DNA methylation and transcriptome signatures of the FOXO1 gene in ankylosing spondylitis.

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2025-06-17 DOI:10.1080/08820139.2025.2517814

Xiaohan Jing, Li Fan, Yuan Wang, Yuchen Zhang, Tongbin Xue, Di Tian, Feilong Zhang, Yuting Chen, Baoming Wu, Ye Wu

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引用次数: 0

Abstract

Background: Ankylosing spondylitis (AS) is an inflammatory autoimmune disease with complex etiology. The forkhead box O (FOXO) 1 is an important transcription factor related to proliferation, homeostasis and metabolism. Notably, the involvement of methylation and the expression of mRNA in the promoter region of the FOXO1 gene in relation to AS is still not understood.

Methods: A two-stage case-control study enrolled 60 AS patients and 60 healthy controls (HCs) for integrated demographic and clinical evaluation and DNA methylation profiling. Subsequently, FOXO1 mRNA expression was comparatively assessed in 30 AS patients and 30 hCs.

Results: The methylation levels of 2 islands and 10 sites in the promoter region of FOXO1 gene were significantly different between AS patients and healthy controls. The negative correlation between the mRNA expression and the methylation level of FOXO1 gene was revealed (r_s = -0.624, p < .001). Subgroup analyses showed that male and HLA-B27(+) having high methylation level in AS patients (p = .008; p = .036). Moreover, the level of hypermethylation was positively correlated with the clinical features like ASDAS, and negatively correlated with LYM, MON, RDW and disease duration.

Conclusion: DNA methylation and transcription of FOXO1 might be related to AS susceptibility and play an important role in the etiology of AS.

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强直性脊柱炎FOXO1基因的DNA甲基化和转录组特征。

背景：强直性脊柱炎（AS）是一种病因复杂的炎症性自身免疫性疾病。叉头盒O (FOXO) 1是一个重要的与增殖、体内平衡和代谢相关的转录因子。值得注意的是，与AS相关的FOXO1基因启动子区域甲基化和mRNA表达的参与仍然不清楚。方法：一项两阶段的病例对照研究，纳入60名AS患者和60名健康对照（hc），进行综合人口统计学和临床评估以及DNA甲基化分析。随后，比较评估了30例AS患者和30例hcc患者的FOXO1 mRNA表达。结果：AS患者与健康对照组FOXO1基因启动子区2个岛和10个位点的甲基化水平存在显著差异。FOXO1基因甲基化水平与mRNA表达呈负相关(rs = -0.624, p p = 0.008；p = .036)。高甲基化水平与ASDAS等临床特征呈正相关，与LYM、MON、RDW、病程呈负相关。结论：FOXO1基因的DNA甲基化和转录可能与AS易感性有关，并在AS的病因学中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.