig Macrophages and Human Cells Discovered the P2Y14 Receptor as a Mediator of Xenogenic Immune Responses. 猪巨噬细胞和人类细胞的三维生物打印发现了作为异种免疫反应介质的 P2Y14 受体。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-02 DOI: 10.1080/08820139.2024.2411388

Hyungkuen Kim, Sung-Jo Kim

{"title":"3D Bioprinting of Pig Macrophages and Human Cells Discovered the P2Y14 Receptor as a Mediator of Xenogenic Immune Responses.","authors":"Hyungkuen Kim, Sung-Jo Kim","doi":"10.1080/08820139.2024.2411388","DOIUrl":"https://doi.org/10.1080/08820139.2024.2411388","url":null,"abstract":"Background: The survival rate of pig lung xenotransplantation (PLXTx) recipients is severely limited by intense xenogenic immune responses, necessitating further insights into xenogeneic immunity and the development of models to study the PLXTx immune response.Methods: We identified regulators of PLXTx immune response Using Gene ontology analysis. We assessed the metabolic changes and protein levels in 3D4/31 pig alveolar macrophages (PAMs) through flow cytometry and immunoblotting. To induce a xenogenic immune response, we co-cultured 3D4/31-PAMs with A549 human alveolar epithelial cells and evaluated cytokine expression using qRT-PCR.Results: Gene ontology analysis identified STAT1 and alveolar macrophages as contributors to lung autoimmunity and transplant rejection. In 3D4/31-PAMs, phorbol myristate acetate-induced glycogen accumulation and cyclooxygenase-2 expression were inhibited by the P2Y14 inhibitor PPTN. Co-culturing 3D4/31-PAMs with A549 human alveolar epithelial cells via 3D bioprinting resulted in a more pronounced inflammatory response than 2D co-culture, with increased expression of genes related to the P2Y14 cascade and inflammation. This inflammatory gene expression was prevented by PPTN treatment.Conclusion: Based on these results, we propose alginate bioprinting as an in vitro model for PLXTx and suggest that P2Y14 is a key regulator of xenogeneic immune responses in PAMs.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Serum Markers that Distinguish Behcet's Disease from Idiopathic Recurrent Aphthous Stomatitis. 区分白塞氏病和特发性复发性口疮的新型血清标记物

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-02 DOI: 10.1080/08820139.2024.2410743

Mengya Zhu, Xinliang Mao, Xianqian Huang, Minzhi Gan, Keyue Zhang, Yong Chen

{"title":"Novel Serum Markers that Distinguish Behcet's Disease from Idiopathic Recurrent Aphthous Stomatitis.","authors":"Mengya Zhu, Xinliang Mao, Xianqian Huang, Minzhi Gan, Keyue Zhang, Yong Chen","doi":"10.1080/08820139.2024.2410743","DOIUrl":"https://doi.org/10.1080/08820139.2024.2410743","url":null,"abstract":"Background: Behcet's disease (BD) is a rare and recurrent autoinflammatory disorder characterized by systemic vasculitis, frequently manifested as recurrent aphthous stomatitis (RAS). We aim to identify specific serum proteins to discriminate between BD and idiopathicRAS.Method: Peripheral blood was collected from 12 BD patients, 12 idiopathic RAS patients, and 21 healthy volunteers. The serum samples underwent Tandem Mass Tag-based mass spectrometry analysis. Differentially expressed proteins (DEPs) were identified for KEGG pathway enrichment, Gene Ontology (GO), and protein-protein interaction (PPI) analyses. ELISA was utilized to verify two BD-specific DEPs in another cohort consisting of 18 BD patients, 18 idiopathic RAS patients, and 18 controls.Results: Compared with RAS serum, BD serum showed 242 DEPs. 49 proteins were differentially expressed in BD but not RAS serum compared to healthy controls. KEGG pathway and GO analyses revealed that DEPs in BD and RAS have similar biological functions and cellular distributions, featuring a significant association with pathways regulating blood coagulation and immune response. When comparing DEPs between BD and RAS, several keratins emerged as markers that distinguish RAS from BD. We also identified multiple DEPs in BD but not RAS patients. PPI analysis uncovered that lipoprotein metabolism regulators serve as hub proteins, indicating their potentially essential roles in BD pathology. In addition, ELISA results confirmed the elevated LRG1 and SOD3 levels in BD, but not RAS patients, compared to healthy donors.Conclusion: Our data uncovered novel serum proteins that distinguish BD from RAS, which may potentially be useful in BD diagnosis and treatment.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis. 类风湿关节炎患者对甲氨蝶呤治疗差异反应的 T 细胞受体汇聚特征

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI: 10.1080/08820139.2024.2381078

Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng

{"title":"Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis.","authors":"Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng","doi":"10.1080/08820139.2024.2381078","DOIUrl":"10.1080/08820139.2024.2381078","url":null,"abstract":"Background: Methotrexate (MTX) serves as the initial treatment for rheumatoid arthritis (RA). However, a substantial proportion of RA patients, estimated between 30% and 50%, do not respond positively to MTX. While the T-cell receptor (TCR) is crucial for the immune response during RA, its role in differentiating MTX responsiveness has not been thoroughly investigated.Methods: This study used next-generation sequencing to analyze the TCR β-chain complementary determining region sequences in peripheral blood mononuclear cells obtained from RA patients before MTX treatment. This study aimed to compare the characteristics of the TCR repertoire between the MTX responder and non-responder groups.Results: The study identified a significant difference in the TRBV6-6 gene (p = .003) concerning MTX treatment response. Additionally, a significant difference was found in the number of \"3\" nucleotide deletions at the 5'Jdels site (p = .023) in the VDJ rearrangement.Conclusion: These findings suggest distinct TCR repertoire characteristics between MTX responder and non-responder groups among RA patients. This discovery offers new insights into understanding the variable responses of RA patients to MTX therapy.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM). 原代小鼠骨髓衍生巨噬细胞（BMDM）的收集、分化、冷冻保存和复苏综合方案。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1080/08820139.2024.2382805

Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple

{"title":"A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM).","authors":"Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple","doi":"10.1080/08820139.2024.2382805","DOIUrl":"10.1080/08820139.2024.2382805","url":null,"abstract":"Background: The field of immunology has undoubtedly benefited from the in vitro use of cell lines for immunological studies; however, due to the \"immortal\" nature of many cell lines, they are not always the best model. Thus, direct collection and culture of primary cells from model organisms is a solution that many researchers utilize. To the best of our knowledge, there is not a singular protocol which encompasses the entire process of bone marrow cell collection through cryopreservation and resuscitation of cells from a murine model.Methods: Bone marrow cells were collected from mice with a C57BL6 genetic background. Cells were differentiated using L929 conditioned media. Cells were assessed using a combination of microscopy, differential staining, immunocytochemistry, and trypan blue. Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear S. aureus.Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear S. aureus.Conclusion: Crypopreserved and resuscitated primary murine BMDMs were viable and retained their pverall S. aureus clearance ability.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predominance of CD137⁺ And TNF-α Expressing CD8⁺ Central Memory T Cells in Mild COVID-19 Recovered Patients Upon SARS-CoV-2 Re-Exposure. 再次接触SARS-CoV-2病毒后，轻度COVID-19康复者体内CD137+和TNF-α表达的CD8+中央记忆T细胞占主导地位

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-07-12 DOI: 10.1080/08820139.2024.2376003

Rephany Fonseca Peixoto, Pedro Henrique de Sousa Palmeira, Bárbara Guimarães Csordas, Luiz Henrique Agra Cavalcante-Silva, Arthur Gomes de Andrade, Isac Almeida de Medeiros, Fátima de Lourdes Assunção Araújo de Azevedo, Robson Cavalcante Veras, Daniele Janebro, Ian P G Do Amaral, Tatjana Souza Lima Keesen

{"title":"Predominance of CD137+ And TNF-α Expressing CD8+ Central Memory T Cells in Mild COVID-19 Recovered Patients Upon SARS-CoV-2 Re-Exposure.","authors":"Rephany Fonseca Peixoto, Pedro Henrique de Sousa Palmeira, Bárbara Guimarães Csordas, Luiz Henrique Agra Cavalcante-Silva, Arthur Gomes de Andrade, Isac Almeida de Medeiros, Fátima de Lourdes Assunção Araújo de Azevedo, Robson Cavalcante Veras, Daniele Janebro, Ian P G Do Amaral, Tatjana Souza Lima Keesen","doi":"10.1080/08820139.2024.2376003","DOIUrl":"10.1080/08820139.2024.2376003","url":null,"abstract":"Introduction: Memory CD8+ T cells are essential for long-term immune protection in viral infections, including COVID-19.Methods: This study examined the responses of CD8+ TEM, TEMRA, and TCM subsets from unvaccinated individuals who had recovered from mild and severe COVID-19 by flow cytometry.Results and discussion: The peptides triggered a higher frequency of CD8+ TCM cells in the recovered mild group. CD8+ TCM and TEM cells showed heterogeneity in CD137 expression between evaluated groups. In addition, a predominance of CD137 expression in naïve CD8+ T cells, TCM, and TEM was observed in the mild recovered group when stimulated with peptides. Furthermore, CD8+ TCM and TEM cell subsets from mild recovered volunteers had higher TNF-α expression. In contrast, the expression partner of IFN-γ, IL-10, and IL-17 indicated an antiviral signature by CD8+ TEMRA cells. These findings underscore the distinct functional capabilities of each memory T cell subset in individuals who have recovered from COVID-19 upon re-exposure to SARS-CoV-2 antigens.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0