Immunological Investigations最新文献

Psoriasis Relapse: Exploring the Role of Epigenetics, Metabolic Reprogramming, and Inflammatory Memory. 银屑病复发：探索表观遗传学、代谢重编程和炎症记忆的作用。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-06-19 DOI: 10.1080/08820139.2025.2519666

Sakshi Wadhavane, Arulkumaran Rithvik, Mahaboobkhan Rasool

{"title":"Psoriasis Relapse: Exploring the Role of Epigenetics, Metabolic Reprogramming, and Inflammatory Memory.","authors":"Sakshi Wadhavane, Arulkumaran Rithvik, Mahaboobkhan Rasool","doi":"10.1080/08820139.2025.2519666","DOIUrl":"https://doi.org/10.1080/08820139.2025.2519666","url":null,"abstract":"Background: Psoriasis is a chronic autoimmune condition characterized by recurrent episodes of skin inflammation. Despite progress in treatment, managing flare-ups of psoriasis remains a significant hurdle once the therapy is halted. This review aims to unravel the enigma of relapse by examining the interactions between epigenetics, metabolic reprogramming, and inflammatory memory.Methods and Results: Skin-resident memory T cells and keratinocytes with a history of inflammation play crucial roles in the metabolic and epigenetic alterations observed during relapse. This review explores epigenetic factors involved in the recurrence of psoriasis, such as histone alterations, chromatin restructuring, and non-coding RNAs. Furthermore, we explored environmental influences, metabolic reprogramming, and genetic predispositions that influence the persistence and recurrence of psoriasis. We also outline the function of the gut-brain-skin axis in this scenario. Finally, we discuss pharmacological strategies for managing psoriasis relapse, including targeted biologics.Conclusion: This review provides a comprehensive summary on the intricate epigenetic, molecular, metabolic and environmental cues that exacerbate or facilitate psoriasis relapse. In summary, it also provides an enticing update on the therapeutics currently employed to treat psoriasis relapse.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-27"},"PeriodicalIF":2.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T Cells Expressing Ras Homolog Family Member a Display Enhanced Cytotoxicity but are Reduced in Lupus Peripheral Blood. 表达Ras同源家族成员a的T细胞在狼疮外周血中显示增强的细胞毒性但降低。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-06-19 DOI: 10.1080/08820139.2025.2517364

Ziqi Xiong, Zhao Guan, Ainizati Hasimu, He Yu, Sen Zhou, Ayibaota Bahabayi, Qi Li, Guochong Wang, Zhonghui Zhang, Rui Kang, Pingzhang Wang, Chen Liu

{"title":"T Cells Expressing Ras Homolog Family Member a Display Enhanced Cytotoxicity but are Reduced in Lupus Peripheral Blood.","authors":"Ziqi Xiong, Zhao Guan, Ainizati Hasimu, He Yu, Sen Zhou, Ayibaota Bahabayi, Qi Li, Guochong Wang, Zhonghui Zhang, Rui Kang, Pingzhang Wang, Chen Liu","doi":"10.1080/08820139.2025.2517364","DOIUrl":"https://doi.org/10.1080/08820139.2025.2517364","url":null,"abstract":"Objective: This study aimed to explore the expression and clinical relevance of Ras homolog family member A (RhoA) in T cell subsets from patients with systemic lupus erythematosus (SLE).Methods: Peripheral blood samples were obtained from newly diagnosed SLE patients and age- and sex-matched healthy controls. T cell subpopulations were analyzed by flow cytometry to quantify RhoA levels and associated cytotoxic markers, including granzyme B (GrB) and perforin (PFFN). Publicly available single-cell RNA sequencing (scRNA-seq) data were used to validate RhoA transcriptional patterns. Diagnostic performance was evaluated using receiver operating characteristic (ROC) curve analysis.Results: RhoA was unevenly distributed among circulating T cell populations, with the highest protein expression observed in CD8+ and effector memory subsets. RhoA+ T cells showed significantly higher GrB and PFN levels compared to their RhoA+ counterparts. In early-stage SLE, RhoA expression in T cells was significantly reduced compared to healthy individuals. However, a greater proportion of CD8+RhoA+ cells expressed GZMB in SLE patients. ROC analysis yielded area under the curve (AUC) values of 0.6720 for CD4+RhoA+ and 0.6635 for CD8+RhoA+ T cells.Conclusion: RhoA+ T lymphocytes exhibit enhanced cytotoxic potential and may serve as early immunological markers for the identification of SLE.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-14"},"PeriodicalIF":2.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation and transcriptome signatures of the FOXO1 gene in ankylosing spondylitis. 强直性脊柱炎FOXO1基因的DNA甲基化和转录组特征。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-06-17 DOI: 10.1080/08820139.2025.2517814

Xiaohan Jing, Li Fan, Yuan Wang, Yuchen Zhang, Tongbin Xue, Di Tian, Feilong Zhang, Yuting Chen, Baoming Wu, Ye Wu

{"title":"DNA methylation and transcriptome signatures of the FOXO1 gene in ankylosing spondylitis.","authors":"Xiaohan Jing, Li Fan, Yuan Wang, Yuchen Zhang, Tongbin Xue, Di Tian, Feilong Zhang, Yuting Chen, Baoming Wu, Ye Wu","doi":"10.1080/08820139.2025.2517814","DOIUrl":"https://doi.org/10.1080/08820139.2025.2517814","url":null,"abstract":"Background: Ankylosing spondylitis (AS) is an inflammatory autoimmune disease with complex etiology. The forkhead box O (FOXO) 1 is an important transcription factor related to proliferation, homeostasis and metabolism. Notably, the involvement of methylation and the expression of mRNA in the promoter region of the FOXO1 gene in relation to AS is still not understood.Methods: A two-stage case-control study enrolled 60 AS patients and 60 healthy controls (HCs) for integrated demographic and clinical evaluation and DNA methylation profiling. Subsequently, FOXO1 mRNA expression was comparatively assessed in 30 AS patients and 30 hCs.Results: The methylation levels of 2 islands and 10 sites in the promoter region of FOXO1 gene were significantly different between AS patients and healthy controls. The negative correlation between the mRNA expression and the methylation level of FOXO1 gene was revealed (rs = -0.624, p < .001). Subgroup analyses showed that male and HLA-B27(+) having high methylation level in AS patients (p = .008; p = .036). Moreover, the level of hypermethylation was positively correlated with the clinical features like ASDAS, and negatively correlated with LYM, MON, RDW and disease duration.Conclusion: DNA methylation and transcription of FOXO1 might be related to AS susceptibility and play an important role in the etiology of AS.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-15"},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of the Transcription Factor FOXP3 in Human Peripheral Blood B-Cell Subtypes (CD19+CD39+ and CD19+CD39-) and Evaluation of Their Regulatory Function. 转录因子FOXP3在人外周血b细胞亚型（CD19+CD39+和CD19+CD39-）中的表达及其调控功能评价

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-06-11 DOI: 10.1080/08820139.2025.2515411

A Cardenas-Juarez, E E Uresti-Rivera, F Ochoa-González, F I Lira-Hernández, E E Lara-Ramírez, J M Vargas-Morales, B Rivas-Santiago, D P Portales-Peréz, M H García-Hernández

{"title":"Expression of the Transcription Factor FOXP3 in Human Peripheral Blood B-Cell Subtypes (CD19+CD39+ and CD19+CD39-) and Evaluation of Their Regulatory Function.","authors":"A Cardenas-Juarez, E E Uresti-Rivera, F Ochoa-González, F I Lira-Hernández, E E Lara-Ramírez, J M Vargas-Morales, B Rivas-Santiago, D P Portales-Peréz, M H García-Hernández","doi":"10.1080/08820139.2025.2515411","DOIUrl":"https://doi.org/10.1080/08820139.2025.2515411","url":null,"abstract":"Introduction: The aim of this study was to evaluate FOXP3 expression in CD19+CD39+ and CD19+CD39- B cells, and to investigate its potential regulatory role.Methods: Peripheral B cells were obtained from 25 volunteers. FOXP3 expression at the mRNA and protein levels was analyzed in CD19+CD39+ and CD19+CD39- B cells by FACS and RT-qPCR. Suppressive activity was assessed through co-cultures of PBMC with CD19+CD39+ and CD19+CD39- B cells stimulated with anti-CD3/CD28, evaluating T cell proliferation and the percentage of Th1 cells.Results: The percentage of CD19+CD39+ FOXP3+ B cells was higher compared to other phenotypes. There was a positive correlation between FOXP3 and CD39 in CD19+ B cells. FOXP3 mRNA was increased in CD19+CD39+ B cells compared to CD19+CD39- B cells. CD19+CD39- B cells reduced the proliferation, the percentage of Th1 cells, and expressed higher IL-10 mRNA compared to CD19+CD39+ B cells. B cell phenotypes were inversely associated with Th1 cells and CRP. CD19+CD39- was associated with HOMA-β. CD19+CD39+ was inversely associated with HbA1c.Discussion: FOXP3 is expressed on both CD19+CD39- and CD19+CD39+ B lymphocytes. CD19+CD39- cells showed high levels of IL-10 and low levels of FOXP3 mRNA. CD19+CD39- B cells decreased the Th1 cells and were associated with β-cell function.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-16"},"PeriodicalIF":2.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxic ADSC Exosomes Alleviate Primary Sjögren's Syndrome-Induced Skin Injury via GLRX2 Delivery and Ferroptosis Suppression. 缺氧ADSC外泌体通过GLRX2传递和抑制铁下沉减轻原发性Sjögren综合征诱导的皮肤损伤。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-06-10 DOI: 10.1080/08820139.2025.2516671

Xin Wang, Fengjiao Wu, Xinmeng Yang, Huimin Yang, Guixia Xu, Ruilian Chen, Qinqin Xiang, Mingming Jin, Chao Sun

{"title":"Hypoxic ADSC Exosomes Alleviate Primary Sjögren's Syndrome-Induced Skin Injury via GLRX2 Delivery and Ferroptosis Suppression.","authors":"Xin Wang, Fengjiao Wu, Xinmeng Yang, Huimin Yang, Guixia Xu, Ruilian Chen, Qinqin Xiang, Mingming Jin, Chao Sun","doi":"10.1080/08820139.2025.2516671","DOIUrl":"https://doi.org/10.1080/08820139.2025.2516671","url":null,"abstract":"Background: The aim of the present study was to assess the therapeutic effects of ADSC-Exos for SS-induced skin injury.Methods: A mouse model of SS was constructed and Exos from ADSCs (Exos) and hypoxia-pretreated ADSCs (HExos) were isolated. The therapeutic effects of Exos were identified using an enzyme-linked immunosorbent assay, immunohistochemistry, and immunofluorescence. High-throughput sequencing (HTS) was employed to identify differentially expressed genes between ADSC-Exos and ADSC-HExos. The results showed that treatment with ADSC-Exos, especially ADSC-HExos, inhibited SS-induced expression of inflammatory factors, ferroptosis, and deposition of reactive oxygen species.Results: The results of HTS and polymerase chain reaction analysis revealed that GLRX2 plays an important role in protected effects of ADSC-HExo against SS-induced skin injury. In vitro analysis usingHaCaT cells confirmed that GLRX2 inhibited lipopolysaccharide-induced skin injury by inhibiting ferroptosis, as confirmed with the ferroptosis inhibitorFerrostatin-1. GLRX2 upregulation increased the therapeutic effects of ADSC-Exos against skin injury of SS mice.Conclusion: Moreover,ADSC-HExos effectively promoted collagen I expression, suggesting that ADSC-HExos attenuated primary SS-induced skin injury via GLRX2 delivery and ferroptosis suppression.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-12"},"PeriodicalIF":2.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

M1 Monocyte Polarization and Deficient Immunomodulation in Preeclampsia. 子痫前期M1单核细胞极化和免疫调节缺陷。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-06-04 DOI: 10.1080/08820139.2025.2511081

Mariana Romao-Veiga, Amanda Carreira Devides, Vanessa Rocha Ribeiro-Vasques, Mariana Leticia Matias, Virginia Juliani Gomes, Graziela Gorete Romagnoli, Maria Terezinha Serrao Peraçoli, Jose Carlos Peraçoli

{"title":"M1 Monocyte Polarization and Deficient Immunomodulation in Preeclampsia.","authors":"Mariana Romao-Veiga, Amanda Carreira Devides, Vanessa Rocha Ribeiro-Vasques, Mariana Leticia Matias, Virginia Juliani Gomes, Graziela Gorete Romagnoli, Maria Terezinha Serrao Peraçoli, Jose Carlos Peraçoli","doi":"10.1080/08820139.2025.2511081","DOIUrl":"https://doi.org/10.1080/08820139.2025.2511081","url":null,"abstract":"Introduction: Preeclampsia (PE) is a specific pregnancy syndrome characterized by a systemic inflammatory response that may be dependent on the presence of danger molecules called damage-associated molecular patterns (DAMPs). High mobility group Box 1 (HMGB1) is a DAMP that shows possible interaction with haptoglobin and can be removed from circulation by the CD163 receptor.Objective: This study aimed to evaluate the involvement of haptoglobin, HMGB1, and CD163 receptor in the systemic inflammatory response in pregnant women with PE.Methods: Monocytes obtained from preeclamptic and normotensive (NT) pregnant women were evaluated for surface TLR4, RAGE, CD64, and CD163 receptors, as well as intracellular haptoglobin and HMGB1 expression by flow cytometry.Results: Plasma levels of haptoglobin, HMGB1, and hemeoxygenase-1, as well as pro- and anti-inflammatory cytokines, were determined by the ELISA. Compared with NT group expression of TLR4, CD64, and RAGE receptors and intracellular HMGB1 and haptoglobin by monocytes was higher in women with PE, whereas extracellular CD163 expression was reduced in this group. Plasma concentrations of HMGB1, TNF-α, IL-1β, and IL-6 were higher in preeclamptic women, whereas the levels of haptoglobin, hemeoxygenase-1, and IL-10 were significantly lower.Conclusion: The elevated concentration of inflammatory cytokines and higher expression of TLR4, CD64, and RAGE receptors demonstrated that monocytes from PE women are polarized to the M1-like profile. Furthermore, the CD163 internalization and the absence of a high haptoglobin monocyte subset, along with decreased levels of haptoglobin, IL-10, and hemeoxygenase-1 in the PE group, indicates a deficiency in mechanisms that could regulate the intense inflammatory process associated with PE.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-18"},"PeriodicalIF":2.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indoleamine 2,3-Dioxygenase and Tryptophan Catabolism: Key Players in Immunosuppression and Intracellular Parasite Survival Mechanisms. 吲哚胺2,3-双加氧酶和色氨酸分解代谢：免疫抑制和细胞内寄生虫生存机制的关键参与者。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-05-30 DOI: 10.1080/08820139.2025.2511079

Lisset Torres-Martínez, Abraham U Morales-Primo, Jaime Zamora-Chimal

{"title":"Indoleamine 2,3-Dioxygenase and Tryptophan Catabolism: Key Players in Immunosuppression and Intracellular Parasite Survival Mechanisms.","authors":"Lisset Torres-Martínez, Abraham U Morales-Primo, Jaime Zamora-Chimal","doi":"10.1080/08820139.2025.2511079","DOIUrl":"https://doi.org/10.1080/08820139.2025.2511079","url":null,"abstract":"Background: Indoleamine 2,3-dioxygenase (IDO) is a heme enzyme that catalyzes the oxidative degradation of L-tryptophan (L-Trp) through the kynurenine pathway (KP), generating metabolites that regulate immune responses. These byproducts, mainly kynurenines, contribute to immunosuppression and influence immune cell differentiation, promoting regulatory T cells (Tregs) and inducing apoptosis in inflammatory cells.Methods: We conducted a comprehensive literature review to examine the roles of IDO and KP metabolites in intracellular parasitic infections. Our analysis focused on studies involving Leishmania, Trypanosoma cruzi, Toxoplasma gondii, and Plasmodium species.Results: IDO has a dual role in parasitic diseases: L-Trp depletion can inhibit parasite growth, but also promotes an immunosuppressive microenvironment that may facilitate pathogen persistence. This balance between host defense and immune evasion is crucial in chronic infections. We discuss how IDO activity intersects with parasite immune evasion strategies and review potential therapeutic approaches targeting the IDO-KP axis.Conclusion: IDO plays a complex and context-dependent role in the immunopathology of intracellular parasitic infections. While it may support host defense, its immunoregulatory effects can also favor chronic infection. Therapeutically targeting the IDO pathway is a promising strategy, but requires further investigation to optimize its clinical application.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-26"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Protein UBC13 Improves Tissue Injury of MRL/Lpr Mice by Alleviating Th1/Th2 Immune Imbalance. 重组蛋白UBC13通过缓解Th1/Th2免疫失衡改善MRL/Lpr小鼠组织损伤

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-05-30 DOI: 10.1080/08820139.2025.2509086

Mengchen Qi, Qian Li, Bingyao Ren, Ling Guo, Likang Zhang, Yulong Yang, Jiali Tao, Bin Sun, Junbing Jiang

{"title":"Recombinant Protein UBC13 Improves Tissue Injury of MRL/Lpr Mice by Alleviating Th1/Th2 Immune Imbalance.","authors":"Mengchen Qi, Qian Li, Bingyao Ren, Ling Guo, Likang Zhang, Yulong Yang, Jiali Tao, Bin Sun, Junbing Jiang","doi":"10.1080/08820139.2025.2509086","DOIUrl":"https://doi.org/10.1080/08820139.2025.2509086","url":null,"abstract":"Objectives: This study aimed to investigate the role of T-cell imbalance in the pathogenesis of systemic lupus erythematosus (SLE), the role of Ubiquitin-binding enzyme 2N (UBC13) in the treatment of SLE.Methods: Mice in the model group were intraperitoneally injected with normal saline. The UBC13 group was administered UBC13 recombinant protein, and the dexamethasone (DXM) group was treated with DXM for 8 weeks. Serum anti-dsDNA and ANA levels were quantified via ELISA. Histopathological changes were analyzed using H&E staining. Splenic mRNA expression of inflammatory cytokines and transcription factors was analyzed using qRT-PCR. Flow cytometry was used characterize Th1, Th2 and Treg cells populations, while western blotting was used to detect STAT3 and NF-κB signalling pathway-related proteins in thymic lysates.Results: UBC13 administration ameliorated splenic hyperplasia and attenuated tissue damage in MRL/lpr mice, accompanied by a reduction in serum anti-dsDNA and ANA titers. Proinflammatory cytokine production, was suppressed following UBC13 intervention. Concurrently, UBC13 restored Th1/Th2 cell equilibrium and enhanced Treg cell suppressive function. Mechanistically, UBC13 inhibited NF-κB pathway activation and suppressed STAT3 phosphorylation.Conclusion: UBC13 restores the homeostasis and function of Th1/Th2 and Treg cell through suppression of STAT3 and the NF-κB signaling pathway activation, thereby mitigating SLE-induced organ damage.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-17"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in Aqueous Humor Cytokine Concentrations According to the Presence of Posterior Subcapsular Cataract: A Prospective Case-Control Study. 后囊膜下白内障存在时房水细胞因子浓度的差异：一项前瞻性病例对照研究。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-05-29 DOI: 10.1080/08820139.2025.2512143

Kyu Seong Jo, Ji Eun Song, Yongwoo Lee

{"title":"Differences in Aqueous Humor Cytokine Concentrations According to the Presence of Posterior Subcapsular Cataract: A Prospective Case-Control Study.","authors":"Kyu Seong Jo, Ji Eun Song, Yongwoo Lee","doi":"10.1080/08820139.2025.2512143","DOIUrl":"https://doi.org/10.1080/08820139.2025.2512143","url":null,"abstract":"Background: Posterior subcapsular cataract (PSC) substantially impacts visual function, and prior studies have suggested that inflammatory cytokines may have a role in its development. We aimed to analyze inflammatory cytokine concentrations in the aqueous humor by cataract type and severity, specifically comparing them in relation to the presence of PSC, and to identify risk factors for PSC.Methods: We prospectively recruited patients undergoing routine cataract surgery. PSC presence was documented via slit-lamp examination and anterior segment photography during cataract diagnosis. Aqueous humor samples were collected prior to surgery and analyzed using quantitative multiplexed antibody assays to measure the concentrations of 10 inflammatory cytokines (interferon-α [IFN-α], IFN-γ, interleukin [IL]-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, and tumor necrosis factor-α). Cytokine concentrations were compared according to PSC status.Results: Overall,80 eyes of 80 patients were included, 45 with and 35 without PSC. Compared with those in the non-PSC group, patients with PSC showed significantly higher aqueous humor concentrations of IFN-γ (2.764 vs. 2.301 pg/mL, p = .013). No significant correlation was observed between PSC status and the aqueous humor concentrations of other examined inflammatory cytokines.Conclusion: IFN-γ expression tended to be higher in eyes with PSC than in those without PSC, suggesting a possible role in PSC pathophysiology.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-11"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aflatoxin B₁ Instigated Redox Imbalance is Accompanied by Amplified Indoleamine 2,3-Dioxygenase/tryptophan Catabolism in the Spleen and Erythrocyte of Male Wistar Rats: Protective Influence of Dietary Rutin. 黄曲霉毒素B1引起的氧化还原失衡伴随着雄性Wistar大鼠脾脏和红细胞吲哚胺2,3-双加氧酶/色氨酸分解代谢的增强：饮食芦丁的保护作用

IF 2.9 4区医学

Immunological Investigations Pub Date : 2025-05-27 DOI: 10.1080/08820139.2025.2503171

Azubuike Peter Ebokaiwe, Nworie Okoro, Doris Olachi Alilonu, Euslar Nnenna Onu, Jacinta Nkechi Obimma, ChinazomMartina Eze, Olusanya Olasehinde

{"title":"Aflatoxin B1 Instigated Redox Imbalance is Accompanied by Amplified Indoleamine 2,3-Dioxygenase/tryptophan Catabolism in the Spleen and Erythrocyte of Male Wistar Rats: Protective Influence of Dietary Rutin.","authors":"Azubuike Peter Ebokaiwe, Nworie Okoro, Doris Olachi Alilonu, Euslar Nnenna Onu, Jacinta Nkechi Obimma, ChinazomMartina Eze, Olusanya Olasehinde","doi":"10.1080/08820139.2025.2503171","DOIUrl":"https://doi.org/10.1080/08820139.2025.2503171","url":null,"abstract":"Introduction: Rutin, a dietary flavonoid, exhibits anti-inflammatory, antioxidant, and immunomodulatory properties. The underlying mechanism of protection of rutin against Aflatoxin B1 (AFB1)-induced immunotoxicity is not completely elucidated. This study investigated the protective effect of rutin against Aflatoxin B1 (AFB1)-induced immunotoxicity in male Wistar rats, supported by molecular docking and dynamics simulations.Methods: Forty male Wistar rats were grouped into five: control (corn oil), AFB1 (0.75 mg/kg bwt), AFB1 (1.5 mg/kg bwt), rutin (50 mg/kg bwt), and AFB1 (1.5 mg/kg bwt) + Rutin (50 mg/kg bwt) orally for 30 days.Results: AFB1 exposure increased (p < 0.05) oxidative and inflammatory markers, altered hematological indices, and caused histological damage in the spleen and bone marrow. Elevated indoleamine 2,3-dioxygenase (IDO) activity, reduced CD4+ T cells, and unchanged tryptophan 2,3-dioxygenase (TDO) activity were also observed. Docking revealed strong binding affinities for AFB1 (-9.5 kcal/mol), rutin (-9.7 kcal/mol), and AFB1-rutin (-10.4 kcal/mol) with IDO. Rutin co-treatment restored oxidative, inflammatory, and hematological indices, mitigated histological damage, and normalized CD4+ T cells and IDO activity, as supported by computational studies.Discussion: The activities/expression of immunosuppressive indoleamine 2, 3-dioxygenase is mostly regulated by inflammation and oxidative stress. This study provides new insights into the mechanisms underlying the modulation of immunotoxicity of AFB1 by dietary rutin.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1-23"},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0